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1.
Auxin-induced elongation of epicotyl segments of azuki bean ( Vigna angularis Ohwi and Ohashi cv. Takara) was suppressed by fucose-binding lectins from Tetragonolobus purpureus Moench and Ulex europaeus L. These lectins also inhibited auxin-induced cell wall loosening (decrease in the minimum stress-relaxation time of the cell walls) of segments. Auxin caused a decrease in molecular mass of xyloglucans extracted with 24% KOH from the cell walls. The lectins inhibited auxin-induced changes in molecular mass of the xyloglucans. The autolytic release of xylose-containing products from the pectinase-treated cell walls was also suppressed by the lectins. Fucose-binding lectins pretreated with fucose exhibited little or no inhibitory effect on auxin-induced elongation, cell wall loosning, or breakdown of xyloglucans. These results support the view that the breakdown of xyloglucans is involved in the cell wall loosening responsible for auxin-induced elongation in dicotyledons.  相似文献   

2.
Hypocotyl cuttings (from 20- and 50-day-old Pinus taeda L. seedlings) rooted readily within 30 days in response to exogenous auxin, while epicotyl cuttings (from 50-day-old seedlings) rarely formed roots within 60 days. Responses to auxin during adventitious rooting included the induction of cell reorganization and cell division, followed by the organization of the root meristem. Explants from the bases of both epicotyl and hypocotyl cuttings readily formed callus tissue in response to a variety of auxins, but did not organize root meristems. Auxin-induced cell division was observed in the cambial region within 4 days, and later spread to the outer cortex at the same rate in both tissues. Cells at locations that would normally form roots in foliated hypocotyl cuttings did not produce callus any differently than those in other parts of the cortex. Therefore, auxin-induced root meristem organization appeared to occur independently of auxin-induced cell reorganization/division. The observation that N-(1-naphthyl)phthalamic acid (NPA) promoted cellular reorganization and callus formation but delayed rooting implies the existence of an auxin signal transduction pathway that is specific to root meristem organization. Attempts to induce root formation in callus or explants without foliage were unsuccessful. Both the cotyledon and epicotyl foliage provided a light-dependent product other than auxin that promoted root meristem formation in hypocotyl cuttings.  相似文献   

3.
Helga Dahlhelm 《Planta》1969,86(3):224-234
Summary The auxin-induced cell elongation and the formation of indoleacetyl-aspartic acid (IAAsp) of pea epicotyl sections and Agrostemma hypocotyl sections are inhibited by heavy water. The formation of IAAsp requires a specific enzyme. The lack of IAAsp in D2O-treated plant tissues may be due to an influence of D2O on the induction or on the synthesis of that enzyme. Treatment of plant sections with synthetic IAAsp has no effect on the growth of the sections in D2O. Indole-3-acetic acid (IAA) increases the incorporation of 32P-orthophosphate into ribosomal and soluble RNA of pea epicotyl sections in H2O but not in D2O. The synthesis of ribosomal RNA is decreased by heavy water.The effects of IAA and D2O on the soluble proteins of pea sections have been studied by PAA-gel electrophoresis. D2O does not change the pattern of protein bands in comparison with the H2O-control, but prevents the probably IAA-induced alteration of the Rf-value of one protein band on the pherogram. It is assumed that the inhibition of auxin-induced reactions in the D2O-medium is due to the stabilizing effect of heavy water on allosteric proteins. The results of this work support the hypothesis that IAA acts as allosteric effector.  相似文献   

4.
Auxin-induced elongation of epicotyl segments of azuki bean ( Vigna angularis Ohwi et Ohashi cv. Takara) was suppressed by a fucose-binding lectin from Tetragonolobus purpureas Moench and by polyclonal antibodies raised against xyloglucan heptasaccharide (Xyl3Glc4) when the cuticle present in the outer surface of epicotyls was abraded. In contrast, elongation of non-abraded segments was not influenced by the lectin or the antibodies. Epicotyl segments, from which the epidermal and the outer cortical cells had been removed, elongated rapidly for 2 h and than only slowly. Auxin slightly stimulated elongation of the inner tissue segments in the phase of slow growth. Neither in the presence nor in the absence of auxin did the lectin or the antibodies affect elongation of the inner tissue segments. The split portions of outer surface-abraded epicotyl segments incubated in buffer extended outward, and auxininhibited this outward bending. The lectin and the antibodies reversed the effect of auxin on bending. The fucose-binding lectin pretreated with fucose or the immunoglobulin fraction obtained from preimmune serum exhibited little or no inhibitory effect on auxin-induced elongation of abraded or split segments. These results support the view that a breakdown of xyloglucans in the epidermal cell walls plays an essential role in auxin-induced elongation in dicotyledons.  相似文献   

