Diversity of coevolved weeds in smallholder maize fields of Mexico and Zimbabwe

Theory and empirical data suggest the areas of origin of a crop to be the general area of origin of its coevolved weeds. These longer evolved weeds would have an advantage over species with a shorter evolutionary time and migrate more successfully. We seek to identify patterns by comparing two regions with a shared crop, similar physiographic traits, but little direct contact, one of which is the area of origin of the crop. We compared the diversity of the maize weed flora and its edible components between two rural villages each of Oaxaca, Mexico, and Honde Valley, Zimbabwe, using vegetation sampling, interviews and participatory observation. The Mexican fields had higher species richness and diversity than the Zimbabwean ones. Species richness and densities were higher in the villages that receive more rainfall. Mexican fields had a mainly native weed flora with almost 80% American species and very few of African origin, whereas Zimbabwe had 32% of American and 50% of African origin. The regions shared seven American species and one of African origin. American/Mesoamerican agrestal weeds appear to be more successful in maize. Subsistence farmers in both study areas consumed about 19 edible weed species of which four were common to all villages. Our results also suggest that the presence of 3–4 species of edible weeds per field may be a general pattern in the maize-based systems, and that people not necessarily want or need more, so usefulness—at least as an edible plant—would have a limited influence on migration success.     

Small ruminant production in smallholder and pastoral/extensive farming systems in Kenya

A survey was conducted by way of personal interviews with 562 respondents comprising 459 farmers and 103 butchers/traders in selected districts in the central and western parts of Kenya, consisting of three predominantly smallholder and four predominantly pastoral/extensive districts. The study aimed to provide a better understanding of smallholder and pastoral/extensive sheep and goat farming systems in the tropics, by taking Kenya as an example. The results showed that 58% of the pastoral/extensive farmers and 46% of the smallholders indicated livestock as their main activity. Small ruminants ranked closely behind cattle in their importance. Thirty-four percent of the households kept only sheep, 18% only goats and 48% both species. The survey demonstrated the relative importance to the farmers of tangible benefits of farming sheep and goats (e.g., regular cash income, meat, manure and, in the case of goats, milk) versus intangible benefits (e.g., the role of small ruminants as an insurance against emergencies). Regular cash income and an insurance against emergencies were the highest priorities. Seventy-eight percent of the farmers reported animal sales over the previous 12 months of the survey date. Of these sales, the income was spent on school fees (32%), purchase of food (22%), farm investment (18%), medical expenses (10%), off-farm investment (9%), social activities (5%) and re-stocking (4%). Indigenous genotypes were predominant among the pastoralists and mixed crosses predominant among the smallholders. A range of traits: growth rate, body size, body shape, drought tolerance, meat quality, fertility, disease and heat tolerance, prolificacy and temperament were all considered important for both sheep and goats in both farming systems and across the different genotypes. Compared with other pure breeds, the Red Maasai sheep and the Small East African goats were rated poorly in terms of body size, body shape, growth and fertility, but highly in terms of drought and (for the Red Maasai) heat tolerance by both smallholder and pastoralist farmers. In general, crosses were perceived less favourably than indigenous pure breeds. Body size and performance ranked as the most important traits in the choice of breeding males. Approximately half the farmers inherited their males, reared them on the farm and kept them for an average of 2–3 years. Uncontrolled mating within the household's flock was predominant in both farming systems. Over 98% of the farmers reported incidence of disease, especially pneumonia (in pastoral/extensive areas), helminthosis, tick-borne diseases, diarrhoea and foot-rot. Over 95% of the farmers fed supplements in both dry and wet seasons. Pure exotic and indigenous × exotic genotypes fetched higher prices than indigenous genotypes due to their heavier body weight. In conclusion, the foregoing issues need attention in genetic improvement programmes, with more emphasis on the conservation and utilisation of indigenous small ruminant genotypes.     

