尖尾枫化学成分及其抗炎活性研究

尖尾枫(Callicarpa longissima)有止血镇痛的功效,其化学成分和药理活性研究报道较少。为研究尖尾枫枝叶的化学成分以及抗炎活性,该实验用尖尾枫枝叶95%乙醇提取物通过柱层析和HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用LPS离体诱导RAW 264.7细胞的NO生成模型,研究主要化合物对NO生成的抑制活性。结果表明:(1)从尖尾枫中分离得到12个化合物,分别鉴定为4'',5-二羟基-7-甲氧基黄酮(1)、喷杜素(2)、蓟黄素(3)、洋艾素(4)、4'',5-二羟基-3'',7-二甲氧基黄酮(5)、甲基条叶蓟素(6)、金腰素(7)、泡桐素(8)、齐墩果酸(9)、桦木酸(10)、2,4,6-三甲氧基苯酚(11)、咖啡酸乙酯(12)。(2)化合物1-7在25 μmol·L^-1浓度下对LPS诱导的小鼠巨噬细胞RAW 264.7释放的NO均具有明显的抑制作用。化合物1、2、4、5、6、8、10、11、12均为首次从该植物中分离得到,化合物1-7均具有不同程度的抗炎作用,其中化合物2、3、6表现出较强的抗炎活性。     

拔毒散的化学成分及其抗炎活性研究

拔毒散(Sida szechuensis)为一种傣族传统的药用植物,常用于治疗诸疮肿毒、皮肤瘙痒、跌打损伤、刀枪伤等疾病。为研究拔毒散的化学成分及其体外抗炎活性,该研究采用硅胶柱色谱、Sephadex LH-20柱色谱、半制备HPLC等方法对其地上部分的化学成分进行分离纯化,通过理化性质和NMR等波谱数据鉴定化合物的结构,并用脂多糖(LPS)诱导的巨噬细胞(RAW 264.7)作为炎症模型,评价所分离化合物的抗炎活性。结果表明:从拔毒散乙醇提取物中共分离得到16个化合物,分别鉴定为kaempferol-3-O-β-D-glucopyranoside(1)、kaempferol-3-O-rutinoside(2)、槲皮素(3)、20-hydroxyecdysone(4)、α-ecdysone(5)、22-deoxyecdysterone(6)、abutasterone(7)、pterosterone(8)、icariside E₅(9)、icariside E₃(10)、(+)-syringaresinol(11)、pinoresinol(12)、balanophonin B(13)、N-trans-feruloyl tyramine(14)、(-)-loliolide(15)、棕榈酸(16),其中化合物1-3、9-13、16首次从该植物中分离得到。抗炎活性测试结果表明,在50μmol·L^-1的浓度下,化合物1、4、5无明显抑制NO生成作用,而化合物2、3、6-16均能在不同程度上抑制NO生成,其中化合物2、3、11-14具有较强的抗炎活性,其IC₅₀值分别为18.63、40.76、21.46、14.32、16.82、42.31μmol·L^-1。该研究结果丰富了拔毒散的化学成分,明确了其具有抗炎效果的物质基础,验证了其传统用途的科学性,为其进一步在医药领域的开发利用提供了新思路及科学依据。     

