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1.
The uptake of glucose and other carbohydrates into the guard cells of Commelina communis L. was found to inhibit the opening of the stomata. The concentration of glucose necessary to achieve about 50% inhibition was of the same order of magnitude as the potassium concentration required for opening; the uptake systems for potassium and glucose appear to be competitive and to exhibit the same degree of affinity. It is suggested that the uptake of glucose occurs via a proton cotransport, which, depolarizing the membrane potential, slows down the electrogenic import of potassium ions. The process of stomatal closure, in contrast, appears not to be affected by carbohydrate uptake. In guard cells of Tulipa gesneriana L. and Vicia faba L., which do not possess subsidiary cells, import of glucose or other carbohydrates did not interfere with the regulation of stomatal movements. 相似文献
2.
H. Schnabl 《Planta》1978,144(1):95-100
Chloride ions are necessary to compensate for the positively charged potassium ions imported into guard cells of Allium cepa L. during stomatal opening. Therefore an external Cl- supply of intact Allium plants is important. But high levels of chloride have been found to reduce the sensitivity of the starch-lacking stomata and isolated guard cell protoplasts (GCPs) from Allium to potassium ions, fusicoccin and abscisic acid. Furthermore, with high levels of chloride, malate anions disappear from the guard cells of Allium, a finding which contrasts with situation in Vicia where the stomatal sensitivity to K+ ions, fusicoccin and ABA is not influenced by Cl- ions and malate levels are unaffected. It is suggested that the absence of malate as a proton yielding primer inhibits the mechanism of H+/K+ exchange in Allium.Abbreviations ABA
abscisic acid
- FC
fusicoccin
- GCPs
guard cell protoplasts 相似文献
3.
Does photorespiration protect the photosynthetic apparatus in french bean leaves from photoinhibition during drought stress? 总被引:15,自引:0,他引:15
Dithiothreitol (DTT), an inhibitor of violaxanthin de-epoxidation and zeaxanthin formation in chloroplasts, inhibited blue-light-stimulated stomatal opening in epidermal peels of Vicia faba L. in a concentration-dependent fashion. Complete inhibition was observed at 3 mM DTT. The DTT effect was specific for the stomatal response to blue light, and the red-light-stimulated opening, which depends on photosynthetic reactions in the guard cells, was unaffected. Preirradiation of stomata in epidermal peels with increasing photon fluence rates of red light, prior to an incubation in 10 mol·m-2·s-1 of blue light and 100 mol·m-2·s-1 red light, resulted in a DTT-sensitive, blue-light-stimulated opening that was proportional to the fluence rate of the red light pre-treatment. Guard cells in epidermal peels and guard-cell protoplasts irradiated with red light showed increases in their zeaxanthin content that depended on the fluence rate of red light, or on the incubation time. The increases in zeaxanthin concentration were inhibited by DTT. The obtained results indicate that zeaxanthin could function as a photoreceptor mediating the stomatal responses to blue light.Abbreviation DTT
dithiothreitol
This work was supported by grants from the National Science Foundation and the US Department of Energy to E.Z. 相似文献
4.
Stomatal movement and potassium transport in epidermal strips of Zea mays: The effect of CO2 总被引:1,自引:1,他引:0
C. K. Pallaghy 《Planta》1971,101(4):287-295
Summary The correlation between stomatal action and potassium movement in the epidermis of Zea mays was examined in isolated epidermal strips floated on distilled water. Stomatal opening in the isolated epidermis is reversible in response to alternate periods of light or darkness, and is always correlated with a shift in the potassium content of the guard cells. K accumulates in guard cells during stomatal opening, and moves from the guard cells into the subsidiary cells during rapid stomatal closure. When epidermal strips are illuminated in normal air, as against CO2-free air, the stomata do not open and there is a virtually complete depletion of K from the stomatal apparatus. In darkness CO2-containing air inhibits stomatal opening and K accumulation in guard cells, but does not lead to a depletion of K from the stomata as observed in the light. 相似文献
5.
Colin M. Willmer 《Protoplasma》1976,87(1-3):253-262
Summary The lower leaf epidermis from 5 plant species was stained with neutral red at 2 pH's (7.1 and 5.6) in the light and dark when the stomata were open or closed. At pH 5.6 no globule (= droplet) formation was observed in the guard cells whether the stomata were open or closed and cell walls possessed a high affinity for the stain. At pH 7.1 globules appeared in guard cells of open stomata, but not closed stomata, within 15 minutes. Anaerobic conditions prevented this globule formation. InZea mays, globules also appeared in subsidiary cells when the stomata were closed and in certain epidermal cells. Where globule formation did not occur increased diffuse staining of certain epidermal cells was considered to be the indication of cell integrity. In old leaf material very large numbers of dark blue globules appeared in epidermal cells ofCommelina diffusa, C. communis andSenecio odoris and this was associated with cell senescence.The staining characteristics were discussed in terms of cellular K+, Cl–, tannin and flavonoid content and vacuolar pH. 相似文献
6.