High-density linkage mapping of vitamin E content in maize grain

Vitamin E refers to eight distinct compounds collectively known as tocochromanols and can be further divided into two classes, tocotrienols and tocopherols. Tocochromanols are the major lipid-soluble antioxidants in maize (Zea mays L.) grain. Enhancing vitamin E content of maize through plant breeding has important implications for human and animal nutrition. Four inbred lines exhibiting unique variation for tocochromanol compounds were chosen from the Goodman maize diversity panel to construct two biparental mapping populations (N6xNC296 and E2558xCo125). The N6xNC296 population was developed to analyze segregation for α-tocopherol and α-tocotrienol content. The E2558WxCo125 population was developed to analyze segregation for the ratio of total tocotrienols to tocopherols. The tocochromanol variation in two replicates of each population was quantified using liquid chromatography-diode array detection. Using high-density linkage mapping, novel quantitative trait loci (QTL) in the N6xNC296 population were mapped using tocopherol ratio traits. These QTL contain the candidate gene homogentisate phytyltransferase (ZmVTE2) within the respective support intervals. This locus was not mapped in a previous genome-wide association study that analyzed tocochromanols in the Goodman diversity panel. Transgressive segregation was observed for γ- and α-tocochromanols in these populations, which facilitated QTL identification. These QTL and transgressive segregant families can be used in selection programs for vitamin E enhancement in maize. This work illustrates the complementary nature of biparental mapping populations and genome-wide association studies to further characterize genetic variation of tocochromanol content in maize grain.     

Plant nutrient management for enhanced productivity in intensive grain production systems of the United States and Asia

Plant and Soil - Are present nutrient management recommendations for the world's major cereal cropping systems adequate to sustain the productivity gains required to meet food demand while also...     

Effects of tillage intensity on nitrogen dynamics and productivity in legume-based grain systems

In 1988 an experiment was established at the Rodale Institute Experimental Farm to study weed control and nitrogen (N) management in rotations with grain crops and N-fixing green manures under reduced tillage without the use of herbicides. Tillage intensities ranging from moldboard plow (MP) to continuous no-till (NT) were compared. We present results for maize production in 1994, the seventh year of the experiment. Our goal was to further investigate reduced tillage regimes that alternated no-till with different forms of primary tillage in legume-based systems. In the chisel-disc (CD) and MP treatments comparable yields were achieved under so-called organic (weeds controlled with cultivation and green manure N source) and conventional management (weeds controlled with herbicides and mineral N fertilizer applied). Weed competition in these treatments was minimal and the N status of maize plants was essentially the same regardless of the N source (fertilizer or green manure). Of the four organic no-till maize treatments, only the mixed-tillage system with cultivation for weed control (CD-NTc) produced yields comparable to conventional NT maize. The fate of vetch N as well as temporal N dynamics were largely determined by tillage intensity and the handling of the vetch residues at maize planting. Treatments with primary tillage (CD and MP) had extremely high levels of mineral N early in the season and had greater average net N-mineralization, even though N content of hairy vetch in these treatments was equal to or lower than that in treatments with mow-killed vetch. In terms of soil mineral N concentrations, the CD-NTc treatment was similar to the other mow-killed vetch/no-till maize treatments. However, N availability in this treatment was greater, probably due to more complete decomposition of green manure residues. Cultivation for weeds not only helped control weeds but also increased mineralization of the vetch residues, which in turn increased the N supply during the period of maximum N demand by the maize. Carefully designed rotations combining tillage reductions with the use of leguminous N sources can have multiple benefits, including improved timing of N availability, reduced herbicide applications, and improved soil quality in the long term. This revised version was published online in June 2006 with corrections to the Cover Date.     

The relationship of proline content and metabolism on the productivity of maize plants

The free proline content in maize ear-leaves, silk and pollen were analyzed in field grown plants which had matured to the pollination stage. Using maize hybrids PR34F02, PR35P12 and PR36B08 field trials were set up at two locations in eastern Croatia in two different years. Two enzymes of proline metabolism were analyzed in the same leaf samples and specific activities of synthetase (P5CS) and proline dehydrogenase (PDH). Plant productivity was evaluated at harvest by the estimation of total and fully developed grain number per ear and per plant, the mean single grain mass, and the mass of grain per plant. The year in which the plants were grown had a very significant effect on the free proline content in the leaf and pollen, as well as on the enzyme activities assayed. The differences between the plants from the two localities were very significant in all tested parameters of plant grain productivity. There was a significant genotype effect on proline content and P5CS total activity in leaf and on all the productivity parameters. Some of the correlations established suggest that the rate of proline synthesis and degradation in maize ear-leaf at pollination might contribute to the final grain production of the maize plant. Multiple regression analyses was used to further analyze the relationship between proline and grain productivity, but it is clear that future work should include other environmental conditions, plant species and organs such as roots.Key words: maize, maize silk, plant productivity, pollen, proline, proline dehydrogenase, Δ¹-pyrroline-5-carboxylate synthetase, Zea mays L.     

