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1.
The presence of radon in drinking water causes radiation-related health hazards both through inhalation and ingestion. In the present study, 28 drinking water samples from natural flowing springs, freshwater ponds, and deep hand pumps were analyzed in the fault zone of Reasi region of Jammu &; Kashmir. Radon measurement was performed using the RAD7 electronic device for radon content determination. Average mean values of these samples vary from 2.80 ± 0.78 to 74.37 ± 2.76 Bq l ?1. Nineteen drinking water samples analyzed have radon levels in excess of USEPA recommended maximum contamination level of 11.1 Bq l ?1. The annual effective dose from radon in water due to its ingestion and inhalation per individual has also been calculated. Uranium concentration in these water samples was also analyzed for a possible correlation between different types of rocks and values of radon in water. Results obtained have been compared with the results of earlier investigators for mean radon concentration and mean annual effective dose for radon in water from different regions of northern India and Pakistan. It has been found that radon levels in a significant number of water samples collected from the region of fault line are higher than USEPA recommendations. A positive correlation is observed between the depth of the water source and the values of radon levels in water samples collected from these sources. Measurements of radon concentration in these water samples were also performed with a Smart Radon Monitor designed by Bhabha Atomic Research Centre, Trombay, Mumbai, India, for a comparative analysis. 相似文献
2.
A highly γ-ray resistant bacterium, which has not been described hitherto, has been isolated from water containing mud, fur and moss at a radioactive hot spring, Misasa, Tottori Prefecture, Japan, This bacterium was Gram-positive, non-sporulating, pink to red colored and pleomorphic rod at young stage and predominantly coccoid or small short rod at old. The radiosensitivity of this bacterium was lower than that of well-known bacterium Micrococcus radio- durans. When its exponentially growing cells were irradiated in buffer solution aerated sufficiently at room temperature, shoulder dose and D 0 were calculated to be 6 × 10 5 and 1 × 10 6 rads, respectively. The morphological, cultural and physiological characteristics of the bacterium have been studied. These attributes suggested that the organism was a new species, and the name Arthrobacter radiotolerans has been assigned with respect to its high radioresistance. 相似文献
3.
The growth range in nature of bacteria belonging to the genus Thermus was investigated by sampling 55 different hot springs in Iceland. The springs ranged in temperature from 32 to 99°C, and in pH from 2.1 to 10.1. Viable counts of Thermus spp. ranging from 10 to 10 4 CFU/100 ml of spring water were found in 27 of the springs sampled. The temperature range for these bacteria was found to be 55 to 85°C, and the pH range was from about 6.5 to above 10. Thermus spp. were found in springs containing up to 1 mM dissolved sulfide and having conductivity up to 2,000 μS/cm. The distribution of Thermus spp. in a hot spring thermal gradient was also investigated and found to agree well with the overall distribution in individual springs. 相似文献
4.
1. A survey of thirty-two rheocrene springs in central Pennsylvania revealed that, Like Gammarus in lakes and streams, Gammarus minus is absent from springs with pH <6.0 and conductivity <25μS cm ?1 (total range in pH = 4.6–7.7, and in conductivity = 14–411 μS cm ?1). 2. In ten springs G. minus density was positively correlated with Ca 2+ and Mg 2+ hardness, but not with pH, unless three springs with either an exceptionally high velocity or extremely high densities of the potentially competing snail Fontigens nickliniana were omitted. 3. Adults were larger in the springs with few or no large predators than in those with more predators. In all ten springs, adult dry mass was unrelated to spring pH and ionic content, but brooding female dry mass covaried positively with Ca 2+ and Mg 2+ hardness in the five predator-poor springs. 4. Body water, Na and Ca contents and body mass/length ratios varied independently of spring pH and ionic content. Water content was inversely correlated with fat content, but even when expressed as a percentage of fat-free wet mass, it was unrelated to water chemistry. 5. In juveniles, males and non-brooding females, fat content varied independently of spring pH and ionic content, but in brooding females it was correlated with alkalinity and Ca 2+ and Mg 2+ hardness. The cost of reproduction in brooding females may have been a factor here; they had significantly lower per cent fat than did non-brooding females. Juvenile fat content did not differ significantly among spring populations, whereas adult fat content did. The per cent fat of brooding females covaried positively with body size among springs, and this was marginally true for non-brooding females, as well. The residuals of brooding female per cent fat against dry mass were not related to water chemistry. 6. Brood size (number of embryos in a brood) and brood mass varied significantly among populations, but independently of spring pH and ionic content. Both covaried positively with maternal body size among springs. The residuals of these relationships were unrelated to water chemistry, as was the percentage of females brooding. 7. G. minus from a pH 6 spring survived better and lost less body mass in acidic soft water than did those from a pH 7.6 spring. However, although G. minus has apparently been able to adapt (or acclimate) to pH 6 water it has failed to adapt to more acidic waters. A physiological or structural constraint may be involved because this species has probably had ample opportunity to evolve resistance to dilute acidic water. This hypothesis is consistent with the threshold effect observed: above pH 6 G. minus shows very little evidence of osmotic or metabolic stress, but beiow pH 6 viable populations apparently cannot survive at all. However, the gradual linear decrease in population density of G. minus with decreasing alkalinity and Ca 2+ and Mg 2+ hardness suggests that other factors may also be involved (e.g. a decrease in food quality). 相似文献
5.
