CHROMOSOME NUMBERS IN COMPOSITAE VIII: HELIANTHEAE

One hundred and ninety-three new counts are reported for the tribe Heliantheae of Compositae, mostly based on determinations of meiotic material, including first counts for the genera Adenothamnus, Chrysogonum, Enceliopsis, Guardiola, Isocarpha, Lipochaeta, Otopappus, and Oyedaea, as well as first counts for 66 species. The original counts are discussed in relation to those previously reported for the tribe, by genera and subtribe. Two-thirds of the approximately 150 genera and more than a third of the roughly 1500 species have now been examined. The incomplete knowledge of generic relationships in the tribe often make the interpretation of these chromosome numbers difficult. Three observations are documented and discussed: (1) genera with low chromosome numbers are few; (2) genera with aneuploid series are abundant; and (3) the original basic chromosome number in the tribe is probably in the range of x = 8 to x = 12.     

CHROMOSOME NUMBERS IN COMPOSITAE V. ASTEREAE II.

Reports of 100 new chromosome counts are made for the tribe Astereae of Compositae, mostly based on determinations of meiotic material, including first counts for 9 genera and 53 species. Counts are now available for 58 of the approximately 100–120 genera and 431 of the approximately 2000 species in the tribe. Comparisons are made between chromosome number and habit and also between chromosome number and geographical distribution. Species and genera with a basic number of x = 9 are the most abundant. Within different phyletic lines x = 9 is also the most abundant number. On the other hand, many species with x = 4 and 5, belonging to a number of small, largely annual genera, are concentrated in southwestern North America. The low chromosome number in these plants is probably correlated with the dry habitat they occupy, and is most likely a specialized condition.     

CHROMOSOME NUMBERS IN COMPOSITAE. IX. HAPLOPAPPUS AND OTHER ASTEREAE

Reports of 209 original chromosome counts are made for the tribe Astereae of Compositae, including first counts for two genera and 46 species or subspecies. With over 80 % of the species counted, chromosome numbers are now available for all North American sections of Haplopappus. Two major groupings are apparent: one, with basic numbers of x = 4, 5, or 6, is basically herbaceous; the other, with x = 9, consists of shrubs or subshrubs. Aneuploidy is known only in the “herbaceous” group of Haplopappus, and polyploidy is more extensive there than in the woodier group of sections.     

Karyomorphological studies in some parasitic species of the Scrophulariaceae,I

A karyomorphological comparison of four genera of the tribe Buchnereae and two genera of the tribe Euphrasieae, root parasites of the subfamily Rhinanthoideae of the Scrophulariaceae, is presented along with 12 new chromosome counts. The interphase nuclei are of the simple chromocenter type. Macranthera and Aureolaria have more subtelocentric chromosomes than those of the other genera studied, which indicates higher asymmetric variation in their karyotypes. In all the karyotypes decrease in size from the longest to the shortest chromosomes is gradual. All the species studied show karyotypic heteromorphology and heteromorphic homologous chromosomes.     

Chromosome numbers in the tribes Anthemideae and Inuleae (Asteraceae)

Twenty-two chromosome counts of 19 taxa (21 populations) in the tribe Anthemideae and one member (one population) of the tribe Inuleae of the family Asteraceae are reported. The Anthemideae studied belong to the subtribes Artemisiinae (14 Artemisia taxa, and one species each of the genera Dendranthema , Filifolium and Neopallasia ) and Tanacetinae (one species each of the genera Lepidolopha and Tanacetopsis ). From the Inuleae, we studied one Inula species. Five counts are new reports (including two at generic level), six are not consistent with previous counts and the remainder are confirmations of very limited (one to four records) previous data. Most of populations of Anthemideae studied have the basic chromosome number x  = 9, with ploidy levels ranging from 2 x to 10 x . Dysploidy is also present, with two x  = 8 diploid taxa. The species of Inuleae studied is a diploid with x  = 10, also indicating dysploidy, other members of the same genus Inula having basic numbers of x  = 9 or 8.  © 2005 The Linnean Society of London, Botanical Journal of the Linnean Society , 2005, 148 , 77–85.     

