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1.
Hayashida-Soiza G Uchida A Mori N Kuwahara Y Ishida Y 《Journal of applied microbiology》2008,105(5):1672-1677
Aims: To purify and characterize compounds with antimicrobial activity from Pseudoalteromonas haloplanktis inhibition (INH) strain. Methods and Results: The P. haloplanktis isolated from a scallop hatchery was used to analyse antibacterial activities. Crude extracts were obtained with ethyl acetate of the cultured broth, after separation of bacterial cells, and assays against six strains of marine bacteria and nine clinically important pathogenic bacteria. The active compounds were purified from ethyl acetate extracts, by a combination of SiO2 column and thin layer chromatography. Two active fractions were isolated, and chemical structures of two products from the major one were unambiguously identified as isovaleric acid (3-methylbutanoic acid) and 2-methylbutyric acid (2-methylbutanoic acid), by comparing their mass spectra and 1H- and 13C-nuclear magnetic resonance spectra to those of authentic compounds. Conclusions: In the antibacterial activity of P. haloplanktis INH strain, extra cell compounds are involucred, mainly isovaleric and 2-methylbutyric acids. Significance and Impact of the Study: Production of antimicrobial compounds by marine micro-organisms has been widely reported; however, the efforts not always are conducted to purification and applications of these active compounds. This study is a significant contribution to the knowledge of compounds unique from marine bacteria as potential sources of new drugs in the pharmacological industry. 相似文献
2.
L P Terekhova O A Galatenko V V Kuliaeva I V Tolstykh T P Golova G S Katrukha N E Zhukhmistova O L Ol'khovatova N D Malkina Iu V Bo?kova 《Antibiotiki i khimioterapii͡a》1992,37(12):19-21
An actinomycete strain designated as 4297 was isolated from a soil sample collected near Moscow. The strain produced a complex of two antibiotics. One of them had a broad antibacterial spectrum and, in terms of its physicochemical properties and X-ray structural evidence, was identified with griseoviridin. The other was active against gram-positive bacteria and, by its chromatographic comparison with an authentic sample, mass spectroscopic determination of the molecular weight and UV spectra, was identified with etamycin. The strain 4297 differed from the described cultures producing griseoviridin and etamycin. By the taxonomic features it was classified as belonging to Streptomyces albolongus. 相似文献
3.
O'Halloran JA Barbosa TM Morrissey JP Kennedy J O'Gara F Dobson AD 《Journal of applied microbiology》2011,110(6):1495-1508
Aims: To evaluate the diversity and antimicrobial activity present among Pseudovibrio spp. isolated from marine sponges. Methods and Results: Seventy‐three bacterial isolates from the marine sponges Polymastia boletiformis, Axinella dissimilis and Haliclona simulans were identified as Pseudovibrio spp. using phylogenetic analysis of 16S rRNA gene sequences. Genetic diversity among these isolates was estimated using random amplification of polymorphic DNA (RAPD), and 33 RAPD types were identified among the 73 Pseudovibrio isolates. These Pseudovibrio spp. were assayed for the production of compounds with antimicrobial activity against various clinically relevant pathogens. Sixty‐two (85%) of the isolates showed activity against at least one of the pathogens tested, including Escherichia coli, Salmonella enterica serotype Typhimurium, methicillin‐resistant Staphylococcus aureus (MRSA), and Clostridium difficile. PCR screens of the Pseudovibrio isolates also revealed the presence of potential antibiotic‐producing polyketide synthase genes. Conclusions: Marine sponges harbour a diverse population of Pseudovibrio spp., the majority of which demonstrate antimicrobial activity. The identification of several different antimicrobial activity spectra suggests that the Pseudovibrio isolates may produce a suite of antimicrobial compounds. Significance and Impact of the Study: This is the first study in which an extended population of Pseudovibrio isolates from marine sponges has been analysed and establishes the little‐studied Pseudovibrio as a potentially important genus in the search for antimicrobial compounds of clinical relevance. 相似文献
4.
A Bacillus sp. strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16S rDNA sequencing. The
phylogenetic analysis indicated that this strain has low sequence similarity with most Bacillus spp., suggesting a new species was isolated. The antimicrobial activity was detected starting at the exponential growth phase,
and maximum activity was observed at stationary phase. The substance was inhibitory to a broad range of indicator strains,
incluing pathogenic and food spoilage bacteria such as Listeria monocytogenes, B. cereus, Aeromonas hydrophila, Erwinia carotovora, Pasteurella haemolytica, Salmonella Gallinarum, among other. The antibacterial substance was stable over a wide pH range, but it was sensitive to pronase E and lipase.
