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江浙沪和哈尔滨地区汉族D17S30位点的多态性分布 总被引:14,自引:1,他引:13
D17S30是位于人类染色体17d13.3、以70bp为重复单位的,具有高度多态性的VNTR位点,本文采用作者报道的微量快速Amp-FLP分型技术,对100名哈尔滨市北方汉族人和110名江浙沪南方汉族人作了D17S30位点分型,发现在北方汉族与江浙沪南方汉族之间等位基因频率分布并无显著差异,但可见A1和A7基因频率北高南低,A4基因频率则为南高北代,提示存在南北汉族之间的分化。D17S30位点南北 相似文献
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利用RFLP、SSR、AFLP和RAPD标记分析玉米自交系遗传多样性的比较研究 总被引:115,自引:3,他引:112
利用 RFLP、SSR.AFLP和RAPD 4种分子标记方法研究了 15个玉米(Zea mays L.)自交系的遗传多样性,同时对4种标记系统进行比较。在供试材料中筛选到具多态性的RFLP探针酶组合56个,66对SSR引物,20个RAPD引物和9个AFLP引物组合,分别检测到多态性带167、201、87和108条。SSR标记位点的平均多态性信息量(PIC)最大(0.54),AFLP标记位点最小(0.36),但AFLP标记具有最高的多态性检测效率(Ai,32.2)。4种分子标记所得遗传相似系数相关性显著,比较相关系数表明 RAPD可靠性较低。依据 4种分子标记结果将 15个供试自交系划分为塘四平头、旅大红骨、兰卡斯特、瑞德和PN共5个类群,与系谱分析基本一致。认为SSR和RFLP两种分子标记方法适合进行玉米种质遗传多样性的研究。 相似文献
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MTHFR基因多态性与动脉粥样硬化性脑梗塞的关系 总被引:8,自引:0,他引:8
采用PCR- RFLP技术,检测了62 例动脉粥样硬化性脑梗塞患者和79 名对照者的C677T 突变的基因型。结果发现, MTHFR基因C677T 突变型等位基因(V)频率在实验组和对照组中,有显著性差异(χ2= 4.41,P< 0.05);三种基因型频率在两组人群中均无显著性差异。基因型频率的相对风险分析,AV基因型比AA 基因型患脑梗塞风险高1.76 倍;VV 基因型比AA 基因型患脑梗塞风险高3.25 倍。结果表明, MTHFR 基因C677T 突变型等位基因与动脉粥样硬化性脑梗塞有一定的关联,突变基因型增加了动脉粥样硬化脑梗塞的发病风险。 相似文献
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利用AFLP遗传连锁图定位大麦苗期对叶锈病的部分抗性基因 总被引:11,自引:0,他引:11
借助大麦染色体AFLP标记遗传连锁图和MapQTLV3.0作图软件,对大麦叶病的数量抗性基因进行了定位分析,明确了大麦部分抗性品种Vada对叶锈病的潜育期由分别位于染色体1、2、6、7上离短臂末端79cM、186cM、58cM和117cM处的4个数量抗性基因所控制。 相似文献
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SlNPV p10基因簇的序列及结构特征分析(英文) 总被引:2,自引:1,他引:1
以病毒感染后期的cDNA为探针,将SlNPVp10基因杂交定位于基因组DNA的BamHI-3.0kbp片段上。SlNPVp10基因长318nt,编码一105aa的P10蛋白,为目前发现的最长的p10基因;基因的启动子内含有两个ATTGTA模体(motif),是目前发现的p10基因中唯一含有两个A/TTTGTA模体者。SlNPVP10蛋白含有10个七肽重复单元,可形成一个相对较长的卷曲螺旋。SlNPVp10所在的ORF552-p10-ORF945基因簇与SpliNPV的ORF552-p10-ORF945基因簇各基因座位、方向及对应的氨基酸序列十分相似,表明SlNPV与SpliNPV有很近的亲缘关系。 相似文献
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用PCR—RFLP方法研究藏族HLA0—DQA1和—DQB1基因多态性 总被引:4,自引:1,他引:3
应用目前HLA研究领域中成熟的、有效的PCR-RFLP基因分型技术,从DNA水平对藏族健康群体进行了HLA-DQA1(49人)和-DQB1(49人)基因分型,这在国内外属首次。所采用的PCR-RFLP基因分型技术是在HLA-DQA1和-DQB1各等位基因全部序列已知的情况下,对其第2个外显子碱基序列扩增进而进行RFLP分析的方法。这种方法得到的RFLP的所有片段都是已知序列,因而精确度很高,同时为 相似文献
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以栽培稻的8个籼-粳测验种为对照,采用39对SSR引物检测了江永野生稻居群在1982年、2008年、2017年的遗传多样性,采用38对In Del引物检测了江永野生稻居群在1982年、2008年、2017年的籼-粳基因频率。结果表明:在1982年取样保存在异位圃的40份样本的遗传多样性稍高于2008年、2017年原位保护区样本的遗传多样性;2008年取的样本数虽然比2017年多,但两次取的样本之间遗传多样性几乎没差异。不同年份取的样本之间的遗传分化系数Fst都很小,基因流Nm都较大,分化不明显。通过聚类分析和主坐标分析(PCo A),发现野生稻居群与4份栽培粳稻聚为一类,4份栽培籼稻单独聚成一类,显示江永野生稻与粳稻的血缘近于籼稻;籼-粳基因频率的分析表明,野生稻样本多属粳稻型,少数属偏粳稻型,原位保护区的偏粳稻类型单株数占取样单株总数的比例,2008年比1982年的增加了10.0%,2017年比2008年的增加了1.6%,显示江永野生稻原位保护区生境条件有利野生稻从粳稻型向偏粳稻型变异,随着野生稻产生环境适应性变异,籼型基因频率在提高。 相似文献
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鲑鱼胰岛素样生长因子I cDNA在镜鲤中的表达 总被引:2,自引:0,他引:2
通过显微注射技术,将冠以鲤鱼β-肌动蛋白基因(CA)启动调控顺序的鲑鱼胰岛素样生长因子I(sIGF-I)的cDNA,即CAsIGFc注入镜鲤受精卵内,经聚合酶链式反应(PCR)技术检测,受本胚胎发育各期均携有外源基因拷贝。 相似文献
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B. S. Weir 《Genetics》1992,130(4):873-887