CHROMOSOME NUMBERS IN THE COMPOSITAE. XIV. LACTUCEAE

Reports of 150 original chromosome counts are recorded, including reports of 22 genera and 57 species and subspecific taxa in tribe Lactuceae. Also included are first reports for 12 specific or subspecific taxa. x = 9 appears to be the ancestral base of the tribe. Chromosome numbers are known for over 85% of the genera of the tribe and the frequency of polyploidy is ca. 23%, which is about one-half that of the angiosperms.     

Cytogenetics and chromosome banding patterns in Caesalpinioideae and Papilionioideae species of Pará, Amazonas, Brazil

Despite the abundance of woody legumes in Brazilian Amazonian rain forests, there are few chromosome counts on the native species of this important region. The present work presents such data for 13 species of Caesalpinioideae (the genera Bauhinia , Caesalpinia , Cassia , Chamaecrista and Senna ) and Papilionioideae ( Bowdichia , Centrosema and Dioclea ) collected from 17 natural populations. Our report represents the first chromosome counts for the genera Bowdichia and Dioclea and for four of the studied species. Observations are made on chromosome morphology, size, condensing behaviour and interphase nucleus structure. Banding with fluorochromes carried out for the first time in Caesalpinioideae revealed discrete CMA⁺/DAPI^– terminal (GC-rich) bands on 2–4 chromosome pairs of most species analysed, with a few species presenting discrete CMA^–/DAPI⁺ (AT-rich) bands. Significant differences in chromosome size, morphology and condensing behaviour were observed among members of the controversial tribe Cassieae ( Cassia , Chamaecrista and Senna ), revealing the tribe to be a heterogeneous group from the karyological point of view.  © 2004 The Linnean Society of London, Botanical Journal of the Linnean Society , 2004, 144 , 181–191.     

Contribution to the karyological knowledge of Echinops (Asteraceae, Cardueae) and related genera

Twenty-six chromosome counts were made of several genera of the tribe Cardueae from various European and Asian provenances: Acantholepis (one species studied), Amphoricarpus (1), Chardinia (1), Echinops (14 species, 15 populations), Siebera (1), Staehelina (3) and Xeranthemum (4). Eleven of the reports are made for the first time, ten confirm previous counts, while the remainder report disparities with earlier records. The existence of different basic chromosome numbers and ploidy levels suggests dysploidy and polyploidy as the main mechanisms of chromosomal evolution in the taxa considered.  © 2004 The Linnean Society of London, Botanical Journal of the Linnean Society , 2004, 145 , 337−344.     

CHROMOSOME NUMBERS IN COMPOSITAE. III. SENECIONEAE

Ornduff , Robert (Duke U., Durham, N. C), Peter H. Raven , Donald W. Kyhos , and A. R. Kruckeberg . Chromosome numbers in Compositae. III. Senecioneae. Amer. Jour. Bot. 50(2): 131–139. Illus. 1963.—Chromosome counts are reported for 75 taxa of tribe Senecioneae (Compcsitae) and are listed with a generic summary of previous counts in the tribe. First counts are reported for Bedfordia, Crocidium, Dimeresia, Gamolepis, Lepidospartum, Luina, Peucephyllum, Telradymia, and the first definite count recorded for Euryops. New numbers are added to those previously known in Arnica and Psathyrotes. Intraspecific differences in ploidy-level are reported in 4 North American species of Senecio. Although chromosome numbers are useful as an aid in delimiting some genera of Senecioneae, they are of little use in circumscribing genera peripheral to Senecio, primarily because of the great range of chromosome numbers of that genus. Chromosome numbers support suggestions based on morphological considerations that genera such as Crocidium and Dimeresia do not belong in Senecioneae, whereas chromosome number and morphology of the plants virtually prohibit the removal of such genera as Peucephyllum, Lepidospartum, and Telradymia from Senecioneae, despite the suggestions of several recent authors. It is proposed that the base number for the tribe is 10 and that the tribe originated in the Old World, with subsequent widespread migration and diversification.     

