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1.
G E Kaurov YuNAksyonova E R Lovyagina I I Ivanov A B Rubin 《General physiology and biophysics》1992,11(3):229-239
Stationary delayed fluorescence (DF) of chlorophyll in isolated membrane preparations from thermophilic cyanobacterium Synechococcus elongatus was investigated as a function of temperature. Two peaks at different temperatures were observed. The low-temperature peak (54-60 degrees C) coincided with the main maximum of the thermally-induced delayed fluorescence of chlorophyll in intact cells and PSII-particles with active oxygen-evolving system. The high-temperature peak (78 degrees C) coincided with the minor band of delayed light emitted by intact cells. It was also observed in the delayed fluorescence emission from a PSI-enriched fraction preparation. The intensities of the DF peaks were dependent on the presence of inhibitors, donors and acceptors that cause specific effects on electron transport of the two photosystems. The low-temperature and high-temperature peaks were related to PSII and PSI, respectively. The manifestation of delayed fluorescence from PSI and PSII at different temperatures seems to be a specific property of thermophilic cyanobacteria. The reason for this may be a high thermal stability of the photosystems and the lack of the PSII antenna complex in isolated membranes. Consequently, the relative yield of delayed fluorescence from PSI markedly increases. Thermally-induced fluorescence seen in membranes of cyanobacteria showed a high sensitivity to structural and functional membrane alterations induced by pH changes, different electron transport stabilizing agents or different concentrations of MgCl2. 相似文献
2.
Differences in the temperature dependence and thermosensitivities of PSII activities in Synechocystis sp. PCC6803 grown at 25 and 35 degrees C were studied. Hill reactions in cells, thylakoid membranes and purified PSII core complexes were measured at high temperatures or at their growth temperatures after high-temperature treatments. In the presence of 2,5-dichloro-p-benzoquinone as an electron acceptor, which can accept electrons directly from Q(A), the temperature dependence of the oxygen-evolving activity was almost the same in thylakoid membranes and in the purified PSII complexes from cells grown at 25 or 35 degrees C. When duroquinone, which accepts electrons only through Q(B) plastoquinone, was used as an electron acceptor, the temperature dependence was the same for purified PSII core complexes but was different between thylakoids isolated from the cells grown at 25 and 35 degrees C. No remarkable difference was observed in protein compositions between thylakoids and between purified PSII complexes from cells grown at 25 or 35 degrees C. However, the fluidity of thylakoids, measured by electron flow to P700, was affected by the growth temperature. These results suggest that one of the major factors which cause the changes in the thermosensitivity of PSII is the change in the fluidity of thylakoid membranes. As for the acclimation of PSII in thylakoids to high temperatures, one of the main causes is the decrease in the high-temperature-induced formation of non-Q(B) PSII due to the decreased fluidity in the cells grown at 35 degrees C. 相似文献
3.
In a study of the responses of photosystem II (PSII) to high temperature in suspension-cultured cells of soybean (Glycine max L. Merr.), we found that high temperatures inactivated PSII via two distinct pathways. Inactivation of PSII by moderately
high temperatures, such as 41°C, was reversed upon transfer of cells to 25°C. The recovery of PSII required light, but not
the synthesis of proteins de novo. By contrast, temperatures higher than 45°C inactivated PSII irreversibly. An increase in
the growth temperature from 25 to 35°C resulted in an upward shift of 3°C in the profile of the heat-induced inactivation
of PSII, which indicated that the thermal stability of PSII had been enhanced. This acclimative response was reflected by
the properties of isolated thylakoid membranes: PSII in thylakoid membranes from cells that had been grown at 35°C exhibited
greater thermal stability than that from cells grown at 25°C. Disruption of the vesicular structure of thylakoid membranes
with 0.05% Triton X-100 decreased the thermal stability of PSII to a similar level in both types of thylakoid membrane. Proteins
released by Triton X-100 from thylakoid membranes from cells grown at 35°C were able to increase the thermal stability of
Triton-treated thylakoid membranes. These observations suggest that proteins that are associated with thylakoid membranes
might be involved in the enhancement of the thermal stability of PSII. 相似文献
4.
