Medline (PubMed)遗传学杂志文献搜索引擎开发研究

为了方便遗传学工作者快捷、准确地查阅遗传学杂志文献,开发和利用NCBI 的 Medline数据库的遗传学杂志文献资源。从Medline (PubMed)中检索并列出173种有关遗传学的杂志名录,制作了每种杂志及部分杂志年份相应的超级链接,组成一个有关遗传学杂志文献的搜索引擎。此搜索引擎能快捷、准确、动态地检索173种遗传学杂志全部文献题录及部分杂志各年的文献题录,并可在PubMed中进一步检索出文献的摘要和部分全文。 Abstract：To help geneticists look up genetics journals more quickly and exactly,this paper developed this search engine of genetics journals. The paper exploited the search engine with genetics journals sources of Medline database in NCBI, indexed and listed 173 names of genetics journals from Medline. Then obtained one search engine concerning genetics journals after making every journal´s super linkage. The search engine can search out not only all articles´ titles of 173 genetics journals but also some articles’ titles of the journals each year quickly,exactly and variablely. We can further obtain all articles´ abstracts and some full texts in PubMed.     

分子生物学杂志文献搜索引擎开发研究

为了方便从事分子生物学研究人员快捷、准确地查阅相关杂志文献,开发和利用NCBI的Medline数据库的杂志文献资源。我们从Medline(PubMed)中检索并列出184种有关分子生物学的杂志名录,制作了每种杂志的超级链接及部分杂志年份相应的超级链接,组成一个有关分子生物学杂志文献的搜索引擎。此搜索引擎能快捷、准确、动态地检索184种分子生物学杂志全部文献题录及部分杂志各年的文献题录,并可在PubMed中进一步检索出文献的摘要和部分全文。     

生态生物化学(八):植食性昆虫对食物的选择

动物取食的习性,即对食物的选择性,是在漫长的历史进化中形成的。昆虫种类繁多,这与其食性的分化是分不开的。昆虫的食性多种多样,有植食性的,即以植物及其产物为食的;有肉食性的,即以动物为食,包括捕食性     

《生物化学原理》(第2版) (双色版)(附数字课程)

主编:杨荣武书号:978-7-04-035696-0出版时间:2012年9月定价:88.00元字数:130万本书是以南京大学生物化学教学改革成果和教材建设实践经验为基础,吸收国内外多本优秀教材的优点编写而成。     

中国生物化学与分子生物学会临床应用生物化学与分子生物学分会(筹)第一届理事会召开

2005年9月11日,在中国生物化学与分子生物学会专职副秘书长兼办公室主任王同喜高级工程师的主持下,召开了刚由参会代表选举产生的分会第一届理事会议。会议按学会分支机构组织结构原则选举产生了分会第一届理事会常务理事、副理事长、理事长和秘书长。田亚平教授当选为分会理事长;吕元、潘柏申、尚红、申子瑜当选为分会副理事长：陈祥、郭健、郝晓柯、姜傥、康熙雄、鲁辛辛、吕元、潘柏申、尚红、申子瑜、台虹、田亚平、汪德清、姚智、于晓妨、张曼、赵卫国当选为分会常务理事;汪德清任分会秘书长。于当日晚7时由新任分会理事长田亚平教授在解放军总医院健宾楼会议室继续主持召开了第一届理事会。     

生物化学杂志第二卷(1986年)总目录

曾桂超王家槐(1)(9)期述︸一一第综一DNA拓扑异构酶的研究进展······……B一DNA的深沟是蛋白质“识别螺旋”的最佳结合部位· 研究报告一次密度梯度超速离心分离人血清的VLD工J、LDL、HDL:、HDL3及无脂血清···············……王克勤何铭麟人红细胞膜带3蛋白重组入脂质体及其‘550乏一运输活性·一······4~一范培昌王灵宝吴玲赵海燕李力河蚌C一反应蛋白的分离纯化及其性质研究···································,·······················…     

生物化学杂志 第五卷(1989年)总目录

第一期研究报告人正常子宫肌肉的鞘搪脂组成及其在不同生理功能阶段的变化·····································t.·一·········……邓会山等水稠叶绿体DNA的提取和纯化···················~··,·················································.’·············……沈志伟娜3尹一末端标记法制备乙型肝炎病毒(HBV)直接重复序列(DR)的生物素化的寡核替破探针 ······……‘’.“·’‘··…     

