Effects of prior exercise on muscle metabolism during sprint exercise in horses.

The effect of warm-up exercise on energy metabolism and muscle glycogenolysis during sprint exercise (Spr) was examined in six fit Standardbred horses exercised at 115% of maximal O(2) consumption (VO(2 max)) until fatigued, 5 min after each of three protocols: 1) no warm-up (NWU); 2) 10 min at 50% of VO(2 max) [low-intensity warm-up (LWU)]; and 3) 7 min at 50% VO(2 max) followed by 45-s intervals at 80, 90, and 100% VO(2 max) [high-intensity warm-up (HWU)]. Warm-up increased (P < 0.0001) muscle temperature (T(m)) at the onset of Spr in LWU (38.3 +/- 0.2 degrees C) and HWU (40.0 +/- 0. 3 degrees C) compared with NWU (36.6 +/- 0.2 degrees C), and the rate of rise in T(m) during Spr was greater in NWU than in LWU and HWU (P < 0.01). Peak VO(2) was higher and O(2) deficit lower (P < 0. 05) when Spr was preceded by warm-up. Rates of muscle glycogenolysis were lower (P < 0.05) in LWU, and rates of blood and muscle lactate accumulation and anaerobic ATP provision during Spr were lower in LWU and HWU compared with NWU. Mean runtime (s) in LWU (173 +/- 10 s) was greater than HWU (142 +/- 11 s) and NWU (124 +/- 4 s) (P < 0. 01). Warm-up was associated with augmentation of aerobic energy contribution to total energy expenditure, decreased glycogenolysis, and longer run time to fatigue during subsequent sprint exercise, with no additional benefit from HWU vs. LWU.     

Activity of mono- and biarticular leg muscles during sprint running

A cinematographic recording of the movements of the lower limbs together with simultaneous emg tracings from nine lower limb muscles were obtained from two male track sprinters during three phases of a 100 m sprint run. The extensor muscles of the hip joint were found to be the primary movers by acceleration of the body's center of gravity (C.G.) during the ground phase of the running cycle. The extensors of the knee joint were also important in this, but to a minor extent, while the plantar flexors of the ankle joint showed the least contribution. The biarticular muscles functioned in a way different from the monoarticular muscles in the sense that they perform eccentric work during the flight and recovery phases and concentric work during the whole ground phase (support), whereas the monoarticular muscles are restricted first to eccentric work and then to concentric work during the ground phase. Furthermore, the biarticular muscles show variation (and rate of variation) in muscle length to a larger extent than the monoarticular muscles. Paradoxical muscle actions appear to take place around the knee joint, where the hamstring muscles, m. gastrocnemius, m. vastus laterialis and m. vastus medialis act as synergists by extending the knee joint during the last part of the ground phase.     

Skeletal muscle metabolism during high-intensity sprint exercise is unaffected by dichloroacetate or acetate infusion.

This study investigated whether increased provision of oxidative substrate would reduce the reliance on nonoxidative ATP production and/or increase power output during maximal sprint exercise. The provision of oxidative substrate was increased at the onset of exercise by the infusion of acetate (AC; increased resting acetylcarnitine) or dichloroacetate [DCA; increased acetylcarnitine and greater activation of pyruvate dehydrogeanse (PDH-a)]. Subjects performed 10 s of maximal cycling on an isokinetic ergometer on three occasions after either DCA, AC, or saline (Con) infusion. Resting PDH-a with DCA was increased significantly over AC and Con trials (3.58 +/- 0.4 vs. 0.52 +/- 0.1 and 0.74 +/- 0.1 mmol. kg wet muscle(-1). min(-1)). DCA and AC significantly increased resting acetyl-CoA (35.2 +/- 4.4 and 22.7 +/- 2.9 vs. 10.2 +/- 1.3 micromol/kg dry muscle) and acetylcarnitine (12.9 +/- 1.4 and 11.0 +/- 1.0 vs. 3.3 +/- 0.6 mmol/kg dry muscle) over Con. Resting contents of phosphocreatine, lactate, ATP, and glycolytic intermediates were not different among trials. Average power output and total work done were not different among the three 10-s sprint trials. Postexercise, PDH-a in AC and Con trials had increased significantly but was still significantly lower than in DCA trial. Acetyl-CoA did not increase in any trial, whereas acetylcarnitine increased significantly only in DCA. Exercise caused identical decreases in ATP and phosphocreatine and identical increases in lactate, pyruvate, and glycolytic intermediates in all trials. These data suggest that there is an inability to utilize extra oxidative substrate (from either stored acetylcarnitine or increased PDH-a) during exercise at this intensity, possibly because of O(2) and/or metabolic limitations.     

