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1.
A comparative biochemical and radioautographic in vivo study was performed to identify the site of synthesis and route of migration of albumin in the parenchymal liver cell after labeling with leucine-14C or leucine-3H via the portal vein. Free cytoplasmic ribosomes, membrane-bound ribosomes, rough- and smooth-surfaced microsomes, and Golgi membranes were isolated. The purity of the Golgi fraction was examined morphologically and biochemically. After administration of leucine-14C, labeled albumin was extracted, and the sequence of transport was followed from one fraction to the other. Approximately 2 min after the intravenous injection, bound ribosomes displayed a maximal rate of leucine-14C incorporation into albumin. 4 min later, a peak was reached for rough microsomes. Corresponding maximal activities for smooth microsomes were recorded at 15 min, and for the Golgi apparatus at ~20 min. The relative amount of albumin, calculated on a membrane protein basis, was higher in the Golgi fraction than in the microsomes. By radioautography the silver grains were preferentially localized over the rough-surfaced endoplasmic reticulum at the 5 min interval. Apparent activity in the Golgi zone was noted 9 min after the injection; at 15 and 20 min, the majority of the grains were found in this location. Many of the grains associated with the Golgi apparatus were located over Golgi vacuoles containing 300–800 A electron-opaque bodies. It is concluded that albumin is synthesized on bound ribosomes, subsequently is transferred to the cavities of rough-surfaced endoplasmic reticulum, and then undergoes migration to the smooth-surfaced endoplasmic reticulum and the Golgi apparatus. In the latter organelle, albumin can be expected to be segregated together with very low density lipoprotein in vacuoles known to move toward the sinusoidal portion of the cell and release their content to the blood.  相似文献   

2.
Microsomal UDP-glucuronyl transferases (uridine-5′-diphosphate-glucuronate glucuronyl transferase, acceptor unspecific, EC 2.4.1.17) function in detoxification of a wide range of aglycons containing phenolic, alcoholic, carboxylic or amino acceptor groups through formation of water-soluble glucuronic acid derivatives. To localize this activity within a specific cell component, purified rough-surfaced endoplasmic reticulum and Golgi apparatus fractions from rat liver were compared with total homogenates. The UDP-glucuronyl transferase specific activity of the Golgi apparatus fractions was less than or approximately equal to that of the total homogenate whereas this activity was concentrated 3–8 fold in the rough-surfaced endoplasmic reticulum fraction depending upon substrate. We conclude that the rough-surfaced endoplasmic reticulum is the major site of glucuronide formation within the microsome fraction of normal rat livers.  相似文献   

3.
Summary Actively secreting silk gland cells of caddis fly larvae show the following fine structure: a well developed rough-surfaced endoplasmic reticulum, continuity between roughsurfaced and smooth-surfaced endoplasmic reticulum adjacent to the Golgi saccules, dense material (secretion) in the margins of the Golgi saccules, some of which appear in the form of blebs and discrete membane bounded secretion granules; the latter seem to coalesce and migrate to the surface of the cell where they are discharged. Intracisternal granules appear in glands where the secretion cycle has apparently been interrupted. These observations suggest a secretion cycle for the silk glands comparable to that demonstrated by both morphological and experimental methods in certain other protein secreting cells: namely, synthesis by the ribosomes, transport to the Golgi complex through the cisternae of the endoplasmic reticulum, concentration by the Golgi complex and movement of the secretion granules through the cytoplasm to the surface of the cell where they are discharged.This research was supported by grants from the National Institutes of Health (RG-4706, 5479) and the National Science Foundation (G-9879).  相似文献   

4.
The changes occurring in rat hepatocytes during a 5 day period of treatment with phenobarbital were determined by morphometric and biochemical methods, particular attention being paid to the endoplasmic reticulum. The hepatocytic cytoplasm played an overwhelming part in the liver hypertrophy, while the hepatocytic nuclei contributed to only a moderate extent. The endoplasmic reticulum accounted for more than half of the increase in cytoplasmic volume. The increase in the volume and number of hepatocytic nuclei in the course of phenobarbital treatment was associated with changes in the ploidy pattern. Until the 2nd day of treatment both the rough-surfaced endoplasmic reticulum (RER) and the smooth-surfaced endoplasmic reticulum (SER) participated in the increase in volume and surface of the whole endoplasmic reticulum (ER). Subsequently, the values for RER fell again to control levels, whereas those for SER continued to increase, with the result that by the 5th day of treatment the SER constituted the dominant cytoplasmic element. The specific volume of mitochondria and microbodies (peroxisomes) remained constant throughout the duration of the experiment, while that of the dense bodies increased. The specific number of mitochondria and microbodies displayed a significant increase, associated with a decrease in their mean volume. The phenobarbital-induced increase in the phospholipid and cytochrome P-450 content of the microsomes, as well as in the activities of microsomal reduced nicotinamide-adenine dinucleotide phosphate-cytochrome c reductase and N-demethylase, was correlated with the morphometric data on the endoplasmic reticulum.  相似文献   

