共查询到20条相似文献,搜索用时 46 毫秒
1.
Nitric oxide (NO) is an important signaling molecule that plays a pivotal role in stress tolerance. To study the role of NO in drought tolerance and elucidate the underlying mechanisms, NO (0 and 100 μM) was applied to drought-treated soybean plants. Drought stress was imposed by PEG (5% (W/V) of PEG 6000. Nitric oxide improved growth of soybean plants under drought as evidenced by enhanced dry weight (30%). Nitric oxide caused a remarkable increase in activities of catalase and superoxide dismutase (SOD) and SOD expression (14.8-fold), which led to a significant decline in malondealdehyde content under drought conditions. Nitric oxide induced proline biosynthesis due to enhancing pyrroline-5- carboxylate synthetase (P5CS) expression (43.66-fold). The growth-promoting effect of NO application in soybean plants was concomitant with change in metabolic profile (phenolic acid and flavonoid compounds). Nitric oxide up-regulated of phenylalanine ammonia-lyase (PAL) expression in drought-treated plants and may influence on the phenylpropanoid production. Nitric oxide increased salicylic acid (SA) content in soybean plants under stress. So, NO and SA are jointly responsible for boosted tolerance to drought stress in soybean plants. The decrease in unsaturated fatty acid through NO application might reflect a reduction in oxidative damage. These results propose a multifaceted contribution of NO through regulation of physiological and metabolic processes in response to drought stress. 相似文献
2.
As a fundamental approach to the problem of amino acid metabolism in soybean, changes in content of twenty-three free amino acids, two acidic peptides, ammonia, ethanolamine, urea and seventeen total amino acids of cotyledon, hypocotyl and root of soybean during germination were determined with an amino acid analyzer. Glycine Max M. var. T201 (non-nodule-forming) and Glycine Max M. var. T202 (nodule-forming) were used for this experiment. The content and composition of free and total amino acids of cotyledon in both T201 and T202 differ from those of other tissues in any stage of germination. However, no significant difference between these two varieties of soybean has been recognized in patterns of free and total amino acids changes during germination.In dry bean and initial stage of germination a relatively large unknown peak appeared and disappeared thereafter when the change in free amino acid content during germination of soybean was analyzed with amino acid analyzer. From various tests on the unknown peak, it became obvious that the peak was consisted of two peptides, γ-glutamyl-tyrosine and γ-glutamylphenylalanine, which were discovered in soybean by Thompson et al. in 1962. The content of these peptides did not change during the first 20 hours of germination, but they decreased rapidly thereafter and disappeared after 70 hours. 相似文献
3.
It was assumed that salt-induced redox changes affect amino acid metabolism in maize ( Zea mays L.), and this influence may be modified by NO. The applied NaCl treatment reduced the fresh weight of shoots and roots. This decrease was smaller after the combined application of NaCl and an NO-donor ((Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate, DETA/NO) in the shoots, while it was greater after simultaneous treatment with NaCl and nitro-l-arginine (l-NNA, inhibitor of NO synthesis) in the roots. The quantum yield efficiency of photosystem II was not influenced by the treatments. NaCl had a significant effect on the redox environment in the leaves as it was shown by the increase in the amount of glutathione disulphide and in the redox potential of the glutathione/glutathione disulphide redox pair. This influence of NaCl was modified by DETA/NO and l-NNA. Pharmacological modification of NO levels affected salt-induced changes in both the total free amino acid content and in the free amino acid composition. NaCl alone increased the concentration of almost all amino acids which effect was strengthened by DETA/NO in the case of Pro. l-NNA treatment resulted in a significant increase in the Ala, Val, Gly and Tyr contents. The Ile, Lys and Val concentrations rose considerably after the combined application of NaCl and DETA/NO compared to NaCl treatment alone in the recovery phase. NaCl also increased the expression of several genes related to the amino acid and antioxidant metabolism, and this effect was modified by DETA/NO. In conclusion, modification of NO levels affected salt-induced, glutathione-dependent redox changes and simultaneously the free amino acid composition and the level of several free amino acids. The observed much higher Pro content in plants treated with both NaCl and DETA/NO during recovery may contribute to the protective effect of NO against salt stress. 相似文献
4.