Variable fluorescence (Fv), i.e., Fv = Fm-Fo where Fo is the minimal fluorescence and Fm the maximum fluorescence, and difference Fourier transform infrared (FT-IR) spectroscopy were used to study the effect of heat stress in the 25-55 degrees C range on photosystem II (PSII) structure and function. First, the Fv intensity reflects accurately the changes in the number of open photochemical centers in PSII. Secondly, the use of Fv in combination with FT-IR spectroscopy can disclose structure-function correlations in the heat inactivation of the PSII complex. Analysis of the midpoint temperatures of thermal denaturation, i.e., 50% inactivation, reported so far in investigations of the thylakoid membrane components has revealed that most of the thermal transitions attributed to PSII are in the 39-46 degrees C range. In this work, it is shown specifically that the midpoint temperature of PSII inactivation is at about 40 degrees C. Moreover, it was clearly demonstrated that the heat-induced changes above 40 degrees C are the result of a marked decrease in the number of open photochemical centers in PSII. It was also seen that above this same temperature the loss of photochemical centers has its structural counterpart in overall modifications of the secondary structures of the PSII proteins resulting from the decrease in the alpha-helix content concomitant with the increase in extended chain (beta-strand) conformations. In brief, a novel finding reported here is that the number of open photochemical centers in PSII is dependent on a dynamic equilibrium between the contents of the PSII proteins in alpha-helix and extended chains (beta-strands), but not in beta-sheets and beta-turn structures except for the antiparallel-beta-sheet conformations. This therefore associates the thermal inactivation of the photochemical centers in photosystem II with distinct conformational changes in the proteins of the PSII supramolecular complex. In the particular context of the present study, these findings constitute a significant contribution to the investigation of structure-function correlations in the photosynthetic membrane. In a broader context, this information might be essential for the comprehension of the molecular arrangements or local structure order that are involved directly or indirectly in biological catalysis. 相似文献
5.
Effects of high temperatures on the photosynthetic systems in spinach: Oxygen-evolving activities,fluorescence characteristics and the denaturation process 总被引:16,自引:0,他引:16
Yamane Yoshihiro Kashino Yasuhiro Koike Hiroyuki Satoh Kazuhiko 《Photosynthesis research》1998,57(1):51-59
Activities of oxygen evolution, fluorescence Fv (a variable part of chlorophyll fluorescence) values, and amounts of the 33 kDa protein remaining bound to the thylakoids in intact spinach chloroplasts were measured during and after high-temperature treatment. The following results were obtained. (1) Both the Fv value and the flash-induced oxygen evolution measured by an oxygen electrode were decreased at high temperatures, but they showed partial recovery when the samples were cooled down and incubated at 25°C for 5 min after high-temperature treatment. (2) Oxygen evolution was more sensitive to high temperatures than the Fv value, and the decrease in the Fv/Fm ratio at high temperatures rather corresponded to that in the oxygen evolution measured at 25°C after high-temperature treatment. (3) Photoinactivation of PS II was very rapid at high temperatures, and this seems to be a cause of the difference between the Fv values and the oxygen-evolving activities at high temperatures. (4) At around 40°C, the manganese-stabilizing 33 kDa protein of PS II was supposed to be released from the PS II core complexes during heat treatment and to rebind to the complexes when the samples were cooled down to 25°C. (5) At higher temperatures, the charge separation reaction of PS II was inactivated, and the PS II complexes became less fluorescent, which was recovered partially at 25°C. (6) Increases in the Fv value due to a large decrease in the electron flow from QA to QB became prominent after high-temperature treatment at around 50°C. This was the main cause of the discrepancy between the Fv values and the oxygen-evolving activities measured at 25°C. Relationship between the process of heat inactivation of PS II reaction center complexes and the fluorescence levels is discussed. 相似文献
6.