5.
Polyclonal antibodies were raised in rabbits against isoprimeverose (Xyl1Glc1), xyloglucan heptasaccharides (Xyl3Glc4), and octasaccharides (Gal1Xyl3Glc4). Antibodies specific for hepta- and octasaccharides suppressed auxin-induced elongation of epicotyl segments of azuki bean (Vigna angularis Ohwi and Ohashi cv Takara). These antibodies also inhibited auxin-induced cell wall loosening (decrease in the minimum stress-relaxation time and the relaxation rate of the cell walls) of azuki segments. However, none of the antibodies influenced auxin-induced elongation or cell wall loosening of coleoptile segments of oat (Avena sativa L. cv Victory). Auxin caused a decrease in molecular mass of xyloglucans in the cell walls of azuki epicotyls and oat coleoptiles. The antibodies inhibited such a change in molecular mass of xyloglucans in both species. Preimmune serum exhibited little or no inhibitory effect on auxin-induced elongation, cell wall loosening, or breakdown of xyloglucans. The results support the view that the breakdown of xyloglucans is associated with the cell wall loosening responsible for auxin-induced elongation in dicotyledons. The view does not appear to be applicable to poaceae, because the inhibition of xyloglucan breakdown by the antibodies did not influence auxin-induced elongation or cell wall loosening of oat coleoptiles.  相似文献   

6.
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8.
Auxin-regulated Wall Loosening and Sustained Growth in Elongation   总被引:18,自引:9,他引:9       下载免费PDF全文
It is proposed that auxin regulates and coordinates both wall loosening and the supply of wall materials in elongation. The tenets of the proposal allowed testable predictions. It was determined that, if the cell walls of Glycine max L. var. Wayne hypocotyl segments are maintained in a loosened state (by excising the segments directly into pH 4 medium), exogenous auxin induced only the second response. It was also predicted and confirmed that elongating systems, e.g. pea epicotyl, with certain early auxin-induced growth kinetics (an initial high non-steady-state rate followed immediately by a drop to a lower steady-state rate) would show a transient second response (in addition to the usual transient first response) when stimulated by pH 4 medium. Finally, it is pointed out that recent results which establish the existence of auxin-induced elongation-associated proteins support the proposition that auxin coordinates wall loosening and the supply of wall materials in elongation.  相似文献   

9.
Summary In epidermal cells of azuki bean (Vigna angularis) epicotyl segments, that were sequentially treated with an auxin-free solution and an auxin solution, cortical microtubules changed their orientation from longitudinal to transverse. Auxin caused the reorientation of microtubules from longitudinal to transverse in segments that were kept under anaerobic conditions and, therefore, showed no elongation, indicating that auxin can regulate the orientation of microtubules by a mechanism that does not involve auxin-induced change in the rate of cell elongation.Abbreviations DMSO dimethylsulfoxide - GA3 gibberellic acid - IAA indoleacetic acid - MT microtubule - PBS phosphate-buffered saline  相似文献   

10.
用饲喂蛋白质和核酸合成的放射性前体[3 H]-Phe、[3 H]-尿嘧啶和[3 H]-胸腺嘧啶证实了油菜素内酯(BR)能促进绿豆上胚轴的生长和蛋白质、RNA 及DNA 的合成。用蛋白质和核酸合成抑制剂(CH、Act.D、5-Fu)进一步探讨它们对上胚轴伸长的抑制作用与蛋白质、RNA、DNA 和m RNA 合成之间的关系。证明了上胚轴的伸长依赖于蛋白质和核酸的合成,尤其是依赖于m RNA 的合成。说明BR是在转录水平上调节基因的表达,进而促进上胚轴的伸长  相似文献   

11.
Calcium has been implicated in various regulatory roles in plantcells including auxin-induced cell elongation. Treatment ofpea epicotyl segments with the calcium chelators, EGTA and chlorotetracycline(CTC), the calcium ionophore, A23187 [GenBank] , and channel blocker, D-600,inhibits auxin-induced cell elongation. Depletion of tissuecalcium either by EGTA or EGTA and a calcium ionophore doesnot interfere with the induction of the early auxin induciblemRNAs pIAA4/5 and pIAA6. Similarly, an increase in cytosoliccalcium with calcium and calcium ionophore neither induces thehormonally regulated mRNAs nor interferes with their inductionby auxin. The calcium channel blocker, D-600, is without effecton the auxin-regulated mRNA induction. The results indicatethat calcium is not involved in the rapid induction of IAA4/5and IAA6 genes in pea tissue. However, a possible role for calciumin the translation of these mRNAs, or in the expression of otherauxin-regulated genes, is not excluded. 3Present Address: Department of Biology, Tokyo MetropolitanUniversity, Tokyo, Japan. (Received April 8, 1988; Accepted July 30, 1988)  相似文献   

