滇产植物皂素成分的研究——Ⅹ.五指莲的两个新甾体皂甙(1)

从重楼属植物五指莲Paris axialis H.Li.根茎中分离到三个甾体皂甙,经化学降解,质谱,核磁共振谱分析,证明其中两个甙为新的化合物,即偏诺皂甙元-3-O-β-D-葡萄吡喃糖基(1→3)[α-L-鼠李吡喃糖基(1→2)]-β-D-葡萄吡喃糖甙(Ⅰ)和24α-羟基偏诺皂甙元-3-O-β-D-葡萄吡喃糖基(1→3)][α-L-鼠李吡喃糖基(1→2)]-β-D-葡萄吡喃糖(Ⅲ);另一个鉴定为薯芋皂甙元-3-O-β-D-葡萄吡喃糖基(1→3)[α-L-鼠李吡喃糖基(1→2)]-β-D-葡萄吡喃糖甙(Ⅱ)。     

鲜盾叶薯蓣中原始皂甙的分离与鉴定

从盾叶薯蓣(Dioscorea zingiberensis Wright)新鲜根茎的甲醇提取物分离到薯蓣皂甙元棕榈酸酯(diosgenin palmitate)、β-谷甾醇(β-sitosterol)、纤细皂甙(gracillin)、原纤细皂甙(protogracillin)和原盾叶皂甙(protozingiberemissaponin),后者为一新甾体皂甙,结构推定为3-O-{α-L-鼠李吡喃糖(1→3)-[β-D-葡萄吡喃糖(1→2)]-β-D-葡萄吡喃糖}-26-O-{β-D-葡萄吡喃糖}-薯蓣皂甙元(3-O-{α-L-rhamnopyran0syl(1→3)-[β-D-glucopyranosyl(1→2)]-β-D-glucopyranosyl}-26-O-{β-D-glucopyranosyl}-diosgenin)。     

滇重楼地上部分的甾体皂甙

从滇重楼Paris polyphylla Sm. var. yunnanensis (Fr.) H-M.地上部分分离得到3个甾体皂甙,经光谱测定和化学降解证明其化学结构分别为:偏诺皂甙元3O-α-L-鼠李吡喃糖基(1→2)〔α-L-鼠李吡喃糖基(1→4))-β-D-葡萄吡喃糖甙(A);孕甾-5,16-二烯-3β-醇-20-酮,3β-O-α-L-鼠李吡喃糖基(1→2)〔α-L-鼠李吡喃糖基(1→4)〕-β-D-葡萄吡喃糖甙(B);孕甾-5,16-二烯-3β-醇-20-酮,3β-O-α-L-鼠李吡喃糖基(1→2)〔α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→4)〕-β-D-葡萄吡喃糖甙(C)。甙A、B和C在滇重楼根中尚未发现,甙C系首次从重楼属植物中获得,而甙A具有止血的活性。     

滇产植物皂素成分的研究——Ⅵ.滇重楼皂甙(2)

从滇重楼干根粉中分离出三个甾体皂甙,鉴定为薯芋皂甙元-3-O-α-L-鼠李吡喃糖(1→2)-β-D-葡萄吡喃糖甙V,偏诺皂甙元-3-O-α-L-鼠李吡喃糖(1→2)-β-D-葡萄吡喃糖甙Ⅵ,偏诺皂甙元-3-O-α-L-鼠李吡哺糖(1→4)-α-L-鼠李吡喃糖(1→4)-〔α-L-鼠李吡喃糖(1→2)〕-β-D-葡萄吡喃糖甙Ⅶ.皂甙Ⅵ和Ⅶ已显示出有趣的生理活性。     

滇产植物皂素成分的研究——Ⅷ.禄劝花叶重接的甾体皂甙

从禄劝花叶重楼Paris Luquanensis H.Li.的干燥根中分离鉴定了二个甾体皂甙:甙Ⅰ称重楼甙H,其化学结构为偏诺皂甙元-3-O-α-阿拉伯呋喃糖(1←4)-[α-L-鼠李吡喃糖(1→2)]-β-D-葡萄吡喃糖甙;甙Ⅱ为偏诺皂甙元-3-O-α-L-鼠李吡喃糖(1→4)-α-L-鼠李吡喃糖(1→4)-α-L-鼠李吡喃糖(1→2)]-β-D-葡萄吡喃糖甙,即我们从滇重楼中分得的重楼甙Ⅶ。二者均具有很有趣的生理活性。此外,从该种植物中还分得胡萝卜甙及β-蜕皮激素。     

