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Low-temperature-induced desaturation of fatty acids and expression of desaturase genes in the cyanobacterium Synechococcus sp. PCC 7002 总被引:1,自引:0,他引:1
Toshio Sakamoto Shoichi Higashi Hajime Wada Norio Murata Donald A. Bryant 《FEMS microbiology letters》1997,152(2):313-320
Changes in response to temperature of lipid classes, fatty acid composition and mRNA levels for acyl-lipid desaturase genes were studied in the marine unicellular cyanobacterium, Synechococcus sp. PCC 7002. The degree of unsaturation of C18 fatty acids increased in cells grown at lower temperature for all lipid classes, and ω3 desaturation occurred specifically in cells grown at low temperature. While the level of 18:1(9) fatty acids declined, desaturation at the ω3 position of C18 fatty acids increased gradually during a 12-h period after a temperature shift-down to 22°C. However, the mRNA levels of the desA (Δ12 desaturase), desB (ω3 desaturase) and desC (Δ9 desaturase) genes increased within 15 min after a temperature shift-down to 22°C; the desaturase gene mRNA levels also rapidly declined within 15 min after a temperature shift-up to 38°C. Therefore, the elevation of mRNA levels for the desaturase genes is not the rate-limiting event for the increased desaturation of membrane lipids after a temperature shift-down. The rapid, low-temperature-induced changes in mRNA levels occurred even when cells were grown under light-limiting conditions for which the growth rates at 22°C and 38°C were identical. These studies indicate that the ambient growth temperature, and not some other growth rate-related process, regulates the expression of acyl lipid desaturation in this cyanobacterium. 相似文献
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Cheevadhanarak S Paithoonrangsarid K Prommeenate P Kaewngam W Musigkain A Tragoonrung S Tabata S Kaneko T Chaijaruwanich J Sangsrakru D Tangphatsornruang S Chanprasert J Tongsima S Kusonmano K Jeamton W Dulsawat S Klanchui A Vorapreeda T Chumchua V Khannapho C Thammarongtham C Plengvidhya V Subudhi S Hongsthong A Ruengjitchatchawalya M Meechai A Senachak J Tanticharoen M 《Standards in genomic sciences》2012,6(1):43-53
Arthrospira platensis is a cyanobacterium that is extensively cultivated outdoors on a large commercial scale for consumption as a food for humans and animals. It can be grown in monoculture under highly alkaline conditions, making it attractive for industrial production. Here we describe the complete genome sequence of A. platensis C1 strain and its annotation. The A. platensis C1 genome contains 6,089,210 bp including 6,108 protein-coding genes and 45 RNA genes, and no plasmids. The genome information has been used for further comparative analysis, particularly of metabolic pathways, photosynthetic efficiency and barriers to gene transfer. 相似文献
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Dauenpen Meesapyodsuk Darwin W. Reed Supapon Cheevadhanarak Patcharaporn Deshnium Patrick S. Covello 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2001,129(4)
The initial and rate determining step in the mechanism of fatty acid desaturases has been proposed to be breakage of one of the C---H bonds at the site of the incipient double bond. This has been investigated and supported for a number of eukaryotic fatty acid desaturases through the use of kinetic isotope effect experiments with deuterated substrates. In order to probe the reaction catalyzed by the cyanobacterial Δ9 desaturase and compare it to the eukaryotic desaturases, the desC gene of Spirulina platensis, strain C1 (Arthrospira sp. PCC 9438) was expressed in a desaturase mutant of baker's yeast. Kinetic isotope effects were performed by culturing yeast transformants with deuterated thia-substituted stearic acids. A large kinetic isotope effect was found for the 9 position, in qualitative agreement with results from eukaryotic desaturases. 相似文献
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Photosynthetic and respiratory electron transport and their interplay with ion transport have been studied in Arthrospira platensis, a filamentous alkaliphilic cyanobacterium living in hypersaline lakes. As typical for alkaliphiles, A. platensis apparently does not maintain an outward positive pH gradient at its plasma membrane. Accordingly, sodium extrusion occurs
via an ATP-dependent primary sodium pump, in contrast to the Na+/H+ antiport in most cyanobacteria. A. platensis is strongly dependent on sodium/bicarbonate symport for the uptake of inorganic carbon. Sodium extrusion in the presence
of the Photosystem II inhibitor diuron indicates that a significant amount of ATP is supplied by cyclic electron transport
around Photosystem I, the content of which in A. platensis is exceptionally high. Plastoquinol is oxidized by two parallel pathways, via the cytochrome b
