Trichostrongylina (Nematoda) from Malagasy Muridae. III--description of a new species of Heligmonoides Baylis, 1928 (Heligmonellidae) parasitic in Mus musculus

Heligmonoides variabilis n. sp. (Heligmosomoidea, Nippostrongylinae) a parasite of Mus musculus from Madagascar is related to H. afghanus (Tenora, 1969), H. ikeharai Hasegawa, 1990 and H. josephi (Wertheim & Durette-Desset, 1976), all having the dorsal ray divided anterior to the arising of rays 8. H. ikeharai a parasite of Tokudaia muenninki (Muridae) from Japan is the most closely related species with rays 8 arising at mid-length along the dorsal ray. It is differentiated from the new species by very long spicules (almost half of body length) and by the length of the vestibule (almost one millimeter). A new definition of the genus Heligmonoides Baylis, 1928 is proposed with a dichotomic key of the species. The biogeographic distribution and the host spectrum of the genus are described.     

Trichostrongylina (Nematoda) from Malagasy muridae. II--Description of two new species of Heligmonina (Heligmonellidae) in Nesomys rufus and Eliurus tanala

Two new species of Heligmonellidae from Madagascar are described, Heligmonina madagascariensis n. sp. in Nesomys rufus and Heligmonina tanala n. sp. in Eliurus tanala. Both species belong to the Heligmonina species with a pattern of type 1-3-1 for the right lobe of the caudal bursa and 1-4 for the left lobe. In H. madagascariensis, H. dupuisi (Desset, 1964) and H. praomyos Baylis, 1928, left ray 6 arises before ray 3 from the common trunk to rays 3 to 6 while in H. tanala and the other species, it arises at the same level. H. madagascariensis is differentiated from H. dupuisi and H. praomyos by the symmetry of the branches of the dorsal ray. H. tanala is differentiated from H. malacomys Sakka & Durette-Desset, 1988, the closely related species by a different pattern of the cuticular ridges at mid-body, by the sharpness of the tips of the spicules and by the ratio of the length of the spicules on the length of the body (6.9, 8.8% versus 25-27.8%). Heligmonina chippauxi (Desset, 1964) a parasite of Oenomys hypoxanthus from the Republic of Central Africa is considered a valid species.     

Trichostrongylina (Nematoda) from Malagasy Muridae. I--Description of two new species of Heligmonellidae in Nesomys spp

Two new species of Heligmonellidae, Heligmonina wrightae n.sp. (Nippostrongylinae) and Nesomystrongylus fissicauda n.gen., n. sp. (Brevistriatinae) are described from Madagascar in Nesomys rufus and N. audeberti (Muridae). In Nesomys audeberti, the species are coparasites. Heligmonina wrightae is differentiated from all the other species of the genus, except H. malacomysi Sakka & Durette-Desset, 1988, by the ratio of the length of the spicules on the length of the body (25-27.8% versus 9.5-7%). It differs from H. malacomysi by the pattern of the caudal bursa and by the angle of the axis of orientation of the cuticular ridges on the sagittal axis. Nesomystrongylus fissicauda is related to the genus Fissicauda Durette-Desset & Krishnasamy, 1976, by the absence of the carene, by the ridges discontinuous on all the sides of the body and by the deeply divided dorsal ray. It differs from this genus by a different structure of the ridges, by the pattern of the caudal bursa, (very tiny rays 2 and strongly developed rays 3, rays 8 arising from common trunk of rays 2 to 6) and by the presence of a caudal tip in the female.     

New Pudicinae (Trichostrongylina, Heligmosomoidea), Pudica ctenomydis n. sp. parasite of Ctenomys talarum (Rodentia: Octodontidae) from Argentina

A total of 138 nematodes were found in the small intestine of Ctenomys talarum (Octodontidae) from Mar de Cobo, Argentina. A new nematode species, Pudica ctenomydis n. sp., is described. The new species more closely resembles P. pujoli Durette-Desset, 1990, parasite of Microcavia niata Thomas, from Bolivia. It can be distinguished from P. pujoli by the number of ridges and characteristics of the synlophe, the spicular morphology, differences in length between rays 9 and 10, and by the presence of a symmetrical caudal bursa and a cuticular expansion surrounding the body between vulva and anus in females.     

