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1.
M. Das  Govindjee 《BBA》1967,143(3):570-576
When Chlorella cells are ruptured at pH 4.6 by sonication in air, its absorption spectrum can be best explained if one assumes that a long-wave chlorophyll a form (Chl a 693) is preferentially destroyed. Using these preparations, and comparing them with the algal suspension and the sonicates prepared at pH 7.8 under argon, we make the following conclusions: (a) The red drop beginning at about 675–680 nm in the action spectrum* of fluorescence at 298 °K must be due to the presence of a non-(or weakly) fluorescent form of chlorophyll a. We suggest that this form is Chl a 693. The red drop is absent in the aerobic sonicates. (b) The red drop in fluorescence in whole algal cells is not due to any errors in absorption measurements; this drop is clearly present in the anaerobic sonicates. (c) The emission band at 723 nm, discovered by in whole Chlorella cells at 77 °K, may be due to increased fluorescence efficiency of Chl a 693 at low temperature; the F723 band is absent in aerobic sonicates.  相似文献   

2.
To determine the fluorescence properties of cyanobacterial Photosystem I (PS I) in relatively intact systems, fluorescence emission from 20 to 295 K and polarization at 77 K have been measured from phycobilisomes-less thylakoids of Synechocystis sp. PCC 6803 and a mutant strain lacking Photosystem II (PS II). At 295 K, the fluorescence maxima are 686 nm in the wild type from PS I and PS II and at 688 nm from PS I in the mutant. This emission is characteristic of bulk antenna chlorophylls (Chls). The 690-nm fluorescence component of PS I is temperature independent. For wild-type and mutant, 725-nm fluorescence increases by a factor of at least 40 from 295 to 20 K. We model this temperature dependence assuming a small number of Chls within PS I, emitting at 725 nm, with an energy level below that of the reaction center, P700. Their excitation transfer rate to P700 decreases with decreasing temperature increasing the yield of 725-nm fluorescence.Fluorescence excitation spectra of polarized emission from low-energy Chls were measured at 77 and 295 K on the mutant lacking PS II. At excitation wavelengths longer than 715 nm, 760-nm emission is highly polarized indicating either direct excitation of the emitting Chls with no participation in excitation transfer or total alignment of the chromophores. Fluorescence at 760 nm is unpolarized for excitation wavelengths shorter than 690 nm, inferring excitation transfer between Chls before 760-nm fluorescence occurs.Our measurements illustrate that: 1) a single group of low-energy Chls (F725) of the core-like PS I complex in cyanobacteria shows a strongly temperature-dependent fluorescence and, when directly excited, nearly complete fluorescence polarization, 2) these properties are not the result of detergent-induced artifacts as we are examining intact PS I within the thylakoid membrane of S. 6803, and 3) the activation energy for excitation transfer from F725 Chls to P700 is less than that of F735 Chls in green plants; F725 Chls may act as a sink to locate excitations near P700 in PS I.Abbreviations Chl chlorophyll - BChl bacteriochlorophyll - PS Photosystem - S. 6803 Synechocystis sp. PCC 6803 - PGP potassium glycerol phosphate  相似文献   

3.
Anabaena variabilis cells have been cultivated in the presence of diphenylamine (12 mg/l) which inhibits the biosynthesis of β-carotene, echinenone and zeasanthin. The content of chlorophyll a is also reduced by diphenylamine. The biosynthesis of myxoxanthophyll is, however, stimulated by this reagent.

The membrane fragments prepared from Anabaena cells grown in the presence of diphenylamine have the activities of both Photosystem 1 (NADP+ reduction with DCIP-ascorbate as electron donor) and Photosystem 2 (DCIP reduction with 1,5-diphenylcarbazide as electron donor).

