蝮蛇抗栓酶治疗血管性头痛14例

目前,治疗血管性头病尚无特效方法,我们采用蝮蛇抗栓酶治疗１４例,效果满意,现报告如下。１资料与方法１．１一般资料本组１４例,均为门诊病人,其中男性２例,女性１２例。年龄２３～５０岁,平均年龄４０岁,病程为６个月至７年。临床表现为头痛７例,偏头痛３例,...     

抢救海洛因急性中毒18例体会

我院１９９４年５月至１９９８年８月应用以纳络酮为主抢救海洛因中毒１８例,取得满意疗效,现报告如下。１临床资料１．１一般资料１８例中毒患者中男１６例,女２例,年龄１７～３１岁,平均２３岁。职业：无业青年１７例,职工１例。毒龄１～６年,中毒方式：１８例均...     

克痛宁配合中药治疗带状疱疹17例临床报告

作者应用克痛宁（云南下关制药厂）配合中药治疗带状疱疹１７例,疗效较好。现报告如下。１临床资料１．１一般资料治疗组１７例中,男性７例,女性１０例,年龄２９～６９岁,病程５～１８天。单纯累及躯干部７例,头面部２例,四肢部３例,损害较重累及躯干和四肢者５例...     

玉米两个RFLP标记的原位单杂交与共杂交定位的比较

ＲＦＬＰ标记ｂｎ１８．２３和 ｕｍｃ１１１位于玉米遗传日第 ４连锁群近端,彼此密切连锁但次序尚未确定。用生物素标记对它们进行了原位单杂交和共杂交的比较定位。在植物中,这类原位共杂交的研究为首次报道。在单一探针的原位杂交中 ｕｍｃ１１１被定位在第 １、 ４和９染色体长上,与着丝粒的百分距离分别是７．３６±２．６５、６３．６７±１．０７、４７．８７±２．９０。ｂｎ１８．２３被定位在第４和８染色体长臂上,与着丝粒的百分距离分别是８７．４２±２．４５和２７．６０±１．７５,清楚地表明了这两个标记在第４染色体上的次序。ｂｎ１８．２３和ｕｍｃ１１１分别与编码过氧化氢酶的ｃａｔ３基因和编码丝氨酸／苏氨酸蛋白激酶的ｃｄｅ２Ａ基因紧密连锁。根据供试ＲＦＬＰ标记检出位点推断了基因ｃｄｃ２Ａ和ｃａｔ３的物理位置。原位共杂交在第 ４染色体长臂上同时显示出了ｕｍｃ１１１和ｂｎ１８．２３两个标记的杂交信号,它们的位置分别与单一探针原位杂交的位置基本吻合。这为低拷贝或单一拷贝等小片段ＤＮＡ物理定位的可靠性以及它们共杂交的可行性提供了令人信服的证据。     

北京地区184例健康人肠道菌群值的调查

本文对北京地区１８４例７－６９岁健康人的粪便菌群中的肠杆菌、肠球菌、拟杆菌、产气荚膜梭菌、双歧杆菌、乳杆菌等６种细菌进行了培养、计数和鉴定。结果表明：肠杆菌６０７±１８０、肠球菌６１３±１４５、拟杆菌４５７±１７７、产气荚膜梭菌４０６±１５２、双歧杆菌８３２±０９６、乳杆菌７８２±０８６（ＬｏｇＮ／克便）;男性和女性相比,除产气荚膜梭菌男性明显高于女性（Ｐ＜００５）外,其余５种菌均无显著性差异;７－１８岁年龄组肠杆菌极显著地低于平均值（Ｐ＜００１）,６１—６９岁年龄组双歧杆菌明显低于平均值（Ｐ＜００５）;双歧杆菌与肠杆菌之比Ｂ／Ｅ值,７－１８岁年龄组是６１－６９岁年龄组的５７５倍     