Water stress was imposed upon soybean plants ( Glycine max L.)grown in a greenhouse by withholding irrigation for 10 daysafter 5 weeks of growth, and the changes under stress in thelevels of free amino acids, free ammonia and protein were determinedin detail. With a decrease in the leaf water potential, theprotein content gradually decreased, whereas the free ammoniacontent was relatively constant. Water stress induced an increasein the levels of free amino acids normally present in proteinsuch as isoleucine, leucine, valine, phenylalanine, glutamineand histidine, indicating that protein hydrolysis occurs understress. Proline accumulated only under severe stress (below1.5 MPa) and attained 0.86% of the dry weight on day10 (2.6 MPa). Asparagine also accumulated only undersevere stress (below 2.0 MPa). The concentration of glutamicacid, alanine, aspartic acid, serine, glycine and arginine remainedvirtually unchanged during the stress period. Total proline(protein-bound+free) first decreased during mild to moderatestress, and then increased over that of the well-irrigated controlplants at severe stress due to a remarkable accumulation offree proline. These findings indicate that some de novo synthesisof proline occurs under severe stress and that the nitrogensource for this proline synthesis may be protein. (Received July 4, 1981; Accepted September 11, 1981) 相似文献
5.
The difference of total and free amino acids and protein extracted from the leaves of four different reed ecotypes growing in Hexi corridor of Gansu Province were investigated. In all of the different reed ecotypes, the content of Asp, Glu, Gly, Leu and Ala in total amino acids were high, while the contents of Ala, Phe, Met and Thr, Pro in total amino acids varied among different reed ecotypes. Albeit Ala, Glu, Asp, Gly and Ser were the chief composition of free amino acids in leaves of all reed ecotypes, but temarkble difference was found in the content of each free amino acid from different reed ecotypes. The content of free Pro in leaves of salt meadow and salt meadow-sand dune transitional zone reed were 3.5 and 1.6 times respectively as much as in leaves of swamp reed. Swamp reed had 11 soluble proteins whereas other three reed ecotypes show that each has 13 soluble proteins. Three “salt adaptation proteins” (66 kD, 40.3 kD, 16.5 kD) were found in leaves of three reed ecotypes with varying degree of salt stress, however, the contents of 3 “salt adaptation protens” showed a negative correlation with the degree of salt stress. There was a large amount of “special protein” (11.7 kD) in leaves of sand dune reeds. These results suggest that the difference in cytogene expression takes a priority basis of adaptation of reed plants to different habitats, while a closer relationship of reeds tolerance to salt or drought stress with Pro accumulation in cells is seen than with the of accumulation stress adaptation protein. 相似文献
6.
Petunias (Petunia hybrida cv. 'Mitchell') accumulate free proline (Pro) under drought-stress conditions. It is therefore believed that Pro acts as an osmoprotectant in plants subjected to drought conditions. Petunia plants were transformed by Delta(1)-pyrroline-5-carboxylate synthetase genes (AtP5CS from Arabidopsis thaliana L. or OsP5CS from Oryza sativa L.). The transgenic plants accumulated Pro and their drought tolerance was tested. The Pro content amounted to 0.57-1.01% of the total amino acids in the transgenic plants, or 1.5-2.6 times that in wild-type plants grown under normal conditions. The transgenic plant lines tolerated 14 d of drought stress, which confirms that both P5CS transgenes had full functionality. Exogenous L-Pro treatment caused the plants to accumulate Pro; plants treated with 5 mM L-Pro accumulated up to 18 times more free Pro than untreated plants. Exogenous L-Pro restricted the growth of wild-type petunias more than that of Arabidopsis plants. The capacity for free Pro accumulation might depend on the plant species. The growth of petunia plants was influenced not only by the Pro concentration in the plants, but by the ratio of the Pro content to the total amino acids, because the growth of the transgenic petunia plants appeared normal. 相似文献
7.
