Ancient Egyptian verbal reduplication: typology, diachrony, and the morphology–syntax interface

We explore the diachronic development of verbal reduplication in Ancient Egyptian (Afro-Asiatic) by systematically comparing reduplicative stem formations in Old Egyptian (2500–2000 BC) and in Coptic Egyptian (3rd–12th c. AD). Old Egyptian is a language with a rich inventory of reduplication patterns, which have been reduced in the course of almost four thousand years of uninterrupted language history. Coptic retains only a limited number of reduplicative stem formations, frequently with concomitant loss of the simple stem. We show that the decreasing productivity of verbal reduplication in the language correlates with broad morphological and syntactic changes in the verbal domain.     

Reduplication in Slavic and Baltic: loss and renewal

The exposition comprises three parts. Part 1 surveys the Indo-European reduplication patterns (RPs) that have been lost in Slavic and Baltic: the languages show little or no evidence of inherited RPs in present and perfect formations, and in intensive verbs (Sect. 2.1), but some vestiges of reduplication in nouns can be identified (Sect. 2.2). Part 2 describes innovated RPs in the Slavic verb; they can be posited on the basis of scanty evidence that has survived the Late Common Slavic loss of coda obstruents. Part 3 describes Baltic innovations reflected in Lithuanian: reduplicative root formations (Sect. 4.1), several minor lexicalized RPs (Sect. 4.2.), and the para-lexical part of speech called eventives (Sect. 4.3). Their content categories, types of expression, patterns of iconicity, and grammatical function are analysed and exemplified in some detail. It is suggested that formations such as these may form the natural background for the creation of new patterns of morphological expression including patterns of reduplication.     

Convenient experimental determination of adsorption isotherms in a Hydrophobic Interaction Chromatography system

Summary HPLC was combined with a packable microbore guard column to obtain the adsorption isotherm of lysozyme in a Hydrophobic Interaction Chromatography system. The equipment configuration enabled isotherm determination of the protein on a relatively low pressure chromatographic media (TosoHaas 650M Phenyl).Notation C_m,i is the mobile phase concentration of protein. (M/L³ _(liquid)) - C_m,0 =0 - C_s,i is the stationary phase concentration of protein. It is the concentration of protein on the chromatographic media. (M/L³ _(solid)) - C_s,0 =0 - M,L is the dimensions mass and length - V_r,i is the retention volume of the peak front that corresponds to a mobile phase protein on the concentration C_m,i. (L³ _(liquid)) - i i is a counter that is used to keep track of C_m, C_s, and V_r.For example, i=1 in the term C_m,i denotes the first, and lowest, mobile phase protein concentrations are described by higher values of i. - V_d is the system dead volume. It consists of all of the system volume that the mobile phase "sees" or contacts, includingchromatographic media interparticle and pore volume. (L³ _liquid) - V_s the stationary phase volume. V_s is the nonporous bead volume. For porous beads, V_s is the bead volume - the porevolume. (L³ _(solid)) - V_e is the empty column volume. (L³ _liquid) - V_m is the packed column mobile phase volume and consists of the pore volume and the excluded volume. (L³ _(liquid)) - V_{e system} is the empty column system volume. (L³ _(liquid)) - V_frit the volume of mobile phase that fills the column frits. (L³ _(liquid)) - V_woc the system volume without the column connected. (L³ _(liquid))     

The natural history of verb-stem reduplication in Bantu

In this study I present a comparative and historical analysis of “frequentative” Bantu verb-stem reduplication, many of whose variants have been described for a number of Eastern and Southern Bantu languages. While some languages have full-stem compounding, where the stem consists of the verb root plus any and all suffixes, others restrict the reduplicant to two syllables. Two questions are addressed: (i) What was the original nature of reduplication in Proto-Bantu? (ii) What diachronic processes have led to the observed variation? I first consider evidence that the frequentative began as full-stem reduplication, which then became restricted either morphologically (by excluding inflectional and ultimately derivational suffixes) and/or phonologically (by imposing a bisyllabic maximum size constraint). I then turn to the opposite hypothesis and consider evidence and motivations for a conflicting tendency to rebuild full-stem reduplication from the partial reduplicant. I end by attempting to explain why the partial reduplicant is almost always preposed to the fuller base.     