Description of two new species of Nippostrongylinae (Nematoda, Trichostrongyuna, Heligmosomoidea) coparasites in Arvicanthis ansorgei (Muridae) from Cameroon

In the small intestine of a single Arvicanthis ansorgei from Cameroon, two new species of Nippostrongylinae were found: Neoheligmonella zero n. sp. and Heligmonina comerounensis n. sp. N. zero belongs to the Neoheligmonella species in which the right dorsal ridge is poorly developed. Among these species, N. bainae (Durette-Desset, 1970), a parasite of Steatomys opinus from Burkina Fasso, is a closely related species. It is differentiated by the presence of vulvar alae, the ratio uterus length/body length and the separation of rays 2 and 3 at two thirds of their length. N. zero is also closely related to N. kenyae (Yeh, 1958) a parasite of Rattus rattus kijabius from Kenya by the pattern of the caudal bursa and the ratio spicules length/body length. The synlophe of N. kenyae was not described in detail but it is differentiated from N. zero by the position of the excretory pore, situated just posteriorly to the nerve ring and in the female by the ratio ovejector length/body length which is smaller. H. camerounensis n. sp. is characterised by the ratio dorsal ridges/ventral ridges which is 4/7. It is differentiated from the species of which the synlophe has not been described by the pattern of the caudal bursa (type 1-4 with tendancy 1-3-1). It is the first report of Nippostrongylinae species in Cameroon and the first record of a species of the genus Heligmonina in an Arvicanthis.     

Trichostrongylina (Nematoda) parasitic in Phyllotis sp. (Rodentia: Sigmodontinae) from Argentina, with description of three new species

The Trichostrongylina parasitic in the leaf-eared mouse Phyllotis sp. (Sigmodontinae) from the Province of Catamarca, Argentina are studied. Three new species of Stilestrongylus Freitas, Lent and Almeida, 1937 (Heligmosomoidea: Heligmonellidae: Nippostrongylinae) are described. Stilestrongylus andalgala n. sp. is distinguished from the most closely related species S. moreli Diaw, 1976 parasitic in Auliscomys boliviensis from Bolivia, by the absence of a common trunk of right rays 2 and 3, by left ray 4 shorter than ray 5, and by the shape of the female tail, without a mucron. Stilestrongylus catamarca n. sp. is distinguished from all the other species in the genus by hypertrophied rays 2, larger than rays 3, and from the closely related species Stilestrongylus barusi Durette-Desset, 1971, parasitic in Sigmodontomys alfari from Colombia, by right ray 4 longer than ray 5 and left ray 4 shorter than ray 5, by a larger spicule length/body length ratio (22.6% vs. 7.2%), and by the presence of a mucron on the female tail. Stilestrongylus gracielae n. sp. most closely resembles Stilestrongylus azarai Durette-Desset and Sutton, 1985, parasitic in Akodon azarae and in Graomys griseoflavus, and Stilestrongylus franciscanus Digiani and Durette-Desset, 2003, also parasitic in G. griseoflavus, both from Argentina. These latter differ from the new species by the right lobe of the caudal bursa: in S. azarai rays 4, 5 and 6 arise at same level from their common trunk and in S. franciscanus right rays 4 and 5 diverge at their distal extremity. Lamanema chavezi Becklund, 1963 (Molineoidea: Molineinae), a parasite of South American camelids, is reported in rodents for the second time.     

