共查询到20条相似文献,搜索用时 15 毫秒
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Philippe Thoquet Michele Ghérardi Etienne-Pascal Journet Attila Kereszt Jean-Michel Ané Jean-Marie Prosperi Thierry Huguet 《BMC plant biology》2002,2(1):1
Background
The legume Medicago truncatula has emerged as a model plant for the molecular and genetic dissection of various plant processes involved in rhizobial, mycorrhizal and pathogenic plant-microbe interactions. Aiming to develop essential tools for such genetic approaches, we have established the first genetic map of this species. Two parental homozygous lines were selected from the cultivar Jemalong and from the Algerian natural population (DZA315) on the basis of their molecular and phenotypic polymorphism. 相似文献3.
Marcos A Gimenes Andrea A Hoshino Andrea VG Barbosa Dario A Palmieri Catalina R Lopes 《BMC plant biology》2007,7(1):9
Background
The genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species. 相似文献4.
A SNP‐based consensus genetic map for synteny‐based trait targeting in faba bean (Vicia faba L.)
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Thomas Wood Khalil Khamassi Douglas Hobbs Krystyna Gostkiewicz Mark White Hamid Khazaei Mohamed Ali Daniel Street Gérard Duc Fred L. Stoddard Fouad Maalouf Francis C. Ogbonnaya Wolfgang Link Jane Thomas Donal M O'Sullivan 《Plant biotechnology journal》2016,14(1):177-185
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Sachiko Isobe Roland K?lliker Hiroshi Hisano Shigemi Sasamoto Tshyuko Wada Irina Klimenko Kenji Okumura Satoshi Tabata 《BMC plant biology》2009,9(1):57
Background
Red clover (Trifolium pratense L.) is a major forage legume that has a strong self-incompatibility system and exhibits high genetic diversity within populations. For several crop species, integrated consensus linkage maps that combine information from multiple mapping populations have been developed. For red clover, three genetic linkage maps have been published, but the information in these existing maps has not been integrated. 相似文献6.
Divya Chandran Natasha Sharopova Kathryn A VandenBosch David F Garvin Deborah A Samac 《BMC plant biology》2008,8(1):89
Background
Aluminum (Al) toxicity is an important factor limiting crop production on acid soils. However, little is known about the mechanisms by which legumes respond to and resist Al stress. To explore the mechanisms of Al toxicity and resistance in legumes, we compared the impact of Al stress in Al-resistant and Al-sensitive lines of the model legume, Medicago truncatula Gaertn. 相似文献7.
Melanie L Hand Noel OI Cogan Timothy I Sawbridge German C Spangenberg John W Forster 《BMC plant biology》2010,10(1):94
Background
White clover (Trifolium repens L.) is an outbreeding allotetraploid species and an important forage legume in temperate grassland agriculture. Comparison of sub-genome architecture and study of nucleotide sequence diversity within allopolyploids provides insight into evolutionary divergence mechanisms, and is also necessary for the development of whole-genome sequencing strategies. This study aimed to evaluate the degree of divergence between the O and P' sub-genomes of white clover through sequencing of BAC clones containing paired homoeoloci. The microsyntenic relationships between the genomes of white clover and the model legumes Lotus japonicus and Medicago truncatula as well as Arabidopsis thaliana were also characterised. 相似文献8.
The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.) 总被引:1,自引:0,他引:1
R. K. Varshney D. J. Bertioli M. C. Moretzsohn V. Vadez L. Krishnamurthy R. Aruna S. N. Nigam B. J. Moss K. Seetha K. Ravi G. He S. J. Knapp D. A. Hoisington 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):729-739
Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. In case of peanut
or groundnut (Arachis hypogaea L.), an amphidiploid (4X) species, not a single genetic map is, however, available based on a mapping population derived
from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145
microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several
sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population.
As a result, 144 (12.6%) polymorphic markers were identified and these amplified a total of 150 loci. A total of 135 SSR loci
could be mapped into 22 linkage groups (LGs). While six LGs had only two SSR loci, the other LGs contained 3 (LG_AhXV) to
15 (LG_AhVIII) loci. As the mapping population used for developing the genetic map segregates for drought tolerance traits,
phenotyping data obtained for transpiration, transpiration efficiency, specific leaf area and SPAD chlorophyll meter reading
(SCMR) for 2 years were analyzed together with genotyping data. Although, 2–5 QTLs for each trait mentioned above were identified,
the phenotypic variation explained by these QTLs was in the range of 3.5–14.1%. In addition, alignment of two linkage groups
(LGs) (LG_AhIII and LG_AhVI) of the developed genetic map was shown with available genetic maps of AA diploid genome of groundnut
and Lotus and Medicago. The present study reports the construction of the first genetic map for cultivated groundnut and demonstrates its utility
for molecular mapping of QTLs controlling drought tolerance related traits as well as establishing relationships with diploid
AA genome of groundnut and model legume genome species. Therefore, the map should be useful for the community for a variety
of applications.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
9.