The effect of enhanced carotenoid content of transgenic maize grain on fungal colonization and mycotoxin content

Novel strategies that address vitamin A deficiency have been developed, such as high-carotenoid maize, a biofortified transgenic maize line rich in carotenoids generated by genetic transformation. The South African white maize inbred (M37W), which is devoid of carotenoids, was engineered to accumulate high levels of β-carotene (provitamin A), lutein, and zeaxanthin. Maize seeds contaminated with fumonisins and other mycotoxins pose a serious threat to both humans and livestock. During three consecutive harvests, the fungal incidence and the fumonisin and aflatoxin content of maize seeds grown in an experimental field in Catalonia (Northeastern Spain) were evaluated. Fungal infection was similar in high-carotenoid maize and its isogenic line, with Fusarium verticillioides being the most prevalent fungus in all the harvests. Neither Aspergillus spp. nor aflatoxin contamination was found. Fumonisin levels were lower in high carotenoid than in its isogenic line, but this reduction was statistically significant in only 2 of the 3 years of study. Our results suggest that high carotenoid content reduces fumonisin levels in maize grains.     

Effect of absence of developing grain on carbohydrate content and senescence of maize leaves

In maize (Zea mays L.) grown under normal conditions in Rhodesia, prevention of pollination or removal of the ears after flowering caused premature senescence of the leaves above the ear, preceded by the appearance of a purplish red color. In plants from which the ears had been removed the concentration of sugars and starch increased markedly in both upper and lower leaves, the increase being greater in the upper leaves.     

Soil resource heterogeneity in the form of aggregated litter alters maize productivity

Conservation agriculture in its version of permanent raised bed planting with crop residue retention increases yields and improves soil characteristics, e.g. aggregate distribution, organic matter content, so it remained to be seen how greenhouse gas emissions and dynamics of C and N might be altered. The objective of this study was to investigate how conservation agriculture with permanent raised beds, tied ridges, i.e. dykes within the furrows to prevent water run-off, and residue retention affected greenhouse gas emissions. A field experiment was started in 1999 comparing permanent and conventionally tilled raised beds with different residue management under rain fed conditions. Soil was characterized and emissions of CH₄, N₂O and CO₂ and dynamics of NH₄ ⁺, NO₂ ^? and NO₃ ^? were monitored in a laboratory experiment. The crop and tied ridges had no effect on soil characteristics and dynamics of C and N. Tilled beds reduced the water holding capacity (WHC) 1.1 times and increased conductivity 1.3 times compared to soil under nontilled beds with retention of all crop residues. The WHC, organic C, soil microbial biomass and total N were ≥1.1 larger in soil from nontilled beds where the crop residue was retained compared to where it was removed after only 6 years. The emission of CO₂ was 1.2 times and production of NO₃ ^? 1.8 times larger in nontilled beds where the crop residue was retained compared to where it was removed. The CO₂ emission was 1.2 times and the emission of N₂O after 1 day 2.3 times larger in soil under tilled beds compared to nontilled beds with full residue retention, while the increase in concentration of NO₃ ^? was 0.05 mg N kg^?1 soil in the former and 2.38 in the latter. We found that permanent raised bed planting with crop residue retention decreased emissions of N₂O and CO₂ compared to soil under conventionally tilled raised beds. Production of NO₃ ^? is larger in soil with permanent raised bed planting with crop residue retention compared to conventionally tilled raised beds.     

The role of maize root size in phosphorus uptake and productivity of maize/faba bean and maize/wheat intercropping systems

Interspecific root/rhizosphere interactions affect phosphorus (P) uptake and the productivity of maize/faba bean and maize/wheat intercropping systems. The aim of these experiments was to determine whether manipulation of maize root growth could improve the productivity of the two intercropping systems. Two near isogenic maize hybrids (the larger-rooted T149 and smaller-rooted T222) were intercropped with faba bean and wheat, under conditions of high- and low-P availability. The larger-rooted T149 showed greater competitive ability than the smaller-rooted T222 in both maize/faba bean and maize/wheat intercropping systems. The higher competitive ability of T149 improved the productivity of the maize/faba bean intercropping system in P-sufficient conditions. In maize/wheat intercropping systems, root growth, shoot biomass, and P uptake of maize were inhibited by wheat, regardless of the P-supply. Compared with T222, the larger-rooted T149 suffered less in the intercropping systems. The total biomass of the maize/wheat intercropping system was higher for wheat/T149 than for wheat/T222 under low-P conditions. These data suggested that genetic improvement of maize root size could enhance maize growth and its ability to compete for P resources in maize/faba bean and maize/wheat intercropping systems. In addition, depending on the P availability, larger maize roots could increase the productivity of intercropping systems.     