Two new species of Nippostrongylinae (Nematoda,Trichostrongylina) parasites of Cricetomys gambianus and Arvicanthis niloticus (Muridae) from Senegal

Two new Nippostrongylinae (Heligmosomoidea, Heligmonellidae) originating from Senegal are described. Heligmonina bioccai n. sp., a parasite of Cricetomys gambianus (Cricetomyinae) found in the surroundings of Dakar and Neoheligmonella bai n. sp., a parasite of Arvicanthis niloticus (Murinae) from the Province of Richard Toll. H. bioccai n. sp. is related to H. hybomysi (Durette-Desset, 1966) a parasite of Hybomys univittatus from the Central African Republic by some characteristics of the caudal bursa and of the synlophe. They are the only two species with the same pattern of caudal bursa: type 1-3-1 for the right lobe, 2-3 for the left lobe, and with the same number and disposition of the cuticular ridges at mid-body in the female. The two species are differentiated by the size (three times smaller in H. hybomysi), the ratio of spicule length on body length (7.6% versus 17% in H. hybomysi) and by a different synlophe in male and posterior part of female. N. bai n. sp. is differentiated from the closely related species N. dielmensis Diouf, Ba and Durette-Desset, 1997, also a parasite of Arvicanthis niloticus from Senegal by rays 8 arising asymmetrically on the dorsal ray and by the deirids situated at the same level as the excretory pore.     

Graphidioides subterraneus n. sp. (Nematoda: Trichostrongyloidea) from the South American subterranean rodent Ctenomys talarum Thomas, 1898 (Rodentia: Octodontidae)

A new nematode species, Graphidioides subterraneus n. sp., found in the stomach of C. talarum from Argentina is described. The new species more closely resembles G. mazzai Lent & Freitas, 1935, parasite of Galea leucoblephara from Argentina, and G. kravetzi Sutton & Durette-Desset, 1995, parasite of Holochilus brasiliensis from Uruguay. It can be distinguished by shorter spicules, by the shape of the gubernaculum, by shorter uterine branches, and by a different number of ridges of the synlophe all along the body.     

Two new species of Nippostrongylinae (Nematoda: Trichostrongylina: Heligmonellidae) from the grey leaf-eared mouse Graomys griseoflavus (Sigmodontinae) in Argentina

Two new species of Nippostrongylinae, Hassalstrongylus puntanus n. sp., and Stilestrongylus franciscanus n. sp. are described from the intestine of the grey leaf-eared mouse Graomys griseoflavus (Waterhouse, 1837) (Sigmodontinae) from the Province of San Luis, Argentina. Hassalstrongylus puntanus n. sp. is distinguished from the most closely related species H. dollfusi (Díaz-Ungría, 1963), a parasite of Mus musculus from Venezuela by longer rays 2, shorter rays 4, proximal half of the dorsal ray non-enlarged and a non-retractile female tail. Stilestrongylus franciscanus n. sp. is distinguished from the most closely related species S. flavescens Sutton & Durette-Desset, 1991, a parasite of Oryzomys flavescens from Argentina, by rays 2 and 3 diverging separately from common trunk of rays 2 to 6, and by right ray 3 arising from this trunk more distally than ray 6. Stilestrongylus azarai Durette-Desset & Sutton, 1985, a parasite of Akodon azarae from Buenos Aires, Argentina, was also found parasitizing G. griseoflavus, representing new host and locality records. Some additional morphological data for this species are also provided.     

Srivastavanema cynocephali n. sp. (Nematoda,Trichostrongylina, Heligmosomoidea), a parasite of a dermopteran from Indonesia

Srivastavanema cynocephali n. sp., a parasite of Cynocephalus variegatus (Dermoptera) from Indonesia, is described. This species is closely related to S. yapi Durette-Desset & Lim Boo Liat, 1975, in the characters of the synlophe at the mid-body and in the shape of the caudal bursa, but it is distinguished by the pattern of the synlophe in the posterior part of the body, very long, thin rays 2 and 3, a less developed genital cone, the absence of a membrane between left and right rays 6, and longer spicules. Its morphology is slightly less specialised than the four previously known species of the genus, which are all parasites of petauristines. The genus Srivastavanema (Singh, 1962) has a systematic position between the Heligmonellinae and the Brevistriatinae. Therefore, it may be a parasite of the Dermoptera which could have secondarily evolved within the Petauristinae. The Dermoptera may have played an important role in the evolution of the Heligmonellinae.     

