Using diatom life-forms and ecological guilds to assess organic pollution and trophic level in rivers: a case study of rivers in south-eastern France

The European Union’s Water Framework Directive has set a target of achieving good ecological status for all aquatic environments in Europe by 2015. In order to determine the quality of aquatic environments, biological indicators such as diatoms are often used. However, biotic diatom indices can be difficult and time consuming to use because of complexity of species determination. We investigated whether the biological traits of diatoms in rivers (life-forms, size classes and ecological guilds) could be used to assess organic pollution and trophic level. We worked on a data set comprising 315 diatom species, determined at 328 river stations of south-east France and a variety of parameters. The abundances of some biological traits differed significantly between the different organic pollution and trophic levels, particularly stalked diatoms, and the motile and low-profile guilds.     

Algae as indicators of environmental change

Despite an increased awareness by governments and the general public of the need for protecting all types of aquatic habitats, human impacts continue to impair the services that these ecosystems provide. Increased monitoring activities that locus on all major biological compartments are needed to quantify the present condition of Earth's aquatic resources and to evaluate the effectiveness of regulations designed to rehabilitate damaged ecosystems. Algae are an ecologically important group in most aquatic ecosystems but are often ignored as indicators of aquatic ecosystem change. We attribute this situation both to an underappreciation of the utility of algal indicators among non-phycologists and to a lack of standardized methods for monitoring with algae.Because of their nutritional needs and their position at the base of aquatic foodwebs, algal indicators provide relatively unique information concerning ecosystem condition compared with commonly used animal indicators. Algae respond rapidly and predictably to a wide range of pollutants and, thus, provide potentially useful early warning signals of deteriorating conditions and the possible causes. Algal assemblages provide one of the few benchmarks for establishing historical water quality conditions and for characterizing the minimally impacted biological condition of many disturbed ecosystems. Preliminary comparisons suggest that algal indicators are a cost-effective monitoring tool as well.Based on available evidence from field studies, we recommend development of taxonomic indicators based on diatoms (Bacillariophyceae) as a standardized protocol for monitoring ecosystem change. Both population- and community-level indices have inherent strengths, and limitations and information from both levels of biological organization should be utilized in tandem. However, further information concerning species tolerances to a variety of anthropogenic stressors is needed if autecological indices are to be used routinely for monitoring purposes. While functional measures (e.g. productivity) may also prove useful as monitoring tools, further investigation is required to characterize the reliability of alternative methodologies and to assess the consistency of these indicators under varying field conditions.Author for correspondence     

New generation water quality guidelines for ecosystem protection

1. Water quality guidelines are important for the management of water resources. Initially, guidelines focused on quality for domestic drinking water and for agricultural, recreational and industrial purposes. More recently, the emphasis has been on ecosystem protection, as well. 2. This paper discusses the key elements of new risk-based water quality guidelines being developed in Australia and New Zealand, that should lead to more effective management and protection of aquatic ecosystems. 3. There are three essential elements to this new approach: (i) it is ecosystem-based— ideally the guidelines should be, as far as possible, ecosystem-specific; (ii) it is issue-based— the guidelines should focus on the actual issues or problems caused by physical, chemical and biological stressors rather than on the individual indicators, as at present; and (iii) it is risk-based. There is generally great difficulty in deciding whether adverse biological effects will result from various stressors added to an ecosystem. The new approach develops guideline ‘packages’ for each issue and, where possible, for each ecosystem type. Each ‘package’ consists of specified key performance indicators, trigger levels for these indicators (that is, levels which indicate the degree of risk that adverse biological effects may occur), and for high risk situations (where trigger levels are exceeded) a protocol for considering the effect of ecosystem-specific factors in reducing (or enhancing) the biological effects. 4. This paper presents a case study related to a highly relevant aquatic ecosystem issue in Australia, namely the excessive growth of cyanobacteria (blue-green algal blooms), to illustrate how the new risk-based guidelines might be applied.     

What is river health?

