首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 31 毫秒
1.
Bisalputra, T., and T. E. Weier. (U. California, Davis.) The cell wall of Scenedesmus quadricauda. Amer. Jour. Bot. 50(10): 1011–1019. Illus. 1963.—Fine structure of the cell wall of Scenedesmus quadricauda fixed in both KMnO4 and osmium tetroxide is described. The cell wall consists of 3 layers: the inner cellulosic layer which delimits individual cells; the outer pectic layer which binds the cells of the coenobium together; and a thin middle layer, bounded by membranes on either side, which is electron-dense in osmium-fixed material but of medium electron density in KMnO4. The structure of the outer pectic layer is similar in both fixatives; it consists of a hexagonal network of electron-dense material on the surface, and a system of tubules or “props” which radiate out from the middle layer of the wall to support the net. The pectic layer appears in the daughter coenobia before their liberation from the parent colony.  相似文献   

2.
Dawes , Clinton J., and Edwin Bowler . (U. of California, Los Angeles.) Light and electron microscope studies of the cell wall structure of the root hairs of Raphanus sativus. Amer. Jour. Bot. 46(8): 561–565. Illus. 1959.—The structure and development of the cell wall of the root hair of Raphanus sativus were studied under the light and electron microscopes. The outer layer of the root hair consists of mucilage which covers the entire hair and forms a thick cap at the tip. Beneath the mucilage a thin cuticle covers the inner layers of the cell wall. These layers consist of cellulose microfibrils, varying in pattern, in a granular matrix, presumably pectic in nature. The microfibrils of the outer layer, apparently laid down at the tip, are reticulate in arrangement. In mature regions of the root hair, the wall is thickened by an inner layer of parallel and longitudinally orientated microfibrils. Pores in the cellulose wall are evident and increase in number and size near the base of the hair.  相似文献   

3.
Study of the pear leaf cuticle (Pyrus communis L. ‘Bartlett‘), in both intact and enzymatically isolated forms, has revealed that the cuticular membrane is separated from the underlying epidermal cell wall by a layer of pectic substances which extend into but not through the membrane. A layer of embedded birefringent waxes occurs towards the outer surface of the cuticular membrane. Platelet-like epicuticular waxes are deposited on the outer surface. The upper cuticular membrane is astomatous. The lower epidermis is stomatous, and the outer cuticular membrane is continuous with that lining the substomatal cavity. The lower cuticular membrane is also generally thicker than the upper, and both the upper and lower cuticular membranes are thicker over veinal than over mesophyll tissue. The birefringence frequently is discontinuous over anticlinal walls and over veinal tissue. The lower cuticle appears to contain fewer embedded waxes (as indexed by birefringence) than the upper. Enzymatic isolation of the cuticular membrane from the underlying tissues does not appear to cause any discernible change in structure as viewed with a light microscope. These findings are discussed in light of current knowledge concerning penetration of foliar applied substances into the leaf.  相似文献   

4.
In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr. Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole. Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading. The first visual change in the cells of the abscission zone was a swelling of the pectic materials of the cell walls. This was followed by breakdown of other cell wall components, i.e., non-cellulosic polysaccharides and cellulose. The cellulose of the cell walls adjacent and distal to the abscission layer was found to be altered; however, no lignin was present during abscission layer development. The alteration of pectic materials, coupled with breakdown of cell wall components, resulted in the collapse of cells of the abscission layer just prior to separation. Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight.  相似文献   

5.
The ultrastructure of the wall layers and ornamentative features of Scenedesmus pannonicus and S. longus are described using carefully correlated freeze-etched replicas, thin sections and scanning electron micrographs. The two species arc enclosed by different types of ornamented layers, S. pannonicus by the tightly filling, “warty” layer and S. longus by the loosely fitting, “reticulate” layer, held off the coenobium by 2 types of tubular propping spikelets and rosettes. The reticulate layer has an intricate substructure, especially when studied with freeze-etching. Its inner and outer surfaces appear different, as is its attachment to the 2 types of spikelets. Whole cells of S. longus subjected to acetolysis lack the cellulose wall and cytoplasm, but all other surface features survive, including the Trilaminar Sheath (TLS); this ornamentation cannot be “pectic.” The cellulose wall and ornamentation is unaffected by boiling water alone. Boiling in 6n NaOH removes the surface ornamentation, but the TLS and wall remain; the possibility that these features contain silica is discussed. The terminal spines of both species consist of closely packed spikelets enclosed within a skin of hexagonally-packed subunits. Similar subunits are seen in the propping spikelets of S. longus, and in the rows or “combs” of laterally fused spikelets of S. pannonicus. The warty layer of S. pannonicus is tightly appressed to the TLS except close to where the cells are joined, where it is suspended free. It is composed of a layer of globular subunits, and small indentations form the warts. Single, evenly distributed warts characterize the freely suspended sections of the warty layer, and the layer that encloses young coenobia soon after they have been formed: in contrast, the warts are clumped over the surface of older and larger colonies. Some of the single warts form characteristic double rows, but these latter remain single even on older cells. The surface structure of the warty layer, TLS, and plasmalemma are revealed by the freeze-etching process.  相似文献   

