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1.
Lemna paucicostata in Japan is classified into 4 types, N-1, N-2, K and S types. S type strains which are distributed in southern Japan grew
more rapidly than other strains at the temperatures of both 27 and 17C. With the extension of the culture period at 17 C to
which the plants had been transferred from 25C, the growth rate increased greatly in S type strains, while it decreased in
N-1 type strains which are distributed widely from Hokkaido to Kyushu, and particularly in those distributed in the northern
area. S type strains survived winter mostly in the form of normal fronds under natural conditions at Kyoto (minimum temperature
is lower than OC), while most of the fronds of N-1 type strains died after producing seed before winter. Thus S type strains
are considered to have adapted to the climate of southern Japan so as to survive winter in the form of fronds, while N-1 type
strains overwinter in the form of seed. N-2 type strains which are distributed in the northern part of the area adjacent to
the Japan Sea produced turion-like fronds during winter under natural conditions, while none of the other strains did. The
K type strain which was found only at the campus of Kyoto University overwintered only in the form of fronds which produce
no seed. 相似文献
2.
Flowering behavior of 22 strains ofLemna paucicostata collected in Japan by Yukawa and Takimoto (1976) was re-examined. The critical dark periods of the short-day strains (N-1
and N-2 types) were shorter than those determined by Yukawa and Takimoto except for that of one strain. Particularly in strains
391, 381 and 321, the differences were as large as 2.25, 1.75 and 1.5 hr, respectively. Such differences were found to be
due at least partly to the difference in night temperature; 25 C for the light and 23 C for the dark periods in the present
experiment, and 25 C throughout the light and dark periods in the previous experiment. The S type strains did not flower under
our experimental conditions (fluorescent light of 6,000 lux at 25 C) at any photoperiod tested, but flowered as a quantitative
long-day plant under natural daylight or high-intensity light (12,000 lux). Addition of sucrose or ammonium ion to the medium
suppressed the flowering of these strains under high-intensity light.
Addition of benzoic acid (1–5 μM) to 0.5 strength NH4
+-free Hutner's medium caused daylength-independent flowering in some N-1 type strains and in all N-2 type strains tested.
S type strains cultured under fluorescent light of 6,000 lux also flowered rapidly in response to benzoic acid. 相似文献
3.
Twenty-two strains ofLemna paucicostata collected from various districts in Japan are classified into 4 types having different morphological features and photoperiodic
behaviors. (I) The “N-1” type, widely distributed in north and middle Japan, consists of many short-day strains with similar
morphological characteristics. The strain of northern origin, however, has a shorter critical dark period than that of southern
origin, and most of the strains flower in response to a single short day though some from middle Japan require 2 short days.
(2) The “N-2” type, found in a limited area of northern Japan, is also a short-day type, but has very long critical dark periods
and requires 3–5 short-day cycles for flowering. (3) The “S” type, distributed in southern Japan, never flowered in any photoperiod
or culture conditions tested. (4) The “K” type, strain 351, collected at the experimental farm of Kyoto University, is a day-neutral
type and flowers independently of photoperiod.
Application of some SH inhibitors, tungstate or EDTA, or exposure to blue, far-red or low-intensity light caused daylength-independent
flowering in short-day strain 6746 (California origin), but none of these treatments caused flowering in any short-day strain
collected in Japan. 相似文献
4.
