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1.
Irradiation of the adult grain mite,Acarus siro L., with substerilizing doses of fast electrons significantly reduced fecundity and egg viability. The number of spermatophores found in the bursa of treated females was also considerably reduced. However, no change in the shape of the spermatophores was observed. It is not known whether the decrease in fecundity and egg viability was the result of the drop in spermatophore production or to the death of sperm cells.Fast electrons had less effect upon, fecundity than the same dose of gamma radiation. However, irradiation by fast electrons had a similar effect on egg viability (expressed in percent), number of spermatophores produced per individual, and the number of adult F1 progeny.Fast-electron doses above 0.4 kGy caused sterility ofAcarus siro. 相似文献
2.
Pupae of the Queensland fruit fly or Q‐fly Bactrocera tryoni (Froggatt) are irradiated routinely to induce reproductive sterility in adults for use in sterile insect technique programmes. Previous studies suggest that adult sexual performance and survival under nutritional and crowding stress are compromised by the current target dose of radiation for sterilization (70–75 Gy), and that improved mating propensity and survival under stress by irradiated males may be achieved by reducing the target sterilization dose without reducing the level of induced sterility. This raises the question of the amount by which the irradiation dose can be reduced before residual fertility becomes unacceptable. The present study measures the levels of residual fertility in male and female irradiated Q‐flies at different irradiation doses (20, 30, 40, 50, 60 and 70 Gy), and investigates the possibility that fecundity and fertility increase between 10–15 and 30–35 days post emergence. Male flies require a higher dose than females to induce sterility, with no residual fertility found in females irradiated at doses of 50 Gy or above, and no residual fertility found in males irradiated at doses of 60 Gy or above. Irradiated females are more fecund at 30–35 days post emergence than at 10–15 days. However, fertility does not increase between 10 and 15 days post emergence and 30–35 days, even at doses below 50 Gy. The present study shows that there is scope to reduce the target sterilization dose for Q‐flies below that of the current dose range (70–75 Gy) at the same time as retaining an adequate safety margin above radiation doses at which residual fertility can be expected. 相似文献
3.
Mating competitiveness and sterility induction into cohorts of wild Anastrepha ludens (Loew) (Diptera: Tephritidae) was compared among wild and laboratory flies reared for use in the sterile insect technique Mexican program. Laboratory flies stemming from an 11-yr-old bisexual strain were either not irradiated, irradiated at 3 krad (low dose), or irradiated at 8 krad. In 30 by 30 by 30-cm Plexiglas cages, where a cohort of laboratory flies (male and female) irradiated at different doses (0, 3, and 8 krad) was introduced with a cohort of wild flies, males and females of each type mated randomly among themselves. Compared with nonirradiated laboratory and wild males, irradiated males, irrespective of dose (3 or 8 krad), induced shorter refractory periods and greater mating frequency in wild females. Nevertheless, laboratory flies irradiated at a low dose induced greater sterility into cohorts of wild flies than laboratory flies irradiated at a high dose. In a 3 by 3 by 3-m walk-in cage, wild males gained significantly more matings with wild females than nonirradiated and irradiated laboratory males a finding that revealed a strong effect of strain on mating performance. Mating incompatibility of the laboratory strain might have obscured the effect of reduced irradiation doses on male mating performance in the walk-in cage. Our results highlight an urgent need to replace the A. ludens strain currently used by the Mexican fruit fly eradication campaign and at least suggest that reducing irradiation doses result in an increase in sterility induction in wild populations. 相似文献
4.
The sterile insect technique relies on sterilization of males using ionizing radiation. Life cycle stage, and the environmental conditions under which irradiation is carried out are crucial to the provision of good‐quality insects. To identify an optimal radiation strategy for Glossina pallidipes Austen, 1903, 13‐day‐old males were irradiated at different doses in a nitrogen atmosphere. The following day the males were mated with 8‐day‐old virgin females. Pupal production of mated females was monitored for 6 weeks, and induced sterility was determined by probit analysis. Survival of the males that mated was also monitored. At least 95% sterility of irradiated males was achieved with a 158 Gy dose in nitrogen and a 125 Gy in air. Irradiation significantly lowered the probability of survival between 30 and 100 days of age (especially flies irradiated in air), but probabilities of survival were similar outside this period for irradiated and unirradiated flies. Exposure of 2‐ or 13‐day‐old males to sterilizing radiation induced similar levels of sterility in both air and nitrogen. 相似文献
5.