[目的] 海南海口含有丰富的温泉资源,对温泉微生物多样性进行研究,有助于进一步开发和利用海南温泉微生物资源。[方法] 本文采用Illumina HiSeq高通量测序技术对海口3个温泉[海甸岛荣域温泉(S1)、火山口开心农场温泉(S2)和西海岸海长流温泉(S3)] 水样中微生物ITS序列和16S rRNA基因V3-V4区进行测序及生物信息学分析,探究海口市3个不同区域的温泉真菌多样性与细菌多样性。[结果] (1)α多样性分析表明,真菌群落中,S3 > S1 > S2,而在细菌群落中,S2 > S1 > S3。β多样性分析表明,3个温泉真菌群落和细菌群落组成差异皆显著。(2)分类分析表明,温泉真菌群落优势菌门为子囊菌门( Ascomycota)和担子菌门( Basidiomycota),细菌群落优势菌门为变形菌门( Proteobacteria)、拟杆菌门( Bacteroidetes)、 Thermi、硝化螺旋菌门( Nitrospirae)、绿菌门( Chlorobi)、厚壁菌门( Firmicutes)、绿弯菌门( Chloroflexi)、放线菌门( Actinobacteria)。(3)CCA(Canonical correspondence analysis)分析表明,3个温泉的真菌群落主要影响因子是温度,细菌群落主要影响因子是总磷。[结论] 海南省海口市温泉中含有丰富的微生物资源,其微生物群落组成受多种环境因子影响,且影响真菌和细菌的主要环境因子不同。 相似文献
6.
“Hot spring frog” is an informal name used for the Japanese stream tree frog ( Buergeria japonica), which is widely distributed in Taiwan and the Ryukyu Archipelago in Japan. Some populations of the species are known to inhabit hot springs. However, water temperature can be extremely high around the sources of hot springs. Thus, it is questionable whether B. japonica selectively inhabits such dangerous environments. To address this question, we conducted a series of observations of water temperature preferences of a hot spring population of B. japonica in Kuchinoshima Island in Japan: (a) a field observation of tadpole density in water pools of different temperatures, (b) a field observation of water temperatures where adult males appear for breeding, and (c) an indoor observation of water temperatures selected by adult females for oviposition. As a result, tadpoles showed a higher density in cooler water. Adult males avoided water pools hotter than 37°C, and adult females selected cooler pools for oviposition. Camera records also showed that adult individuals tend to appear around cooler pools. Thus, we did not find any support for the hypothesis that hot spring frogs prefer hot water. Conversely, they apparently tended to prefer cooler water if it was available. Water temperatures around the sources of the hot spring exceed thermal tolerances of the species and could be a strong selective pressure on the population. Thus, the ability to sense and avoid lethal temperatures may be a key ecological and physiological characteristic for the species that inhabit hot springs. 相似文献
7.