Neoheligmonella acomysi n. sp. (Trichostrongyloidea,Nippostrongylinae), parasite d'Acomys dimidiatus Muridae de Somalie

Résumé Neoheligmonella acomysi n. sp., parasite d'Acomys dimidiatus en Somalie est proche de N. houini Durette-Desset, 1970, de N. pseudospira Durette-Desset, 1970, toutes deux parasites d'Arvicanthis abyssinicus en Ethiopie, et de N. dossoi Durette-Desset & Cassone, 1986, parasite d'Uranomys ruddi en Côte d'Ivoire. Il se sépare de ces trois espèces par de longues côtes ventrales, une côte dorsale divisée dans son tiers moyen, des spicules à pointe épaisse et un synlophe de sept et non six arêtes ventrales.

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Neoheligmonella acomysi n. sp., a parasite of Acomys dimidiatus in Somalia, is closely related to N. houini Durette-Desset, 1970 and N. pseudospira Durette-Desset, 1970, both parasites of Arvicanthis abyssinicus in Ethiopia, and to N. dossoi Durette-Desset & Cassone, 1986, a parasite of Uranomys ruddi in the Ivory Coast. It is differentiated from those species by its long ventral rays, the division of the dorsal ray in the middle third, the thickness of the tips of the spicule and a synlophe with seven ventral longitudinal cuticular ridges.

     

Helminthoxys abrocomae n. sp. (Nematoda: Oxyurida) from Abrocoma cinerea in Bolivia

A new pinworm parasite is described from Abrocoma cinerea, a caviomorph rodent of the superfamily Octodontoidea from the Andes of Bolivia. The new species, Helminthoxys abrocomae n. sp., possesses special secretory mamelons which we consider a synapomorphy of the genus Helminthoxys.Within Helminthoxys, the closest relatives are found in octodontoid rodents: H. gigantea occurs in Octodon degus in Chile and O. bridgesiin Argentina, and H. freitasi is a parasite of Thrichomys aperoides in Brazil. H. abrocomae n. sp. differs from both other species morphometrically in relation to different parts of the body in both sexes, particularly the size of the body, spicule, gubernaculum and eggs, by the presence of a rough cuticular area around the cephalic sensory papillae and by the possession of very well-developed cervical alae which are strongly curved dorsally. H. abrocomae n. sp. is the tenth nominal species described in Helminthoxys, all of them being parasites of caviomorph rodents.     

Three new species of the genus Oswaldocruzia Travassos, 1917 (Nematoda, Trichostrongylina, Molineoidea) parasites of Enyalius spp. (Iguanidae) from Brazil

Three new species of the genus Oswaldocruzia Travassos, 1917 belonging to the sub-family Molineinae are described from the stomach and/or the small intestine of Enyalius spp. from Brazil. They belong to group 6 of Ben Slimane, Chabaud & Durette-Desset (1996). In this group they share along with O. peruensis Ben Slimane, Verhaag & Durette-Desset, 1995, a parasite of Iguanidae from Peru the followings linked characters: (i) a caudal bursa of type II; (ii) cervical alae present; (iii) undulated cuticular ridges. The Peruvian species differs from the Brasilian species by the absence of a strut in the cervical alae, by a small number of cuticular ridges at mid-body and by a spicular fork with a ramified inner twig. Oswaldocruzio fredi n. sp., a parasite of the stomach and the small intestine of Enyalius iheringii, mainly differs from the two other species by the absence of the oesophageal ventral cuticular ridges. Oswaldocruzia benslimanei n. sp., a parasite of the small intestine of Enyalius bilineatus, differs from Oswoldocruzia burseyi n. sp., a parasite of the stomach of Enyalius perditus, by the division of the fork at 23.4 % of spicule length (versus 32 %), and the length of the blade longer than the fork. Oswoldocruzia subauricularis sensu Freitas, 1955 nec Rudolphi, 1819 and O. mazzai sensu Vicente, 1981 nec Travassos, 1935 should be considered as species inquirendae.     