New or rarely reported chromosome numbers in taxa of subtribe Artemisiinae (Anthemideae, Asteraceae) from Mongolia

This study encompasses 25 chromosome counts of 18 species in the subtribe Artemisiinae (tribe Anthemideae) of the family Asteraceae, from Mongolia. Most (15 species) belong to Artemisia , the largest genus of the subtribe, whereas the others come from two genera very closely related to it: Ajania (two species) and Neopallasia (one species). Eleven counts are new reports, three are not consistent with previous reports and the remainder confirm scanty earlier information. The majority of the species have x  = 9 as their basic chromosome number, but there are some taxa with x  = 8. Ploidy levels range from 2 x to 6 x . The presence of B-chromosomes was detected in Ajania fruticulosa .  © 2006 The Linnean Society of London, Botanical Journal of the Linnean Society , 2006, 150 , 203–210.     

Phylogenetic analyses and chromosome evolution in Convallarieae (Ruscaceae sensu lato), with some taxonomic treatments

The tribe Convallarieae, comprising 10 genera and 95 species, has recently been transferred from its own family to Ruscaceae sensu lato. In this study, sequence data from trnK and rbcL were analyzed for 19 species in 8 genera, and chromosome morphology was analyzed for 17 species in 7 genera. The parsimony analysis of trnK and rbcL sequences showed that Convallarieae are monophyletic. Although early branches did not receive strong bootstrap support, Convallaria diverged at the first branch, followed by Speirantha. The rest of the tribe was split into three, well-supported clades: one with Reineckea, the second with Campylandra and Rohdea, and the third with Tupistra, Tricalistra, and Aspidistra. Two monotypic genera, Rohdea and Tricalistra, were embedded in a clade of Campylandra and of Tupistra, respectively. Three karyotypes were distinguished in the tribe on the basis of the basic number and morphology of metaphase chromosomes: Convallaria type (with x=19 and unimodal chromosome length), Tupistra type (with x=19 and trimodal chromosome length), and Aspidistra-elatior type (with x=18 and trimodal chromosome length). The character-state distribution in the molecular tree showed that the Convallaria type is plesiomorphic, from which was derived the Tupistra type and subsequently the Aspidistra-elatior type. Taxonomic treatments to transfer Campylandra to Rohdea and Tricalistra to Tupistra are also given.     

Molecular Systematics and Evolution of Arabidopsis and Arabis

Abstract: We provide a phylogenetic analysis of the genera Arabidopsis and Arabis based on nuclear ribosomal DNA sequences. We show that traditional taxonomical concepts within tribe Ara-bideae, which includes these genera, are highly artificial. Arabis and Arabidopsis are paraphyletic and consist of several different independent lineages. The genus Capsella, originally placed in tribe Lepideae, is related to North American Arabis and the Arabidopsis thaliana lineage. Other genera, including East Asian Yin-shania, North American Halimolobus, cosmopolitan Barbarea and Cardamine, and European Aubrieta are positioned among different Arabis lineages. One Arabis species, Arabis pauciflora, is only distantly related to tribe Arabideae. Base chromosome number reduction from n = 8 to n = 5 to 7 occurred several times, suggesting that lower base chromosome numbers than n = 8 are derived in tribe Arabideae. Current knowledge on the evolution and systematics of the genera Arabis and Arabidopsis and relationships within the mustard family are summarized and discussed in the light of convergent evolution and transfer of knowledge from Arabidopsis thaliana as a molecular model plant to other species of the Cruciferae.     