Abstract Hot springs are natural environments where hot groundwater comes out from the earth. Exploring the microbial diversity present in hot springs is important first to determine the microorganisms able to proliferate there and to understand their role in biogeochemical cycles. In Algeria, research concerning microbial populations in those ecosystems is limited. This study describes bacterial and archaeal diversity of the ‘Hammam Essalihine’ hot spring in Khenchela province in north-east Algeria using a culture-independent approach. This is the first microbial diversity investigation in the ‘Hammam Essalihine’ hot spring using next-generation sequencing techniques to assess the species classification of thermophilic microorganisms. Genomic DNA was extracted from water samples and the V4–V5 region of 16S rRNA gene were amplified, sequenced, and analyzed. The average temperature of water varies from 68 to 70?°C. High-throughput sequencing analysis revealed the presence of 21 bacterial phyla, including an unknown phylum and distributed across 42 families and 39 genera. The majority of the sequences were observed to belong to the kingdom Bacteria. The bacterial community from this hot spring is dominated by Proteobacteria (41.52%), Chloroflexi (7.62%), and Bacteroidetes (7.62%), whereas the community of Archaea is scarcely present in the study site and the two identified operational taxonomic units (OTUs) are far from what is known in the GenBank database. The study shows several uncharacterized sequences, indicating that the water of ‘ Hammam Essalihine’ hot spring contains undescribed microorganisms. This study is thought to add to the understanding of thermophile diversity and ecology of ‘ Hammam Essalihine’ hot spring. 相似文献
8.
Actinobacterial diversity was investigated in three hot springs [Tengchong Frog Mouth (TFM) hot spring (in Tengchong, China), Kamchatka Robb Flag hot spring (in Kamchatka, Russia), and Nevada Boiling Spring (in Nevada, USA)], a Tengchong Frog Mouth hot spring-derived pond and a soil sample near the TFM hot spring. Culture-independent techniques were employed including denaturing gradient gel electrophoresis and actinobacterial 16S rRNA gene phylogenetic analysis. The three hot springs and the hot spring-derived pond had pH of 6.8 to 7.9 and temperature of 50 to 81°C. Actinobacteria were for the first time found to be very diverse in hot springs with temperature up to 81°C, indicating that they have an unexpected capability of adapting to hot spring environments. A total of 346 actinobacterial clones were obtained from the three hot springs, and these clones were identified as 31 RFLP types, falling into the Actinomycetales (only limit to the Frankineae suborder), the Rubrobacterales, uncultured Candidatus Microthrix, unclassified Actinobacteria, and uncultured Candidate Division OP10. Our data have implications for a better understanding of the distribution of Actinobacteria in hot springs. 相似文献
9.
The need to improve food security in Africa through culture of tilapias has led to transfer of different species from their natural ranges causing negative impacts on wild fish genetic resources. Loboi swamp in Kenya is fed by three hot springs: Lake Bogoria Hotel, Chelaba and Turtle Springs, hosting natural populations of Oreochromis niloticus. The present study aimed at better genetic characterization of these threatened populations. Partial mtDNA sequences of the D-loop region and variations at 16 microsatellite loci were assessed in the three hot spring populations and compared with three other natural populations of O. niloticus in the region. Results obtained indicated that the hot spring populations had mitochondrial and nuclear genetic variability similar to or higher than the large closely related populations. This may be attributed to the perennial nature of the hot springs, which do not depend on rainfall but rather receive permanent water supply from deep aquifers. The study also revealed that gene flow between the three different hot spring populations was sufficiently low thus allowing their differentiation. This differentiation was unexpected considering the very close proximity of the springs to each other. It is possible that the swamp creates a barrier to free movement of fish from one spring to the other thereby diminishing gene flow. Finally, the most surprising and worrying results were that the three hot spring populations are introgressed by mtDNA genes of O. leucostictus, while microsatellite analysis suggested that some nuclear genes may also have crossed the species barrier. It is very likely that the recent intensification of aquaculture activities in the Loboi drainage may be responsible for these introgressions. Taking into account the importance of these new genetic resources, protection and management actions of the Loboi swamp should be accorded top priority to prevent the loss of these spring populations. 相似文献
10.
