Crystallization and preliminary X-ray data for parvalbumin IIIf of Opsanus tau

The parvalbumin III_f of the superfast swimbladder muscle of Opsanus tau has been crystallized by a vapor diffusion technique using (NH₄)₂SO₄ as a precipitant agent. The crystals belong to space group P2₁, with a unit cell $\text{a = 27.31}\text{A}\text{?}\text{, b = 58.75}\text{A}\text{?}\text{, c = 30.83}\text{A}\text{?}$ and β = 98 ° 30′, and the asymmetric unit contains one molecule. These crystals are suitable for a high resolution crystallographic investigation.     

Manganese superoxide dismutases from Escherichia coli and from yeast mitochondria: Preliminary X-ray crystallographic studies

The Mn superoxide dismutase from Escherichia coli has been obtained in three crystal forms: (I) from 68% saturated (NH₄)₂SO₄, space group P222 or P222₁, $\text{a = 47}\text{A}\text{?}\text{, b = 103}\text{A}\text{?}\text{, c = 47.5}\text{A}\text{?}$, with one subunit per asymmetric unit; (II) from 50% polyethylene glycol 6000, space group C222₁ (with approx. P4₁2₁2 symmetry), $\text{a = 101}\text{A}\text{?}\text{, b = 108}\text{A}\text{?}\text{, c = 180}\text{A}\text{?}$, with four subunits (2 molecules) per asymmetric unit; (III) from 52% polyethylene glycol with a different method of preparing the enzyme solution, space group P2₁2₁2, $\text{a = 47}\text{A}\text{?}\text{, b = 51}\text{A}\text{?}\text{, c = 188}\text{A}\text{?}$, with two subunits per asymmetric unit.The yeast mitochondrial Mn superoxide dismutase has yielded the same crystal form both from 30% 2-methyl-2,4-pentane diol and from 23% polyethylene glycol 6000: space group P2₁2₁2₁, $\text{a = 63}\text{A}\text{?}\text{, b = 115}\text{A}\text{?}\text{, c = 125}\text{A}\text{?}$, with four subunits (one molecule) per asymmetric unit.A full X-ray crystallographic study of at least one of these enzymes is planned.     

Preliminary x-ray study of crystals of mitogenic lectin from pea seeds.

Crystals of mitogenic lectin from pea seeds (Pisum sativum) have been grown. The crystals are in space group P2₁2₁2₁ with unit cell dimensions: $\text{a = 51.0 ± 0.2}\text{A}\text{?}\text{, b = 617 ± 0.2}\text{A}\text{?}\text{, c = 137.6 ± 0.5}\text{A}\text{?}$. The asymmetric unit has one protein molecule.     

Crystallography of Bacillus subtilis levansucrase

Levansucrase, an exocellular enzyme, has been isolated from a high producer mutant, the BS5C4 constitutive strain, of Bacillus subtilis. Three crystalline forms have been obtained, all three belonging to the orthorhombic space group P2₁2₁2₁. The most suitable form for a three-dimensional structure investigation has cell dimensions, $\text{a = 68}\text{A}\text{?}$, $\text{b = 125}\text{A}\text{?}$, $\text{c = 54}\text{A}\text{?}$. There is one molecule in the asymmetric unit.     

Crystal data, molecular dimensions and molecular symmetry in cytochrome oxidase from Pseudomonas aeruginosa.

Pseudomonas aeruginosa cytochrome oxidase (nitrite reductase, cytochrome cd) has been crystallized in space group P2₁2₁2 with cell dimensions $\text{a = 122.8}\text{A}\text{?}\text{, b = 87.2}\text{A}\text{?}\text{, c = 73.4}\text{A}\text{?}$. Density measurements suggest that the asymmetric unit contains one 63,000 molecular weight subunit of the dimeric molecule. Crystal data agree well with electron microscopy of single molecules. The X-ray pattern extends beyond 2.5 Å resolution, and structure analysis is in progress.     

Preliminary crystallographic data for cross-linked (lysine7-lysine41)-ribonuclease A

A cross-linked derivative of ribonuclease A, N^ε,N^ε′-(2,4-dinitrophenylene-1,5)-(lysine⁷-lysine⁴¹)-RNase A, has been crystallized by dialysis against 30% ($\text{v}\text{v}$) ethanol/water mixtures buffered at high pH. Single crystals belong to the orthorhombic space group P2₁2₁2₁, $\text{a = 37.2}\text{A}\text{?}\text{, b = 41.2}\text{A}\text{?}\text{, b = 41.2}\text{A}\text{?}$, with one molecule in the Crystallographic asymmetric unit.     

Preliminary crystallographic data for lysozyme produced by Streptomyces erythraeus.

