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1.
Richard W. Dapson 《Population Ecology》1972,13(2):161-169
An accurate age-estimating technique, based on biochemical changes in eye lens protein, was used to study age structures of six populations of the old-field mouse, Peromyscus polionotus. A new mathematical procedure permitted quantitative comparisons of these populations. Four inland populations had essentially the same median ages (75–84 days), maximum ages (248–297 days) and relative production rates (56–58% of the surviving population had been born in the 100 days prior to sampling). Approximately 50% of the females were old enough to have weaned a litter. One inland population had a lower median age (49 days), a lower maximum age (181 days) and a higher relative production rate (73%). Relatively fewer females (38%) could have weaned a litter. The single beach population was also different, with higher median age (180 days), and lower relative production rate (2%). All females were old enough to have weaned a litter. Maximum age was essentially the same as for the four similar, inland populations. Factors which may have contributed to observed differences in these populations are discussed. 相似文献
2.
Summary Quantitation of synapses at different postnatal ages has been undertaken in the cerebral cortex of the rat. In this study axial ratios of presynaptic bags, proportion of cortex occupied by presynaptic bags and numbers of synapses per unit volume of cortex have been estimated. Observations on synaptic vesicle packing densities have also been made.Synaptic bags become increasingly spherical up to 7 days of age and become more elongated thereafter. The proportion of cortex occupied by presynaptic bags increases rapidly up to 7 days of age and then at a decelerated rate up to maturity. The number of synapses per unit volume increases slowly over the first four days after which there is a rapid increase to 14 days, followed by a decelerated rate.The average presynaptic bag shows marked changes in volume with increasing age which indicate the probability of two stages of synaptic development. This two stage development is further reflected in the estimates on vesicle packing densities. The implications of the results are discussed in relationship to changes in functional activity of the cortex during postnatal development.The authors wish to express their thanks to Mr. R. Birchenough and Mr. J. Manston for much technical assistance. 相似文献
3.
Estradiol receptors (ER) in mammary glands of female Holtzman rats, either intact or neonatally pinealectomised and housed in 10:14 or 24:0 L:D (light:dark) schedule were studied at 30, 40, 50 +/- 5 and 65 +/- 5 days of age. Whereas ER were detectable only at the age of 60-65 days in intact rats housed in 10:14 L:D, they were present as early as 30 days onwards in the pineal ablated group. In the 24:0 L:D pinealectomised group, though mammary gland ER were maximum around 40 days of age and temporarily undetectable around day 50, they had stabilized around the age of 60-65 days. The data demonstrates for the first time, the modulation of ER in rat mammary glands in response to varying photoperiods as well as pineal ablation. Earlier reports on incidence of chemically induced mammary tumours have been compared to the receptor modulation. 相似文献
4.
Hsp72 is present in plasma from Holstein-Friesian dairy cattle, and the concentration level is repeatable across days and age classes
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Although heat shock proteins (Hsps) are primarily considered as being intracellular, this study identified the presence of Hsp72 in plasma from female Holstein-Friesian dairy cattle. Plasma samples were collected from the same animals at different ages and on different days after calving and accordingly divided into 5 age classes. The age classes were calves less than 235 days of age, young heifers between 235 and 305 days of age, older heifers between 305 and 560 days of age, cows early in lactation, and cows later in lactation. For a subsample of animals within each age class, replicate plasma samples were collected from 1 to 7 days apart to test whether the Hsp72 concentration levels are repeatable on this shorter timescale. Hsp72 was observed in plasma samples from animals of all 5 age classes. For animals with blood samples taken a few days apart, the repeatability (within age class) of the Hsp72 concentration was 0.52 +/- 0.06. Age and days from calving significantly affected the Hsp72 concentration level. The highest Hsp72 level was observed in older heifers (305-560 days of age). The repeatability of Hsp72 concentrations across age classes within animal was 0.22 +/- 0.06. High environmental sensitivity and negative genetic associations between production and health traits in this high-producing breed have been documented earlier. Hsp72 is believed to be strictly stress inducible, and the finding of Hsp72 in plasma indicates that even apparently healthy individuals may experience extrinsic or intrinsic stress (or both). 相似文献
5.