Carbon monoxide (CO) is one of the common gaseous compounds found in hot volcanic environments. It is known to serve as the
growth substrate for a number of thermophilic prokaryotes, both aerobic and anaerobic. The goal of this work was to study
the process of anaerobic transformation of CO by microbial communities inhabiting natural thermal environments: hot springs
of Uzon Caldera, Kamchatka. The anaerobic microbial community of Treshchinny Spring (80°C, pH 6.5) was found to exhibit two
peaks of affinity for CO ( K
S1 = 54 nM and K
S2 = 1 μM). The actual rate of anaerobic CO transformation by the microbial community of this spring, calculated after obtaining
the concentration dependence curve and extrapolated to the natural concentration of CO dissolved in the hot spring water (20 nM),
was found to be 120 μmol l −1 of sediment day −1. In all the hot springs studied, more than 90% of the carbon of 14CO upon anaerobic incubation was recovered as 14CO 2. From 1 to 5% of 14CO was transformed to volatile fatty acids (VFA). The number of microorganisms capable of anaerobic CO oxidation determined
by dilution-to-extinction method reached 10 6 cells ml −1 of sediment. CO-transforming anaerobic thermophilic microorganisms isolated from the springs under study exhibited hydrogenogenic
type of CO oxidation and belonged to the bacterial genera Carboxydocella and Dictyoglomus. These data suggest a significant role of hydrogenogenic carboxydotrophic prokaryotes in anaerobic CO transformation in Uzon
Caldera hot springs. 相似文献
11.
Microbial communities of Kamchatka Peninsula terrestrial hot springs were studied using molecular, radioisotopic and cultural approaches. Analysis of 16S rRNA gene fragments performed by means of high-throughput sequencing revealed that aerobic autotrophic sulfur-oxidizing bacteria of the genus Sulfurihydrogenibium (phylum Aquificae) dominated in a majority of streamers. Another widely distributed and abundant group was that of anaerobic bacteria of the genus Caldimicrobium (phylum Thermodesulfobacteria). Archaea of the genus Vulcanisaeta were abundant in a high-temperature, slightly acidic hot spring, where they were accompanied by numerous Nanoarchaeota, while the domination of uncultured Thermoplasmataceae A10 was characteristic for moderately thermophilic acidic habitats. The highest rates of inorganic carbon assimilation determined by the in situ incubation of samples in the presence of 14C-labeled bicarbonate were found in oxygen-dependent streamers; in two sediment samples taken from the hottest springs this process, though much weaker, was found to be not dependent on oxygen. The isolation of anaerobic lithoautotrophic prokaryotes from Kamchatka hot springs revealed a wide distribution of the ability for sulfur disproportionation, a new lithoautotrophic process capable to fuel autonomous anaerobic ecosystems. 相似文献
12.
In extreme thermal environments such as hot springs, phages are the only known microbial predators. Here we present the first study of prokaryotic and phage community dynamics in these environments. Phages were abundant in hot springs, reaching concentrations of a million viruses per milliliter. Hot spring phage particles were resistant to shifts to lower temperatures, possibly facilitating DNA transfer out of these extreme environments. The phages were actively produced, with a population turnover time of 1 to 2 days. Phage-mediated microbial mortality was significant, making phage lysis an important component of hot spring microbial food webs. Together, these results show that phages exert an important influence on microbial community structure and energy flow in extreme thermal environments. 相似文献
13.
In extreme thermal environments such as hot springs, phages are the only known microbial predators. Here we present the first study of prokaryotic and phage community dynamics in these environments. Phages were abundant in hot springs, reaching concentrations of a million viruses per milliliter. Hot spring phage particles were resistant to shifts to lower temperatures, possibly facilitating DNA transfer out of these extreme environments. The phages were actively produced, with a population turnover time of 1 to 2 days. Phage-mediated microbial mortality was significant, making phage lysis an important component of hot spring microbial food webs. Together, these results show that phages exert an important influence on microbial community structure and energy flow in extreme thermal environments. 相似文献
14.