Soil management effects on entomopathogenic fungi during the transition to organic agriculture in a feed grain rotation

The growing demand for organic products creates opportunities for farmers. Information on the consequences of management practices can help farmers transition to organic and take advantage of these prospects. We examined the interaction between soil disturbance and initial cover crop on naturally occurring entomopathogenic fungi (EPF) during the 3-year transition to organic production in a feed grain rotation in central Pennsylvania. Our experiment included four systems comprised of a factorial combination of two levels of primary tillage (full vs. reduced) and two types of initial cover crop (timothy/clover vs. rye/vetch). The cropping sequence consisted of an initial cover crop, followed by soybean, and finally, maize. The entire experiment was replicated in time, with the initiation lagged by 1 year. We detected four species of EPF (Metarhizium anisopliae, Beauveria bassiana, Isaria fumosorosea, and Isaria farinosa) by bioassay of soil samples collected four times during each field season. The latter three species were detected infrequently; therefore, we focused statistical analysis on M. anisopliae. Detection of M. anisopliae varied across sampling date, year in crop sequence, and experimental start, with no consistent trend across the 3-year transition period. M. anisopliae was isolated more frequently in the systems initiated with timothy/clover cover crops and utilizing full tillage; however, we only observed a tillage effect in one temporal replicate. M. anisopliae detection was negatively associated with soil moisture, organic matter, and zinc, sulfur, and copper concentrations in the soil. This study helps to inform farmers about management effects on soil function, specifically conservation biological control.     

Crop rotation and residue management effects on carbon sequestration,nitrogen cycling and productivity of irrigated rice systems

The effects of soil aeration, N fertilizer, and crop residue management on crop performance, soil N supply, organic carbon (C) and nitrogen (N) content were evaluated in two annual double-crop systems for a 2-year period (1994–1995). In the maize-rice (M-R) rotation, maize (Zea mays, L.) was grown in aerated soil in the dry season (DS) followed by rice (Oriza sativa, L.) grown in flooded soil in the wet season (WS). In the continuous rice system (R-R), rice was grown in flooded soil in both the DS and WS. Subplot treatments within cropping-system main plots were N fertilizer rates, including a control without applied N. In the second year, sub-subplot treatments with early or late crop residue incorporation were initiated after the 1995 DS maize or rice crop. Soil N supply and plant N uptake of 1995 WS rice were sensitive to the timing of residue incorporation. Early residue corporation improved the congruence between soil N supply and crop demand although the size of this effect was influenced by the amount and quality of incorporated residue. Grain yields were 13-20% greater with early compared to late residue incorporation in R-R treatments without applied N or with moderate rates of applied N. Although substitution of maize for rice in the DS greatly reduced the amount of time soils remained submerged, the direct effects of crop rotation on plant growth and N uptake in the WS rice crops were small. However, replacement of DS rice by maize caused a reduction in soil C and N sequestration due to a 33–41% increase in the estimated amount of mineralized C and less N input from biological N fixation during the DS maize crop. As a result, there was 11–12% more C sequestration and 5–12% more N accumulation in soils continuously cropped with rice than in the M-R rotation with the greater amounts sequestered in N-fertilized treatments. These results document the capacity of continuous, irrigated rice systems to sequester C and N during relatively short time periods. This revised version was published online in June 2006 with corrections to the Cover Date.     

Dietary zinc effects on zinc, calcium, and magnesium content in bones of growing rats