CHROMOSOME NUMBERS IN COMPOSITAE. I. ASTEREAE

Raven , Peter H. (U. California, Los Angeles.), Otto T. Solbrig , Donald W. Kyhos , and Richard Snow . Chromosome numbers in Compositae. I. Astereae. Amer. Jour. Bot. 47(2) : 124—132. Illus. 1960.–Ninety-two new counts are reported for the tribe Astereae of Compositae, mostly based on determinations of meiotic material. These include the first counts reported for the genera Acamptopappus, Amphipappus, Benitoa, Chrysothamnus, Corethrogyne, Lessingia, Monoptilon, and Xanthocephalum, as well as for many species. The original counts are discussed in relation to those previously reported for the tribe; together these constitute a total of 39 genera examined cytologically out of the approximately 100 known. Because of its widespread occurrence in diverse phylogenetic lines within the tribe and the family, and because of its high degree of correlation with the woody habit, which is thought to be primitive, x=9 is regarded as the original basic number for Astereae. Within the Haplopappus alliance there is a strong secondary mode of chromosome numbers centering around x=5. The hiatus between these two modes in number is explained on the basis of ancient phylogenetic reduction in chromosome number followed by the extinction of less successful intermediate types, and is compared with similar trends that have been reported for Cichorieae. It is suggested that the family is not of polyploid origin but may have had an original diploid basic number.     

Cladistic analysis of morphological characters in the eulophine tribe Cirrospilini (Hymenoptera: Eulophidae)

A total of 56 morphological characters were analyzed for 53 cirrospiline species that represent all of the 17 described genera of the tribe. The other taxa of the Eulophinae included in the analysis were six species of six representative genera in the tribe Eulophini, a species of Elasmus (the only genus comprising the tribe Elasmini), and a species of Trichospilus (unplaced). Trichospilus and two of the six genera of Eulophini examined were placed within Cirrospilini. Monophyly of Cirrospilini (when these two genera of Eulophini and Trichospilus are included) and of the cirrospiline genera for which more than one species were examined was supported, but the relationships between the genera were poorly resolved. An exception was Cirrospilus, the largest genus in the Cirrospilini, monophyly of which was not supported to any extent.     

CHROMOSOME NUMBERS IN COMPOSITAE. VI. SENECIONEAE. II

Chromosome counts are reported for 76 taxa and 2 natural hybrids of tribe Senecioneae (Compositae). First counts are reported for several species of Senecioneae as well as for the genera Cadiscus and Whitneya. New chromosome numbers are added to those previously known in Arnica, Cacalia, and Senecio. Additional counts from Arnica support our previous suggestion that x = 19 for this genus. It is assumed that observed meiotic irregularities are associated with apomixis in this genus. Basic chromosome numbers for various New World sections of Senecio are proposed, and certain problems of sectional relationships in this genus are discussed. Chromosome numbers and plant morphology of Cadiscus, Hulsea, and Whitneya indicate that these genera should be removed from Helenieae to Senecioneae. The possible affinity of the anomalous genus Adenocaulon with Mutisieae is discussed. Data presented in the paper further support our earlier proposal that the basic chromosome number for Senecioneae is x = 10.     

CHROMOSOME NUMBERS IN COMPOSITAE. II. HELENIEAE

Raven , Peter H. (Rancho Santa Ana Botanic Garden, Claremont, Calif.), and Donald W. Kyhos. Chromosome numbers in Compositae. II. Heleniae. Amer. Jour. Bot. 48(9): 842–850. Illus. 1961.—Chromosome counts are now available for 42 of the approximately 55 genera of Compositae, tribe Helenieae, which is predominantly a group of western North America. These chromosome numbers are summarized here at the generic level, and 100 original counts for the tribe are added, including what seem to be the first published reports for the genera Amblyopappus, Baeriopsis, Hulsea, Jaumea, Pericome, Rigiopappus, Trichoptilium, and Venegasia, as well as for many species. The phylogeny of Chaenactis is discussed in the light of published records and 46 original counts, and C. douglasii is shown to include plants in which n = 6, 12, and 18, which differ somewhat morphologically. Helenium has species which have a complete series of aneuploid numbers from n = 13 to n = 17. Chromosome numbers coincide with morphological variability in indicating that Helenieae are a diverse group. More detailed studies of various kinds will be necessary before the genera of Helenieae can be re-aligned effectively, but it is evident that different genera show affinities with various other tribes of the family. Nevertheless, it is thought to be convenient to continue to recognize Helenieae at the tribal level for the present.     