阴道加德纳菌生物型与细菌性阴道病的关系

对１２０例细菌性阴道病患者和９０例正常对照组进行阴道加德纳菌的分离鉴定,同时采用Ｐｉｏｔ分型法对分离出的９３株Ｇｖ进行生物分型,结果显示ＢＶ患者Ｇｖ的检出率（５９．２％）明显高于正常对照组（２４．４％）,Ｐ〈０．０１。９３株Ｇｖ中,８个生物型全检测到,其中１型２３．７％,２型１８．３％,４型３２．３％,６型１０．８％,３型和５型均为５．４％,７型和８型为２．２％。ＢＶ患者主要为１、２、４型,显著高于正常对照组（８７．３％和３１．８％,Ｐ〈０．０１）。     

蕲蛇酶注射液治疗急性脑梗死Ⅱ期开放试验各医院分总结

蕲蛇酶注射液治疗急性脑梗死Ⅱ期开放试验各医院分总结北京医科大学第一附属医院７３例小结陈清棠１一般资料共７３例，男性５０例（６８．５％）、女性２３例（３１．５％），平均年龄（６３．１８±９．５１）岁。轻型４３例（５８．９％）、中型２４例（３２．９％）、...     

GL—7—ACA酰化酶在大肠杆菌中的分泌表达

利用来自假单胞菌的ＧＬ－７－ＡＣＡ酰化酶的信号肽和表达元件基因片段构建了ＧＬ－０７－ＡＣＡ酰化酶的分泌型高表达质粒ｐＴｒｃＣＡ１Ｓ和ｐＫＫＣＡ１Ｓ,其中ｐＴｒｃＣＡ１Ｓ为ＩＰＴＧ诱导型质粒,ｐＫＫＣＡ１Ｓ为组成型质粒。ｐＴｒｃＣＡ１Ｓ和ｐＫＫＣＡ１Ｓ转入受体菌ＴＧ１中都可高表达ＧＬ－７－ＡＣＡ酰化酶基因并将表达产物转运到周质空间,完整细胞酰楷酶比活力分别为２３．９单位每克菌体和１８．３单位每克菌体     

青龙肠溶胶囊和必可酮治疗支气管哮喘疗效观察

我们应用青龙肠溶胶囊、必可酮对１００例支气管哮喘病人治疗前后的肺功能和气道反应性进行了比较观察,现报告如下。１材料与方法１．１病例选择随机选择支气管哮喘患者１００例,其中男７２例,女２８例,年龄１４～６６岁,平均３４．７岁,病程最长２３年,最短２年,...     

原发性桥脑出血36例临床分析

我院１９９３～１９９７年共收治经头部ＣＴ确诊为桥脑出血３６例,现报告如下：１临床资料１．１一般资料本组３６例中男２３例,女１３例,年龄３２～７５岁,平均５５岁;其中３１～４０岁３例,４１～５０岁７例,５１～６０岁１７例,６１～７５岁９例。既往患高血压...     

西安市学生中从膝部长骨干骺融合推算身高与年龄的回归方程

本文根据西安市2612名学生的活体测量和1361例的X线拍片观察,得出了应用膝部长骨的干骺融合程度及某些线性度量推算身高与年龄的三十个回归方程和相应的误差,同时,对得出的方程进行了讨论。     

手腕部与膝部骨龄之间的差异

本文分别用Fels和RWT法评定了4902对2—17岁儿童青少年的手腕部和膝部骨龄。男女各年龄组骨龄的平均绝对差值为0.34—0.87岁,标准差为0.31—0.68岁。8—11岁前,这两个值往往随年龄增加;14岁前,男性一般大于女性。最大差值范围为1.45—2.99岁,其年龄变化往往不规律,但11岁前(男)和9岁前(女)常常增加。手腕部和膝部骨龄之间所存在的较大的绝对差值不能完全用观察误差加以解释。两部位的骨龄不能互相替代。所以,用骨龄预测成人身高和确定干骺固定术的时间,至少在5％的儿童中明显地受到骨龄部位的影响。     

Brief Communication: Timing of spheno‐occipital closure in modern Western Australians