Schulzia chiribita n. sp. (Nematoda, Trichostrongylina, Molineoidea) parasite of Leptodactylus rhodonotus (Amphibian) from Peru

A third species of the genus Schulzia Travassos, 1937 a parasite of Leptodactylus rhodonotus (Amphibian, Leptodactylidae) originating from Peru is described. By the pattern of the caudal bursa, the specimens are closely related to the two other species. They are distinguished from Schulzia uzu Lent & Santos, 1989, parasite from Atelopus oxyrhynchus in Venezuela, by the shape of the ovejector and from Schulzia travassosi Durette-Desset, Baker & Vaucher, 1985, parasite from Bufo crucifer in Brasil, Bufo granulosus and Leptodactylus bufonius in Paraguay, by the shape of the spicules. The presence of a new species in Peru points out the wide geographic distribution of the genus in the Neotropical region.     

Spalacina yanchevi n. g., n. sp. (Nematoda: Heligmonellidae) from the lesser mole rat Spalax leucodon (Rodentia: Spalacidae)

Spalacina n. g. (Heligmonellidae, Nippostrongylinae) is erected for S. yanchevi n. sp. (type-species) [syns Heligmonina nevoi of Genov & Janchev (1982) and Genov (1984)] from Spalax leucodon (Spalacidae) and two other species from spalacid rodents previously considered as members of Heligmonina Baylis, 1928: S. spalacis (Sharpilo, 1973) n. comb. and S. nevoi (Wertheim & Durette-Desset, 1975) n. comb. The new genus belongs to the subfamily Nippostrongylinae and is closely related to the genus Heligmonina from which it can be distinguished by a greater angle of rotation of the synlophe, the absence of a gradient on the ventral ridges and a weakly developed right dorsal ridge. The zoogeographical distribution of Spalacina spp. is associated with that of Palaearctic spalacids. S. yanchevi differs from S. spalacis and S. nevoi in the number and size of the ridges, the distance between the extremities of the rays 6 and 8, the degree of reduction of the dorsal ray and the length of the spicules.     

Rhabditis (Oscheius) pheropsophi n. sp. (Rhabditida: Rhabditidae)

Rhabditis (Oscheius) pheropsophi n. sp., associated with cadavers of the bombardier beetle, Pheropsophus aequinoctialis, is described from material collected in Brazil. Mean body length of the female is 1,217 μm, of the male 872 μm, and of the dauer juvenile 568 μm. The female has six lips with one papilla on each lateral lip and two on each sublateral lip; stoma wall thickened dorsally, metarhabdions with warts, excretory pore near base of esophagus, tail long (c = 8), and phasmids prominent, protruding on scanning electron microscope preparations. The male has 10 pairs of bursal ribs, with the terminal pair considerably smaller than the others; spicules fused distally two-thirds of their length. The new species can be distinguished from other members of the Dolichura-group by its fused spicules.     

Descriptions of Chitwoodius brasiliensis n.sp., Chitwoodius rusticulus n.sp., and Vancterlindia venata n.sp. (Dorylaimida: Tylencholaimidae: Vanderlindiinae)

Chitwoodius brasiliensis n.sp. is described from soil around plant roots in Vicosa, MG State, Brazil. It has a body 1.9 mm (1.6-2.5) long, odontostyle and odontophore 33 μm (31-37) and 34 μm (31-36) long, respectively, and males with spicules 59-61 μm long. Chitwoodius rusticulus n.sp. from Colombian rain forest differs from other species of the genus in having a weakly muscular anterior part of the oesophagus, a pore-like vulva, and an unsclerotized vagina. Vanderlindia venata n.sp. from lucerne soil in South Kilimanjaro, Tanzania, differs from the type and only other known species of its genus in having a smaller and less slender body (L = 3.34 mm [3.15-3.71], a = 50 [46-53]), odontostyle 2.3-2.5 times lip region width long, and stylet guiding ring located at 1.2-1.4 times lip region width from anterior end.     

