Assessing release protocols for Canada lynx reintroduction in colorado

Reintroductions are a common strategy to restore ecosystem integrity, especially when top predators are involved. Reintroductions are often time consuming, expensive, and controversial, and thus understanding what aspects are important for a successful program is needed. Focusing on the example of the reintroduction of Canada lynx (Lynx canadensis) to Colorado, we investigated how different release protocols (RP) affected mortality within the first year post-release. We found that average monthly mortality in the study area during the first year decreased with time in captivity from 0.205 (95% CI = 0.069, 0.475) for lynx having spent up to 7 days in captivity to 0.028 (95% CI = 0.012, 0.064) for lynx spending >45 days in captivity before release. Our results also suggest that keeping lynx in captivity beyond 5–6 weeks accrued little benefit in terms of monthly survival. We found that, on a monthly average basis, lynx were as likely to move out (P = 0.196, SE = 0.032) as well as back onto (P = 0.143, SE = 0.034) the reintroduction area during the first year after release. Mortality was 1.6 times greater outside of the study area, suggesting that permanent emigration and differential mortality rates on and off reintroduction areas should be factored into sample size calculations for an effective reintroduction effort. A post-release monitoring plan is critical to providing information to assess aspects of RP and to improve survival of individuals. Future lynx and other carnivore reintroductions may use our results to help design reintroduction programs including both the release and post-release monitoring protocols. © 2011 The Wildlife Society.     

Competitive PCR-ELISA protocols for the quantitative and the standardized detection of viral genomes

Competitive PCR-ELISA combines competitive PCR with an ELISA to allow quantitative detection of PCR products. It is based on the inclusion of an internal standard competitor molecule that is designed to differ from the target by a short sequence of nucleotides. Once such a competitor molecule has been designed and constructed, target and competitor sequences are concurrently PCR-amplified, before hybridization to two different specific probes and determination of their respective OD values by ELISA. The target can be quantified in relation to a titration curve of different dilutions of the competitor. The competitor can alternatively be used at a unique optimal concentration to allow for standardized detection of the target sequence. PCR-ELISA can be performed in 1 d in laboratories without access to a real-time PCR thermocycler. This technique is applied in diagnostics to monitor the course of infections and drug efficacy. Competitive PCR-ELISA protocols for the quantitative and for the standardized detection of parvovirus B19 are detailed here as an example of the technique.     

Gauging megadiversity with optimized and standardized sampling protocols: A case for tropical forest spiders

Characterizing and monitoring biodiversity and assessing its drivers require accurate and comparable data on species assemblages, which, in turn, should rely on efficient and standardized field collection. Unfortunately, protocols that follow such criteria remain scarce and it is unclear whether they can be applied to megadiverse communities, whose study can be particularly challenging. Here, we develop and evaluate the first optimized and standardized sampling protocol for megadiverse communities, using tropical forest spiders as a model taxon. We designed the protocol COBRA‐TF (Conservation Oriented Biodiversity Rapid Assessment for Tropical Forests) using a large dataset of semiquantitative field data from different continents. This protocol combines samples of different collecting methods to obtain as many species as possible with minimum effort (optimized) and widest applicability and comparability (standardized). We ran sampling simulations to assess the efficiency of COBRA‐TF (optimized, non‐site‐specific) and its reliability for estimating taxonomic, phylogenetic, and functional diversity, and community structure by comparing it with (1) commonly used expert‐based ad hoc protocols (nonoptimized, site‐specific) and (2) optimal protocols (optimized, site‐specific). We then tested the performance and feasibility of COBRA‐TF in the field. COBRA‐TF yielded similar results as ad hoc protocols for species (observed and estimated) and family richness, phylogenetic and functional diversity, and species abundance distribution. Optimal protocols detected more species than COBRA‐TF. Data from the field test showed high sampling completeness and yielded low numbers of singletons and doubletons. Optimized and standardized protocols can be as effective in sampling and studying megadiverse communities as traditional sampling, while allowing data comparison. Although our target taxa are spiders, COBRA‐TF can be modified to apply to any highly diverse taxon and habitat as long as multiple collecting techniques exist and the unit effort per sample is comparable. Protocols such as COBRA‐TF facilitate studying megadiverse communities and therefore may become essential tools for monitoring community changes in space and time, assessing the effects of disturbances and selecting conservation areas.     

Undergraduate and postgraduate medical education in Canada

An overview of medical education at both the undergraduate and postgraduate levels in Canadian faculties of medicine is provided. Particular attention is focused on changes that have occurred in the 1990s and their effect on medical students and on educational programs. Also considered are the effects of reductions in the number of entry-level positions for residency training and the changes in educational requirements for licensure on senior medical students.     

Bioassessment of benthic macroinvertebrates in wetlands: a paired comparison of two standardized sampling protocols

Wetlands Ecology and Management - We compared a rapid bioassessment protocol (Traveling Sweep Approach [TSA]) with a more conventional time intensive protocol (Composite Transect Approach [CTA]) to...     

Time lags in ecological systems.

A model representing ten species in a four trophic level community is constructed by using Volterra's equations including time lags, and is solved numerically for some values of the parameters. Classical discrete and continuous time lags yield similar results; simulating with discrete time lags thus appears useful. Parasitic versus predatory structures are compared by measuring the amplitudes of oscillations and the time taken to settle down to equilibrium, and give the following qualitative conclusions for the model.An increase in the carrying capacity of a trophic level increases the destabilizing influence of time lag in that level, this increase is more marked in predatory structures. Time lags appear to cause more violent oscillations in species strongly linked to a predatory system than in weakly linked species. The oscillatory period increases in predatory systems as the time lag is moved to higher levels, while it decreases in parasitic systems.     

Population growth lags in introduced species

When introduced to new ecosystems, species'' populations often grow immediately postrelease. Some introduced species, however, maintain a low population size for years or decades before sudden, rapid population growth is observed. Because exponential population growth always starts slowly, it can be difficult to distinguish species experiencing the early phases of slow exponential population growth (inherent lags) from those with actively delayed growth rates (prolonged lags). Introduced ungulates provide an excellent system in which to examine lags, because some introduced ungulate populations have demonstrated rapid population growth immediately postintroduction, while others have not. Using studies from the literature, we investigated which exotic ungulate species and populations (n = 36) showed prolonged population growth lags by comparing the doubling time of real ungulate populations to those predicted from exponential growth models for theoretical populations. Having identified the specific populations that displayed prolonged lags, we examined the impacts of several environmental and biological variables likely to influence the length of lag period. We found that seventeen populations (47%) showed significant prolonged population growth lags. We could not, however, determine the specific factors that contributed to the length of these lag phases, suggesting that these ungulate populations'' growth is idiosyncratic and difficult to predict. Introduced species that exhibit delayed growth should be closely monitored by managers, who must be proactive in controlling their growth to minimize the impact such populations may have on their environment.