Two new species ofMycena from eastern Honshu, Japan

Two new species ofMycena are described and illustrated from eastern Honshu, Japan:Mycena brevicapillata sp. nov. (sectionHiemales), forming tall and slender basidiomata covered overall with long, fusiform or sublageniform dermatocysts, was found on a dead branch ofHydrangea involucrata; Mycena chrysanthemiformis sp. nov. (sectionFragilipedes), forming small, white basidiomata with a campanulate, shallowly sulcate-striate, occasionally subumbonate pileus and adnate-decurrent lamellae, was found on living bark or a dead fallen twig ofAphananthe aspera, Cryptomeria japonica, andZelkova serrata.     

Two new species ofMarasmiellus from eastern Honshu, Japan

Two new species ofMarasmiellus are described from eastern Honshu, Japan:Marasmiellus atrostipitatus sp. nov. (sectionRameales subsectionOpacini) is characterized by a white pileus and a blackish stipe covered with white pruinose to flocculos scales.Marasmiellus brunneocarpus sp. nov. (sectionTricolores) has small brownish basidiomata. Both species occur on dead leaves and twigs inQuercus-Eurya forests.     

Two new species ofMarasmius from eastern Honshu, Japan

Two new species belonging toMarasmius (Agaricales) are described and illustrated from eastern Honshu, Japan:Marasmius capitatus sp. nov. (sectionEpiphylli), forming capitate cystidia and small basidiomata with a membranous, white pileus and a minutely pubescent, filiform stipe without basal mycelium, was found on dead fallen leaves ofCryptomeria japonica; Marasmius nivicola sp. nov. (sectionGlobulares), having entirely white, collybioid basidiomata with a furfuraceous to pruinose stipe and distinctly intervenose lamellae, was found on leaf litter of broad-leaved forest.     

Three new species ofMarasmius sectionSicci from eastern Honshu, Japan

Three new species belonging toMarasmius sectionSicci (Agaricales) are described and illustrated from eastern Honshu, Japan:Marasmius nocturnus sp. nov., forming a dark brown pileus and marginate lamellae, was found on leaf litter inPasania—Quercus forests;Marasmius occultatus sp. nov., producing brownish orange or light brown basidiomata and long, cylindrical-fusoid basidiospores, was found on dead fallen twigs ofAphananthe aspera andQuercus myrsinaefolia;Marasmius opulentus sp. nov., having a reddish orange pileus and a pubescent stipe, was found on leaf litter in laurel-leaved forest.     

A new quadrannulate species of Orobdella (Hirudinida,Arhynchobdellida, Orobdellidae) from western Honshu,Japan

A new quadrannulate species of Orobdella Oka, 1895, Orobdella naraharaetmagarum sp. n., from the mountainous region of western Honshu, Japan is described. Orobdella naraharaetmagarum is a small species with a body length of less than 5 cm. Phylogenetic analyses using nuclear 18S rRNA and histone H3, as well as mitochondrial cytochrome c oxidase subunit I, tRNA^Cys, tRNA^Met, 12S rRNA, tRNA^Val, 16S rRNA, tRNA^Leu and NADH dehydrogenase subunit 1 markers indicated that the present new species is the sister species of the quadrannulate Orobdella esulcata Nakano, 2010. Furthermore, mitochondrial DNA genealogy within Orobdella naraharaetmagarum demonstrated that this new species is divided into eastern and western lineages.     

A new quadrannulate species of Orobdella (Hirudinida,Arhynchobdellida, Orobdellidae) from central Honshu,Japan

A new quadrannulate species of Orobdella, Orobdella masaakikuroiwai sp. n., from the mountainous region of central Honshu, Japan is described. This is only the second small species known within this genus, with a body length of less than 4 cm for mature individuals. Phylogenetic analyses using nuclear 18S rDNA and histone H3 as well as mitochondrial COI, tRNA^Cys, tRNA^Met, 12S, tRNA^Val, 16S, and ND1 markers showed that Orobdella masaakikuroiwai sp. n. is the sister species of the quadrannulate Orobdella whitmani Oka, 1895. Phylogenetic relationships within Orobdella masaakikuroiwai sp. n. conducted using mitochondrial markers reveled a distinction between eastern and western phylogroups.     

Trimma albicaudatum,a new species of pygmygoby from Sagami Bay,Honshu, Japan (Teleostei: Gobiidae: Gobiinae)

Ichthyological Research - The pygmygoby Trimma albicaudatum sp. nov. is described on the basis of a single specimen (18.0 mm standard length) from Sagami Bay, Honshu, Japan. The new species can be...     

A new species of salamander of the genus Hynobius from Central Honshu, Japan (Amphibia, Urodela)

We describe a small salamander from south Central Honshu, Japan, as a new species, Hynobius katoi. The genetic distances between this species and several named species, including sympatric H. kimurae, derived from allozyme data from a starch gel electrophoresis, proved to be sufficiently large to differentiate it at a specific rank. Distribution of this species is confined to the montane regions of Shizuoka and Nagano Prefectures, on the Akaishi Mountains of the Chubu District, central Japan. It is regarded as a member of the naevius group of Hynobius, characterized by small number of large, pigmentless ova. The species differs from the other species of the naevius group by the combination of relatively small body size, nearly spotless body, relatively few vomerine teeth forming moderately shallow series, and unique electrophoretic pattern of isozymes.     

