Temporal and spatial distribution of the ichthyotoxic dinoflagellate Gyrodinium aureolum Hulburt in the St Lawrence, Canada

The occurrence of the potentially ichthyotoxic dinoflagellateGyrodinium aureolum Hulburt is reported for the first time inthe Lower Estuary and Gulf of St Lawrence. Taxonomic identificationusing an iramunochemical tagging method suggests a close taxonomicproximity between the species found in the St Lawrence and G.aureolumpresent in European waters. In 1993, G.aureolum was observedalong the entire coast of Quebec and in offshore waters of theGulf of St Lawrence. At the coastal stations, G.aureolum showedlarge week-to-week variations, but higher densities were observedduring late August and early September. Bloom concentrations(10⁶ cells 1^–1) were only observed in the GaspéCurrent at Mont-Louis, on the north coast of the Gaspd peninsula.In the central part of the Gulf, G.aureolum was concentratedin the upper 5 m of the stations directly influenced by thefreshwater run-off of the St Lawrence Estuary. Results fromprincipal component analysis indicate that G.aureolum favorsenvironments with high nitrogen recycling activity.     

Observations on pigments and morphology of Gyrodinium aureolum Hulburt, a marine dinoflagellate containing 19' -hexanoyloxyfucoxanthin as the main carotenoid

Gyrodinium aureolum, a common "red tide" dinoflagellate in Europeanwaters often associated with fish mortality, was isolated fromthe Oslofjord, Norway, and analysed for chlorophylls and carotenoids.Besides chlorophyll a and c the following carotenoids were characterizedby thin-layer chromatography, visible light spectrophotometryand mass spectrometry: ß,-carotene, ß,ß-carotene,djatoxanthin, diadinoxanthin, 19'-hexanoyloxyfucoxanthin and3 xanthophylls which could not be correlated with hitherto structurallyknown carotenoids from dinoflagellates. G. aureolum deviatesfrom most dinoflagellates by the lack of peridinin, but showsaffinity with Gyrodinium sp.-A by the possession of 19'-hexanoyloxyfucoxanthin. Preliminary light microscopical observations on the internalstructure indicate that G. aureolum is uni-nucleate with a typicaldinokaryotic nucleus containing continually condensed chromosomes.The chloroplasts seem to possess an internal pyrenoid like someother dinoflagellates with deviating carotenoid pigmentation.The similarity in carotenoid pigmentation and chloroplast structureof Emiliania huxleyi (Prymnesiophyceae) and Gyrodinium sp.-Aand G.aureolum (Dinophyceae) is pointed out. The potential chemotaxonomicvalue of the carotenoid composition in establishing identitywith morphologically similar and ichthyotoxic dinoflagellatesis briefly discussed.     

Temperature and salinity effect on growth and chemical composition of Gyrodinium aureolum Hulburt in culture

A factorial experiment shows highly significant effects of temperature(12 5–22.5°C) and salinity (17.8–34 S) on thegrowth rate of Gyrodinium aureolum, with a significant temperature-salinityinteraction. The maximum growth rate of G aureolum is measuredto 0.61 div. day^–1 at 20°C and 22.3 S. Gyrodiniumaureolum does not grow at temperatures :10 °C or 25°Cand at salinities 12 S. The cellular content of carbon (C) andnitrogen (N) and the elemental ratios N/C, P/C and N/P are significantlyaffected by the temperature The cellular content of phosphorus(P) and the elemental ratios P/C and N/P vary significantlywith salinity Significant temperature-salinity interactionsare found for the cellular content of carbon, nitrogen and phosphorus.Variations in the N/P ratio indicate that G.aureolum has a largestorage capacity for phosphorus It is suggested that temperatureis one important limiting factor in the initiation of bloomsof G.aureolum in north European waters.     