杏叶防风的化学成分及抗炎活性研究

杏叶防风(Pimpinella candolleana)为贵州苗族习用草药,用于黄疸型肝炎、急性胆囊炎等病症的治疗。为探究杏叶防风的化学成分及其抗炎活性,该研究采用硅胶、凝胶、ODS等色谱技术对杏叶防风全草70%乙醇提取物进行分离纯化,通过NMR、MS等波谱数据鉴定化合物结构,采用脂多糖(LPS)诱导的RAW264.7巨噬细胞作为炎症模型,评价单体化合物的抗炎活性。结果表明:(1)从杏叶防风中分离并鉴定了20个化合物,分别为香草醛(1)、芝麻素(2)、2-甲基-2-羟基-5-甲氧基苯并 [d] 氢化呋喃-3-酮(3)、原儿茶醛(4)、1,5-dihydroxy-2,3-dimethoxyxanthone(5)、异鼠李素(6)、山奈酚(7)、8-羟基-2-甲基色原酮(8)、木犀草素(9)、槲皮素(10)、1-O-β-D-葡萄糖-(2S,3S,4R,8E)-2- [(2''R)-2''-羟基棕榈酰胺]-8-十八烯-1,3,4-三醇(11)、异鼠李素-3-O-β-D-半乳糖苷(12)、异槲皮苷(13)、去甲当药醇苷(14)、木犀草素-6-C-α-L-阿拉伯糖苷(15)、山奈酚-3-O-β-D-半乳糖苷(16)、山奈酚-7-O-β-D-葡萄糖苷(17)、木犀草素-7-O-β-D-葡萄糖苷(18)、异牡荆苷(19)、芦丁(20)。其中,化合物1、3、4、6、7、10、13、16、18、20均为首次从该植物中分离得到。(2)抗炎结果显示,化合物2-10、12、18、19均可显著抑制 LPS 诱导 RAW264.7 细胞NO释放量(P<0.05,P<0.01),其中化合物4、7、10、18在浓度为25 μmol·L^-1时,抑制率分别为57.37%、83.60%、68.16%、81.14%。该研究丰富了杏叶防风的化学成分,明确了黄酮类化合物是其发挥抗炎功效的活性成分,为杏叶防风的进一步研究与开发利用提供了一定的依据。     

Chemical constituents of the roots of Scorzonera divaricata and their chemotaxonomic significance

The phytochemical study of the roots of Scorzonera divaricata Turcz led to the isolation of 27 compounds, including eight sterols (1–8), one lignan (9), two cumarins (10, 11), five phenylpropanoids (12–16), six benzene derivatives (17–22), methyl-β-D-fructofuranoside (23), monolinolein (24), and three aliphatic acids (25–27). The structures of isolated compounds were identified using NMR and ESI-MS spectroscopic methods and comparing them with those previously reported. Except for β-daucosterol (8), scopoletin (10) and caffeic acid (16) from S. divaricata, this is the first report of the other 24 compounds from S. divaricata. Among them, eleven compounds (2–6, 11, 17, 19, 20, 23, 25) were reported from genus Scorzonera for first time, suggesting that they could be used to distinguish S. divaricata from the other species of Scorzonera. Furthermore, the chemotaxonomic significance of isolated compounds from S. divaricata has also been discussed.     

蛇含委陵菜的化学成分及抗炎活性研究

为了研究蛇含委陵菜(Potentilla kleiniana)的化学成分及其抗炎活性,该文利用D-101大孔树脂、硅胶及Toyopearl HW-40F等色谱技术对蛇含委陵菜60%乙醇提取物进行分离纯化,通过NMR和HR-ESI-MS波谱数据鉴定化合物的结构,采用小鼠巨噬细胞(RAW 264.7)体外炎症模型评价化合物的抗炎活性。结果表明:(1)从蛇含委陵菜中分离得到15个化合物,分别鉴定为2-(heptadecanoyloxy)propane-1,3-diyl distearate(1)、9,12,13-三羟基-10,15-十八碳二烯酸(2)、9,12,13-三羟基-10,15-十八碳二烯酸甲酯(3)、2,2''-氧代双(1,4-二叔丁苯)(4)、大黄素(5)、大黄酚(6)、(6R,9R)-3-酮-α-紫罗兰醇-9-O-β-D-吡喃葡萄糖苷(7)、新穿心莲内酯(8)、甲基-α-D-呋喃果糖苷(9)、1-O-β-D-吡喃果糖-α-D-吡喃阿洛糖苷(10)、对香豆酸(11)、cesternosides A(12)、koaburaside(13)、荭草素(14)、异荭草素(15),均首次从委陵菜属植物中分离得到。(2)抗炎实验结果显示,化合物1-3、8、11-15具有一定NO释放抑制活性,其中化合物8在 25 μmol·L^-1浓度下抑制率为72.5%。该研究丰富了蛇含委陵菜的植物化学信息,明确了脂肪酸衍生物、酚性成分及二萜类成分是其抗炎活性成分,为蛇含委陵菜的进一步开发利用提供了理论依据。     

Steroidal glycosides from the roots of Cynanchum stauntonii and their effects on the expression of iNOS and COX-2

