Phylogenetic analysis of the Diplomonadida (Wenyon, 1926) Brugerolle, 1975: evidence for heterochrony in protozoa and against Giardia lamblia as a "missing link".

A suite of 23 ultrastructural characters was used in a phylogenetic analysis of the protozoan order Diplomonadida. A single most parsimonious solution was found, with a length of 38 transformations and a consistency index of 0.84. The cladogram supports previous hypotheses of the relationships of the genera in the suborder Diplomonadina, as well as the inclusion of the genera Enteromonas and Trimitus in the order. Heterochrony is suggested in the change to binary axial symmetry, as hypermorphosis resulting from delayed cytokinesis in the ancestor. Hypotheses regarding a pivotal position for Giardia lamblia in the evolution of eukaryotes are inconsistent with the phylogeny proposed here.     

A synopsis of gall midges associated with conifers,with description of a new species of the genus <Emphasis Type="Italic">Kaltenbachiola</Emphasis> Hedicke (Diptera,Cecidomyiidae: Lasiopterinae) from Yakutia,damaging cones of the Siberian spruce (<Emphasis Type="Italic">Picea obovata</Emphasis>)

The world fauna comprises 134 species from 52 genera of gall midges associated with plants of the order Pinales (3 families, 16 genera and 74 species); 14 genera are specific to Pinales. The distribution of genera and species of gall midges over host plant taxa is described. The Holarctic genus Kaltenbachiola comprises four species all developing in the spruce (Picea spp.) cones. Earlier, K. strobi (Winnertz), widely distributed in Europe, was the only species known in the Palaearctic. A new species Kaltenbachiola anastasiae sp. n. is described, which damages cones of Picea obovata in Central Yakutia. The phylogenetic relationships of Kaltenbachiola with close genera specific to Pinales are characterized, and an updated diagnosis of the genus with additional morphometric parameters and keys to the pine-specific genera of the tribe Dasineurini and to species of the genus Kaltenbachiola are given. The host associations and specific traits of biology and distribution of Kaltenbachiola species are considered.     

Systematic position and composition of the superfamily Notanoplioidea Gill, 1969 (Brachiopoda, Articulata)

The systematic position of several genera of articulate brachiopods grouped around Notanoplia Gill is controversial. Notanoplia was established in 1950 within the order Chonetida; this genus and several externally similar genera, which were established later, were referred by different authors to the orders Orthida, Strophomenida, Chonetida, Atrypida, Spiriferida, or considered as incertae sedis. The morphological analysis of the shell of Notanoplia and the related genera Boucotia and Costanoplia supports the placement of these genera in a separate superfamily, Notanoplioidea, within the order Strophomenida. Other genera similar in the shell outline and shape to Notanoplia and conditionally considered as related to it probably belong to other strophomenid superfamilies or other orders.     

周口店更新世鸟类化石

<正> 北京市房山县周口店更新世洞穴,尤其北京猿人产地的第一地点(Loc.1)富含鸟类化石。自1927年开始至1980年采获的鸟类化石多达万件。同北京猿人相伴生的周口店脊椎动物群,是我国北方第四纪动物群的主要代表。近半个世纪来,中外学者,除对周口店人类化石、文化遗迹等进行过不少详细的研究,也对脊椎动物化石,主要是哺     

Effectiveness of imazalil to control the effect of fungal deterioration on mummies at the Mexico City Museum "El Carmen"

We present a study on the control and elimination of the fungi affecting the mummies specifically at the museum "El Carmen", in San Angel, Mexico City. Twelve analysed mummies presented an important deterioration attributed to colonizing fungi. The degree of fungal contamination and the efficacy of imazalil were evaluated. Two samplings were performed in order to isolate and identify the fungal genera, one for control and the other after the treatment. Isolation was done by the carpet-square technique and identification was performed by morphological features. Each sampling gave a total of 100 samples as follows: 17 from the air, 23 from the walls and 60 from the mummies. Samples were cultured on Sabouraud dextrose agar. From the first sampling a total of 649 colonies corresponding to 24 genera were obtained being the most frequent Penicillium, Cladophialophora and Aspergillus. From the second sampling, after the imazalil treatment, which was applied by means of lit candles containing the antifungal drug, 57 colonies were recovered, representing a 91.2% fungal reduction; 18 genera were eliminated. In spite of resistance showed by many Penicillium strains, the imazalil is an alternative drug for the control of fungal colonization on these studied materials.     