Heat stress induces an aggregation of the light-harvesting complex of photosystem II in spinach plants
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Whole spinach (Spinacia oleracea) plants were subjected to heat stress (25 degrees C-50 degrees C) in the dark for 30 min. At temperatures higher than 35 degrees C, CO2 assimilation rate decreased significantly. The maximal efficiency of photosystem II (PSII) photochemistry remained unchanged until 45 degrees C and decreased only slightly at 50 degrees C. Nonphotochemical quenching increased significantly either in the absence or presence of dithiothreitol. There was an appearance of the characteristic band at around 698 nm in 77 K fluorescence emission spectra of leaves. Native green gel of thylakoid membranes isolated immediately from heat-stressed leaves showed that many pigment-protein complexes remained aggregated in the stacking gel. The analyses of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting demonstrated that the aggregates were composed of the main light-harvesting complex of PSII (LHCIIb). To characterize the aggregates, isolated PSII core complexes were incubated at 25 degrees C to 50 degrees C in the dark for 10 min. At temperatures over 35 degrees C, many pigment-protein complexes remained aggregated in the stacking gel of native green gel, and immunoblotting analyses showed that the aggregates were composed of LHCIIb. In addition, isolated LHCII was also incubated at 25 degrees C to 50 degrees C in the dark for 10 min. LHCII remained aggregated in the stacking gel of native green gel at temperatures over 35 degrees C. Massive aggregation of LHCII was clearly observed by using microscope images, which was accompanied by a significant increase in fluorescence quenching. There was a linear relationship between the formation of LHCII aggregates and nonphotochemical quenching in vivo. The results in this study suggest that LHCII aggregates may represent a protective mechanism to dissipate excess excitation energy in heat-stressed plants. 相似文献
7.
Georgieva K Maslenkova L 《Zeitschrift für Naturforschung. C, Journal of biosciences》2006,61(3-4):234-240
The stability of PSII in leaves of the resurrection plant Haberlea rhodopensis to high temperature and high light intensities was studied by means of chlorophyll fluorescence measurements. The photochemical efficiency of PSII in well-hydrated Haberlea leaves was not significantly influenced by temperatures up to 40 degrees C. Fo reached a maximum at 50 degrees C, which is connected with blocking of electron transport in reaction center II. The intrinsic efficiency of PSII photochemistry, monitored as Fv/Fm was less vulnerable to heat stress than the quantum yield of PSII electron transport under illumination (phiPSII). The reduction of phiPSII values was mainly due to a decrease in the proportion of open PSII centers (qP). Haberlea rhodopensis was very sensitive to photoinhibition. The light intensity of 120 micromol m(-2) s(-1) sharply decreased the quantum yield of PSII photochemistry and it was almost fully inhibited at 350 micromol m(-2) s(-1). As could be expected decreased photochemical efficiency of PSII was accompanied by increased proportion of thermal energy dissipation, which is considered as a protective effect regulating the light energy distribution in PSII. When differentiating between the three components of qN it was evident that the energy-dependent quenching, qE, was prevailing over photoinhibitory quenching, qI, and the quenching related to state 1-state 2 transitions, qT, at all light intensities at 25 degrees C. However, the qE values declined with increasing temperature and light intensities. The qI was higher than qE at 40 degrees C and it was the major part of qN at 45 degrees C, indicating a progressing photoinhibition of the photosynthetic apparatus. 相似文献
8.
N Inoue Y Taira T Emi Y Yamane Y Kashino H Koike K Satoh 《Plant & cell physiology》2001,42(10):1140-1148
High-temperature effects on Photosystem II and plasma membranes, temperature dependence of growth, and acclimation to the growth temperature were studied in a mesophilic cyanobacterium, Synechocystis sp. PCC6803. The following results were obtained. (1) Small but distinct temperature acclimation of the PSII reaction center activity was shown for the first time when the activity was measured at inhibitory high temperatures. However, the reaction center activity showed no apparent acclimation when it was measured at growth temperatures after heat stress. (2) Oxygen-evolving activity and the permeability of plasma membranes showed higher resistance to heat when PCC6803 cells were grown at higher temperatures. (3) Acclimation of photosynthesis to the growth temperature seemed to occur so as to maintain photosynthesis activity not at a maximum level but in a certain range at the growth temperatures. (4) Neither sensitivity to high-temperature-induced dissociation of phycobilisomes from the PSII reaction center complexes nor degradation of phycocyanin were altered by changes in the environmental temperature. (5) A close relationship between the viability of cells and the structural changes of plasma membranes (but not the inactivation of photosynthesis) was observed. The denaturation process of PSII complexes and the relationship between the temperature dependence of the growth of Synechocystis PCC6803 cells and that of the photosynthetic activity are also discussed. 相似文献
9.