Six new steroidal glycosides, named stauntosides O-T (1–6), along with eight known compounds (7–14), were obtained from the 95% aqueous ethanol extract of the roots of Cynanchum stauntonii. Their chemical structures were elucidated by IR, HR ESI-MS/MS, ¹H- and ¹³C NMR, ¹H-¹H COSY, HSQC, HSQC-TOCSY, and HMBC spectroscopic analyses, which showed interesting 13,14:14,15-disecopregnane-type or 14,15-secopregnane-type C₂₁ steroidal glycosides. The glycosides’ anti-inflammatory effects were investigated by detecting the inhibitory effects of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) on RAW246.7 murine macrophage cells stimulated by lipopolysaccharide (LPS). Our results showed that compounds 1, 5, 8, 9, 11, and 13 could significantly inhibit iNOS expression, and compounds 5 and 7 could clearly reduce COX-2 expression in RAW246.7 cells stimulated by LPS compared to cells stimulated with LPS and not treated with other compounds. Thus, compounds 1, 5, 7–9, 11, and 13 have the potential to mediate anti-inflammatory effects, with compound 5 having a greater anti-inflammatory effect than the other compounds.     

非洲白参的抗氧化活性及其化学成分研究

采用DPPH（1,1-二苯基-2-三硝基苯肼）、ABTS（2,2-联氮-双-3-乙基苯并噻唑-6-磺酸）自由基清除法和FRAP（铁离子还原/抗氧化能力）法对非洲白参（Mondia whiteii（Hook.f.）Skeels）不同萃取部位的抗氧化活性进行分析,然后采用紫外分光光度法和福林酚比色法分别测定非洲白参不同萃取部位的总黄酮、总酚含量,最后采用大孔树脂AB-8、ODS以及Sephadex LH-20柱层析和半制备型HPLC等色谱分离技术对其二氯甲烷萃取部位的化学成分进行分离、纯化。结果显示：非洲白参二氯甲烷萃取部位抗氧化活性最高,且二氯甲烷萃取部位的总酚含量远高于其他部位;化学成分分离、纯化后得到10个单体化合物,分别为：2-羟基-4-甲氧基苯甲醛（1）、秦皮素（2）、7-甲氧基香豆素（3）、α-羟基丁香丙酮（4）、ω-hydroxypropioguaiacone（5）、桂皮酸（6）、水杨酸（7）、4-甲氧基水杨酸（8）、丁香酸（9）、壬二酸（10）。其中,化合物3~10为首次从该植物中分离得到。     

红凉伞根化学成分及抗炎活性研究

红凉伞(Ardisia crenata var.bicolor)为贵州苗族常用药,具有清喉利咽、消肿止痛、祛风除湿等功效。为研究红凉伞根化学成分及体外抗炎活性,该文采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、ODS反相柱色谱及半制备HPLC等方法对红凉伞根70%乙醇提取物进行系统性研究,结合NMR、MS等现代波谱技术分析及文献对比进行化合物结构鉴定。采用脂多糖(LPS)诱导的小鼠RAW 264.7巨噬细胞的NO生成模型,评价化合物的抗炎活性。结果表明：(1)从红凉伞根70%乙醇提取物中分离得到10个化合物,分别鉴定为11-O-没食子酰岩白菜素(1)、11-O-(4-O-甲基没食子酰)岩白菜素(2)、11-O-香草酰岩白菜素(3)、6-O-(4-羟基苯甲酰基)岩白菜素(4)、11-丁香酰岩白菜素(5)、11-O-(3′,4′-二甲基没食子酸)-岩白菜素(6)、去甲氧基岩白菜素(7)、micractinin A(8)、monomethyl olivetol(9)、邻苯二甲酸二丁酯(10),其中化合物4、8、9为首次从紫金牛属中分离得到。(2)体外抗炎活性实验结果表明,化合物1-...     

北豆根化学成分及其抗炎活性研究

北豆根,防己科蝙蝠葛(Menispermum dauricum)的干燥根茎,是重要的中药材。为阐明北豆根的药效物质基础,该研究利用硅胶柱层析、大孔吸附树脂及高压制备HPLC等色谱技术对北豆根化学成分进行系统分离,采用NMR等波谱技术对化合物进行结构鉴定,并通过测定化合物对脂多糖(LPS)诱导的巨噬细胞RAW264.7释放NO和IL-6炎症因子的含量评价其抗炎活性。结果表明：(1)从北豆根甲醇提取物中分离鉴定了15个化合物,分别鉴定为对羟基苯甲醛(1)、香草酸(2)、丁香醛(3)、2-羟基-1-(4-羟基-3,5-二甲氧基苯基)-1-丙酮(4)、对羟基苯乙酸甲酯(5)、2-(4-羟苯基)-硝基乙烷(6)、对羟基苯乙腈(7)、邻苯二甲酸二丁酯(8)、fragransin B₂(9)、7-hydroxy-3,6-dimethoxy-1,4-phenanthraquinone(10)、棕榈酸(11)、花生酸(12)、β-谷甾醇(13)、β-豆甾醇(14)、胡萝卜苷(15)。其中,化合物4-7、9、12为首次从防己科植物中分离得到,化合物1、3-11、14为首次从蝙蝠葛属植...     