Both adaxial and abaxial leaf surfaces were searched for stomata inBalanophora elongata, B. fungosa, Hachettea austro-caledonica, Langsdorffia hypogaea, Lophophytum mirabile subsp.mirabile, Scybalium jamaicense, andThonningia sanguinea (Balanophoraceae). Neither stomata nor guard cells were observed. The epidermal surfaces of these species are extremely diverse with respect to cell shape, cell size, and surface ornamentation, these features providing valuable systematic criteria. 相似文献
7.
Guard Cells Extrude Protons Prior to Stomatal Opening--A Study using Fluorescence Microscopy and pH Micro-electrodes 总被引:3,自引:2,他引:1
A method for the demonstration of pH changes in the apoplastis described. The fluorescent pH indicator pnmulin was usedto follow pH changes in the epidermis of leaves of Commelinacommunis during stomatal movements. Previously darkened leavesexposed to light showed quenching of fluorescence in the apoplastsurrounding theuard cells up to 20 min before the stomata opened.This indicated that proton efflux by the guard cells precededstomatal opening. This result was substantiated by apoplasticpH measurements using pH micro-electrodes. Acidification ofthe apoplast spread outwards from the guard cells to the surroundingsubsidiary and epidermal cells. This phenomenon persisted forsome time after subsequent stomatal closure, supporting thehypothesis that closure is brought about by a process otherthan the cessation of proton pumping. Key words: Commelina communis, stomata, proton pumping 相似文献
8.
Summary A chloride selective micro-electrode has been used to determine vacuolar chloride concentrations in individual cells of the leaf epidermis of Commelina communis. When the stomata were open a gradient in chloride concentration across the stomatal complex was observed with the highest concentration in the guard cells. On stomatal closure the chloride gradient was reversed. Calculation of the driving forces on chloride indicated that active transport of chloride was occurring during both stomatal opening and closure. This transport appeared to be energetically independent of the transport of potassium. These results are discussed in relation to the behaviour of other anions during stomatal movements. 相似文献
9.
10.
pH Gradients in the Stomatal Complex of Tradescantia virginiana 总被引:1,自引:0,他引:1
pH of the vacuolar sap, cytoplasm, and apoplast of the cellsof the stomatal complex of Tradescantia virginiana was measuredusing micro-electrodes. Marked differences in vacuolar and apoplasticpH were observed between leaves with open and closed stomata.Cytoplasmic pH appeared to be uniform in all the cells and didnot change with stomatal aperture. The information obtainedenabled proton motive force across the plasmalemma and tonoplastof the guard cells to be calculated. The results are discussedin relation to the accumulation of potassium by the guard cellon stomatal opening. Key words: Stomata, pH gradients, Proton pumps 相似文献
11.
Treatment with cyanide of epidermal peels isolated from pea leaves resulted in destruction of nuclei in the guard cells of stomata, which is visible with a light microscope. The process was accelerated by illumination. Electron microscopy revealed significant CN--induced changes in the ultrastructure of guard cells, which increased with time. Margination of chromatin, which is one of the first signs of apoptosis, was observed in the guard cells even after 1 h incubation of the isolated epidermis with CN-. Subsequent chromatin condensation, swelling of the endoplasmic reticulum with formation of large tanks covered with ribosomes, changes in the structure of dictyosomes, and a slight swelling of mitochondria were observed after 3 h of the epidermis incubation with CN-. After 6 h of incubation with CN-, the bulk volume of the guard cells was filled with vacuoles, the cytoplasm occupied the thin marginal layer, the nucleus was in the center similarly to the control experiment, but it was polylobal, extended in narrow cytoplasmic bands, and, despite the loss of the nuclear envelope integrity, appeared to be a self-dependent structure. In the envelope-free open regions of the nucleus, mitochondria and chloroplasts directly contacted with chromatin. Much like the cell nucleus, chloroplasts lost the integrity of the membrane, but did not swell and retained the stroma and integrity of the thylakoid system. An antioxidant di-tert-butyl-4-hydroxytoluene prevented ultrastructural changes in the cells observed after 6 h of incubation with CN-. Thus, the CN--induced death of the guard cells of stomata occurs through the mechanism of apoptosis. 相似文献
12.