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f complex and a putative cytochrome bd complex, both of which are active in the light and in the dark.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Uptake rates of macrominerals and trace elements were characterized in batch and continuous cultures of Spirulina platensis under photoautotropic conditions. The values of yield coefficients were determined using inductively coupled plasma emission spectroscopy (ICP-ES). Further simplifications of culture medium proved possible, mainly in the trace element solutions; concentrations of some elements were lowered and trace elements B, Mo, V, Cr, Ni, Co, W, and Ti were removed. 相似文献
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Mostafa M. S. Ismaiel Yassin M. El-Ayouty Peter C. Loewen Michele D. Piercey-Normore 《Journal of applied phycology》2014,26(4):1649-1658
Superoxide dismutase (SOD) is considered a primary antioxidant which defends against reactive oxygen species that are induced by environmental stress. In this study, we examined changes in SOD activity and expression in the cyanobacterium Spirulina (Arthrospira) platensis under iron and salinity stress; we characterized its induction under these stress conditions and we overexpressed the enzyme in a bacterial host for preliminary characterization. Analysis of SOD isoforms concludes that S. platensis was found to regulate only the iron-containing SOD isoform (FeSOD) in response to two types of stress that were tested. The FeSOD expression (on the level of both mRNA and enzyme activity) was induced by the stress conditions of salinity and iron levels. The FeSOD from S. platensis was overexpressed in Escherichia coli BL21. The recombinant FeSOD protein (about 23 kDa) was purified for characterization. It showed high specific activity and pH stability at 6.0–9.0, and it is relatively thermostable, retaining 45 % of its activity after 30 min at 90 °C. Phylogenetic analysis reveals that S. platensis FeSOD is grouped with the FeSODs from other cyanobacterial species and separated from those of the eukaryotic Chlorophyta, suggesting that the FeSOD gene may be used as a molecular marker in physiological, phylogenetic, and taxonomic studies. This study also suggests that the increased activity and expression of SOD may play a role in algal survival under stress conditions. 相似文献
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Abstract Sequences homologous to the structural genes for dinitrogenase ( nifD and nifK ) and nitrogenase reductase ( nifH ) have been cloned from the filamentous, non-heterocystous cyanobacterium Pseudanabaena PCC7409. The nifHDK − homologous sequences were shown to reside on a 6.5-kb Eco RI restriction fragment by using a restriction fragment encoding the Klebsiella pneumoniae nifHDK genes as a heterologous hybridization probe. This 6.5-kb restriction fragment was cloned from a λ gt.wes Eco RI library of the Paseudanabaena sp. PCC7409 genome. This fragment was subcloned into the plasmid vector pUC9 to generate plasmid pPSU20. A detailed physical map of the insert in plasmid pPSU20 was determined, and relative positions of the nifH, nifD , and nifK homologous sequences on this fragment were determined by hybridization analysis with gene-specific fragments derived from the corresponding Anabaena sp. PCC7120 genes. The results indicate that these genes are contiguous in Pseudanabaena sp. PCC 7409 and are arranged in the order nifH, nifD , and nifK . This arrangement resembles that observed for other non-heterocystous cyanobacteria but differs from that observed for Anabaena, Calothrix , and Nostoc species. 相似文献
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Buoyancy provided by gas vesicles has been suggested to play an important role in regulating vertical distribution and nutrient acquisition in cyanobacteria. However, little is known about how changes in UV radiation (UVR, 280–400 nm) would affect the buoyancy. We have shown here that the floatation activity of the economically important cyanobacterium Arthrospira platensis (D-0083) decreased with increased photosynthetic rates associated with increased photosynthetically active radiation (PAR), but it decreased less in the presence of UVR, which resulted in inhibitory effects. When the cells were grown under isoenergetic levels of solar PAR or UVR alone, they migrated downward under PAR but maintained buoyant under UVR. The buoyancy regulation of A. platensis depended on the exposed levels of PAR as well as UVR, which affected photosynthesis and growth in an antagonistic way. The buoyancy of A. platensis in water columns is likely to be dependant on diurnal photosynthetic performance regulated by solar radiation, and can hardly be considered as an active strategy to gain more energy during sunrise/sunset or to escape from harmful irradiation during the noon period. 相似文献
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Urea increases fed‐batch growth and γ‐linolenic acid production of nutritionally valuable Arthrospira (Spirulina) platensis cyanobacterium
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Carlos Eduardo Nascimento Sassano Luiz Antonio Gioielli Attilio Converti Iracema de Oliveira Moraes Sunao Sato João Carlos Monteiro de Carvalho 《Engineering in Life Science》2014,14(5):530-537
