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1.
Shi D Guo S Liao S Su R Guo M Liu N Li P Tang Z 《Biological trace element research》2012,145(3):312-317
To investigate the protection of selenium on hepatic mitochondrial functions, 90 7-day-old ducklings were randomly divided
into three groups (groups I–III). Group I was used as a blank control. Group II was administered with aflatoxin B1 (0.1 mg/kg body weight). Group III was administered with aflatoxin B1 (0.1 mg/kg body weight) plus selenium (sodium selenite, 1 mg/kg body weight). All treatments were given once daily for 21 days.
The results showed that the activities of hepatic mitochondrial complexes I–IV in group II ducklings significantly decreased
when compared with group I (P < 0.01). Furthermore, the activities of hepatic mitochondrial complexes I–IV in group III significantly increased when compared
with group II (P < 0.05). The hepatic mitochondrial respiratory control ratio (RCR) in group II ducklings significantly decreased when compared
with group I (P < 0.01). In addition, the hepatic mitochondrial RCR in group III significantly increased when compared with group II (P < 0.05). These results revealed that the aflatoxin B1 significantly induced hepatic mitochondrial dysfunction in the activities of hepatic mitochondrial respiratory chain complexes
I–IV and the RCR in ducklings. However, sodium selenite could significantly ameliorate the negative effect induced by aflatoxin
B1. 相似文献
2.
To investigate the influence of selenium on body weights and the immune organ indexes in ducklings administrated with aflatoxin
B1 (AFB1), 90 7-day-old ducklings were randomly divided into three groups (groups I–III). Group I was used as a blank control. Group
II was administered with AFB1 (0.1 mg/kg body weight). Group III was administered with AFB1 (0.1 mg/kg body weight) plus sodium selenite (1 mg/kg body weight). All treatments were given once daily for 21 days. It
showed that the ducklings’ bursa of fabricius, thymus indexes, and body weights in group II significantly decreased when compared
with group I (P < 0.01). Furthermore, the spleen indexes significantly decreased (P < 0.01). However, the ducklings’ bursa of fabricius and thymus indexes, body weights in group III ducklings significantly
increased when compared with group II (P < 0.01). In addition, the spleen indexes significantly decreased (P < 0.01). These results revealed that AFB1 significantly affect ducklings’ growth and immune organs development. However, selenium significantly ameliorated the negative
effects induced by AFB1. 相似文献
3.
Wang Y 《Biological trace element research》2009,128(2):184-190
The present research evaluated differential effects of sodium selenite and nano-Se on growth performance, tissue Se distribution,
and glutathione peroxidase (GSH-Px) activity of avian broiler. Broilers were randomly segregated into 12 groups so that three
replicates were available for each of the three treatments (T-1, T-2, and T-3) and control groups. The control groups were
fed basal diets without Se addition. T-1, T-2, and T-3 were fed with diets containing 0.2 mg kg−1 sodium selenite, 0.2 mg kg−1 nano-Se, and 0.5 mg kg−1 nano-Se, respectively. Compared with the control, Se supplementation remarkably improved daily weight gain and survival rate
and decreased feed conversion ratio (P < 0.05). However, no significant difference was observed between T-1, T-2, and T-3. The tissue Se content was significantly
higher (P < 0.05) in Se-supplemented groups than the control, and T-3 showed the highest. Furthermore, higher Se content was observed
in liver, and there was a significant difference (P < 0.05) compared with that in muscle. As for serum and hepatic GSH-Px activities, Se supplementation remarkably improved
GSH-Px activity (P < 0.05), and there was no significant difference (P > 0.05) between treatments (T-1, T-2, and T-3). 相似文献
4.