Radon is the second leading cause of lung cancer after smoking. Since the previous quantitative risk assessment of indoor radon conducted in France, input data have changed such as, estimates of indoor radon concentrations, lung cancer rates and the prevalence of tobacco consumption. The aim of this work was to update the risk assessment of lung cancer mortality attributable to indoor radon in France using recent risk models and data, improving the consideration of smoking, and providing results at a fine geographical scale. The data used were population data (2012), vital statistics on death from lung cancer (2008–2012), domestic radon exposure from a recent database that combines measurement results of indoor radon concentration and the geogenic radon potential map for France (2015), and smoking prevalence (2010). The risk model used was derived from a European epidemiological study, considering that lung cancer risk increased by 16% per 100 becquerels per cubic meter (Bq/m 3) indoor radon concentration. The estimated number of lung cancer deaths attributable to indoor radon exposure is about 3000 (1000; 5000), which corresponds to about 10% of all lung cancer deaths each year in France. About 33% of lung cancer deaths attributable to radon are due to exposure levels above 100 Bq/m 3. Considering the combined effect of tobacco and radon, the study shows that 75% of estimated radon-attributable lung cancer deaths occur among current smokers, 20% among ex-smokers and 5% among never-smokers. It is concluded that the results of this study, which are based on precise estimates of indoor radon concentrations at finest geographical scale, can serve as a basis for defining French policy against radon risk. 相似文献
15.
Glycerol dialkyl glycerol tetraethers (GDGTs) are core membrane lipids originally thought to be produced mainly by (hyper)thermophilic archaea. Environmental screening of low-temperature environments showed, however, the abundant presence of structurally diverse GDGTs from both bacterial and archaeal sources. In this study, we examined the occurrences and distribution of GDGTs in hot spring environments in Yellowstone National Park with high temperatures (47 to 83°C) and mostly neutral to alkaline pHs. GDGTs with 0 to 4 cyclopentane moieties were dominant in all samples and are likely derived from both (hyper)thermophilic Crenarchaeota and Euryarchaeota. GDGTs with 4 to 8 cyclopentane moieties, likely derived from the crenarchaeotal order Sulfolobales and the euryarchaeotal order Thermoplasmatales, are usually present in much lower abundance, consistent with the relatively high pH values of the hot springs. The relative abundances of cyclopentane-containing GDGTs did not correlate with in situ temperature and pH, suggesting that other environmental and possibly genetic factors play a role as well. Crenarchaeol, a biomarker thought to be specific for nonthermophilic group I Crenarchaeota, was also found in most hot springs, though in relatively low concentrations, i.e., <5% of total GDGTs. Its abundance did not correlate with temperature, as has been reported previously. Instead, the cooccurrence of relatively abundant nonisoprenoid GDGTs thought to be derived from soil bacteria suggests a predominantly allochthonous source for crenarchaeol in these hot spring environments. Finally, the distribution of bacterial branched GDGTs suggests that they may be derived from the geothermally heated soils surrounding the hot springs. 相似文献
16.
Groundwater meiofauna washed out of springs was studied by means of spring water filtration. The principal interest was a quantitative analysis of the number and diversity of outwash fauna in relationship to hydrological conditions. In addition, a comparative analysis was made of outwash fauna of different outlets of the same hydrological system. The spring complex studied is part of the Centraal Plateau in the southern-most part of The Netherlands. This area is characterized by, for The Netherlands, relatively high hydraulic heads and large permeability of aquifers. Influence of human activities (agriculture) in the recharge area was demonstrated by elevated concentrations of nitrate, chloride, and sulphate in the spring waters. No traces of groundwater pollution by heavy metals or pesticides were found. Temporal variation of meiofauna outwash was studied in a single rheocrene spring during one year. This rheocrene had a relatively large and constant discharge (1300±8 ¦h –1). Meiofauna numbers fluctuated between 110–240 ind. m –3. Considerable differences in meiofauna numbers in a one-off analysis of different springs (rheocrenes and helocrenes) were found. Numbers ranged from 140 to 5800 org. m –3 and an inverse relationship with the amount of water discharged was shown. Organisms in the water filtrates were of multiple origin: aquifer (e.g. Niphargus, Parastenocaris), benthic spring head habitats ( e.g. Gammarus, Chironomidae), and (saturated) soils near the outlets ( e.g. Criconomatidae). Meiofauna abundance and composition is discussed in relation to hydrology and spring head morphology. A semi-quantitative analysis of outwash aquifer fauna was masked by the presence of epigean elements or elements of unclear origin. The outwash fauna of rheocrenes of large discharge supposedly is the most representative for the aquifer. Adaptations of hypogean populations to oligotrophic porous environments and the consequences for drift of juvenile stages is discussed. 相似文献
17.
