共查询到20条相似文献,搜索用时 8 毫秒
1.
Dr. Robert D. Burke 《Cell and tissue research》1978,191(2):233-247
Summary Tissues that have the ultrastructural characteristics of nervous tissues are associated with ciliary and muscular elements of the pluteus larva of Strongylocentrotus purpuratus. The nerve cells are found along the margins of the ciliary bands, which are composed predominantly of spindle-shaped ciliated cells. The nerve cells contribute axonal processes to a tract of axons, which runs at the base of the ciliary band throughout its length. Axonal tracts, in the esophagus, lie beneath the circumesophageal muscles. Branched microvilli, which have been interpreted as sensory receptors, are located on the oral side of the main ciliary band and connect with the nerve cells in the ciliary band. The nervous structures described here, and other tissues of the pluteus that have been previously described as nervous, are compared on the basis of their association with receptor and effector organs, and their ultrastructural characteristics. 相似文献
2.
ROBERT D. BURKE 《Invertebrate reproduction & development.》2013,57(1):13-21
The 3 elements of the digestive tract of the pluteus larva (esophagus, stomach and intestine) acquire their characteristic shapes between 60 and 72 h after fertilization (15°C). The number of cells in the developing gut increases sigmoidally from about 100 at the completion of gastrulation to about 425 in the feeding pluteus. Embryos in which cytokinesis was inhibited with colchicine were able to form typically shaped guts comprising about 150 cells. Autoradiographs of embryos that had been cultured in sea water containing [3H]thymidine during shaping of the gut indicated no regions of enhanced thymidine incorporation. The hypothesis that shaping of the larval gut is due to regions of allometric cell proliferation was rejected. Measurements of the length of the developing gut indicate that the bending of the gut is facilitated by a doubling of the length of the digestive tract between two fixed points, the mouth and the anus. 相似文献
3.
Summary The distribution of FMRFamide-like immunoreactive neurons in the nervous system of the slug Limax maximus was studied using immunohistochemical methods. Approximately one thousand FMRFamide-like immunoreactive cell bodies were found in the central nervous system. Ranging between 15 m and 200 m in diameter, they were found in all 11 ganglia of the central nervous system. FMRFamide-like immunoreactive cell bodies were also found at peripheral locations on buccal nerve roots. FMRFamide-like immunoreactive nerve fibres were present in peripheral nerve roots and were distributed extensively throughout the neuropil and cell body regions of the central ganglia. They were also present in the connective tissue of the perineurium, forming an extensive network of varicose fibres. The large number, extensive distribution and great range in size of FMRFamide-like immunoreactive cell bodies and the wide distribution of immunoreactive fibres suggest that FMRFamide-like peptides might serve several different functions in the nervous system of the slug. 相似文献
4.
Summary Development of the esophageal muscles in embryonic sea urchins is described using light- and electron microscopy. The muscles develop from processes of about 14 cells of the coelomic epithelium that become immunore-active to anti-actin at about 60 h (12–14° C). Initially, eachmyoblast extends a single process with numerous fine filopodia around the esophagus. By 72 h the processes have reached the midline and fused with those from cells of the contralateral coelomic sac. Myoblasts begin to migrate out of the coelomic epithelium between 72 and 84 h. By 72 h the processes stain with the F-actin specific probe NBD-phallacidin. The contractile apparatus is not evident in transmission electron-microscopic preparations of embryos at 70 h, but by 84 h the contractile apparatus is present and the muscle cells are capable of contraction. Because the myoblasts migrate free of the coelomic epithelium and are situated on the blastocoelar side of the basal lamina, it is suggested that that they should be considered as a class of mesenchymal cells. 相似文献
5.
Antibacterial activity in Strongylocentrotus droebachiensis (Echinoidea), Cucumaria frondosa (Holothuroidea), and Asterias rubens (Asteroidea) 总被引:3,自引:0,他引:3
Haug T Kjuul AK Styrvold OB Sandsdalen E Olsen ØM Stensvåg K 《Journal of invertebrate pathology》2002,81(2):94-102
A search for antibacterial activity in different body parts of the green sea urchin Strongylocentrotus droebachiensis, the common starfish Asterias rubens, and the sea cucumber Cucumaria frondosa was conducted. Antibacterial activity was detected in extracts from several tissues in all species tested, but mainly in the coelomocyte and body wall extracts. Relatively high antibacterial activity could also be detected in gastrointestinal organs and eggs from A. rubens and in eggs from C. frondosa. Differences between active extracts regarding hydrophobicity and sensitivity to heat and proteinase K treatment indicated that several different compounds were responsible for the antibacterial activities detected. Lysozyme-like activity could be detected in several tissues from A. rubens. Haemolytic activity could be detected in all species tested, especially in the body wall extracts. Results from the current study suggest that marine echinoderms are a potential source for the discovery of novel antibiotics. 相似文献
6.
