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1.
A novel actinomycete, designated strain KLBMP 1262 T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262 T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262 T is most closely related to Amycolatopsis sulphurea DSM 46092 T (97.96 %), Amycolatopsis ultiminotia RP-AC36 T (97.50 %) and Amycolatopsis jejuensis N7-3 T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H 4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C 16:0, iso-C 16:0 and iso-C 15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262 T (=KCTC 19885 T = NBRC 108679 T). 相似文献
2.
A novel Gram-positive, motile, non-spore-forming coccus-shaped bacterial strain, designated KLBMP 1274 T, was isolated from a halophytic plant ( Limonium sinense) collected from the coastal region of Nantong, Jiangsu Province, in east China. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain KLBMP 1274 T belongs to the genus Kineococcus and is closely related to Kineococcus rhizosphaerae RP-B16 T (98.72 %), Kineococcus aurantiacus IFO 15268 T (98.71 %), Kineococcus radiotolerans SRS30216 T (98.69 %) and Kineococcus gynurae KKD096 T (97.33 %). The 16S rRNA gene sequence similarity to other species of the genus Kineococcus was <97 %. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid, with arabinose and galactose as the characteristic sugars. The predominant menaquinone was MK-9(H 2). The polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown phospholipid, an unknown glycolipid, and three unknown lipids. Major cellular fatty acids were found to be anteiso-C 15: 0 and iso-C 14: 0. The chemotaxonomic data for strain KLBMP 1274 T were typical of the genus Kineococcus. The total DNA G+C content was 73.4 mol %. DNA–DNA relatedness and differential phenotypic data demonstrated that strain KLBMP 1274 T was clearly distinguished from all closely related species of the genus Kineococcus. Thus, strain KLBMP 1274 T represents a novel species of the genus Kineococcus, for which the name Kineococcus endophytica sp. nov. is proposed. The type strain is KLBMP 1274 T (=KCTC 19886 T = NBRC 108674 T). 相似文献
3.
A Gram-positive, aerobic, non-motile, non-acid-alcohol-fast strain, designated YIM 61095(T), was isolated from the root of Maytenus austroyunnanensis collected from a tropical rainforest of Xishuangbanna in Yunnan Province, south-west China. Strain YIM 61095(T) exhibited chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61095(T) was a member of the genus Saccharopolyspora and was most closely related to Saccharopolyspora flava AS 4.1520(T) (97.7% sequence similarity). The major fatty acids were iso-C(15:0), iso-C(16:0), iso-C(17:0) and anteiso-C(17:0). The predominant quinone detected was MK-9(H(4)). The DNA G+C content was 66.2 mol%. The phenotypic characteristics and DNA-DNA hybridization relatedness data indicated that strain YIM 61095(T) should be distinguished from Saccharopolyspora flava AS 4.1520(T). On the basis of the evidence presented in this study, strain YIM 61095(T) represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora endophytica sp. nov. is proposed. The type strain is YIM 61095(T) (=KCTC 19397(T)=CCTCC AA 208003(T)). 相似文献
4.
An endophytic actinomycete strain, designated Hhs.015 T, was isolated from roots of cucumber seedlings. The endophytic isolate was identified by means of a polyphasic taxonomic
approach. On the basis of 16S rRNA gene sequence similarities, strain Hhs.015 T was closely related to members of the genus Saccharothrix. DNA–DNA hybridization with the four closest relatives, Saccharothrix longispora NRRL B-16116 T, Saccharothrix xinjiangensis NRRL B-24321 T, Saccharothrix
autraliensis CGMCC 4.1355 T and Saccharothrix espanaensis CGMCC 4.1714 T, gave similarity values of 33.8, 28.2, 44.1 and 29.5%, respectively, which indicated that strain Hhs.015 T represents a novel species of the genus Saccharothrix. This is consistent with the morphological, physiological and chemotaxonomic data. As a whole, these results suggest that
strain Hhs.015 T represents a novel Saccharothrix species. The name Saccharothrix yanglingensis sp. nov. is proposed, with the type strain Hhs.015 T (=CGMCC 4.5627 T = KCTC 19722 T). 相似文献
5.
A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained ll-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C 18:1ω9 c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135 T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA–DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). 相似文献
7.
A novel Gram-strain positive, aerobic, actinobacterial strain, designated CF11/1 T, was isolated from a sand sample obtained in the Sahara Desert, Chad. The black-pigmented isolate was aerobic and exhibited optimal growth from 25 to 35 °C at pH 6.0–8.0 and with 0–8 % (w/v) NaCl, indicating that it is a halotolerant mesophile. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The G+C content in the genome was 74.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol; MK-9(H 4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C 16:0. Analysis of 16S rRNA gene sequences showed 95.3–98.6 % pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus africanus, with the type strain CF11/1 T (DSM 45422 = CCUG 62969 = MTCC 11556). 相似文献
8.