Hidden generic diversity in Neotropical birds: molecular and anatomical data support a new genus for the "Scytalopus"indigoticus species-group (Aves: Rhinocryptidae)

The genus Scytalopus is a species-rich and taxonomically complicated component of the Neotropical avian family Rhinocryptidae. Probably because Scytalopus is a superficially uniform assemblage, its monophyly has not been seriously questioned. We investigated phylogenetic relationships of a representative set of species in the genus using nuclear and mitochondrial DNA sequences as well as anatomical data, and provided the first test of its presumed monophyly by including in the analyses its hypothesized closest relatives (the genera Myornis, Eugralla, and Merulaxis) as well as most rhinocryptid genera. We found strong support for the paraphyly of the genus Scytalopus, with the Scytalopus indigoticus species-group forming a clade with Merulaxis. A well-supported clade including the genera Eugralla, Myornis, and the remaining Scytalopus was also recovered. Because these results were recovered independently and with strong support using mitochondrial and nuclear data, and were entirely consistent with anatomical data, we erect a new genus for the S.indigoticus species-group. These findings illustrate the importance of formally testing hypotheses of monophyly even for well-accepted groups of Neotropical birds.     

The Order Euplotida (Ciliophora): Taxonomy, with Division of Euplotes into Several Genera

ABSTRACT. The order Euplotida represents a monophyletic order of five families of hypotrich ciliates united by morphology, stomatogenesis, ultrastructure, cyst structure, and behavior. A review of variability of ciliation and nuclei among the 14 genera suggests that lines of evolution may have involved both the loss of cirri and nuclear simplification. We present a binary key to genera in the families Aspidiscidae ( Aspidisca and Euplotaspis ), Certesiidae n. fam. ( Certesia ), Gastrocirrhidae ( Cytharoides, Euplotidium , and Gas-trocirrhus ), Uronychiidae ( Diophryopsis, Diophrys, Paradiophrys , and Uronychia ), and Euplotidae. The latter family contains species formerly in the genus Euplotes. Based primarily on cortical structure, endosymbionts, data from morphometric analysis, and ecology, we recognize four different groups. The first group of species remains in Euplotes with Euplotes charon as type. We place a second group of species into the genus Moneuplotes Jankowski 1979 with Moneuplotes vannus (Müller, 1786) as type. We erect two new genera: Euplotoides n. g. and Euplotopsis n. g. with Euplotoides patella (Müller, 1773) n. comb. and Euplotopsis affinis (Dujardin, 1841) n. comb. as type species respectively. We discuss possible phylogenetic relationships within the order.     

Granulosicoccaceae fam. nov., to include Granulosicoccus antarcticus gen. nov., sp. nov., a non-phototrophic, obligately aerobic chemoheterotroph in the order Chromatiales, isolated from Antarctic seawater

A Gram-negative, motile by tuft flagella, obligately aerobic chemoorganoheterotrophic, sphere-form bacterium, designated IMCC3135(T), was isolated from the Antarctic surface seawater of King George Island, West Antarctica. The strain was mesophilic, neutrophilic, and requiring NaCl for growth, but neither halophilic nor halotolerant. The 16S rRNA gene sequence analysis indicated that the strain was most closely related to genera of the order Chromatiales in the class Gammaproteobacteria. The most closely related genera showed less than 90% 16S rRNA gene sequence similarity and included Thioalkalispira (89.9%), Thioalkalivibrio (88.0%-89.5%), Ectothiorhodospira (87.9%-89.3%), Chromatium (88.3%-88.9%), and Lamprocystis (87.7%-88.9%), which represent three different families of the order Chromatiales. Phylogenetic analyses showed that this Antarctic strain represented a distinct phylogenetic lineage in the order Chromatiales and could not be assigned to any of the defined families in the order. Phenotypic characteristics, including primarily non-phototrophic, non-alkaliphilic, non-halophilic, and obligately aerobic chemoheterotrophic properties, differentiated the strain from other related genera. The very low sequence similarities (<90%) and distant relationships between the strain and members of the order suggested that the strain merited classification as a novel genus within a novel family in the order Chromatiales. On the basis of these taxonomic traits, a novel genus and species is proposed, Granulosicoccus antarcticus gen. nov., sp. nov., in a new family Granulosicoccaceae fam. nov. Strain IMCC3135(T) (=KCCM 42676(T)=NBRC 102684(T)) is the type strain of Granulosicoccus antarcticus.     