I. I. Deak 《Development genes and evolution》1977,183(2):165-169
Summary Mitotic recombination was induced, by X-irradiation at the blastoderm stage, in flies heterozygous for one of the temperature-sensitive paralytic mutationsshibire andtp-2. The results show that these mutations can be used to detect the presence of clones in the central nervous system through the temperature-sensitive paralysis of individual legs. Mitotic recombination can also be used to examine the effects of these mutations in the peripheral nervous system; shibire is thus shown to affect the function of sensory neurons. 相似文献
7.
Neuregulins(NRG)是一在结构上相类似的多肽家族,它们由四个不同的基因编码。NRG有三种异构体(NRG1、2和3)。这些异构体在及脑内有高表达,包括发育中的脑和成熟的脑。这些异构体中,对NRG1研究最为广泛。NRG1对神经系统的发育有多种重要的调节作用。它能促进胶质细胞的生化和分化;也能调节小脑、颗粒细胞沿胶质细胞的迁移。在突触形成过程中,NRG1可诱导以下三种受体的表达:神经肌肉接头和中枢 神经系统内的乙酰胆碱受体的表达;小脑胶质细胞中NMDA受体的NR2C亚单位的表达;小脑胶质细胞中GABAA受体的表达。近年来的研究表明,NRG受体多分布于突触后膜致密区,提示NRG可能对突触的可塑性有重要的作用。本文综述了NRG的研究进展,特别对其功能及其信号转导机制有较多的讨论。 相似文献
8.
We documented expression of the pan-metazoan neurogenic gene engrailed in larval and juvenile Patiriella sea stars to determine if this gene patterns bilateral and radial echinoderm nervous systems. Engrailed homologues, containing conserved En protein domains, were cloned from the radial nerve cord. During development, engrailed was expressed in ectodermal (nervous system) and mesodermal (coeloms) derivatives. In larvae, engrailed was expressed in cells lining the larval and future adult coeloms. Engrailed was not expressed in the larval nervous system. As adult-specific developmental programs were switched on during metamorphosis,
engrailed was expressed in the central nervous system and peripheral nervous system (PNS), paralleling the pattern of neuropeptide
immunolocalisation. Engrailed was first seen in the developing nerve ring and appeared to be up-regulated as the nervous system developed. Expression of
engrailed in the nerve plexus of the tube feet, the lobes of the hydrocoel along the adult arm axis, is similar to the reiterated pattern
of expression seen in other animals. Engrailed expression in developing nervous tissue reflects its conserved role in neurogenesis, but its broad expression in the adult
nervous system of Patiriella differs from the localised expression seen in other bilaterians. The role of engrailed in patterning repeated PNS structures indicates that it may be important in patterning the fivefold organisation of the ambulacrae,
a defining feature of the Echinodermata. 相似文献
9.
P. N. M. Konings Dr. H. G. B. Vullings M. Geffard R. M. Buijs J. H. B. Diederen W. F. Jansen 《Cell and tissue research》1988,251(2):371-379
Summary The distribution of octopamine in the metathoracic ganglion, brain and corpus cardiacum of Locusta migratoria and Schistocerca gregaria was investigated by means of immunocytochemistry with an antiserum against octopamine. The dorsal unpaired median (DUM) cells of the metathoracic ganglion were found to be strongly octopamine-immunoreactive. In the rostroventral part of the protocerebrum a group of seven immunopositive cells was demonstrated. Stained nerve fibres of these cells run into three directions: circumoesophageal connectives, midbrain, and optic lobes. As far as the protocerebrum is concerned, immunoreactive fibres were found in the central body, the protocerebral bridge, and in other neuropile areas. In the optic lobe a dense plexus of immunopositive fibres was found in the lobula and in the medulla. In the brain one other immunopositive cell was demonstrated, situated at the lateral border of the tritocerebrum. Octopamine could not be shown to occur either in the globuli cells of the mushroom bodies or in the dorsolateral part of the protocerebrum, where the perikarya of the secretomotor neurones are located that innervate the glandular cells of the corpus cardiacum. In the nervi corporis cardiaci II, which contain the axons of the neurones that extend into the glandular part of the corpus cardiacum, and in the corpus cardiacum proper no specific octopamine immunoreactivity could be found. 相似文献
10.