Photomovement and Photobleaching In Two Gyrodinium Species

The phototactic orientation in Gyrodinium dorsum and G. aureolumhas been analyzed. Both species show only positive phototaxiswith an optimum at about 300 W-m^–2. The mechanism of photoorientationdoes not seem to be based on a dichroic orientation of the photoreceptorpigments as in the flagellate Euglena gracilis. Photobleachingexperiments have shown a far higher resistance toward continuousirradiation at even high fluence rates than in other flagellateswhich is in good agreement with the exclusive behavior of positivephototaxis. (Received August 7, 1987; Accepted July 14, 1988)     

Ontogeny of growth, respiration and feeding rate of the freshwater calanoid copepod Eudiaptomus graciloides

The calanoid copepod, Eudiaplomus graciloides, was reared fromegg to adult on uni-algal diets (0.1. 0.5 and 2.5 mg dry wt1^–1) using the green alga, Chlamydomonas reinhardtii,as food, or on a mixed diet consisting of Lake Esrom water filteredthrough a plankton net with pore size 45 µm and supplementedwith C. reinhardtii (2.5 mg dry wt 1^–1). On the mixeddiet at 21.0°C growth in body dry wt (W, µg dry wt)was exponential, and the growth constants were 0.21 day^–1in the early to mid juvenile stage (N1 - C4) and 0.11 day^–1in the late juvenile to early adult stage (C4-A). At 14.5°Cthe corresponding growth rate constants were 0.10 and 0.08 day^–1.Similar growth rates were found at uni-algal concentrationsof 0.5 and 2.5 mg dry wt I^–1, and it was argued that thethreshold concentration for growth in Eudiaptomus was closeto 0.1 mg dry wt I^–1. The clearance (C, ml h^–1)of copepodites was measured on the uni-algal diets. The constantsof the regression (C = aW^b) were: a = 0.125, b = 0.858 (2000C. reinhardtii ml^–1), a = 0.068, b = 0.849 (10 000), a= 0.028, b = 0.875 (50 000). Ingestion rates were calculatedfrom the clearances and the average algal concentrations. Atthe three food levels the average daily rations were 30, 67and 125% of body dry wt. The respiration rate (R, nl O₂ h^–1)was measured in individuals reared on the mixed diet. The constantsof the regression (R = aW^b) were: a = 4.82, b = 1.07 (nauplii,14.5°C), a = 4.17, b = 0.904 (copepodites and adults, 14.5°C),a = 6.87, b = 0.757 (copepodites and adults, 21.0°C). Nosignificant difference in the respiration rate of copepoditesreared on uni-algal diets and the mixed diet could be demonstrated.Energy budgets were calculated. The assimilation efficiencyand the gross growth efficiency of copepodites decreased markedlywith increasing food concentration, the net growth efficiencyvaried from an average of 0.44 at the lowest algal concentrationto 0.60 on the mixed diet. The results are discussed in relationto previous findings with both freshwater and marine copepods.     

Application of Flow Microflorometry to the Study of Algal Cells and Isolated Chloroplasts

The applicability of flow microfluorometry (FMF) to the studyof chlorophyll-containing cells was investigated through theuse of the blue-green alga Agmenellum quadruplicatum, the greenalgae Trebouxia, Chlorella, and Euglena spp., and isolated spinachchloroplasts. When excited by laser radiation (488 nm), algalcells emitted fluorescence with intensity positively relatedto the chloro-phyll content. The chlorophyll fluorescent signalswere used further as a differential criterion in determiningrelative size based on light scattering logic and to sort mixturesof algal cells having different chlorophyll content The FMFalso was useful in estimating nucleic acid and protein contentin completely dechlorophyUized algal cells with the use of ethidiumbromide (EB) and fluoresceinisothiocyanate (FITC), respectively.     

Acetate metabolism in the process of "acetate-bleaching" of Chlorella protothecoides

Green cells of Chlorella protothecoides when incubated in amedium containing acetate but no nitrogen source, have beenshown to be bleached as strongly as in glucose-induced bleaching.Using U-¹⁴C-acetate as tracer, the acetate metabolism of algalcells during the process of acetate-induced bleaching was investigated.Changes in algal cell activities for respiration and assimilationof added ¹⁴C-acetate were followed during bleaching processesin "acetate-adapted" and "non-adapted" green cells. As in glucose-inducedbleaching of algal cells, algal cell activity for incorporating¹⁴C into lipids showed the most characteristic change, suggestingthat lipogenesis is causally related to the occurrence of bleachingin algal cells. (Received March 5, 1969; )     