高粱抗旱品种“3197 B”比不抗旱品种“三尺三”在同样渗透胁迫条件下,要累积更多的游离脯氨酸。未受渗透胁迫前,两品种地上部的总氨基氮和游离氨基氮水平相似。氨基酸组成也相同。六种主要氨基酸分别占总的量和游离氨基酸量的50%和65%左右。受渗透胁迫24h后,总氨基氮水平未变,游离氨基氮增加,两品种的变化相似。因而,品种间脯氨酸累积的差异,似乎不因蛋白质或游离氨基酸的氨基酸组分的不同和蛋白质水解或合成受促进或抑制程度的不同而异。受渗透胁迫后,3197B植株内不但脯氨酸绝对量,而且相对量都超过“三尺三”。脯氨酸合成的潜在能力在3197B和“三尺三”之间有差别。 相似文献
8.
Abiotic stresses induce oxidative stress, which modifies the level of several metabolites including amino acids. The redox control of free amino acid profile was monitored in wild‐type and ascorbate or glutathione deficient mutant Arabidopsis thaliana plants before and after hydroponic treatment with various redox agents. Both mutations and treatments modified the size and redox state of the ascorbate (AsA) and/or glutathione (GSH) pools. The total free amino acid content was increased by AsA, GSH and H 2O 2 in all three genotypes and a very large (threefold) increase was observed in the GSH‐deficient pad2‐1 mutant after GSH treatment compared with the untreated wild‐type plants. Addition of GSH reduced the ratio of amino acids belonging to the glutamate family on a large scale and increased the relative amount of non‐proteinogenic amino acids. The latter change was because of the large increase in the content of alpha‐aminoadipate, an inhibitor of glutamatic acid (Glu) transport. Most of the treatments increased the proline (Pro) content, which effect was due to the activation of genes involved in Pro synthesis. Although all studied redox compounds influenced the amount of free amino acids and a mostly positive, very close (r > 0.9) correlation exists between these parameters, a special regulatory role of GSH could be presumed due to its more powerful effect. This may originate from the thiol/disulphide conversion or (de)glutathionylation of enzymes participating in the amino acid metabolism. 相似文献
9.
The relationship between amino acid and sugar export to thephloem was studied in young wheat plants ( Triticum aestivumL. Pro-INTA, Isla Verde) using the EDTA-phloemcollection technique. Plants grown with a 16 h photoperiod showeda rapid decrease in the concentration of sugars and amino acidsin the phloem exudate from the beginning of the dark period.When plants grown with a 16 h photoperiod were kept in the darkfor longer than 8 h the free amino acid content in leaves andexudate (on a dry weight basis) increased continually throughoutthe 72 h of darkness. During the first 24 h of darkness thesugars in the phloem exudate decreased to 30% of the initialvalue, and returned to the control level when plants were returnedto light. When plants grown under low light intensity for 10d were transferred to high light intensity, they showed an increasein leaf sugar content (dry weight basis) after 3 d but therewere no differences in leaf free amino acid content (dry weightbasis) compared to low-light plants. The sugar concentrationin the phloem exudate was increased by higher light intensities,but there was no difference in the amino acid concentrationof the phloem exudate, and thus the amino acid:sugar ratio inthe phloem decreased in the high-light plants. The present resultssuggest that amino acids can be exported to the phloem independentlyof the export of sugars. Copyright 1999 Annals of Botany Company Sugar exudation, amino acid transport, nitrogen, phloem, transport, wheat, Triticum aestivum L. 相似文献
10.
