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1.
铁核桃根与叶水浸提液对果园间作绿肥植物的化感作用及其生理机制 总被引:1,自引:0,他引:1
以西南地区具有代表性的16种绿肥植物为受体材料,采用培养皿药膜法研究了铁核桃(Juglans sigillata)根水浸提液对受体种子发芽率及幼苗鲜重、干重的化感效应;并进一步研究了铁核桃根、叶水浸提液和胡桃醌对化感效应存在明显差异的4种绿肥植物(绿豆、红三叶、白三叶、花生)种子萌发与幼苗生长以及抗氧化酶特性的影响,以筛选适宜中国西南地区核桃园种植的绿肥植物,探讨核桃根和凋落物对绿肥作物的化感作用机制。结果表明:(1)铁核桃根水浸提液对绿豆的发芽率没有影响,但对绿豆幼苗鲜重和干重有显著抑制作用,而对其他15种绿肥的发芽率和鲜重、干重均有抑制作用。(2)胡桃醌显著抑制绿豆种子萌发,而铁核桃根或叶水浸提液对绿豆种子萌发没有影响。(3)铁核桃根或叶水浸提液以及胡桃醌对绿肥植物幼苗生长的化感效应趋势一致,但核桃根或叶水浸提液的化感效应强于胡桃醌。(4)绿豆幼苗在铁核桃根或叶水浸提液以及胡桃醌处理下,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性均高于其他3种(红三叶、白三叶、花生)受体幼苗,表明绿豆清除活性氧能力高,细胞受损害程度较低,受化感作用影响最弱。研究认为,绿豆为适宜中国西南地区幼龄核桃园种植的间作绿肥植物。 相似文献
2.
Ferulic acid impairs rhizogenesis and root growth,and alters associated biochemical changes in mung bean (Vigna radiata) hypocotyls 总被引:1,自引:0,他引:1
《Journal of Plant Interactions》2013,8(1):267-274
The present study investigated the effect of ferulic acid (FA; 0–1000 µM) on early growth, and rhizogenesis in mung bean (Vigna radiata) hypocotyls and associated biochemical changes. FA severely affected the radicle elongation and number of secondary roots after 72 h. The root and shoot length, number and length of secondary roots, and seedling dry weight of one-week-old seedlings of mung bean were decreased by 64%. The rooting potential (percent rooting, number and length of adventitious roots) of mung bean hypocotyls under in vitro conditions was significantly inhibited in response to 1–100 µM FA. At 1000 µM there was complete cessation of rooting. FA caused a reduction in the contents of water-soluble proteins and endogenous total phenolics, whereas the activities of proteases, peroxidases, and polyphenol peroxidases increased. The study concludes that FA inhibits root growth and development, and in vitro rooting process in mung bean by interfering with biochemical processes that are crucial for root formation. 相似文献
3.
Arthur Wallace 《Plant and Soil》1971,34(1):121-131
Summary When nonexchangeable absorption of Rb86, Na22, and Cs137 by bush bean (Phaseolus vulgaris L. var. Improved Tendergreen) was determined at different root temperatures and with and without Ca additions or pretreatments,
a strong interaction between temperature and Ca was observed. Ca inhibited Rb86 absorption markedly at low temperatures but had less effect on Cs137. Absorption of Na22 was inhibited by Ca at both low and high temperatures. Little effect for Ca with sometimes a Viets effect was observed at
high temperature for Rb but not for Cs or Na. Ratio pairs of Rb, Ca, and Na were used as an index of similarity of absorption
mechanisms. Cs and Rb, and Na and Rb appeared to be absorbed by different mechanisms at 10−3
M as indicated by temperature and Ca responses. Nitrate-N stimulated uptake of Rb only at high temperature with or without
Ca but not at low temperature. Ca in the pretreatment tended to result in greater long distance transport to shoots of Rb86 and Cs137 for the high temperature but Ca in the test solution slightly decreased the long distance transport. The data are discussed
in terms of the Viets effect and of a possible role of Ca in synthesis of transport proteins. 相似文献
4.
