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1.
Fertile F1 hybrids were obtained between durum wheat (Triticum durum Desf.) Langdon (LDN) and its 10 disomic substitution (LDN DS) lines with Aegilops tauschii accession AS60 without embryo rescue. Selfed seedset rates for hybrids of LDN with AS60 were 36.87% and 49,45% in 2005 and 2006, respectively. Similar or higher selfed seedset rates were observed in the hybrids of ID (1A), 1D (1B), 3D (3A), 4D (4B), 7D (TA), and 2D (2B) with AS60, while lower in hybrids of 3D (3B) + 3BL, 5D (5A) + 5AL, 5D (5B) + 5B and 6D (6B) + 6BS with AS60 compared with the hybrids of LDN with AS60. Observation of male gametogenesis showed that meiotic restitution, both first-division restitution (FDR) and single-division meiosis (SDM) resulted in the formation of functional unreduced gametes, which in turn produced seeds. Both euhaploid and aneuhaploid gametes were produced in Fi hybrids. This suggested a strategy to simultaneously transfer and locate major genes from the ancestral species T. turgidum or Ae. tauschii. Moreover, there was no significant difference in the aneuhaploid rates between the F1 hybrids of LDN and LDN DS lines with AS60, suggesting that meiotic pairing between the two D chromosomes in the hybrids of LDN DS lines with AS60 did not promote the formation of aneuhaploid gametes. 相似文献
2.
The hybrids of durum and bread wheat with Ae. cylindrica have been obtained without using an embryo rescue technique. The hybrid output (of pollinated flower number) in the field conditions scored 1.0, 15.3 and 10.0% in the crosses T. durum x Ae. cylindrica, Ae. cylindrica x T. durum and T. aestivum x Ae. cylindrica, respectively. A high level of meiotic chromosome pairing between homologous D genomes of bread wheat and Aegilops has been revealed (c = 80.0-83.7%). The possibility of homoeological pairing between wheat and Ae. cylindrica chromosomes has been shown. Herewith, the correlation between the levels of homological and homoeological pairing is absent. The possibilities of genetic material interchange, including between the tetraploid species, as well as the using of Ae. cylindrica cytoplasm for durum wheat breeding are discussed. 相似文献
3.
A nuclear gene, Ncc-tmp1A, of Triticum timopheevii is required for the nucleus-cytoplasm (NC) compatibility in tetraploid NC hybrids with the cytoplasm of Aegilops squarrosa. A euploid NC hybrid of T. durum was previously produced by introgressing the gene from chromosome 1A of T. timopheevii. To examine the possible presence of a functional homoeoallele in the G genome of T. timopheevii, segregation of seed viability was studied as a marker phenotype in BC1s involving the two types of NC hybrids, (Ae. squarrosa)-T. timopheevii and (Ae. squarrosa)-T. turgidum. The result of these test crosses suggested that the G genome possesses a functional homoeoallele Ncc-tmp1G. Segregation of two RAPD (random amplified polymorphic DNA) markers that were closely linked to Ncc-tmp1A was further studied among the viable BC1s obtained from a test cross of (Ae. squarrosa)-T. timopheevii x T. turgidum. Some viable BC1 segregants without the markers were obtained, suggesting a limited degree of transmission of chromosome 1G carrying Ncc-tmp1G. However, a similar RAPD analysis of BC1s obtained after backcrosses of reciprocal F1s of T. timopheevii/T. turgidum with T. turgidum showed random marker segregation. Thus, it was concluded that Ncc-tmp1A is not required for compatibility with its own cytoplasm. Southern blot analysis of the euploid NC hybrid using RFLP (restriction fragment length polymorphism) markers on the homologous group 1 chromosomes showed that Ncc-tmp1A locates in the centromeric region. 相似文献
4.