Annadurai T Vigneshwari S Thirukumaran R Thomas PA Geraldine P 《Journal of physiology and biochemistry》2011,67(4):519-530
Acetyl-l-carnitine (ALCAR) has been shown to prevent experimental selenite cataractogenesis, a manifestation of oxidative stress,
but little is known about its potential in other settings of oxidative stress. The present study was based on the hypothesis
that ALCAR prevents carbon tetrachloride (CCl4)-induced oxidative stress in vital tissues. Male albino Wistar rats were divided into three groups, each of six rats. Group
I (control) rats received only vehicle (1 ml/kg b.w.) for 4 days; Group II (CCl4-exposed, untreated) rats received CCl4 (2 ml/kg b.w.) on the second and third days and vehicle on the first and fourth days; Group III (CCl4-exposed, ALCAR-treated) rats received ALCAR (200 mg/kg b.w.) for 4 days and CCl4 on the second and third days. All administrations were made intraperitoneally. After the experimental period, significantly
(P < 0.05) elevated mean serum levels of aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase
were observed in Group II rats when compared to Group I and Group III rats. The mean levels of vitamin C, vitamin E, and reduced
glutathione and the mean activities of superoxide dismutase, catalase, and glutathione peroxidase were significantly (P < 0.05) lower in samples of hemolysate and of liver, kidney, and brain tissues of Group II rats than those in Group I and
Group III rats. The mean level of lipid peroxidation was significantly (P < 0.05) higher in Group II rats than that in Group I and Group III rats. Moreover, the CCl4-induced upregulation of inducible nitric oxide synthase expression was prevented by ALCAR in the liver and brain tissues.
These results suggest that ALCAR is able to prevent the CCl4-induced oxidative stress. 相似文献
5.
The study was conducted to investigate the effects of dietary maternal selenomethionine or sodium selenite supplementation
on performance and selenium status of broiler breeders and their next generation. Two hundred and forty 39-week-old Lingnan
yellow broiler breeders were allocated randomly into two treatments, each of which included three replicates of 40 birds.
Pretreatment period was 2 weeks, and the experiment lasted 8 weeks. The groups were fed the same basal diet supplemented with
0.30 mg selenium/kg of sodium selenite or selenomethionine. After incubation, 180 chicks from the same parental treatment
group were randomly divided into three replicates, with 60 birds per replicate. All the offspring were fed the same diet containing
0.04 mg selenium/kg, and the experiment also lasted 8 weeks. Birth rate was greater (p < 0.05) in hens fed with selenomethionine than that in hens fed with sodium selenite. The selenium concentration in serum,
liver, kidney, and breast muscle of broiler breeders, selenium deposition in the yolk, and albumen and tissues' (liver, kidney,
breast muscle) selenium concentrations of 1-day-old chicks were significantly (p < 0.01) increased by maternal selenomethionine supplementation compared with maternal sodium selenite supplementation. The
antioxidant status of 1-day-old chicks was greatly improved by maternal selenomethionine intake in comparison with maternal
sodium selenite intake and was evidenced by the increased glutathione peroxidase activity in breast muscle (p < 0.05), superoxide dismutase activity in breast muscle and kidney (p < 0.05), glutathione concentration in kidney (p < 0.01), total antioxidant capability in breast muscle and liver (p < 0.05), and decreased malondialdehyde concentration in liver and pancreas (p < 0.05) of 1-day-old chicks. Feed utilization was better (p < 0.05), and mortality was lower (p < 0.05) in the progeny from hens fed with selenomethionine throughout the 8-week growing period compared with those from
hens fed with sodium selenite. In summary, we concluded that maternal selenomethionine supplementation increased birth rate
and Se deposition in serum and tissues of broiler breeders as well as in egg yolk and egg albumen more than maternal sodium
selenite supplementation. Furthermore, maternal selenomethionine intake was also superior to maternal sodium selenite intake
in improving the tissues Se deposition and antioxidant status of 1-day-old chicks and increasing the performance of the progeny
during 8 weeks of post-hatch life. 相似文献
6.