Effect of creatine supplementation on sprint exercise performance and muscle metabolism

The aim of thepresent study was to examine the effect of creatine supplementation(CrS) on sprint exercise performance and skeletal muscle anaerobicmetabolism during and after sprint exercise. Eight active, untrainedmen performed a 20-s maximal sprint on an air-braked cycle ergometerafter 5 days of CrS [30 g creatine (Cr) + 30 g dextrose perday] or placebo (30 g dextrose per day). The trials wereseparated by 4 wk, and a double-blind crossover design was used. Muscleand blood samples were obtained at rest, immediately after exercise,and after 2 min of passive recovery. CrS increased the muscle total Crcontent (9.5 ± 2.0%, P < 0.05, mean ± SE); however, 20-s sprint performance was not improved byCrS. Similarly, the magnitude of the degradation or accumulation ofmuscle (e.g., adenine nucleotides, phosphocreatine, inosine 5'-monophosphate, lactate, and glycogen) and plasma metabolites (e.g., lactate, hypoxanthine, and ammonia/ammonium) were also unaffected by CrS during exercise or recovery. These data demonstrated that CrS increased muscle total Cr content, but the increase did notinduce an improved sprint exercise performance or alterations inanaerobic muscle metabolism.

     

Creatine supplementation and multiple sprint running performance

The aim of this study was to examine the effects of short-term creatine monohydrate supplementation on multiple sprint running performance. Using a double-blind research design, 42 physically active men completed a series of 3 indoor multiple sprint running trials (15 x 30 m repeated at 35-second intervals). After the first 2 trials (familiarization and baseline), subjects were matched for fatigue score before being randomly assigned to 5 days of either creatine (4 x d(-1) x 5 g creatine monohydrate + 1 g maltodextrin) or placebo (4 x d(-1) x 6 g maltodextrin) supplementation. Sprint times were recorded via twin-beam photocells, and earlobe blood samples were drawn to evaluate posttest lactate concentrations. Relative to placebo, creatine supplementation resulted in a 0.7 kg increase in body mass (95% likely range: 0.02 to 1.3 kg) and a 0.4% reduction in body fat (95% likely range: -0.2 to 0.9%). There were no significant (p > 0.05) between-group differences in multiple sprint measures of fastest time, mean time, fatigue, or posttest blood lactate concentration. Despite widespread use as an ergogenic aid in sport, the results of this study suggest that creatine monohydrate supplementation conveys no benefit to multiple sprint running performance.     

Estimation of temporal parameters during sprint running using a trunk-mounted inertial measurement unit

The purpose of this study was to identify consistent features in the signals supplied by a single inertial measurement unit (IMU), or thereof derived, for the identification of foot-strike and foot-off instants of time and for the estimation of stance and stride duration during the maintenance phase of sprint running. Maximal sprint runs were performed on tartan tracks by five amateur and six elite athletes, and durations derived from the IMU data were validated using force platforms and a high-speed video camera, respectively, for the two groups. The IMU was positioned on the lower back trunk (L1 level) of each athlete. The magnitudes of the acceleration and angular velocity vectors measured by the IMU, as well as their wavelet-mediated first and second derivatives were computed, and features related to foot-strike and foot-off events sought. No consistent features were found on the acceleration signal or on its first and second derivatives. Conversely, the foot-strike and foot-off events could be identified from features exhibited by the second derivative of the angular velocity magnitude. An average absolute difference of 0.005 s was found between IMU and reference estimates, for both stance and stride duration and for both amateur and elite athletes. The 95% limits of agreement of this difference were less than 0.025 s. The results proved that a single, trunk-mounted IMU is suitable to estimate stance and stride duration during sprint running, providing the opportunity to collect information in the field, without constraining or limiting athletes' and coaches' activities.     