1. Traditionally the assessment of river water quality has been based solely on the measurement of physical, chemical and some biological characteristics. While these measurements may be efficient for regulating effluent discharges and protecting humans, they are not very useful for large-scale management of catchments or for assessing whether river ecosystems are being protected. 2. Measurements of aquatic biota, to identify structural or functional integrity of ecosystems, have recently gained acceptance for river assessment. Empirical evidence from studies of river ecosystems under stress suggests that a small group of biological ecosystem-level indicators can assess river condition. However, physical and chemical features of the environment affect these indicators, the structure and function of which may be changed by human activities. 3. The term ‘river health’, applied to the assessment of river condition, is often seen as being analogous with human health, giving many a sense of understanding. Unfortunately, the meaning of ‘river health’ remains obscure. It is not clear what aspects of river health sets of ecosystem-level indicators actually identify, nor how physical, chemical and biological characteristics may be integrated into measures rather than just observations of cause and effect. 4. Increased examination of relationships between environmental variables that affect aquatic biota, such as habitat structure, flow regime, energy sources, water quality and biotic interactions and biological condition, are required in the study of river health.     

底栖硅藻应用于河流生态系统健康评价的研究进展

硅藻是水生态系统中最重要的初级生产者之一, 不仅为生态系统中其他消费者提供碳源, 也因分布广泛、种类多、世代时间短等特点, 通常被认为是水环境及生态系统良好的指示生物。文章介绍了河流生态系统健康及生物完整性的概念, 综述了硅藻尤其是底栖硅藻在水生态系统中的自身特点及作用; 概述了硅藻在生物监测中广泛应用的前提: 对环境因子的响应和国内外对硅藻及硅藻指数在河流生态监测中的研究进展; 介绍了广泛应用的硅藻指数及基于硅藻的生物完整性指数的原理及构建方法; 指出了硅藻及硅藻指数在生物监测及评价中可能所存在的问题, 并提出了今后我国在这个领域后期需开展的工作。     

Biological responses to permafrost thaw slumping in Canadian Arctic lakes

1. Rapid environmental change occurring in high‐latitude regions has the potential to cause extensive thawing of permafrost. Retrogressive thaw slumps are a particularly spectacular form of permafrost degradation that can significantly impact lake–water chemistry; however, to date, the effects on aquatic biota have received little attention. 2. We used a diatom‐based palaeolimnological approach featuring a paired lake study design to examine the impact of thaw slumping on freshwater ecosystems in the low Arctic of western Canada. We compared biological responses in six lakes affected by permafrost degradation with six undisturbed, reference lakes. 3. Slump‐affected lakes exhibited greater biological change than the paired reference systems, although all systems have undergone ecologically significant changes over the last 200 years. Four of the six reference systems showed an increase in the relative abundance of planktonic algal taxa (diatoms and scaled chrysophytes), the earliest beginning about 1900, consistent with increased temperature trends in this region. 4. The response of sedimentary diatoms to thaw slumping was understandably variable, but primarily related to the intensity of disturbance and associated changes in aquatic habitat. Five of the slump‐affected lakes recorded increases in the abundance and diversity of periphytic diatoms at the presumed time of slump initiation, consistent with increased water clarity and subsequent development of aquatic macrophyte communities. Slump‐affected lakes generally displayed lower nutrient levels; however, in one system, thaw slumping, induced by an intense fire at the site in 1968, ostensibly led to pronounced nutrient enrichment that persists today. 5. Our results demonstrate that retrogressive thaw slumping represents an important stressor to the biological communities of lakes in the western Canadian Arctic and can result in a number of limnological changes. We also show that palaeolimnological methods are effective for inferring the timing and response of aquatic ecosystems to permafrost degradation. These findings provide the first long‐term perspective on the biological response to permafrost thaw, a stressor that will become increasingly important as northern landscapes respond to climate change.     