Fluorescence characteristics of cyanobacteria (blue-green algae)

The fluorescence characteristics of the cyanobacteria Synechocystisaquatilis Sauv., Microcystis firma (Breb. et Lenorm.) Schmidleand Synechococcus leopoliensis (Racib.) Kom. and the green algaScenedesmus quadricauda (Turp.) Breb. were examined. In thethree cyanobacteria, phycocyanin is the main accessory pigment.Phycoerythrin is not present in our investigated strains ofcyanobacteria. The highest excitation of the chlorophyll a (Chla) fluorescence of cyanobacteria resulted from light with wavelengthsof 620–630 nm. A definite ‘Kautsky’ effectis also evident at this wavelength. However, excitation withblue light (420–520 nm) produced only very slight fluorescence.The Kautsky effect is not evident at these wavelengths, evenat high photon flux densities. For Scenedesmus, fluorescencecharacteristics typical of green algae were found. The fluorescenceexcitation of cyanobacteria at 620 nm corresponds to a photosynthesispeak in the action spectrum measured in terms of O₂ production.The results underline the necessity of fluorescence measurementsat several wavelengths whenever mixed populations are involved.Such measurements also present possibilities for more accurateestimation of biomass and potential photosynthetic productionin mixed populations.     

Use of immobilized algae for estimating bioavailable phosphorus released by zooplankton

Cells of the green alga Selenastrum capricomuxum were immobilizedin permeable alginate beads to prevent them from being grazedby zooplankton. Algae were able to grow in these beads and wereused as a new technique to estimate bioavailable phosphorus(P) released by zooplankton. P-limited algal cells were encapsulatedin alginate beads and used to measure P-release by Daphnia pulexfeeding on P-saturated and P-limited free algal cells. Daphnidsgrazing on P-saturated cells released 20 times more P availablefor the immobilized algae than animals grazing on P-limitedcells (0.06 versus 0.003 µg P mg^–1 Daphnia-DW h^–1).     

An unusual bloom of Gyrodinium cf. aureolum in the Argentine sea: community structure and conditioning factors

In October 1988 a bloom of Gyrodinium cf. aureolum was recordedalong a transect across the continental shelf reaching celldensities of 1300 ml^–1 and chlorophyll a concentrationsup to 16 µg 1^–1. The bloom was restricted to a nutrient-richthin surface layer of 10 m depth at the beginning of seasonalpycnocline. Hydrological conditions showed a particular salinitydistribution. The phytoplankton community was dominated by G.cf. aureolum but the coexisting flora presented a great diversity.UV-absorbing pigments were recorded in the community.     

Composition and distribution of the pelagic and sympagic algal assemblages in the Laptev Sea during autumnal freeze-up

The phytoplankton and ice algal assemblages in the SiberianLaptev Sea during the autumnal freeze-up period of 1995 aredescribed. The spatial distribution of algal taxa (diatoms,dinoflagellates, chrysophytes, chlorophytes) in the newly formedice and waters at the surface and at 5 m depth differed considerablybetween regions. This was also true for algal biomass measuredby in situ fluorescence, chlorophyll (Chl) a and taxon-specificcarbon content. Highest in situ fluorescence and Chl a concentrations(ranging from 0.1 to 3.2 µg l^–1) occurred in surfacewaters with maxima in Buor Khaya Bay east of Lena Delta. Thealgal standing stock on the shelf consisted mainly of diatoms,dinoflagellates, chrysophytes and chlorophytes with a totalabundance (excluding unidentified flagellates <10 µm)in surface waters of 351–33 660 cells l^–1. Highestalgal abundance occurred close to the Lena Delta. Phytoplanktonbiomass (phytoplankton carbon; PPC) ranged from 0.1 to 5.3 µgC l^–1 in surface waters and from 0.3 to 2.1 µg Cl^–1 at 5 m depth, and followed the distribution patternof abundances. However, the distribution of Chl a differed considerablyfrom the distribution pattern shown by PPC. The algal assemblagein the sea ice, which could not be quantified due to high sedimentload, was dominated by diatom species, accompanied by dinoflagellates.Thus, already during the early stage of autumnal freeze-up,incorporation processes, selective enrichment and subsequentgrowth lead to differences between surface water and sea icealgal assemblages.     

