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G. M. A. Cançado F. T. S. Nogueira S. R. Camargo R. D. Drummond R. A. Jorge M. Menossi 《Biologia Plantarum》2008,52(3):475-485
To investigate the molecular mechanisms of Al toxicity, cross-species cDNA array approach was employed to identify expressed
sequence tags (ESTs) regulated by Al stress in root tips of Al-tolerant maize (Zea mays) genotype Cat100-6 and Al-sensitive genotype S1587-17. Due to the high degree of conservation observed between sugarcane
and maize, we have analyzed the expression profiling of maize genes using 2 304 sugarcane (ESTs) obtained from different libraries.
We have identified 85 ESTs in Al stressed maize root tips with significantly altered expression. Among the up-regulated ESTs,
we have found genes encoding previously identified proteins induced by Al stress, such as phenyl ammonia-lyase, chitinase,
Bowman-Birk proteinase inhibitor, and wali7. In addition, several novel genes up-and downregulated by Al stress were identified
in both genotypes. 相似文献
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F Aberger A P Costa-Pereira J F Schlaak T M Williams R F O'Shaughnessy G Hollaus I M Kerr A M Frischauf 《Genomics》2001,77(1-2):50-57
The combination of high and low density cDNA filter array technology potentially permits both the identification of subsets of induced genes and convenient and rapid multisample expression profiling of such subsets under a variety of conditions. The JAK/STAT1 pathway for IFN-gamma signaling in human cells has been well characterized, but the extent and importance of additional pathways remain to be established. Here, using high-density filter arrays of the RZPD UniGene set, we identified 18 novel IFN-gamma-inducible genes. Expression profiling was carried out using low-density arrays representing both novel and known IFN-gamma-inducible genes. Initial experiments failed to detect evidence for any novel non-JAK-dependent pathways in cells expressing a kinase-dead JAK2. The data, however, validated the potential of the combined methods in establishing rapid and convenient expression profiling of several hundred genes in response to any ligand of choice. 相似文献
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Large-scale analysis of gene expression profiles during early stages of root nodule formation in a model legume, Lotus japonicus. 总被引:2,自引:0,他引:2
Hiroshi Kouchi Kenshiro Shimomura Shingo Hata Atsuko Hirota Guo-Jiang Wu Hirotaka Kumagai Shigeyuki Tajima Norio Suganuma Akihiro Suzuki Toshio Aoki Makoto Hayashi Tadashi Yokoyama Takuji Ohyama Erika Asamizu Chikara Kuwata Daisuke Shibata Satoshi Tabata 《DNA research》2004,11(4):263-274
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Seven Lotus japonicus genes required for transcriptional reprogramming of the root during fungal and bacterial symbiosis 总被引:7,自引:0,他引:7
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Kistner C Winzer T Pitzschke A Mulder L Sato S Kaneko T Tabata S Sandal N Stougaard J Webb KJ Szczyglowski K Parniske M 《The Plant cell》2005,17(8):2217-2229
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Root nodule formation is controlled by plant hormones such as auxin. Auxin-repressed protein (ARP) genes have been identified in various plant species but their functions are not clear. We have isolated a full-length cDNA clone (EuNOD-ARP1) showing high sequence homology to previously identified ARP genes from root nodules of Elaeagnus umbellata. Genomic Southern hybridization showed that there are at least four ARP-related genes in the genome of E. umbellata. The cDNA clone encodes a polypeptide of 120 amino acid residues with no signal peptide or organelle-targeting signals, indicating that it is a cytosolic protein. Its cytosolic location was confirmed using Arabidopsis protoplasts expressing a EuNOD-ARP1:smGFP fusion protein. Northern hybridization showed that EuNOD-ARP1 expression was higher in root nodules than in leaves or uninoculated roots. Unlike the ARP genes of strawberry and black locust, which are negatively regulated by exogenous auxin, EuNOD-ARP1 expression is induced by auxin in leaf tissue of E. umbellata. In situ hybridization revealed that EuNOD-ARP1 is mainly expressed in the fixation zone of root nodules. 相似文献
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