A revision of the family Leucochloridiidae Poche (Digenea) and studies on the morphology of Leucochloridium paradoxum Carus, 1835

Summary The sporocyst and metacercaria of Leucochloridium paradoxum Carus, recovered from Succinea putris L. in Norway, are described and figured. Adults recovered from experimental infection of Taeniopygia guttata (Vieillot) with L. paradoxum metacercariae are described, using results from scanning electron microscopy, and compared with earlier information. The family Leucochloridiidae Poche is revised. Four genera are recognized within the family (i.e. Leucochloridium Carus, 1835; Urogonimus Monticelli, 1888; Urotocus Looss, 1899; Michajlovia Pojmanska, 1973). The larval ecological and morphological characteristics are tabulated for the three genera with known larval stages. The status of the genus Neoleucochloridium Kagan, 1951, is changed, on the grounds of larval similarities with Leucochloridium Carus. This means that within the genus Leucochloridium Carus three subgenera are erected, based primarily on sporocyst characteristics. These are Leucochloridium, Neoleucochloridium and Papilloleucochloridium. The diagnostic larval and adult characters are tabulated. The known larval records of species in the genus Leucochloridium are discussed and revised within the framework of this new classification. ac]19790812     

Marasmius brunneospermus, a new species ofMarasmius sectionGlobulares from central Honshu, Japan

A new species ofMarasmius sectionGlobulares, Marasmius brunneospermus sp. nov., found in the lowland forest of Kanagawa and Chiba, Japan, is described and illustrated. This species is characterized by a hygrophanous pileus with rugulose-reticulate disk, well-developed basal mycelium attached to an extensive mycelial mat in leaf litter, and conspicuous fusoid-ventricose pleurocystidia. In addition, its brown spore print mottled with white parts is unusual as an infrageneric character in the genusMarasmius.     

Food plants and life cycle of Lymantria bantaizana Matsumura (Lepidoptera: Lymantriidae) in northern Honshu, Japan

The food plants and life cycle of Lymantria bantaizana were investigated in Iwate Prefecture, northern Honshu, Japan from 2000 to 2002. Eggs laid in July hatched approximately 10 days after oviposition. Hatched larvae fed only on Juglandaceae, Juglans mandshurica var. sachaliensis, J. regia var. orientalis and Pterocarya rhoifolia when reared in the laboratory. In field rearing, the plants of Juglans enabled the moth to complete its life cycle. Differing from all other known Lymantria species in Japan, the moth overwintered not in the egg stage but in the fifth or sixth instar larval stage. Lymantria bantaizana had eight larval instars in both sexes. Moth emergence occurred mostly in July at a field‐trapping site in Iwate Prefecture.     

Phylogeography of Rhabdias spp. (Nematoda: Rhabdiasidae) collected from Bufo species in Honshu,Shikoku, and Kyushu,Japan including possible cryptic species

The genus Rhabdias Stiles & Hassall, 1905 comprises lung parasites of amphibians and reptiles worldwide. In Japan, 9 species have been recorded, including Rhabdias incerta Wilkie, 1930 which has been reported only in Bufo species. In this study, to assess the diversity of R. incerta, we performed molecular analyses of Rhabdias species sampled from three species/subspecies of Japanese toads namely Bufo japonicus, B. japonicus formosus, and B. torrenticola, collected in various regions of Honshu, Shikoku, and Kyushu, Japan. DNA sequence divergence was compared using mtDNA (COI) and nuclear DNA (28S) to identify possible cryptic species. Morphological analysis was performed through light microscopy and scanning electron microscopy (SEM). The results revealed that Bufo spp. serve as hosts for at least three Rhabdias species. Morphologically, most samples were identified as R. incerta but with a longer body and larger buccal cavity than originally described. Rhabdias incerta appears to be specific to the genus Bufo and is further subdivided into two or three phylogroups based on subspecies divisions and biogeography of their host. Some Rhabdias specimens collected in this study resemble R. tokyoensis Wilkie, 1930, parasitic in hosts from the order Caudata, which suggests host switching. Both molecular and morphological analyses suggested the presence of undescribed and cryptic Rhabdias species within toads collected in Japan. This study was the first to molecularly characterize Rhabdias species in Japan, including novel sequences of R. incerta and two undescribed species.     

Two new species and one new variety of Agaricales from central Honshu, Japan

Two new species and one new variety of Agaricales are described and illustrated from central Honshu, Japan:Clitocybe trogioides var.odorifera var. nov. (subgenusCystoclitus sectionCystoclitus), forming white, infundibuliform basidiomata, was collected from leaf litter in theQuercus-Pasania forests;Gerronema nemorale sp. nov. (sectionXanthophylla), forming small, olivaceous, omphalinoid basidiomata, was found on dead fallen twigs in theQuercus-Pasania forests;Psathyrella cineraria sp. nov. (subgenusMycophylla sectionArgillosporae), forming basidiomata covered by detersile, dark grey, fibrillose-squamulose veil, was found on decayed wood ofQuercus myrsinaefolia.     

Life cycle of Genarchopsis goppo (Trematoda: Derogenidae) from Nara, Japan.

A cystophorous cercaria obtained from Semisulcospira libertina (Gould) from Tobihino, Nara, Honshu, Japan, was used to experimentally infect a freshwater goby Rhinogobius sp. OR via the copepods Mesocyclops leuckarti (Claus), Thermocyclops hyalinus (Rehberg), and Eucyclops sarrulatus (Fisher). It was shown to grow into Genarchopsis goppo Ozaki, 1925. The parasite was found to occur as a natural infection in the stomach of Rhinogobius sp. OR and Odontobutis obscura (Temminck and Schlegel) from Tobihino.