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1.
Diploid (2n = 20) and tetraploid (2n = 40) Zea mays L. were crossed with diploid (2n = 36) and tetraploid (2n = 72) Tripsacum dactyloides (L.) L. to produce a series of hybrids combining different numbers of haploid genomes from each parent. Eight hybrid groups and three parental groups were studied morphologically. Twenty-nine quantitative characters were recorded for each sample. Data were analyzed by univariate analysis of variance, multivariate analysis of variance, and discriminant function analysis, in an attempt to evaluate hybrid differences objectively and determine which morphological characters contribute statistically to group separation. The overall MANOVA F test was significant, establishing the presence of real differences between the hybrids; discriminant function analysis indicated that the percent of paired pistillate spikelets/cupule in the lateral inflorescence was the main variable which differentiated hybrids. Duncan's Multiple Range Tests for significant differences between means were applied to five variables contributing maximally to group discrimination, using the appropriate univariate ANOVAs. Pronounced maize-like attributes of backcross hybrids, as compared with corresponding F1's possessing similar genome constitutions, gave possible evidence of gene transfer between Zea mays and Tripsacum during backcrossing to maize. 相似文献
2.
Derivatives of a cross between diploid Zea mays L. and Tripsacum dactyloides (L.) L. (2n = 72) were compared cytologically and morphologically. The objective of this study was to detect introgression from Tripsacum to maize that might have occurred during seven backcross generations with maize. Thirty-three morphological characters were used to analyze variation among aneuploid (20Zm + 2Td), 20-chromosome recovered maize, and the recurrent maize parent plants. Aneuploid and maize checks were extreme types, with 20-chromosome hybrid derivatives being morphologically intermediate. Several recovered maizes clustered with aneuploid plants and these hybrid derivatives have the greatest chance of Tripsacum introgression. Many traits such as endosperm abnormalities, tassel seed, albinos, tunicate glumes, tassel-tipped ears, fasciated and branched ear, and male spikelets between rows of kernels were observed. Although the genetic basis of many traits is unknown, mutations, epistatic effects or expression of Tripsacum chromatin are possible causes. The number of abnormal and tripsacoid traits observed in 20-chromosome recovered maizes indicates genetic transfer from Tripsacum to the maize genome. 相似文献
3.
The genus Glycine Willd. is divided into three subgenera, Glycine Willd., Soja (Moench) F. J. Herm., and Bracteata Verdc. Six species are currently recognized in the subgenus Glycine: G. canescens F. J. Herm., G. clandestina Willd., G. falcata Benth., G. latrobeana (Meissn.) Benth., G. tabacina (Labill.) Benth., and G. tomentella Hayata. Distribution of the subgenus extends from south China to Tasmania and includes several Pacific islands. A collection of these species was examined cytologically and morphologically in an attempt to evaluate existing variability between and within taxa. Chromosome counts confirmed G. canescens, G. clandestina and G. falcata to be diploid with 2n = 40. Both tetraploids (2n = 80) and diploids were found in G. tabacina, the latter restricted to Australia. Glycine tomentella accessions were primarily tetraploid, but several collections from New South Wales, Australia, were found to be aneuploid with 78 chromosomes. One collection was aneuploid at the diploid level with 38 chromosomes. Meiosis appeared normal in the aneuploids with regular bivalent formation. Several accessions previously identified as G. tomentella were diploid. Seed of G. latrobeana was not available for analysis. Numerical techniques in the form of cluster analysis and principal components analysis were applied to morphological data on vegetative and inflorescence characters obtained from each collection. Numerical analysis grouped the accessions essentially according to current species delimitations with some exceptions. Glycine tabacina specimens from Taiwan approached G. clandestina in several characteristics. The diploid G. tomentella specimens formed a separate cluster and appeared morphologically distinct from the remaining taxa. 相似文献
4.