生物化学杂志第三卷(1987年)总目录

第期蛛述斑老的生化探讨···············……胜直免度应普及杭曲肚应用前景..................……张昌板(1)张双全月资铭成正武(11)研究报告一次密度禅度超遨离心分禽人t清阅种主要幽蛋自的某些理化性质的研究···························……何二王克肠卜峨辐射对大民肝、牌染色质及转录活性染色质棋贻活性的形响·····································,.…周度金肠橄康川兮班号玻对跳肝线独体暇化确暇化柑用的实脸研究······,·······,··…     

生物化学杂志第四卷(1988年)总目录

第期综述致癌作用多阶段观点与癌荃因协同作用的协调··········,·······························································……刘培楠(l) 研究报告常山凝集素的分离纯化及其性质研究········,··········4······································,·······……郑常文肖啸王永丽(12)脊盆和周围神经可溶性酸性蛋白的研究································…     

BioText Search Engine: beyond abstract search

The BioText Search Engine is a freely available Web-based application that provides biologists with new ways to access the scientific literature. One novel feature is the ability to search and browse article figures and their captions. A grid view juxtaposes many different figures associated with the same keywords, providing new insight into the literature. An abstract/title search and list view shows at a glance many of the figures associated with each article. The interface is carefully designed according to usability principles and techniques. The search engine is a work in progress, and more functionality will be added over time. Availability: http://biosearch.berkeley.edu.     

A Real-Time All-Atom Structural Search Engine for Proteins

Protein designers use a wide variety of software tools for de novo design, yet their repertoire still lacks a fast and interactive all-atom search engine. To solve this, we have built the Suns program: a real-time, atomic search engine integrated into the PyMOL molecular visualization system. Users build atomic-level structural search queries within PyMOL and receive a stream of search results aligned to their query within a few seconds. This instant feedback cycle enables a new “designability”-inspired approach to protein design where the designer searches for and interactively incorporates native-like fragments from proven protein structures. We demonstrate the use of Suns to interactively build protein motifs, tertiary interactions, and to identify scaffolds compatible with hot-spot residues. The official web site and installer are located at http://www.degradolab.org/suns/ and the source code is hosted at https://github.com/godotgildor/Suns (PyMOL plugin, BSD license), https://github.com/Gabriel439/suns-cmd (command line client, BSD license), and https://github.com/Gabriel439/suns-search (search engine server, GPLv2 license).

This is a PLOS Computational Biology Software Article

     

In Search of Mitochondrial Mechanisms: Interfield Excursions between Cell Biology and Biochemistry

Developing models of biological mechanisms, such as those involved in respiration in cells, often requires collaborative effort drawing upon techniques developed and information generated in different disciplines. Biochemists in the early decades of the 20th century uncovered all but the most elusive chemical operations involved in cellular respiration, but were unable to align the reaction pathways with particular structures in the cell. During the period 1940–1965 cell biology was emerging as a new discipline and made distinctive contributions to understanding the role of the mitochondrion and its component parts in cellular respiration. In particular, by developing techniques for localizing enzymes or enzyme systems in specific cellular components, cell biologists provided crucial information about the organized structures in which the biochemical reactions occurred. Although the idea that biochemical operations are intimately related to and depend on cell structures was at odds with the then-dominant emphasis on systems of soluble enzymes in biochemistry, a reconceptualization of energetic processes in the 1960s and 1970s made it clear why cell structure was critical to the biochemical account. This paper examines how numerous excursions between biochemistry and cell biology contributed a new understanding of the mechanism of cellular respiration.     

The Biochemistry of Parasites

HPLC-analysis of the reaction products of a series of 4-methylumbelliferyl glycosides from cello-oligosaccharides, used as substrates of a cellobiohydrolase from Trichoderma reesei, proves the lack of specificity for terminal cellobiosyl groups. Also, different reaction patterns are observed for this CBHI and for an endocellulase, when acting on these same substrates. 4-Methylumbelliferyl β-D-lactoside is an unexpected substrate for CBHI, yielding only lactose and phenol as reaction products. The binding characteristics of p-nitrobenzyl 1-thio-β-D-lactoside for this enzyme are determined by a dia-filtration technique, yielding 1 binding site and an association constant of 4.0 × 10⁴ M^?1.     