The present study was carried to evaluate the protective effects of melatonin alone and vitamin E with selenium combination
against high dose cadmium-induced oxidative stress in rats. The control group received subcutanous physiological saline. The
first study group administered cadmium chloride (CdCl2) by subcutaneous injection of dose of 1 mg/kg. The second study group administered cadmium plus vitamin E with selenium (1 mg/kg
sodium selenite with 60 mg/kg vitamin E); the third study group administered cadmium plus 10 mg/kg melatonin (MLT); the fourth
study group administered CdCl2 plus a combination of melatonin in addition to vitamin E and selenium for a month. Determination levels of plasma malondialdehyde
(MDA), glutathione peroxidase (GSH-Px), blood superoxide dismutase (SOD), creatinine alanine transaminase (ALT), aspartate
aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), and urea were measured in serum. In only CdCl2 administered group, the MDA, creatinine, ALT, AST, ALP, and urea levels in the serum were significantly higher than the control
group (p < 0.05). Whereas in all other groups, this values were significantly lower than the only CdCl2 administered group (p < 0.05). Erythrocytes GSH-Px, serum SOD activities of only CdCl2 received group were significantly lower than the control group (p < 0.05). In conclusion, vitamin E + Se, melatonin and vitamin E, and Se, in addition to MLT combinations, had protective
effects against high dose cadmium-induced oxidative damage. 相似文献
7.
Forty-eight 2-year-old Liaoning Cashmere goats (body weight = 38.0 ± 2.94 kg) were used to investigate the effects of dietary
iodine (I) and selenium (Se) supplementation on nutrient digestibility, serum thyroid hormones, and antioxidant status during
the cashmere telogen period to learn more about the effects of dietary I and Se on nutrition or health status of Cashmere
goats. The goats were equally divided into six groups of eight animals each that were treated with 0, 2, or 4 mg of supplemental
I/kg dry matter (DM) and 0 or 1 mg of supplemental Se/kg DM in a 2 × 3 factorial arrangement of treatments. The six treatments
were I0Se0, I2Se0, I4Se0, I0Se1, I2Se1, and I4Se1. The concentrations of I and Se in the basal diet were 0.67 and 0.09 mg/kg DM, respectively. The study started in March and
proceeded for 45 days. Supplemental I or Se alone had no effect on nutrient digestibility and nitrogen metabolism. However,
the interaction between I and Se was significant regarding the digestibility of acid detergent fiber (ADF; P < 0.05), and compared with group I4Se1, the digestibility of ADF was significantly increased in group I4Se0 (P < 0.05). Selenium supplementation did not affect serum triiodothyronine (T3) or thyroxine (T4) concentrations. However, the concentration of serum T4 but not that of T3 was significantly increased with I supplementation (P < 0.05). In addition, serum superoxide dismutase (SOD) activity was not affected (P > 0.05), but serum glutathione peroxidase (GSH-Px) activity was significantly decreased by I supplementation (P < 0.05). The antioxidant status was improved by Se supplementation, and the activities of SOD and GSH-Px were significantly
increased (P < 0.05). 相似文献
8.
Mustafa Akil Mursel Bicer Mehmet Kilic Mustafa Cihat Avunduk Rasim Mogulkoc Abdulkerim Kasim Baltaci 《Biological trace element research》2011,139(3):341-346
There are a few of studies examining how selenium, which is known to reduce oxidative damage in exercise, influences glucose
metabolism and exhaustion in physical activity. The present study aims to examine how selenium administration affects liver
glycogen levels in rats subjected to acute swimming exercise. The study included 32 Sprague–Dawley type male rats, which were
equally allocated to four groups: Group 1, general control; Group 2; selenium-supplemented control (6 mg/kg/day sodium selenite);
Group 3, swimming control; Group 4, selenium-supplemented swimming (6 mg/kg/day sodium selenite). Liver tissue samples collected
from the animals at the end of the study were fixed in 95% ethyl alcohol. From the tissue samples buried into paraffin, 5-μm
cross-sections were obtained using a microtome, put on a microscope slide, and stained with PAS. Stained preparations were
assessed using a Nikon Eclipse E400 light microscope. All images obtained with the light microscope were transferred to a
PC and evaluated using Clemex PE 3.5 image analysis software. The highest liver glycogen levels were found in groups 1 and
2 (p < 0.05). The levels in group 4 were lower than those in groups 1 and 2 but higher than the levels in group 3 (p < 0.05). The lowest liver glycogen levels were obtained in group 3 (p < 0.05). Results of the study indicate that liver glycogen levels that decrease in acute swimming exercise can be restored
by selenium administration. It can be argued that physiological doses of selenium administration can contribute to performance. 相似文献
9.
