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1.
We determined as to indole-3-acetic acid (IAA), endogenous levels byliquid chromatography-mass spectrometry using 13C6-IAA, diffusible levels byfluorometric detection using indole-propionic acid, and polar transportactivityby radioactive IAA in 3-month-old tomato plants (stems, leaves or roots). TheIAA concentration in the apoplast (AP) solution was higher than those in thesymplast (SP) solution in both the upper and lower parts of stems, showing thatIAA analysis of AP solution is important. Younger leaves exported much morediffusible IAA than older leaves. The IAA concentration in the main roots wasalmost at the same level as in the stems. The results suggested that thetransport capacity of IAA is probably the limiting factor for the amount of IAAtransported in stems and the amount of polar IAA transport might be only 19% ofthe endogenous IAA amount in stems. 相似文献
2.
3.
Cuttings from 7-day-old Vigna radiata seedlings were treated for 24 h with various concentrations of coumarin and/or indole-3-butyric acid (IBA), applied either
alone or in combination, in order to stimulate adventitious root formation (ARF). The effects of treatment on endogenous free
and conjugated indole-3-acetic acid (IAA), basic peroxidase (basic PER) activity and its isoperoxidases analysis and their
relation to ARF were then investigated at the potential rooting sites during the first 96 h after application. Simultaneously,
combined treatments acted synergistically in inducing more adventitious roots in treated cuttings than in those treated with
coumarin or IBA individually, as compared with the control. Endogenous free IAA increased transiently in treated cuttings
as compared with the control and the maximum increase occurred with the combined treatment. This suggests that coumarin and
IBA may act synergistically in increasing the endogenous free IAA level during the induction phase of rooting to initiate
more roots. Likewise, higher level of conjugated IAA was also found in treated cuttings than in untreated ones, during the
primary events of ARF, with the maximum level occurring in the combined treatment. Comparison of the dynamics of conjugated
IAA and activity of basic PERs led to conclusion that the former but not the latter is responsible for downregulation of endogenous
IAA levels significantly during the primary events of ARF. A sharp increases in basic PERs occurred during the secondary events
of ARF, suggesting their role in root initiation and development rather than root induction. 相似文献
4.
The relationships between indole-3-acetic acid (IAA) diffusing out of the fruit and competition among fruits, and between fruits and shoot tips were investigated using apple ( Malus domestica Borkh. cv. Jonagold) and tomato ( Lycopersicon esculentum Mill.) plants. Dominant fruits always had more diffusible IAA than subordinated, inhibited fruits. Alterations in dominance – by fruit- or shoot tip removal – led to significant changes in diffusible IAA by the remaining fruits. This change could be detected one day after dominance modification.
It is suggested that diffusible IAA is involved in the correlative signal regulating dominance relationship between fruits, and between fruits and shoots in apple and tomato. 相似文献
It is suggested that diffusible IAA is involved in the correlative signal regulating dominance relationship between fruits, and between fruits and shoots in apple and tomato. 相似文献
5.
Andrzej Pacholczak Wiesław Szydło Aleksandra Łukaszewska 《Acta Physiologiae Plantarum》2005,27(4):417-428
In order to improve vegetative propagation of a difficult to root Cotinus coggygria the stock plants were subjected to: etiolation, shading and spraying with IBA, combined with the application of two commercially
available rooting powders. The IBA treatment was more suitable for rooting of C. coggygria cuttings than the NAA application and it enhanced rhizogenesis regardless of the form of auxin application (foliar application
to a stock plant or a rooting powder used directly on cuttings) and the amount of light provided to stock plants. Etiolation
did not improve rhizogenesis in stem cuttings, however, reduction of light intensity by 50% and 96% of the ambient prior to
harvest of cuttings affected rooting positively. Positive effects of shading can be ascribed to changes in shoot anatomy,
i.e. a weaker sclerenchyma development. Synergistic effect of shading and foliar auxin application can result from the increase
in leaf blade area and/or thinner lower epiderm. Enhanced rooting in cuttings from shoots grown out under reduced light intensity
was accompanied by decrease in the contents of total soluble sugars, soluble proteins and free ABA and by increase in total
chlorophyll, free amino acids, polyphenolic acids and free IAA contents. 相似文献
6.
