Immobilization of Candida rugosa lipase on sporopollenin from Lycopodium clavatum

Sporopollenin is a natural polymer obtained from Lycopodium clavatum, which is highly stable with constant chemical structure and has high resistant capacity to chemical attack. In this study, immobilization of lipase from Candida rugosa (CRL) on sporopollenin by adsorption method is reported for the first time. Besides this, the enzyme adsorption capacity, activity and thermal stability of immobilized enzyme have also been investigated. It has been observed that under the optimum conditions (Spo-E_(0.3)), the specific activity of the immobilized lipase on the sporopollenin by adsorption was 16.3 U/mg protein, which is 0.46 times less than that of the free lipase (35.6 U/mg protein). The pH and temperature of immobilized enzyme were optimized, which were 6.0 and 40 °C respectively. Kinetic parameters V_max and K_m were also determined for the immobilized lipase. It was observed that there is an increase of the K_m value (7.54 mM) and a decrease of the V_max value (145.0 U/mg-protein) comparing with that of the free lipase.     

Variations in levels of clonality among Populus nigra L. stands of different ages

The evolution of genotypic diversity with population age remains poorly explored in clonal plant populations despite the potential for important shifts to occur through the course of time. Woody sprouting species are particularly under-represented in studies investigating intra-specific variations in levels of clonality from one locality to the next and through time. In this study we sought to determine the incidence and frequency of replicate genotypes in natural Populus nigra L. (Salicaceae) stands of different ages. Ten stands of this woody riparian sprouting species were selected in each of three distinct age groups (young, middle-aged and old) along a 30 km stretch of the River Garonne (south-west France). Leaf samples were collected from 15 neighbouring trees in each stand (450 samples in total) and replicate genotypes were identified using five SSR markers. Replicate genotypes were identified in two-thirds of all stands sampled (i.e. 50 of young stands, 100 of middle-aged stands and 50% of old stands). Young stands had significantly fewer replicated genotypes than middle-aged or old stands, while middle-aged stands had the greatest number of replicated genotypes. Replicate genotypes were most often found to occur as nearest neighbours and formed relatively small, discrete units (i.e. 2–4 trees growing in close proximity to one another). This suggests that asexual regeneration frequently occurs through flood-training in this species, although asexual regeneration from translocated fragments also evidently occurs as evidenced by 11 cases of replicate genotypes occurring in widely separated stands (up to 19 km apart). The results of this study highlight the need for a hierarchical sampling strategy in space and across age groups for an accureate understanding of the genotypic structure of woody sprouting species populations. Conservation and management of effective population sizes will benefit from better insight into not only spatial, but also temporal variations in levels of genotypic diversity.Co-ordinating editor: J. Tuomi     

小桐子糖原合成激酶基因JcGSK的克隆与表达分析

以小桐子(Jatropha curcas L.)cDNA为模版,克隆了JcGSK基因的CDS序列。序列分析表明,JcGSK基因包含1 230bp完全阅读框(ORF),编码409个氨基酸。预测其编码蛋白质的相对分子量为46.33kD,理论等电点为8.58。Blast搜索结果及进化分析结果表明,JcGSK蛋白与巴西橡胶树GSK蛋白的氨基酸序列一致性最高(94%)且亲缘关系最近;JcGSK基因编码的蛋白具有一个蛋白激酶特有的结构域。组织表达结果显示,JcGSK基因在小桐子根、茎、叶、花、果皮和种子中都有表达,且在根中表达量最高。小桐子幼苗在NaCl、ABA、PEG、低温和机械损伤处理后JcGSK基因表达量有不同程度的上调,推测其参与小桐子非生物胁迫响应和信号传导过程。JcGSK基因在种子中也有较高表达,在种子发育过程中表达量的变化与种子生长发育趋势基本一致,推测JcGSK基因也参与调控小桐子种子的生长发育。     

白菜型油菜RbohC和RbohF基因克隆与表达分析

该研究以白菜型油菜（Brassica rapa L.）‘陇油6号’为实验材料,采用RT PCR方法克隆油菜RbohC和RbohF基因,并采用实时荧光定量PCR技术对RbohC、RbohF基因在不同组织及非生物胁迫下的表达进行分析,为深入研究油菜RbohC、RbohF基因的生物学功能提供依据。结果显示：(1) 成功克隆得到2个全长分别为3 050 bp和2 995 bp的油菜RbohC (GenBank登录号：XM_009134386) 和RbohF (GenBank登录号：XM_009114548) 基因序列。(2) 生物信息学分析显示,油菜RbohC和RbohF基因开放阅读框（ORF）分别为2 733 bp和2 847 bp,编码910和948个氨基酸,推测二者的蛋白质分子量分别为103 kDa和108 kDa,理论等电点分别为9.47和9.21; 油菜RbohC、RbohF编码的氨基酸序列与萝卜等多种植物相应蛋白氨基酸序列具有较高的同源性,且这些序列高度保守并含有NADPH氧化酶的典型保守结构域,包括2个可以与Ca²⁺结合的EF手性模体结构、6个跨膜结构域、黄素腺嘌呤二核苷酸结合结构域、NAD焦磷酸结合结构域和C末端区域中的NADP核糖保守结合位点。(3) 油菜RbohC和RbohF基因在根、茎、叶和下胚轴中均表达,无组织特异性,但RbohC基因在根中表达量最高, RbohF基因在下胚轴中表达量最高。(4) 低温、干旱、盐、ABA、H₂O₂处理都能够诱导油菜RbohC和RbohF基因的表达,但抗寒性强的 ‘陇油6号’的RbohC和RbohF基因对胁迫的响应更敏感,且RbohC基因的表达量均高于RbohF基因。(5) 用H₂O₂清除剂DMTU、NADPH氧化酶抑制剂DPI和IMD、MAPKK抑制剂U0126处理后,油菜RbohC和RbohF基因的表达均较对照下降,说明U0126和DMTU对油菜RbohC和RbohF基因的表达有抑制作用。研究认为,油菜RbohC和RbohF基因在油菜适应逆境胁迫中具有重要作用,两基因的表达均受MAPK激酶信号途径的调节,并受到H₂O₂的反馈调节,而且抗寒性强的‘陇油6号’品种中RbohC和RbohF基因对H2O2和MAPK激酶信号途径的响应更敏感。     