Morphology of Aulacoseira filaments as indicator of the aquatic environment in a large subtropical river: The Pearl River,China

To identify new indicators of aquatic environmental parameters, both qualitative (the percentages of curved filaments and filaments with ending separation spines) and quantitative morphological parameters (filament and cell size) of a dominant chain colony-forming planktonic diatom (Aulacoseira granulata) were determined in the large, subtropical Pearl River, China. The qualitative parameters mainly exhibited a spatial pattern. The percentage of curved filaments was a good bioindicator of spatial patterns because it varied along the nutrient gradient. Furthermore, the percentage of filaments with ending separation spines was a good bioindicator of seasonal variation. Although the quantitative parameters exhibited a clear temporal pattern, no single parameter could be used as a bioindicator of either spatial or temporal patterns. The link between qualitative and quantitative parameters reflected the internal adaptation mechanism of filamentous diatoms to the external environment. The methods of the present study can also be applied to ecological indicators in other aquatic ecosystems dominated by chain colony-forming diatoms.     

Phylogenetic tree of vascular plants reveals the origins of aquatic angiosperms

Although aquatic plants are discussed as a unified biological group, they are phylogenetically well dispersed across the angiosperms. In this study, we annotated the aquatic taxa on the tree of vascular plants, and extracted the topology of these aquatic lineages to construct the tree of aquatic angiosperms. We also reconstructed the ancestral areas of aquatic families. We found that aquatic angiosperms could be divided into two different categories: the four aquatic orders and the aquatic taxa in terrestrial orders. Aquatic lineages evolved early in the radiation of angiosperms, both in the orders Nymphaeales and Ceratophyllales and among basal monocots (Acorales and Alismatales). These aquatic orders do not have any extant terrestrial relatives. They originated from aquatic habitats during the Early Cretaceous. Asia would have been one of the centers for early diversification of aquatic angiosperms. The aquatic families within terrestrial orders may originate from other areas besides Asia, such as America or Australia. The lineages leading to extant angiosperms diversified early in underexploited freshwater habitats. The four extant aquatic orders were relicts of an early radiation of angiosperm in aquatic environments. Their extinct ancestors might be aquatic early angiosperms.     

宁夏沙坡头地区藻类及其分布

通过野外采样,室内培养、观察、鉴定,研究了沙坡头地区藻类多样性和分布特点,并为当地藻类资源利用提出建议。研究发现沙坡头地区藻类植物共计40种(包括1变型),其中蓝藻17种,绿藻10种,硅藻9种,裸藻4种,全为普生种,陆地生境中种类最丰富。亚气及陆地生境中蓝藻在种类数(除灌溉林地结皮)、生物量方面占主导地位;贫瘠水体生境中硅藻占主要地位,营养较丰富水体中绿藻处于优势地位。另外,陆生生境中多以具鞘微鞘藻Microcoleus vaginatus为优势种,小席藻Phormidium tenue为主要种。     

Mitosis in diatoms: rediscovering an old model for cell division

Diatoms are important protists that generate one fifth of the oxygen produced annually on earth. These aquatic organisms likely derived from a secondary endosymbiosis event, and they display peculiar genomic and structural features that reflect their chimeric origin. Diatoms were one of the first models of cell division and these early studies revealed a range of interesting features including a unique acentriolar microtubule‐organising centre. Unfortunately, almost nothing is known at the molecular level, in contrast to the advances in other experimental organisms. Recently the full genome sequences of two diatoms have been annotated and molecular tools have been developed. These resources offer new possibilities to re‐investigate the mechanisms of cell division in diatoms by recruiting information from more intensively studied organisms. A renaissance of the topic is further justified by the current interest in diatoms as a source of biofuels and for understanding massive diatom proliferation events in response to environmental stimuli.     