Soybeans provide an excellent source of protein in animal feed. Soybean protein quality can be enhanced by increasing the
concentration of sulfur-containing amino acids. Previous attempts to increase the concentration of sulfur-containing amino
acids through the expression of heterologous proteins have met with limited success. Here, we report a successful strategy
to increase the cysteine content of soybean seed through the overexpression of a key sulfur assimilatory enzyme. We have generated
several transgenic soybean plants that overexpress a cytosolic isoform of O-acetylserine sulfhydrylase (OASS). These transgenic soybean plants exhibit a four- to tenfold increase in OASS activity when
compared with non-transformed wild-type. The OASS activity in the transgenic soybeans was significantly higher at all the
stages of seed development. Unlike the non-transformed soybean plants, there was no marked decrease in the OASS activity even
at later stages of seed development. Overexpression of cytosolic OASS resulted in a 58–74% increase in protein-bound cysteine
levels compared with non-transformed wild-type soybean seeds. A 22–32% increase in the free cysteine levels was also observed
in transgenic soybeans overexpressing OASS. Furthermore, these transgenic soybean plants showed a marked increase in the accumulation
of Bowman–Birk protease inhibitor, a cysteine-rich protein. The overall increase in soybean total cysteine content (both free
and protein-bound) satisfies the recommended levels required for the optimal growth of monogastric animals. 相似文献
11.
2,4-Diaminobutyric acid (A 2bu) may be responsible for the apparent toxicity of flatpea ( Lathyrus sylvestris L.) forage to some livestock. To obtain information relative to environmental regulation of A 2bu, 3-month old flatpea plants, cv. “Lathco”, were subjected to water-deficit stress for 1, 2, and 4 weeks. A 2bu, the most abundant free amino acid in roots, stems, and leaves, increased nearly 100% in roots of stressed plants. Increases in the concentrations of asparagine (Asn), proline (Pro), and arginine (Arg) occurred in roots; Asn, Pro, and 4-aminobutyric acid (Abu) in stems; and Pro and homoserine (Hse) in leaves also occurred in response to drought stress. Proline was a minor constituent of the free amino acid pool, even under water-deficit stress. The distribution of A 2bu and Pro in the stressed plants (roots > stems > leaves) was the reverse of that in plants supplied with adequate water (roots < stems < leaves). As concentrations of Asn and Abu decreased from roots to leaves in control tissues, concentrations of Hse and A 2bu increased in roughly the same proportions. This observation suggests that Abu and Asn may be precursors of A 2bu and Hse, respectively. The increase in A 2bu levels in aerial parts of drought-stressed flatpea plants is probably not sufficient to lower the feed value of the forage. 相似文献
12.
Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up‐regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses. 相似文献
13.
A photoautotrophic soybean suspension culture was used to study free amino acid pools during a subculture cycle. Free amino acid analysis showed that the intracellular concentrations of asparagine, serine, glutamine, and alanine reached peaks of 200, 10, 9 and 7 mM, respectively, at specific times in the 14-day subculture cycle. Asparagine and serine levels peaked at day 14 but glutamine level rose quickly after subculture, peaking at day three and then declined gradually. Roughly similar patterns were found in the conditioned culture medium although the levels were 1000-fold lower than those found in cells. Photoautotrophic (SB-P) and photomixotrophic (SB-M) cultures were quantitatively similar with regard to free asparagine and serine but not glutamine or free ammonia. Heterotrophic (SB-H) cells had 81–85% less free asparagine on day seven than did SB-M or SB-P cells. Hence, similar to the phloem sap of a soybean plant, asparagine, glutamine, alanine and serine were the predominant amino acids in photoautotrophic soybean cell cultures. Varying the amount of total nitrogen in culture medium for two subcultures at 10, 25, 50, and 100% Of normal levels showed that growth was inhibited only at the 10 and 25% levels but that growth on medium containing 50% of the normal nitrogen was as good as that on 100% nitrogen. Moreover, cellular chlorophyll content correlated exceptionally well with initial nitrogen content of the medium. Thus, the photosynthesis of SB-P cells was not limited by chlorophyll content. SB-P cells grown for two subcultures on 10% nitrogen contained very low free amino acid levels and only 1% of the free ammonia levels found in cells growing on a full nitrogen complement.Abbreviations SB-P
photoautotrophic soybean cells (no sucrose, high CO 2, high light)
- SB-M
photomixotrophic soybean cells (1% w/v sucrose, high light)
- SB-H
heterotrophic soybean cells (3% sucrose, dark) 相似文献
14.