Rat kidney sialidase levels have been reported to be markedly altered in pathological states such as diabetes. This was associated with a modification of sialic acid levels. Therefore, it was interesting to study the variations of kidney sialidase and sialyltransferase activities and sialic acid content according to sex and age. This was carried out from birth to 210 days of age. The substrates used were sialyl alpha(2-3)[3H]-lactitol for sialidase activity, asialofetuin and [14C]-CMPNeu5Ac for sialyltransferase activity. In males sialidase activity increased until 32 days then slightly declined. In females, the activity increased and leveled off at 135 days of age. Higher sialidase activity was observed in females than in males from 56 days of age. Gonadectomy had no effect on this activity. In both sexes, sialyltransferase activity decreased markedly with age. This activity was higher in females than in males, whereas sialic acid levels varied only moderately with age and were slightly higher in females. 相似文献
6.
Three fractions (one soluble and two membrane-bound) of choline acetyltransferase (ChAT) isolated from a nerve ending fraction of mouse forebrain, which have previously been reported to differ in several biochemical and physical aspects, were also found to differ in their rates of postnatal development. At 2 days of age, the activity in all three fractions was very low. Sodium phosphate buffer-soluble (cytoplasmic) ChAT activity increased significantly by 8 days of age, whereas the ChAT activity of the two membrane-bound fractions (NaCl- and Triton-soluble) did not increase until 13 days of age. These results suggested that the differences observed between the three fractions of ChAT prepared from mouse brain are not solely artifacts of the isolation procedure. 相似文献
7.
An unexplained dichotomy exists between the LH (luteinizing hormone) responses to castration of male and female rats, as males show a more prompt increase in serum LH levels. We have tested the hypothesis that neonatal exposure to androgen determines the sexual dimorphism of that response. Control groups of male and female rats were castrated at 60 days of age. Other animals had been castrated at 0 or 25 days of age and then given steroid treatment via testosterone (T) implants from 25 through 60 days of age. At 60 days of age a blood sample was taken from each animal before removal of either the T implant or the gonads. Animals were bled again 24 and 48 h later. Within 24 h after orchidectomy the typical early plateau of plasma LH had occurred, represented by an increment in mean LH concentrations of 316 ng/ml. Orchidectomy at 25 days of age had little or no effect on subsequent response to removal of T. In contrast, neonatal orchidectomy resulted in a markedly diminished response to T removal on Day 60. The response, however, was not reduced to that of normal females. In female rats plasma LH does not increase by 48 h after ovariectomy. Perinatal testosterone propionate (TP) treatment of females partially masculinized (enhanced) the LH response to T implant removal, but only if ovariectomy had been performed prior to puberty (at 0 or 25 days of age).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
8.
Hildebrandt T Drews B Gaeth AP Goeritz F Hermes R Schmitt D Gray C Rich P Streich WJ Short RV Renfree MB 《Proceedings. Biological sciences / The Royal Society》2007,274(1608):323-331
Elephants have the longest pregnancy of all mammals, with an average gestation of around 660 days, so their embryonic and foetal development have always been of special interest. Hitherto, it has only been possible to estimate foetal ages from theoretical calculations based on foetal mass. The recent development of sophisticated ultrasound procedures for elephants has now made it possible to monitor the growth and development of foetuses of known gestational age conceived in captivity from natural matings or artificial insemination. We have studied the early stages of pregnancy in 10 captive Asian and 9 African elephants by transrectal ultrasound. Measurements of foetal crown-rump lengths have provided the first accurate growth curves, which differ significantly from the previous theoretical estimates based on the cube root of foetal mass. We have used these to age 22 African elephant foetuses collected during culling operations. Pregnancy can be first recognized ultrasonographically by day 50, the presumptive yolk sac by about day 75 and the zonary placenta by about day 85. The trunk is first recognizable by days 85-90 and is distinct by day 104, while the first heartbeats are evident from around day 80. By combining ultrasonography and morphology, we have been able to produce the first reliable criteria for estimating gestational age and ontological development of Asian and African elephant foetuses during the first third of gestation. 相似文献
9.