A Gram-positive, aerobic, non-motile actinobacterial strain, designated YIM C01235 T, was isolated from a soil sample collected from the Swallow Cave, Yunnan province, south-west China. The isolate grew at 10–30 °C, pH 6.0–9.0 and 0–8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarity to Saccharopolyspora gloriosae YIM 60513 T (96.8 %), and lower 16S rRNA gene sequence similarities (95.1–96.7 %) with the other species of the genus Saccharopolyspora. The whole-cell hydrolysates contained meso-diaminopimelic acid ( meso-DAP), arabinose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unknown glycolipids, two unknown phospholipids and one polar lipid. MK-9(H 4) was the predominant menaquinone. The major fatty acids were iso-C 15:0, iso-C 16:0, anteiso-C 15:0 and anteiso-C 17:0. Mycolic acids were not detected. The genomic DNA G+C content was 69.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM C01235 T to the genus Saccharopolyspora. The results of physiological and biochemical tests allowed strain YIM C01235 T to be differentiated phenotypically from all the recognized Saccharopolyspora species. On the basis of evidence from this polyphasic study, the novel species Saccharopolyspora cavernae sp. nov. is proposed. The type strain is YIM C01235 T (=DSM 45825 T = CCTCC AA 2012022 T). 相似文献
9.
A novel bacterial strain, TRM F109 T, was isolated from hypersaline habitat in Sichuan Province, China. It was a Gram-positive, aerobic, non-motile, halotolerant, filamentous bacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.4 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain TRM F109 T within the genus Isoptericola. The low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain TRM F109 T represents a novel species of the genus Isoptericola, for which the name Isoptericola salitolerans sp. nov. is proposed. The type strain is TRM F109 T (=JCM 15901 T=KCTC 19617 T). 相似文献
10.
A novel isolate, designated strain KLBMP 1282 T was isolated from the surface-sterilized leaves of a coastal halophyte Tamarix chinensis Lour., collected from Nantong, Jiangsu Province, east of China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this strain belongs to the genus Pseudonocardia, being most closely related to Pseudonocardia kongjuensis LM 157 T (98.33 %), Pseudonocardia autotrophica IMSNU 20050 T (97.77 %), Pseudonocardia endophytica YIM 56035 T (97.63 %), Pseudonocardia ammonioxydans H9 T (97.62 %) and Pseudonocardia compacta IMSNU 20111 T (97.56 %); similarity to other type strains of the genus Pseudonocardia was <97.5 %. Chemotaxonomic data confirmed the affiliation of strain KLBMP 1282 T to the genus Pseudonocardia. Strain KLBMP 1282 T contained MK-8(H 4) as the predominant ubiquinone and iso-C 16:0 as the major fatty acid. The polar lipids detected in strain KLBMP 1282 T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, one unknown phospholipid and four unknown glycolipids. The DNA G + C content of strain KLBMP 1282 T was 73.1 mol %. The results of DNA–DNA hybridizations and the phylogenetic analysis, together with the phenotypic and biochemical tests, allowed the differentiation of strain KLBMP 1282 T from strains of other recognized Pseudonocardia species. Therefore, strain KLBMP 1282 T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia nantongensis sp. nov. is proposed. The type strain is KLBMP 1282 T (=KCTC 29053 T = NBRC 108677 T). 相似文献
11.
A novel Gram-positive, aerobic, rod-shaped and mycelia-producing bacterial strain, designated KLBMP 1050 T, was isolated from the stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed
that the isolate KLBMP 1050 T belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides albus KCTC 9186 T (99.38 %) and Nocardioides luteus KCTC 9575 T (99.03 %). However, the DNA–DNA relatedness of isolate KLBMP 1050 T to these two type strains were 37.5 ± 3.5 and 33 ± 2.3 %, respectively. Strain KLBMP 1050 T grew at the pH range 6–11, temperature range 10–32 °C and with 0–12 % NaCl. The physiological properties of strain KLBMP
1050 T differ from those of N. albus KCTC 9186 T and N. luteus KCTC 9575 T. The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-8(H 4) was the major respiratory quinone. The predominant cellular fatty acid of strain KLBMP 1050 T was iso-C 16:0 (23.3 %). The total DNA G+C content was 70.1 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data,
strain KLBMP 1050 T represents a novel species of the genus Nocardioides, for which the name Nocardioides panzhihuaensis sp. nov. is proposed. The type strain is KLBMP 1050 T (= KCTC 19888 T = NBRC 108680 T). 相似文献
12.