6.
Abscission was studied in Psoralea argophylla Pursh, a prairie tumbleweed. This process is responsible for the detachment of the stem from its perennial base. In a single plant abscission may proceed through one or more intercalary meristems located at or near ground level. During senescence a separation layer differentiates within each of these meristems. Chemical changes occur within the compound middle lamellae which result in the conversion of insoluble pectic compounds into soluble forms. Cell separation takes place only after removal of the pectic compounds, meristematic activity, and attenuation of cell walls. Unlike reports of other angiosperms the protective layer is not formed adjacent to the separation layer, but forms some distance underground at the stem-root junction.  相似文献   

7.
The spermatozoids of Zamia integrifolia were examined by electron microscopy in order to clarify the structure of a fibrous organelle underlying the flagellar basal bodies. The ultrastructure of this organelle was found to resemble one similar in organization occurring in Marchantia spermatozoids and termed the Vierergruppe. Therefore the term is extended to include this structure in the Zamia spermatozoid. The Vierergruppe of Zamia is interpreted as composed of an upper (distal) layer of keeled microtubules and a lower (proximal) layer of vertical fins with lateral processes that give a three-layered appearance to this component.  相似文献   

8.
The ultrastructure, cuticle, and distribution of pectic epitopes in outer periclinal walls of protodermal cells of Daucus carota zygotic and somatic embryos from solid and suspension culture were investigated. Lipid substances were present as a continuous layer in zygotic and somatic embryos cultured on solid medium. Somatic embryos from suspension cultures were devoid of cuticle. The ultrastructure of the outer walls of protodermis of embryos was similar in zygotic and somatic embryos from solid culture. Fibrillar material was observed on the surface of somatic embryos. In zygotic embryos, in cotyledons and root pectic epitopes recognised by the antibody JIM5 were observed in all cell walls. In hypocotyls of these embryos, these pectic epitopes were not present in the outer periclinal and anticlinal walls of the protodermis. In somatic embryos from solid media, distribution of pectic epitopes recognised by JIM5 was similar to that described for their zygotic counterparts. In somatic embryos from suspension culture, pectic epitopes recognised by JIM5 were detected in all cell walls. In the cotyledons and hypocotyls, a punctate signal was observed on the outside of the protodermis. Pectic epitopes recognised by JIM7 were present in all cell walls independent of embryo organs. In zygotic embryos, this signal was punctate; in somatic embryos from both cultures, this signal was uniformly distributed. In embryos from suspension cultures, a punctate signal was detected outside the surface of cotyledon and hypocotyl. These data are discussed in light of current models for embryogenesis and the influence of culture conditions on cell wall structure.  相似文献   

9.
The sheath of Volvox possesses a surface layer which hits not previously been described. The application of new staining techniques enhances its visualization.  相似文献   

10.
Carlquist , Sherwin . (Rancho Santa Ana Botanic Garden, Claremont, Calif.) The leaf of Calycadenia and its glandular appendages. Amer. Jour. Bot. 46(2) : 70-80. Illus. 1959.—Large tack-shaped glands are characteristic of the leaves of Calycadenia which are associated with the inflorescence. These glands may be divided into those which are terminal on leaves and those which occur laterally on the surface of the leaf. Lateral glands show stages early in their development which are identical with those of simpler trichomes of Madinae. Terminal glands, which possess more vascularization of the stalk, show a more modified form of development. Vascularization is not derived from protoderm, but from more deeply-seated cells. These cells are included in a zone of elongation which forms the stalk. Vascular bundles may extend to the base of glands which lack vascularization in their stalks. Tack-shaped glands are considered an advanced form of trichome in which internal tissues of the leaf are involved. Within the genus Calycadenia, ontogenetic and comparative studies suggest that the following characters are advanced: reduction to a single terminal gland, “inrolling” of margins to form a cylinder of bundles, concomitant with a central core of fibers or a pectic channel. Systematic distribution of gland occurrence and of types of foliar structure are given.  相似文献   