5.
Abundant membrane-bounded granules, 0.1–0.45 µm in diameter, occur among the elements of the smooth-surfaced endoplasmic reticulum in zona fasciculata and zona reticularis adrenocortical cells of guinea pigs. Acid phosphatase cannot be cytochemically demonstrated in them, and they are therefore distinct from lysosomes. Incubation in medium containing 3,3'-diaminobenzidine results in dense staining of the granules, identifying them as peroxisomes. These small peroxisomes increase in number as fetal adrenocortical cells differentiate, and they appear to arise from dilated regions of endoplasmic reticulum. They maintain interconnections with the smooth endoplasmic reticulum and with one another.  相似文献   

6.
The incorporation of leucine-3H into either ovarian or oocyte proteins occurs throughout vitellogenesis, but is at a maximum during early phases of this process. The labeling of ovarian and oocyte proteins is inhibited with cycloheximide. Oocytes are permeable to actinomycin D, and this drug does not affect the incorporation of amino acids into oocyte proteins but does block oocyte RNA synthesis. By means of both light microscope and high resolution radioautography, it has been demonstrated that the initial incorporation of leucine-3H under both in vitro and in vivo conditions occurs in elements of the rough-surfaced endoplasmic reticulum in the oocyte. Under pulse-chase conditions, the label subsequently becomes associated with intracisternal (precursor yolk) granules now aggregated within the cisternae of the connected smooth-surfaced endoplasmic reticulum. By 7 days, mature yolk globules are extensively labeled. The results of experiments designed to assess the possible contribution of maternal blood proteins to yolk deposition indicate that such a contribution is minimal. It is concluded that the crayfish oocyte is programmed for and capable of synthesizing the massive store of proteinaceous yolk present in the egg at the end of oogenesis.  相似文献   

7.
Summary Electron microscope studies on the oocytes of several crustacean species demonstrate that the protein yolk arises within vesicular and lamellar forms of the rough-surfaced endoplasmic reticulum. The vesicular form of the endoplasmic reticulum may have its origin from a blebbing process of the outer layer of the nuclear envelope. Disc-shaped granules, representing precursor elements of the yolk granules, appear within the vesicular and lamellar profiles of endoplasmic reticulum. Autoradiographic results suggest that the ribosomes attached to the endoplasmic reticulum take part in the biosynthesis of yolk proteins. Numerous disc-shaped granules accumulate within the cisternae of the endoplasmic reticulum, but eventually they undergo a transformation into a finely granular yolk granule. Thus, both the origin and growth of protein yolk granules occur within membranes constituting the endoplasmic reticulum. The results provide evidence that intra-ooplasmic synthesis of yolk protein occurs in these oocytes.This investigation was supported by research grants (HD-00699; GM-09229) and a Career Development Award (GM-11,524) from the National Institutes of Health, U.S. Public Health Service.  相似文献   

8.
Liver microsomes, isolated from rats which had been treated with phenobarbital in vivo, were found to exhibit increased activities of oxidative demethylation and TPNH-cytochrome c reductase and an increased amount of CO-binding pigment. Simultaneous administration of actinomycin D or puromycin abolished the phenobarbital-induced enzyme synthesis. Increased rate of Pi32 incorporation into microsomal phospholipid was the first sign of phenobarbital stimulation and appeared 3 hours after a single injection of this drug. Microsomes were divided into smooth-surfaced and rough-surfaced vesicle fractions. The fraction consisting of smooth-surfaced vesicles exhibited the greatest increase in protein content and oxidative demethylation activity after phenobarbital administration in vivo. Ultrastructural studies revealed that drug treatment also gave rise to proliferation of the endoplasmic reticulum in the hepatic parenchymal cells, first noticed after two phenobarbital injections. The phenobarbital-induced synthesis of the metabolizing enzymes is discussed with special reference to the relationship to the stimulated synthesis of the endoplasmic membranes.  相似文献   