A 42-day experiment was conducted to compare the effects of various levels of sodium selenite (SS) and Se-enriched yeast (SY)
on chicken productivity, carcass traits, and breast Se concentration. Six hundred 1-day-old Cobb 500 broiler chicks were placed
on 1 of 6 experimental treatments. The treatments consisted of feeding a diet without Se supplementation (basal diet) or basal
diet with 0.6 mg/kg supplemented Se supplied by SS, SY, or a mix of the two (0.45 SS + 0.15 SY; 0.3 SS + 0.3 SY; 0.15 SS + 0.45
SY). Chicks in all Se-supplemented treatments had significantly higher final body weight and eviscerated weight than those
on the basal diet (P < 0,05) and no significant differences were observed among selenium source (P < 0.05). Also, chicks in all Se-supplemented treatments had significantly higher Se contents in breast tissue than the control
group (P < 0.05). Replacing SS by SY in the broiler diets resulted in increased concentrations of Se in the breast (P < 0.01). Strong correlations were found between breast Se concentrations and the level of SY supplementation of the broiler
diet (r = 0.992). The results from this experiment indicate that SY is a superior source of selenium for the production of selenized
meat, and can be used, without any detrimental effect on chicken performance, for adding nutritional value to broiler meat
and thus safely improving human selenium intake. 相似文献
10.
Suresh K. Gupta Vivekananthan Kalaiselvan Sushma Srivastava Rohit Saxena Shyam S. Agrawal 《Biological trace element research》2010,136(3):258-268
Cataract is the opacification in eye lens and leads to 50% of blindness worldwide. The present study was undertaken to evaluate
the anticataract potential of Trigonella foenum-graecum Linn seeds (fenugreek) in selenite-induced in vitro and in vivo cataract. In vitro enucleated rat lenses were maintained
in organ culture containing Dulbecco’s modified Eagles medium (DMEM) alone or in addition with 100 μM selenite and served
as the normal and control groups, respectively. For the test group, the medium was supplemented with selenite and T. foenum-graecum aqueous extract. The lenses were incubated for 24 h at 37°C. After incubation, the lenses were processed for the estimation
of reduced glutathione (GSH), lipid peroxidation product (malondialdehyde), and the antioxidant enzymes. In vivo selenite
cataract was induced in 9-day-old rats by subcutaneous injection of sodium selenite (25 μmol/kg body weight). Animals in the
test group were injected with different doses of aqueous extract of T. foenum-graecum 4 h before the selenite challenge. A fall in GSH and a rise in malondialdehyde levels were observed in control as compared
to normal lenses. T. foenum-graecum significantly (P < 0.01) restored glutathione and decreased malondialdehyde levels. A significant restoration in the activities of antioxidant
enzymes such as superoxide dismutase (P < 0.01), catalase, (P < 0.01), glutathione peroxidase (P < 0.01), and glutathione-S-transferase (P < 0.01) was observed in the T. foenum-graecum supplemented group as compared to control. In vivo, none of the eyes was found with nuclear cataract in treated group as
opposed to 72.5% in the control group. T. foenum-graecum protects against experimental cataract by virtue of its antioxidant properties. Further studies are warranted to explore
its role in human cataract. 相似文献
11.