An analysis is presented of data collected by the Federal Bureau of Investigation at six unlinked variable number of tandem repeats (VNTR) loci for the United States population. Databases have been constructed of VNTR profiles of Caucasians, Blacks and Hispanics from Florida, Texas and California. There was very little evidence for correlations between lengths for pairs of VNTR fragments, within or between loci. When the fragment lengths were amalgamated into discrete bins, there was also little evidence for disequilibrium over all genotypes, within or between loci, for the Caucasian database, although some disequilibrium was found for the Black and Hispanic databases. No disequilibrium was found for the Caucasian or Black databases when tests were confined to heterozygous individuals. In cases of global disequilibrium, local tests can be applied to specific genotypes. The results suggest that, at the bin level, frequencies of VNTR profiles can generally be estimated as the products of the frequencies of the constituent elements. This overcomes the problem of estimating population frequencies when any particular profile does not exist in the database. There is some evidence for different frequencies, at the individual bin level, between geographic samples within each of the Caucasian, Black and Hispanic databases, and considerable evidence for differences between the three databases. These differences are less evident for the frequencies of four-locus profiles. 相似文献
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人核糖体DNA打靶载体pHr是一种由中南大学医学遗传学国家重点实验室开发构建的针对人类基因组的同源重组质粒载体.利用pHr构建了一种mda-7/GFP融合基因的人源基因表达载体pHr-CMG,并研究了其在肝癌细胞系Bel-7402中的作用.利用荧光显微镜、RT-PCR和Western blotting检测了mda-7/GFP融合基因的表达;利用细胞周期分析、MTT和Hoechst33258染色研究了其在细胞中的作用.结果显示,pHr-CMG载体能在Bel-7402细胞中有效表达MDA-7/GFP融合蛋白,进而抑制细胞增殖和诱导细胞凋亡,推测其可能是由载体表达了mda-7基因引起细胞在G2/M期累积所导致的.同时,实验结果证实了人核糖体DNA打靶载体系统以及pHr-CMG表达载体的有效性,为其在进一步基因治疗研究中的应用提供了理论和实验基础. 相似文献
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中国人COL2A1基因座的扩增片段长度多态性 总被引:1,自引:0,他引:1
用聚合酶链式反应、高分辨聚丙烯酰胺凝胶水平电泳及银染技术对位于人类Ⅱ型胶原基因终止密码下游非转录区1.3kb处的可主数目串联重复育列进行了研究。制备了由人类不同基因型DNA混合而成的人类等位基因型参考物,根据实验结果进行了命名。 相似文献
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Resistance gene analogues from rice: cloning, sequencing and mapping 总被引:18,自引:0,他引:18
R. Mago S. Nair M. Mohan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):50-57
Degenerate oligonucleotide primers were designed on the basis of nucleotide-binding-site (NBS) motifs conserved between resistance
genes of Arabidopsis, flax and tobacco and subsequently used as PCR primers to amplify resistance gene analogues (RGA) in rice. Primers amplified
a major band of approximately 500 bp. Restriction analysis of the amplified product revealed that the band was made up of
several different fragments. Many of these fragments were cloned. Sixty different cloned fragments were analysed and assigned
to 14 categories based on Southern blot analysis. Fourteen clones, each representing one of the 14 categories of RGAs were
mapped onto the rice genetic map using a Nipponbare ( japonica)בKasalath’ (indica) mapping population consisting of 186 F2 lines. Of the 14 clones representing each class 12 could be mapped onto five different chromosomes of rice with a major cluster