A methanogen, strain AK-1, was isolated from permanently cold marine sediments, 38- to 45-cm below the sediment surface at
Skan Bay, Alaska. The cells were highly irregular, nonmotile coccoids (diameter, 1 to 1.2 μm), occurring singly. Cells grew
by reducing CO2 with H2 or formate as electron donor. Growth on formate was much slower than that on H2. Acetate, methanol, ethanol, 1- or 2-propanol, 1- or 2-butanol and trimethylamine were not catabolized. The cells required
acetate, thiamine, riboflavin, a high concentration of vitamin B12, and peptones for growth; yeast extract stimulated growth but was not required. The cells grew fastest at 25 °C (range 5
°C to 25 °C), at a pH of 6.0 – 6.6 (growth range, pH 5.5 – 7.5), and at a salinity of 0.25 – 1.25 M Na+. Cells of this and other H2-using methanogens from saline environments metabolized H2 to a very low threshold pressure (less than 1 Pa) that was dependent on the methane partial pressure. We propose that the
threshold pressure may be limited by the energetics of catabolism. The sequence of the 16S rDNA gene of strain AK-1 was most
similar (98%) to the sequences of Methanogenium cariaci JR-1 and Methanogenium frigidum Ace-2. DNA–DNA hybridization between strain AK-1 and these two strains showed only 34.9% similarity to strain JR-1 and 56.5%
similarity to strain Ace-2. These analyses indicated strain AK-1 should be classified as a new species within the genus Methanogenium. Phenotypic differences between strain AK-1 and these strains (including growth temperature, salinity range, pH range, and
nutrient requirements) support this. Therefore, a new species, Methanogenium marinum, is proposed with strain AK-1 as type strain.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
5.
Paavo Ahvenniemi Matthias Wolf Mari J. Lehtonen Paula Wilson Malgorzata German-Kinnari Jari P. T. Valkonen 《Journal of molecular evolution》2009,69(2):150-163
The rRNA cistron (18S–ITS1–5.8S–ITS2–28S) is used widely for phylogenetic analyses. Recent studies show that compensatory
base changes (CBC) in the secondary structure of ITS2 correlate with genetic incompatibility between organisms. Rhizoctonia solani consists of genetically incompatible strain groups (anastomosis groups, AG) distinguished by lack of anastomosis between
hyphae of strains. Phylogenetic analysis of internal transcribed spacer (ITS) sequences shows a strong correlation with AG
determination. In this study, ITS sequences were reannotated according to the flanking 5.8S and 28S regions which interact
during ribogenesis. One or two CBCs were detected between the ITS2 secondary structure of AG-3 potato strains as compared
to AG-3 tobacco strains, and between these two strains and all other AGs. When a binucleate Rhizoctonia species related to Ceratobasidiaceae was compared to the AGs of R. solani, which were multinucleate (3–21 nuclei per cell), 1–3 CBCs were detected. The CBCs in potato strains of AG-3 distinguish
them from AG-3 tobacco strains and other AGs yielding further evidence that the potato strains of AG-3 originally described
as R. solani are a species distinct from other AGs. The ITS1–5.8S–ITS2 sequences were analyzed by direct sequencing of PCR products from
497 strains of AG-3 isolated from potato. The same 10 and 4 positions in ITS1 and ITS2, respectively, contained variability
in 425 strains (86%). Nine different unambiguous ITS sequences (haplotypes) could be detected in a single strain by sequencing
cloned PCR products indicating that concerted evolution had not homogenized the rRNA cistrons in many AG-3 strains. Importantly,
the sequence variability did not affect the secondary structure of ITS2 and CBCs in AG-3.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
6.
Da-Chun Zhuang Yi-Guang Chen Yu-Qin Zhang Shu-Kun Tang Xiao-Lei Wu Zhou-Cai Tan Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(3):295-301
A novel Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped, aerobic bacterium, designated strain JSM
078120T, was isolated from sea water collected from a tidal flat of Naozhou Island, South China Sea. Growth occurred with 1–15% (w/v)
total salts (optimum, 2–4%), at pH 6.0–10.0 (optimum, pH 7.5) and at 4–35°C (optimum, 25–30°C). The major cellular fatty acids
were C18:1
ω9c, C16:0, C12:0 3-OH and C16:1
ω7c. The predominant respiratory quinone was ubiquinone Q-9, and the genomic DNA G + C content was 60.6 mol%. Phylogenetic analysis
based on 16S rRNA gene sequences indicated that strain JSM 078120T should be assigned to the genus Marinobacter, being related most closely to the type strains of Marinobacter segnicrescens (sequence similarity 98.2%), Marinobacter bryozoorum (97.9%) and Marinobacter gudaonensis (97.6%). The sequence similarities between the novel isolate and the type strains of other recognized Marinobacter species ranged from 96.7 (with Marinobacter salsuginis) to 93.3% (with Marinobacter litoralis). The levels of DNA–DNA relatedness between strain JSM 078120T and the type strains of M. segnicrescens, M. bryozoorum and M. gudaonensis were 25.3, 20.6 and 18.8%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics
and chemotaxonomic data supported the view that strain JSM 078120T represents a novel species of the genus Marinobacter, for which the name Marinobacter zhanjiangensis sp. nov. is proposed. The type strain is JSM 078120T (= CCTCC AB 208029T = DSM 21077T = KCTC 22280T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078120T is FJ425903. 相似文献
7.