The antibacterial substance was bactericidal and bacteriolytic to L. monocytogenes and B. cereus at 160 AU ml−1. The identification of a broad range bacteriocin-like inhibitory substance active against L. monocytogenes addresses an important aspect of food protection against pathogens and spoilage microorganisms. 相似文献
5.
On the basis of the one strain–many compounds strategy, five compounds including two new holomycin derivatives 2 – 3 , two new cyclopropaneacetic acid derivatives 4 – 5 , together with one known compound holomycin ( 1 ) were isolated from a marine‐derived bacterium Streptomyces sp. DT‐A37. Their structures were elucidated using NMR and HR‐ESI‐MS analyses. All these compounds were evaluated for their antimicrobial activity, cytotoxic activity, and inhibitory activity against BRD4 protein. Compound 1 exhibited potent cytotoxicity against H1975 cells with IC50 value of 1 μm , and its minimal inhibitory concentration values against Escherichia coli and Staphylococcus aureus were both 64 μm . 相似文献
6.
Boudjella H Bouti K Zitouni A Mathieu F Lebrihi A Sabaou N 《Microbiological research》2006,161(4):288-298
A new actinomycete strain designated Sg 10, producing antimicrobial substances was isolated from an Algerian soil. Morphological and chemical studies indicated that strain Sg 10 belonged to the genus Streptosporangium. The comparison of its physiological characteristics with those of known species of Streptosporangium showed significant differences with the nearest species Streptosporangium carneum. Analysis of the 16S rDNA sequence of strain Sg 10 showed a similarity level ranging between 96.3% and 97.8% within Streptosporangium species, with S. carneum the most closely related. However, the phylogenetic analysis indicated that strain Sg 10 represent a distinct phyletic line suggesting a new genomic species. The antimicrobial activity of strain Sg 10 showed an antibacterial activity against Gram-positive bacteria as well as an antifungal one. Four active products were isolated from the culture broth using various separation procedures. On the basis of UV-VIS spectrometry, infrared spectroscopy and chemical revelations, the antibiotics were classified in the group of glycosylated aromatics. 相似文献
7.
本文旨在分离具有纤溶活性作用的化合物和鉴定产生纤溶活性化合物的菌株FG216的种属分类。以马铃薯蔗糖培养基为种子培养基,改良查氏培养基为发酵培养基对菌株进行发酵培养,用甲醇作为提取溶剂,通过半制备型高效液相色谱从真菌FG216的1 L发酵液中分离和精制了12 mg纤溶活性化合物,该纤溶活性化合物在纤溶酶原和单链尿激酶性纤溶酶原激活剂相互活化反应体系中添加10μg/mL活性最高。对FG216菌株rDNA的ITS基因(ITS-5.8 S rDNA)进行PCR扩增、测序,从GenBank获取相似序列,通过序列比对分析和系统发育分析表明菌株FG216与Stachybotrys longispora同源性最高。从分离的海洋微生物葡萄穗霉属菌株FG216分离得到的纤溶活性化合物具体促进纤溶酶原和单链尿激酶性纤溶酶原激活剂相互活化的作用。 相似文献
8.
Although comparatively little research has been undertaken into the secondary metabolites of bryozoans as compared with those of other marine invertebrates, bryozoans have proven to be an excellent source of novel and/or biologically active compounds. The majority of bryozoan metabolites isolated to date have been alkaloids. In our continuing search for bioactive and/or novel compounds from New Zealand marine bryozoans, we undertook an investigation of an extract of Pterocella vesiculosa (order Cheilostomatida, suborder Ascophorina, family Catenicellidae) which possessed activity against P388 murine leukaemia cells. Two alkaloids, pterocellins A–B (1–2) have been isolated from the bryozoan. The biological activity of these alkaloids was examined including their activities in the in vitro 60 cell line panel and in vivo hollow fibre assays at the National Cancer Institute (NCI). The isolation and characterisation of further pterocellin analogues is currently in progress and tentative structures for two new members of this series, pterocellins C–D (3–4) are proposed, based on NMR and mass spectral data. 相似文献
9.