Widely differing ranges for the preferred first-mating age for rats have been reported by different investigators. As well as being of physiological interest this information is important for optimum utilization of animal resources. Previously published results, however, do not provide sufficient information on mortality and the numbers of pups born and weaned. We studied 1623 pups born to 36 female Wistar rats. Dams were first mated at the age of 35 days or 70 days or 105 days. The results show that reproduction and weaning weights increase with an increased first-mating age of dams, although the pup mortality rate also increases. This study can be used when a programme for the production and supply of Wistar rats is planned. 相似文献
10.
Susceptibility and development of Clinostomum complanatum in the chicken (Gallus gallus domesticus) have been studied. The progenetic metacercariae of C. complanatum developed into ovigerous worms in 3 days in the buccal cavity of the chicken and survived only 6-7 days in this experimental host. The susceptibility is influenced by the age, sex and breed/type of the host. Both percent susceptibility and recovery of the worms decreased with increase in the age of the host. The egg laying chickens were found to be least susceptible to the infection. Mature male chickens were more susceptible than the females. The single comb white Leghorn (SCWL) was found to be susceptible while the local type (LT) and the cross breed (SCWLxLT) were not susceptible to C. complanatum. The possible reasons for these variations have been discussed. 相似文献
11.
The effects of ethylene dibromide (EDB) exposure to male rats on several neurotransmitter enzymes have been examined in various brain regions of the F1 progeny, from 7 to 90 days of age. The choline acetyltransferase activity was significantly increased at 21 days old, in most brain regions studied in the F1 progeny of the EDB-treated males, but not at 7, 14 or 90 days old. The acetylcholinesterase activity was altered in different brain regions of the F1 progeny of the EDB-exposed males at both 14 and 21 days old but not at 7 or 90 days old. Glutamic acid decarboxylase activity was increased in corpus striatum but decreased in frontal cortex only at 21 days of age. These neurochemical changes in the developing brain of F1 progeny of EDB-treated males at low doses may be associated with behavioral abnormalities observed early in their development. 相似文献
12.
Floral determination in the terminal bud of the short-day plant Nicotiana tabacum cv. Maryland Mammoth has been investigated. Plants grown continuously in short days flowered after producing 31.4±1.6 (SD) nodes while plants grown continuously in long days did not flower and produced 172.5±9.5 nodes after one year. At various ages, expressed as number of leaves that were at least 1.0 cm in length above the most basal 10-cm leaf, one of three treatments was performed on plants grown from seed in short days: 1) whole plants were shifted from short days to long days, 2) the terminal bud was removed and then rooted and grown in long days, and 3) the terminal bud was removed and then rooted and grown in short days. Whole plants flowered only when shifted from short days to long days at age 15 or later. Only rooted terminal buds from plants at age 15 or older produced plants that flowered when grown in long days. Only terminal buds from plants at age 15 or older that were rooted and grown in short days produced the same number of nodes as they would have produced in their original locations while buds from younger plants produced more nodes than they would have in their original locations. Thus, determination for floral development in the terminal bud, as assayed by rooting, is simultaneous with the commitment to flowering as assayed by shifting whole plants to non-inductive conditions.Abbreviations LD
long day(s)
- SD
short day(s)
- DN
dayneutral 相似文献
13.
Temperature and mass dependency of insect metabolic rates are well known, while less attention has been given to other factors, such as age. Among insect species that experience seasonal variation in environmental conditions, such as in temperate latitudes, age may also have indirect effects on the metabolic rate. We examined the effect of age on the resting metabolic rate of Leptinotarsa decemlineata during 11 days after adult emergence by using flow-through respirometry. Age had a significant mass-independent effect on metabolic rate of beetles. A twofold increase in metabolic rate occurred during the first 2 days of adult life after which metabolic rate decreased with age relatively slowly. Ten day-old adult beetles had a metabolic rate similar to newly emerged beetles. The beetles have to be able to complete their development and prepare for overwintering during the relatively short favourable summer periods. Therefore, the observed pattern in metabolic rate may reflect physiological changes in the pre-diapause beetles adapted to temperate latitudes. 相似文献
14.