of 8 RGAs on chromosome 11. Our results indicate that it is possible to use sequence homology from conserved motifs of known
resistance genes to amplify candidate resistance genes from diverse plant taxa.
Received: 23 September 1998 / Accepted: 28 November 1998 相似文献
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中国人eNOS基因VNTR多态性的基因型与等位基因频率 总被引:1,自引:0,他引:1
一氧化氮合酶(nitric oxide synthase,NOS)催化L-精氨酸的氧化反应生成L-瓜氨酸和一氧化氮(nitric oxide,NO)。NO可通过cGMP依赖的信号传导途径介导平滑肌细胞舒张,是调节血管张力的重要信使分子。NO尚可抑制血小板凝集,对血栓形成起重要调节作用。目前在哺乳动物中已发现细胞来源、表达方式和活性调节不同的3种NOS同工酶,分别为神经元型NOS(neuronal NOS,nNOS)、诱导型NOS(inducible NOS,iNOS)和内皮细胞型NOS(endothelial NOS,eNOS)。人的eNOS基因位于第7号染色体长臂(7q36),全长约21kb,含有26个外显子和25个内含子。eNOS基因存在多个与心脑血管疾病相关的基因多态性位点。其中位于第4内含子的一个以27bp为核心的数目可变性串联重复序列(variable number of tandem repeat,VNTR)多态性位点,已被证实与原发性高血压、心肌梗死和静脉血栓形成有关。目前在我国尚缺乏NOS基因多态性在正常人群中基因型及等位基因频率分布的统计资料。为此,我们从316名健康中国人的基因组DNA检测了eNOS基因第4内含子VNTR多态性的基因型和等位基因,鉴定出重复6次、5次和4次的3种等位基因,以及6/5杂合、5/5纯合、5/4杂合和4/4纯合的4种基因型。同时我们将正常中国人eNOS基因VNTR多态性的基因型和等位基因频率与其他种族的相关资料进行了统计对比。结果表明,中国人eNOS基因VNTR的各种基因型和等位基因频率与日本人相似,4/4纯合基因型频率与高加索人差异显著,各种基因型和等位基因频率与非裔美国人均存在显著差异。 相似文献
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WANG YiWen LIU Yan GAO YanXia CHEN Jing ZHANG Wenxin & LIN ChongDe 《中国科学:生命科学英文版》2008,51(1):72-79
Understanding others mind and interpersonal interaction are the cognitive basis of successful social interactions. People's mental states and behaviors rely on their holding beliefs for self and others. To investigate the neural substrates of false belief reasoning, the 32 channels event-related potentials (ERP) of 14 normal adults were measured while they understood false-belief and true belief used de-ceptive appearance task. After onset of the false-belief or true-belief questions, N100, P200 and late negative component (LNC) were elicited at centro-frontal sites. Compared with true belief, false belief reasoning elicited significant declined LNC in the time window from 400 to 800 ms. The source analysis of difference wave (False minus True) showed a dipole located in the middle cingulated cortex. These findings show that false belief reasoning probably included inhibitive process. 相似文献
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Genotypic analysis of families with lactate dehydrogenase A(M) deficiency by selective DNA amplification 总被引:2,自引:2,他引:0
Summary Genomic DNA prepared from LDH-A-deficient whole blood was amplified by the polymerase chain reaction technique using two primers specific for the active human LDH-A gene. The amplified fragment was examined by direct agarose gel electrophoresis, and a deletion of 20 base pairs (bp) in exon 6 of the LDH-A gene was found. The results permitted a clear distinction between the homozygous mutant, the heterozygous mutant, and wild-type genotypes. Moreover, HinfI digestion and direct sequencing of the amplified product confirmed the results from direct agarose gel electrophoresis. Four families, including 18 individuals, were shown to contain the same mutation, that is a 20-bp deletion in exon 6. All genotypes were consistent with their biochemical phenotypes as evaluated by the ratio of LDH-B to LDH-A subunits in erythrocytes. Thus, all four known affected families in Japan have been shown to carry the same mutant gene, which may have been derived from a single mutational event. 相似文献