小花棘豆化学成分的研究

从有毒植物小花棘豆(Oxytropis glabra DC.)的地上部分分离得到10种化合物,经光谱分析及理化常数测定,分别鉴定为槲皮素(Ⅰ)、山奈酚(Ⅱ)、3′,7-二羟基-2′,4′-二甲氧基-异黄烷(Ⅲ)、山奈-7-O-α-L-鼠李吡喃糖甙(Ⅳ)、山奈酚-3-O-β-D-葡萄吡喃糖甙(Ⅴ)、山奈酚--O-β-D-葡萄吡喃糖(1→2)-β-D-葡萄吡喃糖甙(Ⅵ)、山奈酚-3-O-β-D-葡萄吡喃糖-7-O-β-D-葡萄吡喃糖甙(Ⅶ)槲皮素-3-O-β-D-葡萄吡喃糖甙(Ⅷ)、杨梅树皮甙(Ⅺ)和3-O-[α-L-鼠李吡喃糖基(1→3)-β-D-葡萄吡喃糖基(1→6)-β-D-葡萄吡喃糖醛酸基]-黄豆醇B(Ⅹ)。上述成分均为首次从该植物中分得。化合物Ⅹ为新化合物。     

五指莲重楼的甾体皂甙(2)

从五指莲重楼根中(Paris axialis)分离鉴定了七个甾体皂甙,其中有一新甙Ⅶ,经MS、~1H NMR、~(13)C NMR和化学降解方法证明其化学结构为:24α-羟基偏诺皂甙元-3-O-α-L-鼠李吡喃糖基1→2[α-L-阿拉伯呋喃糖基1→4]-β-D-葡萄吡喃糖甙。     

屏边三七根茎中的两个新的齐墩果酸皂甙

从屏边三七(Panax stipuleanatus Tsai et Feng)根茎中分离到二个齐墩果烷型新皂甙,命名为屏边三七甙(stipuleanoside)R_1和R_2,其化学结构经光谱分析和化学降解反应,分别确定为齐墩果酸-3-O-β-D-葡萄吡喃糖基(1→3)[α-L-阿拉伯呋喃糖基(1→4)]-β-D-葡萄吡喃糖醛酸甙(1)和齐墩果酸[28-O-β-D-葡萄吡喃糖基]-3-O-β-D-葡萄吡喃糖基(1→3)[α-L-阿拉伯呋喃糖基(1→4)]-β-D-葡萄吡喃糖醛酸甙(3)。     

茉莉花的化学成分

从药用植物茉莉花(Jasminum sambac(L．)Ait．)花蕾中分离到9个化合物,通过波谱分析并与已知化合物数据对照,分别鉴定为：苄基-O-β-D-葡萄吡喃糖甙(1),苄基-O-β-D-木吡喃糖基(1→6)-β-D-葡萄吡喃糖甙(2),tetraol(3),molihuaoside D(4),sarnhcoside A(5),sambacoside E(6),芦丁(rutin)(7),山奈酚-3-O-α-L鼠李吡喃糖基(1-2)[α-L鼠李吡喃糖基(1-6)]-β-D半乳吡喃糖甙(8),斛皮素-3-O-α-L鼠李吡喃糖基(1→2)[α-L鼠李吡喃糖基(1→6)]-β-D-半乳吡喃糖甙(9)。     

无患子中两个新的大戟烷型三萜皂甙

从无患子科无患子(Sapindus mukorossi Gaertn.)根中分离到两个新的大戟烷型三萜皂甙Sapimukoside A (1) 和Sapimukoside B (2).运用波谱和化学方法鉴定它们的结构为3-O-α-L-鼠李吡喃糖基-(1→2)-[α-L-阿拉伯吡喃糖基-(1→3)]-β-D-葡萄吡喃糖基-21, 23R-epoxyl tirucall-7, 24R-diene-3β, 21-diol (1) 和3-O-α-L-鼠李吡喃糖基-(1→6)-β-D-葡萄吡喃糖基21, 23R-epoxyl tirucall-7, 24R-diene-3β, 21-diol (2).     