Paclobutrazol (PB) only slightly stimulated the rooting of mung bean cuttings but, interestingly, the number of adventitious roots formed was dramatically increased when PB was used together with indole-3-butyric acid (IBA). Application of PB in the first phase of root formation, when root initials are induced, caused the greatest enhancement of the promotive effect of IBA on rooting. Investigation of the effect of PB on uptake, transport and metabolism of [5-3 H]-IBA in mung bean cuttings revealed some changes in the rate of metabolism of IBA in comparison with control cuttings. PB was found to be involved in the partitioning of carbohydrates along the cuttings. Application of sucrose, like PB to the base of IBA-treated cuttings enhanced the effect of IBA. The patterns of the effects of PB and IBA, separately and together, on rooting were similar in defoliated and intact cuttings, however the number of roots was much lower in the defoliated cuttings, which lacked a source of assimilates. PB counteracted the effect of GA3 in the upper regions of the cuttings and seemed to increase the sink capacity at the base of the cuttings. The results of the present study clearly demonstrated the enhancing influence of PB on IBA stimulation of the rooting of mung bean cuttings. It is suggested that PB may affect the rate of metabolism of IBA during rooting and the status of the local sink, in the base of the cuttings, thus partially contributing to the enhancement of the rooting-promotive effect of IBA. 相似文献
5.
Stinemetz CL 《Plant Growth Regulation》1996,20(3):245-251
IAA responsiveness of sections of root tissue taken from the top and bottom of mung bean roots was assessed prior to and at varying times following gravistimulation. Prior to gravistimulation, root tissue sections from the sides of the elongation zone responded similarly to IAA. After gravistimulation (within 5 min), root sections from the bottom of the elongation zone became more responsive to IAA than sections collected from the upper side of the elongation zone. The change in IAA responsiveness of these tissue sections was transient with root sections from both the top and bottom of the elongation zone again exhibiting similar responsiveness to IAA following 15 minutes of gravistimulation.These studies also examined if the root tip is required for the gravity-induced shift in IAA responsiveness in the tissues of the elongation zone. The IAA responsiveness of top and bottom sections of the elongation zone from decapped mung bean roots was assessed at varying times following gravistimulation. The responsiveness to IAA of top and bottom sections changed rapidly in decapped roots, just as had been previously found for intact roots. Although the alteration in responsiveness was transient in decapped roots (just as intact roots), the time it took for the sections to recover previous responsiveness to IAA was extended.These results suggest that the initial growth response of graviresponding roots may be due to a change in the IAA responsiveness of tissues in the elongation zone and not an asymmetric accumulation of IAA on the lower side of the elongation zone. The results also indicate that the gravity-induced shift in IAA responsiveness in the elongation zone occurs independently of the root cap, suggesting that the cells in the elongation region can perceive and respond to gravity independently of the root cap during the intial phases of the gravity response. 相似文献
6.
Richard C. Smith Beverly H. St. John Rolando Parrondo 《American journal of botany》1973,60(9):839-845
Excised root segments of corn were subjected to osmotic stress by immersion in solutions of mannitol before, during, or after a period of Rb absorption. Both the time course of uptake (or loss) and selectivity of uptake were studied. Stress before or during the absorption period reduced Rb absorption to 20 % or less of that of controls, whereas it had no detectable influence on the constancy and selectivity of uptake. Stress imposed following a period of Rb accumulation caused root segments to lose only slightly more Rb than controls during the first 30 min, after which rates of change were insignificant in both. Segments stressed after the Rbabsorption period retained from 70–90 % as much Rb as did the controls, even after two hours. The comparatively great quantitative difference between effect of stress on uptake and its effect on loss is interpreted to mean that the mechanism of stress-induced reduction of ion absorption cannot be adequately explained on the sole basis of increased efflux of ions. 相似文献
7.