硬粒小麦-粗山羊草双二倍体白粉病抗性的遗传分析与基因推导 总被引:1,自引:0,他引:1
对99份硬粒小麦-粗山羊双二倍体用北京地区流行的5号白粉菌生理小种进行了白粉病抗性鉴定,筛选出11个苗期抗病的双二倍体材料和2个全生育期抗病的材料M53和M81。对M53和M81及其硬粒小麦和粗山羊草亲本进行的抗白粉病鉴定结果表明,其抗性来源于粗山羊草。与M53和M81具有相同硬粒小麦亲本、不同粗山羊草亲本双二倍体的抗性结果也表明抗性基因来源于粗山羊草。对M53和M81的抗性遗传分析表明,它们均携带1个单显性抗病基因。用14个白粉菌生理小种对已知抗病基因品系与M53和M81两份待测材料进行接种鉴定,结果表明,M53和M81与已知基因的抗菌谱均不相同,M53与M81的抗菌谱也不相同,说明M53和M81各自分别携带1个新的显性抗白粉病基因。 相似文献
5.
O. G. Silkova I. G. Adonina E. A. Krivosheina A. I. Shchapova V. K. Shumny 《Sexual plant reproduction》2013,26(1):33-41
The normal course of meiosis depends on regular pairing of homologous chromosomes. In intergeneric hybrids, including those of wheat, there is no chromosome pairing because there are no homologs. In F1 wheat/rye hybrids, pairing is largely prevented by the pairing homoeologous1 (Ph1) gene. In its presence, there are only rare instances of pairing; most chromosomes are univalent, and their orientation at metaphase I initiates different pathways of the meiotic cycle. The meiotic-like pathway includes a combination of the reductional and the equational + reductional steps at AI followed by the second division. The resulting gametes are mostly non-functional. The mitotic-like pathway involves equational division of univalents at AI and the absence of the second division. Any fertility of wheat/rye hybrids depends on the production of unreduced gametes arising from meiotic restitution (mitotic-like division). We examined the meiotic pairing in wheat/rye hybrids created from wheat lines with single rye chromosome substitutions and Ph1 present. This guaranteed F1 meiosis with one pair of rye homologs. All hybrids formed bivalents, but proportions of meiocytes with bivalents varied. In the meiocytes where bivalents were present, there was a higher tendency for the meiotic-like pathway, while in meiocytes where bivalent pairing failed, the tendency was stronger for the mitotic-like pathway. Among the equationally dividing cells, we observed more than 90 % of meiocytes without bivalents, where rye homologs did not form bivalents, too. The data indicate a potential application of wheat/rye lines in producing genetic stocks of amphidiploids with designated genomic constitutions. 相似文献
6.
Matsuoka Y Nasuda S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(8):1710-1717
Hexaploid bread wheat was derived from a hybrid cross between a cultivated form of tetraploid Triticum wheat (female progenitor) and a wild diploid species, Aegilops tauschii Coss. (male progenitor). This cross produced a fertile triploid F1 hybrid that set hexaploid seeds. The identity of the female progenitor is unknown, but various cultivated tetraploid Triticum wheats exist today. Genetic and archaeological evidence suggests that durum wheat (T. turgidum ssp. durum) may be the female progenitor. In previous studies, however, F1 hybrids of durum wheat crossed with Ae. tauschii consistently had low levels of fertility. To establish an empirical basis for the theory of durum wheat being the female progenitor of bread wheat, we crossed a durum wheat cultivar that carries a gene for meiotic restitution with a line of Ae. tauschii. F1 hybrids were produced without using embryo rescue techniques. These triploid F1 hybrids were highly fertile and spontaneously set hexaploid F2 seeds at the average selfed seedset rate of 51.5%. To the best of our knowledge, this is the first example of the production of highly fertile F1 hybrids between durum wheat and Ae. tauschii. The F1 and F2 hybrids are both similar morphologically to bread wheat and have vigorous growth habits. Cytological analyses of F1 male gametogenesis showed that meiotic restitution is responsible for the high fertility of the triploid F1 hybrids. The implications of these findings for the origin of bread wheat are discussed. 相似文献
7.