Detection of two allotype-(Ig-1)-linked minor histocompatibility loci by the use ofH-2-restricted cytotoxic lymphocytes in congenic mice

Cytotoxic lymphocytes (CTL) were generated betweenIg-1-congenic strains BALB/c(H-2^d,Ig-1^a) andC.B-17(H-2^d,Ig-1^b) by cross-immunization in both directions and rechallenge in vitro. The effector cell populations specifically lysed target cells sharing both theH-2 haplotype and theIg-1 allele of the sensitizing strain. B- and T-cell blasts were equally good targets, suggesting thatH-2-restricted cytotoxic lymphocytes are not directed against serologically defined conventional allotypic determinants, but probably against minor histocompatibility antigens controlled by genes linked to theIg-1 complex. Competition experiments using cold target cells from a series ofIg-1^b-congenic strains of the BALB/c background (BAB-14, C.B-17, C.B-26) revealed two not yet described minor histocompatibility loci linked to theIg-1 complex: We could demonstrate that BALB/c anti-C.B-17 effector cells recognize at least two distinct antigenic determinants on C.B-17 target cells, but only one on target cells from BAB-14, which carries a recombinantIg-1 complex. From these results we conclude that one of the minor histocompatibility antigens, designated as H(C_H), is encoded by a gene linked to the heavy-chain constant-region (C_H) genes, whereas the second minor histocompatibility antigen, designated as H(V_H), is coded for by a gene linked to the heavy-chain variable-region (V_H) genes. These two new genetic markers may be useful for further analysis of the mouseIg-1 complex because the analysis of the H(C_H) and H(V_H) genes may facilitate the search for recombinants in that chromosomal region.     

“Templatic backcopying” in Guarijio abbreviated reduplication

Although a wide array of phonological properties seem to backcopy in reduplication, it is an open question whether reduplicative templates can backcopy as well. It has been argued that natural languages do not have reduplicative constructions where the base truncates to match the truncated reduplicant (McCarthy & Prince, 1994; McCarthy & Prince, 1999; Spaelti, 1997; inter alia). In Guarijio Abbreviated Reduplication (Miller, 1996), however, both copies of the reduplicative construction truncate, instantiating the pattern that has been claimed not to exist. This paper argues that the Guarijio case fills this typological gap. Although the data can be given a templatic backcopying analysis, this paper defends a Morphological Doubling Theory (MDT) analysis using cophonologies (Inkelas & Zoll, 2005). In MDT, Guarijio Abbreviated Reduplication results from the parallel imposition of a truncating cophonology in each copy of the reduplicative construction. Guarijio Abbreviated Reduplication is predicted to exist by MDT together with other documented cases of parallel phonological modification in reduplication. I am grateful to many people for helpful comments and suggestions, including Isabel Barreras Aguilar, Laura Downing, Nicholas Fleisher, Andrew Garrett, Jason Haugen, Larry Hyman, Yuni Kim, Teresa McFarland, David Mortensen, Mary Paster, Eric Raimy, and Timothy Thornes as well as the audience of the LSA 2005 Annual Meeting in Oakland. I would like to extend a special thanks to Alan Yu for his detailed comments and suggestions to latter versions of this paper. I am particularly indebted to Sharon Inkelas, for her generous advice, feedback, and numerous discussions throughout the development of this paper. I am also grateful to two anonymous reviewers for their comments and criticisms, and especially to Ingo Plag for his patience and detailed suggestions as editor. All remaining errors and omissions are mine. This study was made possible by fellowships by CONACYT (Consejo Nacional de Ciencia y Tecnología, México), the University of California Institute for Mexico and the United States (UCMEXUS) and Fulbright.     