Two morphologically distinct strains, 63–76 and 63H1, were isolated from a protoplast and a hyphal tip of the parentalSclerotium rolfsii strain S-63, respectively. Strains 63–76 and 63H1 showed reduced mycelial growth and lacked clamp connections on hyphae.
The two strains also differed from each other and from their parent in RAPD patterns generated by several primers, suggesting
that 63–76 and 63H1 were homokaryons isolated from the hetereokaryon S-63. Whereas the parent S-63 belonged to ITS-RFLP group
1, RFLP patterns of internal transcribed spacer (ITS) regions of rDNA of 63–76 and 63H1 were similar to those of ITS-RFLP
groups 5 and 3, respectively. The sequence similarity of ITS regions were more than 99% between 63–76 and group 5 strains,
100% between 63H1 and the group 3 strain, and 96.3% between 63–76 and 63H1. Direct sequencing failed in the parental strain
S-63. S-63 was considered to contain ITS types of groups 5 and 3. 相似文献
8.
Yun Jae Kim Jhung-Ahn Yang Jae Kyu Lim Mi-Jeong Park Sung-Hyun Yang Hyun Sook Lee Sung Gyun Kang Jung-Hyun Lee Kae Kyoung Kwon 《Journal of microbiology (Seoul, Korea)》2020,58(4):252-259
An anaerobic, rod-shaped, mesophilic, chemolithoautotrophic, sulfate-reducing bacterial strain IOR2T was isolated from a newly found deep-sea hydrothermal vent (OVF, Onnuri Vent Field) area in the central Indian Ocean ridge (11°24′88″ S 66°25′42″ E, 2021 m water depth). The 16S rRNA gene sequence analysis revealed that the strain IOR2T was most closely related to Desulfovibrio senegalensis BLaC1T (96.7%). However, it showed low similarity with the members of the family Desulfovibrionaceae, such as Desulfovibrio tunisiensis RB22T (94.0%), D. brasiliensis LVform1T (93.9%), D. halophilus DSM 5663T (93.7%), and Pseudodesulfovibrio aespoeensis Aspo-2T (93.2%). The strain IOR2T could grow at 23–42°C (optimum 37°C), pH 5.0–8.0 (optimum pH 7.0) and with 0.5–6.5% (optimum 3.0%) NaCl. The strain could use lactate, pyruvate, H2, and glycerol as electron donors and sulfate, thiosulfate, and sulfite as electron acceptors. The major fatty acids of the strain IOR2T were iso-C15:0, iso-C17:0, ante-iso-C15:0, and summed feature 9 (C16:0 methyl/iso-C17:1ω9c). Both the strains IOR2T and BLaC1T could grow with CO2 and H2 as the sole sources of carbon and energy, respectively. Genomic evidence for the Wood-Ljungdahl pathway in both the strains reflects chemolithoautotrophic growth. The DNA G + C content of the strain IOR2T and BLaC1T was 58.1–60.5 mol%. Based on the results of the phylogenetic and physiologic studies, Paradesulfovibrio onnuriensis gen. nov., sp. nov. with the type strain IOR2T (= KCTC 15845T = MCCC 1K04559T) was proposed to be a member of the family Desulfovibrionaceae. We have also proposed the reclassification of D. senegalensis as Paradesulfovibrio senegalensis comb. nov. 相似文献
9.