11.
All Rhagoletis reportedly establish associations with one or more bacterial species, but the bases for these interactions and their implications for host race formation and speciation are poorly understood. Here we present the results of four studies designed to increase our understanding of these relationships. In the first study, we identify the bacteria associated with seven Rhagoletis taxa by surveying the inhabitants of the esophageal bulb, an organ whose major function appears to be the housing of microorganisms. The results suggest that no bacterium has entered into an obligate symbiotic relationship with any of the Rhagoletis taxa surveyed, although one bacterium, Klebsiella oxytoca, is a very common associate of six of the seven. In the second study we use horizontal starch gel electrophoresis to determine the genetic similarity of K. oxytoca clones isolated from different Rhagoletis populations. This analysis provides a rare look into the genetic structure of natural populations of an enteric bacterium and permits the construction of a dendrogram for the clones—a dendrogram which indicates that there is no clear-cut pattern to the distribution of K. oxytoca genotypes among Rhagoletis. Taken together, the above studies provide indirect evidence that the bacteria associated with Rhagoletis are not important determinants of host plant specificity. The third and fourth studies assess two possible functions associated bacteria may perform for Rhagoletis: pectic substances degradation and nitrogen fixation. Our results do not lend support to either function.  相似文献   

12.
The pollen morphology of eight species of Nigella (Ranunculaceae) was examined by scanning and transmission electron microscopy. The exomorphology of all species was identical: 3-colpate, spinulose, and punctate, but thin sections revealed two structural patterns. The ektexine structure of Nigella integrifolia, consisting of thickened foot layer, columellae, and thin tectum, is typical for the family as well as the order Ranunculales in general. In contrast, the remaining seven species, N. arvensis, N. damascena, N. elata, N. hispanica, N. sativa, N. segetalis, and N. stellaris, have an ektexine with an additional unit, a horizontal layer with shorter columellae, placed between the foot layer and tectum. Of all genera examined in the Ranunculaceae, only Nigella had this unusual stratification. This difference in the exine structure would add support to the treatment of N. integrifolia as a monotypic genus, Komaroffia integrifolia (Regel) Lemos Pereira.  相似文献   

13.
Multinucleate cells of Coelastrum undergo precisely directed cytokinesis, guided by phycoplast microtubules, to form a number of uninucleate daughter cells which subsequently adhere to form characteristically patterned aggregates. As there is no movement of the daughter cells relative to one another before their adhesion, the disposition of cells in daughter colonies reflects the pattern of cytokinesis of parent cells. Centrioles lie at the poles of the mitotic nuclei which are partially enclosed by a perinuclear envelope of endoplasmic reticulum. The centrioles disappear at the time of cytokinesis of the parental cell and apparently reform de novo once the daughter cells have acquired a cell wall following their adhesion. The trilaminar layer of cell wall, often termed the pectic layer, does not stain with ruthenium red and resists acetolysis suggesting that it contains sporopollenin rather than pectin.  相似文献   

14.
Pectin: cell biology and prospects for functional analysis   总被引:27,自引:0,他引:27  
Pectin is a major component of primary cell walls of all land plants and encompasses a range of galacturonic acid-rich polysaccharides. Three major pectic polysaccharides (homogalacturonan, rhamnogalacturonan-I and rhamnogalacturonan-II) are thought to occur in all primary cell walls. This review surveys what is known about the structure and function of these pectin domains. The high degree of structural complexity and heterogeneity of the pectic matrix is produced both during biosynthesis in the endomembrane system and as a result of the action of an array of wall-based pectin-modifying enzymes. Recent developments in analytical techniques and in the generation of anti-pectin probes have begun to place the structural complexity of pectin in cell biological and developmental contexts. The in muro de-methyl-esterification of homogalacturonan by pectin methyl esterases is emerging as a key process for the local modulation of matrix properties. Rhamnogalacturonan-I comprises a highly diverse population of spatially and developmentally regulated polymers, whereas rhamnogalacturonan-II appears to be a highly conserved and stable pectic domain. Current knowledge of biosynthetic enzymes, plant and microbial pectinases and the interactions of pectin with other cell wall components and the impact of molecular genetic approaches are reviewed in terms of the functional analysis of pectic polysaccharides in plant growth and development.  相似文献   

