湖北、河南、安徽三省大别山区地理新分布植物

湖北、河南、安徽三省大别山区地理新分布植物何家庆（安徽大学生物系合肥２３００３９）关键词大别山区,种子植物,地理新分布ＴＨＥＮＥＷＧＥＯＧＲＡＰＨＩＣＡＬＤＩＳＴＲＩＢＵＴＩＯＮＯＦＳＰＥＲＭＡＴＯＰＨＹＴＥＩＮＤＡＢＩＥＳＨＡＮＴＨＥＲＥＧＩＯＮＳ...     

UV—B辐射对小麦叶片H2O2代谢的影响

研究了温室种植的小麦在０（ＣＫ）、８．８２ｋＪ／ｍ＾２（Ｔ１）和１２．６ｋＪ／ｍ＾２（Ｔ２）三种剂量的紫外线Ｂ（ＵＶ－Ｂ）辐射下Ｈ２Ｏ２含量的变化及其机理。ＵＶ－Ｂ辐射下Ｈ２Ｏ２、还原型抗坏血酸（ＡｓＡ）和还原型谷胱甘肽（ＧＳＨ）含量增加,抗坏血酸过氧化物酶（ＡＰｘ）和谷胱甘肽不原酶（ＧＲ）活性升高,脂肪酸不饱和度指数（ＩＵＦＡ）降低。ＳＤＳ－ＰＡＧＥ谱图没有质上的差异,但凝胶着色深浅有变化。分析     

付里叶变换红外光谱研究短杆菌肽A及其在脂双层中加Na~＋前后的构象变化

用ＦＴＩＲ光谱研究了短杆菌肽Ａ（ＧＡ）在固态,组装入ＤＰＰＣ脂持体,以及Ｎａ＾＋结合状态的构象变化。固态时酰胺Ｉ带峰位于１６３３ｃｍ＾－１左右,且在１６８５ｃｍ＾－１处有一肩峰;酰胺Ⅱ带位于１５３０ｃｍ＾１左右,整个分子为反平行的β螺旋结构,为非通道构象。当ＧＡ组装入脂质体后,酰胺Ｉ带仍位于１６３３ｃｍ＾－１,但１６８５ｃｍ＾－１的肩峰消失,酰胺Ⅱ带移至１５５０ｃｍ＾－１,分子间氢键转变为分子内氢     

BmK AS在大鼠脑和蟑螂神经索突触体上的药理结合特性

用ＩＮＤＯＧＥＮ法对一个新型东亚钳蝎活性多肽ＢｍＫ ＡＳ进行了＾１２５Ｉ标记。分别在哺乳动物和昆虫标本上观察了标记物的药理结合特性,结果表明ＢｍＫ ＡＳ大大鼠脑突触体标本上有单一非协同结合位点,其平衡解离常数Ｋｄ与最大结合容量Ｂｍａｘ分别为１．４９ｎｍｏｌ／Ｌ,１．３９ｎｍｏｌ／ｇ。此外,ＢｍＫ ＡＳ在蟑螂神经索突触体标本同样也只有单一非协同结合位点,其平均解离常数Ｋｄ与最大结合容量Ｂｍａｘ分别为     

THE VERTICAL BELTS OF NATURAL VEGETATION PARTITIONING OF THE GUANDI MOUNTAINS BY USING ORDERED PLOT CLUSTERING,SHANXI,NORTH CHINA

ＴＨＥＶＥＲＴＩＣＡＬＢＥＬＴＳＯＦＮＡＴＵＲＡＬＶＥＧＥＴＡＴＩＯＮＰＡＲＴＩＴＩＯＮＩＮＧＯＦＴＨＥＧＵＡＮＤＩＭＯＵＮＴＡＩＮＳＢＹＵＳＩＮＧＯＲＤＥＲＥＤＰＬＯＴＣＬＵＳＴＥＲＩＮＧ,ＳＨＡＮＸＩ,ＮＯＲＴＨＣＨＩＮＡ⒇ＦｅｎｇＺｈａｎｇ１Ｔ...     

STUDIES ON THE PATTERN OF MEGASPOROGENESIS AND MICROTUBULAR CYTOSKELETON CHANGES IN CYMBIDIUM SINENSE

ＳＴＵＤＩＥＳＯＮＴＨＥＰＡＴＴＥＲＮＯＦＭＥＧＡＳＰＯＲＯＧＥＮＥＳＩＳＡＮＤＭＩＣＲＯＴＵＢＵＬＡＲＣＹＴＯＳＫＥＬＥＴＯＮＣＨＡＮＧＥＳＩＮＣＹＭＢＩＤＩＵＭＳＩＮＥＮＳＥ￥Ｓ．Ｙ．ＺｅｅＸ．Ｌ．Ｙｅ（１ＢｏｔａｎｙＤｅｐａｒｔｍｅｎｔ,Ｕｎｉ...     