Intermatings between sexual diploids (2n=36) and facultative apomictic tetraploids (2n=4n=72) of Tripsacum dactyloides var. dactyloides, (L.), L., have been generated. Subsequent exchange and addition of genetic material between the two cytotypes, via a backcrossing program, provides one method for the manipulation of genetic elements involved in apomictic reproduction (diplospory) of this species.Identification and development of fertile triploids, and their innate tendency to exhibit a high frequency of unreduced egg cells which remain receptive to fertilization by a sperm nucleus, allows for the unique development of B
III
(2n=3x+n) tetraploids by genome accumulation. The resultant B
III
progeny provide new breeding stock for investigating the inheritance and generation of such materials also allows for the occurrence of genetic recombination between sexual diploids and apomictic tetraploids. Potential use of fertile triploids, their tendency for genome accumulation and their use in exploiting apomictic tetraploid germplasm resources are also discussed.All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. 相似文献
5.
H. Hartings N. Lazzaroni V. Rossi M. Motto 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(6):696-701
Thirty-four accessions from Zea and 10 accessions from related genera were assayed for the presence of Bg, a transposable element originally found in maize (Zea mays ssp. mays). Bg-like sequences, identified as hybridizing bands on Southern blots, were visualized in all Zea accessions and were present in approximately equal numbers in teosinte and maize. With the exception of Tripsacum dactyloides, all accessions from related genera failed to hybridize with the Bg probes, even at reduced stringency. A comparison of the restriction patterns of related inbred lines revealed numerous common hybridizing fragments. An index of molecular similarity (MS) was used to determine the degree of similarity between pairs of inbred lines. Computed MS values endorse an inbred relationship and are in good agreement with published results of cluster analysis on these inbred lines. 相似文献
6.
Heuchera micrantha (Saxifragaceae) is a morphologically variable species comprising five varieties: diversifolia, erubescens, hartwegii, micrantha, and pacifica. Both diploids (2n = 14) and tetraploids (2n = 28) occur within the species. The tetraploid cytotype occupies the central portion of the geographic range of the species, whereas diploids occur primarily in the southern and northern portions of the range. Both diploids and tetraploids have been detected within vars. diversifolia, pacifica, and hartwegii. All counts for vars. erubescens and micrantha are diploid and tetraploid, respectively. Several lines of evidence suggest that tetraploid H. micrantha is of autopolyploid origin. The species is distinct morphologically and is also well separated geographically from other closely related species in subsection Micranthae. The two cytotypes are karyologically identical, possess nearly the same suite of allozymes, and have a very high genetic identity (Ī = 0.971). Significantly, an earlier study documented tetrasomic inheritance in the tetraploid cytotype. Following theoretical expectations, the mean number of alleles per locus, proportion of loci that are polymorphic, and observed heterozygosity are significantly higher for the autotetraploid than for the diploid. The occurrence of both cytotypes in three of the varieties suggests that autopolyploidy may have occurred several times independently in H. micrantha. This was further substantiated by discriminant analysis using morphological characters, which provided evidence for a minimum of two separate origins for the autopolyploid cytotype. 相似文献
7.
E. Falistocco 《Plant biosystems》2016,150(4):692-701
Dactylis glomerata L. is one of the best known examples of a polyploid complex that includes diploid (2n = 14), tetraploid (2n = 28) and hexaploid (2n = 42) types. The diploids, descending from the ancestors of the genus, are numerous, distinct forms adapted to specific environments and include all the morphological variation and ecological adaptation found in Dactylis. The objective of the present study was to shed more light on the differentiation of diploids and to obtain information for better exploiting the genetic resources of this species. To this end we carried out a discriminant analysis in the most widespread diploid subspecies based on a large number of morpho-physiological traits. We found that some characteristics, such as time to heading, time to flowering, vegetative regrowth, plant height and seed shattering are the most discriminant and enabled us to realize a more exact differentiation of the diploids with respect to the morphological criterion generally adopted. The results demonstrated that diploid taxa offer an interesting combination of relevant agronomical and ecological characteristics which could be useful in breeding programs to promote the use of this species in sustainable agriculture systems. We also performed cytological investigations and defined a general karyotype representative of the diploid Dactylis considered in this study. 相似文献
8.