15.
Abstract: The spore Rhabdosporites (Triletes) langii (Eisenack) Richardson, 1960 is abundant and well preserved in Middle Devonian (Eifelian) ‘Middle Old Red Sandstone’ deposits from the Orcadian Basin, Scotland. Here it occurs as dispersed individual spores and in situ in isolated sporangia. This paper reports on a detailed light microscope (LM), scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis of both dispersed and in situ spores. The dispersed spores are pseudosaccate with a thick walled inner body enclosed within an outer layer that was originally attached only over the proximal face. The inner body has lamellate/laminate ultrastructure consisting of fine lamellae that are continuous around the spore and parallel stacked. Towards the outer part of the inner body these group to form thicker laminate structures that are also continuous and parallel stacked. The outer layer has spongy ultrastructure. In situ spores preserved in the isolated sporangia are identical to the dispersed forms in terms of morphology, gross structure and wall ultrastructure. The sporangium wall is two‐layered. A thick coalified outer layer is cellular and represents the main sporangium wall. This layer is readily lost if oxidation is applied during processing. A thin inner layer is interpreted as a peritapetal membrane. This layer survives oxidation as a tightly adherent membranous covering of the spore mass. Ultrastructurally it consists of three layers, with the innermost layer composed of material similar to that comprising the outer layer of the spores. Based on the new LM, SEM and TEM information, consideration is given to spore wall formation. The inner body of the spores is interpreted as developing by centripetal accumulation of lamellae at the plasma membrane. The outer layer is interpreted as forming by accretion of sporopollenin units derived from a tapetum. The inner layer of the sporangium wall is considered to represent a peritapetal membrane formed from the remnants of this tapetum. The spore R. langii derives from aneurophytalean progymnosperms. In light of the new evidence on spore/sporangium characters, and hypotheses of spore wall development based on interpretation of these, the evolutionary relationships of the progymnosperms are considered in terms of their origins and relationship to the seed plants. It is concluded that there is a smooth evolutionary transition between Apiculiretusispora‐type spores of certain basal euphyllophytes, Rhabdosporites‐type spores of aneurophytalean progymnosperms and Geminospora‐/Contagisporites‐type spores of heterosporous archaeopteridalean progymnosperms. Prepollen of basal seed plants (hydrasperman, medullosan and callistophytalean pteridosperms) are easily derived from the spores of either homosporous or heterosporous progymnosperms. The proposed evolutionary transition was sequential with increasing complexity of the spore/pollen wall probably reflecting increasing sophistication of reproductive strategy. The pollen wall of crown group seed plants appears to incorporate a completely new developmental mechanism: tectum and infratectum initiation within a glycocalyx‐like Microspore Surface Coat. It is unclear when this feature evolved, but it appears likely that it was not present in the most basal stem group seed plants.  相似文献   

16.
The seed coats of Gnetum gnemon L., G. ula Brongn., G. montanum f. parvifolium (Warb.) Mgf. and G. neglectum Bl. consist of three layers. The outer layer or sarcotesta is mostly parenchyma but contains some sclereids and fibers and a series of simple vascular bundles. The middle sclerotesta forms masses of sclereids in varying shapes and numbers, sometimes extending as a basal plate, and is usually thicker near the micropylar tube. The second layer also contains a series of small vascular bundles that reach the apex. Depending on the species, the middle layer is sometimes nearly free from the outer layer, may be partially fused with it, or completely fused to it at maturity. The innermost layer of the seed coat constitutes the endotesta which is membranous and only rarely contains sclerenchyma. It possesses a dichotomous venation system with varying degrees of anastomosing, depending upon the species. The above species show qualitative and quantitative differences in their sclerenchyma and laticifers. Seed coat anatomy may be useful in the diagnosis of some species. The trends of evolution of seed coat structure within these four species of Gnetum are discussed, and a comparison of tissue layers and vasculature with certain fossil pteridosperms is made, especially in the Trigonocarpales  相似文献   