9.
Summary Yoshida sarcoma cells in four days after inoculation have been studied with the electron microscope in thin sections. The mitochondria found attached to the nuclear membrane in the prophase look to serve a removal of nuclear membrane. In the telophase, the mitochondria are not found in the juxtanuclear region of the cytoplasm, while the smooth-surfaced endoplasmic reticulum makes its appearance surrounding the surface of nucleus. These findings suggest that the smooth surfaced endoplasmic reticulum participates directly in the development of the nuclear membrane. Single or double particles 23–60 m in diameter are found at the nuclear terminal of the double layer of endoplasmic reticulum. The development of the nuclear membrane may be ascribed to such structural elements.  相似文献   

10.
Distribution of phytochrome (as Pfr) among membranes from soybean hypocotyls (Glycine max L. cv. Wayne) was determined by the combined techniques of cell fractionation, difference spectrometry, and electron microscopic morphometry. More than 90% of the phytochrome was found in the soluble fraction. With homogenates prepared in the presence or absence of Mg2+, the portion associated with membrane was only 6.5% and 1%, respectively. In the presence of Mg2+, the content of particulate phytochrome correlated with the amount of endoplasmic reticulum with attached ribosomes in the fractions but not with mitochondria or other membranes (including endoplasmic reticulum membranes from which the ribosomes may have been lost during cell fractionation). In the absence of Mg2+, phytochrome was associated with a “heavy” plasma membrane fraction. The phytochrome content was sufficiently low to be accounted for by a contamination of less than 10% by rough-surfaced fragments of endoplasmic reticulum. The findings show association of phytochrome with a particulate fraction enriched in rough-surfaced fragments of endoplasmic reticulum but do not rule out cosedimentation of some unknown or unspecific phytochrome aggregate with this fraction.  相似文献   

11.
Characteristic intracytoplasmic lamellar bodies were found in a malignant gonadal stromal tumor. These bodies consisted of the stacks of up to 200 tubular cisternae arranged in parallel. Each cisterna had a circular section in tangential view and a diameter of about 85 nm. The cisternae on the outermost side of these lamellar bodies tended to be dilated and adorned with ribosomes. The ends of cisternae were often contiguous with rough-surfaced endoplasmic reticulum. The latter feature is also seen in annulate lamellae, but periodically spaced annuli or discontinuities characteristic of annulate lamellae were never observed. Furthermore, fine ribosomal granules resembling a rosary were recognizable along the whole circumference of the outer surface of each cisterna. The unique structure we describe is a cytoplasmic organelle which, like annulate lamellae, is closely associated with the endoplasmic reticulum and is presumed to be related to the genesis of rough-surfaced endoplasmic reticulum in tumor cells.  相似文献   

12.
The intracellular and intramicrosomal distributions of hexose 6-phosphate dehydrogenase were examined in the livers of normal and starved rats before and after phenobarbital treatment. The results have revealed that the administration of phenobarbital (75 mg/kg body weight, 4 days) causes a significant increase of the hexose 6-phosphate dehydrogenase activity only in smooth-surfaced, but not in rough-surfaced endoplasmic reticulum, with concomitant increases of the activities of NADPH-cytochrome c reductase and aminopyrine demethylase. Starvation prior to the phenobarbital treatment enhanced the effect of phenobarbital on the microsomal enzymes.  相似文献   

13.
Summary Interstitial cells of hydra are small undifferentiated cells containing an abundance of free ribosomes and few other cytoplasmic organelles. They are capable of differentiating into epitheliomuscular, digestive, glandular, nerve cells, and cnidoblasts. Developing epitheliomuscular and digestive cells acquire bundles of filaments, 50 Å in diameter, which later are incorporated into the muscular processes. Early gland cells develop an elaborate rough-surfaced endoplasmic reticulum and one or more Golgi apparatus. Secretory granules originate in the Golgi region eventually filling the apex of the cell. Neurons are recognized first by the presence of an elaborate Golgi apparatus, absence of a well-developed endoplasmic reticulum, and later the appearance of cytoplasmic processes. The most striking feature of nematocyst formation by cnidoblasts is the presence of a complex distribution system between protein synthesizing rough-surfaced endoplasmic reticulum and the nematocyst. This system consists of connections between cisternae of the endoplasmic reticulum with smooth Golgi vesicles which in turn are connected to minute tubules, 200 Å in diameter. The tubules extend from the Golgi region around the nematocyst finally entering the limiting membrane of the nematocyst. It is suggested that the interstitial cells of hydra represent a model system for the investigation of many aspects of cell differentiation.This work was supported by grants from the National Cancer Institute (TlCA-5055) and from the National Institute of Arthritis and Metabolic Diseases (AM-03688), National Institutes of Health, Department of Health, Education and Welfare.The author is indebted to Dr. Russell J. Barrnett for his guidance and interest throughout this investigation.  相似文献   