Antonie van Leeuwenhoek - A novel endophytic actinomycete, designated strain BSK3Z-2 T, was isolated from a surface-sterilised branch of Avicennia mariana from Shankou Mangrove Nature... 相似文献
15.
Two novel actinobacterial strains, MS1-9 T and NGC1-4, were isolated from roots of Musa (ABB) cv. ‘Kluai Namwa’, collected from Chachoengsao province, and Musa (ABB) cv. ‘Kluai Chang’, from Suphan Buri province, Thailand, respectively. Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B ( gyrB) gene and whole-genome sequences emphasised that the strains MS1-9 T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363 T, M. krabiensis JCM 12869 T and M. avicenniae DSM 45758 T, respectively. Strains MS1-9 T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan. Whole-cell sugars were glucose, xylose, mannose, and ribose. The acyl type of peptidoglycan was glycolyl. MK-10(H 6), MK-9(H 6), and MK-10(H 8) were presented as the major menaquinones. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles. The major cellular fatty acids consisted of iso-C 15:0, anteiso-C 15:0, anteiso-C 17:0, iso-C 17:0 and C 17:0. The DNA G + C content of strains MS1-9 T and NGC1-4 were 72.2 and 72.3 mol%, respectively. Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9 T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp. nov. is proposed. The type strain is MS1-9 T (=JCM 32149 T = TISTR 2659 T). 相似文献
18.
During the investigation of exploring potential sources of novel species and natural bioactives, a novel actinomycete, designated strain HIT-DPA4T, was isolated from a soil sample, which was collected from Nanjing, Jiangsu Province, PR China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and the result of phylogenetic analysis, strain HIT-DPA4T was most closely related to Streptomyces cyaneus CGMCC 4.1671 T, and shared the highest sequence similarity of 98.76%. In addition, the cell walls of the species HIT-DPA4T contained LL-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were identified as glucose and ribose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and phosphatidylmonomethylethanolamine. MK-9(H6) and MK-9(H4) were predominant menaquinones; and C16:0, anteiso-C15:0 and C15:0 as major cellular fatty acids of the organism HIT-DPA4T. Gene Ontology database analysis and antiSMASH server predicted results displayed that strain HIT-DPA4T was a promising classification units, which has various types of functions and contains multiple biosynthetic gene clusters with the similarity more than 80%. Multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) illustrated that Streptomyces luteolifulvus formed a separate branch in the genus Streptomyces. However, a combination of low level of DNA-DNA relatedness and physiological properties indicated that strain HIT-DPA4T can be distinguished from its phylogenetically related species Streptomyces cyaneus CGMCC 4.1671 T. Moreover, gene synteny research could be further differed organism HIT-DPA4T from similarity species. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces luteolifulvus sp. nov. is proposed. The type strain is HIT-DPA4T (=?CGMCC 4.7558 T?=?TISTR 2751 T).
相似文献
19.
A halotolerant actinomycete strain, designated XHU 5031 T, was isolated from a salt lake in Xinjiang Province, northwest China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense DSM 15700 T (98.4 %), Myceligenerans halotolerans XJEEM 11063 T (98.0 %) and Myceligenerans crystallogenes DSM 17134 T (97.5 %). However, it had relatively low values for DNA–DNA relatedness with the above strains (46.2, 39.4 and 36.5 %, respectively). The peptidoglycan type was A4α. This organism contained glucose, mannose and galactose as the major whole cell sugars. The predominant menaquinone was MK-9(H 4). The major fatty acids were iso-C 15:0, anteiso-C 15:0 and iso-C 16:0. The polar lipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), one unknown phospholipid (PL) and two unknown glycolipids (GL). The G+C content of genomic DNA was 71.2 mol %. Phenotypic data clearly distinguished the isolate from its closest relatives. The combined genotypic and phenotypic data indicated that the isolate XHU 5031 T represented a novel species of the genus Myceligenerans. The proposed name for this organism is Myceligenerans salitolerans sp. nov., with type strain XHU 5031 T (=KCTC 29128 T = CCTCC AB 2012908 T). 相似文献
20.
Two novel anthraquinones, lupinacidins A (1) and B (2), have been isolated from the culture broth of a new endophytic actinomycete belonging to the genus Micromonospora. Lupinacidins were found to show significant inhibitory effects on the invasion of murine colon 26-L5 carcinoma cells without inhibiting cell growth. 相似文献
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