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Zu YuanGang Gao ChongYang Wang WenJie Yang FengJian Liu Ying Wang Min Zhao YangGuo 《中国科学:生命科学英文版》2007,50(1):22-30
The traditional culture-dependent plate counting and culture-independent small-subunit-ribosomal RNA gene-targeted molecular
techniques, Single-Strand Conformation Polymorphism (SSCP) and terminal Restriction Fragment Length Polymorphism (tRFLP) combined
with 16S rDNA clone library were adopted to investigate the impacts of secretion from Camptotheca acuminata (abbreviated to Ca) roots on the quantities and structure of eukaryotic microbes and bacteria in the rhizosphere, and the
possibility that Ca controls exotic invasive plant Eupatorium adenophorum (Ea). The counting results indicated that the number of bacteria increased in turn in rhizospheres of Ea, Ca-Ea mixed culture
and Ca, while that of eukaryotic microbes decreased. PCR-SSCP profiles showed eukaryotic microbial bands (corresponding to
biodiversity) in rhizosphere of Ea were more complex than those of Ca and CE. Meristolohmannia sp., Termitomyces sp. and Rhodophyllus sp. were the dominant populations in the rhizosphere of Ca. Bacterial terminal restriction fragments (TRFs) profiles showed
no difference among three kinds of rhizospheres, and the sequences of the 16S rDNA clone library from Ca rhizospheres were
distributed in 10 known phyla, in which phylum Proteobacteria were the absolute dominant group and accounted for 24.71% of
the cloned sequences (δ-Proteobacteria accounted for up to 17.65%), and phyla Acidobacteria and Bacteroidetes accounted for
16.47% and 10.59% of the cloned sequences, respectively. In addition, high performance liquid chromatography detected a trace
amount of camptothecin and hydroxycamptothecin in the rhizospheric soil of Ca and CE, but examined neither camptothecin nor
hydroxycamptothecin in rhizospheric soil of Ea. Therefore, invasion and diffusion of Ea evidently depended on distinguishing
the eukaryotic community structure, but not on that of the bacterial pattern. Ca was able to alter the eukaryotic community
structure of invasive Ea by secreting camptothecin and hydroxycamptothecin into rhizospheres, and may benefit the control
of overspread of Ea. This study provided theoretical evidence for rhizospheric microbial aspects on substituting Ca for Ea.
Supported by the Excellent Young Teacher’s Innovation Foundation of Northeast Forestry University to Yang FengJian, the Key
Research Fund of Ministry of Education of China (Grant No. 104191) and the Forestry Noxious Plant Investigation Fund of State
Forestry Administration of China to Zu YuanGang 相似文献