The fauna of athalassic saline waters in Australia and the Altiplano of South America: comparisons and historical perspectives

Similarities and differences between the fauna of inland saline waters in Australia and on the Altiplano are explored and explanations sought.Elements common to both continents include the calanoid copepod genus Boeckella (B. triarticulata in Australia, B. poopoensis and B. meteoris in South America) and the cladoceran genus Daphniopsis. Salinity data for Altiplano lakes are given for six species of Boeckella and for Daphniopsis.Ostracods have adapted to the open water of saline lakes in Australia but not in South America, a difference that may reflect past differences in the degree of predation by birds. In South America, diatoms are grazed by the flamingos Phoenicoparrus andinus and P. jamesi, while in Australia the main diatom grazer is probably the aquatic oniscoid isopod Haloniscus searlei. However, at least four species of flamingos were present in Australia during the late Cenozoic and one or more of these may well have grazed diatoms. The extinction of diatom-grazing or carnivorous flamingos, or both, in Australia may have been factors in the unique colonization of inland saline waters by H. searlei.     

Chloroplast-mitochondria cross-talk in diatoms

Diatoms are unicellular, mainly photosynthetic, eukaryotes living within elaborate silicified cell walls and believed to be responsible for around 40% of global primary productivity in the oceans. Their abundance in aquatic ecosystems is such that they have on different occasions been described as the insects, the weeds, or the cancer cells of the ocean. In contrast to higher plants and green algae which derive from a primary endosymbiosis, diatoms are now believed to originate from a serial secondary endosymbiosis involving both green and red algae and a heterotrophic exosymbiont host. As a consequence of their dynamic evolutionary history, they appear to have red algal-derived chloroplasts empowered largely by green algal proteins, working alongside mitochondria derived from the non-photosynthetic exosymbiont. This review will discuss the evidence for such an unusual assemblage of organelles in diatoms, and will present the evidence implying that it has enabled them with unorthodox metabolisms that may have contributed to their profound ecological success.     

Assessing recreational benefits as an economic indicator for an industrial harbour report card

Industrial harbours are a complex interface between environmental, economic and social systems. Trying to manage the social and economic needs of the community while maintaining the integrity of environmental ecosystems is complicated, as is the identification and evaluation of the various factors that underpin the drivers of economic, community and resource condition. An increasingly popular strategy to deal with the identification and evaluation challenges in complex human-environmental systems is to use a report card system which can be used as a summary assessment tool to monitor the health of aquatic ecosystems. To date though these have largely focused on environmental factors, and it is only very recently that attempts are being made to include social, cultural and economic indicators. There has been limited consensus in the selection of social and economic indicators applied in different aquatic report cards but as recreation is such an important activity, typically some measure of recreation benefit is included. However, there has been no commonality in the measures applied to assess its performance as an economic indicator.This paper is focused on the assessment of recreational benefits as an indicator of economic value in the report card for Gladstone Harbour in Queensland, Australia. It is the first aquatic health report card to include an assessment of the nonmarket value of recreation which makes it a more comprehensive indicator of economic value compared to other report cards based on measures of employment, participation or expenditure. There have now been three consecutive years of reporting (2014–2016) of the Gladstone Harbour report card, and the results indicate that the recreation index appears to be effective in monitoring changes over time.     

Transcriptomics responses in marine diatom Thalassiosira pseudonana exposed to the polycyclic aromatic hydrocarbon benzo[a]pyrene

Diatoms are unicellular, photosynthetic, eukaryotic algae with a ubiquitous distribution in water environments and they play an important role in the carbon cycle. Molecular or morphological changes in these species under ecological stress conditions are expected to serve as early indicators of toxicity and can point to a global impact on the entire ecosystem. Thalassiosira pseudonana, a marine diatom and the first with a fully sequenced genome has been selected as an aquatic model organism for ecotoxicological studies using molecular tools. A customized DNA microarray containing probes for the available gene sequences has been developed and tested to analyze the effects of a common pollutant, benzo(a)pyrene (BaP), at a sub-lethal concentration. This approach in diatoms has helped to elucidate pathway/metabolic processes involved in the mode of action of this pollutant, including lipid metabolism, silicon metabolism and stress response. A dose-response of BaP on diatoms has been made and the effect of this compound on the expression of selected genes was assessed by quantitative real time-PCR. Up-regulation of the long-chain acyl-CoA synthetase and the anti-apoptotic transmembrane Bax inhibitor, as well as down-regulation of silicon transporter 1 and a heat shock factor was confirmed at lower concentrations of BaP, but not the heat-shock protein 20. The study has allowed the identification of molecular biomarkers to BaP to be later on integrated into environmental monitoring for water quality assessment.     