The fluroescence spectra of these cells at 77°K show peaks at 696 and 731 nm and a shoulder around 687 nm. The fluorescence intensity at 687 and 696 nm is higher in these cells than in normal-Anabaena cells.  相似文献   


4.
A chlorophyll type pigment (F698) fluorescing maximally at 698 mμ at 77°K has been observed in preparations of chlorophyll. This fluorescence is quenched by small amounts of naturally occurring materials, including plastoquinone and the ubiquinones, and by nitrobenzene, probably by formation of a nonfluorescent complex. Fluorescence quenching does not occur in the presence of carotenes, xanthophylls, or reduced plastoquinone and ubiquinone. The fluorescence is sharply temperature dependent, with a steep rise in intensity occurring at 165°K. At 77°K the fluorescence yield is between 0.8 and 1.0. The red absorption maximum of the pigment is at 675 mμ at room temperature and at 688 mμ at 77°K. In vivo, a low temperature emission is also observed at 698 mμ, and this fluorescence is quenched by nitrobenzene. It is proposed that the pigment found in vitro is also the one responsible for emission at 698 mμ in vivo. A reaction of F698 with plastoquinone is suggested as the primary photochemical step in system II of photosynthesis.  相似文献   

5.
J. Amesz  M.P.J. Pulles  B.R. Velthuys 《BBA》1973,325(3):472-482

1. 1. Spinach chloroplasts were stored in the dark for at least 1 h, rapidly cooled to −40 °C, and illuminated with continuous light or short saturating flashes. In agreement with the measurements of Joliot and Joliot, chloroplasts that had been preilluminated with one or two flashes just before cooling showed a less efficient increase in the yield of chlorophyll a fluorescence upon illumination at −40 °C than dark-adapted chloroplasts. The effect disappeared below −150 °C, but reappeared again upon warming to −40 °C. Little effect was seen at room temperature in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), added after the preillumination.

2. 2. Light-induced absorbance difference spectra at −40 °C in the region 500–560 nm indicated the participation of two components, the socalled 518-nm change (P518) and C-550. After preillumination with two flashes the absorbance change at 518 nm was smaller, and almost no C-550 was observed. After four flashes, the bands of C-550 were clearly visible again.

3. 3. The fluorescence increase and the absorbance change at 518 nm showed the same type of flash pattern with a minimum after the second and a maximum at the fourth flash. In the presence of 100 μM hydroxylamine, the fluorescence response was low after the fourth and high again after the sixth flash, which confirmed the hypothesis that the flash effect was related to the so-called S-state of the electron transport pathway from water to Photosystem 2.

4. 4. The kinetics of the light-induced absorbance changes were the same at each wavelength, and, apart from the size of the deflection, they were independent of preillumination. Flash experiments indicated that the absorbance changes were a one-quantum reaction. This was also true for the fluorescence increase in dark-adapted chloroplasts, but with preilluminated chloroplasts several flashes were needed to approximately saturate the fluorescence yield.

5. 5. The results are discussed in terms of a mechanism involving two electron donors and two electron acceptors for System 2 of photosynthesis.

Abbreviations: DCMU, 3-(3,4-dichlorophenyl)-1, 1-dimethylurea  相似文献   


6.
Deprivation of iron from the growth medium results in physiological as well as structural changes in the unicellular cyanobacterium Anacystis nidulans R2. Important among these changes are alterations in the composition and function of the photosynthetic membranes. Room-temperature absorption spectra of iron-starved cyanobacterial cells show a chlorophyll absorption peak at 672 nanometers, 7 nanometers blue-shifted from its normal position at 679 nanometers. Iron-starved cells have decreased amounts of chlorophyll and phycobilins. Their fluorescence spectra (77K) have one prominent chlorophyll emission peak at 684 nanometers as compared to three peaks at 687, 696, and 717 nanometers from normal cells. Chlorophyll-protein analysis of iron-deprived cells indicated the absence of high molecular weight bands. Addition of iron to iron-starved cells induced a restoration process in which new components were initially synthesized and integrated into preexisting membranes; at later times, new membranes were assembled and cell division commenced. Synthesis of chlorophyll and phycocyanins started almost immediately after the addition of iron. The absorption peak slowly returned to its normal wavelength within 24 to 28 hours. The fluorescence emission spectrum at 77K changed over a period of 14 to 24 hours during which the 696- and 717-nanometer peaks grew to their normal levels, and the 684 nanometer peak moved to 687 nanometers and its relative intensity decreased to its normal level. Analysis of chlorophyll-protein complexes on polyacrylamide gels showed that high molecular weight chlorophyll-protein bands were formed during this time, and that low molecular weight bands (related to photosystem II) disappeared. The origin of the fluorescence emission at 687 and 696 nanometers is discussed in relation to the specific chlorophyll-protein complexes formed during iron reconstitution.  相似文献   