The effects of gamma-irradiation on migration and survival of Schistosoma mansoni schistosomula in mice 总被引:1,自引:0,他引:1
Infections with irradiated Schistosoma mansoni were established by intramuscular (i.m.) injection of mechanically transformed schistosomula. A dose of 2.3 krad. allowed persistence of a small proportion of worms to adulthood, and of these survivors the majority of the female worms were sexually sterile. However, a small proportion of 2.3 krad.-irradiated females and a larger proportion of similarly irradiated males were capable of pairing successfully with non-irradiated partners. Radiation in the range 2.3 to 10 krad. resulted in slightly reduced peak recoveries from the lungs while 20 krad. resulted in a much reduced and 40 krad. a virtual absence of survival to the lung stage. Increasing doses of radiation in the range 2.3 to 10 krad. resulted in successively fewer parasites reaching the liver. Thus, the major sites of the radiation-induced mortality appeared to be as follows: 2.3 krad., mainly in the liver; 4 krad., in the lungs and liver; 10 krad., mainly in the lungs; 20 krad., at the injection site and in the lungs and 40 krad., mainly at the injection site. The infections studied here showed reduced survival following exposure to high doses of radiation compared with the infections, established as percutaneously applied cercariae, which have been reported by other workers. Possible reasons for the disparity are discussed. 相似文献
6.
Osikowski A 《Folia biologica》2007,55(3-4):109-114
The goal of this study was to test if sperm transport to the spermathecae in the Alpine newt (Triturus alpestris) requires active co-operation of the female. Artificial insemination of anaesthetised female newts was conducted using spermatophores collected from courting males and with sperm duct contents collected from sacrificed males. Sperm was present in the spermathecae of 9 out of 10 females inseminated with the spermatophores but in only 1 out of 8 females inseminated with sperm duct contents. The females of both groups laid some eggs after insemination, and a portion of these eggs in group of females inseminated with spermatophores were fertilized. However, the number of eggs produced by the females was much lower than typical egg-production in newts. The presence of sperm in the spermathecae of females inseminated with spermatophores and lack of sperm in the spermathecae of females inseminated with sperm duct contents suggests that sperm transport is either induced by the substances present in spermatophores and/or that sperm from the sperm duct is not fully mobile in comparison with sperm from the spermatophores. 相似文献
7.
Irradiation doses currently applied to sterilize Mexican fruit flies, Anastrepha ludens (Loew) (Diptera: Tephritidae), for release under the sterile insect technique eradication campaign in Mexico, were reviewed in an effort to increase sterile male performance in the field. A dose maximizing sterility induction into wild populations was sought by balancing somatic fitness with genetic sterility. Doses of 40, 60, and 80 Gy induced 95% or more sterility in all males, which in turn induced similar degrees of sterility into a cohort of wild flies in the laboratory. However, a low dose of 40 Gy was sufficient to completely suppress egg production in females. Similarly, a mild carryover of genetic damage might have been transferred to the F1 progeny of males irradiated at 40 Gy crossed with fertile wild females. Our results suggest that the 80-Gy dose currently applied in Mexico can be lowered substantially without jeopardizing program goals. This view could be strengthened by comparing performance of males irradiated at different doses under more natural settings. In general, we discuss the value of determining irradiation doses for pest species where females are more radiosensitive than males, by selecting the dose that causes 100% sterility in females. 相似文献
8.
Males of the sorghum plant bug, Stenotus rubrovittatus (Matsumura) (Heteroptera: Miridae), transfer a spermatophore to females during copulation. After a 1‐day interval between the first and second copulation, males transferred both sperm and a spermatophore to females during the second copulation. However, when male mating interval was <1 h, they transferred sperm but no spermatophores to females during the second copulation. Therefore, the male mating interval probably produces two types of mated females, those with and those without a spermatophore. Mated females of S. rubrovittatus do not remate for at least 3 days after mating, even when courted, and lay more eggs than virgin females at the beginning of the oviposition period. The effects of spermatophores on female sexual receptivity and fecundity were examined using mated females with or without a spermatophore. Only one of the 40 (2.5%) mated females with a spermatophore remated, whereas 10 of the 26 (38.5%) without a spermatophore remated. Furthermore, mated females with a spermatophore laid more eggs than those without a spermatophore. These results suggest that spermatophores participate in reducing female sexual receptivity and enhancing female fecundity in S. rubrovittatus. 相似文献
9.
