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A nuclear‐encoded chloroplast‐targeted S1 RNA‐binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana
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Ji Hoon Han Kwanuk Lee Kwang Ho Lee Sunyo Jung Young Jeon Hyun‐Sook Pai Hunseung Kang 《The Plant journal : for cell and molecular biology》2015,83(2):277-289
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Bisanz C Bégot L Carol P Perez P Bligny M Pesey H Gallois JL Lerbs-Mache S Mache R 《Plant molecular biology》2003,51(5):651-663
Altered pigmentation is an easily scored and sensitive monitor of plastid function. We analyzed in detail a yellow colored transposon-tagged mutant (dal1-2) that is allelic to the dal mutant previously identified (Babiychuk et al., 1997). Mesophyll cells of mutant plants possess abnormal nucleoids and more but smaller plastids than wild type cells. Plastid development in dal1-2 is not altered in the dark but is arrested at the early steps of thylakoid assembly. The amino acid sequence of the protein deduced from our cDNA clone is 21 amino acids longer than the previously published DAL sequence (Babiychuk et al., 1997) and allowed us to show that DAL codes for a chloroplast protein. The dal1-2 mutation has a global negative effect on plastid RNA accumulation and on expression of nuclear encoded photosynthetic genes. We show that the plastid RNA polymerases, the nuclear-encoded NEP and the plastid-encoded PEP, are functional in the mutant. Precursor 16S and 23S rRNA species specifically accumulate at a high level in the mutant but the 5-end and the long 3-end trailer are not modified. We suggest that the dal mutation is involved in plastid rRNA processing and consequently in translation and early chloroplast differentiation. 相似文献
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Michel Goldschmidt-Clermont Jacqueline Girard-Bascou Yves Choquet Jean-David Rochaix 《Molecular & general genetics : MGG》1990,223(3):417-425
Summary In Chlamydomonas reinhardtii the three exons of the psaA gene are widely scattered on the chloroplast genome: exons 1 and 2 are in opposite orientations and distant from each other and from exon 3. The mature mRNA, encoding a core polypeptide of photosystem I, is thus probably assembled from separate precursors by splicing in trans. We have isolated and characterized a set of mutants that are deficient in the maturation of psaA mRNA. The mutants belong to 14 nuclear complementation groups and one chloroplast locus that are required for the assembly of psaA mRNA. The chloroplast locus, tscA, is remote from any of the exons and must encode a factor required in trans. The mutants all show one of only three phenotypes that correspond to defects in one or other or both of the joining reactions. These phenotypes, and those of double mutants, are consistent with the existence of two alternative splicing pathways. 相似文献
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The three genomes of Chlamydomonas 总被引:1,自引:0,他引:1
Rochaix JD 《Photosynthesis research》2002,73(1-3):285-293
During the past 50 years, the green unicellular alga Chlamydomonas reinhardtii has played a key role as model system for the study of photosynthesis and chloroplast biogenesis. This is due to its well-established
nuclear and chloroplast genetics, its dispensable photosynthetic function in the presence of acetate, and its highly efficient
nuclear and chloroplast transformation systems. Considerable progress has been achieved in our understanding of the structure,
function, inheritance, and expression of nuclear, chloroplast, and mitochondrial genes and of the molecular cross-talk between
the nuclear, chloroplast, and mitochondrial genetic systems.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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The sequence and expression of mRNA homologous to a cDNA encoding a non-photosynthetic ferredoxin (Fd1) from Citrus fruit was investigated. The non-photosynthetic nature of this ferredoxin was deduced from: (1) amino acid sequence alignments showing better scores with non-photosynthetic than with photosynthetic ferredoxins, (2) higher expression in tissues containing plastids other than chloroplast such as petals, young fruits, roots and peel of fully coloured fruits, and (3) the absence of light-dark regulation characteristic of photosynthetic ferredoxins. In a phylogenetic tree constructed with higher-plant ferredoxins, Citrus fruit ferredoxin clustered together with root ferredoxins and separated from the photosynthetic ferredoxins. Non photosynthetic (root and fruit) ferredoxins, but not the photosynthetic ferredoxins, have their closest homologs in cyanobacteria. Analysis of ferredoxin genomic organization suggested that non-photosynthetic ferredoxins exist in Citrus as a small gene family. Expression of Fd1 is developmentally regulated during flower opening and fruit maturation, both processes may be mediated by ethylene in Citrus. Exogenous ethylene application also induced the expression of Fd1 both in flavedo and leaves. The induction of non-photosynthetic ferredoxins could be related with the demand for reducing power in non-green, but biosynthetically active, tissues. 相似文献