Zhao W Weber C Zhang CL Romanek CS King GM Mills G Sokolova T Wiegel J 《Extremophiles : life under extreme conditions》2006,10(4):337-345
A novel thermophilic, alkali-tolerant, and CO-tolerant strain JW/WZ-YB58T was isolated from green mat samples obtained from the Zarvarzin II hot spring in the Uzon Caldera, Kamchatka (Far East Russia). Cells were Gram-type and Gram stain-positive, strictly aerobic, 0.7–0.8 μm in width and 5.5–12 μm in length and produced terminal spherical spores of 1.2–1.6 μm in diameter with the mother cell swelling around 2 μm in diameter (drumstick-type morphology). Cells grew optimally at pH25°C 8.2–8.4 and temperature 50–52°C and tolerated maximally 6% (w/v) NaCl. They were strict heterotrophs and could not use either CO or CO2 (both with or without H2) as sole carbon source, but tolerated up to 90% (v/v) CO in the headspace. The isolate grew on various complex substrates such as yeast extract, on carbohydrates, and organic acids, which included starch, d-galactose, d-mannose, glutamate, fumarate and acetate. Catalase reaction was negative. The membrane polar lipids were dominated by branched saturated fatty acids, which included iso-15:0 (24.5%), anteiso-15:0 (18.3%), iso-16:0 (9.9%), iso-17:0 (17.5%) and anteiso-17:0 (9.7%) as major constituents. The DNA G+C content of the strain is 45 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain JW/WZ-YB58T is distantly (<93% similarity) related to members of Bacillaceae. On the basis of 16S rRNA gene sequence, physiological and phenotypic characteristics, the isolate JW/WZ-YB58T (ATCC BAA-1258; DSM 17740) is proposed to be the type strain for the type species of the new taxa within the family Bacillaceae, Thermalkalibacillus uzoniensis gen. nov. sp. nov. The Genbank accession number for the 16S rRNA gene sequence is DQ221694.The Genbank accession number for the 16S rRNA gene sequence of strain JW/WZ-YB58T is DQ221694. 相似文献
10.
Thirty-one bradyrhizobial and rhizobial strains infecting pigeon pea were screened for siderophore production using Chrome
Azurol S (CAS) agar plate as well as a CAS assay solution. Of a total of 31 strains only 23 showed siderophore production.
Of the 23 siderophore-positive strains, 21 strains showed the production of hydroxamate while 6 strains showed the presence
of catechol type of siderophore. A large variation in the quantity of hydroxamate and catechol produced by different rhizobial
strains was observed (1.03–3.73 μg hydroxamate N per mg protein; 0.19–3.43 μmol/L of catechol per mg protein). Maximum nodule
biomass was produced by strain PP-11 (CC-1020); strain G-14 formed minimum nodule biomass. Nitrogen contents of low, moderate
and high siderophore-producing strains were 11.4, 15.4, 20.9 mg per plant, respectively, iron contents were 1445, 1768 and
2003 ppm, respectively. Siderophore production was related to N2-fixing efficiency. 相似文献
11.
Yi-Guang Chen Zhu-Xiang Liu De-Jiao Peng Yu-Qin Zhang Yong-Xia Wang Shu-Kun Tang Wen-Jun Li Xiao-Long Cui Yan-Qi Liu 《Antonie van Leeuwenhoek》2009,96(3):323-329
A Gram-positive, moderately halophilic, endospore-forming, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium,
designated strain JSM 089168T, was isolated from saline soil collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow
with 2–25% (w/v) total salts (optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 8.0) and 10–45°C (optimum, 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The strain contained MK-7 as the predominant menaquinone,
and diphosphatidylglycerol and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0, and the DNA G + C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that
strain JSM 089168T should be assigned to the genus Virgibacillus, being related most closely to the type strains of Virgibacillus carmonensis (sequence similarity 97.6%), Virgibacillus necropolis (97.3%) and Virgibacillus halodenitrificans (97.1%). Levels of DNA–DNA relatedness between strain JSM 089168T and the type strains of V. carmonensis, V. necropolis and V. halodenitrificans were 20.4, 14.3 and 12.0%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics
and chemotaxonomic data supported the view that strain JSM 089168T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus litoralis sp. nov. is proposed. The type strain is JSM 089168T (=DSM 21085T =KCTC 13228T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 089168T is FJ425909. 相似文献
12.