Karine Loridon Concetta Burgarella Nathalie Chantret Frédéric Martins Jérôme Gouzy Jean‐Marie Prospéri Joëlle Ronfort 《Molecular ecology resources》2013,13(1):84-95
Extensive genomic resources are available in the model legume Medicago truncatula. Here, we present the discovery and design of the first array of single‐nucleotide polymorphism (SNP) markers in M. truncatula through large‐scale Sanger resequencing of genomic fragments spanning the genome, in a diverse panel of 16 M. truncatula accessions. Both anonymous fragments and fragments targeting candidate genes for flowering phenology and symbiosis were surveyed for nucleotide variation in almost 230 kb of unique genomic regions. A set of 384 SNP markers was designed for an Illumina's GoldenGate assay, genotyped on a collection of 192 inbred lines (CC192) representing the geographical range of the species and used to survey the diversity of two natural populations. Finally, 86% of the tested SNPs were of high quality and exhibited polymorphism in the CC192 collection. Even at the population level, we detected polymorphism for more than 50% of the selected SNPs. Analysis of the allele frequency spectrum in the CC192 showed a reduced ascertainment bias, mostly limited to very rare alleles (frequency <0.01). The substantial polymorphism detected at the species and population levels, the high marker quality and the potential to survey large samples of individuals make this set of SNP markers a valuable tool to improve our understanding of the effect of demographic and selective factors that shape the natural genetic diversity within the selfing species Medicago truncatula. 相似文献
10.
Doris Herrmann Philippe Barre Sylvain Santoni Bernadette Julier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):865-876
In alfalfa (Medicago sativa), an autotetraploid forage legume, stem length is a major component of forage yield, quality and competing ability. In this
species, flowering date is not a breeding criterion. Association mapping based on a candidate gene approach has given good
results in plants, including autotetraploid species for which genetic analyses are complex. The role of a CONSTANS-LIKE gene,
identified as a candidate for stem elongation and flowering date in the model legume M. truncatula, was tested for association with the same traits in alfalfa. Four hundred genotypes from ten cultivars were evaluated for
stem height and flowering date in two locations during 4 years. They were genotyped with simple sequence repeat markers and
a low structuration was noticed. Primers were designed to amplify and sequence two regions of the alfalfa gene homologous
to CONSTANS-LIKE. Single nucleotide polymorphisms (SNPs) were detected and their allelic dose in each genotype was scored.
Linkage disequilibrium within CONSTANS-LIKE rapidly decreased as expected. Eight SNPs with a frequency above 10% were detected
over 1,010 bp (one SNP every 126 bp on average) in the 400 genotypes. This number was lower than observed in a neutral gene
(a SNP every 31 bp on average). Highly significant associations of three SNPs to flowering date and stem height were identified.
Each SNP explained up to 4.2% of the genetic variance. Thus, as in the model species, the CONSTANS-LIKE gene was shown to
be involved in flowering date and stem height in alfalfa. 相似文献
11.
Boris Rüping Antonia M Ernst Stephan B Jekat Steffen Nordzieke Anna R Reineke Boje Müller Erich Bornberg-Bauer Dirk Prüfer Gundula A Noll 《BMC plant biology》2010,10(1):219
Background
The phloem of dicotyledonous plants contains specialized P-proteins (phloem proteins) that accumulate during sieve element differentiation and remain parietally associated with the cisternae of the endoplasmic reticulum in mature sieve elements. Wounding causes P-protein filaments to accumulate at the sieve plates and block the translocation of photosynthate. Specialized, spindle-shaped P-proteins known as forisomes that undergo reversible calcium-dependent conformational changes have evolved exclusively in the Fabaceae. Recently, the molecular characterization of three genes encoding forisome components in the model legume Medicago truncatula (MtSEO1, MtSEO2 and MtSEO3; SEO = sieve element occlusion) was reported, but little is known about the molecular characteristics of P-proteins in non-Fabaceae. 相似文献12.
Almudena Castillo Gabriel Dorado Catherine Feuillet Pierre Sourdille Pilar Hernandez 《BMC plant biology》2010,10(1):266
Background
Multi-allelic microsatellite markers have become the markers of choice for the determination of genetic structure in plants. Synteny across cereals has allowed the cross-species and cross-genera transferability of SSR markers, which constitute a valuable and cost-effective tool for the genetic analysis and marker-assisted introgression of wild related species. Hordeum chilense is one of the wild relatives with a high potential for cereal breeding, due to its high crossability (both interspecies and intergenera) and polymorphism for adaptation traits. In order to analyze the genetic structure and ecogeographical adaptation of this wild species, it is necessary to increase the number of polymorphic markers currently available for the species. In this work, the possibility of using syntenic wheat SSRs as a new source of markers for this purpose has been explored. 相似文献13.