In this article we investigate the simultaneous influence of feeding time and amount of urea added as a nitrogen source on the fed‐batch growth and composition of Arthrospira (Spirulina) platensis. Cultivations were performed in 5‐L minitanks at constant temperature (25°C) and light intensity (42 μmol photons/m2s), using exponentially increasing rate of urea addition, and varying the above independent variables in the ranges 9–15 days and 4.6–12.1 mM, respectively. Special emphasis was placed on the content of added high value fatty acids (e.g., γ‐linolenic acid) of concern for the food industry. To this purpose, a 22‐plus star central composite design was employed, and maximum cell concentration, cell productivity, yield of biomass on nitrogen added, protein content and fatty acids profile were evaluated by multiple regression analysis. The highest cell concentration (1759 mg/L) was obtained at feeding time of 14 days and amount of urea per unit reactor volume of 5.8 mM, while the highest contents of γ‐linolenic acid (27.5% of the lipid fraction) and proteins (77.2%) were obtained at 10 and 14 days and 5.8 and 10.8 mM, respectively. The results confirm the possibility of using urea as cheap nitrogen source to culture this nutritionally valuable cyanobacterium. 相似文献
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Yohei Kurio Yosuke Koike Yu Kanesaki Satoru Watanabe Shigeki Ehira 《Molecular microbiology》2020,114(4):553-562
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Differential regulation of ssb genes in the nitrogen‐fixing cyanobacterium,Anabaena sp. strain PCC71201
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Anabaena sp. PCC7120 possesses three genes coding for single‐stranded DNA‐binding (SSB) protein, of which ssb1 was a single gene, and ssb2 and ssb3 are the first genes of their corresponding operons. Regulation of the truncated ssb genes, ssb1 (alr0088) and ssb2 (alr7559), was unaffected by N‐status of growth. They were negatively regulated by the SOS‐response regulatory protein LexA, as indicated by the (i) binding of Anabaena LexA to the LexA box of regulatory regions of ssb1 and ssb2, and (ii) decreased expression of the downstream gfp reporter gene in Escherichia coli upon co‐expression of LexA. However, the full‐length ssb gene, ssb3 (all4779), was regulated by the availability of Fe2+ and combined nitrogen, as indicated by (i) increase in the levels of SSB3 protein on Fe2+‐depletion and decrease under Fe2+‐excess conditions, and (ii) 1.5‐ to 1.6‐fold decrease in activity under nitrogen‐fixing conditions compared to nitrogen‐supplemented conditions. The requirement of Fe2+ as a co‐factor for repression by FurA and the increase in levels of FurA under nitrogen‐deficient conditions in Anabaena (Lopez‐Gomollon et al. 2007) indicated a possible regulation of ssb3 by FurA. This was substantiated by (i) the binding of FurA to the regulatory region of ssb3, (ii) repression of the expression of the downstream gfp reporter gene in E. coli upon co‐expression of FurA, and (iii) negative regulation of ssb3 promoter activity by the upstream AT‐rich region in Anabaena. This is the first report on possible role of FurA, an important protein for iron homeostasis, in DNA repair of cyanobacteria. 相似文献
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Effect of temperature and growth phase on fatty acid composition of the psychrophilic Vibrio sp. strain no. 5710 总被引:2,自引:0,他引:2
Tetsuo Hamamoto Nobuhisa Takata Toshiaki Kudo Koki Horikoshi 《FEMS microbiology letters》1994,119(1-2):77-81
Abstract The cellular fatty acid composition of the psychrophilic Vibrio sp. strain No. 5710 isolated from a deep-sea sediment sample was analyzed. The presence of docosahexaenoic acid (22:6) was demonstrated as found previously in other deep-sea bacteria, and the relative amount of 22:6 decreased as the growth temperature increased. A temperature shift from 10°C to 0°C resulted in a relative increase of 22:6, and an opposite shift led to a decrease. In addition, hexadecanoic acid (16:0) was found to increase as the growth temperature increased. Therefore, it is suggested that the adaptation of 5710 to the growth temperature was carried out by the changes in the relative amounts of 22:6 and 16:0. When 5710 was grown at low temperature, it increased the relative amount of 22:6 presumably to maintain membrane fluidity at that temperature. In contrast, 5710 grown at high temperature probably maintained the membrane fluidity by increasing the amount of a saturated fatty acid, 16:0. Furthermore, observation of the fatty acid compositions at mid-exponential phase and early stationary phase revealed the proportions of several fatty acids, including a major fatty acid, 9- cis -hexadecenoic acid (16:1c, palmitoleic acid), were affected by the growth phase which may be due to the physiological difference between the growth phases. 相似文献
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将钝顶螺旋藻培养在含有不同NaCl浓度(0、0.4、0.8mol·L^-1)的培养基中,并置于室外全波段太阳辐射、阳光辐射滤除uvB以及光合有效辐射(PAR)三种辐射条件下,以探讨阳光uV辐射和盐胁迫对钝顶螺旋藻的耦合效应。结果表明,阳光uv辐射显著抑制钝顶螺旋藻的光化学效率,且随着盐浓度的提高,其受抑制程度加剧。D1蛋白含量在高水平PAR和uV辐射下都明显降低,而高盐浓度(0.8mol·L^-1NaCl)导致其含量进一步下降。此外,阳光uv辐射与盐胁迫的耦合作用使得藻丝发生明显断裂。 相似文献