Huang HY Chen YG Wang YX Liu JH Tang SK Peng Q Wen ML Yu H Cui XL 《Extremophiles : life under extreme conditions》2008,12(6):829-835
A novel Gram-negative, slightly halophilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-C248T, was isolated from a sediment sample collected from a salt-lake in the Qaidam Basin in Qinghai, north-west China. Cells were
non-sporulating short rods, occurring singly or as doublets, motile with peritrichous flagella. Growth occurred with 1–15%
(w/v) NaCl [optimum 2–4% (w/v) NaCl], at pH 6.0–10.0 (optimum pH 7.5) and at 4–35°C (optimum 25–30°C). The major cellular
fatty acids were C18:1
ω7c, C12:0 3-OH, cyclo C19:0
ω8c, C16:0 and C16:1. The predominant respiratory quinone was Q-9 and the genomic DNA G + C content was 58.6 mol%. Phylogenetic analysis based
on 16S rRNA gene sequences indicated that strain YIM-C248T should be assigned to the genus Halomonas. The sequence similarities between the isolate and the type strains of members of the genus Halomonas were in the range of 92.5–97.5%. The combination of phylogenetic analysis, DNA–DNA hybridization data, phenotypic characteristics
and chemotaxonomic differences supported the view that strain YIM-C248T represents a new species of the genus Halomonas, for which the name Halomonas sediminis sp. nov. is proposed, with YIM-C248T (=CCTCC AA 207031 = KCTC 22167) as the type strain.
The GenBank/EMBL/DBBJ accession number for the 16S rRNA gene sequence of strain YIM-C248T is EU135707. 相似文献
13.
Yi-Guang Chen Zhi-Xiong Liu Yu-Qin Zhang You-Xiang Zhang Shu-Kun Tang Entomack Borrathybay Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(1):99-107
A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM
071068T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from the Naozhou Island on the Leizhou Bay in the South China Sea. Cells were motile by means of peritrichous
flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071068T was able to grow with 1–20% (w/v) total salts (optimum, 6–9%), at pH values of 6.0–10.0 (optimum, pH 7.5) and a temperature
range of 10–35°C (optimum, 25°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7 and the major cellular
fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C15:0. The genomic DNA G + C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed
that strain JSM 071068T belonged to the genus Halobacillus. The 16S rRNA gene sequence similarities between strain JSM 071068T and the type strains of the recognized Halobacillus species ranged from 97.9% (with Halobacillus alkaliphilus) to 95.3% (with Halobacillus kuroshimensis). The levels of DNA–DNA relatedness between the new isolate and the type strains of H. alkaliphilus, Halobacillus campisalis, Halobacillus halophilus and Halobacillus seohaensis were 25.6, 22.1, 10.8 and 13.2%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic
characteristics and chemotaxonomic data supported the view that strain JSM 071068T represents a new species of the genus Halobacillus, for which the name Halobacillus naozhouensis sp. nov. is proposed, with JSM 071068T (=DSM 21183T =KCTC 13234T) as the type strain.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 071068T is EU925615. 相似文献
14.
Pankaj Krishna Amita Arora M. Sudhakara Reddy 《World journal of microbiology & biotechnology》2008,24(12):3079-3085
We have isolated and characterized a xylanolytic actinomycete strain (RM1) from the extremely alkaline bauxite residue obtained
from National Aluminum Company Ltd., Damanjodi, India. The phenotypic features and complete sequence of 16S rRNA revealed
that this strain belong the genus Kocuria and showed 98% sequence similarity with Kocuria
aegyptia. The RM1 strain was able to grow at pH 10.5 in buffered and unbuffered media and utilize 40 different carbon substrates.
