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1.
Potato root cultures transformed bypRiA4 T-DNA do not regenerate plants in vitro spontaneously, but regeneration can be induced by a sequence of callogenesis
and regeneration media. Both Tl and Tr-DNA were found by Southern blotting. Regenerated transformants showed characteristic morphological deviations in the invitro cultures. Plants in the soil showed differences in the inflorescence and tuber development. 相似文献
2.
Robert Van Der Heijden Robert Verpoorte Hens J. G. Ten Hoopen 《Plant Cell, Tissue and Organ Culture》1989,18(3):231-280
The literature concerning the formation of secondary metabolites in cell and tissue cultures ofCatharanthus roseus has been reviewed. Several aspects involved in the formation of secondary metabolites are discussed; e.g. regulation of secondary metabolism, environmental factors influencing secondary metabolism, biosynthesis and enzymology of the products, analysis of product formation, immobilization of cultured cells and stability of cell lines. Some economical aspects of production processes are discussed. 相似文献
3.
Summary TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin vitro. Adventitious buds were fonned and shoots were regenerated on kanamycin selection medium 3-4 months after inoculation. Integration of the NPTII and the GUS genes was confirmed by polymerase chain reaction (PCR) and by Southern hybridization analyses. Histochemical GUS assay showed that the inserted gene was expressed in all tissues with the cauliflower mosaic virus (CaMV) 35S promoter. This transformation procedure has the potential to expand the range of genetic variation inRhododendron. 相似文献
4.
L. Semeria B. Ruffoni M. Rabaglio A. Genga A. M. Vaira G. P. Accotto A. Allavena 《Plant Cell, Tissue and Organ Culture》1996,45(1):67-72
Callus cultures were initiated from micropropagated Artemisia absinthium plantlets on MS basal medium supplemented with different concentrations of BA, Kn, NAA, IAA and 2,4-d in combination or singly. Supplementing the medium with low doses of both BA in combination with NAA, and Kn in combination with NAA enhanced the growth rate of callus cultures. However, cultures grew slowly following the second subculture and the majority turned brown and died within the next month. Initiation of root and shoot primordia occured directly from leaf explants cultured on 1.81 M 2,4-d, while adventitious shoot formation from callus was observed occasionally when BA was added to the medium in combination with IAA. Furthermore, medium containing 2.22 M BA and 2.69 M NAA stimulated both callus growth and organogenesis on some callus cultures derived from leaves and stems of young stock material. The best results were obtained with leaf explants. Cytological analysis of root meristems revealed that all regenerants were diploid (2n=18), as expected.Abbreviations MS
Murashige & Skoog's salts and vitamins (1962)
- BA
6-benzyladenine
- NAA
alphanaphthaleneacetic acid
- Kn
Kinetin (6-furfurylaminopurine)
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IAA
indoleacetic acid
- FW
fresh weight
- Bi
biomass increase 相似文献
5.
Genetic transformation using Agrobacterium rhizogenes 总被引:1,自引:0,他引:1
D. Tepfer 《Physiologia plantarum》1990,79(1):140-146
UDP-glucose pyrophosphorylase (EC 2.7.7.9) has been highly purified from the plant fraction of soybean ( Glycine max L. Merr. cv Williams) nodules. The purified enzyme gave a single polypeptide band following sodium docecyl sulphate polyacryla-mide gel electrophoresis, but was resolved into three bands of activity in non-denaturing gels. The enzyme appeared to be a monomer of molecular weight between 30 and 40 kDa. UDP-glucose pyrophosphorylase had optimum activity at pH 8.5 and displayed typical hyperbolic kinetics. The enzyme had a requirement for divalent metal ions, and was highly specific for the substrates pyrophosphate and UDP-glucose in the pyrophosphorolysis direction, and glucose-1-phosphate and UTP in the direction of UDP-glucose synthesis. The Km values were 0.19 m M and 0.07 m M for pyrophosphate and UDP-glucose, respectively, and 0.23 m M and 0.11 m M for glucose-1-phosphate and UTP. The maximum velocity in the pyrophosphorolysis direction was almost double that for the reverse reaction. UDP-glucose pyrophosphorylase did not appear to be subject to a high degree of fine control, and activity in vivo may be regulated mainly by the availability of the substrates. 相似文献
6.