Kamel Ahmed Hussein Tartoura 《Acta Physiologiae Plantarum》2001,23(2):149-156
Endogenous levels of free and conjugated IAA, auxin protectors (Prs) and peroxidase (PER) activity and their relation to adventitious
root initiation (ARI) were investigated at the potential sites of adventitious rooting in relation to exogenous application
of 250 μM ABA during the first 120 h after treatment. Cuttings from 7-day-old mung bean [Vigna radiata (L.) Wilcz.] seedlings were treated with 125, 250, and 500 μM ABA for 24 h. ABA significantly stimulated ARI but extremely
inhibited epicotyl growth as compared to control. Free and conjugated IAA were measured by reversed-phase high performance
liquid chromatography while Prs and PER activities were measured spectrophotometrically. The present results also indicate
that endogenous free IAA levels peaked later in ABA-treated cuttings than that in control, suggesting that ABA extended the
length of the induction phase of rooting process in treated cuttings and that might explain the significant delay of the appearance
of roots at the treated cuttings. Higher level of IAA conjugates was found in ABA-treated cuttings than that in untreated
ones. Pr level also peaked later in ABA-treated cuttings than that in control, indicating that ABA extended the period of
Pr activity. An initial temporary decrease of PER activity was found in associating with high levels of free IAA and Prs during
most of the primary events, while the opposite occurred during the secondary events of adventitious rooting process in both
treated and untreated cuttings. Thus, ABA may stimulate ARI in mung bean Vigna radiata cuttings by regulating the concentration and /or activities of endogenous IAA, Prs, and PER activity in favor of inducing
a large number of adventitious roots at their potential sites of adventitious rooting. 相似文献
7.
IAA和GA3在调控豌豆黄化苗茎切段伸长生长中的相互作用 总被引:4,自引:0,他引:4
IAA和GA3均能促进豌豆黄化苗茎切段的伸长。IAA效应可以为GA的合成抑制剂S-3307抑制,GA3效应同样也为IAA的运输抑制剂TIBA所抑制,并且分别再施用GA3和IAA后,抑制效应又能有所解除。观察顶端切半茎切段的结果表明,IAA主要促进茎切段表皮细胞的伸长,而GA3可能主要促进内部组织细胞的伸长。观察切段横纵切片的结果则显示,IAA促进皮层细胞的伸长和增大,而GA3只促进皮层细胞的伸长。这些结果说明两者是通过不同的作用部位和方式共同调节豌豆茎切段伸长生长的。 相似文献
8.
黄瓜果实中ARF和Aux/IAA基因对外源激素的应答 总被引:1,自引:0,他引:1
以非单性结实黄瓜自交系‘6429’为试验材料,对当天开花的果实进行CPPU、Spd、NAA、2,4-D和IAA等5种生长物质处理,以清水为对照,选取9个ARF基因(Csa019264、Csa019265、Csa009209、Csa009210、Csa021954、Csa012237、Csa012805、Csa015176、Csa010564)和5个Aux/IAA基因(Csa003118、Csa012115、Csa016715、Csa006680、Csa018571)设计特异引物,取开花当天与花后第2、4天的果实及其茎、叶作RT-PCR分析。结果显示,9个ARF基因的表达水平显示出了很大的差异,Csa012805在所有激素处理后的果实中都有较高水平的表达而清水处理中未检测到;5个Aux/IAA基因中的4个在6种处理后的果实及茎叶中都有表达,推测是组成型表达基因,Csa016715在激素处理后的果实中比清水处理的未发育果实中的表达水平高。推测Csa012805和Csa016715这2个基因对黄瓜果实膨大起促进作用。 相似文献
9.
A. A. Kotov 《Plant Growth Regulation》1996,19(1):1-5
The relationship between the amount of indole-3-acetic acid transported (IAA transport) through the second node of 7-day-old pea seedlings and the degree of inhibition of axillary bud outgrowth at the same node was studied. For both the endogenous apical IAA source (leaves of apical bud) and the exogenous one (lanolin paste containing 0.25–1.0 mg mL–1 IAA) the slope of linear dependence between inhibition and IAA transport was similar. However, the same IAA transport induced different inhibitions, which were higher for the endogenous source. Moreover, the apical bud induced higher inhibition at the same level of IAA transport when the 4th leaf was present than when it was absent. Apparently, the source of IAA also may regulate the inhibitory power of IAA transported from it. IAA transport appears to consists of active and slightly active one moving along different pathways.Abbreviations
a and b
coefficients of linear regression of the type y = a+bx;
-
confidence level of t-test
- ELISA
enzyme linked immunosorbent assay
- GR1,2
e/d
growth rate of the lateral bud of experimental/decapitated (control) pea plants at the first and second days after treatment or decapitation
- I
degree of inhibition of lateral bud outgrowth
- IAA
indole-3-acetic acid
- L1,2,3
the lengths of lateral bud at 1, 2 or 3rd day after treatment or decapitation of pea plants
- n
data number
- r
correlation coefficient
- T
amount of IAA transported through the second node of pea plant for 3 hours
- TIBA
2, 3, 5-triiodobenzoic acid
- t-test
statistical test used here to compare slopes of linear regressions (y = a+bx) calculated as % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaaeiDaiaabc% cacaqG9aGaaeiiaiaadkgadaWgaaWcbaGaaGymaaqabaGccaqGGaGa% aeylaiaabccacaWGIbWaaSbaaSqaaiaaikdaaeqaaOGaaeiiaiaab+% cacaqGGaWaaOaaaeaacaqGBbaaleqaaOGaaeikaiaabohacaqGLbGa% aeiiaiaadkgadaWgaaWcbaGaaGymaaqabaGccaqGPaWaaWbaaSqabe% aacaqGYaaaaOGaaeiiaiaabUcacaqGGaGaaeikaiaabohacaqGLbGa% aeiiaiaadkgadaWgaaWcbaGaaGOmaaqabaGccaqGPaWaaWbaaSqabe% aacaqGYaaaaOGaaeyxaiaab6caaaa!524A!\[{\text{t = }}b_1 {\text{ - }}b_2 {\text{ / }}\sqrt {\text{[}} {\text{(se }}b_1 {\text{)}}^{\text{2}} {\text{ + (se }}b_2 {\text{)}}^{\text{2}} {\text{]}}{\text{.}}\] 相似文献
10.