BIO-OPTICAL CHARACTERISTICS AND PHOTOADAPTIVE RESPONSES IN THE TOXIC AND BLOOM-FORMING DINOFLAGELLATES GYRODINIUM AUREOLUM,GYMNODINIUM GALATHEANUM,AND TWO STRAINS OF PROROCENTRUM MINIMUM1

Photoadaptive responses in the toxic and bloom-forming dinoflagellates Gyrodinium aureolum Hulbert, Gymnodinium galatheanum Braarud, and two strains of Prorocentrum minimum (Pavillard)Schiller were evaluated with respect to pigment composition, light-harvesting characteristics, carbon and nitrogen contents, and growth rates in shade- and light-adapted cells. The two former species were grown at scalar irradiances of 30 and 170 μmol · m ^?2 at a 12-h daylength at 20° C. The two strains of P. minimum were grown at 35 and 500 μmol. m^?2· s^?1 at a 2-h daylength at 20° C. For the first time, chlorophyll (chl) c₃, characteristic of several bloom-forming prymnesiophytes, was detected in G. aureolum and G. galatheanum. Photoadaptional status affected the pigment composition strongly, and the interpretation of the variation depended on whether the pigment composition was normalized per cell, carbon, or chl a. Species-specific and photoadaptional differences in chl a-specific absorption (°a_c, 400–700 nm) and chl a-normalized fluorescence excitation spectra of photosystem II fluorescence with or without addition of DCMU (°F and °F_DCMU 400–700 nm) were evident. Gyrodinium aureolum and G. galatheanum exhibited in vivo spectral characteristics similar to chl c₃-containing prymnesiophytes in accordance with their similar pigmentation. Prorocentrum minimum had in vivo absorption and fluorescence characteristics typical for peridinin-containing dinoflagellates. Species-specific differences in in vivo absorption were also observed as a function of package effect vs. growth irradiance. This effect could be explained by differences in intracellular pigment content, cell size/shape, and chloroplast morphology/numbers. Light- and shade-adapted cells of P. minimum contained 43 and 17% of photoprotective carotenoids (diadino + diatoxanthin) relative to chl a, respectively. The photoprotective function of these carotenoids was clearly observed as a reduction in °F and °F ^DCMU at 400–540 nm compared to °ac in light-adapted cells of P. minimum. Spectrally weighted light absorption (normalized to chl a and carbon, 400–700 nm) varied with species and growth conditions. The use of quantum-corrected and normalized fluorescence excitation spectra with or without DCMU-treated cells to estimate photosynthetically usable light is discussed. The usefulness of in vitro absorption and fluorescence excitation spectra for estimation of the degradation status of chl a and the ratio of chl a to total pigments is also discussed.     

Steady-state rotifer growth in a two-stage, computer-controlled turbidostat

Steady-state populations of rotifers (Brachionus calyciflorus)were maintained in twostage, continuous-flow turbidostatic cultureon the green alga Chlorella pyrenoidosa. In this system, themaximum specific growth rate,µ^max of the rotifers wasmaintained by using a computer to control the concentrationof algae, as rotifer food, in the rotifer culture. As rotifersconsumed algae, the turbidity decreased until a set-point wasreached. Then fresh algal suspension (supplied from a steady-statealgal chemostat) was metered into the rotifer culture, whichwas held in the dark. Rotifer and algal populations, as wellas rotifer µ^max entered steady states. These steady-stateresults were consistent with previous data from chemostat studies,but growth transients indicated that the of the µ^maxrotifersmay be subject to selection. The system is unique in providinga means to explore population dynamics of a metazoan maintainednear its µ^max.     