Mas-Oliva J Delgado-Coello B 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2007,146(1-2):207-213
Cholesterol has been shown to regulate the activity of several membrane proteins. Although this phenomenon represents an important factor in the regulation of ion homeostasis, insights are needed to fully understand the role of this lipid in cell function in order to better comprehend the effect of bilayer components upon membrane function. Since evolution has shaped the composition of the membrane bilayer, it becomes of interest to study these changes in parallel with the many functions of membranes such as ion transport. The present study employing a plasma membrane preparation obtained from calf ventricular muscle demonstrates that cholesterol partially inhibits the Ca(2+),Mg(2+)-ATPase as the catalytic function of the calcium pump, when incubation reaction temperatures are below 42 degrees C. In contrast, when incubation reaction temperatures are above 42 degrees C, cholesterol apparently promotes enzyme stabilization reflected in higher activity. Although the activation energy values for the enzyme are almost the same at ranges between 15 and 40 degrees C, the use of elevated temperatures promote higher enzyme inactivation rates in control than in cholesterol enriched membranes. Cholesterol apparently is promoting stabilization that in turn protects the enzyme against thermal inactivation. This protective effect is reflected in a decrease of inactivation rate values and energy released during enzyme catalysis. The modification of many membrane properties throughout million of years made it possible for new evolutionary driving forces to show themselves as new characteristics in eukaryotes such as the one discussed in this study, dealing with the presence of cholesterol in the cell membrane directly associated to the promotion of protein thermostability. 相似文献
10.
Membrane lipid unsaturation modulates processing of the photosystem II reaction-center protein D1 at low temperatures. 总被引:1,自引:0,他引:1
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The role of membrane lipid unsaturation in the restoration of photosystem II (PSII) function and in the synthesis of the D1 protein at different temperatures after photoinhibition was studied in wild-type cells and a mutant of Synechocystis sp. PCC 6803 with genetically inactivated desaturase genes. We show that posttranslational carboxyl-terminal processing of the precursor form of the D1 protein is an extremely sensitive reaction in the PSII repair cycle and is readily affected by low temperatures. Furthermore, the threshold temperature at which perturbations in D1-protein processing start to emerge is specifically dependent on the extent of thylakoid membrane lipid unsaturation, as indicated by comparison of wild-type cells with the mutant defective in desaturation of 18:1 fatty acids of thylakoid membranes. When the temperature was decreased from 33 degrees C (growth temperature) to 18 degrees C, the inability of the fatty acid mutant to recover from photoinhibition was accompanied by a failure to process the newly synthesized D1 protein, which accumulated in considerable amounts as an unprocessed precursor D1 protein. Precursor D1 integrated into PSII monomer and dimer complexes even at low temperatures, but no activation of oxygen evolution occurred in these complexes in mutant cells defective in fatty acid unsaturation. 相似文献
11.
Most plants growing in temperate desert zone exhibit brief temperature-induced inhibition of photosynthesis at midday in the
summer. Heat stress has been suggested to restrain the photosynthesis of desert plants like Alhagi sparsifolia S. It is therefore possible that high midday temperatures damage photosynthetic tissues, leading to the observed inhibition
of photosynthesis. In this study, we investigated the mechanisms underlying heat-induced inhibition of photosynthesis in A. sparsifolia, a dominant species found at the transition zone between oasis and sandy desert on the southern fringe of the Taklamakan
desert. The chlorophyll (Chl) a fluorescence induction kinetics and CO2 response curves were used to analyze the thermodynamic characters of both photosystem II (PSII) and Rubisco after leaves
were exposed to heat stress. When the leaves were heated to temperatures below 43°C, the initial fluorescence of the dark-adapted
state (Fo), and the maximum photochemical efficiency of PSII (Fv/Fm), the number of active reaction centers per cross section (RCs) and the leaf vitality index (PI) increased or declined moderately.
