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Tomasz Lipniacki Krzysztof Puszynski Pawel Paszek Allan R Brasier Marek Kimmel 《BMC bioinformatics》2007,8(1):376
Background
The NF-κB regulatory network controls innate immune response by transducing variety of pathogen-derived and cytokine stimuli into well defined single-cell gene regulatory events. 相似文献3.
In this study, trichostatin A (TSA), a histone deacetylase inhibitor, increased the Bone morphogenetic protein-2 (BMP-2) mRNA level in a human osteoblasts line. Deletion analysis of the promoter region revealed that TSA-induced luciferase was
regulated by the BMP-2 promoter spanning from −320 to −310. Electrophoresis mobility shift assay (EMSA) and Chromatin immunoprecipitation (CHIP)
assay proved that this position was a nuclear factor (NF)-κB responsive element. The results above demonstrated that acetylation
plays a crucial role in BMP-2 expression, and acetylation of NF-κB p65 and p50 subunits by TSA treatment may activate the BMP-2 promoter.
Published in Russian in Molekulyarnaya Biologiya, 2008, Vol. 42, No. 6, pp. 984–989.
The text was submitted by the authors in English. 相似文献
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Background
The presence of β-lactamases in Y. enterocolitica has been reported to vary with serovars, biovars and geographical origin of the isolates. An understanding of the β-lactamases in other related species is important for an overall perception of antibiotic resistance in yersiniae. The objective of this work was to study the characteristics of β-lactamases and their genes in strains of Y. intermedia and Y. frederiksenii, isolated from clinical and non-clinical sources in India. 相似文献5.
Fu RH Hran HJ Chu CL Huang CM Liu SP Wang YC Lin YH Shyu WC Lin SZ 《Biotechnology letters》2011,33(5):903-910
Modulation of dendritic cell (DC) fate and function may be one approach for the treatment of inflammatory and autoimmune diseases.
n-Butylidenephthalide (BP), derived from Angelica sinensis, at 40 μg/ml significantly decreased the secretion of interleukin-6 and tumor necrosis factor-α by lipopolysaccharide (LPS)-stimulated
activation of cultured murine DC2.4 cells (P < 0.01). LPS-induced major histocompatibility complex class II (P < 0.05), CD86 (P < 0.01) and CD40 (P < 0.01) expression on DC2.4 cells was also inhibited by BP. The endocytic capacity of LPS-stimulated DC2.4 cells was increased
by BP (P < 0.01). The antigen-presenting capacity of LPS-stimulated DC2.4 cells was decreased by BP (P < 0.05). Moreover, we confirmed BP attenuates the responses of LPS-stimulated activation of DCs via suppression of NF-κB-dependent
pathways. 相似文献
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Johannes F.-W. Greiner Janine Müller Marie-Theres Zeuner Stefan Hauser Thorsten Seidel Christin Klenke Lena-Marie Grunwald Timo Schomann Darius Widera Holger Sudhoff Barbara Kaltschmidt Christian Kaltschmidt 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2013,1833(12):2866-2878
Natural plant-derived products are commonly applied to treat a broad range of human diseases, including cancer as well as chronic and acute airway inflammation. In this regard, the monoterpene oxide 1,8-cineol, the active ingredient of the clinically approved drug Soledum®, is well-established for the therapy of airway diseases, such as chronic sinusitis and bronchitis, chronic obstructive pulmonary disease and bronchial asthma. Although clinical trials underline the beneficial effects of 1,8-cineol in treating inflammatory diseases, the molecular mode of action still remains unclear.Here, we demonstrate for the first time a 1,8-cineol-depending reduction of NF-κB-activity in human cell lines U373 and HeLa upon stimulation using lipopolysaccharides (LPS). Immunocytochemistry further revealed a reduced nuclear translocation of NF-κB p65, while qPCR and western blot analyses showed strongly attenuated expression of NF-κB target genes. Treatment with 1,8-cineol further led to increased protein levels of IκBα in an IKK-independent matter, while FRET-analyses showed restoring of LPS-associated loss of interaction between NF-κB p65 and IκBα. We likewise observed reduced amounts of phosphorylated c-Jun N-terminal kinase 1/2 protein in U373 cells after exposure to 1,8-cineol. In addition, 1,8-cineol led to decreased amount of nuclear NF-κB p65 and reduction of its target gene IκBα at protein level in human peripheral blood mononuclear cells.Our findings suggest a novel mode of action of 1,8-cineol through inhibition of nuclear NF-κB p65 translocation via IκBα resulting in decreased levels of proinflammatory NF-κB target genes and may therefore broaden the field of clinical application of this natural drug for treating inflammatory diseases. 相似文献
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Molecular genetic analysis of melibiose-fermenting Saccharomyces strains isolated from fermentative processes and natural sources in different world regions was conducted to deduce the evolutionary
diversity of Saccharomyces yeasts and find new α-galactosidase MEL genes. The species S. bayanus, S. mikatae, and S. paradoxus were shown to have a single copy of MEL and not accumulate polymeric genes, unlike some S. cerevisiae populations. The polymeric genes MELp1 and MELp2 were identified in S. paradoxus for the first time. Genes identical by 98.7% are located on the chromosomes X and VI, respectively. Phylogenetic analysis
indicates that MEL genes of the Saccharomyces yeasts are species-specific. 相似文献
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K. Škrlep M. Bergant G. M. De Winter B. Bohanec J. Žel R. Verpoorte F. Van Iren M. Camloh 《Biologia Plantarum》2008,52(2):329-333
Different lines of cell suspension cultures of Taxus × media Rehd. and Taxus floridana Nutt. were cryopreserved with a two-step freezing method using a simple and inexpensive freezing container instead of a programmable