Effect of Different Selenium Sources on Productive Performance,Serum and Milk Se Concentrations,and Antioxidant Status of Sows 总被引:2,自引:0,他引:2
Huijuan Hu Min Wang XiuAn Zhan Xing Li RuQian Zhao 《Biological trace element research》2011,142(3):471-480
The experiment was conducted to study the effects of different selenium (Se) sources on productive performance, serum and
milk Se concentrations, and antioxidant status of sows. A total of 12 sows (Landrace×Yorkshire) with same pregnancy were randomly
divided into two groups; each group was replicated six times. These two groups received the same basal gestation and lactation
diets containing 0.042 mg Se/kg, supplemented with 0.3 mg Se/kg sodium selenite or selenomethionine (i.e., seneno-dl-methylseleno), respectively. The feeding trial lasted for 60 days, with 32 and 28 days for gestation and lactation period,
respectively. Compared with sodium selenite, maternal selenomethionine intake significantly increased (P < 0.05) the weaning litter weight and average weight of piglet. The Se concentration in the serum, colostrum, and milk of
sows were significantly higher (p < 0.05) in the selenomethionine-treated group. The antioxidant status was greatly improved in sows of selenomethionine-treated
group and was illuminated by the increased total antioxidant capability (T-AOC; P < 0.05) and decreased malondialdehyde (MDA; P < 0.01) level in the serum of sows, increased T-AOC (P < 0.05), glutathione (GSH) peroxidase (P < 0.05), superoxide dismutase (P < 0.05) and GSH (P < 0.05), and MDA (P < 0.05) level in the colostrum and milk of sows. These results suggested that maternal selenomethionine intake improved Se
concentration and antioxidant status of sows, thus maintain maternal health and increase productive performance after Se was
transferred to its offspring. 相似文献
12.
Mohamed A. Haidara Mohamed D. Morsy Hesham A. Abdel-Razek Dimitri P. Mikhailidis Esma R. Isenovic 《Journal of physiology and biochemistry》2010,66(3):255-264
Septicemia leads to oxidative stress with overproduction of reactive-oxygen species (ROS) and consumption of endogenous antioxidant
enzymes. We tested a twofold hypothesis: (1) does oxidative stress (OxS) induced by sepsis acting alone or in concert with
augmented inflammatory processes contributes to sepsis-related vascular dysfunction, and, (2) whether ozone (O3) and l-canavanine (CAV) mitigate the negative impact of the aforementioned phenomena. We investigated the relative impact of treatment
with CAV and/or O3 on vascular OxS associated vascular functional changes in septicemic rats. For this study, 60 male Sprague–Dawley rats were
used and divided into six experimental groups (n = 10): control group (C), sham-operated (Sham), septicemic rats (S), S rats treated with CAV (100 mg/kg. i.p; S + CAV), S
rats treated with O3 (1.2 mg/kg, i.p.; S + O3) and S rats treated with both O3 and CAV (S + O3 + CAV). After 22 h, the mean arterial blood pressure (MAP), the aortic ring vascular reactivity to phenylephrine, abdominal
aortic blood flow (AABF), serum tumor necrosis factor-α (TNF-α) and plasma nitrite/nitrate (NOx) concentration were measured.
In addition, hepatic antioxidant enzyme activities sodium dismutase (SOD) and glutathione peroxidase (GSH-Px) were estimated.
Septicemia caused significant elevation of serum TNF-α (p < 0.001) and plasma NOx (p < 0.001) and significant (p < 0.001) reduction of AABF (p < 0.001), aortic vascular response to phenylephrine (p < 0.001), MAP (p < 0.001) and hepatic SOD and GSH-Px activity (p < 0.001) compared with the C group, while treatment with O3 and/or CAV induced significant amelioration of all those increases. Abnormalities were attenuated to a similar extent with
treatment with both O3 and CAV. These results suggested that concomitant administration of O3 and CAV alleviated the compromised vascular reactivity in septicemic conditions and prevent its progression into septic shock
compared with each alone. 相似文献
13.
14.