These responses were reversed, however, upon cooling. Moreover, the energy allocation in PSII remained stable. The gradual
appearance of a K point in the fluorescence curve at 48°C indicated that higher temperatures strongly impaired PSII and caused
irreversible damage. As the leaf temperature increased, the activity of Rubisco first increased to a maximum at 34°C and then
decreased as the temperature rose higher. Under high-temperature stress, cell began to accumulate oxidative species, including
ammoniacal nitrogen, hydrogen peroxide (H2O2), and superoxide (O2
·−), suggesting that disruption of photosynthesis may result from oxidative damage to photosynthetic proteins and thylakoid
membranes. Under heat stress, the biosynthesis of nonenzyme radical scavenging carotenoids (Cars) increased. We suggest that
although elevated temperature affects the heat-sensitive components comprising of PSII and Rubisco, under moderately high
temperature the decrease in photosynthesis is mostly due to inactivation of dark reactions. 相似文献
12.
Allakhverdiev SI Los DA Mohanty P Nishiyama Y Murata N 《Biochimica et biophysica acta》2007,1767(12):1363-1371
Transformation with the bacterial gene codA for choline oxidase allows Synechococcus sp. PCC 7942 cells to accumulate glycinebetaine when choline is supplemented exogenously. First, we observed two types of protective effect of glycinebetaine against heat-induced inactivation of photosystem II (PSII) in darkness; the codA transgene shifted the temperature range of inactivation of the oxygen-evolving complex from 40-52 degrees C (with half inactivation at 46 degrees C) to 46-60 degrees C (with half inactivation at 54 degrees C) and that of the photochemical reaction center from 44-55 degrees C (with half inactivation at 51 degrees C) to 52-63 degrees C (with half inactivation at 58 degrees C). However, in light, PSII was more sensitive to heat stress; when moderate heat stress, such as 40 degrees C, was combined with light stress, PSII was rapidly inactivated, although these stresses, when applied separately, did not inactivate either the oxygen-evolving complex or the photochemical reaction center. Further our studies demonstrated that the moderate heat stress inhibited the repair of PSII during photoinhibition at the site of synthesis de novo of the D1 protein but did not accelerate the photodamage directly. The codA transgene and, thus, the accumulation of glycinebetaine alleviated such an inhibitory effect of moderate heat stress on the repair of PSII by accelerating the synthesis of the D1 protein. We propose a hypothetical scheme for the cyanobacterial photosynthesis that moderate heat stress inhibits the translation machinery and glycinebetaine protects it against the heat-induced inactivation. 相似文献
13.
Protection against the photo-induced inactivation of the photosystem II complex by abscisic acid 总被引:2,自引:1,他引:1
P. Pardha Saradhi I. Suzuki A. Katoh A. Sakamoto P. Sharmila D.-J. Shi & N. Murata 《Plant, cell & environment》2000,23(7):711-718
To examine the effect of abscisic acid (ABA) on the photo‐induced inactivation of the photosystem II (PSII) complex, a suspension culture of Chlamydomonas reinhardtii was treated with ABA for 24 h in darkness and then, after removal of ABA, the cells were exposed to strong light at a photon flux density of 2000 μ mol m ? 2 s ? 1 at various temperatures. The activity of PSII, as estimated in terms of chlorophyll fluorescence and the evolution of oxygen, decreased significantly during the exposure of cells to the strong light, and the extent of the photo‐induced decrease in PSII activity was much greater at lower temperatures. Irrespective of temperature, the decrease in PSII activity in ABA‐treated cells was significantly smaller than that in control cells. Moreover, the recovery of PSII activity from the photo‐inactivated state in ABA‐treated cells was significantly faster than that in control cells. The recovery of PSII activity in both ABA‐treated and control cells was almost entirely prevented by the presence of chloramphenicol. These results indicate that ABA protects the PSII complex in C. reinhardtii against photo‐induced inactivation by accelerating the recovery of this complex. 相似文献
14.
高温胁迫对柑橘光合速率和光系统Ⅱ活性的影响 总被引:35,自引:12,他引:35
用红外CO2分析仪和叶绿素荧光仪测定了温州蜜柑和脐橙叶片的净光合速率(Pn)、初始荧光(Fo)、最大光能转换效率(Fv/Fm)及电子传递速率(ETR).结果表明,与常温(25℃)相比,高温胁迫(38~40℃)使温州蜜柑和脐橙叶片的Pn、Fv/Fm及ETR下降,Fo升高.胁迫25d后温州蜜柑和脐橙叶片的Pn分别下降55.6%和39.8%.Fv/Fm下降22.0%和6.7%,ETR下降55.0%和41.5%,Fo分别上升了113.8%和14.9%.柑橘经高温胁迫后,在25℃下处理10d,叶片的Pn、Fv/Fm、Fo及ETR恢复明显.这些结果说明柑橘的光合速率下降与PSⅡ反应中心失活有关. 相似文献
15.