The RM1 strain under optimal conditions produced extracellular xylanase at 311 U/ml. The xylanase produced by RM1 showed a
wide range of temperature (30–85°C) and pH (4.5–9) tolerance by retaining 90% of its activity. This is the first report of
isolation of actinomycetes, Kocuria sp., which produces high amount of xylanase, from bauxite residue and offers a new source of xylanase-producing strains. 相似文献
15.
Simultaneous nitrification and denitrification by diverse <Emphasis Type="Italic">Diaphorobacter</Emphasis> sp. 总被引:2,自引:0,他引:2
Eight bacterial isolates closely related to Diaphorobacter sp. were isolated from activated biomass surviving on wastewater laden with dyes and nitro-substituted chemicals and were
identified by 16S rDNA sequence analysis. The isolates showed sequence similarity of 99–100% to other Diaphorobacter strains such as ZY 2006b, F2, NA5, PCA039, D. nitroreducens KSP4, and KSP3 and 98–99% sequence homology to D. nitroreducens NA10B (type strain JCM 11421). Neighbor-joining tree revealed that all the eight strains formed tight cluster together and
also showed close clustering with other Diaphorobacter strains. Isolates demonstrated the ability to perform simultaneous nitrification and denitrification under aerobic conditions.
Strains HPC 805, 815, 821, and 856 gave highest chemical oxygen demand removal (85–93%) and ammonia removal (92–96%), which
correlated well with higher growth rates of the cultures. Simultaneously, complete removal of nitrate supplied in the medium
in presence of ammonium and acetate (electron donor) was observed in addition to aerobic nitrite release from ammonium. Thus,
the above strains showed ability to perform partial nitrification followed by further aerobic removal of common intermediate
nitrite, which indicated their potential application in treatment systems for treatment of high-nitrogen-containing wastewaters. 相似文献
16.
Alma E. Cruz-Guerrero José Luis Olvera Mariano García-Garibay Lorena Gómez-Ruiz 《World journal of microbiology & biotechnology》2006,22(2):115-117
Summary Two strains of Kluyveromyces marxianus (A1 and A2) isolated from ‘aguamiel’ (agave sap) and one strain of K. lactis var. lactis (P7) isolated from ‘pulque’ (its fermented product), were studied to make a survey of inulinase production. The strains of K. marxianus A1 and A2 were the best producers of inulinase, giving up to 2.5 times more enzyme than the control hyperproducing strain K. marxianus CDBB-L-278, and showed lower catabolic repression than this. One strain isolated from pulque was identified as K. lactis var. lactis and was also an excellent inulinase producer, being the first strain of this species reported as such. These strains were very good inulinase producers and they had low susceptibility to catabolic repression probably because the source from which they were isolated was rich in sucrose and oligofructans. They can be used in the transformation of inulin to produce fructose and/or oligofructans. 相似文献
17.
Yi-Guang Chen Shu-Kun Tang Yu-Qin Zhang Zhao-Yang Li Lang-Bo Yi Yong-Xia Wang Wen-Jun Li Xiao-Long Cui 《Antonie van Leeuwenhoek》2009,96(1):63-70
A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium,
designated strain JSM 078085T, was isolated from sea water collected from the South China Sea. Strain JSM 078085T exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0–12% (w/v)
NaCl and at pH 6.0–9.5 and 4–35°C; optimum growth was observed at pH 7.0 and 25–30°C in the absence of NaCl. The peptidoglycan
type was A4α (l-Lys–l-Ala–l-Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085T contained menaquinone MK-9(H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major
polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0 and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that
strain JSM 078085T should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA–DNA
relatedness between strain JSM 078085T and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data
supported the view that strain JSM 078085T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085T (=DSM 21081T=KCTC 19430T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078085T is EU583729. 相似文献
18.