Morphological and Taxonomic Interpretation of <Emphasis Type="Italic">Orestovia</Emphasis> and <Emphasis Type="Italic">Schuguria</Emphasis>, Enigmatic Devonian Plants from Russia

A detailed study of the external and internal surfaces of the cuticle of Orestovia and Schuguria using scanning electron microscopy and confocal laser microscopy has shown that the structures formerly interpreted as stomata or reproductive organs are most likely to be gland cells producing mucilage. Each structure represents a single large cell with a complex aperture on the external wall. In addition, a reproductive organ similar to multilocular sporangia known in brown algae of the order Ectocarpales has been found in attachment to a compression fossil of Orestovia. These data suggest similarity of the genera studied with brown algae.     

四川龙滴水自然保护区两栖爬行动物初步调查

2004年8月对四川省松潘县龙滴水自然保护区的两栖爬行动物进行了调查。结合资料记载,该保护区共有两栖动物12种,隶属于2目4科7属,其中8种属于中国特有种;爬行动物12种,隶属于1目4科9属,其中4种属于中国特有种;动物地理区系组成以东洋界的成分为主,物种多样性较丰富。     

Facies characteristic and paleoenvironmental reconstruction of the Fahliyan Formation,Lower Cretaceous,in the Kuh-e Siah area,Zagros Basin,southern Iran

The Lower Cretaceous Fahliyan Formation, part of the Khami Group, unconformably overlies the Hith Formation and is conformably overlain by the Gadvan Formation in the study area in southern Iran. The Fahliyan Formation is a reservoir rock in Zagros Basin. This formation was investigated by a detailed petrographic analysis in order to clarify the depositional facies and sedimentary environment in the Kuh-e Siah Anticline in Boushehr Province. Petrographic studies led to the recognition of 25 microfacies that were deposited in four facies belts: tidal flat, lagoon, and shoal in inner ramp and shallow open-marine in mid-ramp environment. An absence of turbidite deposits, reefal facies, and gradual facies changes indicate that the Fahliyan Formation was deposited on a carbonate ramp. Calcareous algae and benthic foraminifera are abundant in the shallow marine carbonates of the Fahliyan Formation. These skeletal grains have been studied in order to increase the understanding of their distributions in time and space. A total of ten genera belonging to different groups of calcareous algae and 16 genera of benthic foraminifera are recognized from the Fahliyan Formation at Kuh-e Siah section.     

西藏佩枯错种子植物区系研究

为了丰富西藏高原植物区系地理研究资料,深入开展该区域植被生态学研究、生物资源开发利用与保护,该研究通过野外调查、标本采集以及查阅相关的文献资料,对西藏佩枯错种子植物区系进行了统计分析。结果表明:(1)植物种类较贫乏。佩枯错共有种子植物31科77属115种,仅占西藏种子植物的18.90%、6.73%、2.17%。其中裸子植物2科2属2种,被子植物29科75属113种;被子植物中双子叶植物有23科58属91种,单子叶植物6科17属22种。(2)优势科明显,单种科占绝对优势。所有科中有5个科至少含有7种种子植物,占该研究区总种数的39.13%,是该区系的主要组分;同时科数中有11科仅含1种植物,该类科占一定优势。(3)种子植物的区系成分较复杂,划分为3个科分布区类型和10个属分布区类型,其中温带成分占绝对优势:温带分布科、属分别占总数的29.03%和77.92%,表明本区系具有明显温带性质。(4)科属特有现象不明显,没有中国特有科分布,仅有1个中国特有属分布,说明佩枯错植物区系的特有化程度极低,表现出寒旱化与高山、高原特化综合而成的高寒植物区系的年轻性。     

Crustaceans associated to macroalgae in Bajo Pepito, Isla Mujeres, Mexican Caribbean

Crustaceans associated with macroalgae were collected for one year by scuba diving in Bajo Pepito, Isla Mujeres, mexican Caribbean. A total of 148 organisms were found: three orders, 11 families, 18 genera and 19 species in nine types of associations. The order with highest abundance was Isopoda (112), followed by Amphipoda (20) and Decapoda (16).     