A study of heat effects was performed in thylakoids and photosystem II (PSII)-enriched membranes isolated from spinach in
relation to Cl−-induced activation of PSII catalyzed oxygen evolution and the retention of Cl− in the PSII complex. For this, Cl−-sufficient membranes and low-Cl− membranes were used. The presence of Cl− in the reaction medium did accelerate oxygen evolution, which remained unaffected by heat treatment up to 40°C in PSII membranes
and up to 42.5°C in thylakoids. Heat resistance of Cl−-induced activation of oxygen evolution was found to be independent of the presence of ‘bound Cl−’ in the preparations. However, the functional stability of the PSII complex during heat treatment showed a marked dependence
on the presence of bound Cl− in PSII. Electron paramagnetic resonance study of manganese (Mn) release per reaction center/YD+ showed that there was little loss of Mn2+ up to 42°C in our preparations, although the PSII activity was significantly lowered. These observations together with data
from steady state chlorophyll a fluorescence imply that the site of action of Cl− causing direct activation of oxygen evolution was different from the site of primary heat damage. A differential response
of chloride binding sites to heat stress was observed. The high-affinity (tightly bound, slow exchanging) site of chloride
is affected earlier (∼37°C) while low-affinity (loosely bound, fast exchanging) site gets affected at higher temperatures
(42.5°C in thylakoids and 40°C in the case of PSII-enriched membranes).
Prasanna Mohanty is an INSA Honorary Scientist and Professor on Courtesy, DAVV, Indore. 相似文献
16.
The carboxyl terminus of the CP43 subunit of photosystem II (PSII) in the thermophilic cyanobacterium, Synechococcus elongatus, was genetically tagged with six consecutive histidine residues to create a metal binding site on the PSII supramolecular complex. The histidine-tagging enabled rapid isolation of an intact cyanobacterial PSII core complex from dodecyl maltoside-solubilized thylakoids by a simple one-step Ni(2+)-affinity column chromatography. The isolated core complex was in a dimeric form with a molecular mass of about 580 kDa, consisting of five major intrinsic membrane proteins (CP47, CP43, D1, D2 and cytochrome b-559), three extrinsic proteins (33 kDa, 12 kDa, and cytochrome c-550), and a few low molecular mass membrane proteins, and evolved oxygen at a rate as high as 3,400 mumol (mg Chl)-1 h-1 at 45 degrees C with ferricyanide as an electron acceptor. The core complex emitted thermoluminescence B2-, B1- and Q-bands arising from S2QB-, S3QB- and S2QA- charge recombinations at respective emission temperatures of 45, 38 and 20 degrees C, all of which were higher by about 15 degrees C as compared with those in mesophilic spinach BBY membranes. These results indicated that the isolated core complex well retained the intact properties of thermoluminescence of thermophilic cyanobacterial cells, the deeper stabilization of PSII charge pairs. The isolated complex was extremely stable in terms of both protein composition and function, exhibiting no release of extrinsic proteins, no proteolytic degradation in any of its subunits, accompanied by only a slight (less than 10%) loss in oxygen evolution, after dark-incubation at 20 degrees C for 8 d. These properties of the thermophilic PSII core complex are highly useful for various types of studies on PSII. 相似文献
17.
The temperature dependence of various activities related to the energy metabolism of isolated membranes and whole cells of the thermophilic bacterium Chloroflexus aurantiacus was determined after phototrophic growth at either 40, 50, or 60 degrees C. The data obtained were expressed by use of Arrhenius plots. Maximum activities were determined at about 65 degrees C for succinate 2,4-dichlorophenol-indophenol reductase as well as NADH oxidase and at about 70 degrees C for Mg-ATPase and for light-induced proton extrusion by cells. Activation energies for Mg-ATPase and light-induced proton extrusion were about 40 kJ mol-1 from 30 degrees C to about 50 degrees C and they increased significantly at higher temperatures. Essentially the same dependency was detectable with NADH oxidase, except for an increase in activation energy below 41 degrees C. All of these responses were independent of growth temperature. Succinate-2,4-dichlorophenol-indophenol reductase showed a change in activation energy around 41 degrees C only with cells grown at 60 degrees C. Differences in the responses of cells grown at different temperatures were identified on the basis of changes from sigmoidal to hyperbolic kinetics for light saturation of proton extrusion. Moreover, the thermostability of proton extrusion was maximal when assayed at the corresponding growth temperatures. In any case, thermostability was lowest at the 65 and 68 degrees C assay temperatures. Differential scanning calorimetry with membranes revealed irreversible heat uptake from about 60 to 72 degrees C. The results are discussed in light of the activation energy for the specific growth rate, which is lowest at temperatures from 40 degrees C to the optimum at 60 degrees C. 相似文献
18.