The spheno‐occipital synchondrosis is a craniofacial growth centre between the occipital and sphenoid bones—its ossification persists into adolescence, which for the skeletal biologist, means it has potential application for estimating subadult age. Based on previous research the timing of spheno‐occipital fusion is widely variable between and within populations, with reports of complete fusion in individuals as young as 11 years of age and nonfusion in adults. The aim of this study is, therefore, to examine this structure in a mixed sex sample of Western Australian individuals that developmentally span late childhood to adulthood. The objective is to develop statistically quantified age estimation standards based on scoring the degree of spheno‐occipital fusion. The sample comprises multidetector computed tomography (MDCT) scans of 312 individuals (169 male; 143 female) between 5 and 25 years of age. Each MDCT scan is visualized in a standardized sagittal plane using three‐dimensional oblique multiplanar reformatting. Fusion status is scored according to a four‐stage system. Transition analysis is used to calculate age ranges for each defined stage and determine the mean age for transition between an unfused, fusing and fused status. The maximum likelihood estimates for the transition from open to fusing in the endocranial half is 14.44 years (male) and 11.42 years (female); transition from fusion in the ectocranial half to complete fusion is 16.16 years (male) and 13.62 years (female). This study affirms the potential value of assessing the degree of fusion in the spheno‐occipital synchondrosis as an indicator of skeletal age. Am J Phys Anthropol 153:132–138, 2014. © 2013 Wiley Periodicals, Inc.     

小鼠颌下腺内交感神经末梢变性时程的性差异

外源性神经生长因子(NGF)对周边交感系统的营养性影响已有广泛的研究(Black,1978;Purves等,1978)。但是,内源性NGF是否同样行使这种影响,至今还没有被人证实(Nja等,1978)。1979年,Carstairs等人利用丙酸睾酮慢性处理,提高小鼠颌下腺内NGF含量以后,观察到颈上神经节(SCG)中有部分神经元肥大,但其它交感神经节不受影响。作者认为SCG内肥大的神经元就是支配颌下腺的那部分交感神经元。由于雄性小鼠颌下腺内的NGF含量是雌性小鼠的92倍(Hirata等,1979),我们实验室直接检查了小鼠颌下腺和虹膜的交感神经支配,发现成年雄性小鼠颌下腺的荧光基丛索远较雌性小鼠的     

SEASONAL VARIATION IN SEXUAL SELECTION IN THE MOTTLED SCULPIN

Seasonal variation in sexual and natural selection in male mottled sculpins (Cottus bairdi) can be evaluated by calculating selection differentials, which measure the magnitude of phenotypic change resulting from selection, and by calculating indices of the opportunity for selection, which indicate the potential for phenotypic selection in a given interval. Selection differentials are high at the beginning of the breeding season and decline throughout the breeding season. The magnitude and direction of selection differentials depend on when spawning occurs and are independent of the size or age of the females that spawn. Annual selection differentials due to differences in mating success (female choice) are nearly constant between years. Annual selection differentials associated with hatching success are variable. Opportunities for selection (I = fitness variance/[mean fitness]²) show clear seasonal patterns. They are highest at the beginning and at the end of the spawning season. However, this variation is dependent on the mean used to calculate I, and hence variation in I values does not indicate a significant change in the variance of male fitness.     