14.
1. Changes in the structure and function of the rough-surfaced endoplasmic reticulum of the rat liver as deduced by electron microscopy, polysome analysis and the amino acid-incorporating activity of microsome fractions have been followed at various time-intervals after a single intraperitoneal dose of the hepatocarcinogen 4-dimethylaminoazobenzene. 2. The earliest effect observed was detachment of polysomes and disorganization and vesiculation of the cisternae of the rough-surfaced endoplasmic reticulum. This occurred after 6hr. 3. Subsequent to this was a phase of polysome disaggregation accompanied by impaired amino acid-incorporating activity by microsomes together with a much enhanced stimulating effect of polyuridylic acid on amino acid uptake. This reached a maximum at 24hr. 4. By 40hr. polysomes had re-formed and the amino acid-incorporating activity, together with the polyuridylic acid effect, were similar to those in controls. 5. It was not until 112hr. that something like the normal structure of the rough-surfaced endoplasmic reticulum was re-established. 6. It is not yet possible to relate these changes specifically with the process of azo-dye carcinogenesis.  相似文献   

15.
The testis of the Japanese red-bellied newt, Cynops pyrrhogaster pyrrhogaster, caught in the middle of May was investigated in order to demonstrate the presence of steroid hormone (SH)-secreting cells, by means of histochemical and electron microscopic techniques. The newt testis is divided into two main parts of spermatogenetic stages. The anterior part is immature and consists of many seminal cysts containing spermatogonia and spermatocytes. The posterior part is mature and made up of many cysts containing mature spermatozoa and glandular tissue. Histochemical studies revealed Δ5-3β-hydroxysteroid dehydrogenase (Δ5-3β-HSD) activity in the pericystic cells of the mature part, weak in those before spermiation, then gradually becoming more intense in the pericystic cells just after spermiation. Activity was most pronounced in the outer layer of cells of the glandular tissue which originate from the pericystic cells. In contrast to this, the Δ5-3β-HSD reaction was entirely negative in the pericystic cells of the immature part, the Sertoli cells, and the inner cells of the glandular tissue originating from the Sertoli cells. Electron microscopic observation of the pericystic cells and glandular tissue gave further information on the histochemical features. The pericystic cells in the immature part are fibroblast-like cells. The cell organelles of these cells are poorly developed except for rough-surfaced endoplasmic reticulum and rod-shaped mitochondria. In the mature part, the fibroblast-like cells are transformed into cells characteristic of SH-secreting cells. In the cytoplasm, the amount of smooth-surfaced endoplasmic reticulum increases and typical globular mitochondria with tubular cristae take the place of the rod-shaped mitochondria. In addition, a number of lipid droplets and lipofuscin granules appear. Most of the fine structures of the outer layer of the cells of the glandular tissue were found to be similar in appearance to those of the SH-secreting cells in mammals and other vertebrates. These results strongly suggest that, as in the other urodeles, the SH-secreting cells in the testis of the Japanese red-bellied newt are the pericystic cells in the mature part and in the outer layer of cells of the glandular tissue.  相似文献   

16.
Summary The Stannius corpuscle of the eel (Anguilla japonica) consists of numerous ovoid or polymorphic lobules separated by loose connective tissue containing blood capillaries. Each lobule is composed of a number of columnar secretory cells, containing numerous secretory granules, and arranged more or less radially. Each secretory granule, spherical, 0.5–1.0 in diameter, and osmiophilic, is surrounded by a limiting membrane derived from the Golgi membrane. The well developed rough-surfaced endoplasmic reticulum is closely associated with the Golgi field. Some Golgi elements might also be intimately associated with the outer nuclear membrane. Numerous glycogen particles are distributed throughout the cytoplasm. The secretory granules which tend to accumulate near the basal part of the cell might be released into the loose connective tissue. From these facts, the corpuscles of Stannius are considered to be protein-secretory endocrine glands without any similarity to the adrenal gland.  相似文献   