Eastern gamagrass, (Tripsacum dactyloides L.) is a perennial, warm-season grass that is being developed as a forage plant. Shoots were derived from callus initiated
from immature embryos and immature inflorescences of diploid (2n=2x=36) gynomonoecious eastern gamagrass. These shoots were
induced to microtiller in the presence of 3 mg/l benzyladenine. Amiprophosmethyl (10, 15, or 20 μm) was applied to 27 microtillers for 3–5 days to induce chromosome doubling. All 14 surviving plants were tetraploid, (2n=4x=72),
as determined by flow cytometry or chromosome counts. These plants were morphologically normal and produced seed. Test crosses
were made with a known diploid. Flow cytometry and chromosome counts showed that the progeny were triploid, proving that the
induced tetraploids reproduce sexually.
Received: 12 February 1997 / Revision received: 18 February 1998 / Accepted: 13 March 1998 相似文献
9.
Black nightshades are a group of species best known for their ‘poisonous’ or noxious weedy reputation. It is not so well known
that species of this group serve as emerging food source in many countries worldwide especially in the African continent.
Despite the fact that the section has recently been studied extensively, taxonomy is still unsettled and debated because of
inter- and intraspecific hybridization, phenotypic plasticity and polyploidization. In this study we analyze the genetic relationships
among diploid, tetraploid and hexaploid species of sect. Solanum, which have possibly taken part in the formation of Solanum nigrum, utilizing multi-locus (SCoT, ISSR) markers combined with chloroplast trnL-F sequence data and morphological characters. We scored 51 morphological characters united with SCoT (171), ISSR (224) and
trnL-F (1042), for simultaneous analysis of 49 terminals and 1488 characters. The topology of the tree is concordant with the results
of the network analysis. In the phylogenetic networks, all the accessions of the diploid species shared a split with all of
the polyploid species. This reflected a high portion of shared ISSR and SCoT bands between diploids and polyploids. In addition,
a strong split divided the diploid species. The history of S. nigrum might be reticulate with hybrid speciation playing an important rule. Genetically differentiated diploids in few combinations
have created a series of genetically distinct polyploid populations. The insufficient isolation that permitted further recombination
between ancient polyploids and diploids have resulted in high level of genotypic and phenotypic polymorphism. This high level
of novel genomic variability obviously enabled species to succeed in their new environment. 相似文献
10.
In order to study how polyploidy affects life history patterns in animals, we have examined sympatric diploid and polyploid brine shrimp (Artemia parthenogenetica) from China, Italy and Spain under laboratory conditions. At optimal temperature and salinity (25°C and 90 ppt), diploids from the three populations had much higher intrinsic rates of increase, higher fecundity, faster developmental rates, and larger brood sizes than their sympatric polyploids. The Chinese and Italian populations were selected for further analysis to determine the life history responses of diploids and polyploids to temperature and salinity changes. Under intermediate and high salinities, Chinese and Italian polyploids produced most of their offspring as dormant cysts while their sympatric diploids produced most of their offspring as nauplii. This relationship is reversed in the Spanish diploid-polyploid complex. For the Chinese population at 25° C, pentaploid clones had higher developmental rates than diploid clones at 35 ppt; at 90 ppt, diploid clones had higher developmental rates than the pentaploids. Italian diploids and tetraploids had different responses to variation in both temperature (25° C and 31° C) and salinity (30 ppt and 180 ppt). Our results demonstrate that relative fitness of the two cytotypes is a function of environmental conditions and that sympatric diploids and polyploids respond differently to environmental changes. Chinese and Italian polyploids are expected to have lower fitness than their sympatric diploids when the physical environment is not stressful and when intraspecific competition is important. However, polyploids may have advantages over sympatric diploids in stressful habitats or when they encounter short-term lethal temperatures. These results suggest that polyploid Artemia have evolved a suite of life-history characteristics adapting them to environments that contrast to those of their sympatric diploids. 相似文献
11.