Chemical constituents of Aeschynanthus bracteatus and their weak anti-inflammatory activities

Chemical examination of the EtOAc extract from the aerial parts of Aeschynanthus bracteatus led to isolation of four phenylethanol glycosides, aeschynanthosides A-D (1-4), and 55 known constituents, including 8 phenylethanoids, 23 phenols, 5 lignans, 7 flavonoids, 9 terpenoids, and 4 others. Their structures were elucidated mainly by detailed spectroscopic studies and comparison with published data. All 59 compounds were isolated for the first time from an Aeschynanthus species. The isolates were also tested for inhibitory activities against LPS-induced NO production in RAW 264.7 macrophages. Aeschynanthoside D (4) and naringenin (41), within the concentration arrange tested (50-100 microg/mL), showed very weak dose-dependent effects with the inhibition rate of 24.2%, 35.4%, 66.0%, and 9.5%, 40.1%, 65.0%, respectively, relative to positive controls.     

民族药刺梨根茎化学成分及其抗炎活性研究

民族药刺梨根茎在贵州少数民族地区有着广泛的应用,为验证其化学成分的抗炎活性,该文以民族药新鲜刺梨根茎为原料,采用硅胶柱色谱、Sephadex LH-20柱色谱等方法对其根茎的化学成分进行分离纯化,通过理化性质和NMR等波谱数据鉴定化合物的结构; 采用脂多糖(LPS)诱导的小鼠巨噬细胞RAW 264.7作为炎症模型,考察刺梨根茎化学成分对巨噬细胞经LPS刺激后产生的NO炎症因子的影响,评价其抗炎活性。结果表明:(1)从刺梨根茎乙醇提取物中共分离获得15个化合物,结构分别鉴定为刺梨苷(1)、野蔷薇苷(2)、蔷薇酸(3)、β-D-glucopyranosyl-(2a→1b)-2a-O-β-L-arabinopyranosyl-(2b→1c)-2b-O-β-L-arabinopyranosyl-(2c→1d)-2c-O-β-L-arabinopyranosyl-(2d→1e)-2d-O-β-L-arabinopyranosyl-(2e→1f)-2e-O-β-L-arabinopyranoside(4)、儿茶素(5)、3-O-methylellagic acid-4''-O-β-D-xylopyranoside(6)、3-O-methylellagic acid-4''-O-α-L-rhamnopyranoside(7)、委陵菜酸(8)、桦木酸(9)、spinosic acid(10)、arjunic acid(11)、 β-谷甾醇(12)、β-胡萝卜苷(13)、α-tocopherol(14)、正二十六烷(15)。其中化合物4、6、7首次从该植物中分离得到。(2)对其中的化合物1-7进行体外抗炎活性实验,结果发现化合物1-7对LPS诱导的小鼠巨噬细胞RAW 264.7释放的NO均有明显抑制作用,且呈剂量依赖关系; 化合物1-7在抗炎作用上表现出较好活性,其IC₅₀值分别为25.07、24.56、17.65、9.87、16.67、40.83、34.98 μmol·L^-1(阳性对照地塞米松为22.46 μmol·L^-1 ),其中化合物3、4、5的活性优于地塞米松。该研究结果阐明了刺梨根茎中的三萜类、鞣花酸类、黄酮类和寡糖类化合物是其抗炎作用的主要有效成分,并验证了刺梨根茎的民间抗炎功效。     