Analysis of 76 cell clones derived from one leaf of a periwinkle plant (Catharanthus roseus (L.) G. Don) showed the occurrence of Corynanthe-, Strychnos-, and Aspidosperma-type alkaloids. The majority of clones (62%) displayed compounds of all three types. Variation of the alkaloid spectra of the cell clones was low when compared to that found previously with serially subcultured callus and cell suspensions derived from different plants.NRCC # 19100 相似文献
7.
Anin vitro transformation method has been developed for stem explants of fast-growing willow clones (Salix spp.) usingAgrobacterium tumefaciens as a vector. Transformants obtained with the strains C58 and GV3101 (pGV3851::pLD1) were selected on hormone-free medium and on medium containing kanamycin, respectively. Transformation was confirmed by Southern blot analysis and nopaline assay. Inoculation of green-house grown plants with nopaline and octopine wildtype strains and shoot or root inducing mutant strains caused undifferentiated tumors at a frequency of 0 to 80%, depending on theSalix genotype and the bacterial strain used.Abbreviations BA
benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- Km
kanamycin
- NPT
neomycin phosphotransferase 相似文献
8.
Petunia hybrida plants were inoculated with differentAgrobacterium rhizogenes andA. tumefaciens strains and developed tumors were further cultivatedin vitro. Transformed flowering plants differentiated from tumors induced byA. rhizogenes strains 8196 and TRIOL Transformed but non-rooted plants developed also from tumors incited byA. tumefaciens T37. Cultures of roots transformed byA. rhizogenes strain 15834 did not show increased incidence of chromosomal aberrations in anaphases in comparison with untransformecl control.
Permanent growth of isolated untransformedPetunia roots was not induced by addition of IAA into the medium. 相似文献
9.
Summary Adsorption ofC.
roseus cells producing indole alkaloids on various support particles were investigated in an attempt to find a suitable support material for surface culture of plant cells. Five different support particles namely gelatin, agar, alginate, polypropylene and glass beads were tested. Gelatin was found to be the most effective adsorbent resulting in nearly 30% adsorption of cells initially present in suspension. Adsorption isotherm of cells on gelatin beads was represented by a three parameter expression due to sigmoidal shape of the isotherm. The constants of the adsorption isotherm were determined using the experimental data. 相似文献
10.
Summary Increased production of catharanthine (about 200 g/mL) was obtained at low light intensity under chemostat cultivation as compared with batch and fed-batch processes. Photo-inhibition was observed at high light intensity. Cells after being frozen with 5% DMSO produced more catharanthine (up to 350 g/L) in batch culture than those from conventional culture techniques. 相似文献
11.
12.
对野生植物长春花的营养成分进行了分析 ,结果表明 ,长春花中含有多种营养成分 ,其中至少含有 17种氨基酸。为开发利用植物资源提供了科学依据 相似文献
13.
14.
Hosokawa Keizo Matsuki Rikyu Oikawa Yayoi Yamamura Saburo 《Plant Cell, Tissue and Organ Culture》1997,51(2):137-140
Leaf sections of greenhouse-grown Miscanthus x ogiformis Honda 'Giganteus' plants and leaf sections or shoot apices of in
vitro shoot cultures were grown on Murashige and Skoog medium containing various concentrations of benzyladenine (BA) and
2,4-dichlorophenoxyacetic acid. On leaf sections, the callus induction decreased with increasing BA concentration. The percentage
of embryogenic callus was increased, the percentage of root-forming callus decreased, and a new shoot-forming callus type
was formed by inclusion of BA during callus induction. A higher percentage of shoot-forming callus was formed on shoot apices
compared with leaf sections of in vitro-grown shoots when cultured on 0.4 μM BA. The largest number of plants per callus piece
was regenerated from shoot-forming callus, but maintenance of the high regeneration capacity proved difficult.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
15.