False codling moth, Cryptophlebia leucotreta (Meyrick), male and female mature pupae and newly emerged adults were treated with increasing doses of gamma radiation and either inbred or out-crossed with fertile counterparts. For newly emerged adults, there was no significant relationship between dose of radiation and insect fecundity when untreated females were mated to treated males (N female by T male). However, fecundity of treated females mated to either untreated (T female by N male) or treated males (T female by T male) declined as the dose of radiation increased. A similar trend was observed when mature pupae were treated. The dose at which 100% sterility was achieved in treated females mated to untreated males (T female by N male) for both adults and pupae was 200 Gy. In contrast, newly emerged adult males treated with 350 Gy still had a residual fertility of 5.2% when mated to untreated females, and newly emerged adult males that were treated as pupae had a residual fertility of 3.3%. Inherited effects resulting from irradiation of parental (P1) males with selected doses of radiation were recorded for the F1 generation. Decreased F1 fecundity and fertility, increased F1 mortality during development, and a significant shift in the F1 sex ratio in favor of males was observed when increasing doses of radiation were applied to the P1 males. 相似文献
10.
Soopaya R Stringer LD Woods B Stephens AE Butler RC Lacey I Kaur A Suckling DM 《Journal of economic entomology》2011,104(6):1999-2008
The radiation biology of two geographically isolated populations of the light brown apple moth [Epiphyas postvittana (Walker)] was studied in Australia and New Zealand as an initiation of a SIT/F1 sterility program. Pharate and < or = 2 d pre-emergence pupae were exposed to increasing radiation doses up to a maximum dose of 300 Gy. Fertility and other life history parameters were measured in emerging adults (parental) and their progeny (F1-F3 adults). Parental fecundity was significantly affected by increasing irradiation dose in pharate pupae only. For both populations, parental egg fertility declined with increasing radiation. This was most pronounced for the irradiated parental females whose fertility declined at a higher rate than of irradiated males. At 250 Gy, females < or = 2 d preemergence pupae produced few larvae and no adults at F1. No larvae hatched from 250 Gy-irradiated female pharate pupae. At 300 Gy, males still had residual fertility of 2-5.5%, with pharate pupae being the more radio-sensitive. Radiation-induced deleterious inherited effects in offspring from irradiated males were expressed as increased developmental time in F1 larvae, a reduction in percent F1 female survival, decreased adult emergence and increased cumulative mortality over subsequent generations. Males irradiated at > or = 150 Gy produced few but highly sterile offspring at F1 and mortality was > 99% by F2 egg. 相似文献
11.
Amblyomma americanum males were treated with 0.5, 1, 2, 3, 4, 8, and 16 krad of gamma radiation. Testes of ticks treated with 2, 3, 4, 8, and 16 krad were smaller than those of ticks irradiated at lower levels and controls. No recognizable alteration in timing of spermatogenesis was noted among the different radiation groups, but severe breakdown and depletion of germinal cells was noted at 4, 8, and 16 krad. Percent hatch of larvae from crosses of irradiated males and untreated females decreased with increasing radiation level. No hatch was observed from eggs of females mated to males treated at 2 krad or higher. 相似文献
12.
Knight AL 《Environmental entomology》2007,36(1):157-164
Studies were conducted with codling moth, Cydia pomonella L., to evaluate the mating status of male and female moths in apple, Malus domestica (Borkhausen), orchards treated with and without sex pheromone dispensers. Laboratory studies first examined the effect of multiple mating of male and female moths on female fecundity and egg fertility. Females that had mated three times had a significantly higher fecundity than singly mated moths. Sequential mating by male moths had no effect on the fecundity of female moths or egg fertility. However, male moth age did impact female fecundity, with significantly fewer eggs laid after mating with virgin 1- versus 3-d-old males. The mean size of the first spermatophore transferred by males was significantly larger than all subsequent spermatophores. Classifying spermatophores based on size was used in field sampling to categorize the mating status of the female's partner. The proportion of mated females with small spermatophores (partner had previously mated) was significantly higher in treated versus untreated orchards. The proportion of female moths caught in traps baited with pear ester that were virgin was low (相似文献
13.