Effect of different selenium source (sodium selenite and selenium yeast) on broiler chickens 总被引:6,自引:0,他引:6
A feeding experiment was carried out to compare the effects of supplementing a poultry meal-based diet with selenium as sodium selenite or selenium yeast on broiler chickens. Three groups with three replicates of broiler chickens (mean weight 710 ± 5.3 g) were given a basal diet either unsupplemented (control) or supplemented with 0.2 mg Se kg−1 as sodium selenite (trial 1) or selenium yeast (trial 2) respectively, for 21 days. There was significant difference (P<0.05) in Feed Conversion Ratio (FCR) of trials 1 and 2 compared with the control. However, there were no significant differences (P>0.05) in FCR between trials 1 and 2. Final weight, survival rate and Daily Gain (DG) were not affected by the dietary Se source. Chickens fed the basal diet showed lower (P<0.05) selenium content in muscle, kidney, liver and pancreas compared to that fed selenium supplements (trials 1 and 2). Furthermore, trial 2 showed the highest value (P<0.05) among these treatments. However, there was no significant difference (P>0.05) in muscle selenium content of chickens between trials 1 and 2. Glutathione peroxidase (GSH-Px) activities in broiler chickens plasma and liver of all selenium treatment groups (trials 1 and 2) were significantly different (P<0.05) from that of the control. The GSH-Px activity in plasma was higher (P<0.05) in trial 2 compared with trial 1 and the control. However, there was no difference (P>0.05) in hepatic glutathione peroxidase between trials 1 and 2 although the average value of GSH-Px activity in trial 2 presented the trend of increase. 相似文献
15.
Mustafa Akil Umit Gurbuz Mursel Bicer Abdullah Sivrikaya Rasim Mogulkoc Abdulkerim Kasim Baltaci 《Biological trace element research》2011,142(3):651-659
The present study aims to evaluate the effect of selenium supplementation on lipid peroxidation and lactate levels in rats
subjected to acute swimming exercise. Thirty-two adult male rats of Sprague–Dawley type were divided into four groups. Group
1, control; group 2, selenium-supplemented; group 3, swimming control; group 4, selenium-supplemented swimming group. The
animals in groups 2 and 4 were supplemented with (i.p.) 6 mg/kg/day sodium selenite for 4 weeks. The blood samples taken from
the animals by decapitation method were analyzed in terms of erythrocyte-reduced glutathione (GSH), serum glutathione peroxidase
(GPx) and superoxide dismutase (SOD), and plasma malondialdehyde (MDA) and lactate using the colorimetric method, and serum
selenium values using an atomic emission device. In the study, the highest MDA and lactate values were found in group 3, while
the highest GSH, GPx and SOD values were obtained in group 4 (p < 0,001). Group 2 had the highest and group 3 had the lowest selenium levels (p < 0,001). Results of the study indicate that the increase in free radical production and lactate levels due to acute swimming
exercise in rats might be offset by selenium supplementation. Selenium supplementation may be important in that it supports
the antioxidant system in physical activity. 相似文献
16.
Souvik Roy Sudheer Kumar Dontamalla Anil Kumar Mondru Santanu Sannigrahi Prabhakar Reddy Veerareddy 《Biological trace element research》2011,139(1):55-71
To investigate whether sodium selenate treatment would impact on the onset of diabetic nephropathy, we examined blood glucose,
serum biochemical components, and interrelationship between oxidative stress, TGF-β1, and apoptosis in streptozotocin (STZ)
induced diabetic rats. Sixty male Wistar rats were divided into six groups. Group I (n = 10), normal control; Group II (n = 10), diabetic control; Group III (n = 10), sodium selenate (16 μmoles/kg) + diabetic; Group IV (n = 10), sodium selenate (32 μmoles/kg) + diabetic; Group V (n = 10), sodium selenate (16 μmoles/kg) control; and Group VI (n = 10), sodium selenate (32 μmoles/kg) control. Sodium selenate was administered via orogastric route for 10 weeks. In the
diabetic group, diabetes was induced by single intraperitoneal injection of STZ (50 mg/kg). The levels of blood glucose were
estimated and total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, creatinine, urea, and albumin