Complexity,polymorphism, and connectivity of mouse V k gene families

To define the polymorphism and extent of the mouse immunoglobulin kappa (Igk) gene complex, we have analyzed restriction-enzyme digested genomic DNA from 33 inbred strains of mice with labeled DNA probes corresponding to 16 V _x protein groups (1 of them previously undescribed) and the J _k/C _K region (V, variable; J, joining; C, constant). These probes detected between 1 and 25 distinct restriction enzyme fragments (REF) that appeared in up to eight polymorphic patterns, thus defining eight mouse Jgk haplotypes. The investigated portion of the V _A repertoire was estimated to encompass between 60 and 120 discernable V _k gene-containing REFs. In contrast to mouse V _H gene families, several V _k gene families defined by these probes appeared to overlap. This observation has implications for V _k gene analyses by nucleic acid hybridization and raises the possibility that the V _A gene complex is a continuum of related sequences.Abbreviations used in this paper C constant - Ig immunoglobulin - J joining - REF restriction enzyme fragment - RFLP restriction fragment length polymorphism - V variable     

Penicillin recovery from aqueous solutions by continuous foam flotation

Summary Penicillin G recovery is investigated in a continuous flotation column in the presence of different collectors which form a complex with penicillin. The performance of the penicillin recovery was investigated as a function of the mole ratio () of collector-to-penicillin and the aliphatic chain length of the collector. At =1 and low penicillin concentrations (e.g., 20 mg·1^-1), high foam liquid concentrations (680 mg·l^-1), low residue concentrations (12 mg·l^-1) and high penicillin separation (56) can be attained. At =4 the separation increases to 150, and 95% of the penicillin can be recovered.Symbols C_p penicillin concentration in feed (mg·l^-1) - C_R penicillin concentration in outlet liquid (mg·l^-1) - C_S penicillin concentration in foam liquid (mg·l^-1) - C_S/C_P penicillin enrichment (-) - C_S/C_R penicillin separation (-) - % Pen in S penicillin yield in foam liquid (%) - VV}_S foam liquid volume flow (ml·min^-1) - VV}_P feed (ml·min^-1) - VV_{N 2} nitrogen flow rate (ml·s^-1) - temperature     

A CACNA1C Variant Associated with Reduced Voltage-Dependent Inactivation,Increased CaV1.2 Channel Window Current,and Arrhythmogenesis

Mutations in CACNA1C that increase current through the Ca_V1.2 L-type Ca²⁺ channel underlie rare forms of long QT syndrome (LQTS), and Timothy syndrome (TS). We identified a variant in CACNA1C in a male child of Filipino descent with arrhythmias and extracardiac features by candidate gene sequencing and performed functional expression studies to electrophysiologically characterize the effects of the variant on Ca_V1.2 channels. As a baby, the subject developed seizures and displayed developmental delays at 30 months of age. At age 5 years, he displayed a QTc of 520 ms and experienced recurrent VT. Physical exam at 17 years of age was notable for microcephaly, short stature, lower extremity weakness and atrophy with hyperreflexia, spastic diplegia, multiple dental caries and episodes of rhabdomyolysis. Candidate gene sequencing identified a G>C transversion at position 5731 of CACNA1C (rs374528680) predicting a glycine>arginine substitution at residue 1911 (p.G1911R) of Ca_V1.2. The allele frequency of this variant is 0.01 in Malays, but absent in 984 Caucasian alleles and in the 1000 genomes project. In electrophysiological analyses, the variant decreased voltage-dependent inactivation, thus causing a gain of function of Ca_V1.2. We also observed a negative shift of V_1/2 of activation and positive shift of V_1/2 of channel inactivation, resulting in an increase of the window current. Together, these suggest a gain-of-function effect on Ca_V1.2 and suggest increased susceptibility for arrhythmias in certain clinical settings. The p.G1911R variant was also identified in a case of sudden unexplained infant death (SUID), for which an increasing number of clinical observations have demonstrated can be associated with arrhythmogenic mutations in cardiac ion channels. In summary, the combined effects of the CACNA1C variant to diminish voltage-dependent inactivation of Ca_V1.2 and increase window current expand our appreciation of mechanisms by which a gain of function of Ca_V1.2 can contribute to QT prolongation.     