广西百东河自然保护区两栖爬行动物资源调查

2004年4月～2006年6月对广西百东河自然保护区两栖爬行动物资源进行了调查,结果表明:百东河自然保护区现已记录51种两栖爬行动物,其中两栖类1目5科8属17种;爬行类2目11科25属34种。对保护区两栖爬行动物区系及资源现状进行了评价。     

Genera and species of Baetidae in Japan: <Emphasis Type="Italic">Nigrobaetis,Alainites, Labiobaetis</Emphasis>, and <Emphasis Type="Italic">Tenuibaetis</Emphasis> n. stat. (Ephemeroptera)

We describe morphological characters of the genera Nigrobaetis, Alainites, Labiobaetis, and Tenuibaetis n. stat. and provide generic situations of six Japanese species: Nigrobaetis chocoratus n. comb., N. sacishimensis n. comb., Alainites atagonis, A. florens, A. yoshinensis, and Tenuibaetis pseudofrequentus. To evaluate the polarities of the morphological characters and the monophyly of Nigrobaetis, Alainites, Labiobaetis, and Tenuibaetis, character states of these four genera were compared with the genus Cloeon as an outgroup. Labiobaetis is considered to be a monophyletic group supported by a wide paraglossa. Tenuibaetis is a monophyletic group that is distinguishable from the related genera by robust setae with a medial ridge on the dorsomedian surface of the nymphal femur. We did not find any synapomorphic characters of Nigrobaetis or Alainites. Although we tentatively treat Nigrobaetis and Alainites as distinct genera, they are considered to be paraphyletic taxa.     

A Species-Specific Polymerase Chain Reaction Assay for Rapid and Sensitive Detection of <Emphasis Type="Italic">Colletotrichum</Emphasis><Emphasis Type="Italic">capsici</Emphasis>

Colletotrichum capsici is an important fungal species that causes anthracnose in many genera of plants causing severe economic losses worldwide. A primer set was designed based on the sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a conventional PCR assay. The primer set (CcapF/CcapR) amplified a single product of 394 bp with DNA extracted from 20 Mexican isolates of C. capsici. The specificity of primers was confirmed by the absence of amplified product with DNA of four other Colletotrichum species and eleven different fungal genera. This primer set is capable of amplifying only C. capsici from different contaminated tissues or fungal structures, thereby facilitating rapid diagnoses as there is no need to isolate and cultivate the fungus in order to identify it. The sensitivity of detection with this PCR method was 10 pg of genomic DNA from the pathogen. This is the first report of a C. capsici-specific primer set. It allows rapid pathogen detection and provides growers with a powerful tool for a rational selection of fungicides to control anthracnose in different crops and in the post-harvest stage.     

Frequent concomitant presence of Desulfitobacterium spp. and "Dehalococcoides" spp. in chloroethene-dechlorinating microbial communities

The presence of chloroethene dechlorination activity as well as several bacterial genera containing mainly organohalide-respiring members was investigated in 34 environmental samples from 18 different sites. Cultures inoculated with these environmental samples on tetrachloroethene and amended weekly with a seven organic electron donor mixture resulted in 11 enrichments with cis-DCE, ten with VC, and 11 with ethene as dechlorination end product, and only two where no dechlorination was observed. “Dehalococcoides” spp. and Desulfitobacterium spp. were detected in the majority of the environmental samples independently of the dechlorination end product formed. The concomitant presence of Dehalococcoides spp. and Desulfitobacterium spp. in the majority of the enrichments suggested that chloroethene dechlorination was probably the result of catalysis by at least two organohalide-respiring genera either in parallel or by stepwise catalysis. A more detailed study of one enrichment on cis-DCE suggested that in this culture Desulfitobacterium spp. as well as Dehalococcoides spp. dechlorinated cis-DCE whereas dechlorination of VC was only catalyzed by the latter.     

西藏色季拉山蕨类植物资源特征

为了摸清色季拉山蕨类植物资源的种类和资源特征,为该区蕨类开发利用提供依据。该研究通过查阅《西藏植物志》和标本,结合色季拉山多年植物调查名录,对该区野生蕨类植物进行统计分析,并对其用途进行了描述。结果显示:(1)色季拉山共有蕨类植物资源29科、57属、196种(包括变种和变型),分别占西藏蕨类植物总科、属、种的65.91%、45.24%、41.70%;优势科为鳞毛蕨科(3属/50种)、水龙骨科(9属/31种)、蹄盖蕨科(7属/22种)、中国蕨科(6属/14种),优势属为鳞毛蕨属(27种)、耳蕨属(19种)。(2)色季拉山蕨类植物的地理成分以热带类型的科和属占优势(分别占非世界分布科、属的80.00%、68.18%),具有明显的热带性质;生态分布类型以陆生为主(118种,占60.20%),其次为附生(45种,占22.96%)。(3)色季拉山共有药用蕨类植物66种,且以清热药种类最多(30种);观赏蕨类植物共有71种(其中新增加23种);食用蕨类植物共有14种,且多以嫩叶或嫩茎或根状茎为食;少数蕨类还具有指示、工业原料、饲料和绿肥等方面的作用。研究表明,色季拉山蕨类植物资源种类丰富,在西藏蕨类植物区系中占很大比例,具有很高的开发利用价值。     