Effect of high light on the efficiency of photochemical energy conversion in a variety of lichen species with green and blue-green phycobionts

Exposure to high light induced a quantitatively similar decrease in the rate of photosynthesis at limiting photon flux density (PFD) and of photosystem II (PSII) photochemical efficiency, F_V/F_M, in both green and blue-green algal lichens which were fully hydrated. Such depressions in the efficiency of photochemical energy conversion were generally reversible in green algal lichens but rather sustained in blue-green algal lichens. This greater susceptibility of blue-green algal lichens to sustained photoinhibition was not related to differences in the capacity to utilize light in photosynthesis, since the light-and CO₂-saturated rates of photosynthetic O₂ evolution were similar in the two groups. These reductions of PSII photochemical efficiency were, however, largely prevented in lichen thalli which were fully desiccated prior to exposure to high PFD. Thalli of green algal lichens which were allowed to desiccate during the exposure to high light exhibited similar recovery kinetics to those which were kept fully hydrated, whereas bluegreen algal lichens which became desiccated during a similar exposure exhibited greatly accelerated recovery compared to those which were kept fully hydrated. Thus, green algal lichens were able to recover from exposure to excessive PFDs when thalli were in either the hydrated or desiccated state during such an exposure, whereas in blue-green algal lichens the decrease in photochemical efficiency was reversible in thalli illuminated in the desiccated state but rather sustained subsequent to illumination of thalli in the hydrated state.Abbreviations and Symbols F_o yield of instantaneous fluorescence - F_M maximum yield of fluorescence induced by pulses of saturating light - F_V variable yield of fluorescence - PFD photon flux density (400–700 nm) - PSII photosystem II This work was supported by the Deutsche Forschungsgeneinschaft (Forscherguppe Ökophysiologic and Sonderforschungsbereich 251 of the University of Würzburg) and the Fonds der Chemischen Industrie. W.W.A. gratefully acknowledges the support of a fellowship from the Alexander von Humboldt Foundation. We thank Professor T.G.A. Green for identifying and supplying all of the New Zealand lichen material and Professor F.-C. Czygan for advice concerning the chlorophyll analyses which were performed by Johanna Leisner.     

竹类植物叶片上八种蚜虫的形态变异分析

选取寄生于竹类植物叶片上的3科6属共8种蚜虫,即居竹舞蚜Astegopteryx bambusifoliae Takahashi、小舞蚜Astegopteryx minuta (van der Goot)、居竹坚蚜Cerataphis bambusifoliae Takahashi、林栖粉角蚜Ceratovacuna silvestrii (Takahashi)、塔毛角蚜Chaitoregma tattakana (Takahashi)、竹色蚜Melanaphis bambusae (Fullaway)、竹纵斑蚜Takecallis arundinariae (Essig)和竹梢凸唇斑蚜 Takecallis taiwanus (Takahashi),在光学显微镜下观察并测量了34个形态特征;统计比较了28个形态特征在种内的变异。通过主成分分析筛选形态特征,每两两特征对应统计作图,标出每种蚜虫的95％椭圆置信区间进行分析。结果表明,体形、腹管和触角的形态在不同的科间有较大差别,喙末端、跗节Ⅰ、跗节Ⅱ及爪的形态在科间有较高的趋同性;在种内各形态特征存在一定变异,其中喙末端 （CV=3.73%～7.59%）、跗节Ⅰ （CV=4.16%～12.05%）、跗节Ⅱ （CV=3.10%～8.39%）和爪（CV=2.60%～11.68%）的变异都很小。主成分分析筛选的第一主成分为喙末端、跗节Ⅰ、跗节Ⅱ和爪,不同的椭圆区间范围提示这些特征组合基本处于稳定的范围内。与蚜虫的取食行为和附着于植物表面相关的形态特征,如喙末端、跗节Ⅰ、跗节Ⅱ和爪等在不同类群间存在显著的相似性,暗示了寄生于竹类植物叶片的蚜虫在这些形态特征上的趋同适应。最后结合蚜虫的生物学信息,初步探讨了形态适应的机制。     

Light-induced changes in the fluorescence yield of chlorophyll a in Anacystis nidulans II. The fast changes and the effect of photosynthetic inhibitors on both the fast and slow fluorescence induction