Streit A 《Developmental biology》2004,276(1):1-15
Sensory placodes are unique columnar epithelia with neurogenic potential that develop in the vertebrate head ectoderm next to the neural tube. They contribute to the paired sensory organs and the cranial sensory ganglia generating a wide variety of cell types ranging from lens fibres to sensory receptor cells and neurons. Although progress has been made in recent years to identify the molecular players that mediate placode specification, induction and patterning, the processes that initiate placode development are not well understood. One hypothesis suggests that all placode precursors arise from a common territory, the pre-placodal region, which is then subdivided to generate placodes of specific character. This model implies that their induction begins through molecular and cellular mechanisms common to all placodes. Embryological and molecular evidence suggests that placode induction is a multi-step process and that the molecular networks establishing the pre-placodal domain as well as the acquisition of placodal identity are surprisingly similar to those used in Drosophila to specify sensory structures. 相似文献
11.
In the developing pluteus larva of S. purpuratus, the initial morphogenetic event in the formation of a functional gut is the appearance of two constrictions in the archenteron. These two constrictions become the cardiac and pyloric sphincters. During the 2 h in which the constrictions form, the sphincter cells change from cuboidal to wedge-shaped, and the apical ends of the sphincter cells develop an electron-dense region in which microfilaments can be resolved. Constriction of the archenteron was reversibly inhibited by cytochalasin B, although cytochalasin B had no effect once the constrictions had fully formed. Neither the electron-dense region nor the microfilaments were observed after cytochalasin B treatment. It is suggested that sphincter formation is initially accomplished by a microfilament-mediated contraction of the apical ends of the sphincter cells, which changes their shape and constricts the archenteron. 相似文献
12.
13.
L. Hernádi 《Cell and tissue research》1994,277(1):189-198
Gamma-aminobutyric acid (GABA)-like immunoreactive neurons were studied in the central and peripheral nervous system of Helix pomatia by applying immunocytochemistry on whole-mount preparations and serial paraffin sections. GABA-immunoreactive cell bodies were found in the buccal, cerebral and pedal ganglia, but only GABA-immunoreactive fibers were found in the viscero-parietal-pleural ganglion complex. The majority of GABA-immunoreactive cell bodies were located in the pedal ganglia but a few could be found in the buccal ganglia. Varicose GABA-ir fibers could be seen in the neuropil areas and in distinct areas of the cell body layer of the ganglia. The majority of GABA-ir axonal processes run into the connectives and commissures of the ganglia, indicating an important central integrative role of GABA-immunoreactive neurons. GABA may also have a peripheral role, since GABA-immunoreactive fibers could be demonstrated in peripheral nerves and the lips. Glutamate injection did not change the number or distribution of GABA-immunoreactive neurons, but induced GABA immunoreactivity in elements of the connective tissue ensheathing the muscle cells and fibers of the buccal musculature. This shows that GABA may be present in different non-neural tissues as a product of general metabolic pathways. 相似文献
14.
SchistoFLRFamide (PDVDHVFLRF-NH2) is one of the major endogenous neuropeptides of the FMRF-amide family found in the nervous system of the locust,Schistocerca gregaria. To gain insights into the potential physiological roles of this neuropeptide we have examined the distribution of SchistoFLRFamide-like immunoreactivity in the ventral nervous system of adult locusts by use of a newly developed N-terminally specific antibody. SchistoFLRFamide-like immunoreactivity in the ventral nerve cord is found in a subgroup of the neurones that are immunoreactive to an antiserum raised against bovine pancreatic polypeptide (BPP). In the suboesophageal ganglion three groups of cells stain, including one pair of large posterior ventral cells. These cells are the same size, in the same location in the ganglion and have the same branching pattern as a pair of BPP immunoreactive cells known to innervate the heart and retrocerebral glandular complex of the locust. In the thoracic and abdominal ganglia two and three sets of cells, respectively, stain with both the SchistoFLRFamide and BPP antisera. In the abdominal ganglia the immunoreactive cells project via the median nerves to the intensely immunoreactive neurohaemal organs. 相似文献
15.
The distribution of GABA-like immunoreactivity in the thoracic nervous system of the locust Schistocerca gregaria 总被引:1,自引:0,他引:1
Dr. A. H. D. Watson 《Cell and tissue research》1986,246(2):331-341
Summary An antiserum raised against gamma aminobuyric acid (GABA) was used to stain the thoracic nervous system of the locust. It stained both neuronal somata and processes within the neuropile. Among the stained somata, those of the three pairs of common inhibitory motor neurones could be identified in each of the three thoracic ganglia. In the pro- and mesothoracic ganglia five discrete groups of somata are stained, four ventral and one dorsal. In the metathoracic neuromere, an additional second dorsal group can be identified. In the abdominal neuromeres of the metathoracic ganglion both dorsal and ventral somata are stained but the latter cannot be divided into discrete populations. In each ganglion, dorsal commissures (DC) IV and V are composed of stained neurites, DCVII, the supramedian commissure, the perpendicular tract, and all the longitudinal tracts contain both stained and unstained neurites. DCI, II, III and VI, the T and I tracts are unstained. An abundance of GABA-like immunoreactive processes is found throughout the neuropile except for the anterior ventral association centre where stained processes are sparser. Some of the stained cell groups contain neurones that have been studied physiologically. The function of these neurones is discussed.Beit Memorial Fellow 相似文献
16.