Observations of the flora and vegetation of Mt. Shennungia in western Hupeh,China

Shennungia is generally known as “The highest mountain in Central China”. It is situated at latitude 31°342'N., longitude 110°35'E. in western Hupeh. The area explored is deeply cut in all sides by five V-shaped valleys, giving the landscape a steep topography. Its summit is about 3105 meters above the sea level, and the relative altitude is from 1000-2000 meters. The climate of the region is warm temperate. The differences of humidity-warmth condition between the eastern and the western flanks are quite marked. In western Hupeh and the adjacent area of Szechuan the rugged topography still preserves some tracts of natural forests at higher elevations. Our vegetational survey is confined to localities above 1500 meters. The collection of plant samples of the flora is extended to the whole mountain from the foothill to the peak. The present article deals with only a part of the results of our survey. 1. The vertical vegetation belts of Mt. Shennungia and relationships with other regions: The vegetation belts on the eastern and the western flanks of the mountain are shown in diagram 2 and 3. The comparison of the vertical vegetation zones of the Mt. Shennungia with those of the Yülungshan in N. W. Yunnan and the eastern Himalaya to the west and with those of Hwangshan and Central Japan to the east is shown in table 4, It shows that the plant communities of the Mt. Shennungia are of temperate nature, and they are more closely related to those of Hwangshan in S. Anhwei and of Central Japan than to the eastern Himalaya. 2. Floristic composition: The generic ranges of flowering plant are relatively distinct and stable. Various distributional patterns of genera are analysized. 1) Statistics of the genera in various distributional patterns: The total number of genera of flowering plants in this region are 762, belonging to the following four categories. A) tropical genera 239 (31.3%), B) temperate genera 416 (54.7%), C) endemic genera 47 (6%), and D) comsmopolitan genera 61 (8%). 2) Endemic genera: An examination of the composition of the flora in western Hupeh reveals that 47 endemic Chinese genera occur in this mountain of which 24 are monotypic genera, 20 oligotypic and 2 multitypic as shown in Table 4. The arborescent genera are nearly all deciduous. They are of temperate nature. 3) Temperate genera: There are 416 genera in wastern Hupeh. They are subdivides into the following three groups according to their distributional patterns: A) The north temperate genera: There are 159 genera belonging to 62 families in western Hupeh. B) Eastern Asian genera: There are 117 genera belonging to 69 families in western Hupeh. Among them 22 are common to the western Szechuan, adjacent regions of Yunnan and the Eastern Himalaya. The remaining 95 genera are commom to both eastern China and Japan. C) The Eastern Asian-eastern North-American genera: Of the total 762 genera known in western Hupeh, 64 are disjunctively distributed in both eastern Asia and eastern North-America. 4) The tropical genera: Of the 762 genera of the flowering plant of western Hupeh, 239 (31%) are of tropical nature. Finally, our survey shows: 1. Many of the primitive temperate genera and endemic relicts concentrate in western Hupeh and the adjacent region of Szechuan indicating that it might be one of refuges of tertiary flora. Moreover, it might also be one of the most important regions of differentiation, development and distribution of temperature flora. 2. The vegetation of this region is not only of temperate nature, but also of a transitional nature. 3. According to an analysis of the flora and a comparison of the vertical distribution of the vegetation of Yülungshan and Eastern Himalaya to the west with Hwangshan and Central Japan to the east, the floristic affinity of western Hupeh is more closely related to eastern China and Central Japan rather than to the Eastern Himalaya, and phytogeographically this region is intermediate between the Sino-Himalayan and the Sino-Japanese patterns. However, the problem of phytogeography of western Hupeh and the adjacent region of Szechuan is a complicated one requiring further study. Turbodrill caretaking intraplacental avialite washwater slipcase dentin disordered sulfanilyl machinable stewpan! Netherward pressbodies horror abscissa, keratosis frieze. 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