17.
Specimens of murine yolk sac prepared for light and electron microscopy at 8, 12, and 18 days of gestational age revealed a changing morphology of the endodermal cells in the visceral layer as gestation progressed. Basophilia increased between 8 and 12 days and decreased slightly between 12 and 18 days. These changes corresponded to the development of a highly organized rough-surfaced endoplasmic reticulum by 12 days and its partial replacement by smooth-surfaced elements by 18 days. Staining of frozen sections with tetrazolium dyes revealed a substrate dependent localization of the staining reaction within the cells and suggested an underlying compartmentalization of metabolic activities. Two types of apical granules were observed on the basis of both cytochemistry and electron microscopy. These granules were most numerous at 12 days of gestational age. The observations suggest that although synthesis and absorption doubtless occur simultaneously throughout gestation, the emphasis is on synthesis during the first half of the period and absorption during the second half. It is suggested that the change in fine structure may be due to the rupture of Reichert's membrane and the subsequent establishment of direct contact between the endodermal cells and the uterine wall.  相似文献   

18.
SYNOPSIS. Young organisms of Tokophrya infusionum starved for several hr, are best suited for a study of the fine structure of this organism including the distribution of its organelles. Acid phosphatase was localized by a combined electron microscopy and cytochemical approach using modified Gomori methods. The enzyme was found in small dense bodies, spheroid vesicles, missile-like bodies, rough-surfaced endoplasmic reticulum, residue and autophagic vacuoles. The small dense bodies are thought to be primary lysosomes since electron micrographs show a) a continuity between the membrane of the rough-surfaced endoplasmic reticulum and that of the dense bodies and b) a connection between the contents of both structures when the dense bodies form from the endoplasmic reticulum.  相似文献   

19.
A technique is described for measuring the incorporation of myo-inositol-2-3H into the lipid of various regions of the guinea pig pancreatic acinar cell by radioautography. Stimulation of enzyme secretion with either pancreozymin or acetylcholine was associated with increased graining in both the basophilic cytoplasm and the nonbasophilic cytoplasm. Kinetic studies suggested that the incorporation of myo-inositol-2-3H was stimulated independently in the two regions. Most of the increment in graining due to stimulation with pancreozymin or acetylcholine plus eserine was abolished if the tissue was extracted with 2:1 chloroform-methanol before radioautography. On chromatography of lipid extracts of pancreas, the only lipid showing a detectable increment in radioactivity on stimulation with pancreozymin was phosphatidylinositol. Thus, essentially all of the increment in graining is likely to be due to increased incorporation of tritium into phosphatidylinositol. These studies, coupled with earlier studies employing differential centrifugation, indicate that on stimulation of enzyme secretion there is increased synthesis of phosphatidylinositol in the rough-surfaced endoplasmic reticulum and in the smooth-surfaced Golgi membranes. The significance of these observations is discussed in connection with membrane circulation presumed to occur in the pancreatic acinar cell on stimulation of protein secretion. It is suggested that the increased synthesis of phosphatidylinositol may be concerned with the formation of new endoplasmic reticulum and possibly Golgi membrane to replace that which is presumably converted to membrane of the zymogen granules during intracellular protein transport.  相似文献   

20.
Summary The effect of a low calcium diet on the ultrastructure of the parathyroid gland in the chick was examined. Two-week-old White Leghorn chicks were fed a low calcium diet (calcium content 0.63%) for two weeks. In these chicks, the parathyroid glands are grossly enlarged. Hypertrophy and hyperplasia of the chief cells are evident. The plasma membranes between adjacent cells are relatively straight but interdigitate in some places. Chief cells contain occasional membrane-limited secretory granules (150–350 m in diameter) and with contents of variable electron density. Secretory granules are distributed randomly but some are closely applied to the plasma membrane. There is an increase in the development of the rough-surfaced endoplasmic reticulum. The Golgi complex is enlarged and consists of cisternae arranged in concentric layers, smooth-surfaced and coated vesicles and condensing vacuoles. Dilatations of the cisternae at several points are observed. Mitochondria and filaments are also encountered. These morphological features suggest that low calcium intake stimulates the synthetic activity of the chief cells of the chick parathyroid.  相似文献   

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