共查询到20条相似文献,搜索用时 15 毫秒
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Functional interrelation of MYC2 and HY5 plays an important role in Arabidopsis seedling development
Moumita Chakraborty Sreeramaiah N. Gangappa Jay P. Maurya Vishmita Sethi Archana K. Srivastava Aparna Singh Siddhartha Dutta Madhusmita Ojha Nisha Gupta Mandar Sengupta Hasthi Ram Sudip Chattopadhyay 《The Plant journal : for cell and molecular biology》2019,99(6):1080-1097
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Fiorella Scagnoli Serena Pellegatta Federica Pisati Federico Zambelli Barbara Illi Daniela Annibali Sara Beji Elisa Orecchini Maria Adele Alberelli Clara Apicella Rosaria Anna Fontanella Alessandro Michienzi Gaetano Finocchiaro Maria Giulia Farace Giulio Pavesi Silvia Anna Ciafrè Sergio Nasi 《EMBO reports》2016,17(12):1872-1889
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Koen Bartholomeeusen Yanhui Xiang Koh Fujinaga B. Matija Peterlin 《The Journal of biological chemistry》2012,287(43):36609-36616
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《生物化学与生物物理学报:疾病的分子基础》2020,1866(12):165923
ObjectiveColorectal cancer (CRC) is one of the leading causes of cancer-related mortality. The bromodomain and extra-terminal domain (BET) inhibitors suppresses the gene expressions of various oncogenes and shows a good efficacy in the preclinical CRC models. We investigate the mechanism of action of BET inhibitors in CRC.MethodsThe effect of BET inhibitor (JQ1) on the HGF-MET signaling was assessed by qPCR, western blot and immunohistochemical staining in CRC and cancer-associated fibroblasts (CAFs). The effect of JQ1 on the CAFs was investigated using the primary CAFs derived from CRC tissues and induced-CAFs derived from isolating foreskin fibroblasts. The effect of JQ1 on the gene expression profile of CAFs was explored by RNA-sequence, qPCR and bioinformatic analysis.ResultsJQ1 decreased the mRNA and protein levels of MET in CRC cells and downregulated the mRNA and protein levels of HGF in both CRC cells and CAFs. JQ1 attenuated the pro-migratory activity of CAFs through downregulation of HGF expression in CAFs. Meanwhile, JQ1 also reduced the ability of contracting collagen gels, decreased the cell proliferation, induced G1 arrest and repressed the pro-inflammatory gene expressions in CAFs. MYC expression was suppressed by JQ1 in CAFs. Knockdown of MYC induced G1 arrest in CAFs.ConclusionOur results demonstrate the inhibitory effect of BET inhibition on the HGF-MET signaling and the pro-tumor activity of CAFs, revealing a new mechanism by which BET inhibition suppresses CRC progression. 相似文献
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Background
The Warburg effect is one of the hallmarks of cancer and rapidly proliferating cells. It is known that the hypoxia-inducible factor 1-alpha (HIF1A) and MYC proteins cooperatively regulate expression of the HK2 and PDK1 genes, respectively, in the Burkitt lymphoma (BL) cell line P493-6, carrying an inducible MYC gene repression system. However, the mechanism of aerobic glycolysis in BL cells has not yet been fully understood.Methods and Findings
Western blot analysis showed that the HIF1A protein was highly expressed in Epstein–Barr virus (EBV)-positive BL cell lines. Using biochemical assays and quantitative PCR (Q-PCR), we found that—unlike in lymphoblastoid cell lines (LCLs)—the MYC protein was the master regulator of the Warburg effect in these BL cell lines. Inhibition of the transactivation ability of MYC had no influence on aerobic glycolysis in LCLs, but it led to decreased expression of MYC-dependent genes and lactate dehydrogenase A (LDHA) activity in BL cells.Conclusions
Our data suggest that aerobic glycolysis, or the Warburg effect, in BL cells is regulated by MYC expressed at high levels, whereas in LCLs, HIF1A is responsible for this phenomenon. 相似文献9.
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While the promise of bromodomains and extraterminal (BET) protein inhibitors (BETis) is emerging in breast cancer (BC) therapy, resistance in these cells to BETis conspicuously curbs their therapeutic potential. FBW7 is an important tumour suppressor. However, the role of FBW7 in BC is not clear. In the current study, our data indicated that the low expression of FBW7 contributes to the drug resistance of BC cells upon JQ1 treatment. shRNA‐mediated FBW7 silencing in FBW7 WT BC cells suppressed JQ1‐induced apoptosis. Mechanistically, it was revealed that this diminished FBW7 level leads to Mcl‐1 stabilization, while Mcl‐1 upregulation abrogates the killing effect of JQ1. Mcl‐1 knockdown or inhibition resensitized the BC cells to JQ1‐induced apoptosis. Moreover, FBW7 knockdown in MCF7 xenografted tumours demonstrated resistance to JQ1 treatment. The combination of JQ1 with a Mcl‐1 inhibitor () resensitized the FBW7 knockdown tumours to JQ1 treatment in vivo. Our study paves the way for a novel therapeutic potential of BETis with Mcl‐1 inhibitors for BC patients with a low FBW7 expression. S63845相似文献
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Gilles Darcis Anna Kula Sophie Bouchat Koh Fujinaga Francis Corazza Amina Ait-Ammar Nadège Delacourt Adeline Melard Kabamba Kabeya Caroline Vanhulle Benoit Van Driessche Jean-Stéphane Gatot Thomas Cherrier Luiz F. Pianowski Lucio Gama Christian Schwartz Jorge Vila Arsène Burny Nathan Clumeck Michel Moutschen Stéphane De Wit B. Matija Peterlin Christine Rouzioux Olivier Rohr Carine Van Lint 《PLoS pathogens》2015,11(7)
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