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The metabolism of ribonucleic acid in normal and bacteriophage infected Escherichia coli 总被引:8,自引:0,他引:8
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MANSON LA 《Journal of bacteriology》1953,66(6):703-711
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Methylation of RNA in bacteriophage T4 infected Escherichia coli 总被引:4,自引:0,他引:4
J A Boezi R L Armstrong M De Backer 《Biochemical and biophysical research communications》1967,29(3):281-287
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P R Chakraborty P Bandyopadhyay H H Huang U Maitra 《The Journal of biological chemistry》1974,249(21):6901-6909
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Quantitative analysis of DNA replication, in E. coli B cells infected by methyl methanesulfonate-treated bacteriophage T7, showed that production of phage DNA was delayed and decreased. The cause of the delay appeared to be a delay in host-DNA breakdown, the process which provides nucleotides for phage-DNA synthesis. In addition, reutilisation of host-derived nucleotides was impaired. These observations can be accounted for by a model in which methyl groups on phage DNA slow down DNA injection and also reduce the replicational template activity of the DNA once it has entered the cell. Repair of alkylated phage DNA may be required not only for replication but also for normal injection of DNA. 相似文献
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Initial steps in the large-scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase 总被引:2,自引:0,他引:2
W F Mangel 《Archives of biochemistry and biophysics》1974,163(1):172-177
Histones from exponential and stationary-phase mouse L-cells were quantitated after acrylamide gel electrophoresis in order to investigate cell cycle-dependent changes in the mode of binding of the various fractions in chromatin. By introducing various concentrations of citrate and divalent cations in the medium used for cell lysis and isolation of nuclei prior to histone extraction it was possible to demonstrate that certain histone fractions are preferentially retained in either exponential or stationary-phase nuclei. Differential retention of lysine-rich F1 was most evident when the lysing medium contained 1 mm Mg2+ and Ca2+ and 5 mm citrate (pH 2.75). In these conditions twice as much F1 is retained in stationary as in exponential nuclei. Differential retention of arginine-rich histones was most evident when the lysing medium contained 10 mm Mg2+ and Ca2+ and no citrate. In these conditions more F2a 1 is retained in exponential than in stationary nuclei while the opposite is true for F3. However, the total amount of arginine-rich fractions (F2a 1 + F3) retained was found to be the same in both cell phases. The results are discussed in relation to known structural features of the histones. 相似文献
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Altered bacteriophage T4 ribonucleic acid metabolism in a ribonuclease II-deficient mutant of Escherichia coli. 总被引:1,自引:1,他引:0
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Some early T4 ribonucleic acids were not found in an infected ribonuclease II-deficient strain but were formed in ribonuclease II+ transductants as well as in wild-type Escherichia coli. 相似文献