Studies on isolated starch-containing (Vicia faba) and starch-deficient (Allium cepa) guard cell protoplasts 总被引:1,自引:1,他引:0
Guard cell protoplasts from starch-containing Vicia faba and starch-deficient Allium cepa stomata were isolated, stabilized and recovered with an efficiency — in relation to the potential yield — of approx. 62% and 77%, respectively. In vitro, guard cell protoplasts (GCP) respond to abscisic acid and fusicoccin by respectively contracting and swelling, that is, decreasing or increasing in diameter by about 15% and more in comparison to the control. This in vitro response correlates with, but is more than 4 times as rapid as, the in vivo response of the stomata. Among the advantages presented by working with isolated GCPs are: greater sensitivity in response; freedom from influences of cuticular ridges, cell walls, subsidiary cells, and epidermal cells; and direct and parallel comparisons of starch-containing and starch-deficient GCP systems.Abbrecviations ABA
abscisic acid
- FC
fusicoccin
- ECP, MCP, and GCP
epidermal, mesophyll, and guard cell protoplasts, respectively
- PPV
packed protoplast volume 相似文献
13.
The water relations of leaves of Tradescantia virginiana were studied using the miniaturized pressure probe (Hüsken, E. Steudle, Zimmermann, 1978 Plant Physiol. 61, 158–163). Under well-watered conditions cell turgor pressures, P
o, ranged from 2 to 8 bar in epidermal cells. In subsidiary cells P
o was about 1.5 to 4.5 bar and in mesophyll cells about 2 to 3.5 bar. From the turgor pressure, relaxation induced in individual cells by changing the turgor pressure directly by means of the pressure probe, the half-time of water exchange was measured to be between 3 and 100 s for the epidermal, subsidiary, and mesophyll cells. The volumetric elastic modulus, , of individual cells was determined by changing the cell volume by a defined amount and simultaneously measuring the corresponding change in cell turgor pressure. The values for the elastic modulus for epidermal, subsidiary, and mesophyll cells are in the range of 40 to 240 bar, 30 to 200 bar, and 6 to 14 bar, respectively. Using these values, the hydraulic conductivity, L
p, for the epidermal, subsidiary, and mesophyll cells is calculated from the turgor pressure relaxation process (on the basis of the thermodynamics of irreversible processes) to be between 1 and 55·10-7 cm s-1 bar-1. The data for the volumetric elastic modulus of epidermal and subsidiary cells indicate that the corresponding elastic modulus for the guard cells should be considerably lower due to the large volume changes of these cells during opening or closing. Recalculation of experimental data obtained by K. Raschke (1979, Encycl. Plant Physiol. N.S., vol. 7, pp 383–441) on epidermal strips of Vicia faba indicates that the elastic modulus of guard cells of V. faba is in the order of 40–80 bar for closed stomata. However, with increasing stomatal opening, i.e., increasing guard cell volume, decreases. Therefore, in our opinion Raschke's results would indicate a relationship between guard cell volume and which would be inverse to that for plant cells known in the literature. assumes values between 20–40 bar when the guard cell colume is soubled. 相似文献
14.
Effect of Potassium Levels on the Stomatal Behavior of the Hemi-Parasite Striga hermonthica
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The hemi-parasite Striga hermonthica, exhibits an anomalous pattern of stomatal response, stomata remaining open in darkness and when subjected to water stress. This suggests irregularity in stomatal response due to malfunction of the stomatal mechanism. To test this suggestion guard cells were isolated from the effects of surrounding cells, by incubating epidermal strips at low pH. These stomata responded rapidly to low CO2 concentrations, darkness, and ABA. Thus, a paradox exists between stomatal behavior observed in whole leaves and that in isolated guard cells. However, when incubated in the presence of high potassium concentrations (>200 millimolar KCl) stomatal responses in epidermal strips resembled those found in whole leaves, with enhanced opening and reduced closing responses. It is suggested that the anomalous behavior of stomata in Striga and other leafy hemiparasites can be explained by the modulatory effects of high potassium concentrations which accumulate in the leaves as a consequence of high transpiration rates and the lack of a retranslocation system. 相似文献
15.
Samuilov VD Lagunova EM Dzyubinskaya EV Izyumov DS Kiselevsky DB Makarova YV 《Biochemistry. Biokhimii?a》2002,67(6):627-634
The effect of cyanide, an apoptosis inducer, on pea leaf epidermal peels was investigated. Illumination stimulated the CN–-induced destruction of guard cells (containing chloroplasts and mitochondria) but not of epidermal cells (containing mitochondria only). The process was prevented by antioxidants (-tocopherol, 2,5-di-tret-butyl-4-hydroxytoluene, and mannitol), by anaerobiosis, by the protein kinase C inhibitor staurosporine, and by cysteine and serine protease inhibitors. Electron acceptors (menadione, p-benzoquinone, diaminodurene, TMPD, DCPIP, and methyl viologen) suppressed CN–-induced apoptosis of guard cells, but not epidermal cells. Methyl viologen had no influence on the removal of CN–-induced nucleus destruction in guard cells under anaerobic conditions. The light activation of CN–-induced apoptosis of guard cells was suppressed by DCMU (an inhibitor of the electron transfer in Photosystem II) and by DNP-INT (an antagonist of plastoquinol at the Qo site of the chloroplast cytochrome b
6
f complex). It is concluded that apoptosis initiation in guard cells depends on the simultaneous availability of two factors, ROS and reduced quinones of the electron transfer chain. The conditions for manifestation of programmed cell death in guard and epidermal cells of the pea leaf were significantly different. 相似文献
16.