Rokuro Masuma Yuichi Yamaguchi Masanobu Noumi Satoshi Ōmura Michio Namikoshi 《Mycoscience》2001,42(5):455-459
We studied the effect of sea water concentration in a culture medium on fungal growth and the production of antimicrobial
metabolites. Most of the marine fungal isolates were identified as members of the same genera as terrestrial isolates, such
asAspergillus andTrichoderma. Many of the marine fungi isolated grew more abundantly as the sea water concentration increased. The production of antimicrobial
materials was improved as the sea water concentration increased. Even though the marine fungi were considered to be similar
to fungi from terrestrial environments, from a mycological perspective, the two types have different physiological characteristics.
The fungi from marine samples are useful microbial resources in the search for new bioactive compounds. 相似文献
10.
Joseph Selvin S. Shanmughapriya R. Gandhimathi G. Seghal Kiran T. Rajeetha Ravji K. Natarajaseenivasan T. A. Hema 《Applied microbiology and biotechnology》2009,83(3):435-445
The sponge-associated actinomycetes were isolated from the marine sponge Dendrilla nigra, collected from the southwest coast of India. Eleven actinomycetes were isolated depending upon the heterogeneity and stability
in subculturing. Among these, Nocardiopsis dassonvillei MAD08 showed 100% activity against the multidrug resistant pathogens tested. The culture conditions of N. dassonvillei MAD08 was optimized under submerged fermentation conditions for enhanced antimicrobial production. The unique feature of
MAD08 includes extracellular amylase, cellulase, lipase, and protease production. These enzymes ultimately increase the scope
of optimization using broad range of raw materials which might be efficiently utilized. The extraction of the cell free supernatant
with ethyl acetate yielded bioactive crude extract that displayed activity against a panel of pathogens tested. Analysis of
the active thin layer chromatography fraction by Fourier transform infrared and gas chromatography-mass spectrometry evidenced
11 compounds with antimicrobial activity. The ammonium sulfate precipitation of the culture supernatant at 80% saturation
yielded an anticandidal protein of molecular weight 87.12 kDa. This is the first strain that produces both organic solvent
and water soluble antimicrobial compounds. The active extract was non-hemolytic and showed surface active property envisaging
its probable role in inhibiting the attachment of pathogens to host tissues, thus, blocking host–pathogen interaction at an
earlier stage of pathogenesis. 相似文献
11.
Meyyappan Arumugam Anindita Mitra Parasuraman Jaisankar Shreya Dasgupta Tuhinadri Sen Ratan Gachhui Ujjal Kumar Mukhopadhyay Joydeep Mukherjee 《Applied microbiology and biotechnology》2010,86(1):109-117
A marine actinobacterium isolated from the Bay of Bengal, India and previously found to be producing an antimicrobial and
cytotoxic terpenoid was further investigated for antimicrobial metabolites. The bacterium was preliminarily identified as
a new species of the genus Streptomyces (strain MS1/7). The cell-free culture broth was extracted with n-butanol and purified using silica gel column chromatography and high-performance liquid chromatography. Molecular characterization
was done using ESI mass, IR and 1H and 13C NMR spectrometry. 2-Allyloxyphenol (MW 150; C9H10O2), a synthetic drug and chemical intermediate, was obtained as a natural product for the first time. Serendipitous natural
occurrence provided new insights into the synthetic molecule. 2-Allyloxyphenol was found to be inhibitory to 21 bacteria and
three fungi in the minimum range 0.2–1.75 mg mL−1 determined by agar dilution method. 2-Allyoxyphenol possesses strong antioxidant property (IC50 22 μg mL−1, measured by 1, 1-diphenyl-2-picryl hydrazyl scavenging activity). Hydroxyl and allyloxy groups in 2-allyloxyphenol were
responsible for antimicrobial and antioxidant activities. 2-Allyloxyphenol has marked resemblance to smoky aroma and is two
to three times more active as an antimicrobial than some commercial smoke-flavour compounds. Absence of hemolytic toxicity,
potential carcinogenicity, cytotoxicity and reports of toxic reactions in literature suggest possible application of 2-allyloxyphenol
as a food preservative and an oral disinfectant. 相似文献
12.