Effect of short-term sprint interval training on human skeletal muscle carbohydrate metabolism during exercise and time-trial performance.

Our laboratory recently showed that six sessions of sprint interval training (SIT) over 2 wk increased muscle oxidative potential and cycle endurance capacity (Burgomaster KA, Hughes SC, Heigenhauser GJF, Bradwell SN, and Gibala MJ. J Appl Physiol 98: 1895-1900, 2005). The present study tested the hypothesis that short-term SIT would reduce skeletal muscle glycogenolysis and lactate accumulation during exercise and increase the capacity for pyruvate oxidation via pyruvate dehydrogenase (PDH). Eight men [peak oxygen uptake (VO2 peak)=3.8+/-0.2 l/min] performed six sessions of SIT (4-7x30-s "all-out" cycling with 4 min of recovery) over 2 wk. Before and after SIT, biopsies (vastus lateralis) were obtained at rest and after each stage of a two-stage cycling test that consisted of 10 min at approximately 60% followed by 10 min at approximately 90% of VO2 peak. Subjects also performed a 250-kJ time trial (TT) before and after SIT to assess changes in cycling performance. SIT increased muscle glycogen content by approximately 50% (main effect, P=0.04) and the maximal activity of citrate synthase (posttraining: 7.8+/-0.4 vs. pretraining: 7.0+/-0.4 mol.kg protein -1.h-1; P=0.04), but the maximal activity of 3-hydroxyacyl-CoA dehydrogenase was unchanged (posttraining: 5.1+/-0.7 vs. pretraining: 4.9+/-0.6 mol.kg protein -1.h-1; P=0.76). The active form of PDH was higher after training (main effect, P=0.04), and net muscle glycogenolysis (posttraining: 100+/-16 vs. pretraining: 139+/-11 mmol/kg dry wt; P=0.03) and lactate accumulation (posttraining: 55+/-2 vs. pretraining: 63+/-1 mmol/kg dry wt; P=0.03) during exercise were reduced. TT performance improved by 9.6% after training (posttraining: 15.5+/-0.5 vs. pretraining: 17.2+/-1.0 min; P=0.006), and a control group (n=8, VO2 peak=3.9+/-0.2 l/min) showed no change in performance when tested 2 wk apart without SIT (posttraining: 18.8+/-1.2 vs. pretraining: 18.9+/-1.2 min; P=0.74). We conclude that short-term SIT improved cycling TT performance and resulted in a closer matching of glycogenolytic flux and pyruvate oxidation during submaximal exercise.     

Can cycle power predict sprint running performance?

A major criticism of present models of the energetics and mechanics of sprint running concerns the application of estimates of parameters which seem to be adapted from measurements of running during actual competitions. This study presents a model which does not perpetuate this solecism. Using data obtained during supra-maximal cycle ergometer tests of highly trained athletes, the kinetics of the anaerobic and aerobic pathways were modelled. Internal power wasted in the acceleration and deceleration of body limbs and the power necessary to overcome air friction was calculated from data in the literature. Assuming a mechanical efficiency as found during submaximal cycling, a power equation was constructed which also included the power necessary to accelerate the body at the start of movement. The differential equation thus obtained was solved through simulation. The model appeared to predict realistic times at 100 m (10.47 s), 200 m (19.63 s) and 400 m (42.99 s) distances. By comparison with other methods it is argued that power equations of locomotion should include the concept of mechanical efficiency.     