were detected in serum. Antioxidant status was examined by measuring the superoxide dismutase (SOD), catalase, glutathione,
and lipid peroxidation in kidney tissues. Histopathological studies were performed in the kidney tissue sections. The expression
of TGF-β1 was estimated by the immunohistochemical analysis in kidneys. Apoptotic study in kidney was performed using the
TdT-mediated dUTP nick end labeling technique. It was observed that blood glucose, serum, total cholesterol, HDL cholesterol,
triglycerides, creatinine, urea, and albumin were significantly higher in diabetic control groups. Diabetic + sodium selenate
(16 and 32 μmoles/kg) significantly reduced blood glucose, serum, total cholesterol, HDL cholesterol, triglycerides, creatinine,
urea, and albumin levels. Selenium-treated groups significantly increased antioxidant enzyme activities (SOD, catalase, and
glutathione) in kidneys of diabetic rats. All enzyme activities of selenium control groups did not differ compared with the
normal control. Sodium selenate reduces significantly lipid peroxidation in diabetic rats. Cellular architecture of the diabetic
rats was altered whereas sodium selenate administration rectifies the degenerative changes of the kidney. Profound immunopositivity
of TGF-β1 was observed in the glomerular and tubulointerstitial cells of diabetic rat kidney. Immunopositivity of TGF-β1 was
significantly reduced in both low and high dose of sodium-selenate-treated rats (P < 0.05, P < 0.01). High numbers of apoptotic cells were observed in diabetic rats whereas sodium selenate in both doses significantly
reduces the incidence of apoptosis (P < 0.05, P < 0.01). We conclude herein that sodium selenate has the potential to play a significant role in limiting the renal impairment
by altering the apoptosis and TGF-β1 in experimental diabetic rats. 相似文献
17.
Twenty male buffalo calves (15 months, 200.2 ± 9.75) were divided into four groups of five animals in each and fed diets without
(T1) or supplemented with 0.3 ppm selenium (Se) + 40 ppm zinc (Zn) (T2), 0.3 ppm Se + 40 ppm Zn + 10 ppm copper (Cu) (T3),
and 40 ppm Zn + 10 ppm Cu (T4) for 120 days, during which blood samples were collected on days 0, 40, 80, and 120. Concentrations
of glucose, total protein, albumin, globulin, urea, uric acid, and creatinine were similar in all the four groups. The level
of different serum enzymes viz. lactate dehydrogenase, alkaline phosphatase, glutamate pyruvate transaminase, and glutamate
oxaloacetate transaminase, and hormones viz. T3, T4, testosterone and insulin were similar (P > 0.05) among the four groups but the ratio of T4/T3 was reduced (P < 0.05) in the groups (T2 and T3) where selenium was supplemented at 120th day of supplementation. It was deduced that supplementation
of 0.3 ppm Se and/or 10.0 ppm of Cu with 40 ppm Zn had no effect on blood metabolic profile in buffalo calves, except the
ratio of T4 and T3 hormone which indicates that selenium plays an important role in converting T4 hormone to T3 which is more active form of thyroid hormone. 相似文献
18.
Wei XJ Wu J Ni YD Lu LZ Zhao RQ 《In vitro cellular & developmental biology. Animal》2011,47(10):735-741
Previous studies have shown that the in ovo injection of equol can markedly improve the water-holding capacity of muscles
of broilers chickens at 7 wk of age through promotion of the antioxidant status. We aimed to investigate directly the antioxidant
effects of equol on muscle cells in broilers. Muscle cells were separated from leg muscle of embryos on the 11th day of incubation
and treated with equol and H2O2, either alone or together. Cells were pretreated with medium containing 1, 10, or 100 μM equol for 1 h prior to the addition
of 1 mM H2O2 for a further 1 h. Photomicrographs of cells were obtained. Cell viability, malondialdehyde (MDA) content, and L-lactate
dehydrogenase (LDH) activity in the cell supernatant, as well as intracellular total superoxide dismutase (T-SOD) and glutathione
peroxidase (GSH-Px) activities were determined. Treatment with 1 mM H2O2 caused serious damage to cells, indicated by comets with no clear head region but a very apparent tail of DNA fragments.
