共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
Fernando Rodríguez-Castañeda Nicolas Coudevylle Stefan Becker Nils Brose Teresa Carlomagno Christian Griesinger 《Biomolecular NMR assignments》2010,4(1):45-48
Ca2+-Calmodulin binding to the variable N-terminal region of the diacylglycerol/phorbol ester-binding UNC13/Munc13 family of proteins modulates the short-term synaptic plasticity characteristics in neurons. Here, we report the sequential backbone and side chain resonance assignment of the Ca2+-Calmodulin/Munc13-1458–492 peptide complex at pH 6.8 and 35°C (BMRB No. 15470). 相似文献
3.
4.
5.
6.
Yunchen Bi Hongwei Li Shoujin Fan Bin Xia Changwen Jin 《Biomolecular NMR assignments》2009,3(1):149-151
The mRNA degradation is an important regulatory mechanism which controls gene expression by limiting the number of translation
times. Previous studies demonstrated that this process is essential for organisms. Escherichia coli RNA pyrophosphohydrolase (RppH) is an enzyme that triggers mRNA degradation by removing the 5′ pyrophosphate, which is a
rate-determining step. In order to understand the molecular mechanism of the biological function, the structural information
of RppH is required. Herein, we report the resonance assignments of 1H, 15N, 13C atoms of the E. coli RppH. 相似文献
7.
8.
9.
Sagaya Theresa Leena Philominathan Osamu Matsushita John Brad Jordan Joshua Sakon 《Biomolecular NMR assignments》2008,2(2):127-129
1H, 15N, and 13C NMR assignments for 116 amino acids (Gly893-Lys1008) of a bacterial collagen-binding domain (CBD) derived from Clostridium histolyticum class I collagenase were accomplished. Clostridial collagenases hydrolyze insoluble collagen. One to three copies of collagen-binding
domains (CBDs) are present at their C-termini, each of which is the minimal segment required for the binding to the insoluble
substrate. CBD has been shown to be able to anchor fused growth factors for up to 10 days in vivo. Structural analysis of
the small domain with the unique function provides insights into designing a novel drug delivery vehicle by the rational drug
design. 相似文献
10.
Fusco G De Simone A Hsu ST Bemporad F Vendruscolo M Chiti F Dobson CM 《Biomolecular NMR assignments》2012,6(1):27-29
Human muscle acylphosphatase (mAcP) is an enzyme with a ferrodoxin-like topology whose primary role is to hydrolyze the carboxyl-phosphate
bonds of acylphosphates. The protein has been widely used as a model system for elucidating the molecular determinants of
protein folding and misfolding. We present here the full NMR assignments of the backbone and side chains resonances of mAcP
complexed with phosphate, thus providing an important resource for future solution-state NMR spectroscopic studies of the
structure and dynamics of this protein in the contexts of protein folding and misfolding. 相似文献
11.
Yi-Jan Lin Andreas Schmidt Noelia Inés Burgardt Alexandra Thiele Matthias Weiwad Christian Lücke 《Biomolecular NMR assignments》2013,7(2):325-329
A 25-residue elongation at the N-terminus endows parvulin 17 (Par17) with altered functional properties compared to parvulin 14 (Par14), such as an enhanced influence on microtubule assembly. Therefore the three-dimensional structure of this N-terminal elongation is of particular interest. Here, we report the nearly complete 1H, 13C and 15N chemical shift assignments of Par17. Subsequent chemical shift index analysis indicated that Par17 features a parvulin-type PPIase domain at the C-terminus, analogous to Par14, and an unstructured N-terminus encompassing the first 60 residues. Hence the N-terminus of Par17 apparently adopts a functionally-relevant structure only in presence of the respective interaction partner(s). 相似文献
12.
Laura K. Bailey Louise J. Campbell Katrina A. Evetts Keily Littlefield Eeson Rajendra Daniel Nietlispach Darerca Owen Helen R. Mott 《Biomolecular NMR assignments》2009,3(1):33-36
We report 1H, 13C and 15N resonance assignments for Binder of Arl Two (BART), an effector of the small G protein Arl2. The BMRB accession code is
15914. 相似文献
13.
14.
Lipocalin2 plays an important role in the innate immune system. In this article we report the backbone and side-chain resonance
assignments of rat lipocalin2 (rLcn2). These assignments provide a basis for determining the structure and dynamics of rLcn2.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
16.
Soraya Serrano Mariona Callís Maria Vilanova Antoni Benito Douglas V. Laurents Marc Ribó Marta Bruix 《Biomolecular NMR assignments》2013,7(1):13-15
Onconase® FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase® sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase’s® active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure–function relationships, we report here the nearly complete NMR 1H, 13C and 15N resonance chemical shift assignments at pH 5.2 and 35°C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase® is conserved in the FL-G zymogen. 相似文献
17.
Ian B. Robertson Isabelle Osuch David A. Yadin Penny A. Handford Sacha A. Jensen Christina Redfield 《Biomolecular NMR assignments》2014,8(1):189-194
Fibrillins are large extracellular glycoproteins that form the principal component of microfibrils. These perform a vital structural function in the extracellular matrix of many tissues. Fibrillins have also been implicated in mediating a number of protein–protein interactions, some of which may be significant in regulating growth factors such as transforming growth factor β. Here we present the backbone and side-chain 1H, 13C and 15N assignments for a 19 kDa protein fragment derived from the N-terminus of human fibrillin-1, encompassing four domains in total. These domains include the second and third epidermal growth factor-like (EGF) domains, the first hybrid domain (hyb1), and the first calcium-binding EGF domain of fibrillin-1. This region of fibrillin-1 is of particular interest as the hyb1 domain has been suggested to play a role in microfibril assembly, as well as several other protein–protein interactions. 相似文献
18.
To determine the three-dimensional solution structure of the calcium bound S100P protein, the backbone and side chain resonance assignments of the S100P protein have been reported based on triple-resonance experiments using uniformly [13C, 15N]-labeled calcium bound protein. 相似文献
19.
In this article, we report the resonance assignment of CTD-interacting domain (CID) of pre-mRNA down-regulation (Nrd)1 bound to Ser5-phosphorylated CTD (pSer5) of RNA Polymerase II. The presented assignment of backbone and side-chain resonances of the Nrd1 CID proton, carbon and nitrogen nuclei will allow studies of the structure and interaction of CID with carboxy-terminal domain (CTD) of the RNA polymerase II. 相似文献
20.
The carboxy-terminal pleckstrin homology (PH) domain recruits GRP1 to the plasma membrane through the specific binding to
phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3]. Here, we describe backbone and side chain assignments of the GRP1 PH domain determined by triple resonance experiments.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献