12.
Using leaf epidermis from Vicia faba, we tested whether auxin-induced stomatal opening was initiated by auxin-induced ethylene synthesis. Epidermis was dark-incubated in buffered KNO3 containing 0.1 mM alpha-napthalene acetic acid or 1 mM indole-3-acetic acid. Maximum net opening was ca. 4 micron after 6 h. Opening was reversed by 20 microM ABA, 0.1 mM CaCl2. 1-Aminocyclopropane carboxylic acid (ACC) synthase catalyzes synthesis of ACC, the immediate precursor to ethylene. Auxin-induced stomatal opening was fully inhibited by 10 microM 1-aminoethoxyvinylglycine (AVG), an ACC synthase inhibitor. In solutions containing AVG, auxin-induced opening was restored in a concentration-dependent manner by exogenous ACC, but not in control solutions lacking an auxin. ACC-mediated reversal of AVG-inhibition of stomatal opening was inhibited by alpha-aminoisobutyric acid (AIB), an inhibitor of ACC oxidase, the last enzyme in the ethylene biosynthetic pathway, by 10 microM silver thiosulfate (STS), an inhibitor of ethylene action, and by 20 microM ABA, 0.1 mM CaCl2. CoCl2, an inhibitor of ethylene synthesis, also inhibited auxin-induced opening. Both STS and CoCl2 inhibited opening induced by light or by fusicoccin, but neither light- nor fusicoccin-induced opening was inhibited by AVG. These results support the hypothesis that auxin-induced stomatal opening is mediated through auxin-induced ethylene production by guard cells.  相似文献   

13.
We have reinvestigated the role of protein-bound hydroxyproline (extensin) in auxininduced cell enlargement using discs excised from tubers of Solanum tuberosum L. cv. Pentland Crown and from roots of Cichorium intybus L. cv. Magdeburg. Extensin increases markedly in potato tuber discs treated with water and auxin, and the hydroxyproline is primarily in the cell wall. 2,2'-Dipyridyl totally inhibits both hydroxyproline synthesis and auxin-induced cell expansion in potato with the inhibitions being reversed in parallel by Fe2+. Free hydroxyproline also totally prevents induced cell enlargement. Pretreatment with gibberellic acid totally inhibits subsequent auxin-induced cell expansion but does not inhibit hydroxyproline synthesis. Therefore, the level of hydroxyproline does not control auxin-induced cell enlargement in potato tuber discs. Other interpretations are discussed but we conclude that extensin biosynthesis is necessary for auxin-dependent cell expansion as inhibition of the synthesis prevents the induced expansion. Dipyridyl and free hydroxyproline partially inhibit auxin-induced cell enlargement in chicory root discs. Thus a component of the auxin-dependent cell enlargement in chicory is also dependent on extensin synthesis.  相似文献   

14.
There are several differences between monocotyledonous and dicotyledonous plants. The sensitivity towards added galactose which inhibits auxin-induced coleoptile elongation but not stem elongation is one of the conspicuous differences between the two types of plants. InAvena coleoptile segments, galactose, probably as galactose-1-phosphate, inhibits the formation of UDP-glucose from glucose-l-phosphate. The inhibition of UDP-glucose formation due to galactose is not found inPisum epicotyl segments. InAvena UTP: α-D-glucose-1-phosphate uridyltransferase (EC 2.7.7.9) which catalyzes the reaction from glucose-1-phosphate to UDP-glucose seems to be inhibited by galactose-1-phosphate.  相似文献   

15.
Changes in the pattern of protein synthesis and in the translatable mRNA population have been examined during auxin-induced root development from excised mung bean seedlings. Several proteins, predominantly of low molecular weight and high pI, as shown by two-dimensional polyacrylamide gel electrophoresis, are synthesized specifically by auxin-treated tissue. These auxin-induced proteins appear between 6 and 12 hours of auxin treatment, reach a maximum at 24 hours, and decline at 48 hours. Untreated seedlings (placed in Hoagland solution), known to produce small number of roots at the cut end probably due to endogeneous auxin accumulated at the cut end through basipetal transport, show low level synthesis of auxin-specific proteins. Antiauxin treatment that completely inhibits auxin-induced rooting also prevents the appearance of auxin-induced proteins. The induction of a group of three to four proteins appears to be specific to antiauxin treatment. In vitro translation of mRNA from auxin-treated tissue, but not of mRNA from antiauxin-treated tissue, yields several polypeptides of low molecular weight and high pI. Since the auxin-induced proteins precede root development and are synthesized transitorily, it is likely that they play some regulatory role during the initiation of root development. The result show that auxin-induced root formation involves altered gene expression.  相似文献   