Hot springs integrate hydrologic and geologic processes that vary over short- and long-term time scales. However, the influence of temporal hydrologic and geologic change on hot spring biodiversity is unknown. Here, we coordinated near-weekly, cross-seasonal (~140 days) geochemical and microbial community analyses of three widely studied hot springs with local precipitation data in Yellowstone National Park. One spring (‘HFS’) exhibited statistically significant, coupled microbial and geochemical variation across seasons that was associated with recent precipitation patterns. Two other spring communities, ‘CP’ and ‘DS’, exhibited minimal to no variation across seasons. Variability in the seasonal response of springs is attributed to differences in the timing and extent of aquifer recharge with oxidized near-surface water from precipitation. This influx of oxidized water is associated with changes in community composition, and in particular, the abundances of aerobic sulfide-/sulfur-oxidizers that can acidify waters. During sampling, a new spring formed after a period of heavy precipitation and its successional dynamics were also influenced by surface water recharge. Collectively, these results indicate that changes in short-term hydrology associated with precipitation can impact hot spring geochemistry and microbial biodiversity. These results point to potential susceptibility of certain hot springs and their biodiversity to sustained, longer-term hydrologic changes. 相似文献
18.
Hot environments are between the supporting life extreme niches that appear to have maintained some degree of pristine quality
and of special biotechnological interest. Knowledge on biodiversity in terrestrial hot springs is still scanty and has not
been compared in the light of the specificity of those extreme ecological niches. Study on diversity of thermophilic bacteria
inhabiting a hot spring located in Rupi Basin (RB), South-West Bulgaria, revealed a high phylogenetic richness in it (genotypic
diversity is 0.37). A total of 120 clones were examined, and grouped in 28 phylogenetic types by their RFLP profile. 16S rRNA
gene analysis allowed the identification of nine divisions from the domain Bacteria and one Candidate division. Ten of the retrieved bacterial sequences representing one third of the sequence types showed
less than 97% similarity to the closest neighbor and were referred as new sequences. Four of them were distantly related to
validly described bacteria (showed ≤90% similarity) suggesting new taxons on at least genus level. Comparison of biodiversity
in the spring from Rupi Basin, Bulgaria with that described from other terrestrial hot springs revealed that Proteobacteria, Hydrogenobacter/Aquifex and Thermus are common bacterial groups for terrestrial hot springs. Simultaneously, specific bacterial taxons were observed in different
springs. 相似文献
19.
Thermophilic, aerobic bacteria of the genus Thermus were isolated from submarine alkaline hot springs in Iceland. Five submarine hot springs were sampled, and all had viable counts of Thermus spp. of about 10 3 CFU/ml. All submarine strains grew in the presence of NaCl at 3% or higher, but no strains from terrestrial hot springs would grow at concentrations higher than 1% NaCl. The growth rate of submarine Thermus strains was not stimulated by NaCl and was reduced at NaCl concentrations higher than 1%. The pattern of growth of these isolates on single carbon sources was similar to that of terrestrial isolates. 相似文献
20.
In the Gastein valley, Austria, radon-rich thermal water and air have been used for decades for the treatment of various diseases.
To explore the exposure pathway of radon progeny adsorbed to the skin, progeny activities on the skin of patients exposed
to thermal water (in a bathtub) and hot vapour (in a vapour chamber) were measured by alpha spectrometry. Average total alpha
activities on the patients’ skin varied from 1.2 to 4.1 Bq/cm 2 in the bathtub, and from 1.1 to 2.6 Bq/cm 2 in the vapour bath. Water pH-value and ion concentration did affect radon progeny adsorption on the skin, whereas skin greasiness
and blood circulation did not. Measurements of the penetration of deposited radon progeny into the skin revealed a roughly
exponential activity distribution in the upper layers of the skin. Based on the radon progeny surface activity concentrations
and their depth distributions, equivalent doses to different layers of the skin, in particular to the Langerhans cells located
in the epidermis, ranged from 0.12 mSv in the thermal bath to 0.33 mSv in the vapour bath, exceeding equivalent doses to the
inner organs (kidneys) by inhaled radon and progeny by about a factor 3, except for the lung, which receives the highest doses
via inhalation. These results suggest that radon progeny attachment on skin surfaces may play a major role in the dosimetry
for both thermal water and hot vapour treatment schemes. 相似文献
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