The aim of the present study was to assess dietary zinc effects on femur weight and mineral content in growing rats. For this purpose, 70 weanling Sprague-Dawley rats were divided into four groups. Each group was subject to a diet containing 2 (BZ), 5 (DZ), 10 (MZ), and 30 (CZ) ppm zinc. The calcium and magnesium content in all diets was 5 g/kg and 507 mg/kg, respectively. The animals were kept on this regime for 28 d and then sacrificed and their femurs were removed for analysis using atomic absorption spectrophotometry. The weights of the BZ and DZ groups were significantly different from the MZ and CZ groups (38.5±10.5, 89.9±13.7, 118.6±13.6 and 134±19.9 g, p<0.01) respectively. There were no differences between the MZ and CZ groups. Femur weight also varied with dietary zinc, as it was significantly different among all groups (BZ, 265±49 mg; DZ, 380±40 mg; MZ, 452±54 mg; CZ, 735±66 mg; p<0.01). The femur zinc content varied with diets, following a different pattern than the above parameters. Femur zinc from the BZ group (51.5±5.4 ppm) was significantly different from the MZ and CZ groups (115.9±14.2 and 175.0±13.5 ppm, respectively), whereas the DZ group (62.5±11.3 ppm) did not differ from the other three groups. The femur content of calcium (BZ, 83.2±9.8 mg/g; DZ, 88.0±9.2 mg/g; MZ, 90.2±13.6 mg/g; CZ, 83.1±14.7 mg/g) and magnesium (BZ, 1.82±0.13 mg/g; DZ, 1.98±0.09 mg/g; MZ, 1.93±14 mg/g; CZ, 1.83±0.19 mg/g) were not significantly different among the groups, nor was the calcium-magnesium ratio. These results suggest that although dietary zinc deficiency retards growth and causes bone fragility, bone deposition of calcium and magnesium and its ratio are not affected.     

Soil acidity effects on nutrient use efficiency in exotic maize genotypes

Maize (t Zea mays L.) is the third most important cereal grown in the world. In South and Central America, maize is mostly grown on acidic soils. On these soils, yields are limited by deficient levels of available P, Ca, and Mg, and toxic levels of Al and Mn. A greenhouse study was conducted with 22 maize genotypes originating from Africa, Europe, and North, Central, and South America on acid, dark red latosol (Typic Haplorthox) at 2%, 41%, and 64% Al saturation at corresponding pH of 5.6, 4.5, and 4.3. With increasing Al levels, the nutrient efficiency ratios (NER = mgs of dry shoot weight / mg of element in shoot) for K, Ca and Mg increased, and NER for P and Zn tended to decrease. Overall, Al-tolerant genotypes produced higher shoot and root weight and had higher NER for P, Ca Mg, and Fe at 41% Al saturation. Genotypes used in this study showed genetic diversity for growth and NER of essential nutrients. It was concluded that selection of acid-soil-tolerant genotypes and further breeding of acid-soil-tolerant maize cultivars are feasible.     

Prediction of single-cross hybrid performance for grain yield and grain dry matter content in maize using AFLP markers associated with QTL

Prediction methods to identify single-cross hybrids with superior yield performance have the potential to greatly improve the efficiency of commercial maize (Zea mays L.) hybrid breeding programs. Our objectives were to (1) identify marker loci associated with quantitative trait loci for hybrid performance or specific combining ability (SCA) in maize, (2) compare hybrid performance prediction by genotypic value estimates with that based on general combining ability (GCA) estimates, and (3) investigate a newly proposed combination of the GCA model with SCA predictions from genotypic value estimates. A total of 270 hybrids was evaluated for grain yield and grain dry matter content in four Dent × Flint factorial mating experiments, their parental inbred lines were genotyped with 20 AFLP primer-enzyme combinations. Markers associated significantly with hybrid performance and SCA were identified, genotypic values and SCA effects were estimated, and four hybrid performance prediction approaches were evaluated. For grain yield, between 38 and 98 significant markers were identified for hybrid performance and between zero and five for SCA. Estimates of prediction efficiency (R ²) ranged from 0.46 to 0.86 for grain yield and from 0.59 to 0.96 for grain dry matter content. Models enhancing the GCA approach with SCA estimates resulted in the highest prediction efficiency if the SCA to GCA ratio was high. We conclude that it is advantageous for prediction of single-cross hybrids to enhance a GCA-based model with SCA effects estimated from molecular marker data, if SCA variances are of similar or larger importance as GCA variances.     