Alien cytoplasms cause a wide range of phenotypic alterations in the nucleus-cytoplasm (NC) hybrids in the Triticeae. Nuclear genomes of timopheevii wheat (Triticum timopheevii and Triticum araraticum) are fully compatible with the cytoplasm of Aegilops squarrosa, while those of a majority of emmer or durum wheat cultivars and more than half the wild emmer wheats are incompatible, and a maternal 1D chromosome is required to restore seed viability and male fertility in the NC hybrids. A euploid NC hybrid of Triticum durum cv. Langdon with Ae. squarrosa cytoplasm produced by introgressing the NC compatibility (Ncc) gene from T. timopheevii was used to identify random amplified polymorphic DNA (RAPD) markers linked to it. After a survey of 200 random decamer primers, four markers were selected, all of which were completely linked in 64 individuals of a SB8 mapping population. One marker was derived from a single locus, while three others were from interspersed repetitive sequences. Also, the hybrid chromosomes and those of the parental T. durum had identical C-banding patterns. RAPD-PCR analysis of 65 accessions from wild and cultivated tetraploid wheat species showed the exclusive presence of the markers in timopheevii wheat. In conclusion, the chromosomal region flanking Ncc of T. timopheevii is highly conserved in the genome of this group of tetraploid wheats. 相似文献
8.
M. Bernard S. Bernard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(4):390-399
Summary Two F5 strains of tetraploid triticale (2n= 4x=28), obtained from 6x triticaleX2 rye progenies, were crossed with diploid and tetraploid rye, some durum and bread wheats, and various 8x and 6x triticale lines. Meiosis in the different hybrid combinations was studied. The results showed that the haploid complement of these triticales consists of seven chromosomes from rye and seven chromosomes from wheat. High frequencies of PMCs showing trivalents were observed in hybrids involving the reference genotypes of wheat and triticale. These findings proved that several chromosomes from the wheat component have chromosome segments coming from two parental wheat chromosomes. The origin of these heterogeneous chromosomes probably lies in homoeologous pairing occurring at meiosis in the 6x triticaleX2x rye hybrids from which 4x triticale lines were isolated. A comparison among different hybrids combinations indicated that the involvement of D-genome chromosomes in homoeologous pairing is quite limited. In contrast, meiotic patterns in 4x triticale X 2x rye hybrids showed a quite high pairing frequency between some R chromosomes and their A and B homoeologues. 相似文献
9.
Vijay K Tiwari Nidhi Rawat Kumari Neelam Gursharn S Randhawa Kuldeep Singh Parveen Chhuneja Harcharan S Dhaliwal 《Génome》2008,51(9):757-766
Four different interspecific hybrids involving three different accessions of Aegilops longissima Schweinf. & Muschl. with high grain iron and zinc content and three Triticum turgidum L. subsp. durum (Desf.) Husn. cultivars with low micronutrient content were made for durum wheat biofortification and investigated for chromosome pairing, fertility, putative amphiploidy, and micronutrient content. The chromosome pairing in the 21-chromosome F1 hybrids (ABSl) consisted of 0-6 rod bivalents and occasionally 1 trivalent. All the F1 hybrids, however, unexpectedly showed partial but variable fertility. The detailed meiotic investigation indicated the simultaneous occurrence of two types of aberrant meiotic divisions, namely first-division restitution and single-division meiosis, leading to regular dyads and unreduced gamete formation and fertility. The F2 seeds, being putative amphiploids (AABBSlSl), had nearly double the chromosome number (40-42) and regular meiosis and fertility. The F1 hybrids were intermediate between the two parents for different morphological traits. The putative amphiploids with bold seed size had higher grain ash content and ash iron and zinc content than durum wheat cultivars, suggesting that Ae. longissima possesses a better genetic system(s) for uptake and seed sequestration of iron and zinc, which could be transferred to elite durum and bread wheat cultivars and exploited. 相似文献
10.