Tetrameres (Gynaecophila) aspicula n. sp. (Nematoda: Tetrameridae), a proventricular parasite of the white-faced ibis Plegadis chihi in Argentina

Tetrameres (Gynaecophila) aspicula n. sp. is described from the proventriculus of the white-faced ibis Plegadis chihi (Vieillot) (Ciconiiformes, Threskiornithidae) from Argentina. The new species is characterised by the absence of spicules, by possessing two ventral rows of extremely small spines in males, extending along the second half of body length, and by the tiny, very feebly developed postcloacal papillae. T. (G.) aspicula n. sp. is compared to the remainder of the species in the subgenus as well as to other species of Tetrameres which lack or possess feebly developed spines. The absence of spicules is a character shared with two other species in the genus, T. (G.) gynaecophila and T. (G.) deccani,from which the new species differs in body size, the arrangement of caudal papillae and the somatic spination in males. A pair of somatic papillae, previously unreported in species of this genus, was found just on or ventral to the lateral line at various regions of the body length. The homology of these structures to other paired somatic papillae described in nematodes is discussed.     

Heterorhabditis atacamensis n. sp. (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Atacama Desert, Chile

A new Heterorhabditis species of entomopathogenic nematode was isolated from soil of the Atacama Desert in Chile. The new species is characterized by morphometrics of the infective juvenile (IJ) with length (L)?=?611 (578-666)?μm, head to excretory pore length (EP)?=?115 (101-126)?μm, tail?=?69 (62-79)?μm long, (EP/tail)?×?100 (E%)?=?165 (149-182) and L/maximum body diameter (ratio a)?=?28 (25-31). The male has spicules 45 (40-49)?μm long, gubernaculum 20 (17-22)?μm long and (spicule length/anal body diameter)?×?100 (SW%)?=?205 (179-249). The hermaphroditic adult has shallow cuticular folds immediately anterior and posterior to the vulva, a slight post-anal swelling and a finely rounded tail terminus. Morphologically, H. atacamensis n. sp. resembles H. safricana, H. marelatus, H. downesi and H. amazonensis, but can be distinguished by characters of adult and IJ stages. In particular, for adult males, H. atacamensis n. sp. differs from H. amazonensis by the number and orientation of the genital papillae and from H. downesi by the position of the excretory pore; by the shape of the female tail terminus from H. downesi and by the position of the IJ hemizonid from H. marelatus. Heterorhabditis atacamensis n. sp. is further characterized by internal transcribed spacer (ITS) and D2D3 rDNA sequences, the closest species, H. safricana, being separated by 13?bp across 730?bp of the ITS (incorporating ITS1 (partial sequence), 5.8S (complete sequence), ITS2 (complete sequence)) and 5?bp across 592?bp of the partial 28S (incorporating D2D3) sequence. The morphological and molecular data confirm that H. atacamensis n. sp. is a valid species.     

Description of a new species of Heligmonina Baylis, 1928 (Nematoda: Heligmonellidae) a parasite of Mastomys natalensis (Rodentia: Muridae) from Swaziland and new data on the synlophe of Heligmonina chabaudi (Desset, 1966)

A new species of heligmosomoid nematode belonging to the subfamily Nippostrongylinae Durette-Desset, 1970 is described: Heligmonina wakelini n. sp., a parasite from the small intestine of the commensal rodent Mastomys natalensis (Smith, 1834) from Swaziland. It differs from the most closely related species H. boomkeri Durette-Desset & Digiani, 2005 by the number of the cuticular ridges in the female synlophe (10 vs 12), the width of the left ala, larger than the body diameter in the male, and the inclination of the axis of orientation of the ridges in both sexes (53 degrees vs 70 degrees). New morphological data (head and synlophe) on Heligmonina chabaudi (Desset, 1964), also a parasite of Mastomys natalensis in the Republic of Congo, are provided in order to compare with the new species.