Crystals of lysozyme from Streptomyces erythraeus are orthorhombic, space group P2₁2₁2₁ with unit cell dimensions: $\text{a = 50.01}\text{A}\text{?}\text{, b = 61.98}\text{A}\text{?}$ and $\text{c = 67.85}\text{A}\text{?}$; and one molecule, of molecular weight about 18,500, per asymmetric unit.     

Preliminary X-ray investigation of fragment 1 of bovine prothrombin

Crystals of fragment 1 of bovine prothrombin grown from phosphate at pH 7.5 are tetragonal, space group P4₁2₂2 or P4₃2₁2 with $\text{a = b = 79.5}\text{A}\text{?}\text{, c = 84.9}\text{A}\text{?}$, with probably one molecule of 22,000 molecular weight in the asymmetric unit. The presence of 17.5% carbohydrate in the fragment may account for the high liquid content (60%) of the crystals.     

Crystallographic analysis of the phytoagglutinin from Abrus precatorius by X-ray diffraction and electron microscopy

The lectin from the seeds of Abrus precatorius has been crystallized and the crystals subjected to study by X-ray diffraction and electron microscopy. Three closely related crystal forms were obtained, of orthorhombic space group P2₁2₁2₁ with $\text{a = 138}\text{A}\text{?}$, $\text{b = 142}\text{A}\text{?}$, and $\text{c = 173}\text{A}\text{?}$, of tetragonal space group P4₁2₁2 with $\text{a = b = 136}\text{A}\text{?}$, $\text{c = 176}\text{A}\text{?}$, and a twinned intermediate of the first two. From electron microscopy and two-dimensional spatial filtering of electron micrographs of the crystals, the molecule appears to consist of four similar domains grouped in a roughly planar diamond-shaped arrangement having a local intramolecular dyad axis. The average diameter of the Abrus lectin molecule is 50 to 60 Å and the individual domains appear to have a diameter of about 25 Å.     

Preliminary X-ray studies on an unspecific NAD(P)H dehydrogenase from human erythrocytes

Crystals of a relatively unspecific NAD(P)H dehydrogenase from human erythrocytes suitable for X-ray analysis have been grown. They belong to space group P4₁2₁2 or its enantiomorph P4₃2₁2 with unit cell dimensions: $\text{a = b = 79.3}\text{A}\text{?}\text{and}\text{c = 38.1}\text{A}\text{?}$. The asymmetric unit contains one molecule of M_r 18,000.     

Crystallography of alpha-lactalbumin. III. Crystals of baboon milk alpha-lactalbumin.

Crystals of baboon α-lactalbumin suitable for structure analysis at high resolution have been obtained. They have cell dimensions $\text{a = 35.5}\text{A}\text{?}\text{, b = 69.1}\text{A}\text{?}\text{, c = 46.1}\text{A}\text{?}$, space group P2₁2₁2 and contain one molecule of α-lactalbumin per asymmetric unit.     

Crystallization and preliminary crystallographic data for cytochrome c551.5 (c7) from Desulfuromonas acetoxidans.

Cytochrome c₇, a low potential, three heme-containing protein (molecular weight 9800) was isolated from Desulfuromonas acetoxidans and crystallized from polyethylene glycol solutions. The crystals belong to the orthorhombic space group P2₁2₁2₁, with unit-cell dimensions $\text{a = 44·10}\text{A}\text{?}\text{, b = 37·55}\text{A}\text{?}$ and $\text{c = 45·08}\text{A}\text{?}$ and have one molecule of protein per asymmetric unit.     

Preliminary crystallographic data for adenylosuccinate synthetase from rabbit skeletal muscle

Single crystals of adenylosuccinate synthetase, an essential component of purine biosynthesis, extracted from rabbit skeletal muscle were prepared as suitable specimens for X-ray structure analysis. The crystal belongs to the space group P4₃2₁2 or P4₁2₁2 with unit cell dimensions $\text{a = b = 71.2}\text{A}\text{?}\text{, c = 194.8}\text{A}\text{?}$. The asymmetric unit contains one protein molecule of 54,000 molecular weight.     

Letter: Crystallographic data of an L-arabinose-binding protein from Escherichia coli

Single crystals of an l-arabinose-binding protein from Escherichia coli B/r suitable for a high-resolution structural analysis have been obtained. An X-ray examination of the orthorhombic crystals shows the space group is P2₁2₁2₁, with unit cell dimensions: $\text{a = 55.3}\text{A}\text{?}\text{, b = 71.4}\text{A}\text{?}\text{and}\text{c = 77.5}\text{A}\text{?}$. The asymmetric unit contains one protein molecule with a molecular weight of 38,000.     