Protein recovery by continuous flotation

Summary Bovine serum albumin (BSA) was recovered from aqueous solutions by foam flotation. The protein concentrations in foam liquid C _S, in feed C _Pand in residue C _Rwere determined. The protein enrichment C _S/C_Pand the separation C _S/C_Ras well as the protein fraction in the foam liquid % BSA and foam liquid volume flow were determined as functions of the medium properties, operational conditions, and equipment parameters as well as concentrations of solid particles. At low protein concentrations in feed (e.g., C _P=40 mg · l^-1), and at 40° C, high performance was attained (C _X=2,000 mg · l^-1, C _R=4.4 mg · l^-1, C _S/C_P=50, C _S/C_R=450, 90% BSA. Continuous foam flotation is an efficient procedure for the recovery of low concentrations of proteins from liquid cultures.Abbreviations BSA bovine serum albumine - C _P protein concentration in feed (mg · l^-1) - C _R protein concentration in residue (mg · l^-1) - C _S protein concentration in foam liquid (mg · l^-1) - C _S/C_R protein separation (-) - C _S/C_P protein enrichment (-) - V _P feed rate (ml · min^-1) - V _R residue flow rate (ml · min^-1) - V _S foam liquid volume flow (ml · min^-1) - N number of theoretical stages in an ideal cascade (-) - temperature (° C) - mean residence time (min)     

Quantitative Stress Responses of the V0V1-ATPase of Higher Plants Detected by Immuno-electron Microscopy

Immuno-electron microscopy of negatively stained isolated tonoplast vesicles was used to quantify stress responses of the H⁺-transporting tonoplast ATPase (V₀V₁-ATPase; EC 3.6.1.1) of the C₃/CAM intermediate Mesembryanthemum crystallinum L. and the C₃ plant Hordeum vulgare L. This approach has the advantage that it relates quantitative adaptations at the level of membrane enzymes directly to membrane area and thus is independent of concomitant changes of relative amounts of other membrane proteins which may perturb conclusions when data are expressed on a tonoplast protein basis. It was shown that in M. crystallinum the amount of V₀V₁-ATPase per unit membrane area increased slightly with ageing and pronouncedly with salinity stress. In H. vulgare under salt stress there was an increase in V₀V₁-ATPase amount only in the highly salt tolerant cv. California Mariout and not in the moderately tolerant cv. Carina. This corroborates conclusions from earlier work, where results were expressed on a protein basis, although this was not to be expected a priori. In all comparative ecophysiological studies using tonoplast vesicles at least some key-point tests with the immunonegative staining technique should be included for the sake of prudence. The data obtained here via immunonegative staining of isolated tonoplast vesicles are in very close agreement with much earlier assessments of area and whole cell-related activities given by measurements of entire isolated vacuoles and morphometric analysis, which further corroborates the suitability of the approaches. The data presented here for the first time allow calculations of the coverage of the tonoplast surface of M. crystallinum with V₀V₁-ATPase holoenzyme protein and with total tonoplast protein, i.e. 1.5 to 2.3 . 10^?15 g V₀V₁-ATPase per μm² and 7.4 to 8.8 . 10^?15 g protein per μm², respectively.     

The Ingenious Structure of Central Rotor Apparatus in VoV1; Key for Both Complex Disassembly and Energy Coupling between V1 and Vo

Vacuolar type rotary H⁺-ATPases (V_oV₁) couple ATP synthesis/hydrolysis by V₁ with proton translocation by V_o via rotation of a central rotor apparatus composed of the V₁-DF rotor shaft, a socket-like V_o-C (eukaryotic V_o-d) and the hydrophobic rotor ring. Reconstitution experiments using subcomplexes revealed a weak binding affinity of V₁-DF to V_o-C despite the fact that torque needs to be transmitted between V₁-DF and V_o-C for the tight energy coupling between V₁ and V_o. Mutation of a short helix at the tip of V₁-DF caused intramolecular uncoupling of V_oV₁, suggesting that proper fitting of the short helix of V₁-D into the socket of V_o-C is required for tight energy coupling between V₁ and V_o. To account for the apparently contradictory properties of the interaction between V₁-DF and V_o-C (weak binding affinity but strict requirement for torque transmission), we propose a model in which the relationship between V₁-DF and V_o-C corresponds to that between a slotted screwdriver and a head of slotted screw. This model is consistent with our previous result in which the central rotor apparatus is not the major factor for the association of V₁ with V_o (Kishikawa and Yokoyama, J Biol Chem. 2012 24597-24603).     