17.
The structure of the Royal Ann cherry cuticle (Prunus avium L.) was determined and interpreted in the light of its possible significance to cuticular penetration by a SO2-calcium bisulfite brine. The morphology of the cuticle was determined by standard histological and histochemical techniques. The surface structure of the cuticle was found to have a smooth to granular sheet or layer of surface wax, which when removed revealed a porous sponge-like surface. The cuticular surface showed intermittent birefringence, which increased as the fruit matured. Ectodesmata were found to occur over anticlinal walls and in guard cells on both sides of the fruit, with more on the side opposite the suture. Both sides had stomata with more occurring on the suture side. Secondary bleaching was found to alter the structure and permeability of the cuticle.  相似文献   

18.
STAINED PECTIN AS SEEN IN THE ELECTRON MICROSCOPE   总被引:4,自引:0,他引:4       下载免费PDF全文
This paper describes electron microscopic studies on the distribution of pectin within young plant cells. Dark-grown onion roots, from 1 to 3 mm. in length, were used. In order to make the pectic substances selectively dense to electrons, they were first reacted with basic hydroxylamine. This treatment produces pectic hydroxamic acids, which in turn were treated with ferric ion to form insoluble complexes. The tissue was imbedded, sectioned, and then observed by electron microscopy. Dense deposits of iron were found in the region of the middle lamella and in a second area near the surface of the primary wall. Transverse walls of varying maturity were noted. The pectin of the more frequent, immature cross-walls, leads directly into the inner reacting layer of the axillary (longitudinal) wall. The pectin of the more mature transverse walls becomes, on the other hand, intimately associated with the middle lamella pectin of the axillary wall. It is shown that the pectin of the middle lamella represents the hot water-soluble portion of the pectic substance, while the internal layer of the axillary wall and the transverse wall pectin represent the so called residual fraction. Hot versene extraction removes essentially all electron-dense material.  相似文献   

19.
The pseudocapillitium and spores of L. epidendrum were studied by transmission (TEM) and scanning electron microscopy (SEM). The SEM reveals that the pseudocapillitial surface is covered by bands of “wartlike” processes that alternate with non-ornamented regions. Otherwise, the pseudocapillitium is a hollow structure composed of three regions. The outer region is thin, electron dense and continuous with many irregular processes. Internal to this area is an amorphous region containing scattered electron dense material. The innermost region of the pseudocapillitium is thin, inconspicuous and usually electron dense. L. epidendrum possesses spores that are covered by a surface reticulum consisting of polygonal areas which are continuous with the outermost spore layer. The outer spore layer is thin and electron dense. The inner spore layer is an electron transparent region that contains granular or fibrillar components. Sections of spores showed a dense cytoplasm possessing most of the usual organelles along with microtubules and microbodies.  相似文献   

20.
An isolate of Verticillium dahliae obtained from Uganda was highly virulent to young cotton plants under greenhouse conditions. A hyaline variant which often appeared in culture was as virulent as the parent isolate, but preliminary experiments indicated that it did not survive as long in unsterile soil. The parent isolate grew rapidly in cotton plants after root inoculation and was isolated from stems and leaves well before the appearance of disease symptoms visible to the naked eye.
Protopectinase was produced in the absence of pectic materials, but more active preparations were obtained when media contained pectic substances. In general, there was a close correspondence between the protopectinase activity of culture filtrates and the toxicity of these filtrates to parenchymatous cells. Some separation of the two activities was obtained by heating enzyme solutions or by plasmolysing the test tissue.
Protopectinase solutions had little pectinesterase activity but rapidly reduced the viscosity of solutions of pectic substances. In general, the properties of protopectinase and the viscosity-reducing enzyme were similar.
Young cotton shoots wilted rapidly when placed in cell-free filtrates from cultures of the pathogen. Wilting was delayed under conditions unfavourable for transpiration. Evidence was obtained which showed that wilting was caused by the uptake of thermostable compounds of high molecular weight which impeded the upward flow of the vascular sap. Pronounced vascular browning was obtained only when solutions containing protopectinase were used. Wilting and vascular browning were obtained with solutions having little pectinesterase activity; in contrast, a solution having high pectinesterase activity produced relatively little vascular browning.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号