Familiarization and reliability of multiple sprint running performance indices

The aims of this study were to evaluate the time-course of the familiarization process associated with a test of multiple sprint running performance and to determine the reliability of various performance indices once familiarization had been established. Eleven physically active men (mean age: 21 +/- 2 years) completed 4 multiple sprint running trials (12 x 30 m; repeated at 35-s intervals) with 7 days between trials. All testing was conducted indoors, and times were recorded by twin-beam photocells. Results revealed no apparent learning effects as evidenced by no significant (p > 0.05) between-trial differences in measures of fastest or mean 30-m sprint time. Within-subject test-retest reliability determined over 4 trials by coefficient of variation (CV) and intraclass correlation coefficient (ICC) showed excellent reliability for measures of fastest and mean sprint times (CV range: 1.34-2.24%; ICC range: 0.79-0.94). Pre- and posttrial blood lactate concentrations showed good reliability when judged in context with typical values (CV range: 12.08-18.21%; ICC range: 0.72-0.78). In contrast, and in line with previous research, fatigue data showed much greater variability (CV: 26.43%; ICC: 0.66). The results of this study suggest that high degrees of test-retest reliability can be obtained in many multiple sprint running indices without the need for prior familiarization.     

The effects of sprint running training on sloping surfaces

The aim of this study was to examine the effects of sprint running training on sloping surfaces (3 degrees ) on selected kinematic and physiological variables. Thirty-five sport and physical education students were randomized into 4 training groups (uphill-downhill, downhill, uphill, and horizontal) and a control group, with 7 participants in each group. Pre- and posttraining tests were performed to examine the effects of 6 weeks of training on the maximum running speed at 35 m, step rate, step length, step time, contact time, eccentric and concentric phase of contact time, flight time, selected posture characteristics of the step cycle, and peak anaerobic power performance. Maximum running speed and step rate were increased significantly (p < 0.05) in a 35-m running test after training by 0.29 m.s(-1) (3.5%) and 0.14 Hz (3.4%) for the combined uphill-downhill group and by 0.09 m.s(-1) (1.1%) and 0.03 Hz (2.4%) for the downhill group, whereas flight time shortened only for the combined uphill-downhill training group by 6 milliseconds (4.3%). There were no significant changes in the horizontal and control groups. Overall, the posture characteristics and the peak anaerobic power performance did not change with training. It can be suggested that the novel combined uphill-downhill training method is significantly more effective in improving the maximum running velocity at 35 m and the associated horizontal kinematic characteristics of sprint running than the other training methods are.     

Effect of pre-performance lower-limb massage on thirty-meter sprint running

Massage is a commonly utilized therapy within sports, frequently intended as an ergogenic aid prior to performance. However, evidence as to the efficacy of massage in this respect is lacking, and massage may in some instances reduce force production. The aim of this study was to investigate the effect of massage on subsequent 30-m sprint running performance. Male university level repeat sprint sports players volunteered for the study (n = 37). After each of 3 treatment conditions, subjects completed a standardized warm-up followed by three 30-m sprint trials in a counterbalanced crossover design. Treatment conditions were 15 minutes of lower-limb massage (M), 15 minutes of placebo ultrasound (PU), and rest (R). Thirty-meter sprint times were recorded (including 10-m split times) for the 3 trials under each condition. Best times at 10 m (M: 1.85 +/- 0.09 seconds, PU: 1.84 +/- 0.11 seconds, R: 1.83 +/- 0.10 seconds) and 30 m (M: 4.41 +/- 0.27 seconds, PU: 4.39 +/- 0.28 seconds, R: 4.39 +/- 0.28 seconds) were not significantly different (p > 0.05). There was no significant treatment, trial, or interaction effect for 10- or 30-m sprint times (p > 0.05). No difference was seen in the location of subjects' best times across the 3 trials (p > 0.05). Relative to placebo or control, the results of this study showed that a controlled 15-minute lower-limb massage administered prior to warm-up had no significant effect on subsequent 30-m sprint performance. Massage remains indicated prior to performance where other benefits, such as reduced muscle spasm and psychological stress, might be served to the athlete.     