7.
Ted Mar  Gabriel Gingras 《BBA》1976,440(3):609-621
A randomly oriented sample of photoreaction center prepared from Rhodospirillum rubrum was excited at 77 °K by an actinic linearly polarized light of 870 nm. Under such conditions, only those chromophores with components of their absorption dipoles oriented parallel to the polarization of the actinic light are bleached. The change in absorbance at 900 nm of this photoselected sample was observed while varying the angle of polarization of a weak measuring light. The polarization of the absorbance change was thus evaluated as 0.25.

This value is interpreted to mean that P870 is attributable to two absorption dipoles forming an angle included between 35.75° and 90°. Comparison with the p value of 0.5 obtained on a similar preparation by polarization of fluorescence (Ebrey, T. G. and Clayton, R. K. (1969) Photochem. Photobiol. 10, 109–117) leads to the conclusion that either these two dipoles emit fluorescence without being coupled by singlet-singlet energy transfer or that only one of them is a fluorescence emitter in the absence of reversible singlet-singlet energy transfer.  相似文献   


8.
A new room-temperature molten salt, 1:2 LiCl-ethylaluminum dichloride (LiCl-EtAlCl2, f.p. about 178 K), is examined using 13C relaxation methods at 7.05 T (−25 to + 80 °C). The methylene carbon undergoes scalar relaxation of the ‘second kind’ as it is coupled to a faster relaxing (quadrupolar) nucleus. LiCl-EtAlCl2 undergoes a significant liquid-state phase change between 5 and 15 °C as evidenced by observed changes in the relaxation properties of the methylene and methyl carbons and J(13C−27Al). The J(13C−27Al) coupling constants are 75 (− 10 to + 5 °C) and 11 Hz (15–65 °C), indicating a change in structure between 5 and 15 °C. Chemical shift anisotropies of 56 and 48 ppm are obtained for the methylene and methyl carbons in the EtAlCl2 dimer part of the 1:2 LiCl-EtAlCl2 solution.  相似文献   

9.
对螺旋藻(Spirulinaplatensis)藻胆体在室温和77K处于不同浓度磷缓冲溶液和不同解离时间的荧光发射光谱进行了研究。藻胆体在0.9mol/L磷酸缓冲溶液中,由于没有发生解离,光能传递效率高,在77K荧光发射光谱中只有一个峰,位于687nm,属于别藻蓝蛋白-B。当藻胆体悬浮在0.3mol/L磷酸缓冲溶液中1分钟,77K荧光光谱的主峰出现在684nm.又出现655nm和666nm荧光峰,它们依次属子C-藻蓝蛋白和别藻蓝蛋白。在2小时;655nm荧先峰成为主峰,684nm荧光峰为次峰,666nm荧光肩消失。这表明C-藻蓝蛋白所捕获的先能已不能传递给别藻蓝蛋白,但能传给别藻蓝蛋白-B。我们提出在螺旋藻藻胆体中存在两类C-藻蓝蛋白,一是与别藻蓝蛋白相连接,另一是与别藻蓝蛋白-B相连接。  相似文献   