Foam behavior of biological media

Summary The concentration dependence of the foaming of aqueous bovine serum albumin (BSA) solutions with and without salt additives and that of the turbidity temperature, T_T, of p-isononylphenol-10-glycolether in presence of KCl, MgSO₄, or K₄ [Fe(CN)₆] were determined. The differences between the turbidity temperatures of the solutions with and without salt additives were used to calculate the apparent concentration BSA in the salt solutions and to estimate their foaming. The measured and calculated foaminesses agree well.Symbols BSA Bovine Serum Albumin - C concentration - C_BSA concentration of BSA - C_salt salt concentration - C_O actual protein concentration in the absence of a salt - C₁ apparent protein concentration in the presence of a salt - C' NP-10 concentration - k constant in Eq. (4) - T_corr correction for T_TO of the salt-free NP-10 solution - T_T turbidity temperature - V_s equilibrium volume of the foam above the liquid layer - V_tg volumetric gas flow rate - foaminess - NP-10 p-isononylphenol-10-glycolether     

A study of lipid bilayer membrane stability using precise measurements of specific capacitance

A method is described for measuring the specific capacitance (C_m) of lipid bilayer membranes with an estimated experimental error of only 1%. The gross capacitance was measured with an AC Wheatstone bridge and a photographic technique was used to determine the area of thin membrane. The results of measurements on oxidized cholesterol-decane membranes formed in 1 × 10^-2 M KCl show that C_m depends upon temperature, voltage, time, and the age of the bulk membrane solutions. For a freshly thinned membrane (from 5 week old solution), C_m increases exponentially from an initial value of 0.432 ±0.021 (SD) μF/cm² with a time constant of ~15 min. A 100 mv potential applied across the membrane for 10-20 min prior to making measurements eliminated this time dependence and produced final-state membranes. C_m of final-state membranes depends upon applied voltage (V_a) and obeys the equation C_m = C₀ + βV_a² where V_a V_DC + V^rms_AC. C₀ and β depend upon temperature; C₀ decreases linearly with temperature while β increases linearly. At 20°C, C₀ = 0.559 ±0.01 (SD) μF/cm² and β = 0.0123 ±0.0036 (SD) (μF/cm²)/(mv²) and at 34°C, C₀ = 0.472 ±0.01 and β = 0.0382 ±0.0039. These variations in C_m are interpreted as resulting from thickness changes. The possibility that they result from diffuse layer and/or membrane dielectric phenomena is discussed and found to be unlikely. The results are discussed in terms of membrane stability by constructing hypothetical potential energy vs. thickness curves.     

Multiple feature affixation in Seenku plural formation

Nominal plural formation in Seenku involves two surface changes: fronting of the final vowel and raising of the final tone. Diachronically, the plural patterns likely derive from a suffix *- Open image in new window , which has been obscured through the loss of falling sonority diphthongs. By comparing plural formation to other morphophonological processes in Seenku, this paper argues that the plural suffix has been restructured as a featural suffix consisting of two features: the vocalic feature [+front], resulting in vowel fronting, and the tonal feature [+raised], resulting in tone raising. Given the atomic nature of the morphosyntactic feature plural, Seenku plural formation represents a strong case of multiple feature affixation, albeit a case that can be accounted for through Max constraints on feature values (Lombardi 2001, etc.) and Realize-Morpheme (van Oostendorp 2005; Trommer 2012) rather than the constraint Max-Flt argued for by Wolf (2007). A level-ordered approach retaining the vocalic suffix is also considered but is shown to suffer from a number of shortcomings, particularly with respect to tone and a challenging class of nasal stems. In short, this paper explores how the synchronic grammar copes with the vestiges of affixal morphology in a language that has undergone heavy reduction.     