Physical mapping of rDNA genes in the ground beetle Carabus and related genera (Carabidae: Coleoptera)

The major ribosomal DNA (rDNA) loci were localized on meiotic and mitotic chromosomes and in interphase nuclei of 18 ground-beetle species belonging to three tribes of the supertribe Carabitae by fluorescence in situ hybridization (FISH), using a PCR-amplified 18S rDNA as a probe. Meiotic observations indicate that the 18S rDNA sequences are located on the largest autosomal bivalent in 12 species of Carabus , two species of Calosoma (both genera belonging to the tribe Carabini), and three sibling species of Ceroglossus chilensis (tribe Ceroglossini). The data suggest the occurrence of a conservative pattern in these three genera despite the chromosomal rearrangements that have led to karyotypes with higher chromosome numbers in Ceroglossus . A different result is found in Cychrus caraboides (tribe Cychrini), where ribosomal cistrons are located in two medium-sized autosomal pairs. Further species of Cychrini should be studied for corroborating the occurrence of molecular and karyotypical apomorphies in Cychrus with regard to the genera Carabus, Calosoma and Ceroglossus .     

栌菊木属及白菊木属的细胞学研究

对栌菊木属及白菊木属的染色体数目进行了首次报道,对栌菊木（Nouelia insignis Franch.)分布区内的10个居群进行了细胞学研究,染色体数目均为2n=54。白菊木属在中国分布的仅白菊木（Leucomeris decora Kurz)一种,对漾濞这个居群的细胞学研究表明,染色体也为2n=54。这两个属的基数可能x=9,它们可能为6倍体,结合帚木菊族已有染色体报道及形态特征,地理分布等初步分析表明：栌菊木种内分化程度小,是一自然类群;栌菊木和白菊木可能有着较近缘的联系;栌菊木可能是古老孑遗植物的后裔,为适应环境而多倍体化,得以保存下来。     

New chromosome counts and evidence of polyploidy in Haageocereus and related genera in tribe Trichocereeae and other tribes of Cactaceae

Chromosome numbers for a total of 54 individuals representing 13 genera and 40 species of Cactaceae, mostly in tribe Trichocereeae, are reported. Five additional taxa examined belong to subfamily Opuntioideae and other tribes of Cactoideae (Browningieae, Pachycereeae, Notocacteae, and Cereeae). Among Trichocereeae, counts for 35 taxa in eight genera are reported, with half of these (17 species) for the genus Haageocereus. These are the first chromosome numbers reported for 36 of the 40 taxa examined, as well as the first counts for the genus Haageocereus. Both diploid and polyploid counts were obtained. Twenty nine species were diploid with 2n=2x=22. Polyploid counts were obtained from the genera Espostoa, Cleistocactus, Haageocereus, and Weberbauerocereus; we detected one triploid (2n=3x=33), nine tetraploids (2n=4x=44), one hexaploid (2n=6x=66), and three octoploids (2n=8x=88). In two cases, different counts were recorded for different individuals of the same species (Espostoa lanata, with 2n=22, 44, and 66; and Weberbauerocereus rauhii, with 2n=44 and 88). These are the first reported polyploid counts for Haageocereus, Cleistocactus, and Espostoa. Our counts support the hypothesis that polyploidy and hybridization have played prominent roles in the evolution of Haageocereus, Weberbauerocereus, and other Trichocereeae.     

Revision of generic limits in Hyptis Jacq. (Labiatae) and its allies

The primarily neotropical sub-tribe Hyptidinae (Labiatae: tribe Ocimeae) consists of six or seven genera of which the largest is the genus Hyptis with over 300 species. Over the last 12 years, work on the group at the Royal Botanic Gardens, Kew, examining macro-morphological and anatomical characters, as well as pollen surface and chromosome number, has indicated that the genus Hyptis is paraphyletic, with a few of the larger-flowered sections apparently being more closely related to the neighbouring genus Eriope than to the smaller-flowered sections of Hyptis . This has been confirmed by preliminary cladistic studies now in progress. In order to provide a working classification for the group, prior to the publication of the more detailed study, two new genera: Hypenia and Hyptidendron are created. A key to genera of South American Hyptidinae is provided, as well as one to distinguish the two new sections of Hyptidendron . Forty-five new combinations are made.