1. The intensity dependence and spectral variations during thefast transient of chlorophyll a (Chl a) fluorescence have beenanalyzed in the blue-green alga Anacystis nidulans. (Unlikethe case of eukaryotic unicellular green or red algae, the fastfluorescence induction characteristics of the prokaryotic blue-greenalgae had not been documented before.)
2. Dark adapted cellsof Anacystis exhibit two types of fluctuationsin the fluorescenceyield when excited with bright orange light(absorbed mainlyin phycocyanin). The first kinetic patterncalled the fast (sec)fluorescence transient exhibits a characteristicoriginal levelO, intermediary hump I, a pronounced dip D, peakP and a transitorysmall decline to a quasi steady state S.After attaining S,fluorescence yield slowly rises to a maximumlevel M. From M,the decline in fluorescence yield to a terminalT level is extremelyslow as shown earlier by Papageorgiou andGovindjee (8). Ascompared with green and red algae, blue-greenalgae seem tohave a small PS decline and a very characteristicslow SM rise,with a M level much higher than the peak P.
3. A prolonged darkadaptation and relatively high intensity ofexciting illuminationare required to evoke DPS type yield fluctuationsin Anacystis.At low to moderate intensities of exciting light,the time forthe development of P depends on light doses, butfor M, thisremains constant at these intensities.
4. Fluorescence emissionwas heterogeneous during the inductionperiod in Anacystis;the P and the M levels were relativelyenriched in short-wavelengthsystem II Chi a emission as comparedto D and S levels.
5. Thefast DPS transient was found to be affected by electrontransportcofactor (methyl viologen), and inhibitors (e.g.,DCMU, NH₂OH)in a manner suggesting that these changes are mostlyrelatedto the oxido-reduction level of intermediates betweenthe twophotosystems. On the other hand, the slow SM changesin fluorescenceyield, as reported earlier (5, 15), paralleloxygen evolution.These changes were found to be resistant toa variety of electrontransport inhibitors (O-phenanthroline,HOQNO, salicylaldoxime,DCMU, NH₂OH and Antimycin a). It issuggested that, in Anacystis,even in the presence of so-calledinhibitors of cyclic electronflow, a "high energy state" isstill produced.
6. Measurementsof Chlorophyll a fluorescence and delayed lightemission inthe presence of both DCMU and NH₂OH indicate thatthe slow SMchanges are not due to the recovery of the reactioncenter IIin darkness preceeding illumination.
7. Our results, thus, suggestthat in Anacystis a net electrontransport supported oxidation-reductionstate of the quencherQ regulates only partially the developmentof the DPS transient,but the development of the slow fluorescenceyield changes seemsnot to be regulated by these reactions.It appears, from datapresented elsewhere, that the slow risein the yield resultsdue to a structural modification of thethylakoid membrane.

¹We are grateful to the National Science Foundation for financialsupport. (Received November 21, 1972; )     

Experimental parameters and a biological standard for acridine orange detection of drug-induced alterations in chromatin condensation

We investigated a number of sample-preparative parameters for use of flow cytometry to detect chromatin condensation in cells stained with acridine orange after DNA in situ is partially denatured by acid treatment. Stability and data reproducibility for both control and drug-treated ME-180 and HT-29 cells were assessed over: a range of cell concentrations in 2.56 X 10(-5) M acridine orange; 15 days of storage in fixative; various times between RNase digestion and staining; and increasing times between staining and analysis. Listmode data for red and green fluorescence were collected and mean fluorescence intensities of G1, S, and G2 subpopulations of HT-29 and ME-180 cells were computed. These were normalized to data from HeLa-S3 cells and fluorescent microspheres to control for inter-experiment variations in staining and instrumental parameters, respectively. The normalized red and green fluorescence data were used to calculate alpha 1 for G1 cells [alpha t = red fluorescence/(total fluorescence)]. Exponentially growing HeLa-S3 cells were a very consistent and reproducible biological standard to control for fixation and staining variability. Mean fluorescence intensities of control and difluoromethylornithine-treated (i.e., polyamine depleted) cells remained stable and reproducible across all tested ranges for cell concentration, storage in fixative, and time after RNase digestion. This technique can thus be used to evaluate difluoromethylornithine-induced changes in chromatin condensation of samples stored for as long as 2 weeks and analyzed all on 1 day.     

