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v-Fps-responsiveness in the Egr-1 promoter is mediated by serum response elements. 总被引:5,自引:0,他引:5 下载免费PDF全文
K Alexandropoulos S A Qureshi M Rim V P Sukhatme D A Foster 《Nucleic acids research》1992,20(9):2355-2359
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v-Src activates mitogen-responsive transcription factor Egr-1 via serum response elements 总被引:13,自引:0,他引:13
S A Qureshi X M Cao V P Sukhatme D A Foster 《The Journal of biological chemistry》1991,266(17):10802-10806
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Early growth response-1 (Egr-1) is an immediate-early gene induced by E2 in the rodent uterus and breast cancer cells. E2 induces Egr-1 mRNA and protein levels in MCF-7 human breast cancer cells and reporter gene activity in cells transfected with pEgr-1A, a construct containing the -600 to +12 region of the Egr-1 promoter linked to the firefly luciferase gene. Deletion analysis of the Egr-1 promoter identified a minimal E2-responsive region of the promoter that contained serum response element (SRE)3 (-376 to -350) which bound Elk-1 and serum response factor (SRF) in gel mobility shift assays. Hormone-responsiveness of Egr-1 in MCF-7 cells was specifically inhibited by PD98059, a mitogen-activated protein kinase kinase inhibitor, but not by LY294002, an inhibitor of phosphatidylinositol-3-kinase (PI3-K). These results contrasted with hormone-dependent activation of the SRE in the c-fos promoter, which was inhibited by both PD98059 and LY294002. Differences in activation of the SREs in Egr-1 and c-fos were related to promoter sequence, which defines the affinities of Elk-1 and SRF to their respective binding sites. Thus, Egr-1, like c-fos, is activated through non-genomic (extranuclear) pathways of estrogen action in breast cancer cells. 相似文献
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Hypoxia-associated induction of early growth response-1 gene expression. 总被引:24,自引:0,他引:24
S F Yan J Lu Y S Zou J Soh-Won D M Cohen P M Buttrick D R Cooper S F Steinberg N Mackman D J Pinsky D M Stern 《The Journal of biological chemistry》1999,274(21):15030-15040
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