离体马尾松(Pinus massoniana Lamb.)胚萌发过程中,过氧化物酶活性逐步增强,10h和40h出现两个剧增期,IAA氧化酶活性逐渐降低、10h和40h出现两个剧降期,IAA含量则一直维持上升趋势,同位素前体标记检测表明,蛋白质合成速率在5h明显提高,15h速率加快,30-40h出现一个平缓阶段,40h后剧增;RNA合成速率,在15h以前处于低水平,15h开始加快,25-30h出现一个平缓阶段,30h后速度提高;DNA合成速率在40h才明显加快。 相似文献
11.
High irradiance during treatment of mung bean cuttings favours root formation in response to supplied auxin, whether the latter
is IAA or IBA. On the other hand it is inhibitory towards root formation in the absence of supplied auxin. Light promotes
the uptake of14C-IAA into cuttings and its upward movement into the leaves. When14C-IAA is applied to leaves of cuttings high irradiance favours movement of radioactivity into the epicotyl and hypocotyl.
This movement is also enhanced by concomitant supply of IBA to the base of the cuttings. The irradiance under which stock
plants are raised also affects the extent of root formation on cuttings. When cuttings are held in darkness without a supply
of exogenous auxin they root best if prepared from seedlings raised under high irradiance. However, transport of14C-IAA out of leaves of cuttings is favoured when cuttings are prepared from seedlings grown under low irradiance. These observations
are discussed in relation to auxin transport, photodestruction and, possibly, metabolism. 相似文献
12.
Changes in the Levels of IAA and ABA in Cucumber Leaves under Progressive Soil Drought 总被引:1,自引:0,他引:1
Pustovoitova T. N. Zhdanova N. E. Zholkevich V. N. 《Russian Journal of Plant Physiology》2004,51(4):513-517
Changes in the IAA and ABA contents in cucumber (Cucumis sativus L.) leaves during adaptation to drought were studied. An increase in the water-retaining capacity and heat resistance of leaves indicating the onset of adaptation occurred when the leaf growth has been already suppressed. There was a transient increase in the ABA content during the initial stage of adaptation. An increased IAA content was maintained for a longer period, throughout about two-third of the adaptation period. A second increase in the ABA content was observed before the onset of leaf permanent wilting, when IAA content already decreased. Our data suggest that not only ABA, but also IAA are involved in the development of defense responses during the adaptation to drought. 相似文献
13.
Charles Moncousin Jean-Michel Favre Thomas Gaspar 《Plant Cell, Tissue and Organ Culture》1989,19(3):235-242
Node cuttings of in vitro cultured grapevine were rooted in absence of any growth regulator, before the onset of the axillary bud. There were two peaks of ethylene production at 2 and 10–12 h, well marked in the top and bottom portions of the cuttings for the former. The level of IAA increased in the basal portions of the cuttings only, from the 4th hour, and culminated at the 24th hour. The wound ethylene of the first rise might be initiating the sequence of reactions leading to root formation. The second ethylene rise might result from the beginning of the increase of the IAA level. 相似文献
14.
甘露醇和6—BA处理对水稻细胞过氧化物酶及IAA氧化酶活性的影响 总被引:2,自引:0,他引:2
研究了甘露醇和60BA处理对水稻服浮细胞再分化、过氧化物酶及IAA氧化酶的影响。结果表明,甘露醇处理能延迟水稻细胞衰老,提高细胞再分化能力,降低细胞过氧化物酶和IAA氧化酶活性,6-BA(2mg/L)虽然明显降低细胞过氧化物酶活性,但对IAA氧化酶及细胞衰老无明显影响,讨论了过氧化物酶及IAA氧化酶在水稻胚性细胞形成上的可能作用。 相似文献
15.