Follett PA 《Journal of economic entomology》2008,101(3):710-715
The effects of irradiation on egg, larval, and pupal development, and adult reproduction in Mexican leafroller, Amorbia emigratella Busck (Lepidoptera: Tortricidae), were examined. Eggs, neonates, early instars, late instars, early pupae, and late pupae were irradiated at target doses of 60, 90, 120, or 150 Gy, or they were left untreated as controls in replicated factorial experiments. Survival to the adult stage was recorded. Tolerance to radiation increased with increasing age and developmental stage. A radiation dose of 90 Gy applied to neonates and early instars prevented adult emergence. A dose of 150 Gy was not sufficient to prevent adult emergence in late instars or pupae. The effect of irradiation on sterility was examined in late pupae and adult moths. For progeny produced by insects treated as late pupae, a total of three out of 3,130 eggs hatched at 90 Gy, 0 out of 2,900 eggs hatched at 120 Gy, and 0 out of 1,700 eggs hatched at 150 Gy. From regression analysis, the dose predicted to prevent egg hatch from the progeny of irradiated late pupae was 120 Gy, with a 95% confidence interval of 101-149 Gy. The late pupa is the most radiotolerant stage likely to occur with exported commodities; therefore, a minimum absorbed radiation dose of 149 Gy (nominally 150 Gy) has potential as a quarantine treatment. Reciprocal crosses between irradiated and unirradiated moths demonstrated that males were more radiotolerant than females. Irradiation of female moths at a target dose of 90 Gy before pairing and mating with irradiated or unirradiated males resulted in no viable eggs, whereas irradiated males paired with unirradiated females produced viable eggs at 90 and 150 Gy. 相似文献
14.
M Menon 《International journal of radiation biology and related studies in physics, chemistry, and medicine》1978,33(6):541-549
Mature males of Nauphoeta cinerea produce a sex pheromone 'seducin' which has short-range effects in attracting mature females of the same species. Exposure of newly-emerged adult males to 3.5, 7, 14 or 21 krad of gamma-radiation decreased their life expectancy and affected their mating behaviour. Bioassay of dichloromethane extracts of males showed that radiation doses (14 krad) sufficient to induce sterility did not affect the ability to produce pheromone but significantly reduced the release of pheromone by inhibiting wing-raising. The sterile-male technique using males sterilized by ionizing radiation in air may not be the method of choice for control of Nauphoeta cinerea. 相似文献
15.
William D. Brown 《Journal of Insect Behavior》2008,21(3):130-142
Previous studies on tree crickets have demonstrated female choice of males based on size and courtship feeding but less is
known about sexual selection under conditions of direct mating competition. I studied courtship, aggression and mating of
the black-horned tree cricket Oecanthus nigricornis (Walker) to test size-related sexual selection under conditions of direct sexual competition. Results show that larger individuals
of both sexes mated more frequently than their smaller counterparts, and this was due to the ability of large individuals
to out compete rivals. Large males achieved the advantage by aggressively reducing courtship by small males, whereas large
females responded to male courtship more quickly but with little aggression. Although there was no evidence here for mate
choice, there were advantages for having larger mates; fecundity increased with female size and spermatophores (which females
consume after mating) increased with male size. Size of the specialized metanotal courtship gift, however, was not related
to male size. 相似文献
16.
Ilango K 《Journal of biosciences》2005,30(5):733-747
The spermathecal complex ofPhlebotomus papatasi Scopoli (Diptera: Psychodidae) undergoes histological and physiological changes during its gonotropic cycle. The present
histochemical study revealed a mucopolysaccharide secretory mass in the spermathecae of the newly emerged sandfly. Sperm competition
occurs when two or more males compete to fertilize an ovum in the female reproductive tract. In this study, spermatophores
of two or more competing males were deposited at the base of the spermathecal ducts, which originate from the female bursa
copulatrix. This suggests that females play a role in sperm displacement, which is defined as any situation in which the last
male to mate with a female fertilizes maximum number her eggs. A blood meal ingested by the female for ovary development and
egg laying stimulates the release of sperm from the spermatophore. The spermatozoa then migrate to the lumen of the spermatheca.
The ultrastructure of spermatozoa comprises a head with double-layered acrosomal perforatorium, an elongate nucleus, and the
axoneme with a 9 + 9 + 0 flagellar pattern. This axomene differs from the aflagellate axoneme of other Psychodinae. Morphological
changes, such as the casting off of the acrosomal membrane, and histological changes in the spermatophore are also described.
Mating plugs that have been described previously in sandflies appear to be artefacts. Females ofP. papatasi may be inseminated more than once during each gonotrophic cycle, and additional inseminations may be necessary for each cycle.
The relationships between the volumes of the sperm and the spermatheca were calculated to determine sperm utilization and
fecundity ofP. papatasi. As the females ofP. papatasi mate polyandrously, the anatomical and physiological complexity of the spermathecal complex may be related to post-copulatory
sexual selection. 相似文献
17.