广东土牛膝的化学成分

广东土牛膝为菊科泽兰属植物华泽兰（Eupatorium chinense）的干燥根。从其甲醇提取物中共分离得到11个化合物,其中eupatorinA（1）为一新化合物,经波谱学方法鉴定为（threo）-3-O-acetyl-1-（4-hydroxy-3-methoxyphenyl）-2-[4-（3-hydroxy-1-（E）-propenyl）-2,6-dimethoxyphenoxy]propyl-β-D-glucopy-ranoside。已知化合物分别鉴定为（threo）-3-hydroxy-1-（4-hydroxy-3-methoxyphenyl）-2-[4-（3-hydroxy-1-（E）-propenyl）-2,6-dimethoxyphenox-y]-propyl-β-D-glucopyranoside（2）,ardisiacrispinA（3）,ardisiac-rispinB（4）,euparone（5）,3-（2,3-dihydroxy-isopen-tyl）-4-hydroxyacetophenone（6）,12,13-di-hydroxy-euparin（7）,gymnastone（8）,N-（2′-hydroxy-tetracosanosyl）-2-amino-1,3,4-trihydroxy-octa-dec-8-（E）-ene（9）,stigmasterol（10）和stigmasterol-3-O-β-D-glucopyranoside（11）。化合物2-4为首次从菊科植物,5-8为首次从泽兰属植物中分离得到。     

Chemical constituents from the roots of Pteroxygonum giraldii Damme et Diels

Twenty-four compounds were obtained from Pteroxygonum giraldii and were classified as five triterpenes (1–5), two sterols (6 and 7), two quinones (8 and 11), three stilbenes (9, 10 and 18) and twelve flavonoids (12–17, 19–24). Seventeen of these compounds were obtained from the genus Pteroxygonum for the first time. The chemotaxonomic significance of this plant was also discussed. Compounds 10, 12 and 23 can serve as the fingerprints for P. giraldii, and compounds 12, 13, 17 and 22–24 are recognized as typical metabolites of P. giraldii. Both Pteroxygonum and Polygonum might have the same origin and could be classified into the same tribe among Polygonaceae. Furthermore, due to the presence of stilbenes, Pteroxygonum may have some taxonomic relationships with Fallopia, Rumex and Reynoutria.     

Compounds from the fruits of Nicandra physaloides and their potential anti-inflammatory activities

Twenty-one compounds were isolated and identified from the ethanol extract of the fruits of Nicandra physaloides, including four new (1–4) and 17 known compounds (5–21). All the compounds were isolated from the genus Physalis for the first time. In this study, NMR spectroscopy was used to study the compound structures by comparing their data with those reported in the literature. Moreover, the potential anti-inflammatory activity of RAW264.7 cells induced by lipopolysaccharide (LPS) was evaluated. Among the monoterpenoids, compounds (5–7, 9) exhibited weak anti-inflammatory activities, with IC₅₀ values ranging from 29.10 to 68.30 μM.     

Chemical constituents from Alismatis Rhizoma and their anti-inflammatory activities in vitro and in vivo

Six new compounds, including a new compound with an unusual 2, 4, 6-cycloheptatrien ketone skeleton (1), two new diphenylpropanoid ethers (2, 3), a new protostane-type triterpenoid (4), two new norsesquiterpene (5a, 5b), and two new natural products (6, 7), together with eleven known compounds (8–18) were isolated from the aqueous extract of Alismatis Rhizoma (AR). Their structures were elucidated by a combination of 1D and 2D NMR (¹H and ¹³C NMR, COSY, HSQC, HMBC, and NOESY), HRESIMS spectroscopic data, experimental and calculated electronic circular dichroism (ECD) spectra. Some of the compounds were evaluated for their inhibitory effects on nitric oxide (NO) production in LPS-induced RAW 264.7 cells. Two protostane-type triterpenoids, compounds 4 and 17, exhibited potent inhibitory activities with the IC₅₀ values of 39.3 and 63.9 μM compared with indomethacin. In the meanwhile, their anti-inflammatory effects were also confirmed by acute inflammation model induced by CuSO₄ in zebrafish.     

Chemical constituents from the fresh flower buds of Musa nana and their chemotaxonomic significance

Phytochemical study on the fresh flower of Musa nana Lour. provided seventeen known compounds including two alkaloids, 3-(hydroxyacetyl)-indole (1), bi-indol-3-yl (2), two terpenoids, 5-[(1R)-1-hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexen-1-yl]-3-methyl-, (2Z, 4E) −2, 4-pentadienoic acid (Valdes), 5, 6(S), 7, 7a(R)-tetrahydro-6-hydroxy-4,4-dimethyl-2(4H)-benzofuranone (4), seven phenols (5–11), three phenylphenalenones, 2-hydroxy-4-(4-methoxyphenyl)-1H-phenalen-1-one (12), 2-methoxy-9-phenyl-1H-phenalen-1-one (13), 2-methoxy-9-(4-methoxyphenyl)-1H-phenalen-1-one (14), and three lipids (15–17). In the present study, all the compounds were isolated for the first time from the species M. nana. Ten compounds including 1-8 and 15-16 have never been previously encountered in the Musaceae family. Furthermore, the chemotaxonomic significance of these isolates was also discussed.     