Calcium transport mechanisms in dog red blood cells studied from measurements of initial flux rates

Ca2+ efflux from dog red blood cells loaded with Ca2+ using the A23187 ionophore could be separated into two main components: (1) Mg- and ATP-dependent (active transport) and (2) dependent on external Na (K1/2 around 15 mM); at 80 microM internal free Ca the relative magnitudes of these fluxes were 70% and 30% respectively. The Na-dependent Ca2+ efflux had the following additional properties: (i) it was partially inhibited by ATP depletion or preincubation with vanadate, but it was not affected by Mg2+ depletion; (ii) it failed to be stimulated by external monovalent cations other than Na: (iii) it was stimulated by reduction in the internal Na+ concentration. Both active and Na-dependent Ca2+ efflux remained unchanged in hypotonic solutions or in solutions with alkaline pH (8.5). In cells containing ATP and Mg2+, external Ca2+ inhibited Ca2+ efflux (K1/2 around 1 mM); on the other hand, in Mg-free dog red cells external Ca2+ stimulated Ca2+ efflux (K1/2 about 30 microM). In Mg-depleted red cells incubated in the absence of external Na2+, Ca2+ influx as a function of external Ca2+ followed a monotonically saturable function (K1/2 around 20 microM): addition of Na resulted in (i) inhibition of Ca2+ influx and (ii) a sigmoid relationship between flux and external Ca2+. Intracellular Ca2+ stimulated the external Na-dependent Ca2+ efflux along a sigmoid curve (K1/2 around 30 microM); on the other hand the Ca pump had a biphasic response to internal Ca2+: stimulation at low internal Ca2+ (K1/2 between 1 and 10 microM), followed by a decline at internal Ca2+ concentrations higher than 50 microM.     

Evidence that insulin and concanavalin-A can co-bind to solubilized insulin receptors without inhibiting each other

Concanavalin A (Con A) precipitates detergent-solubilized insulin receptors (free of or bound to [${}^{125}\text{I}$]insulin) prepared from fat cell membranes resulting in a loss of [${}^{125}\text{I}$]insulin binding capacity (or bound hormone) in the soluble fraction. The losses can be recovered by redissolving the precipitates with methyl-α-D-mannoside; the sugar also inhibits precipitation. [${}^{125}\text{I}$]insulin also binds to insoluble Con A-occupied receptors. At all concentrations of Con A tested, the initial amounts of free or hormone-bound receptors were completely accounted for by their distribution between soluble and insoluble states. We conclude that Con A and insulin can co-bind to independent sites on the insulin receptor without inhibiting each other and that previously reported decreases in insulin binding to solubilized insulin receptors were likely due to precipitation by Con A of the receptors.     

Uptake of proteinase-α-macroglobulin complexes by macrophages

Complexes of labelled proteinases (subtilopeptidase A, trypsin) with serum α₁-macroglobulin or α₂-macroglobulin are rapidly taken up in vitro by rabbit alveolar macrophages and peritoneal macrophages but not by mixed rabbit peripheral blood leukocytes. Enzyme, not bound to α₁ - or α₂ -macrogobulin, does not become associated with alveolar macrophages. Chemically inactivated subtilopeptidase A does not bind to α₁ - or α₂ -microglobulin, does not interact with alveolar macrophages. Blocking experiments confirmed that the interaction of proteinase with alveolar macrophages is complex specific; uptake of labelled complex was prevented by the simultaneous addition of macroglobulin complexes formed with non-labelled subtilopeptidase A, subtilopeptidase B, trypsin or chymotrypsin but not by macroglobulin alone. The findings demonstrate a complex-specific interaction between proteinase-α-macroglobulin complexes and macrophages.     

中国链格孢属的研究I.(英)

本文报道链格孢属的2个新种：寄生在苦木科（Simaroubaceae）臭椿［Ailanthusaltissima（Mill.）Swingle]上的臭椿链格孢（Alternariaailanthispnov）,寄生在桦木科（Betulaceas）黑桦（BetuladahuricaPall.）上的桦木链格孢（A．betalaesp．nov．）,2个新组合：豆链格孢［A．azukiae(Hara)comb.nov.],蔷薇生链格孢[A.rosicola（Rao）comb.nov.]和1个新名称红花链格孢（A．carthami-tinctoriinom．nov）。文中为新种提供了拉丁文简介、描述和图。模式标本保藏在山东农业大学植物病理标本室（HSAUP）。     

New Taxa of Chinese Aristolochia L.