Characterization of Alkaloid Uptake by Catharanthus roseus (L.) G. Don Protoplasts 总被引:1,自引:1,他引:0
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The accumulation of alkaloids by protoplasts of Catharanthus roseus (L.) G. Don var. Little Bright Eye was studied to determine the specificity of uptake and the role of ion trapping in the storage of alkaloids. Accumulation of the indole alkaloids vindoline, ajmalicine, tabersonine, and vinblastine was found to be biphasic, with an initial burst of uptake followed by a slow, prolonged phase of accumulation. The concentration and pH dependence of the initial burst of uptake for vindoline suggested that uptake occurred by simple diffusion. Uptake of nicotine was monophasic, with a half life of 5.2 minutes. The accumulation ratio (Ci/Ce) for nicotine at steady state and for the initial burst of uptake for vindoline and ajmalicine suggested that accumulation was driven by the pH gradient between the vacuole and the external assay medium. The second, sustained phase of uptake of vindoline was sensitive to inhibition by either 20 millimolar NaN3 or 0.5 millimolar Cu2+. In azide-treated protoplasts, the uptake for vindoline conformed to the kinetics of simple diffusion, with a half life of 4 minutes. The second phase of uptake for ajmalicine, although sensitive to inhibition by Cu2+, was insensitive to inhibition by NaN3. The biphasic uptake of the indole alkaloids was not due to any significant metabolism. It is concluded that accumulation and storage of the indole alkaloids is due only partly to ion trapping of the alkaloids by the low pH of the vacuole lumen. In the case of vindoline, there appears to be a specific energy-requiring uptake that is not seen with nicotine (which is not endogenous to Catharanthus). Accumulation of ajmalicine appears to involve both ion trapping and an azide-insensitive component, which may be due to complexation with organic counterions and phenolics. 相似文献
16.
We established an efficient plant regeneration system for Catharanthus roseus L. (G.) Don through somatic embryogenesis. Embryogenic callus was induced from hypocotyl of seed germinated in vitro. Somatic
embryogenesis in Catharanthus has been categorized into three distinct stages: (1) initiation and proliferation of embryo; (2) maturation, and; (3) germination
or plantlet conversion. Beside plant growth regulators, various stages of embryogenesis were screened for their response to
a wide variety of factors (pH, gelrite, light, sugar alcohols, polyethyleneglycol and amino acids), which affect embryogenesis.
All of the tested factors had a small to marked influence on embryogeny and eventual conversion to plantlets. The plantlets
were acclimatized successfully in a greenhouse. To our knowledge, this is the first report describing a detailed study of
various cultural factors which regulate embryogenesis in C. roseus. The results discussed in this paper may be used in mass propagation to produce medicinal raw material, and the embryo precursor
cells could be used in genetic modification programmes that aim to improve the alkaloid yield as well. 相似文献
17.
Mesophyll derived protoplasts ofCatharanthus roseus cv. Little pinkie were fused with protoplasts derived from an habituated cell line ofC. roseus. Polyethylene glycol was used as agglutinating agent while fusions were induced by square pulses. Best results were obtained
by fusing protoplasts from primary leaves with those from three-day-old cell cultures. Adding calcium ions considerably enhanced
heterofusion rate. Good cell viabilities indicated that this fusion process was not cytotoxic. The heterofusion frequency
was up to 10% or more. Most of the heterokaryons were able to regenerate their cell walls and underwent division.
Communicated by J. TUPY 相似文献
18.
19.
The effects of different combinations of plant growth regulators and light intensity on the formation of multiple shoots of Catharanthus roseus (L.) were studied. By composing three dimension surfaces and their topo views from experimental data, it was clear that Murashige-Shoog (MS) medium supplemented with 7.0 mg l-1 BA and 1.0 mg l-1 NAA strongly stimulated the formation of shoots, whereas medium supplemented with 2,4-d suppressed the formation of shoots or caused shoot dedifferentiated. Light intensities of 550–700 Lux were found to be beneficial to the formation of shoots when MS medium was supplemented with 2 mg l-1 6-BA and 0–1.0mg l-1 NAA.Abbreviations BA-6
benzyladenine
- NAA
-naphthalenacetic acid
- 2,4-d
2,4-dichlorophenoxyacetic acid 相似文献
20.
José Luis Cabrera-Ponce Ariadne Vegas-Garcia Luis Herrera-Estrella 《In vitro cellular & developmental biology. Plant》1996,32(2):86-90
AnAgrobacterium rhizogenes-mediated procedure for transformation of papaya (Carica papaya) was developed. Transgenic plants were obtained from somatic embryos that spontaneously formed at the base of transformed
roots, induced from leaf discs infected withA. rhizogenes. Transformation was monitored by autonomous growth of roots and somatic embryos, resistance to kanamycin, β-glucuronidase
activity (GUS), and Southern hybridization analysis. Over one-third of the infected leaf explants produced transformed roots
with GUS activity, from which 10% spontaneously produced somatic embryos. Histological analysis ofA. rhizogenes-transformed embryos showed that they have an altered symmetry between the shoot apex and the root meristem when compared
to somatic embryos induced with hormone treatment from control explants. Transgenic papaya plants containingA. rhizogenes rol genes were more sensitive to auxins, developed wrinkled leaves, and grew slower than nontransformed plants. 相似文献