This study aimed to elucidate the role played by Enterobacter asburiae KE17 in the growth and metabolism of soybeans during copper (100 μm Cu) and zinc (100 μm Zn) toxicity. When compared to controls, plants grown under Cu and Zn stress exhibited significantly lower growth rates, but inoculation with E. asburiae KE17 increased growth rates of stressed plants. The concentrations of plant hormones (abscisic acid and salicylic acid) and rates of lipid peroxidation were higher in plants under heavy metal stress, while total chlorophyll, carotenoid content and total polyphenol concentration were lower. While the bacterial treatment reduced the abscisic acid and salicylic acid content and lipid peroxidation rate of Cu‐stressed plants, it also increased the concentration of photosynthetic pigments and total polyphenol. Moreover, the heavy metals induced increased accumulation of free amino acids such as aspartic acid, threonine, serine, glycine, alanine, leucine, isoleucine, tyrosine, proline and gamma‐aminobutyric acid, while E. asburiae KE17 significantly reduced concentrations of free amino acids in metal‐affected plants. Co‐treatment with E. asburiae KE17 regulated nutrient uptake by enhancing nitrogen content and inhibiting Cu and Zn accumulation in soybean plants. The results of this study suggest that E. asburiae KE17 mitigates the effects of Cu and Zn stress by reprogramming plant metabolic processes. 相似文献
15.
Biochemical analyses were made on anthers and pistils at various developmental stages of both male-sterne and fertile plants of Taigu wheat. Analyses ineluded total free amino aeids and free proline. The following results were obtained:
1. There was no significant difference between the content of free proline in anthers of male-sterile and fertile plants at reduction division of mierospore mother cells.
2. In anthers with early uninucleate miorospores, the content of free proline of fertile plants was remarkably higher than that of male-sterile plants. It is interesting to note that at this stage the content of free proline in fertile plants rose to 1.65% of the dry weight of the anther, constituting 50% of the total free amino acids, and amounted to 7-fold of that in male-sterile plants. This result is in line with the results obtained with most cytoplasmic malesterile plants reported by other workers, although malesterility in Taigu wheat is controlled by the nueleus.
3. In pistils, at the stages eorresponding to the early uninneleate and the binueleate stages of the pollen, the free proline content of fertile plants was twice as much as that of the male-sterile plants. This differenee disappeared gradually after fertilization.
4. Tile content of total free amino aoids did not fluetuate as much as the free proline content. There was no differenee in anthers of both types of plants during reduction division of mierospore mother cells. In anthers with early uninueleate pollen grains, total free amino acid content of fertile plants exceeded that of male-sterile plant, the difference levelled off at latter stages. In pistils, before fertilization, the content of total free amino acids of the fertile phmts was slightly higher than that of the male-sterile plants. After fertilization t}fis difference was no nmre noticeable. 相似文献
16.
本文对显性单基因控制的太谷核不育小麦不同发育阶段的可育株和不育株的花药及雌蕊内游离肺氨酸和游离总氨基酸的含量进行了分析。结果表明:(1)在小孢子母细胞减数分裂期,不同育性花药之间游离脯氨酸的含量无明显差异,且含量较低。(2)在小孢子单核初期,可育花药内游离脯氨酸的含量显著高于不育花药,是不育花药的7倍,比减数分裂期增加20倍,高达其干重的1.65%,占其游离总氨基酸的50%。(3)在雌蕊中,游离脯氨酸的含量远远低于花药,不同育性植株之间差异不很明显。(4)关于游离总氨基酸的含量,在花药中减数分裂期,不同育性植株之间无明显差异;在小孢子单核初期,可育株高于不育株。在雌蕊中,相应于小孢子单核初期时,可育株稍高于不育株,受精后迅速趋于一致,但整个变化幅度不大。 相似文献
17.