FERTILIZATION IN PLUMBAGO ZEYLANICA: GAMETIC FUSION AND FATE OF THE MALE CYTOPLASM

Developmental phases surrounding the processes of gametic delivery and fusion were examined ultrastructurally in the reduced megagametophyte of Plumbago zeylanica, which lacks synergids. Gametic delivery occurs at the end of pollen tube growth and results in deposition of two male gametes, a vegetative nucleus, and a limited amount of pollen cytoplasm between the egg and central cell. Discharge of these materials from the tube is accompanied by loss of inner and outer pollen tube plasma membranes, loss of sperm-associated cell wall components, and disruption of the formerly continuous cell wall between the egg and central cell. The dispersion of egg cell wall components directly exposes female reproductive cell membranes to the unfused male gametes and pollen tube without disrupting gametic cell plasma membranes. Presence of unfused sperms within the female gametophyte appears to be a transitory phenomenon, lasting less than 5 min at the end of over 8½ hr of pollen tube growth. At the time of gametic deposition, plasma membranes of unfused sperm cells become directly appressed to plasma membranes of both the egg and central cell. Gametic fusion is initiated by a single fusion event between membranes of participating male and female cells, which is rapidly followed by subsequent, secondary fusion events between the same two cells at different locations along their surface. Gametic fusion results in the transmission of male gamete nuclei with co-transmission of nearly the entire sperm cytoplasmic volume and organellar complement, and it is possible to identify heritable male cytoplasmic organelles within both the incipient zygote and endosperm. Paternally originating plastids may be distinguished from maternal plastids by differences in morphology and staining characteristics, whereas paternal mitochondria may be distinguished from maternal mitochondria by populational differences in mitochondrial size which are statistically significant. Such observations further indicate that transmitted paternal mitochondria seem to remain viable, as judged by their ultrastructural appearance, and are transmitted exclusively by sperm cytoplasm rather than discharged pollen cytoplasm. The presence of anucleate, membrane-bounded cytoplasmic bodies between the egg and central cell are identifiable on the basis of their enclosed organelles and indicate that fragmentation of a small amount of the sperm cytoplasm associated with the vegetative nucleus commonly occurs. The presence and identification of sperm cytoplasmic organelles and associated membranes within female reproductive cells following gametic transmission represents strong evidence in support of the cellular basis of nuclear and cytoplasmic transmission during sexual reproduction in Plumbago.     

Time of fusion of the human sternebrae with one another in northwest India

The time of fusion of the sternebrae with one another was studied in sterna obtained from cadavers of 806 male and 212 female adults and 66 male and 29 female children, on whom medicolegal postmortem examinations had been conducted by the senior author. Sterna of 31 neonates were also studied. The bones were X-rayed in an anteroposterior view and later macerated, cleaned, and dried. The sternebrae fused with one another from below upwards. In both sexes, the third and fourth sternebrae showed partial or commencement of fusion in a majority of specimens in the 0-5 years age group. Although the earliest complete fusion was seen in the 6-10 years age group, 100% specimens exhibited complete fusion in the 15-17 years age group. The earliest complete fusion between second and third sternebrae was observed in the 11-14 years age group in both sexes; it was seen in 100% of specimens in males over 25 years and in females over 30 years. The first and second segments showed earliest complete fusion in a boy of 16 years and in a girl of 18 years; 100% of specimens showed complete fusion in the males over 60 years and in the females over 30 years. A mesosternum showing complete fusion of all the sternebrae must be from a subject over 16 years of age if male and over 18 years if female. These observations are at variance with those of workers in Western countries. There has been no other report from India on this subject.     

西安市学生膝部长骨的干骺融合时间与身高等发育指标的关系

本文根据西安市2612例学生的活体测量以及电子计算机对数据的统计分析,得出了西安市学生身高等发育指标及有关指数,绘制了有关的发育曲线,讨论了身高等发育指标与膝部长骨干骺融合的关系。     

男性青少年脂肪组织分布与相对骨龄的关系——Fels追踪研究

本文对Fels追踪研究中8—17岁男性青少年的相对骨龄与脂肪分布类型之间的关系做了分析。按体重/身高~2调整后,如用每个年龄的三种皮褶厚度(ST)指数的均值表示脂肪分布类型的话,8—12岁时,脂肪分布类型呈外周型分布,但13岁后开始朝向心型发展呈全身性分布。如用肩胛下ST/(肩胛下ST+肱三头肌区ST)的比例表示的话,那么14—17岁时,相对骨龄早者(简称早组)与相对骨龄晚者(简称晚组)相比,前者有较明显的向心型分布倾向。13—14岁时,早组的上述比值的年增长明显大于晚组。但是,按脂肪分布类型指数等级的基线和体重/身高~2调整之后,7、11或14岁时的相对骨龄不能预测17岁时的脂肪分布类型指数的等级。所以,我们可以得出这样的结论:如按本文的比例指数加以定量的话,脂肪分布类型与男性青少年的相对骨龄只有微弱的关系。他们的脂肪分布类型可能与其它成熟指征(如男性青春期的第二性征)有明显的关系。