Summary The absorption and subsequent transport of foliar applied Rb86 labeled Rb Cl (10 mM) was studied on bean plants (Phaseolus vulgaris, L. cv. Black Seeded Blue Lake) exposed to physiologically tolerable levels of certain plant growth substances in the solution culture root media. Gibberellin A3 (10-5 M) increased Rb uptake but did not affect total translocation from the treated leaf. Translocation was directed to the upper vegetative parts and markedly reduced to the roots. Foliar influx of Rb and transport to the roots were greatly enhanced by 1-naphthaleneacetic acid (10-6 M) but mobilization of Rb into the leaves and upper stem was reduced. 2-Chloroethyltrimethylammonium chloride (10-3 M) and N,N-dimethylaminosuccinamic acid (3×10-4 M) decreased the mobility of Rb to the upper stem, increased it to the roots, and had no effect on initial uptake. Rb absorption was depressed by 2,4-dichlorobenzyltributylphosphonium chloride (10-5 M) with no effect on subsequent translocation. Both uptake and mobility were strikingly inhibited by N6-benzyladenine (10-6 M).These results suggest that absorption and the subsequent transport of foliar absorbed Rb are independent processes and that the distribution or mobilization of Rb in the various plant organs was not always a function of the chemically modified growth rate of the corresponding organ.Report No. COO-888-50 in cooperation with the Division of Biology and Medicine of the United States Atomic Energy Commission, Contract No. AT (11-1)-888. Journal Article No. 3643 of the Michigan Agricultural Experiment Station. 相似文献
8.
The effect of salt stress on polyamine biosynthesis and content in mung bean plants and in halophytes 总被引:9,自引:0,他引:9
The activity of L-arginine decarboxylase (EC 4.1.1.19) and L-ornithine decarboxylase (EC 4.1.1.17), polyamine content, and incorporation of arginine and ornithine into polyamines, were determined in mung bean [Vigna radiata (L.) Wilczek] plants subjected to salt (hypertonic) stress (NaCl at 0.51–2.27 MPa). Changes in enzyme activity in response to hypotonic stress were determined as well in several halophytes [Pulicaria undulata (L.), Kostei, Salsola rosmarinus (Ehr.) Solms-Laub, Mesembryanthemum forskahlei Hochst, and Atriplex halimus L.]. NaCl stress, possibly combined with other types of stress that accompanied the experimental conditions, resulted in organ-specific changes in polyamine biosynthesis and content in mung bean plants. The activity of both enzymes was inhibited in salt-stressed leaves. In roots, however, NaCl induced a 2 to 8-fold increase in ornithine decarboxylase activity. Promotion of ornithine decarboxylase in roots could be detected already 2 h after exposure of excised roots to NaCl, and iso-osmotic concentrations of NaCl and KCl resulted in similar changes in the activity of both enzymes. Putrescine level in shoots of salt-stressed mung bean plants increased considerably, but its level in roots decreased. The effect of NaCl stress on spermidine content was similar, but generally more moderate, resulting in an increased putrescine/spermidine ratio in salt-stressed plants. Exposure of plants to NaCl resulted also in organ-specific changes in the incorporation of both arginine and ornithine into putrescine: incorporation was inhibited in leaf discs but promoted in excised roots of salt-stressed mung bean plants. In contrast to mung bean (and several other glycophytes), ornithine and arginine decarboxylase activity in roots of halophytes increased when plants were exposed to tap water or grown in a pre-washed soil—i.e. a hypotonic stress with respect to their natural habitat. NaCl, when present in the enzymatic assay mixture, inhibited arginine and ornithine decarboxylase in curde extracts of mung bean roots, but did not affect the activity of enzymes extracted from roots of the halophyte Pulicaria. Although no distinct separation between NaCl stress and osmotic stress could be made in the present study, the data suggest that changes in polyamines in response to NaCl stress in mung bean plants are coordinated at the organ level: activation of biosynthetic enzymes concomitant with increased putrescine biosynthesis from its precursors in the root system, and accumulation of putrescine in leaves of salt-stressed plants. In addition, hypertonic stress applied to glycophytes and hypotonic stress applied to halophytes both resulted in an increase in the activity of polyamine biosynthetic enzymes in roots. 相似文献
9.