A shift of the growth temperature from 40 degrees C to 18 degrees C promoted an increase in the degree of fatty acids unsaturation and a decrease, from 26 degrees C to 0 degrees C, of the phase transition temperature of thylakoid membranes in Anabaena siamensis. The pattern of photoinhibition of photosynthesis at distinct temperatures varied as a function of the phase transition temperature. In the absence of streptomycin, a pronounced photoinhibition at temperatures near the phase transition (26 degrees C) was observed in cells grown at 40 degrees C, while protection from photodamage was observed at chilling temperatures (15 degrees C to 5 degrees C). In this same range of temperature, such a protection was not verified if cells were grown at 18 degrees C. In both types of cells, however, the rate of photoinactivation in the presence of streptomycin was progressively decreased by lowering the temperature of photoinhibition. When recovery from photoinhibition was followed at the respective temperature in which cells were grown, the restoration profile of the photosynthetic O(2) evolution to initial levels was essentially the same in both types of cells. The protective effect of low temperatures against photoinhibition was attributed to a decreased solubility and diffusion of oxygen in the thylakoid membranes due to an increase of the membrane viscosity that would avoid the photogeneration of reactive oxygen species around PS II. 相似文献
19.
J Kruuv D Glofcheski K H Cheng S D Campbell H M Al-Qysi W T Nolan J R Lepock 《Journal of cellular physiology》1983,115(2):179-185
The Arrhenius plot of the rate of V79 Chinese hamster cell inactivation due to hypothermia has a "break" around 7-10 degrees C with optimum storage temperature for unprotected cells being about 10 degrees C. Addition of the membrane lipid perturber, butylated hydroxytoluene, improves survival of cells when compared to controls at temperatures below this break but not above. Arrhenius plots of growth rates of the cells show breaks at 30 and 40 degrees C. Measurements of membrane fluidity by electron spin resonance or membrane polarization anisotropy by fluorescence spectrophotometry techniques as a function of temperature in these cells also reveal "breaks" centered around 8 and 30 degrees C. Hence, the changes in the rate of cell inactivation and growth as a function of temperature may be related to membrane lipid phase changes. 相似文献
20.
Effects of prolonged freezing stress on the photosynthetic apparatus of moderately hardy leaves as assayed by chlorophyll fluorescence kinetics 总被引:1,自引:0,他引:1
Abstract Moderately frost-hardy leaves of the wintergreen broadleaf woody shrubs Pyracantha coccinea and Ligustrum ovalifolium and the winter annual herb Spinacia oleracea were subjected to extended freezing stress up to 15 d at temperatures 2–8°C above the mean lethal temperature (LT50). After thawing, the fast kinetics of in vivo chlorophyll fluorescence of photosystem II (PSII) and the potential of linear photosynthetic electron transport of isolated thylakoid membranes was measured at room temperature. The lower the minimum freezing temperature and the longer the time of exposure, the greater was the suppression of the fluorescence signals of the leaves and decrease of the electron transport capacity of the thylakoid membranes. The pattern of inactivation of PSII -mediated electron flow, i.e. inhibition of photoreaction to photochemistry and/or electron donation to the photochemical reaction, during long-term freezing at temperatures somewhat above the LT50 of the leaves was similar to that observed earlier after relatively brief exposure of leaves and isolated thylakoid membranes to more severe freezing stress. As injury occurred during freezing in complete darkness, it is likely that prolonged winter stress under natural environmental conditions causes changes in the photosynthetic apparatus of moderately hardy leaves which are not due to photoinhibition. 相似文献