A series of experiments were conducted to characterize the water stress-induced changes in the activities of RuBP carboxylase (RuBPCO) and sucrose phosphate synthase (SPS), photosystem 2 activity, and contents of chlorophylls, carotenoids, starch, sucrose, amino acids, free proline, proteins and nucleic acids in mulberry ( Morus alba L. cv. K-2) leaves. Water stress progressively reduced the activities of RuBPCO and SPS in the leaf extracts, the chlorophyll content, and PS2 activity in isolated chloroplasts. Plants exposed to drought showed lower content of starch and sucrose but higher total sugar content than control plants. While the soluble protein content decreased under water stress, the amino acid content increased. Proline accumulation (2.5-fold) was noticed in stressed leaves. A reduction in the contents of DNA and RNA was observed. Reduced nitrogen content was associated with the reduction in nitrate reductase activity. SDS-PAGE protein profile showed few additional proteins (78 and 92 kDa) in the water stressed plants compared to control plants. 相似文献
18.
沙棘( Hippophae rhamnoides)是重要的雌雄异株人工林防护树种,但对其环境胁迫的性别响应差异研究不足,性别竞争与胁迫因子的交互效应响应特征尚不清楚。为了探讨锰胁迫和性别竞争交互处理下沙棘雌雄植株的生理响应特征和耐受能力,旨在为沙棘修复土壤重金属污染提供实践指导,该文研究了锰胁迫(4 000 mg·kg -1)和3种不同性别组合模式(雌雄、雌雌、雄雄)处理下沙棘的生理响应,分别测定雌雄沙棘叶片中叶绿素、过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、总酚(total phenols, TP)、游离脯氨酸(free proline,Pro)、可溶性糖(soluble sugar,SS)、甜菜碱(glycine betaine, GB)以及锰含量。结果表明:(1)锰胁迫下,在所有竞争组合中,性间竞争的雄株(M/FM)SOD活性最高,而MDA含量与对照相比未有明显升高,表明雄株的抗氧化能力更好,膜氧化损伤程度更小。(2)锰胁迫时M/FM积累了更多的游离脯氨酸,表现出更好的渗透调节能力和耐受能力。(3)交互效应分析显示性别互作和锰胁迫交互处理显著影响了沙棘雌雄叶片的光合色素、抗氧化酶活性和渗透调节能力; 主成分分析显示SOD、POD、MDA、叶绿素b(chlorophyll b, Chlb)、SS、Pro可作为重要的生理响应指示参数。该研究结果对于利用沙棘修复土壤重金属污染可提供一定的参考。 相似文献
20.
Cadmium toxicity has been extensively studied in plants, however its biochemical mechanism of action has not yet been well
established. To fulfil this objective, four-weeks-old soybean nodulated plants were treated with 200 μM Cd 2+ for 48 h. δ-aminolevulinic acid dehydratase (ALA-D, E.C. 4.2.1.24) activity and protein expression, as well as δ-aminolevulinic
acid (ALA) and porphobilinogen (PBG) concentrations were determined in nodules, roots and leaves. In vitro experiments carried
out in leaves were performed using leaf discs to evaluate the oxidant and antioxidant properties of ALA and S-adenosyl- l-methinone (SAM), respectively. Oxidative stress parameters such as thiobarbituric acid reactive substances (TBARS) and GSH
levels as well as superoxide dismutase (SOD, E.C. 1.15.1.1), and guaiacol peroxidase (GPOX, E.C. 1.11.1.7) were also determined.
Cadmium treatment caused 100% inhibition of ALA-D activity in roots and leaves, and 72% inhibition in nodules whereas protein
expression remained unaltered in the three studied tissues. Plants accumulated ALA in nodules (46%), roots (2.5-fold) and
leaves (104%), respect to controls. From in vitro experiments using leaf discs, exposed to ALA or Cd 2+, it was found that TBARS levels were enhanced, while GSH content and SOD and GPOX activities and expressions were diminished.
The protective role of SAM against oxidative stress generated by Cd 2+ and ALA was also demonstrated. Data presented in this paper let us to suggest that accumulation of ALA in nodules, roots
and leaves of soybean plants due to treatment with Cd 2+ is highly responsible for oxidative stress generation in these tissues. 相似文献
|