Chemical interactions in diatoms: role of polyunsaturated aldehydes and precursors

Chemicals produced by aquatic organisms, and especially micro-organisms, have received increasing attention in the last decade for their role in shaping interactions and communities. Several cases emphasize the fact that chemical signals or defence may modulate interspecific interactions. Notably, it has been shown that diatoms, unicellular algae and key primary producers in aquatic ecosystems produce a wide range of bioactive metabolites. Among these compounds, polyunsaturated short-chain aldehydes in vitro strongly impair the reproduction of various potential grazers. In the field, the relationship between aldehyde production and reproductive failure in copepods remains unclear. Recent studies have suggested that these putative defence compounds may also be involved in intercellular communication and in interactions with competitors. Potential effects of the aldehyde precursors on various organisms have also been described. This review presents an overview of various results obtained in the last decade that could help us to understand the role of polyunsaturated aldehydes and their precursors in the ecology of diatoms. It is focused on the dichotomy between freshwater and marine environments. Indeed, most of the results on anti-proliferative aldehydes concern marine planktonic diatoms, whereas they are also known to be produced by benthic and freshwater species.     

The Application of Predictive Modelling for Determining Bio-Environmental Factors Affecting the Distribution of Blackflies (Diptera: Simuliidae) in the Gilgel Gibe Watershed in Southwest Ethiopia

Blackflies are important macroinvertebrate groups from a public health as well as ecological point of view. Determining the biological and environmental factors favouring or inhibiting the existence of blackflies could facilitate biomonitoring of rivers as well as control of disease vectors. The combined use of different predictive modelling techniques is known to improve identification of presence/absence and abundance of taxa in a given habitat. This approach enables better identification of the suitable habitat conditions or environmental constraints of a given taxon. Simuliidae larvae are important biological indicators as they are abundant in tropical aquatic ecosystems. Some of the blackfly groups are also important disease vectors in poor tropical countries. Our investigations aim to establish a combination of models able to identify the environmental factors and macroinvertebrate organisms that are favourable or inhibiting blackfly larvae existence in aquatic ecosystems. The models developed using macroinvertebrate predictors showed better performance than those based on environmental predictors. The identified environmental and macroinvertebrate parameters can be used to determine the distribution of blackflies, which in turn can help control river blindness in endemic tropical places. Through a combination of modelling techniques, a reliable method has been developed that explains environmental and biological relationships with the target organism, and, thus, can serve as a decision support tool for ecological management strategies.     

Uncertainties in biotic indicators and a corrigendum to : Implications for biomonitoring

Many bioindicators based on species composition have been proposed during the past 30 years in both terrestrial and freshwater ecosystems. Kelly (2011) raised the need to better communicate the usefulness and meaning of the indices and their uncertainties. A survey of European bioindicators of freshwater ecosystems has recently highlighted that most indices have not even considered uncertainties. While recording errors have sometimes been taken into account into the calculation of index uncertainties, the reliability of biotic indices in terms of causality and predictability between a given environmental pressure and bioindicator response have seldom been considered. This has led to serious misunderstanding of what bioindicators may be able to achieve. Here a correction to Ponader et al. (2007) is presented showing that the tolerance (ecological niche) of individual species of diatoms along nutrient enrichment gradients is much wider than formerly presented. The correction, together with the comments by Kelly (2011) and recent whole ecosystem experiments seriously question the reliability of diatoms as nutrient indicators, similarly to recent findings with aquatic macrophytes. A more mechanistic basis for biomonitoring is needed based on current ecological science, rather than early 20th century community ecology.     