Soil water content, maize yield and its stability as affected by tillage and crop residue management in rainfed semi-arid highlands

Rainfed crop management systems need to be optimized to provide more resilient options to cope with projected climatic scenarios forecasting a decrease in mean precipitation and more frequent extreme drought periods in Mexico. Soil water content (0?C60 cm) was measured during three crop cycles in maize plots with different agronomic management practices in a long-term rainfed experiment (established in 1991) in the highlands of Mexico. Maize yields of 1997?C2009 were reported. Crop management practices varied in (1) tillage (conventional [CT] vs. zero tillage [ZT]) and (2) residue management (full or partial retention and removal). ZT with residue retention had higher soil water content than management practices involving CT and ZT with residue removal which provided a buffer for drought periods during the growing seasons. In 2009, a cycle with a prolonged drought during vegetative growth, this resulted in yield differences of up to 4.7 Mg ha^?1 between ZT with (partial) residue retention and the other practices. Averaged over 1997?C2009, these practices had a yield advantage of approximately 1.5 Mg ha^?1 over practices involving CT and ZT with residue removal. ZT with (partial) residue retention used rainfall more efficiently and resulted in a more resilient agronomic system than practices involving either CT or ZT with residue removal.     

土壤侵蚀对土壤肥力及土地生物生产力的影响

通过对红壤坡地不同土地利用方式土壤肥力及土地生物生产力的空间分异研究,揭示了土壤侵蚀对土壤肥力和土地生产力的负面影响．即侵蚀导致Ｎ、Ｐ、Ｋ等土壤速效养分含量减少及其在坡面上部的相对贫乏和下部的相对富集;土壤有机质含量降低;土壤机械组成中砂、粉、粘粒比率发生变化,表现为土壤沙化,土地生物生产力下降．     

The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat

High grain protein content (GPC) is a frequent target of wheat breeding programmes because of its positive effect on bread and pasta quality. A wild wheat allele at the Gpc-B1 locus with a significant impact on this trait was identified previously. The precise mapping of several senescence-related traits in a set of tetraploid recombinant substitution lines (RSLs) segregating for Gpc-B1 is reported here. Flag leaf chlorophyll degradation, change in peduncle colour, and spike water content were completely linked to the Gpc-B1 locus and to the differences in GPC within a 0.3 cM interval corresponding to a physical distance of only 250 kb. The effect of Gpc-B1 was also examined in different environments and genetic backgrounds using a set of tetraploid and hexaploid pairs of isogenic lines. The results were consistent with those observed in the RSLs. The high GPC allele conferred a shorter duration of grain fill due to earlier flag leaf senescence and increased GPC in all four genetic backgrounds. The effect on grain size was more variable, depending on the genotype-environment combinations. These results are consistent with a model in which the wild-type allele of Gpc-B1 accelerates senescence in flag leaves producing pleiotropic effects on nitrogen remobilization, total GPC, and grain size.     

Soil structure formation and management effects on gas emission

The aim of this paper is to clarify the effect of soil management and thus also of soil aggregation on physical and chemical properties of structured soils both on a bulk soil scale, for single aggregates, as well as for homogenized material. Aggregate formation and aggregate strength depend on swelling and shrinkage processes and on biological activity and kinds of organic exudates as well as on the intensity, number and time of swelling and drying events. Thus, soil management like conventional or conservation tillage alter not only the mechanical strength but also the pore continuity and the hydraulic, gas and heat fluxes, and also alter the accessibility of exchange places for nutrients and for carbon storage (global change aspects). The possibility to predict physical properties on these various scales depends on the rigidity of the pore system. In general this rigidity depends on the above-mentioned physical and chemical processes both with respect to intensity and frequency, which again are linked to the soil management systems.     

Amylose content and chemical modification effects on the extrusion of thermoplastic starch from maize

The effects of starch structural properties and starch modification on extruder operation were monitored via die pressure, motor torque, mean residence time and specific mechanical energy (SME). The structural properties studied involved variations in the ratios of amylose and amylopectin as well as the effect of a hydroxypropylated starch on the fore mentioned extruder properties. A full factorial design of experiments (DOE) was used to then determine the influence of starch type (unmodified starches with 0%, 28%, 50% and 80% amylose; 80% amylose hydroxypropylated starch) and screw speed (250, 300 and 350 rpm) on these processing parameters. The effects of starch type and screw speed on extrusion operation that were systematically investigated using the DOE and have provided valuable insight into the relationships between starch structure and processing. The design of experiments showed that starch type for both unmodified and modified maize had a statistically significant effect on parameters such as torque, die pressure and specific mechanical energy and that screw speed also significantly effected specific mechanical energy. Residence time distributions differed according to starch type (amylose content, hydroxypropylation) and screw speed. The additional study of residence time distribution also gave an indication of the degree of mixing in the extruder. Starch type variations were apparent at low screw speed however at higher screw speed the influence of starch type decreased significantly.