M Petkó 《Acta morphologica Academiae Scientiarum Hungaricae》1975,23(2):123-131
Morphological and histochemical changes of ultimobranchial follicles of thyroid have been investigated in rats from newborn to 18 months of age. The first well-delimited ultimobranchial follicles, though with no lumen, were detected in the thyroid gland of 10-day-old rats. At 30 days of age, follicles possessing regular lumina were present in the thyroid. With age, the follicles gradually increased in volume assuming extreme dimensions in adult age. The follicles displayed varying shapes from simple cysts to bizarre forms. From the age of 50 days the cells of the follicular wall are separated from the cell debris contained in the lumen. The latter gave a PAS positive reaction. The cells of the ultimobranchial follicles did not exhibit argyrophilia and metachromasia showing that they differ considerably from the C-cells likewise of ultimobranchial origin, which are known to give marked argyrophilic and metachromatic reactions. 相似文献
15.
M R Simon 《Acta anatomica》1983,117(4):339-345
Daily subcutaneous injections of glucocorticoid preparations have been shown to produce clinical signs of hyperadrenocorticism which resulted in interference with the growth of rats, shown by measurements both of body weight and of the length of long bones. The purpose of this study was to see if increased, intermittent, compressive forces, produced by experimental bipedalism, would mitigate the negative effects of cortisone injections on long bone growth and if there would be a difference in reaction between male and female rats. Male and female Sprague-Dawley rats were used for control, cortisone-injected, and cortisone-injected plus bipedal groups. Experimental bipedalism was produced at 10 days of age; cortisone injections began at 30 days of age; all animals were sacrificed at 65 days of age. Tibial and femoral lengths were measured. The results do not support the hypothesis that bipedalism can be instrumental in mitigating the effects of hyperadrenocorticism on hindlimb long bone growth. 相似文献
16.
R J Cross J S Bryson T L Roszman 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(5):1347-1352
The Snell-Bagg hypopituitary dwarf mouse has been shown to be deficient in growth hormone, thyroxine, and prolactin. There are reports indicating that in addition to these neuroendocrine abnormalities, development of immune competence is also severely impaired in these animals. However, other studies indicate that the immunologic potential of these mice does not differ from their heterozygous littermate controls. Our data show that dwarf mice weaned at 21 days of age and killed at that time, or 7 days later, have reduced numbers of cells in both the spleen and thymus and the mitogen responsiveness of these cells is impaired. However, if mice weaned on day 21 are analyzed at 32 days of age or the mice are weaned at day 30 and analyzed 7 days later the ability to respond to mitogenic stimulation does not differ from controls. Further experiments show that dwarf mice weaned at 30 days of age have a normal complement of V-beta TCR as evidenced by immunofluorescence analysis as well as a primary antibody response to SRBC equivalent to that observed in normal littermates. Immunofluorescence analysis of CD4 and CD8 expression on thymocytes obtained from dwarf mice shows a distinct pattern dependent on the time of weaning and time of analysis. Initial analysis of thymocytes from dwarf mice weaned and killed at 21 days of age do not differ from controls. However, cells from dwarf mice weaned on day 21 and killed on day 28 are markedly different with a loss of immature CD4+/CD8+ cells and a corresponding increase in CD4+ and CD8+ mature thymocytes. In contrast, the phenotype of thymocytes obtained from dwarf mice weaned at 30 days of age and killed on day 37 did not differ from normal littermates. Collectively these studies indicate that hypopituitary dwarf mice lag behind their heterozygous littermates with respect to development of immunocompetence but normal immune responsiveness does develop by 32 days of age when the mice are weaned on day 21. 相似文献
17.