从胶州湾附近海域水样中分离筛选到一株可产生天然蓝色素的链霉菌。采用通用引物16F27/16R1492扩增该菌株的16SrDNA,对测序结果进行序列分析,采用Neighbor-Joining(N-J)法构建系统发育进化树,同时结合链霉菌的传统形态学和生理生化特性对菌株进行鉴定。16SrDNA序列分析表明菌株与Streptomyces violaceolatus KCTC 9772相近,相似率为99%。在Neighbour-Joining法构建的系统发育进化树中,该菌株与Streptomyces violaceolatus聚类在同一分枝上。因此将该菌株鉴定为紫边链霉菌Streptomyces violaceolatus。 相似文献
13.
Zhen‐Yu Li Yu‐Cheng Gu Dianne Irwin Jacqueline Sheridan John Clough Ping Chen Shu‐Ying Peng Yi‐Ming Yang Yue‐Wei Guo 《化学与生物多样性》2009,6(10):1744-1750
Two new Daphniphyllum alkaloids, macropodumines J and K ( 1 and 2 , resp.), together with six known structurally related alkaloids, 3 – 8 , were isolated from the bark of Daphniphyllum macropodum Miq . The structures of the new compounds 1 and 2 were elucidated on the basis of a comprehensive analysis of their spectroscopic and chemical data. Macropodumine J ( 1 ) contains a CN group which is relatively rare in naturally occurring alkaloids. All isolated compounds were tested for their insecticidal activities against a number of insect species. Daphtenidine C ( 5 ) is the most active compound against Plutella xylostella. This is the first report of insecticidal properties of Daphniphyllum alkaloids. 相似文献
14.
Osama Mostafa Mohammed Al-Shehri Mahmoud Moustafa 《Saudi Journal of Biological Sciences》2022,29(1):217-227
Parasitic diseases especially those prevail in tropical and subtropical regions severely threaten the lives of people due to available drugs found to be ineffective as several resistant strains have been emerged. Due to the complexity of the marine environment, researchers considered it as a new field to search for compounds with therapeutic efficacy, marine sponges represents the milestone in the discovery of unique compounds of potent activities against parasitic infections. In the present article, literatures published from 2010 until March 2021 were screened to review antiparasitic potency of bioactive compounds extracted from marine sponges. 45 different genera of sponges have been studied for their antiparasitic activities. The antiparasitic activity of the crude extract or the compounds that have been isolated from marine sponges were assayed in vitro against Plasmodium falciparum, P. berghei, Trypanosoma brucei rhodesiense, T. b. brucei, T. cruzi, Leishmania donovani, L. tropica, L. infantum, L. amazonesis, L. major, L. panamesis, Haemonchus contortus and Schistosoma mansoni. The majority of antiparastic compounds extracted from marine sponges were related to alkaloids and peroxides represent the second important group of antiparasitic compounds extracted from sponges followed by terpenoids. Some substances have been extracted and used as antiparasitic agents to a lesser extent like steroids, amino acids, lipids, polysaccharides and isonitriles. The activities of these isolated compounds against parasites were screened using in vitro techniques. Compounds' potent activity in screened papers was classified in three categories according to IC50: low active or inactive, moderately active and good potent active. 相似文献
15.
Qian Sun Yun‐Heng Shen Jun‐Mian Tian Jian Tang Juan Su Run‐Hui Liu Hui‐Liang Li Xi‐Ke Xu Wei‐Dong Zhang 《化学与生物多样性》2009,6(10):1751-1757
A phytochemical investigation of the bulbs of Crinum asiaticum L. var. sinicum Baker resulted in the isolation of two new alkaloids, asiaticumines A and B ( 1 and 2 , resp.), together with 21 known compounds, including nine alkaloids, four amides, five phenolic compounds, and three flavonoids. All 23 compounds were isolated for the first time from Crinum asiaticum L. var. sinicum Baker . Their structures were elucidated by spectroscopic methods. In addition, ten alkaloids, 1 – 10 , were evaluated for their cytotoxic activities against human tumor cell lines A549, LOVO, HL‐60, and 6T‐CEM. Compounds 3, 4 , and 7 – 10 selectively showed remarkable inhibition against one or more of the tested cell lines. 相似文献
16.
Nonribosomal peptide synthetase (NRPS) adenylation (A) domain genes were investigated by polymerase chain reaction for 109
bacteria isolated from four South China Sea sponges, Stelletta tenuis, Halichondria rugosa, Dysidea avara, and Craniella australiensis. Meanwhile, the antimicrobial bioassay of bacteria with NRPS genes were carried out to confirm the screening of NRPS genes.