PUROHIT SADHNA; LALORAYA MANMOHAN M.; BHARTI SUDHAKAR; NOZZOLILLO CONSTANCE 《Journal of experimental botany》1992,43(1):103-110
The study of the structure-activity relationship of phenoliccompounds in reversing the ABA-effect on stomata led us to investigatethe changes in K+ concentrations in guard cells and in the epidermaldiffusive resistance of leaves, after treatment with ABA andphenolics. The amount of potassium localized in guard cells usually correspondsto stomatal aperture in different treatments. Umbelliferone,however, permits stomatal opening without retention of potassiumin the guard cells, which is an exception. The effect of phenolicsin retaining K+ in epidermal peels is matched by recorded epidermaldiffusive resistance changes in the leaves.Although flavonoidsand some other phenolics behave differently showing recoveryin epidermal peels with K+ in guard cells, epidermal diffusiveresistance is not recovered. Key words: Epidermal diffusive resistance, K+, ABA, phenolics, stomata 相似文献
17.
Blue light effects on stomata are mediated by the guard cell plasma membrane redox system distinct from the proton translocating ATPase 总被引:2,自引:2,他引:0
A. S. RAGHAVENDRA 《Plant, cell & environment》1990,13(2):105-110
Abstract. The effects of blue light on stomata are critically analysed. Blue-light-induced increase in stomatal conductance is preceded by membrane hyperpolarization, proton efflux, potassium uptake and malate synthesis in guard cells. Hypothetically, a flavin containing plasma membrane redox system can pump protons out of guard cells on illumination with blue light. It is proposed that this electrogenic proton pump requires NAD(P)H but does not involve ATP/ATPase. 相似文献
18.
Isotachophoretic analysis of ions was performed on guard cells of Vicia faba cv. Ryosai Issun with either open or closed stomata. In guard cells of open stomata, K+ and malic acid concentrations were 5–7 and 5–10 times higher, respectively, than in guard cells of closed stomata. The content of citric acid (plus isocitric acid) also increased during stomatal opening, but the increment was smaller than that of malic acid. Sodium ions, phosphoric and glyceric acids were present in low concentrations but did not increase during the opening. Other cations and anions could not be measured because of low concentrations. Malic acid provided 68–79% of the counter anions for the potassium taken up by guard cells during stomatal opening. 相似文献
19.
Summary To obtain a full picture of microtubule (MT) behavior during the opening and closure of guard cells we have microinjected
living guard cells ofVicia faba with fluorescent tubulin, examined fine detail by freeze shattering fixed cells, and used drug treatments to confirm aspects
of MT dynamics. Cortical MTs in fully opened guard cells are transversely oriented from the ventral wall to the dorsal wall.
When the stomatal aperture was decreased by darkness, these MTs became twisted and patched and broken down into diffuse fragments
when stomata were closed. When the closed stomata were opened in response to light, the MTs in guard cells changed from the
diffused, transitional pattern back to one in which MTs are transversely oriented from stomatal pore to dorsal wall. This
observation indicates a linkage between these MT changes and stomatal movement. To confirm this, we used the MT-stabilizing
agent taxol and the MT-depolymerizing herbicide oryzalin and observed their effects on the stomatal aperture and MT dynamics.
Both drugs suppressed light-induced stomatal opening and dark-induced closure. MTs are known to be necessary for maintaining
the static kidney shape of guard cells; the present data now show that the dynamic properties of polymeric tubulin accompany
changes in shape with stomatal movement and may be functionally involved in stomatal movement. 相似文献
20.
In the guard cells of Allium cepa leaves, no starch was found either when the stomata were open or closed. The lack of other soluble polysaccharides that could be hydrolyzed during the opening reaction of the stomata (Schnabl, Planta 1977, in press) leads to the question, how is the osmotic effect, which is the basis of the stomatal movement, achieved in Allium? It is shown in this paper, by histochemical and microprobe analyses, that in Allium — as in other plant species—the K+ concentration of the guard cells increases during stomatal opening. The charges of the K+ ions in the guard cells seem to be fully compensated by imported Cl- ions. This could mean that if starch is present in the guard cells, as in the majority of plant species, its major role in the mechanism of stomatal movement is to deliver the cuunteranions for the imported K+ ions. 相似文献