The effect of different cadmium concentrations (6–120 μM) on Hill reaction activity (HRA) of isolated chloroplasts, contents
of chlorophylls (Chls) and carotenoids (Cars), and Cd uptake and accumulation in plant organs of Indian mustard (Brassica juncea L. cv. Vitasso) and mung bean [Vigna radiata (L.) Wilczek] were determined. The Cd stress inhibited photochemical activity of isolated chloroplasts of both species and
in both tested developmental stages. On the basis of EC50 values, the mung bean showed a higher sensitivity to Cd treatment than Indian mustard. The higher sensitivity of both species
was determined in the earlier than in the older developmental stage. The leaves of Cd-treated plants possessed lower contents
of Chls and Cars in both species and the negative effect increased with Cd concentration. A difference between species was
also found in Cd uptake and accumulation. In both species, Cd was accumulated more in roots than in shoots, with higher accumulation
in Indian mustard than in mung bean. 相似文献
10.
We investigated the uptake of inorganic elements (Be, Na, Mg, K, Ca, Sc, Mn, Co, Zn, Se, Rb, Sr, Y, Zr, Ce, Pm, Gd, and Hf)
and the effect of Ca on their uptake in carrots (Daucus carota cv. U.S. harumakigosun) by the radioactive multitracer technique. The experimental results suggested that Na, Mg, K, and
Rb competed for the functional groups outside the cells in roots with Ca but not for the transporter-binding sites on the
plasma membrrane of the root cortex cells. In contrast, Y, Ce, Pm, and Gd competed with Ca for the transporters on the plasma
membrane. The selectivity, which was defined as the value obtained by dividing the concentration ratio of an elemental pair,
K/Na, Rb/Na, Be/Sr, and Mg/Sr, in the presence of 0.2 and 2 ppm Ca by that of the corresponding elemental pair in the absence
of Ca in the solution was estimated. The selectivity of K and Rb in roots was increased in the presence of Ca. The selectivity
of Be in roots was not affected, whereas the selectivity of Mg was increased by Ca. These observations suggest that the presence
of Ca in the uptake solution enhances the selectivity in the uptake of metabolically important elements against unwanted elements. 相似文献
11.
人参、西洋参能通过根系分泌三萜皂苷等化感物质,严重影响后茬人参的生长,但对人参以外的植物是否具有化感效应尚不清楚。本实验研究了不同质量浓度的人参皂苷对小麦、白菜、黄瓜及绿豆4种常见栽培作物早期根系发育的影响,结果发现人参皂苷处理液(25、50和100mg·L-1)对4种作物主根及不定根的发育影响不尽相同。随着处理质量浓度的升高,小麦、白菜、黄瓜根系活力分别比同组CK明显降低,根长、根鲜重也呈降低趋势。各浓度人参皂苷处理对黄瓜和绿豆下胚轴不定根的数量、根长、根鲜重及根系活力的影响均未达到显著水平,但二者抗氧化酶的活性都微有升高。总之,人参皂苷对4种栽培作物的主根发育均有抑制作用,尤其对小麦、黄瓜主根生长的抑制作用较强;但对黄瓜和绿豆不定根发育的影响不明显。 相似文献
12.
人参、西洋参能通过根系分泌三萜皂苷等化感物质,严重影响后茬人参的生长,但对人参以外的植物是否具有化感效应尚不清楚.本实验研究了不同质量浓度的人参皂苷对小麦、白菜、黄瓜及绿豆4种常见栽培作物早期根系发育的影响,结果发现人参皂苷处理液(25、50和100 mg· L-1)对4种作物主根及不定根的发育影响不尽相同.随着处理质量浓度的升高,小麦、白菜、黄瓜根系活力分别比同组CK明显降低,根长、根鲜重也呈降低趋势.各浓度人参皂苷处理对黄瓜和绿豆下胚轴不定根的数量、根长、根鲜重及根系活力的影响均未达到显著水平,但二者抗氧化酶的活性都微有升高.总之,人参皂苷对4种栽培作物的主根发育均有抑制作用,尤其对小麦、黄瓜主根生长的抑制作用较强;但对黄瓜和绿豆不定根发育的影响不明显. 相似文献
13.