Surprising lack of sensitivity of biochemical limitation of photosynthesis of nine tree species to open‐air experimental warming and reduced rainfall in a southern boreal forest

Photosynthetic biochemical limitation parameters (i.e., V_cmax, J_max and J_max:V_cmax ratio) are sensitive to temperature and water availability, but whether these parameters in cold climate species at biome ecotones are positively or negatively influenced by projected changes in global temperature and water availability remains uncertain. Prior exploration of this question has largely involved greenhouse based short‐term manipulative studies with mixed results in terms of direction and magnitude of responses. To address this question in a more realistic context, we examined the effects of increased temperature and rainfall reduction on the biochemical limitations of photosynthesis using a long‐term chamber‐less manipulative experiment located in northern Minnesota, USA. Nine tree species from the boreal‐temperate ecotone were grown in natural neighborhoods under ambient and elevated (+3.4°C) growing season temperatures and ambient or reduced (≈40% of rainfall removed) summer rainfall. Apparent rubisco carboxylation and RuBP regeneration standardized to 25°C (V_cmax25°C and J_max25°C, respectively) were estimated based on AC_i curves measured in situ over three growing seasons. Our primary objective was to test whether species would downregulate V_cmax25°C and J_max25°C in response to warming and reduced rainfall, with such responses expected to be greatest in species with the coldest and most humid native ranges, respectively. These hypotheses were not supported, as there were no overall main treatment effects on V_cmax25°C or J_max25°C (p > .14). However, J_max:V_cmax ratio decreased significantly with warming (p = .0178), whereas interactions between warming and rainfall reduction on the J_max25°C to V_cmax25°C ratio were not significant. The insensitivity of photosynthetic parameters to warming contrasts with many prior studies done under larger temperature differentials and often fixed daytime temperatures. In sum, plants growing in relatively realistic conditions under naturally varying temperatures and soil moisture levels were remarkably insensitive in terms of their J_max25°C and V_cmax25°C when grown at elevated temperatures, reduced rainfall, or both combined.     

Cell recovery by continuous flotation

Summary Cell recovery by means of continuous flotation of the Hansenula polymorpha cultivation medium without additives was investigated as a function of the cultivation conditions as well as of the flotation equipment construction and flotation operational parameters. The cell enrichment and separation is improved at high liquid residence times, high aeration rates, small bubble sizes, increasing height of the aerated column, and diameter of the foam column. Increasing cell age and cultivation with nitrogen limitation reduce the cell separation.Symbols C_P cell mass concentration in medium g·l^–1 - C_R cell mass concentration in residue g·l^–1 - C_S cell mass concentration in foam liquid g·l^–1 - V equilibrium foam volume cm³ - V gas flow rate through the aerated liquid column cm³·s^–1 - V_F feed rate to the flotation column ml/min - ¹ V _S/V foaminess s - mean liquid residence time in the column s     

EFFECT OF THE SINKING RATE OF TWO DIATOMS (THALASSIOSIRA SPP.) ON UPTAKE FROM LOW CONCENTRATIONS OF PHOSPHATE1

The effect of the sinking rate, or rate of medium flow (φ) on the rate of phosphate incorporation (V) by the planktonic diatoms Thalassiosira fluviatilis Hust. and T. pseudonana Hasle & Heimdal in batch and chemostat cultures was determined by passing medium at defined flow rates (0.5–25.0 mm·min^?1) over algae on membrane filters. At concentrations from 1 to 100 μg phosphorus·l^?1 V, increases with increasing velocity of flow, approaching a maximum value (V_m) as described by the empirical relationship: where K_φ is the sinking rate value when V = 1/2 V_m+ V_o and V_o is the uptake at 0 rate of flow. By comparing uptake at controlled flow with uptake in a vigorously stirred medium, the phosphate concentration in the cell boundary layer can be determined. The sinking rate that reduces the phosphate concentration in the boundary layer to half of nominal concentration in the medium is much lower for the larger T. fluviatilis than for T. pseudonana. For both diatoms, it is inversely related to the nominal concentration.