Pretreatment with low (1 μM) but not high concentrations of equol (10 μM) inhibited cell damage, while 100 μM equol caused
more serious damage than H2O2 alone. Pretreatment with 1 μM equol had no effect on cell viability, while pretreatment with 10 and 100 μM equol significantly
decreased cell viability in a dose-dependent manner. Compared with H2O2 alone, pretreatment with low-dosage equol markedly decreased LDH activity and MDA production in the supernatant, significantly
increased intracellular T-SOD activity (P < 0.05) and tended to increase intracellular GSH-Px activity (0.05 < P < 0.1). Pretreatment with high-dosage equol (10 and 100 μM) significantly enhanced LDH activity, but had no effect on MDA
content, T-SOD or GSH-Px activity induced by H2O2, except for an obvious increase in GSH-Px activity caused by 10 μM equol. These results indicate that equol at low dosage
can prevent skeletal muscle cell damage induced by H2O2, while pretreatment with high-dosage equol shows a synergistic effect with H2O2 in inducing cell damage. 相似文献
19.
Woravimol Krittaphol Arlene McDowell Christine D. Thomson Momir Mikov J. Paul Fawcett 《Biological trace element research》2011,139(2):188-196
l-Selenomethionine (SeMet) and sodium selenite are widely used selenium nutritional supplements with potential benefit in preventing
cancer. However, supplementation is not without risks of toxicity if intake is too high. The aim of the present study was
to investigate SeMet and selenite metabolism in the gastrointestinal tract with particular focus on the formation of the volatile
selenium excretion products, dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). Adult male Wistar rats (n = 5) were euthanized, their intestinal tracts removed and the contents of jejunum, ileum, caecum and colon used to prepare
10% suspensions in saline. SeMet and selenite (0.5–0.6 mM) were then incubated with these suspensions at 37°C for 3 h. Caecum
and colon contents were the most metabolically active towards SeMet with 30% and 15% metabolized over 3 h. DMDSe was the only
volatile selenium metabolite detected accounting for 8.7 ± 1.3% of the selenium lost in caecum contents. Selenite was completely
metabolized by caecum contents and 73% by colon contents under the same conditions forming DMSe (5.7 ± 0.9% of the selenium
lost in caecum) and a precipitate of red amorphous elemental selenium. Based on previous literature and these results, we
conclude that the gut microbiota contributes to the excretion of excess selenium through the production of methylated selenium
compounds and elemental selenium. 相似文献
20.
Effects of Selenium with Vitamin E and Melatonin on Cadmium-Induced Oxidative Damage in Rat Liver and Kidneys 总被引:2,自引:0,他引:2
The present study was performed to determine the protective effects of melatonin alone and vitamin E with selenium combination
against cadmium-induced oxidative damage in rat liver. A total of 60 male rats were equally divided into five groups, one
of which acted as control receiving subcutaneous injections of physiological saline. The remaining four groups were treated
with subcutaneous injections of cadmium chloride at a dose of 1 mg/kg weight. The first study group received no treatment.
The second group was treated with a combination of 60 mg/kg vitamin E and 1 mg/kg sodium selenite. Group 3 was treated with
10 mg/kg melatonin, and the four group received a combination of vitamin E, sodium selenite, and melatonin at the doses mentioned
above. After 1 month, the animals were killed, and liver and kidneys were excised for histopathological inspection and determination
of tissue malondialdehyde and the activity of superoxide dismutase. The animals receiving no treatment showed significantly
higher malondialdehyde levels and reduced activity of superoxide dismutase (p < 0.05). Treatment with antioxidants resulted in a significant reduction in malondialdehyde when compared to nontreated animals
(p < 0.05) and increase in the enzyme activity that was almost the same as the controls. The pathological findings were also
in parallel with the results of the biochemical analysis. In conclusion, all the agents tested had protective effects against
cadmium-induced oxidative damage. 相似文献