Diploid (2n = 36) Tripsacum australe Cutler and Anderson var. hirsutum de Wet and Timothy, T. cundinamarce de Wet and Timothy, T. dactyloides (L.) L. var. dactyloides and var. meridonale de Wet and Timothy, and T. laxum Nash were crossed with Zea mays L. (2n = 20) as the pollen parent. True hybrids combine the cytologically nonreduced genome of Tripsacum (36 chromosomes) with the haploid (10 chromosomes) or more rarely diploid (20 chromosome) genome of Zea. Maternal offspring with 2n = 36 Tripsacum chromosomes commonly result from parthenogenetic development of cytologically nonreduced eggs. Some individuals with 2n = 36 Tripsacum chromosomes, however, resemble true hybrids in phenotype. These counterfeit hybrids incorporated Zea genetic material into their Tripsacum genomes without true fertilization having taken place. Offspring of counterfeit hybrids that were grown to maturity resembled their mothers in phenotype, and must have originated parthenogenetically. It is proposed that counterfeit hybrids are also produced in nature, and that this process contributes to origins of variation in gametophytic apomicts, and perhaps also in sexually reproducing species. 相似文献
12.
Chelone is a genus of perennial herbs comprising three diploid species (C. cuthbertii, C. glabra, and C. lyonii) and a fourth species (C. obliqua) that occurs as tetraploid and hexaploid races. To assess patterns of isozyme and morphological variation, and to test hypotheses of hybridization and allopolyploidy, we analyzed variation among 16 isozyme loci from 61 populations and 16 morphological characters from 33 populations representing all taxa and ploidy levels. Based on morphological analyses using clustering (unweighted pair group method using an arithmetic average) and ordination (principal components analysis and canonical variance analysis) methods, we recognize three diploid species without infraspecific taxa. Polyploids in the C. obliqua complex were most similar morphologically to diploid populations of C. glabra and C. lyonii. Patterns of isozyme variation among polyploids, which included fixed heterozygosity and recombinant profiles of alleles present in diploids, suggested polytopic origins of tetraploids and hexaploids. Our data indicate independent origins of polyploids in or near the southern Blue Ridge, Interior Highlands and Plains, and Atlantic Coastal Plain regions from progenitors most similar to C. glabra and C. lyonii. Extant tetraploids were not implicated in evolution of hexaploids, and plants similar to C. cuthbertii appeared unlikely as diploid progenitors for polyploids. We propose multiple differentiation and hybridization/polyploidization cycles in different geographic regions to explain the pattern of allopatry and inferred polytopic origins among polyploids. 相似文献
13.
Isozyme variation among 114 accessions of the Glycine tomentella Hayata was analysed by single linkage cluster analysis and the unweighted pair group centroid method (UPGMC). The diploid accessions fell into six distinct, well defined groups, which conformed with differences in chromosome number (2n − 38 or 2n − 40) or in geographic origin. The majority of the tetraploid accessions belonged to a large, geographically widespread group, predominantly aneuploid (2n − 78) group. The remaining four tetraploid groups were distinct on the basis of morphology or geographic distribution. The validity of tetraploid isozyme groupings for reflecting subspecific differentiation was supported by the published reports of hybrid fertility. All of the nineteen crosses between isozyme groups have yielded sterile hybrids, whereas five crosses within groups have yielded fertile hybrids. The relationship between diploids and tetraploids was examined either as the similarity between individual accessions, or that between isozyme groups. These analyses indicated that each tetraploid group is closely related to only one or two of the diploid groups or subgroups. 相似文献
14.
Diplosporous apomeiosis, formation of unreduced embryo sacs primarily of the Antennaria type, followed by parthenogenetic embryo development and pseudogamy (fertilization of the central cell) describe gametophytic apomixis within the Tripsacum agamic complex. Tripsacum dactyloides (Eastern gamagrass) is a close relative of domesticated maize and was chosen as a natural model system to investigate gene expression patterns associated with parthenogenesis. The genome size of diploid sexual and polyploid apomictic T. dactyloides was estimated by flow cytometry to be 7.37 pg (2C), 14.74 pg (4C) and 22.39 pg (6C), respectively. The diploid genome size is thus approximately 1.352 larger than that of maize. The apomeiotic-pseudogamous pathway of seed formation was demonstrated at a rate of 92% by the flow cytometric seed screen (FCSS) with single mature seeds in tetraploid accessions. This number includes twin embryos which were detected in 13% of the seeds analyzed. Fertilization of unreduced egg cells (BIII hybrids) was measured in 10% of apomictic seeds. Autonomous (fertilization-independent) embryo development and fertilization-dependent endosperm formation were confirmed by pollination of tetraploid T. dactyloides with a diploid transgenic maize line carrying an actin::#-glucuronidase (GUS) reporter construct. GUS expression was detected after pollination in the developing endosperm, but not in the embryo. In similar intraspecific crossing experiments with maize, GUS expression was detected in both the embryo and endosperm. A protocol was established for microdissection of embryo sacs and early parthenogenetic embryos of T. dactyloides. Together, these techniques provide new tools for future studies aimed at comparing gene expression patterns between sexual maize and sexual or apomictic T. dactyloides. 相似文献
15.