SKF38393抑制大鼠DRG分离神经元GABA-激活电流

在大鼠新鲜分离ＤＲＧ神经元标本上应用全细胞膜片箝记录,观察了多巴胺Ｄ１受体的选择性激动剂ＳＫＦ３８３９３ＨＣＩ对ＧＡＢＡ－激活电流的作用。大部分受检细胞对ＧＡＢＡ敏感,１０＾－６－１０＾－３－ｍｏｌ／Ｌ ＧＡＢＡ可于引起呈剂量依赖性的明显去敏感作用的内向电流。     

HLA—DQ分子遗传结构与中国人重症肌无力的相关性

重症肌无力与ＨＬＡⅡ类基因关联性在不同人种和民族中具有不同遗传易感性,为探讨中国人重症肌无力（ＭＧ）与ＨＬＡ０ＤＱ分子关联性,采用聚合酶链式反应－限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）方法,分析了５０例中国正常人及４９例重症肌无力患者的ＨＬＡ－ＤＱＡ１和－ＤＱＢ１座位的基因型,结果：共检出正常人ＤＱＡ１等位基因８种,ＤＱＢ１等位基因１０种,重症肌无力患者ＤＱＡ１等位基因８种,ＤＱＢ１等位基因９种     

人肝癌细胞的IGF—BP1分子特性及内源性蛋白酶对其降解的研究

用ＳＤＳ－ＰＡＧＥ电泳、高效液相色谱（ＨＰＬＣ）、质谱等方法,研究了人肝癌细胞（ＨｅｐＧ２）分泌的胰岛素样生长因子结合蛋白－１（＾３５Ｓ－ＩＧＦ－ＢＰ１）的分子结构、特性,及其被内源性蛋白酶降解的特点,＾３５Ｓ－ＩＧＦ－ＢＰ１的经抗体免疫沉淀、生化分离,纯化为均一体,其分子是由多个亚构成的蛋白质,分子量约为２７ｋＤ;细胞ＵＭＲ、ＨｅｐＧ２、Ｈ３５ＢＲＬ３Ａ分泌的蛋白酶能将＾３５Ｓ－ＩＧＦ－ＢＰ１催     

畲族Gc、PGM1、AcP_1、6-ΡGD、ADA和AK1的遗传多态性

对畲族血浆组特异性成分（Ｇｃ）、红细胞磷酸葡萄糖变位酶１（ＰＧＭ１）、酸性磷酸酶（ＡｃＰ１）、６－磷酸葡萄糖酸脱氢酶（６－ＰＧＤ）、腺苷脱氨酶（ＡＤＡ）、腺苷酸激酶（ＡＫ１）的遗传多态性进行了研究。Ｇｃ与ＰＧＭ１是用薄层聚丙烯酰胺凝胶等电聚焦分析的,ＡｃＰ１、６－ＰＧＤ、ＡＤＡ及ＡＫ１是用淀粉凝胶电泳分析的。各基因座的基因频率分别为Ｇｃ＊１Ｆ０．４７２２、Ｇｃ＊１Ｓ０．２４２１、Ｇｃ＊２０．２７３８;ＰＧＭ１＊１Ａ０．５３５７、ＰＧＭ１＊１Ｂ０．１６２７、ＰＧＭ１＊２Ａ０．１５８７、ＰＧＭ１＊２Ｂ０．１４２９;ＡｃＰ＊１Ａ０．１８２５、ＡｃＰ１＊Ｂ０．８１７５;６－ＰＧＤ＊Ａ０．９６８３、６－ＰＧＤ＊Ｂ０．０２７８;ＡＤＡ＊１０．９８８１、ＡＤＡ＊２０．０１１９;ＡＫ１＊１１．００００。畲族Ｇｃ、ＰＧＭ１、ＡｃＰ１、６－ＰＧＤ和ＡＤＡ基因为多态,而ＡＫ１基因为单态。发现１例带有６－ＰＧＤ＊Ｒ和３例带有Ｇｃ＊１Ａ２变异等位基因的个体,其中Ｇｃ＊１Ａ２基因频率为０．０１１９,达到多态水平。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.