Marie-Claude Senut Seema Azher Frank L. Margolis Kamakshi Patel Ahmad Mousa Arshad Majid 《Cell and tissue research》2009,337(1):45-61
Carnosine-like peptides (carnosine-LP) are a family of histidine derivatives that are present in the nervous system of various
species and that exhibit antioxidant, anti-matrix-metalloproteinase, anti-excitotoxic, and free-radical scavenging properties.
They are also neuroprotective in animal models of cerebral ischemia. Although the function of carnosine-LP is largely unknown,
the hypothesis has been advanced that they play a role in the developing nervous system. Since the zebrafish is an excellent
vertebrate model for studying development and disease, we have examined the distribution pattern of carnosine-LP in the adult
and developing zebrafish. In the adult, immunoreactivity for carnosine-LP is specifically concentrated in sensory neurons
and non-sensory cells of the olfactory epithelium, the olfactory nerve, and the olfactory bulb. Robust staining has also been
observed in the retinal outer nuclear layer and the corneal epithelium. Developmental studies have revealed immunostaining
for carnosine-LP as early as 18 h, 24 h, and 7 days post-fertilization in, respectively, the olfactory, corneal, and retinal
primordia. These data suggest that carnosine-LP are involved in olfactory and visual function. We have also investigated the
effects of chronic (7 days) exposure to carnosine on embryonic development and show that 0.01 μM to 10 mM concentrations of
carnosine do not elicit significant deleterious effects. Conversely, treatment with 100 mM carnosine results in developmental
delay and compromised larval survival. These results indicate that, at lower concentrations, exogenously administered carnosine
can be used to explore the role of carnosine in development and developmental disorders of the nervous system.
This research was supported in part by an Intramural Research Grant Program Award from Michigan State University (no. 06-IRGP-899
to M.C.S.) and a grant from the National Institutes of Health (no. DC-03112 to F.L.M.). 相似文献
17.
18.
In this study, immunohistochemistry on Vibracut sections is used to demonstrate anti-histamine immunoreactivity in the brain of the spider, Cupiennius salei (Keys.) (Ctenidae). We describe a system of histamine-immunoreactive neurons within the central nervous system that consists of six omnisegmental neurons. These histamine-immunoreactive neurons form two subgroups: a dorsal system with two cells per hemisphere and a ventral system with only one cell per hemisphere. The cells have extended arborizations in the motor and sensory areas of all neuromeres in the suboesophageal ganglionic mass. We have also found histamine immunoreactivity in the photoreceptors of C. salei and suggest that histamine is a neurotransmitter of photoreceptors in all arthropods, since it is also known to occur in the photoreceptors of the other main arthropod taxa (Merostomata, Crustacea, and Insecta). 相似文献
19.
Summary Immunohistochemical techniques were used to study the distribution of gamma-amino butyric acid (GABA)-like immunoreactive neurons in the nervous system of the slug Limax maximus. Approximately 170 GABA-like immunoreactive cell bodies were found in the central nervous system. These were located in the cerebral, buccal and pedal ganglia. Most GABA-like immunoreactive neurons had small cell bodies, which were aggregated into discrete clusters within the cerebral and pedal ganglia. Three pairs of longer, uniquely identifiable, GABA-like immunoreactive cells were found in the cerebral ganglion. GABA-like immunoreactive nerve fibres were also found in all of the central ganglia but were absent from peripheral nerves. These results suggest that GABA acts as a central neurotransmitter in the slug. The possible roles of GABA-ergic neurotransmission in the slug are discussed. 相似文献
20.
The morphological patterns of the cultivated cells of primary mesenchyme and the spicules of the larval skeleton of the sea urchin Strongylocentrotus nudus were quantified, and the value of their fractal dimensions (D) was determined with ImageJ 1.20s software. It was shown that during cytodifferentiation, the values of D in the fractal (fractional) dimension, which reflects the complex spatial organization of the spiculogenous mesenchyme elements in two-dimensional space, increase to values close to 1.7. The invertible treatment with cytochalasin, which destroys the system of the actin filaments, suppresses the normal control of biomineralization and causes a complex form of spicules, the fractal dimension of which varies within 1.5–1.6. Thus, the determination of the fractal dimension value serves as evidence of the fractional essence of the patterns studied, quantifies the spatially complex organization of cells and their assemblies during morphogenesis, and allows us to estimate the variation in the spicule morphology after cytochalasin treatment. 相似文献