16.
The effects of auxin and gibberellic acid on cell wall composition in various regions of epicotyls of azuki bean ( Vigna angularis Ohwi and Ohashi cv. Takara) were investigated with the following results. (1) Young segments excised from apical regions of the epicotyl elongated in response to added 10−4 M indole-3-acetic acid (IAA). When the segments were supplied with 50 m M sucrose, the IAA-induced segment growth was accompanied by enhanced overall synthesis of cell wall polysaccharides, such as xyloglucans, polyuronides and cellulose. This IAA effect on the cell wall synthesis is a consequence of extension growth induced by IAA. Gibberellic acid (GA) at 10−4 M synergistically enhanced the IAA-induced cell wall synthesis as well as IAA-induced extension growth, although GA by itself neither stimulated the cell wall synthesis nor extension growth. In the absence of sucrose, cell wall synthesis was not induced by IAA or GA. (2) In mature segments excised from basal regions of the epicotyl, no extension growth was induced by IAA or GA. GA enhanced the synthesis of xylans and cellulose when the segments were supplied with 50 m M sucrose. IAA had no effect on the cell wall synthesis. These findings indicate that synthesis of polyuronides, xyloglucans and cellulose, which occurs during extension growth of the apical region of the epicotyl, is regulated chiefly by auxin whereas synthesis of xylans and cellulose during cell maturation in the basal region of the epicotyl is regulated by GA.  相似文献   

17.
Galactose inhibits auxin-induced growth of Avena coleoptiles by at least two mechanisms. First, it inhibits auxin-induced H(+)-excretion needed for the initiation of rapid elongation. Galactose cannot be doing so by directly interfering with the ATPase since fusicoccin-induced H(+)-excretion is not affected. Secondly, galactose inhibits long-term auxin-induced growth, even in an acidic (pH 4.5) solution. This may be due to an inhibition of cell wall synthesis. However, galactose does not reduce the capacity of walls to be loosened by H+, given exogenously or excreted in response to fusicoccin.  相似文献   

18.
A linear stress strain analyzer was used to determine the effects of inhibitors of RNA and protein synthesis on auxin-induced increases in cell wall extensibility. With etiolated soybean hypocotyl, maize mesocotyl and Avena coleoptile sections and light-grown pea internode sections, inhibition of RNA synthesis resulted in inhibition of auxin-induced extensibility changes and cell expansion. The results with both actinomycin D and cycloheximide support an earlier conclusion that unstable cell constituents, presumably enzymes, are essential for cell wall loosening induced by auxin as well as for cell elongation.  相似文献   

19.
The effects of kinetin on growth and RNA metabolism in excisedsoybean hypocotyl were investigated, and compared to the effectsof 5-fluorouracil. Kinetin inhibits auxin-induced growth, butnot control growth. RNA synthesis is also inhibited by kinetin,but in a differential fashion. Ribosomal RNA synthesis is almostcompletely inhibited, while TB-RNA synthesis is partially inhibited.D-RNA synthesis is apparently not affected. Base compositionanalysis of these fractions of RNA was carried out. The implicationsfor RNA-mediated auxin-induced growth are discussed. 1The research was supported by NIH grant GM-10157 from the U.S. Public Health Service. 2Purdue University AES Paper No. 3334  相似文献   

20.
Sacher JA 《Plant physiology》1967,42(10):1334-1342
A comparative study was made of the effects of auxin (α-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis amongst the subcellular fractions of sections of endocarp from Kentucky Wonder pole beans (Phaseolus vulgaris, L.). Auxin caused considerable enhancement of incorporation of labeled precursors into RNA and protein of all subcellular fractions, and induced net synthesis of RNA and protein. That auxin-induced net synthesis of protein is repressed by actinomycin D indicates that auxin acts primarily to stimulate synthesis of RNA, as a result of which synthesis of protein is enhanced. The effect of kinetin alone on synthesis of RNA, or of kinetin on auxin-induced synthesis of RNA was variable, with either stimulation or inhibition observed in different experiments. Kinetin-enhancement of synthesis of both RNA and protein in subcellular fractions also varied, with enhancement of synthesis in 1 or all subcellular fractions among different experiments. The variable effect of kinetin did not seem to be related to the amount of endogenous or added auxin. The mode of action of kinetin is discussed.  相似文献   

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