J. M. J. de Wet D. H. Timothy K. W. Hilu G. B. Fletcher 《American journal of botany》1981,68(2):269-276
The genus Tripsacum is widely distributed between 42°N and 24°S latitude. In South America, the genus extends around the Amazon and Orinoco basin, and from the Caribbean coast south to Brazil and Paraguay. The most common South American taxon is T. dactyloides (L.) L. var. meridonale de Wet and Timothy (2n = 36), which differs from North American representatives of the species in having subdigitate recemes usually appressed with the apical male sections typically curved. Closely related to T. dactyloides, but usually occupying more seasonally moist and dry habitats, is T. australe Cutler and Anderson. This species is typically robust with the basal leaf sheaths tomentose, and the much elongated culms becoming decumbent in older plants. Smaller plants, with essentially erect culms and leaf sheaths on the culms hirsute rather than tomentose, are recognized as T. australe var. hirsutum de Wet and Timothy. The two varieties of T. australe are both diploid (2n = 36) and they cross to produce fertile hybrids. They also cross with T. dactyloides var. meridonale (2n = 36), but these hybrids are partially sterile. Tripsacum cundinamarce de Wet and Timothy (2n = 36) is a robust species with glaucus leaves. It resembles robust specimens of T. dactyloides in having glabrous leaf sheaths, but can always be recognized by inflorescences that are composed of racemes arranged along a several-noded primary axis. This species is confined to moist habitats, while T. dactyloides occupies a range of habitats in South America. Tripsacum peruvianum de Wet and Timothy is a gametophytic apomict with 2n = 72, 90 or 108 chromosomes. It is characterized by an erect growth habit and strongly hirsute leaf sheaths. The cultivated Guatemala grass, T. andersonii Gray, occurs spontaneously in the mountains of Venezuela, Colombia, and Peru. This sexually sterile species is characterized by 2n = 64, and may combine 54 Tripsacum and 10 Zea chromosomes in its genome. Electrophoretic patterns of seed storage proteins confirm the validity of recognizing T. cundinamarce as distinct from T. dactyloides, and T. peruvianum as distinct from T. australe. 相似文献
16.
We examined genetic variation in sympatric diploid and polyploid brine shrimp Artemia parthenogenetica from each of three populations (China, Italy and Spain). Italian and Spanish tetraploids are closely related (I=0.964). Diploids and tetraploids within each of the two European populations are also closely related (mean I=0.905). Most alleles found in diploids also exist in sympatric polyploids. In contrast, the asexual Artemia (2N, 4N and 5N) in our study share few alleles with their close sexual relative, A. tunisiana (mean I=0.002). These results, as well as the work of other authors, strongly suggest that at least the tetraploid Artemia in our study have an autopolyploid origin.Clonal diversity of polyploid Artemia can be very high at least in some population. Both diploids and polyploids had low clonal diversities in the populations dominated by polyploids and high clonal diversities in the population dominated by diploids.The most common genotypes of sympatric diploid and polyploid Artemia frequently differed. Some alleles occurred only in diploids, while others were restricted to polyploids. These results suggest that polyploidy in Artemia has led to genetic divergence from diploid progenitors, and that ploidy-level variation must also be considered in developing an understanding of spatial and temporal allozyme polymorphism in asexual populations. 相似文献
17.