Chemical constituents from the whole herbs of Peperomia tetraphylla and their chemotaxonomic significance

Our studies report the isolation of seventeen compounds (1–17), which include three phenolic acids (12–14), seven flavonoids (3, 6, 8, 11, 15–17), four secolignans (1, 2, 5 and 9), one tetrahydrofuran lignin (10) and two alkaloids (4 and 7). These compounds have been obtained from the Peperomia tetraphylla using chromatographic methods. Their structures were confirmed based on spectroscopic methods. Among them, five compounds (4, 7, 8, 11 and 15) were found in the family, four compounds (3 and 12–14) were firstly reported from genus Peperomia, and four compounds (6, 9, 16 and 17) were firstly obtained from this species. Furthermore, the chemotaxonomic significance of these ingredients from the whole plants of P. tetraphylla has been discussed in detail.     

Chemical constituents from Vietnamese mangrove Calophyllum inophyllum and their anti-inflammatory effects

In a search for anti-inflammatory activity in resources from Vietnamese mangroves, we found that a methanolic extract from the leaves of Calophyllum inophyllum (CIL) showed significant anti-inflammatory effects in vitro. Using various chromatographic techniques, we subsequently isolated 12 compounds (1–12) from a methanolic extract of CIL, including two novel compounds (1–2). The inhibitory effects of these compounds on lipopolysaccharide-induced nitric oxide (NO) production in RAW264.7 cells were also evaluated. Compound 1 significantly suppressed NO production (IC₅₀ = 2.44 ± 0.88 µM), the secretion of pro-inflammatory cytokines (including interleukin-1 beta and tumor necrosis factor alpha), and the expression of inducible nitric oxide synthase through downregulation of nuclear factor-kappa-B signaling cascades. These results suggest that C. inophyllum leaves might be a useful resource for the development of drugs for the treatment of inflammation.     

木棉根化学成分研究

为了解木棉(Bombax ceiba)的化学成分,从其根中分离鉴定了17个化合物,经理化性质和波谱分析,分别鉴定为24-烯-环阿尔廷酮(1)、环阿尔廷醇(2)、木栓酮(3)、羽扇豆醇(4)、3β-乙酰齐墩果酸(5)、齐墩果酸(6)、麦角甾-4,6,8(14),22E-四烯-3-酮(7)、胆甾-4-烯-3-酮(8)、5α,8α-环二氧-24-甲基-胆甾-6,9,22E-三烯-3β-醇(9)、β-谷甾醇(10)、6β-羟基-豆甾-4-烯-3-酮(11)、胆甾醇(12)、β-谷甾醇-3-O-β-D-葡萄糖苷-6′-O-二十烷酸酯(13)、亚油酸三甘油酯(14)、正三十一烷醇(15)、正二十二烷酸(16)、油酸月桂醇酯(17)。除化合物4、6、9、10外,其余化合物均为首次从该属植物中分离得到。     

Chemical constituents from the pericarps of Zanthoxylum bungeanum and their chemotaxonomic significance

The phytochemical study of the pericarps of Zanthoxylum bungeanum Maxim under the guidance of bioactivity led to the isolation of 18 compounds, including a new isobutylhydroxyamide (1) and 17 known compounds, i.e. six alkylamides (2–7), five coumarins (8–12), one benzene derivative (13), three flavonoids (14–16), and two sterols (17–18). Their structures were elucidated based on extensive spectroscopic methods (HRESIMS, 1D and 2D NMR experiments) and by comparison with literature data. New compound (1) and known compound (2) are cis-trans isomeric isobutylhydroxyamides. Among them, compounds 9, 10, and 12 were isolated for the first time from Z. bungeanum, compound 11 was firstly recovered from the genus Zanthoxylum, and compound 14 was reported for the first time from the Rutaceae family. The chemotaxonomic significance of isolated compounds from Z. bungeanum is discussed.