The use of periphytic diatoms as a means of assessing impacts of point source inorganic nutrient pollution in south-eastern Australia

1. Periphytic diatoms are used as indicators of water quality because their ecological tolerances or preferences to environmental variables are thought to be predictable. However, much of the present autecological information for periphytic diatoms has been derived from studies conducted in the northern hemisphere. In this present study we used periphytic diatoms to determine the impacts of inorganic nutrient pollution in a tidal river system in the temperate latitudes of south‐east Australia. In so doing, we assess the suitability of the use of the ‘northern hemisphere’ ecological tolerance/preference data for periphytic diatoms. 2. Artificial substrates were used to collect periphytic diatoms at 35 sites, which were positioned along the riverbanks and the middle of the river at various distances upstream and downstream of the sewage outfall. The sampling design took into account tidal excursions and the observed sewage plume dynamics. Periphytic diatoms were collected during the austral winter month of August and the austral spring months of September and October. We deployed the artificial substrates for 4 weeks to allow the periphytic diatoms to recruit and colonise, before identifying and enumerating the assemblages. 3. Data analysis included two approaches: multivariate visualisations of combinations of environmental and biological data to investigate shifts in species structure of the periphytic diatom assemblage and multimetric indices based on ecological tolerance/preference data. 4. We found that the spatial patterns inferred from multivariate and multimetric analyses were consistent. Temporal variation in the composition of the periphytic diatom assemblage was greater than the spatial variation along horizontal sections of the river (in any one deployment) due mainly to shifts between winter and spring species. 5. Outfall effects were most apparent in winter, possibly because subsequent deployments were swamped by growth of spring periphytic diatoms. The outfall effects included a shift towards pollutant tolerant species and a reduction in the variability of the periphytic diatom assemblage across the river. 6. We conclude that the use of periphytic diatoms and associated ecological tolerance/preference data as a means of assessing impacts of point source inorganic nutrient pollution is effective. An understanding of river and sewage flow patterns is essential to the design of appropriate monitoring programmes and to the interpretation of results, especially as periphytic diatoms are sensitive to many environmental variables.     

Are alien fish a reliable indicator of river health?

1. The ability of many introduced fish species to thrive in degraded aquatic habitats and their potential to impact on aquatic ecosystem structure and function suggest that introduced fish may represent both a symptom and a cause of decline in river health and the integrity of native aquatic communities. 2. The varying sensitivities of many commonly introduced fish species to degraded stream conditions, the mechanism and reason for their introduction and the differential susceptibility of local stream habitats to invasion because of the environmental and biological characteristics of the receiving water body, are all confounding factors that may obscure the interpretation of patterns of introduced fish species distribution and abundance and therefore their reliability as indicators of river health. 3. In the present study, we address the question of whether alien fish (i.e. those species introduced from other countries) are a reliable indicator of the health of streams and rivers in south‐eastern Queensland, Australia. We examine the relationships of alien fish species distributions and indices of abundance and biomass with the natural environmental features, the biotic characteristics of the local native fish assemblages and indicators of anthropogenic disturbance at a large number of sites subject to varying sources and intensities of human impact. 4. Alien fish species were found to be widespread and often abundant in south‐eastern Queensland rivers and streams, and the five species collected were considered to be relatively tolerant to river degradation, making them good candidate indicators of river health. Variation in alien species indices was unrelated to the size of the study sites, the sampling effort expended or natural environmental gradients. The biological resistance of the native fish fauna was not concluded to be an important factor mediating invasion success by alien species. Variation in alien fish indices was, however, strongly related to indicators of disturbance intensity describing local in‐stream habitat and riparian degradation, water quality and surrounding land use, particularly the amount of urban development in the catchment. 5. Potential confounding factors that may influence the likelihood of introduction and successful establishment of an alien species and the implications of these factors for river bioassessment are discussed. We conclude that the potentially strong impact that many alien fish species can have on the biological integrity of natural aquatic ecosystems, together with their potential to be used as an initial basis to find out other forms of human disturbance impacts, suggest that some alien species (particularly species from the family Poeciliidae) can represent a reliable ‘first cut’ indicator of river health.