Annukka E. Mäkinen Anna H. Kaksonen Jaakko A. Puhakka 《Extremophiles : life under extreme conditions》2009,13(3):505-510
A thermophilic, rod-shaped, motile, Gram-positive, spore-forming bacterium strain 70BT was isolated from a geothermally active underground mine in Japan. The temperature and pH range for growth was 50–81°C (optimum
71°C) and 6.2–9.8 (optimum pH 7–7.5), respectively. Growth occurred in the presence 0–2% NaCl (optimum 1% NaCl). Strain 70BT could utilize glucose, fructose, mannose, mannitol, pyruvate, cellobiose and tryptone as substrates. Thiosulfate was used
as electron acceptor. Major whole-cell fatty acids were iso-C15:0, C16:0 DMA (dimethyl acetal), C16:0 and anteiso-C15:0. The G+C mol% of the DNA was 44.2%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the closest relatives
of strain 70BT were Thermosediminibacter oceani DSM 16646T (94% similarity) and Thermosediminibacter litoriperuensis DSM 16647 (93% similarity). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain 70BT represents a novel species in a new genus, for which the name Thermovorax subterraneus gen. nov., sp. nov. is proposed. The type strain of Thermovorax subterraneus is 70BT (=DSM 21563 = JCM 15541). 相似文献
19.
Adhyayan Sharan Shikha Nandan Singh Darmwal Rajeeva Gaur 《World journal of microbiology & biotechnology》2008,24(6):753-759
A total of 198 bacterial strains were isolated from various niches of saline–alkali soils, out of which 85 strains were able
to solubilize phosphate on plates at 30 °C. The strain RMLU-26, identified as Xanthomonas campestris, was the most efficient with its ability to solubilize P, subjected to N-methyl-N′-nitro-N-nitrosoguanidine (NTG) for development of mutants. The P solubilizing ability of X. campestris is reported for the first time. The wild type and mutant strains of X. campestris revealed a differential response to various stress factors (high pH, temperature, and salt concentration). The mutant strain
revealed maximum P solubilization (67.1%) at 30 °C and pH 8.0 while the wild type strain showed maximum solubilization (41.9%)
at 35 °C and pH 7.0. Percent P2O5 solubilization by both strains revealed a steep decline in tricalcium phosphate solubilization with an increase in NaCl concentration
from 0.5 to 10% along with a concomitant drop in pH of the medium from 8.0 to 4.5 in wild type and 4.0 in mutant strain. However,
a 1.5- to 2-fold increase in ‘P’ solubilization was observed in the mutant strain when compared to the wild type strain in
the presence of NaCl. The overall improved tolerance of the strains to alkalinity and salinity could be due to accumulation
and/or secretion of specific solute (xanthan). 相似文献
20.
Kazuhiro Kobayashi Yutaka Nakahori Kunihiko Mizuno Masashi Miyake Toshiyuki Kumagai Akira Honma Ikuya Nonaka Yusuke Nakamura Katsushi Tokunaga T. Toda 《Human genetics》1998,103(3):323-327
Fukuyama-type congenital muscular dystrophy (FCMD) is an autosomal recessive, severe muscular dystrophy associated with brain
anomalies. After our initial mapping of the FCMD locus to 9q31–33, we performed linkage disequilibrium analysis, which led
us to suspect that the FCMD gene lay within a region of less than 100 kb containing D9S2107. In the present study, we developed
two new microsatellites (D9S2170 and D9S2171) in close vicinity to D9S2107 and examined haplotypes of FCMD chromosomes by
using four markers (cen-D9S2105-D9S2170-D9S2171-D9S2107-tel). As 82% of the FCMD chromosomes that we examined shared the founder
haplotype (138–192–147–183) and 94% of the FCMD patients in our panel carried founder haplotypes on one or both chromosomes,
the data supported the hypothesis of a single founder of this disease in the Japanese population. Eight haplotypes different
from the founder’s were observed in FCMD chromosomes, indicating that eight different FCMD mutations in addition to the founder’s
have occurred in Japan. Moreover, we have detected several historical recombinations that have disrupted the founder haplotype
at D9S2105 or D9S2170 and conclude that the FCMD gene is probably located just centromeric to D9S2170.
Received: 16 May 1998 / Accepted: 10 June 1998 相似文献