Lloyd D. Stringer Nicola J. Sullivan Thomas E.S. Sullivan Vanessa J. Mitchell Lee‐Anne M. Manning Flore Mas Rebecca Clare Hood‐Nowotny David Maxwell Suckling 《Entomologia Experimentalis et Applicata》2013,148(3):203-212
The sterile insect technique (SIT) potentially provides a socially acceptable approach for insect eradication of new pest incursions. The light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), was discovered in Berkeley (CA, USA) in 2006, leading to an incursion response that included this technology. In this study, we assessed factors affecting mating success from a bisex release of irradiated moths: effects of radiation dose on male multiple mating, male flight competition, female sex pheromone titre and attractiveness of irradiated females to males, and identification of successful mating in vineyards of either irradiated or wild males (identified by isotope analysis of spermatophores from sentinel females). There was a significant negative relationship between male radiation dose and mating frequency. In head‐to‐head flights of irradiated males against non‐irradiated males to a pheromone lure in a wind tunnel, irradiated males reached the lure first only 31% of the time. With increasing radiation dose, the production of the major sex pheromone component in females, (E)‐11‐tetradecenyl acetate, dropped, from 0.7 ± 0.1 ng per female in non‐irradiated females to 0.2 ± 0.07 ng per female when irradiated at 300 Gy. Male catch was reduced to 11% of control females in traps containing females irradiated at 300 Gy. Isotope analysis of spermatophores found in the bursa copulatrix of females indicated that mating success of irradiated males inside the live (entry‐only) traps containing virgin females was lower (13.1 ± 3.3%) than suggested by male catch (21.2 ± 3.8%) in pheromone traps, the current standard for assessing field competitiveness. Impacts of irradiation on male and female moth fitness should be taken into account to improve estimates of irradiated to wild male E. postvittana overflooding ratios needed for population suppression. 相似文献
18.
Wee SL Suckling DM Burnip GM Hackett J Barrington A Pedley R 《Journal of economic entomology》2005,98(3):732-738
The effects of substerilizing doses of gamma radiation on the longevity and level of inherited sterility in the Australian moth Teia anartoides Walker were determined. Six day-old male pupae were treated with 0, 100, and 160 Gy of gamma radiation by using a 1.25 MeV Cobalt60 irradiation source. Laboratory studies of male longevity showed that radiation had little impact in adult moths of the P1, F1, and F2 generations. Inherited deleterious effects resulting from irradiation were observed in the progeny of F1 and F2 generations. Outcrosses between substerile parental males or their highly sterile male progeny to wild-type females did not affect female fecundity. However, adverse effects were observed for these crosses in the rates of successful egg hatch and postembryonic development. Fertility was always greater in out-crosses involving a P1 male than in any of the F1 out-crosses. F1 males were always more sterile than F1 females, and the level of sterility for the F1 and F2 generations was higher than that of the controls. The incidence of larval and pupal mortality was higher in the F2 than the F1 generation. A dose of 100 Gy had the highest success in inducing deleterious effects that were inherited through to the F2 generation. Our results indicated that the use of partially sterilizing doses of radiation has good potential as a selective strategy for management or eradication of T. anartoides. 相似文献
19.
ERIC A. KWEI 《Zoological Journal of the Linnean Society》1978,64(2):151-175
Blue crabs Callinectes latimanus (Rath.) trapped from two lagoons were sexed, measured and weighed. The eggs of the mature females were counted to determine fecundity, and the spermatophores of the males were counted to determine the size of sexual maturity. Sizes at maturity of both male and female crabs were determined. The methods employed and the results are discussed. 相似文献
20.
ABSTRACT We determined whether female deer ticks Ixodes dammini Spielman, Clifford, Piesman & Corwin (Acari: Ixodidae) can be inseminated repeatedly and whether sperm from first or second matings take precedence in fertilizing eggs. Such information is essential to the design of attempts to reduce the fertility of these vectors of Lyme disease. Although spermatophores are present in about half of questing female ticks, they are present in virtually all those found on deer; the abundance of males on deer exceeds that of females and copulation is common. Females must be inseminated before commencing the rapid engorgement phase of feeding. Males need not be in attendance during feeding, provided that the female has been inseminated preprandially. Thus, preprandial insemination suffices to stimulate rapid engorgement, but less blood is taken than when the female is perprandially inseminated. Both types of insemination effectively fertilize eggs. Eggs from females sequentially inseminated by irradiated and non irradiated males, were fertilized mainly by sperm from the last male. Cobalt-irradiated males mate effectively and their sperm compete with those of non-irradiated males. Sperm from the second two sequential inseminations fertilize most of the eggs. By infesting deer with such irradiated male I.dammini , the abundance of these vector ticks may effectively be reduced. 相似文献