10.
对螺旋藻(Spirulinaplatensis)藻胆体在室温和77K处于不同浓度磷缓冲溶液和不同解离时间的荧光发射光谱进行了研究。藻胆体在0.9mol/L磷酸缓冲溶液中,由于没有发生解离,光能传递效率高,在77K荧光发射光谱中只有一个峰,位于687nm,属于别藻蓝蛋白-B。当藻胆体悬浮在0.3mol/L磷酸缓冲溶液中1分钟,77K荧光光谱的主峰出现在684nm.又出现655nm和666nm荧光峰,它们依次属子C-藻蓝蛋白和别藻蓝蛋白。在2小时;655nm荧先峰成为主峰,684nm荧光峰为次峰,666nm荧光肩消失。这表明C-藻蓝蛋白所捕获的先能已不能传递给别藻蓝蛋白,但能传给别藻蓝蛋白-B。我们提出在螺旋藻藻胆体中存在两类C-藻蓝蛋白,一是与别藻蓝蛋白相连接,另一是与别藻蓝蛋白-B相连接。  相似文献   

11.
Two wheat (Triticum aestivum L.) cultivars contrasting in architectonics and differing in drought resistance, Azamatli-95 (short stature, vertically oriented small leaves, drought-tolerant) and Giymatli-2/17 (short stature, broad and drooping leaves, drought-sensitive), were studied. It was found that the content of CP I (115 kDa) and 63-kDa apoprotein P700 and also LHC II polypeptides increases slightly in the drought-resistant cv. Azamatli-95 under extreme water supply limitation, while their content decreases in drought-sensitive cv. Giymatli-2/17. The intensity of synthesis of α- and β-subunits of CF1 (55 and 53.5 kDa) and 33–30.5 kDa proteins also decreases in the sensitive genotype. The intensity of short wavelength peaks at 687 and 695 nm sharply increases in the fluorescence spectra (77K) of chloroplasts from Giymatli-2/17 under water deficiency, and there is a stimulation of the ratio of fluorescence band intensity F687/F740. After exposure to drought, cv. Giymatli-2/17 shows a larger reduction in the actual PS II photochemical efficiency of chloroplasts than cv. Azamatli-95. Published in Russian in Biokhimiya, 2009, Vol. 74, No. 8, pp. 1109–1116.  相似文献   

12.
P.Muir Wood 《BBA》1974,357(3):370-379
The rate of electron transfer between reduced cytochrome ƒ and plastocyanin (both purified from parsley) has been measured as k = 3.6 · 107 M−1 · s−1, at 298 °K and pH 7.0, with activation parameters ΔH = 44 kJ · mole−1 and ΔS = +46 J · mole−1 · °K−1. Replacement of cytochrome ƒ with red algal cytochrome c-553, Pseudomonas cytochrome c-551 and mammalian cytochrome c gave rates at least 30 times slower: k = 5 · 105, 7.5 · 105 and 1.0 · 106 M−1 · s−1, respectively.

Similar measurements made with azurin instead of plastocyanin gave k = 6 · 106 and approx. 2 · 107 M−1 · s−1 for reaction of reduced azurin with cytochrome ƒ and algal cytochrome respectively.

Rate constants of 115 and 80 M−1 · s−1 were found for reduction of plastocyanin by ascorbate and hydroquinone at 298 °K and pH 7.0. The rate constants for the oxidation of plastocyanin, cytochrome ƒ, Pseudomonas cytochrome c-551 and red algal cytochrome c-553 by ferricyanide were found to be between 3 · 104 and 8 · 104 M−1 · s−1.

The results are discussed in relation to photosynthetic electron transport.  相似文献   


13.
In this paper a number of experiments with the purple bacteria Rhodospirillum rubrum and Rhodopseudomonas capsulata is described in which the total fluorescence yield and/or the total fraction of reaction centers closed after a picosecond laser pulse were measured as a function of the pulse intensity. The conditions were such that the reaction centers were either all in the open or all in the closed state before the pulse arrived. These experiments are analysed using the theoretical formalism discussed in the preceding paper (Den Hollander, W.T.F., Bakker J.G.C., and Van Grondelle, R., Biochim. Biophys. Acta 725, 492–507). From the experimental results the number of connected photosynthetic units, λ, the rate of energy transfer between neighboring antenna molecules, kh, and the rate of trapping by an open reaction center, kot, can be estimated. For R. rubrum it is found that λ = 14−17, kh = (1−2)·1012 s−1 and kot = (4−6)·1011 s−1, for Rps. capsulata λ ≈ 30, kh ≈ 4·1011 s−1 and kot ≈ 3·1011 s−1. The findings are discussed in terms of current models for the structure of the antenna and the kinetic properties of the decay processes occurring in these purple bacteria.  相似文献   