Two nuclear genes, vitality (Vi) on an A- or B-genome chromosome and species cytoplasm specific (scs) on a 1DL telosome from Triticum aestivum L. or a telosome from Aegilops uniaristata Vis. (un telosome), improved compatibility between the nucleus of Triticum turgidum L. var. durum and the cytoplasm of Ae. longissima S. &M. or Ae. uniaristata. To study interactions between Vi and scs and to determine the chromosomal location of Vi, 29-chromosome fertile plants were crossed with 13 D-genome disomic-substitution (d-sub) lines [except 5D(5A)] of 'Langdon' durum. F1 and backcross progenies were examined for meiotic chromosome number and pairing, fertility, and plant vigor. In 11 crosses, Vi restored seed viability but produced double-monosomics (d-monos) with greatly reduced growth and vigor. In contrast, crosses involving 1D(1A) and 1D(1B) d-sub lines produced d-monos with normal vigor and anthesis but nonfunctional pollen. A backcross of 1D + 1A d-mono F1 and 1D(1A) d-sub lines produced 11 male steriles; 3 had 13 II + 1 II 1D + 1 I 1A, 2 had 13 II + 2 I, 1 had 13 II + 1 II 1D(1A), and 5 were not examined. Crosses of 1D + 1A d-mono F1 with control durum, lo durum (with 1DL), and un durum (with un telosome) lines produced 16 male-sterile d-monos and 14 fertiles with 14 II + 1 I 1D, showing that 15-chromosome female gametes transmitted monosomes 1A and 1D. However, BC2F1's from 1D + 1B d-mono x fertile line with un telosome included 20 male-sterile d-monos, 6 fertile triple monosomics (13 II + 1 I 1D + 1 I 1B + t I un telosome), and 1 fertile plant with a 1B/1D translocation. Unlike d-mono 1A + 1D, d-mono 1B + 1D did not transmit 15-chromosome female gametes with monosomes 1D and 1B. Additional backcrosses also indicated that homozygous scs caused male sterility in 1D(1A) and 1D(1B) d-subs and that the procedure used was not suitable for the chromosomal location of Vi. 相似文献
11.
普通小麦与瓦维洛夫山羊草属间杂种的产生及其育性的细胞学 … 总被引:5,自引:1,他引:4
通过重复授粉和75μg.g^-1GA3处理,成功地获得了普通小麦(T.aestium)与瓦维洛夫山羊草(Ae.vavilovii)RM0286,RM0291 2个组合属间杂种的产生及其育性,期间表现出较高的可酱性,杂匀结实率分别39.84%和41.22%,杂种F1植株具有双亲的形态特征,并表现出强的生活力。通过对F1植株小孢子发生和花粉发生和花粉发育的细胞学观察发现,花粉母细胞减数分裂过程异常紊乱 相似文献
12.
The phenotypic manifestation and genetic control of embryo lethality observed in crosses between common wheat and rye were studied. It was found that crosses between common wheat and inbred self-fertile rye lines L2 and 535 gave rise to ungerminating grains, in which the development and differentiation of the hybrid embryo are arrested. Study of the degree of embryo development in the hybrid grains obtained by crossing common wheat varieties with inbred rye lines L2 and 535 showed that genotypes of the parents affected the ratio between undifferentiated embryos of various sizes. Analysis of this trait was performed by test crosses according to a novel pedigree program with the use of interlinear hybrids and a set of fourth-generation hybrid recombinant inbred lines. Rye line L2 was shown to bear the Eml (Embryo lethality) gene, which terminates the development of the hybrid embryo in amphihaploids. The suggestion of complementary interaction between wheat and rye genes during formation of a "n ew" character in wheat-rye F1 hybrids is discussed. A method of detecting an allele not complementary to the rye Eml allele in wheat is proposed. The proposed test program allows appropriate study of the system of wheat and rye genes involved in complementary interaction in the genotype of a distant hybrid. 相似文献
13.
Esterase isozyme zymograms of endosperms at milk stage were analyzed by polyacrylamide vertical slab gel electrophoresis. It was shown that Aegilops squarrosa, Ae. ventricosa and Ae. crassa with D genomes all had E1-1 band with the same migration rate. In zymograms, the two bands E1-1, E1-2 of common wheat (AABBDD) and emmer wheat (AABB) were actually each compossed of two closely adjacent bands. Zone E3 of the two species had five bands each. There were no difference in migration rate of these bands between the two species, but the activities of these enzymes were different and they showed regular change. Zymograms of the hybrids of reciprocal crosses between emmer and common wheat are mainly tended to maternal types and it is representing the dosage effect of D genome. 相似文献
14.