K Okumura Y Yamada J Kondo A Ishida H Hashimoto T Ito K Ogawa J Kitoh 《Life sciences》1988,43(17):1371-1377
1,2-Diacylglycerol (DAG) has been suggested to be a secondary messenger. In this study, we determined the amount of 1,2-DAG in heart tissue from Syrian hamsters with hereditary cardiomyopathy at 30 days (prenecrotic stage) and 90 days of age by thin-layer chromatography with flame ionization detection (TLC-FID). Myocardial triglyceride contents were higher at 30 days of age and lower at 90 days of age compared to the levels in age-matched normal hamsters. Decreases in major species of phospholipids in hearts were observed only at 90 days of age. However, elevated 1,2-DAG content in myopathic hearts was found at 30 days of age, whereas there was no difference between the two groups at 90 days of age. It is suggested that the increase in 1,2-DAG at the prenecrotic stage is involved in the pathogenesis of the cardiomyopathy. 相似文献
18.
In a number of animal species it has been shown that exposure to low levels of stress at a young age has a positive effect
on stress resistance later in life, and on longevity. The positive effects have been attributed to the activation of defence/cleaning
systems (heat shock proteins (Hsps), antioxidases, DNA repair) or to effects of a changed metabolic rate, or both. We investigated
the effect of mild stress exposures early in life on Hsp70 synthesis after a harder stress exposure later in life in five
isofemale lines ofDrosophila melanogaster. Female flies were either exposed to repeated bouts of mild heat stress (3 h at 34‡C) at a young age (days 2, 4 and 6 post-eclosion)
or held under standard laboratory conditions. At 16 and 32 days of adult age, respectively, flies were exposed to a high temperature
treatment known to induce Hsp70 in the investigated species (1 h at 37‡C). Thereafter, the inducible Hsp70 levels were measured.
Our data show a tendency towards increased Hsp70 synthesis with increased age for both ’mild stress’ and ’no stress’ flies.
Moreover, the results show that flies exposed to mild stress at a young age synthesized more Hsp70 upon induction, compared
to control flies, and that this difference was accentuated at 32 days compared to 16 days of age. Thus, bouts of mild heat
stress at a young age impact on the physiological stress response system later in life. This may be caused by an increased
ability to react to future stresses. Alternatively, the mild stress exposure at a young age may actually have caused cellular
damages increasing the need for Hsp70 levels after stress exposure later in life. The importance of an Hsp70 upregulation
(throughout life) in explaining the phenomenon of hormesis is discussed, together with alternative hypotheses, and suggestions
for further studies. 相似文献
19.
M D Rubio R Santisteban P Tovar J Ibá?ez F Castejón 《Revista Espanola de Fisiología》1989,45(2):151-155
The effects of cardiac maturation on the orientation of the auricular activation mean vectors (AP and SAP) in Landrace X White Belgian pigs has been analyzed. The magnitudes of AP and SAP vectors undergo a significant increase between 5 and 20 days of age. On the contrary, physical maturation does not appear to have any effect on the sequence of auricular activation in pigs, since in all the age groups analyzed, the same orientation for P vectors on the horizontal plane and in space was maintained. Both vectors indicated that the auricular activation front was predominantly directed towards the left, caudally and ventrally. It should be noted that in a high percentage of individuals of 1 to 5 days of age, the auricular activation vector goes towards the left and cranially. 相似文献
20.
The endocrinologic basis for morphological and biochemical sex differences in the mouse submandibular gland have not been clarified. Previous studies have emphasized the maintenance of glandular differences in adult animals, rather than considering the factors responsible for their developmental etiology. Male CD-1 mice were castrated at intervals between 10 and 50 days of age and killed at 100 days. The quantitative development of granular tubules and the carbohydrate histochemistry of the submandibular glands were compared to untreated males and females. The area of granular tubules increased with age at castration. Nested analysis of variance indicated significant differences among treatments and among sections within individual glands. No group of castrated males had a greater development of tubules than untreated females. Carbohydrate histochemistry demonstrated an increase in carboxylated mucosubstances in the acinar cells and granular tubule cells of castrated animals. 相似文献