freezer. Four to seven days old suspension cell cultures were precultured in growth medium supplemented with 0.5 M mannitol
for 2 d. The medium was then replaced with cryoprotectant solution (1 M sucrose, 0.5 M glycerol and 0.5 M dimethylsulfoxide)
and the cells incubated on ice for 1 h. Before being plunged into liquid nitrogen, cells were frozen with a cooling rate of
approximately −1 °C per min to −80 °C. The highest post-thaw cell viability was 90 %. The recovery was line dependent. The
cryopreservation procedure did not alter the nuclear DNA content of the cell lines. The results indicate that cryopreservation
of Taxus cell suspension cultures using inexpensive freezing container is possible. 相似文献
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Dana Bernátová 《Biologia》2008,63(2):175-176
The paper brings information on an isolated occurrence and morphological characters of Carex × involuta and C. juncella populations in the Vel’ká Fatra Mts. Their presence has been known neither from the territory of Slovakia nor from the whole
Western Carpathians till now. 相似文献
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Yu Long Min Tao Shaojun Liu Huan Zhong Lin Chen Suifei Tao Yun Liu 《Cell and tissue research》2009,338(1):151-159
Gonadotropin-releasing hormone (GnRH), gonadotropin hormone (GTH), and gonadotropin hormone receptor (GTHR) are the pivotal
signal molecules of the hypothalamic-pituitary-gonad (HPG) axis, which plays a crucial role in regulating gonadal development
in vertebrate. In this study, we comparatively analyze the expression characteristics of Gnrh2, Gthβ, and Gthr in red crucian carp diploids, triploids, and allotetraploids. The expression patterns of these genes are similar in the three
fish ploidy types: the Gnrh2 gene is expressed in midbrains, pituitaries, and gonads; the Gthβ gene is expressed in pituitaries; the Gthr gene is mainly expressed in gonads. These results indicate that the three genes participate in the regulation of gonadal
development. By real-time polymerase chain reaction and in situ hybridization, we find that, among these three fish ploidy
types, the expression level of Gthr in the gonads of triploids is lower than those of diploids and tetraploids; this weakens the combination of GTHR with GTH
released from the pituitary and leads to the sterility of triploids, since the gonad cannot produce enough sex steroids. In
addition, the low expression of Gthr in triploids may affect the down-regulation of Gthβ, which then affects the down-regulation of Gnrh2; hence, the expression levels of Gnrh2 and Gthβ genes in triploids are the highest after the breeding season. In conclusion, the differential expression of Gnrh2, Gthβ, and Gthr in triploids and tetraploids is related to their sterility and bisexual fertility, respectively. 相似文献
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Four stereoisomers of 2-norbornyl-N–n-butylcarbamates are characterized as the pseudo substrate inhibitors of cholesterol esterase. Cholesterol esterase shows
enantioselective inhibition for enantiomers of exo- and endo-2-norbornyl-N–n-butylcarbamates. For the inhibitions by (R)-(+)- and (S)-(−)-exo-2-norbornyl-N–n-butylcarbamates, the R-enantiomer is 6.8 times more potent than the S-enantiomer. For the inhibitions by (R)-(+)- and (S)-(−)-endo-2-norbornyl-N–n-butyl-carbamates, the S-enantiomer is 4.6 times more potent than the R-enantiomer. The enzyme-inhibitor complex models have been proposed to explain these different enantioselectivities. 相似文献
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Nathaniel Liddy Peter E. Molloy Alan D. Bennett Jonathan M. Boulter Bent K. Jakobsen Yi Li 《Molecular biotechnology》2010,45(2):140-149
Previously, we have described the use of phage display to generate high affinity disulfide bond-linked T cell receptors (TCRs).
The affinities of the mutant TCRs were analysed after refolding of separately expressed α and β chains from Escherichia coli inclusion bodies. This approach is only suitable for the analysis of small numbers of TCR variants. An attractive alternative
would be soluble expression within the bacterial periplasm, but the generic production of TCRs within the E. coli periplasm has so far not proved successful. Here we show that functional, soluble TCR can be produced within the cytoplasm
of trxB gor mutant E. coli strains, with maximum yields of 3.4 mg/l. We also investigated the effect of coexpressing the folding modulators Skp and
DsbC finding that the TCR expression levels were largely unaffected by these chaperones. Importantly, we demonstrated that
the amount of protein purified from 50 ml starter cultures was sufficient to show functionality of the TCR by specific antigen
binding in both ELISA and surface plasmon resonance (SPR) assays. This TCR production method has the potential to allow rapid
and medium throughput analysis of affinity-matured TCRs selected from TCR phage display libraries. 相似文献
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G. I. Naumov M. Yu. Shalamitskiy E. S. Naumova 《Doklady. Biochemistry and biophysics》2016,467(1):89-91
Using yeast genome databases and literature data, we have conducted a phylogenetic analysis of pectinase PGU genes from Saccharomyces strains assigned to the biological species S. arboricola, S. bayanus (var. uvarum), S. cariocanus, S. cerevisiae, S. kudriavzevii, S. mikatae, S. paradoxus, and hybrid taxon S. pastorianus (syn. S. carlsbergensis). Single PGU genes were observed in all Saccharomyces species, except S. bayanus. The superfamily of divergent PGU genes has been documented in S. bayanus var. uvarum for the first time. Chromosomal localization of new PGU1b, PGU2b, and PGU3b genes in the yeast S. bayanus var. uvarum has been determined by molecular karyotyping and Southern hybridization. 相似文献
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Shisheng Chen Yan Guo Jordan Briggs Felix Dubach Shiaoman Chao Wenjun Zhang Matthew N. Rouse Jorge Dubcovsky 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(3):625-635