The influence of membrane surface charge on cation uptake was investigated in protoplasts prepared from roots of mung bean (Vigna radiata L.). Confocal laser scanning microscopy showed that a fluorescent trivalent cation accumulated to very high concentrations at the surface of the protoplasts when they were incubated in medium containing low concentrations of Ca or other cations, but that this accumulation could be completely reversed by suppression of membrane surface negativity by high cation concentrations. Influx of 63Ni was strongly reduced by a range of divalent cations. Increasing the Ca concentration in the medium from 25 microM to 10 mM inhibited 63Ni influx by more than 85%. 63Ni influx was also inhibited by 85% by reducing the pH from 7 to 4. Computation of the activity of Ni at the membrane surface under the various treatment conditions showed that Ni uptake was closely correlated with its activity at the membrane surface but not with its concentration in the bulk medium. It was concluded that the effects on Ni uptake of addition of monovalent, divalent and trivalent cations, and of variations in pH are all consistent with the proposition that the activity of Ni at the membrane surface is the major determinant of the rate of Ni influx into mung bean protoplasts. It is proposed that the surface charge on the plasma membrane will influence the membrane transport of most charged molecules into cells. 相似文献
14.
Comparison of movement and metabolism of indole-3-acetic acid and indole-3-butyric acid in mung bean cuttings 总被引:2,自引:0,他引:2
Indole-3-butyric acid (IBA) was much more effective than indole-3-acetic acid (IAA) in inducing adventitious root formation in mung bean ( Vigna radiata L.) cuttings. Prolonging the duration of treatment with both auxins from 24 to 96 h significantly increased the number of roots formed. Labelled IAA and IBA applied to the basal cut surface of the cuttings were transported acropetally. With both auxins, most radioactivity was detected in the hypocotyl, where roots were formed, but relatively more IBA was found in the upper sections of the cuttings. The rate of metabolism of IAA and IBA in these cuttings was similar. Both auxins were metabolized very rapidly and 24 h after application only a small fraction of the radioactivity corresponded to the free auxins. Hydrolysis with 7 M NaOH indicates that conjugation is the major pathway of IAA and IBA metabolism in mung bean tissues. The major conjugate of IAA was identified tentatively as indole-3-acetylaspartic acid, whereas IBA formed at least two major conjugates. The data indicate that the higher root-promoting activity of IBA was not due to a different transport pattern and/or a different rate of conjugation. It is suggested that the IBA conjugates may be a better source of free auxin than those of IAA and this may explain the higher activity of IBA. 相似文献
15.
E. Levi 《Physiologia plantarum》1968,21(1):213-226
The initial and subsequent distribution of 22Na, 43K, 86Rb, 134Cs, 32P, 35S, 45Ca, 65Zn and 85Sr in bean plants, following leaf and root uptake was studied under controlled environmental conditions. Autoradiographic and counting methods reveal vascular connections between leaves and roots and two patterns of distribution in the leaves following root uptake are reported. Distribution following treatment of specific parts of the leaves or roots points to a morphological unity in the bean plant. A possible circulation pattern for some ions is discussed. 相似文献
16.
Takuma Tanada 《Planta》1978,143(1):109-111
Hypocotyl sections from dark-grown mung bean (Vigna radiata (L.) Wilczek) seedlings grown without boron exhibit little or no increase in the translocation of fluorescein in phloem cells following gravitational stimulation or red irradiation. In contrast, pretreatment of the hypocotyl sections with low concentrations of boric acid before red irradiation or gravitational stimulation results in a rapid increase in fluorescein movement. Mung bean root tips without boron fail to remain attached to a negativelycharged glass surface after several minutes of red irradiation. A low boron concentration in the solution induces tip attachment. The suggestion is made that boron is required to stabilize a positive electrostatic charge in the plasma membrane that is generated by the actions of phytochrome and gravity. 相似文献
17.