An Evidence Indicating That a Weak Orange Light Absorbed by Phycobilisomes Causes Inactivation of PSII in Cells of the Red Alga Porphyridium cruentum Grown under a Weak Red Light Preferentially Exciting Chl a

Changes in the PSII fluorescence upon shift of light qualitywere studied with the red alga Porphyridium cruentum IAM R-1and supplementarily with P. cruentum ATCC 50161, the cyanophytesSynechocystis spp. PCC6714 and PCC6803 and Synechococcus sp.NIBB1071. When Porphyridium cruentum grown under a weak redlight (PSI light) preferentially absorbed by Chl a was illuminatedwith a weak orange light (PSII light) mainly absorbed by phycobilisomes(PBS), a change of PSII fluorescence at room temperature wasinduced. The ratio of Fvm (Fm— Fo) to Fm was reduced rapidlyaccompanying the increase in Fo (T^1/2 ca. 3 min). The effectsof DCMU and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinoneindicated that the fluorescence change is induced when plastoquinonepool is highly reduced. The fluorescence change after a shortPSII light illumination was reversible; it rapidly recoveredin the dark (T ^1/2 ca. 3 min). The reversibility was graduallyreduced and disappeared after 40 h under PSII light accompanyingdecrease in PSII activity per PBS down to almost 50%. Sincethe pattern of the fluorescence change resembles that observablewhen PSII is photoinactivated, PSII light probably induces thephotoinactivation of PSII, possibly reversibly at first andirreversibly after prolonged illumination. Such a rapid fluorescencechange was insignificant in Synechocystis sp. either PCC6714or PCC6803. Only a slow and small decrease in Fvm/Fm level appearedafter prolonged PSII light illumination (the reduction of PSIIactivity per PBS was around 20%). In Porphyridium, shift fromPSII light to PSI light caused a rapid and chloramphenicol-sensitiveFvm/Fm elevation during the first 10 h while the increase inPSH activity per PBS was only 10% of that before the light shift.Then, a gradual elevation followed up to the level at the steadystate under PSI light. A similar rapid increase in Fvm/Fm wasobserved with Synechocystis PCC6714, in which the synthesisof PSII is not regulated, suggesting that a rapid increase inFvm/Fm does not reflect the acceleration of the synthesis ofPSII. Results were interpreted as that (1) PSII light causesphotoinactivation of PSII. Such a photoinactivation is markedin Prophyridium cells grown under PSI light. (2) In Porphyridium,changes in the abundance of PSII upon shift of light qualityare largely attributed to the photoinactivation of this type. (Received February 19, 1999; Accepted June 14, 1999)     

Fluorescence induction in chloroplasts isolated from the green alga Bryopsis maxima III. A fluorescence transient indicating proton gradient across the thylakoid membrane

The yield of chlorophyll a fluorescence in dark-adapted intactchloroplasts isolated from the green alga, Bryopsis maxima,showed, after the first wave of the fluorescence induction wasover, a peak labelled M₁ at about 10th sec of illumination.The time to reach M₁ during continuous illumination inverselydepended upon exciting light intensity. The appearance of thepeak M₁ was accelerated by the addition of methyl viologen aselectron acceptor and delayed in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea.KCN had no effect on the peak M₁ at a concentration where photosyntheticoxygen evolution was completely suppressed. Thus, the peak M₁appears to be related to electron transport but not to the carbonreducing cycle. Carbonylcyanide m-chlorophenylhydrazone, NH₄Cl and methylaminediminished or eliminated M₁. On the other hand, an enhancementof the fluorescence yield at M₁ was observed in the presenceof energy transfer inhibitors. Valinomycin plus KCl also increasedheight of the peak M₁. However, the combined addition of valinomycinand dinitrophenol resulted in the complete elimination of thepeak M₁. These results indicate that the fluorescence peak M₁occurring at about 10 sec of illumination is linked to a protongradient across the thylakoid membrane. (Received July 7, 1977; )