Highly fertile F1 hybrids were made between Triticum turgidum L. ssp. turgidum (2n = 28, AABB) and Aegilops tauschii Coss. (2n = 14, DD) without embryo rescue and hormone treatment. The F1 plants had an average seed set of 25%. Approximately 96% of the F2 seeds were able to germinate normally and about 67% of the F2 plants were spontaneous amphidiploid (2n = 42, AABBDD). Cytological analysis of male gametogenesis of the F1 plants showed that meiotic restitution is responsible for the high fertility. A mitosis-like meiosis led to meiotic restitution
at either of the two meiotic divisions resulting in unreduced gametes. Test crosses of the T. t. turgidum–Ae. tauschii amphidiploid with Ae. variabilis and rye suggested that the mitosis-like meiosis is controlled by one or more nuclear genes that continue to function in derived
lines. This discovery indicates a potential application of such genes in producing double haploids. 相似文献
15.
Steven S. Xu C. G. Chu S. Chao D. L. Klindworth J. D. Faris E. M. Elias 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(8):1575-1585
The durum wheat cultivar ‘Golden Ball’ (GB) is a source of resistance to wheat sawfly due to its superior solid stem. In the
late 1980s, Dr. Leonard Joppa developed a complete set of 14 ‘Langdon’ (LDN)–GB disomic substitution (DS) lines by using GB
as the chromosome donor and LDN as the recipient. However, these substitution lines have not been previously characterized
and reported in the literature. The objectives of this study were to confirm the authenticity of the substituted chromosomes
and to analyze the genetic background of the 14 LDN–GB DS lines with the aid of molecular markers, and to further use the
substitution lines for chromosomal localization of DNA markers and genes conferring the superior stem solidness in GB. Results
from simple sequence repeat marker analysis validated the authenticity of the substituted chromosomes in 14 LDN–GB DS lines.
Genome-wide scans using the target region amplification polymorphism (TRAP) marker system produced a total of 359 polymorphic
fragments that were used to compare the genetic background of substitution lines with that of LDN. Among the polymorphic TRAP
markers, 134 (37.3%) and 185 (51.5%) were present in LDN and GB, respectively, with only 10 (2.8%) derived from Chinese Spring.
Therefore, marker analysis demonstrated that each LDN–GB DS line had a pair of chromosomes from GB with a genetic background
similar to that of LDN. Of the TRAP markers generated in this study, 200 were successfully assigned to specific chromosomes
based on their presence or absence in the corresponding LDN–GB DS lines. Also, evaluation of stem solidness in the substitution
lines verified the presence of a major gene for stem solidness in chromosome 3B. Results from this research provides useful
information for the utilization of GB and LDN–GB DS lines for genetic and genomic studies in tetraploid wheat and for the
improvement of stem solidness in both durum and bread wheat. 相似文献
16.
17.
普通小麦×根茎冰草×黑麦三属杂种自交可育性的细胞学机理 总被引:1,自引:0,他引:1
普通小麦(TriticumaetivumL.,2n=6x=42;AABBDD)和根茎冰草(AgropyronmichnoiReshev.,2n=4x=28;PPPP)间的F1杂种(2n=5x=35:ABDPP)与两个黑麦(SecalecerealeL.,2n=2x=14;RR)品种杂交,产生了自交可育的三属杂种。经细胞学研究表明,这种自交可育性是由于在某些细胞中通过两种方式发生了第一次减数分裂的失败,即单价染色体在赤道板发生分裂和单价染色体在一极的聚集,从而异致了有功能的雌、雄配子的形成。有功能配子的形成受染色体配对频率、基因型和环境状况的影响。冰草属的P染色体组存在染色体分离的控制基因,从而引起含有冰草属的杂种能够形成有功能的配子且自交可育。 相似文献
18.
Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH 总被引:3,自引:0,他引:3
K.-B. Lim M. S. Ramanna J. H. de Jong E. Jacobsen J. M. van Tuyl 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):219-230
A detailed analysis of microsporogenesis was carried out in three diploid lily cultivars (2n=2x=24) and three diploid interspecific
hybrids (2n=2x=24) using DNA in situ hybridisation methods (GISH and FISH). In cvs. Gelria (Lilium longiflorum; L genome), Connecticut King and Mont Blanc (both Asiatic hybrids; Agenome) meiosis was regular and only haploid gametes were formed while
the three interspecific hybrids between L. longiflorum×Asiatic hybrid (LA) showed a variable frequency of meiotic nuclear restitution and stainable 2n-pollen formation ranging
from 3% to 30%. An analysis of meiotic chromosome behaviour of the LA hybrids through GISH and FISH revealed that: (1) the
parental chromosomes could be clearly discriminated into univalents, half-bivalents and bivalents in the PMCs; (2) in some
of the PMCs the entire complement was present either as univalents or half-bivalents which had the potential to divide equationally
(following centromere division) during the first division leading to first division restitution (FDR) gametes; (3) more frequently,
however, in one and the same PMC the univalents and half-bivalents divided equationally whereas the bivalents disjoined reductionally
at the same time giving rise to 2n-gametes that could vary from the well-known FDR or SDR 2n-gametes. We indicate this novel
type of restitution mechanism as Indeterminate Meiotic Restitution (IMR). In order to confirm the occurrence of IMR gametes,
the chromosome constitutions of eight triploid BC1 progenies derived from backcrossing the 2n-gamete producing the LAhybrids to the Asiatic hybrid parents were analysed through
in situ hybridisation. The results indicated that there were seven BC1 plants in which FDR 2n-gametes, with or without homoeologous recombinations, were functional, whereas in one case the 2n-gamete
resulting from IMR was functional. In the latter, there was evidence for the occurrence of genetic recombination through homoeologous
crossing-over as well as through the assortment of homoeologous chromosomes. A singular feature of the IMR 2n-gamete was that
although it transmitted a euploid number of 24 chromosomes to the BC1 progeny, the number of chromosomes transmitted from the two parental species was dissimilar: 9 L-genome chromosomes and 15
A-genome chromosomes instead of 12 of each.
Received: 15 May 2000 / Accepted: 4 December 2000 相似文献
19.
Cytological evidences of SDR-FDR mixture in the formation of 2n eggs in a potato diploid clone 总被引:2,自引:0,他引:2
C. Conicella A. Barone A. Del Giudice L. Frusciante L. M. Monti 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,81(1):59-63
Summary Macrosporogenesis and microsporogenesis were investigated in a diploid S. tuberosum x S. chacoense potato hybrid, characterized by more than 50% 2n egg formation. Fifty-five percent of dyad formation of 2n macrospores is ascribed to two meiotic abnormalities: omission of the second meiotic division, occurring at a frequency of 38%, and irregular spindle axis orientation at metaphase I at a frequency of 16%: These abnormalities give origin to a mixture of 2n eggs, composed of mostly second division restitution (SDR) and a small portion of first division restitution (FDR). Microsporogenesis showed rare dyads of 2n microspores depending on parallel spindles observed in anaphase II.Contribution no. 53 from the Center of Vegetable Breeding, CNR, Portici, Italy 相似文献
20.
山羊草属异源多倍体植物基因组进化的RAPD分析 总被引:5,自引:0,他引:5
和24个随机引物对山羊草属(Aegilops L.)异源多倍体物种对其祖先二倍体物进行RAPD分析,对扩增出的313条带进行聚类分析发现,含D基因组的多倍体与二倍体祖先Ae.squarrosa(DD)在聚类图上聚为一支;除Ae.juvenalis(DDMMUU)聚到上一支外,含U基因组的多倍 与二倍体祖先Ae.umbellulata(UU)在聚类图上聚为另一支;多倍体与其他二倍体均不聚在一起,表明多倍体分别与Ae.squarrosa(DD)、Ae.umbellulata(UU)具有较近的亲缘关系,这说明多倍体开之后,D和U基因组变化较小,而其他基因组则发生了较大的变化。 相似文献