14.
The iron(II) compound of formula [Fe(bpym)(NCS)2]n (bpym = 2,2′-bipyrimidine) has been synthesized and its crystal structure determined by X-ray diffraction methods. It crystallizes in the tetragonal P41 (No. 76) and P43 space groups, a = 8.849(2), C=16.486(3) Å, V=1290.9(5) Å3, Z=4, Dc=1.699 g cm−3, Mr=330.2, F(000)=664, λ(Mo K)=0.71073 Å, μ(Mo K)=14.8 cm−1 and T=295 K. A total of 2449 reflections was collected over the range 3≤2≤55°; of these, 1657 were unique and 1321 were considered as observed (13σ(I)) and used in the structural analysis. The final R and Rw residuals were 0.027 and 0.026, respectively. The structure is made up of chiral (Δ and Λ enantiomers crystallize in the same crop) chains of iron(II) atoms bridged by bis-chelating bpym, the electroneutrality being achieved by N-bonded thiocyanato groups in cis position. Each metal atom is in a distorted FeN6 octahedral environment, the Fe---N bonds ranging from 2.265(3) to 2.028(4) . The intrachain metal-metal separation is 5.960(1) Å. Variable-temperature magnetic susceptibility data in the temperatyre range 290–4.2 K show that the iron(II) is high-spin and interacts in an antiferromagnetic fashion, the relevant parameters being . The magnitude of the exchange coupling compares well with that reported for other structurally characterized bpym-bridged iron(II) complexes.  相似文献   

15.
Studies on the optimal cooling rate for freezing human diploid fibroblasts   总被引:2,自引:0,他引:2  
Ampoules containing each 1 ml of Dulbecco's Modified Eagle Medium with 16.6% fetal calf serum and 10% dimethylsulfoxide were insulated in various ways and placed into different cooling boxes. The resulting cooling velocities of the medium ranged from about 0.7 to 102 °C/min. As revealed by cellular attachment in recovery cultures, human diploid fibroblasts cooled at about 1.5 to 4.5 °C/min to − 78 °C prior to storage in liquid nitrogen showed an optimal survival of about 60%. Survival was about 25% at cooling rates of 0.7 and 19 °C/min, respectively. The optimal cooling rate was achieved by insulating the freezing ampoules with 1 to 3 closed vessels and placing them into a dry ice chest, or into a dry ice/ethanol bath.  相似文献   

16.
Bacon Ke  Helmut Beinert 《BBA》1973,305(3):689-693
The EPR spectrum of Photosystem-I subchloroplast particles, which had been pre-illuminated in the presence of methyl viologen, showed a large P700+ signal whereas bound iron-sulfur proteins were not detected. This observation is consistent with a “one-way” electron discharge by the primary electron acceptor, P430, subsequent to the primary photochemical charge separation, and an accumulation of photooxidized P700+. Subsequent illumination of the same sample at 77 °K did not change the EPR spectrum. However, if the pre-illuminated subchloroplast particles were allowed to recover at room temperature by standing in the dark for 10 min or by addition of a chemical reductant, subsequent illumination of the sample at 77 °K yielded an EPR spectrum consisting of signals due to both P700+ and reduced iron-sulfur protein.  相似文献   