Segment-interaction analysis of the stance limb in sprint running

A high angular velocity of the thigh of the stance limb, generated by hip extensor musculature, is commonly thought to be a performance-determining factor in sprint running. However, the thigh segment is a component of a linked system (i.e., the lower limb), therefore, it is unlikely that the kinematics of the thigh will be due exclusively to the resultant joint moment (RJM) at the hip. The purpose of this study was to quantify, by means of segment-interaction analysis, the determinants of sagittal plane kinematics of the lower limb segments during the stance phase of sprint running. Video and ground reaction force data were collected from four male athletes performing maximal-effort sprints. The analysis revealed that during the first-third of the stance phase, a hip extension moment was the major determinant of the increasing angular velocity of the thigh. However, during the mid-third of stance, hip and knee extension moments and segment interaction effects all contributed to the thigh attaining its peak angular velocity. Extension moments at the ankle, and to a lesser extent the knee, were attributed with preventing the 'collapse' of the shank under the effects of the interactive moment due to ground reaction force. The angular acceleration of the foot was determined almost completely by the RJM at the ankle and the interactive moment due to ground reaction force. Further research is required to determine if similar results exit for a wide range of athletes and for other stages of a sprint race (e.g. early acceleration, maximal velocity, and deceleration phases).     

Lower extremity muscle activation during horizontal and uphill running

Sloniger, Mark A., Kirk J. Cureton, Barry M. Prior, andEllen M. Evans. Lower extremity muscle activationduring horizontal and uphill running. J. Appl.Physiol. 83(6): 2073-2079, 1997.To provide more comprehensive information on theextent and pattern of muscle activation during running, we determinedlower extremity muscle activation by using exercise-induced contrastshifts in magnetic resonance (MR) images during horizontal and uphillhigh-intensity (115% of peak oxygen uptake) running to exhaustion(2.0-3.9 min) in 12 young women. The mean percentage of musclevolume activated in the right lower extremity was significantly(P <0.05) greater during uphill (73 ± 7%) than during horizontal (67 ± 8%) running. Thepercentage of 13 individual muscles or groups activated varied from 41 to 90% during horizontal running and from 44 to 83% during uphillrunning. During horizontal running, the muscles or groups mostactivated were the adductors (90 ± 5%), semitendinosus (86 ± 13%), gracilis (76 ± 20%), biceps femoris (76 ± 12%), andsemimembranosus (75 ± 12%). During uphill running, the musclesmost activated were the adductors (83 ± 8%), biceps femoris (79 ± 7%), gluteal group (79 ± 11%), gastrocnemius (76 ± 15%), and vastus group (75 ± 13%). Compared with horizontalrunning, uphill running required considerably greater activation of thevastus group (23%) and soleus (14%) and less activation of the rectusfemoris (29%), gracilis (18%), and semitendinosus (17%). We concludethat during high-intensity horizontal and uphill running to exhaustion,lasting 2-3 min, muscles of the lower extremity are not maximallyactivated, suggesting there is a limit to the extent to whichadditional muscle mass recruitment can be utilized to meet the demandfor force and energy. Greater total muscle activation during exhaustive uphill than during horizontal running is achieved through an altered pattern of muscle activation that involves increased use of some muscles and less use of others.

     

Reproducibility of gastrocnemius medialis muscle architecture during treadmill running

The purpose of this study was to assess the reproducibility of fascicle length (FL) and pennation angle (PA) of gastrocnemius medialis (GM) muscle during running in vivo. Twelve male recreational long distance runners (mean ± SD; age: 24 ± 3 years, mass: 76 ± 7 kg) ran on a treadmill at a speed of 3.0 m/s, wearing their own running shoes, for two different 10 min sessions that were at least 2 days apart. For each test day 10 acceptable trials were recorded. Ankle and knee joint angle data were recorded by a Vicon 624 system with three cameras operating at 120 Hz. B-mode ultrasonography was used to examine fascicle length and pennation angle of gastrocnemius medialis muscle. The ultrasound probe was firmly secured on the muscle belly using a lightweight foam fixation. The results indicated that fascicle length and pennation angle demonstrated high reproducibility values during treadmill running both for within and between test days. The root mean square scores between the repeated waveforms of pennation angle and fascicle length were small (∼2° and ∼3.5 mm, respectively). However, ∼14 trials for pennation angle and ∼9 trials for fascicle length may be required in order to record accurate data from muscle architecture parameters. In conclusion, ultrasound measurements may be highly reproducible during dynamic movements such as treadmill running, provided that a proper fixation is used in order to assure the constant location and orientation of the ultrasound probe throughout the movement.