CHLOROPLAST DNA POLYMORPHISM AND PHYLOGENY IN THE B GENOME OF GLYCINE SUBGENUS GLYCINE (LEGUMINOSAE)
The B genome of Glycine subgenus Glycine comprises three diploid species whose monophyly is supported by morphological, crossing, and chloroplast DNA (cpDNA) data. Previous cpDNA studies indicated low levels of divergence among these taxa and failed to resolve cladistic relationships among them. More intensive studies of cpDNA variation were initiated, using additional restriction endonucleases and accessions. Results from cladistic analyses of over 50 restriction site characters indicate that there is considerable cpDNA polymorphism within this group of species, with a minimum of 27 plastome types occurring among the 74 accessions sampled. Levels of homoplasy observed in this group are relatively high (15%) for closely related congeneric species. There is only limited congruence between plastome type and taxonomic classification based on morphological characters. Explanations for this lack of concordance include: 1) the early state of taxonomic understanding in this group, 2) lack of resolution in the cpDNA tree caused by homoplasy and the small number of synapomorphic characters, 3) introgression among these interfertile, often sympatric taxa, and 4) maintenance of ancestral cpDNA polymorphisms resulting in shared plastomes among species. 相似文献
18.
Phylogenetic relationships between sympatric, morphologically indistinguishable diploid and tetraploid plants ofDactylis glomerata L. (Gramineae) in Galicia (Spain) were assessed using allozyme markers for 6 distinct systems. The study exploited recent introduction in Galicia and subsequent hybridization of an alien 4xDactylis subspecies possessing distinct allozymes from those of all the native plants. Opportunities for gene exchanges between the ploidies were estimated from in situ observations of flowering, examination of progenies in 2x/4x natural and experimental crosses, and enzyme analyses. Results show a high genetic similarity between the Galician diploids and tetraploids, which possess peculiar alleles in common. Although the ploidy levels usually have distinct flowering periods, interploidal crosses do occasionally occur. Gene flow is likely much more important from the diploid to the tetraploid level. A good genetic intermixing occurs between the Galician and the alien tetraploid entities which have simultaneous flowering. Autopolyploidization of the diploids followed by various rates of hybridization is proposed as one very probable origin of natural tetraploids inDactylis. 相似文献
19.
Jane Ellen Bradley John G. Carman Michelle S. Jamison Tamara N. Naumova 《Sexual plant reproduction》2007,20(1):9-17
One element of gametophytic apomixis is unreduced embryo sac (ES) formation, which often occurs precociously displacing or
replacing meiosis and causing apospory or diplospory, respectively. This study evaluated a premise that apomixis may evolve
in hybridogenous plants that contain duplicate sets of allelically divergent ovule development heterochrony genes. The duplicate
sets of genes would belong to duplicate genomic regions that are recombinationally isolated from each other (no gene flow)
by allopolyploidy or paleopolyploidy, and this isolation would genetically stabilize apomixis. For apomixis to evolve, the
ancestral donors of the duplicate regions must have differed from each other in timing of megasporogenesis, ES formation and
embryony such that epigenetic misexpressions, or competitions in expression, of the duplicate heterochrony genes in hybridogenous
derivatives would cause apomixis. Herein, we report substantial heterochrony in onset timing of germline stages among several
sexual diploid Tripsacum genotypes, which may have been progenitors of apomictic polyploid Tripsacum. Tripsacum floridanum and Tripsacum zopilotense genotypes entered meiosis early. The former advanced rapidly through ES formation, but the latter entered a lengthy lag phase
prior to ES formation. In two Tripsacum dactyloides var. dactyloides genotypes, meiosis occurred late and was followed by a distinct lag phase prior to ES formation. Likewise, the T. dactyloides var. meridonale genotype entered meiosis late, but the lag phase was brief. These differences appear to reflect allelic diversity at loci
responsible for onset timing of different germline development stages within and across species and possibly across the recombinationally
isolated duplicate chromosome regions in the Tripsacum paleopolyploid haplome (x = 18). Unique combinations of divergent alleles in hybridogenous plants coupled with polyploidy induced gene misexpressions
may be required for apomixis to evolve.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献