17.
Some mosses are extremely tolerant of drought stress. Their high drought tolerance relies on their ability to effectively dissipate absorbed light energy to heat under dry conditions. The energy dissipation mechanism in a drought-tolerant moss, Bryum argenteum, has been investigated using low-temperature picosecond time-resolved fluorescence spectroscopy. The results are compared between moss thalli samples harvested in Antarctica and in Japan. Both samples show almost the same quenching properties, suggesting an identical drought tolerance mechanism for the same species with two completely different habitats. A global target analysis was applied to a large set of data on the fluorescence-quenching dynamics for the 430-nm (chlorophyll-a selective) and 460-nm (chlorophyll-b and carotenoid selective) excitations in the temperature region from 5 to 77 K. This analysis strongly suggested that the quencher is formed in the major peripheral antenna of photosystem II, whose emission spectrum is significantly broadened and red-shifted in its quenched form. Two emission components at around 717 and 725 nm were assigned to photosystem I (PS I). The former component at around 717 nm is mildly quenched and probably bound to the PS I core complex, while the latter at around 725 nm is probably bound to the light-harvesting complex. The dehydration treatment caused a blue shift of the PS I emission peak via reduction of the exciton energy flow to the pigment responsible for the 725 nm band.  相似文献   

18.
Francis-Andr  Wollman  Pierre Bennoun 《BBA》1982,680(3):352-360
A new chlorophyll-protein complex, CP O, was isolated from Chlamydomonas reinhardii using lithium dodecyl sulfate polyacrylamide gel electrophoresis run at 4°C. A similar complex is recovered using Triton/digitonin solubilization of thylakoid membranes of the F54-14 mutant lacking in CP I and ATPase. CP O is enriched in long-wavelength chlorophyll a and contains five polypeptides (27.5, 27, 25, 23 and 19 kDa). Its 77 K fluorescence emission spectrum peaks at 705 nm while CP II have an emission maximum at 682 and 720 nm, respectively. Comparison of the polypeptide pattern of the wild type and AC40 mutant of C. reinhardii shows that the five CP O polypeptides are specifically lacking in the mutant. Although the 77 K emission originating from the Photosystem (PS) I pigments is lower in the mutant than in the wild type, the two spectra show the same peaks at 686, 694 and 717 nm. However, comparison of the 77 K emission spectrum of the F14 mutant lacking in CP I with that of the double mutant AC40-14 lacking in CP I and CP O shows the absence in the latter of the large emission band peaking at 707 nm. The 707 nm emission is thought to arise from some PS I antennae and is quenched in the wild type by the presence of PS I traps located in CP I. We conclude that CP O is a part of the PS I antenna in C. reinhardii which controls the 707 nm fluorescence emission.  相似文献   

19.
1. Skin and rectal temperatures were recorded continuously in 70 measurements during typical tasks of infantry and artillery training at 0 to −29 °C. The duration of the measurements varied from 55 min to 9.5 h.

2. The distribution of finger skin temperatures was quite similar at ambient temperature ranges 0 to −10 °C and −10 to −20 °C, while at −20 to −30 °C the finger temperatures were clearly lower.

3. At different ambient temperature ranges, 20–69% of finger temperatures were low enough to cause cold thermal sensations.

4. Sensation of cold was experienced at a finger temperature of 11.6±3.7 °C (mean±SD).  相似文献   


20.
The role of the hydroxyl group of tyrosine 6 in the binding of Schistosoma japonicum glutathione S-transferase has been investigated by isothermal titration calorimetry (ITC). A site-specific replacement of this residue with phenylalanine produces the Y6F mutant, which shows negative cooperativity for the binding of reduced glutathione (GSH). Calorimetric measurements indicated that the binding of GSH to Y6F dimer is enthalpically driven over the temperature range investigated. A concomitant net uptake of protons upon binding of GSH to Y6F mutant was detected carrying out calorimetric experiments in various buffer systems with different heats of ionization. The entropy change is favorable at temperatures below 26 °C for the first site, being entropically favorable at all temperatures studied for the second site. The enthalpy change of binding is strongly temperature-dependent, arising from a large negative ΔC°p1=−3.45±0.62 kJ K−1 mol−1 for the first site, whereas a small ΔC°p2=−0.33±0.05 kJ K−1 mol−1 for the second site was obtained. This large heat capacity change is indicative of conformational changes during the binding of substrate.  相似文献   

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