Fifteen bacteria were found to contain NRPS genes and grouped into two phyla Firmicutes (13 of 15) and Proteobacteria (two
of 15) according to 16S rDNA sequences. Based on the phylogenetic analysis of the conserved A domain amino acid sequences,
most of the NRPS fragments (11 of 15) showed below 70% similarity to their closest relatives suggesting the novelty of these
NRPS genes. All of the 15 bacteria with NRPS genes have antimicrobial activities, with most of them exhibiting activity against
multiple indicators including fungi and gram-positive and gram-negative bacteria. The different antimicrobial spectra indicate
the chemical diversity of biologically active metabolites of sponge-associated bacteria and the possible role of bacterial
symbionts in the host’s antimicrobial chemical defense. Phylogenetic analysis based on the representative NRPS genes shows
high diversity of marine NRPS genes. The combined molecular technique and bioassay strategy will be useful to obtain sponge-associated
bacteria with the potential to synthesize bioactive compounds.
An erratum to this article can be found at 相似文献
17.
Data on the chemical structures and biologic activities of metabolites of obligate and facultative marine actinobacteria published between 2000 and 2007 are reviewed. The structural features of five groups of metabolites related to macrolides and compounds containing lactone, quinone, and diketopiperazine residues; cyclic peptides; alkaloids; and compounds of combined nature are discussed. The review shows the large chemical diversity of metabolites of actinobacteria isolated from marine ecotopes. In addition to metabolites identical to those previously isolated from terrestrial actinobacteria, marine actinobacteria produce compounds not found in other natural sources, including microorganisms. Probably, the biosynthesis of new chemotypes of bioactive compounds by marine actinobacteria is related to the chemical adaptation of microorganisms to the marine environment. The review emphasizes the importance of chemical studies of metabolites produced by marine actinobacteria. These studies will provide new data on marine microbial producers of biologically active compounds and the chemical structures and biologic activities of new natural lowmolecular-weight bioregulators. 相似文献
18.
《Phytomedicine》2015,22(1):183-202
The present review describes research on novel natural isoquinoline alkaloids and their N-oxides isolated from different plant species. More than 200 biological active compounds have shown confirmed antimicrobial, antibacterial, antitumor, and other activities. The structures, origins, and reported biological activities of a selection of isoquinoline N-oxides alkaloids are reviewed. With the computer program PASS some additional SAR (structure–activity relationship) activities are also predicted, which point toward new possible applications of these compounds. This review emphasizes the role of isoquinoline N-oxides alkaloids as an important source of leads for drug discovery. 相似文献
19.
T. V. Antipova V. P. Zhelifonova B. P. Baskunov S. M. Ozerskaya N. E. Ivanushkina A. G. Kozlovsky 《Applied Biochemistry and Microbiology》2011,47(3):288-292
Screening of producers of secondary metabolites was carried out among 25 fungal strains of Penicillium genus isolated from permafrost in Arctic and Antarctic regions and Kamchatka. Nearly 50% of the investigated strains synthesize
biologically active substances of alkaloid nature: ergot alkaloids, diketopiperazinees, and quinoline derivatives. A large
group of the identified metabolites belongs to mycotoxins. A strain of Penicillium waksmanii was found producing epoxyagroclavine-I and quinocitrinines. The main physiological and biochemical characteristics of this
producer were investigated. 相似文献
20.
G. Sathiyanarayanan R. Gandhimathi B. Sabarathnam G. Seghal Kiran Joseph Selvin 《Bioprocess and biosystems engineering》2014,37(3):561-573
Twenty-nine actinobacterial strains were isolated from marine sponge Spongia officinalis and screened for antagonistic activity against various bacterial and fungal pathogens. The active antibiotic producer MAPS15 was identified as Streptomyces sp. using 16S rRNA phylogenetic analysis. The critical control factors were selected from Plackett–Burman (PB) factorial design and the bioprocess medium was optimized by central composite design (CCD) for the production of bioactive metabolite from Streptomyces sp. MAPS15. The maximum biomass and active compound production obtained with optimized medium was 6.13 g/L and 62.41 mg/L, respectively. The economical carbon source, paddy straw was applied for the enhanced production of bioactive compound. The purified active fraction was characterized and predicted as pyrrolidone derivative which showed broad spectrum of bioactivity towards indicator organisms. The predicted antimicrobial spectra suggested that the Streptomyces sp. MAPS15 can produce a suite of novel antimicrobial drugs. 相似文献