In this study we examined the genetic diversity and geographic scale of genotype distribution within the model legume species
Medicago truncatula widely distributed in pasture and marginal agricultural lands in Greece and other Mediterranean countries. Thirty one Medicago truncatula and Medicago littorialis accessions were chosen on the basis of their geographical distributions and studied using 9 polymorphic simple sequence repeats
(SSR) markers. The number of alleles per locus varied between 3 and 7. A total of 42 alleles were detected with a mean value
of 4.66 alleles per locus. Geographic origin was not related with genotypic similarity among accessions. However, there were
instances of close genetic relatedness between accessions from neighboring locations in a geographic compartment. In conclusion,
the presented data revealed extensive M. truncatula genotype dispersal in Greece pointing to the significance of preserving local genetic resources in their natural environment. 相似文献
14.
Kevei Z Seres A Kereszt A Kaló P Kiss P Tóth G Endre G Kiss GB 《Molecular genetics and genomics : MGG》2005,274(6):644-657
The increased amount of data produced by large genome sequencing projects allows scientists to carry out important syntenic
studies to a great extent. Detailed genetic maps and entirely or partially sequenced genomes are compared, and macro- and
microsyntenic relations can be determined for different species. In our study, the syntenic relationships between key legume
plants and two model plants, Arabidopsis thaliana and Populus trichocarpa were investigated. The comparison of the map position of 172 gene-based Medicago sativa markers to the organization of homologous A. thaliana genes could not identify any sign of macrosynteny between the two genomes. A 276 kb long section of chromosome 5 of the model
legume Medicago truncatula was used to investigate potential microsynteny with the other legume Lotus japonicus, as well as with Arabidopsis and Populus. Besides the overall correlation found between the legume plants, the comparison revealed several microsyntenic regions in
the two more distant plants with significant resemblance. Despite the large phylogenetic distance, clear microsyntenic regions
between Medicago and Arabidopsis or Populus were detected unraveling new intragenomic evolutionary relations in Arabidopsis.
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. 相似文献
15.
Background
Sinorhizobium meliloti and S. medicae are symbiotic nitrogen fixing bacteria in root nodules of forage legume alfalfa (Medicago sativa L.). In Morocco, alfalfa is usually grown in marginal soils of arid and semi-arid regions frequently affected by drought, extremes of temperature and soil pH, soil salinity and heavy metals, which affect biological nitrogen fixing ability of rhizobia and productivity of the host. This study examines phenotypic diversity for tolerance to the above stresses and genotypic diversity at Repetitive Extragenic Pallindromic DNA regions of Sinorhizobium nodulating alfalfa, sampled from marginal soils of arid and semi-arid regions of Morocco. 相似文献16.
17.
Zhang Y Sledge MK Bouton JH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1367-1378
Allotetraploid white clover (Trifolium repens L.), a cool-season perennial legume used extensively as forage for livestock, is an important target for marker-assisted
breeding. A genetic linkage map of white clover was constructed using simple sequence repeat (SSR) markers based on sequences
from several Trifolieae species, including white clover, red clover (T. pratense L.), Medicago truncatula (Gaertn.) and soybean (Glycine max L.). An F1 population consisting of 179 individuals, from a cross between two highly heterozygous genotypes, GA43 and Southern Regional
Virus Resistant, was used for genetic mapping. A total of 1,571 SSR markers were screened for amplification and polymorphism
using DNA from two parents and 14 F1s of the mapping population. The map consists of 415 loci amplified from 343 SSR primer pairs, including 83 from white clover,
181 from red clover, 77 from M. truncatula, and two from soybean. Linkage groups for all eight homoeologous chromosome pairs of allotetraploid white clover were detected.
Map length was estimated at 1,877 cM with 87% genome coverage. Map density was approximately 5 cM per locus. Segregation distortion
was detected in six segments of the genome (homoeologous groups A1, A2, B1, B2, C1, and D1). A comparison of map locations
of markers originating from white clover, red clover, and alfalfa (M. sativa L.) revealed putative macro-colinearity between the three Trifolieae species. This map can be used to link quantitative trait
loci with SSR markers, and accelerate the improvement of white clover by marker-assisted selection and breeding.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
18.
Background
Various species of genus Trigonella are important from medical and culinary aspect. Among these, Trigonella foenum-graecum is commonly grown as a vegetable. This anti-diabetic herb can lower blood glucose and cholesterol levels. Another species, Trigonella caerulea is used as food in the form of young seedlings. This herb is also used in cheese making. However, little is known about the genetic variation present in these species. In this report we describe the use of ISSR and RAPD markers to study genetic diversity in both, Trigonella foenum-graecum and Trigonella caerulea. 相似文献19.
The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula 总被引:2,自引:0,他引:2
Nelson MN Phan HT Ellwood SR Moolhuijzen PM Hane J Williams A O'Lone CE Fosu-Nyarko J Scobie M Cakir M Jones MG Bellgard M Ksiazkiewicz M Wolko B Barker SJ Oliver RP Cowling WA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(2):225-238
We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorised users. 相似文献
20.
Roberto Mariotti Nicolò GM Cultrera Concepcion Muñoz Díez Luciana Baldoni Andrea Rubini 《BMC plant biology》2010,10(1):211