目的:探讨胰岛素自身抗体(IAA)、胰岛细胞抗体(ICA)及谷氨酸脱羧酶抗体(GADA)联合检测对糖尿病分型的诊断价值。方法:选择2015年6月~2016年6月在我院进行诊治的1型糖尿病患者30例为A组,2型糖尿病患者60例为B组,同期在我院进行体检健康者50例为C组,采用酶联免疫吸附法(ELISA)检测三组的IAA、ICA及GADA,比较三组的阳性检出率。结果:A组空腹血糖为(10.12±3.68)mmol/L,B组空腹血糖为(11.23±3.26)mmol/L,A组和B组的空腹血糖均明显高于C组(4.35±1.42mmol/L)(P0.05),但A组和B组的空腹血糖相比无明显差异(P0.05);A组和B组的IAA、ICA及GADA单独和联合检测的阳性率均明显高于C组(P0.05),且A组的IAA、ICA及GADA单独和联合检测的阳性率明显高于B组(P0.05);IAA、ICA及GADA联合检测对1型和2型糖尿病的敏感性和特异性均明显高于单独检测(P0.05)。结论:IAA、ICA及GADA联合检测对糖尿病分型具有较高的临床诊断价值。 相似文献
16.
The transport of14C-IAA and14C-ABA applied exogenously to root cap toward the elongation zone was investigated in gravi- and light-stimulated primary roots
ofZea mays L. cv. Golden Cross Bantam 70. No significant difference of either IAA or ABA in radioactivities was observed between upper
and lower halves of elongation zones during the latent period (0–60 min after the stimulation) of gravitropic response. When
quantitative analysis of endogenous IAA and ABA by an internal standard method was carried out 60 min after gravi- and/or
light-stimulation, no asymmetric redistribution of either IAA or ABA was observed between upper and lower halves of elongation
zones. Light irradiation increased by 20% the contents of ABA in elongation zones. These results suggest that although both
IAA and ABA are basipetally transportable and can transmit their information to the elongation zone during a latent period
we cannot explain the gravitropic curvature by their redistributions between the two (upper and lower) halves of primary roots
ofZea. On the basis of results from the present work and previous papers, the distribution of IAA and ABA in gravistimulatedZea roots is discussed.
A part of this study was reported at the Eighth Annual Meeting of the IUPS Commission on Gravitational Physiology at Tokyo
1986. 相似文献
17.
Changes in peroxidase and IAA oxidase activities during cell elongation in Phaseolus hypocotyls 总被引:1,自引:0,他引:1
Cytoplasmic and salt-extracted peroxidase and IAA oxidase activities were studied in Phaseolus vulgaris hypocotyls treated with gibberellic acid (GA, 200 μM), naphthyl acetic acid (NAA, 100 μM) and distilled water control (DW).
Peroxidase activity was assayed with four hydrogen donors during the initial phase of hypocotyl elongation. Though peroxidase
activity showed a decreasing trend with time in all the hydrogen donors studied; considerable variation with different hydrogen
donors was observed. NAA had maximum peroxidase activity as compared to DW or GA treatment. The activity showed a clear inverse
correlation with hypocotyl growth. IAA oxidase activity showed a similar trend with growth as peroxidase activity. A highly
significant correlation was observed between peroxidase and IAA oxidase activities and high molecular weight xyloglucan content
(P<0.001). Finally, the possible role of peroxidase and IAA oxidase activities in hypocotyl elongation growth is discussed. 相似文献
18.
19.
以黄瓜‘津研四号’幼苗为试材, 采用Hoagland营养液栽培, 研究了不同浓度(0、0.01、0.1、1和10 μmol·L-1) IAA处理对50 mmol·L-1 NaHCO3胁迫下黄瓜幼苗光合特性及抗氧化系统的影响。结果表明, 碱胁迫对黄瓜幼苗的生长有抑制作用, 0.01-1 μmol·L-1外源IAA处理可显著增加黄瓜幼苗的生物量; 使叶中Na+积累降低, K+积累增加, 且IAA的缓解效果具有浓度效应。叶绿素a、叶绿素b和类胡萝卜素含量提高, 净光合速率(Pn)和气孔导度(Gs)增加, 以1 μmol·L-1 IAA处理的效果最好。添加1 μmol·L-1外源IAA显著提高了碱胁迫下黄瓜叶中超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)和谷胱甘肽还原酶(GR)的活性及还原型抗坏血酸(AsA)和谷胱甘肽(GSH)的含量, 降低了碱胁迫诱导的活性氧积累和膜脂过氧化反应; 而10 μmol·L-1外源IAA处理则加剧碱胁迫对黄瓜幼苗的危害。 相似文献