Four new species of the genus Aristolochia (Aristolochiaceae) are described as new from China. They are Aristolochia austrochinensis C. Y. Cheng & J. S. Ma, A. caulialata C. Y. Wu, A. salweenensis C. Y. Cheng & J. S. Ma, and A. kunmingensis C. Y. Cheng &J. S. Ma. A naturalized species, A. ringens Vahl is also reported.     

Materials for Chinese Ainsliaea (Compositae)

The genus, Ainsliaea DC. from China is revised in this paper. Three species, A. nana Y. C. Tseng, A. pingbianensis Y. G. Tseng and A. trinervis Y. C. Tseng, are newly described; two species, A. chapaensis Merr. and A. angustifolia Hook. f. et Thoms. ex C. B. Clarke are new records for China and two new combinations, A. apteroides (Chang) Y. C. Tseng and A. macrocephala (Mattf.) Y. C. Tseng, are made. In addition, one species, A. hypoleuca Diels ex Limpr. and four varieties, A. bonatii Beauverd var. arachnoidea Beauverd, A. pteropoda DC. var. leiophylla Franch., A. elegans Hemsl. var. tomentosa Mattf. and A. glabra Hemsl. var. tenuiculis (Mattf.)Chang, are reduced to synonyms.     

3种园蛛幼蛛各龄形态比较

实验室恒温条件下,研究了园蛛属3种园蛛——角园蛛(Araneus cornutus),叶斑园蛛(A.sta),大腹园蛛(A.ventricosus)的各龄幼蛛。描述它们各自的形态特征;指出它们之间的形态差异。     

Clinical relevance of the cagA,vacA and iceA genotypes of Helicobacter pylori in Brazilian clinical isolates

Infection with Helicobacter pylori strains harboring determinants of pathogenicity may lead to a strong inflammatory response in gastric mucosa. In this work, we examined the frequency of the cagA, vacA and iceA genotypes in H. pylori strains isolated from Brazilian patients and correlated these with the clinical manifestations. H. pylori was isolated from 165 patients [30 with non-ulcer dyspepsia cases (NUD); 93 peptic ulcer disease (PUD): 31 gastric ulcers (GU) and 62 duodenal ulcer disease (DU); 18 with erosive gastritis (EG); and 24 gastroesophageal reflux disease (GERD)]. Allelic variants of cagA, vacA and iceA were identified using the polymerase chain reaction. More than one H. pylori strain was detected in 28 cases (17%), and these were excluded from the statistical analysis. We were unable to confirm an association between iceA status and clinical outcome. There was a strong association between the genotype cagA-positive vacA s1 and PUD. However, logistic regression analysis showed that vacA s1 was the only predictive factor for PUD (OR=4.19; 95% CI 1.95-8.98). The presence of the less virulent strain vacA s2 was related to GERD (OR=8.59; 95% CI 2.85-25.91). Our results support the hypothesis that virulent strains may protect against the development of GERD.     

Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus

Endogenous gibberellins (GAs) were extracted and purified from apical buds of Eucalyptus nitens (Deane and Maid.) Maid. and the cambial region of E. globulus (Labill.). then analysed by capillary gas chromatography-mass spectrometry. GA₁ GA₁₉ GA₂₀ and GA₂₉ were identified by full scan mass spectra. Kovats retention indices and high resolution selected ion monitoring. Using deuterated internal standards. GA₁. GA₁₉. GA₂₀ and putative GA₂₉ and GA₅₃ were quantified in the apical buds, while GA₄. GA₈. GA₉ and GA₄₄ were shown to be either absent or present at very low levels. From the cambial region. GA₁ and GA₂₀ were quantified at levels of 0.30 ng (g fresh weight)-¹ and 8.8 ng (g fresh weight)-¹ respectively. These data suggest that the early 13-hydroxylation pathway is the dominant pathway for GA biosynthesis in Eucalyptus .