Caring for older people. Carers.

Caring by families and friends is the backbone of community care. Carers face physical, emotional, social, and financial problems. They need recognition, information, and support from the health professionals with whom they and the person they care for come in contact. Much information is available to assist carers and to enable their doctors to help them in their caring role.     

Analytical techniques for cell fractions. XXIV. Isoelectric point stadnards for two-dimensional electrophoresis.

We have investigated the two-dimensional electrophoretic behavior of a number of proteins throughout the course of carbamylation induced by heating in urea solution. Stepwise charge changes occur which are apparently commensurate with the number of free amino groups in the protein. Characteristic changes also occur in the sodium dodecyl sulfate (SDS) electrophoretic mobility (SDS molecular weight) of some heavily carbamylated proteins, implying altered structure or SDS binding. A mixture of all carbamylation intermediates of a protein provides a simple solution to the problem of internal isoelectric point standardization in the two-dimensional electrophoretic technique of P. H. O'Farrell (1975, J. Biol. Chem.250, 4007–4021).