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Zakalyukina Yuliya V. Osterman Ilya A. Wolf Jacqueline Neumann-Schaal Meina Nouioui Imen Biryukov Mikhail V. 《Antonie van Leeuwenhoek》2022,115(6):841-841
Antonie van Leeuwenhoek - 相似文献
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Zucchi TD Bonda AN Frank S Kim BY Kshetrimayum JD Goodfellow M 《Antonie van Leeuwenhoek》2012,102(1):91-98
The status of two mesophilic filamentous actinomycetes isolated from an arid Australian soil sample was determined using a polyphasic taxonomic approach. The isolates had chemical and morphological properties consistent with their classification in the genus Amycolatopsis, assignments that were supported by analysis of 16S rRNA gene sequence data. Isolate SF26T formed a distinct phyletic line and hence was sharply separated from its nearest phylogenetic neighbour, Amycolatopsis sacchari DSM 44468T. In contrast, isolate SF27T formed a subclade in the Amycolatopsis tree with Amycolatopsis vancoresmycina DSM 44592T but was separated readily from the latter by DNA:DNA pairing data. The two isolates were distinguished from one another and from their respective nearest phylogenetic neighbours using a range of phenotypic properties. These data indicate that the two isolates should be recognized as new species in the genus Amycolatopsis. The names proposed for these new taxa are Amycolatopsis bartoniae sp. nov. and Amycolatopsis bullii sp. nov. with isolates SF26T (=NCIMB 14706T = NRRL B-2846T) and SF27T (=NCIMB 14707T = NRRL B-24847T) as the respective type strains. 相似文献
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Ke Xing Wei Liu Yue-Ji Zhang Guang-Kai Bian Wen-Di Zhang Tomohiko Tamura Jung-Sook Lee Sheng Qin Ji-Hong Jiang 《Antonie van Leeuwenhoek》2013,103(2):433-439
A novel actinomycete, designated strain KLBMP 1262T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262T is most closely related to Amycolatopsis sulphurea DSM 46092T (97.96 %), Amycolatopsis ultiminotia RP-AC36T (97.50 %) and Amycolatopsis jejuensis N7-3T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C16:0, iso-C16:0 and iso-C15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262T (=KCTC 19885 T = NBRC 108679T). 相似文献
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Hairong He Xinhui Zhang Haiyan Wang Chongxi Liu Shurui Wang Junwei Zhao Jiahui Yuan Xiangjing Wang Wensheng Xiang 《Antonie van Leeuwenhoek》2014,105(6):1025-1031
A novel actinomycete, designated strain NEAU-NH11T, was isolated from muddy soil collected from a lake and characterized using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-NH11T belongs to the genus Streptosporangium, and was most closely related to Streptosporangium amethystogenes subsp. amethystogenes DSM 43179T (99.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-NH11T formed a monophyletic clade with Streptosporangium purpuratum CY-15110T (98.3 %) and Streptosporangium yunnanense CY-11007T (98.0 %), an association that was supported by a bootstrap value of 80 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from S. amethystogenes subsp. amethystogenes DSM 43179T, S. purpuratum CY-15110T and S. yunnanense CY-11007T. Moreover, strain NEAU-NH11T could also be differentiated from its closest related strains by phenotypic characteristics. Therefore, it is proposed that strain NEAU-NH11T represents a novel Streptosporangium species, Streptosporangium nanhuense sp. nov. The type strain of S. nanhuense is NEAU-NH11T. (=CGMCC 4.7131T = DSM 46674T). 相似文献
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Atika Meklat Noureddine Bouras Amar Riba Abdelghani Zitouni Florence Mathieu Manfred Rohde Peter Schumann Cathrin Spröer Hans-Peter Klenk Nasserdine Sabaou 《Antonie van Leeuwenhoek》2014,106(2):287-292
A halophilic actinomycete strain, designated H27T, was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10–15 % (w/v) and the optimum growth temperature and pH were found to be 28–37 °C and 6–7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27T were identified as MK-11 (H4) and MK-10 (H6). The major fatty acids were found to be iso-C16:0, anteiso-C17:0, 10 methyl C17:0 and 10 methyl C16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27T are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27T is most closely related to Streptomonospora alba DSM 44588T (98.8 %) and Streptomonospora flavalba DSM 45155T (98.7 %) whereas the DNA–DNA relatedness values between strain H27T and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27T should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27T (=DSM 45604T =CCUG 63369T =MTCC 11563T). 相似文献
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Xinhui Zhang Chongxi Liu Yuejing Zhang Haiyan Wang Shurui Wang Chuang Li Xiangjing Wang Wensheng Xiang 《Antonie van Leeuwenhoek》2014,105(1):237-243
A novel actinomycete, designated strain NEAU-GH7T, was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7T was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7T formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180T (99.0 %), an association that was supported by a bootstrap value of 74 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7T could also be differentiated from S. longisporum DSM 43180T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic characteristics, strain NEAU-GH7T should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliensis sp. nov. is proposed. The type strain is NEAU-GH7T (=CGMCC 4.7105T=DSM 45881T). 相似文献
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Yuejing Zhang Junwei Zhao Chongxi Liu Yue Shen Feiyu Jia Xiangjing Wang Wensheng Xiang 《Antonie van Leeuwenhoek》2014,105(1):57-64
A novel actinomycete, strain NEAU-st1T, was isolated from a soil sample collected in Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-st1T belongs to the genus Nonomuraea, being most closely related to Nonomuraea rosea GW12687T (98.91 %), Nonomuraea solani NEAU-Z6T (98.44 %), Nonomuraea rhizophila YIM67092T(98.24 %) and Nonomuraea monospora PT708T (98.02 %); similarities to sequences of other type strains of the genus Nonomuraea were lower than 98 %. Both tree-making algorithms used also supported the position that strain NEAU-st1T formed a distinct clade with its most closely related species. Morphological and physiological characteristics confirmed that the strain belongs to the genus Nonomuraea and distinguished it from its most closely related species. DNA–DNA hybridization further differentiated strain NEAU-st1T from its nearest phylogenetic neighbours. These results suggested that strain NEAU-st1T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea shaanxiensis sp. nov. is proposed. The type strain is NEAU-st1T (=CGMCC 4.7096T = DSM 45877T). 相似文献
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María del Carmen Montero-Calasanz Markus Göker Gabriele Pötter Manfred Rohde Cathrin Spröer Peter Schumann Anna A. Gorbushina Hans-Peter Klenk 《Antonie van Leeuwenhoek》2013,104(2):207-216
A novel Gram-strain positive, aerobic, actinobacterial strain, designated CF11/1T, was isolated from a sand sample obtained in the Sahara Desert, Chad. The black-pigmented isolate was aerobic and exhibited optimal growth from 25 to 35 °C at pH 6.0–8.0 and with 0–8 % (w/v) NaCl, indicating that it is a halotolerant mesophile. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The G+C content in the genome was 74.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C16:0. Analysis of 16S rRNA gene sequences showed 95.3–98.6 % pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus africanus, with the type strain CF11/1T (DSM 45422 = CCUG 62969 = MTCC 11556). 相似文献