Crystallizations and preliminary X-ray studies of calotropins DI and DII

Crystals of calotropin DI (M_r 23,400), have been prepared by microdialysis against 5% (w/v) polyethylene glycol 20,000 in water, pH 7.0. They have orthorhombic space group P2₁2₁2₁ with cell parameters $\text{a = 57.5}\text{A}\text{?}\text{, b = 86.2}\text{A}\text{?}\text{, c = 40.3}\text{A}\text{?}$. Crystals of calotropin DII (M_r 24,000), prepared by the same technique against 5% (w/v) polyethylene glycol 20,000 in phosphate buffer of low ionic strength, pH 7.0, display monoclinic space group C2 with cell parameters $\text{a = 135.8}\text{A}\text{?}\text{, b = 32.0}\text{A}\text{?}\text{, c = 47.7}\text{A}\text{?}\text{, β = 103.80 °}$. In both cases, there is only one molecule in the asymmetric unit.     

Letter: Crystallographic study of an esteroproteolytic enzyme

The esteroproteolytic enzyme, a serine protease from porcine pancreas, has been crystallized and characterized by X-ray diffraction. Two closely related crystal forms were observed. The unit cell dimensions of the first form are $\text{a = 59.2}\text{A}\text{?}\text{, b = 96.4}\text{A}\text{?}\text{and}\text{c = 47.4}\text{A}\text{?}$, space group P2₁2₁2 with one molecule (mol. wt 30,000) per asymmetric unit. The unit cell dimensions of the second form are $\text{a = 59.2}\text{A}\text{?}\text{, b = 96.4}\text{A}\text{?}\text{and}\text{c = 94.8}\text{A}\text{?}$, space group P2₁2₁2₁. Mercury derivatives of enzyme inhibitors were used to obtain multiple isomorphous heavy-atom substituents suitable for phase determination.     

Characterization of two crystal forms of neutrophil cationic protein NP2, a naturally occurring broad-spectrum antimicrobial agent from leukocytes

A hexagonal crystal form (P6₃22, $\text{a = b = 34.0}\text{A}\text{?}\text{, c = 113.5}\text{A}\text{?}$) and a monoclinic form (P2₁, $\text{a = 37.1}\text{A}\text{?}\text{, b = 32.2}\text{A}\text{?}\text{, c = 32.4}\text{A}\text{?}\text{, β = 110 °}$) of neutrophil cationic protein NP2, isolated from rabbit leukocytes, have been characterized. The monoclinic form, containing two promoters (M_r = 3844) per asymmetric unit, diffracts to at least 1.8 Å and is suitable for high-resolution structural studies.     

Preliminary X-ray diffraction studies on 2 Fe-ferredoxin from Halobacterium of the Dead Sea

The 2 Fe-ferredoxin from the Halobacterium of the Dead Sea has been crystallized. The space group is P6₃22 with one protein molecule per asymmetric unit. The cell parameters are $\text{a = b = 60.6}\text{A}\text{?}\text{, c = 127.8}\text{A}\text{?}$. The crystals are stable under radiation and diffract to high resolution.     

Structural study of crystalline auromomycin: Preliminary X-ray diffraction study of auromomycin and macromomycin

Auromomycin and macromomycin from the organism Streptomyces macromomyceticus have been crystallized. The X-ray diffraction pattern of crystals of each molecule is consistent with space group P2₁2₁2 with cell parameters $\text{a = 46.45}\text{A}\text{?}\text{, b = 54.34}\text{A}\text{?}\text{and}\text{c = 42.03}\text{A}\text{?}$ for auromomycin, and $\text{a = 46.45}\text{A}\text{?}\text{, b = 54.52}\text{A}\text{?}\text{and}\text{c = 41.54}\text{A}\text{?}$ for macromomycin. Diffraction analysis of auromomycin is in progress.     

Crystal data for tonin, an enzyme involved in the formation of angiotensin II.

Tonin is an enzyme isolated from the submaxillary glands of old rats. This enzyme is involved in the direct conversion of angiotensinogen or the tetradecapeptide renin substrate to the octapeptide angiotensin II, the agent implicated in hypertension. Large well-formed crystals of tonin were grown from 2.5 m-(NH₄)₂SO₄, 0.01 m-phosphate buffer (pH 6.2) and 1% methylpentanediol. The crystals are trigonal, space group P3₁21 (P3₂21) with unit cell dimensions: $\text{a = b = 94}\text{A}\text{?}\text{, c = 66}\text{A}\text{?}\text{, γ = 120°}$. The value of $\text{V}{}_{\mathrm{<mtext>m</mtext>}}\text{= 2.6}\text{A}\text{?}{}^3\text{/}\text{dalton}$ corresponds to one molecule of molecular weight 32,000 per asymmetric unit. Definitive classification of tonin into one of the four proteolytic enzyme classes (carboxyl protease, serine protease, thiol protease or metalloprotease) has not been firmly established.