The Biochemistry of Mitosis

In this article, we will discuss the biochemistry of mitosis in eukaryotic cells. We will focus on conserved principles that, importantly, are adapted to the biology of the organism. It is vital to bear in mind that the structural requirements for division in a rapidly dividing syncytial Drosophila embryo, for example, are markedly different from those in a unicellular yeast cell. Nevertheless, division in both systems is driven by conserved modules of antagonistic protein kinases and phosphatases, underpinned by ubiquitin-mediated proteolysis, which create molecular switches to drive each stage of division forward. These conserved control modules combine with the self-organizing properties of the subcellular architecture to meet the specific needs of the cell. Our discussion will draw on discoveries in several model systems that have been important in the long history of research on mitosis, and we will try to point out those principles that appear to apply to all cells, compared with those in which the biochemistry has been specifically adapted in a particular organism.The aim of mitosis is to separate the genome and ensure that the two daughter cells inherit an equal and identical complement of chromosomes (Yanagida 2014). To achieve this, eukaryotic cells completely reorganize their microtubules to build a mitotic spindle that pulls apart the sister chromatids after the cohesin complexes are cut (see Cheeseman 2014; Hirano 2015; Reber and Hyman 2015; Westhorpe and Straight 2015) and, subsequently, use the actin cytoskeleton to divide the cell into two (cytokinesis) (see D’Avino et al. 2015). In some cells, such as in budding and fission yeasts, the spindle is built within the nucleus (closed mitosis), whereas in others, the nuclear envelope breaks down and the condensed chromosomes are captured by microtubules in the cytoplasm (open mitosis). This difference in the spatial organization of the mitotic cell has ramifications for the machinery controlling mitosis. In particular, the breakdown of the nuclear compartment disrupts the guanosine triphosphate (GTP)–guanosine diphosphate (GDP) gradient of the small GTPase called Ran. Ran usually controls nuclear-cytoplasmic transport through the importin chaperones; Ran-GDP in the cytoplasm promotes binding to nuclear transport substrates, whereas Ran-GTP in the nucleus promotes their dissociation (Güttler and Görlich 2011). As a result of nuclear envelope breakdown (NEBD), another Ran-GTP gradient is generated around the chromosomes, to which the RCC1 GTP-exchange factor binds (Clarke 2008). This Ran-GTP gradient is important for the interaction between microtubules and chromosomes because the high Ran-GTP levels around chromosomes promote the dissociation between the importin β chaperone and its binding partners, several of which help to stabilize or nucleate microtubules (Carazo-Salas et al. 1999; Kalab et al. 1999; Gruss et al. 2001; Wilde et al. 2001; Yokoyama et al. 2008).The dramatic reorganization of the cell at mitosis must be coordinated in both time and space. There are several key temporal events: entry to mitosis, sister chromatid separation, and mitotic exit, and these are effectively made unidirectional by the biochemical machinery. We will discuss the biochemistry behind each of these temporal events, in turn, but it is important to emphasize that the control mechanisms are also spatially organized. Our understanding of this spatial organization has improved dramatically with advances in the technology to detect gradients of activity in cells, and this has revealed the importance of local gradients of antagonistic protein kinases and phosphatases, GTP-binding protein regulators, and ubiquitin ligases and deubiquitylases, to name only a few of the more prominent examples (reviewed in Pines and Hagan 2011).     

Characterizing the Time-Perspective of Nations with Search Engine Query Data

Vast quantities of data on human behavior are being created by our everyday internet usage. Building upon a recent study by Preis, Moat, Stanley, and Bishop (2012), we used search engine query data to construct measures of the time-perspective of nations, and tested these measures against per-capita gross domestic product (GDP). The results indicate that nations with higher per-capita GDP are more focused on the future and less on the past, and that when these nations do focus on the past, it is more likely to be the distant past. These results demonstrate the viability of using nation-level data to build psychological constructs.