Aluminum Effects on Calcium (45Ca2+) Translocation in Aluminum-Tolerant and Aluminum-Sensitive Wheat (Triticum aestivum L.) Cultivars (Differential Responses of the Root Apex versus Mature Root Regions) 总被引:2,自引:2,他引:0
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The influence of Al exposure on long-distance Ca2+ translocation from specific root zones (root apex or mature root) to the shoot was studied in intact seedlings of winter wheat (Triticum aestivum L.) cultivars (Al-tolerant Atlas 66 and Al-sensitive Scout 66). Seedlings were grown in 100 [mu]M CaCl2 solution (pH 4.5) for 3 d. Subsequently, a divided chamber technique using 45Ca2+-labeled solutions (100 [mu]M CaCl2 with or without 5 or 20 [mu]M AlCl3, pH 4.5) was used to study Ca2+ translocation from either the terminal 5 to 10 mm of the root or a 10-mm region of intact root approximately 50 mm behind the root apex. The Al concentrations used, which were toxic to Scout 66, caused a significant inhibition of Ca2+ translocation from the apical region of Scout 66 roots. The same Al exposures had a much smaller effect on root apical Ca2+ translocation in Atlas 66. When a 10-mm region of the mature root was exposed to 45Ca2+, smaller genotypic differences in the Al effects effects on Ca2+ translocation were observed, because the degree of Al-induced inhibition of Ca2+ translocation was less than that at the root apex. Exposure of the root apex to Al inhibited root elongation by 70 to 99% in Scout 66 but had a lesser effect (less than 40% inhibition) in Atlas 66. When a mature root region was exposed to Al, root elongation was not significantly affected in either cultivar. These results demonstrate that genotypic differences in Al-induced inhibition of Ca2+ translocation and root growth are localized primarily in the root apex. The pattern of Ca2+ translocation within the intact root was mainly basipetal, with most of the absorbed Ca2+ translocated toward the shoot. A small amount of acropetal Ca2+ translocation from the mature root regions to the apex was also observed, which accounted for less than 5% of the total Ca2+ translocation within the entire root. Because Ca2+ translocation toward the root apex is limited, most of the Ca2+ needed for normal cellular function in the apex must be absorbed from the external solution. Thus, continuous Al disruption of Ca2+ absorption into cells of the root apex could alter Ca2+ nutrition and homeostasis in these cells and could play a pivotal role in the mechanisms of Al toxicity in Al-sensitive wheat cultivars. 相似文献
18.
19.
Ten phenolic compounds were examined for their effect on mung bean (Phaseolus aureus L.) hypocotyl growth and on respiration and coupling parameters of isolated mung bean hypocotyl mitochondria. Three compounds—tannic, gentisic, and p-coumaric acids—inhibited hypocotyl growth and when incubated with isolated hypocotyl mitochondria released respiratory control, inhibited respiration, and prevented substrate-supported Ca2+ and PO4 transport. Vanillic acid also inhibited hypocotyl growth and reduced mitochondrial Ca2+ uptake but did not affect respiration or respiratory control of isolated mitochondria. This is the first compound reported to selectively inhibit Ca2+ uptake in plant mitochondria. Two other phenolic compounds—α, 3,5-resorcylic and protocatechuic acids—showed no significant effect on hypocotyl growth and did not affect mitochondrial oxidative phosphorylation either separately or in various combinations. Four phenolic compounds—ferulic, caffeic, p-hydroxybenzoic, and syringic acids—showed a significant reduction in mung bean hypocotyl growth but did not inhibit any of the mitochondrial processes examined. The results show that phenolic compounds which alter respiration or coupling responses in isolated mitochondria also inhibit hypocotyl growth and may reflect a mechanism of action for these natural growth inhibitors. 相似文献
20.
Changes in Root Resistance as a Function of Applied Suction, Time of Day, and Root Temperature 总被引:3,自引:0,他引:3
Water uptake rate of decapitated root systems of cotton (Gossypium hirsutum L.), tomato (Lycopersicon esculentum L. cv. Rutgers), and kidney bean (Phaseolus vulgaris L.) plants shows an exponential increase with applied suction up to about —1 bar. The water uptake rate was higher on the descending path of applied suction than on the ascending path, indicating a hysteresis effect in the roots. The root resistance in a cotton plant increased between 3-to 5-fold during the photoperiod of 12 hours. The water uptake rate increased with increasing temperature of the root medium up to 30°C in cotton and 25°C in tomato and bean plants. 相似文献