苋菜AtGAI基因克隆及表达分析

为研究苋菜赤霉素不敏感基因(AtGAI)对赤霉素的响应,采用RT-PCR结合RACE技术,从‘大红’苋菜(Amaranthus tricolor L.‘Dahong’)中克隆得到一个GAI基因,命名为AtGAI(GenBank登录号为MK049175)。结果表明:(1)苋菜AtGAI基因含一个1 818bp开放阅读框,编码605个氨基酸。(2)生物信息学分析表明,苋菜AtGAI含2个保守结构域DELLA和GRAS,有GAI家族特有序列特征。(3)系统进化树分析表明,苋菜AtGAI蛋白与籽粒苋GAI蛋白亲缘关系最近。(4)显微观察及色素含量分析表明,甜菜色素主要分布于子叶和下胚轴的表皮及维管束鞘周围的薄壁细胞中,GA_3浓度与甜菜色素含量呈负相关。(5)qRT-PCR分析结果表明,GA_3抑制AtGAI、AmMYB1、AmaDODA和AmCYP76AD1基因在子叶和下胚轴中的表达。研究表明,GA_3可能通过影响AtGAI基因的表达来调控苋菜甜菜色素代谢。     

《广西植物志》秋海棠属(Begonia L.)增订

秋海棠属(Begonia L.)隶属于葫芦目(Cucurbitales)秋海棠科(Begoniaceae),有1 800余种,是世界第六大属,主要分布于美洲、非洲和亚洲的热带和亚热带地区,具有极高的观赏价值,亦可作为药用、食用、饮料和饲料等。我国有秋海棠属植物近300种,主要分布于我国的云南和广西等长江以南地区,其中云南有秋海棠属植物100余种。近年来,随着广西地区秋海棠属植物野外调查的不断深入,陆续发现并发表了大量秋海棠属新类群,使得广西产秋海棠属植物数目已超出1991年《广西植物志》(第一卷)收录的19种,因此亟需对发表于不同期刊中的类群进行系统地整理和汇总。鉴于此,该文通过查阅文献资料和考证标本信息,整理并汇总了广西产秋海棠属植物共84种,包括2个变种、11个亚种和46个特有种,新增的65种为《广西植物志》(第一卷)中未收录的类群,并补充了这些类群的特征集要、凭证标本信息和属下分组概况,为今后开展秋海棠属植物的分类修订以及系统学、进化生物学和保护生物学等研究提供了参考依据。     

Micromorphological and chemical characterisation of Stachys recta L. subsp. serpentini (Fiori) Arrigoni in comparison to Stachys recta L. subsp. recta (Lamiaceae)

Stachys recta L. is a very polymorphous species in which numerous subspecies were recognised. S. recta L. subsp. serpentini (Fiori) Arrigoni is a typical endemism growing on serpentine soils in northern Apennines and particularly in Tuscany (Italy). In order to contribute to a better knowledge of this plant and to its differentiation with respect to S. recta L. subsp. recta, the micromorphological characters (non-glandular and glandular trichomes) and the essential oil composition of the two subspecies were investigated. Micromorphological characters were studied using scanning and transmission electron microscopy, while light microscopy was used for histochemical observations. Essential oil analysis was carried out by gas chromatography and mass spectrometry.In the two examined taxa, the morphology and distribution of glandular and non-glandular trichomes, and the different essential oil composition, may be considered distinctive characters at subspecies level. This is consistent with the taxonomic classification considering S. recta subsp. serpentini a subordinate taxon of S. recta.     

Molecular analysis of theBg-rbg transposable element system ofZea mays L.

Summary The two components of theBg-rbg transposable element system of maize have been cloned. TheBg element, isolated from the mutable allelewx-m32 :: Bg is inserted in the intron of theWaxy (Wx) gene between exons 12 and 13. The length of the element is of 4869 bp.Bg has 5 by terminal inverted repeats, and generates upon insertion an 8 by direct duplication of the target sequence. Both ends of theBg element contain a 76 by direct repeat adjacent to the terminal inverted repeats. The hexamer motif TATCG_kC ^G is here repeated several times in direct or inverse orientation. Therbg element was isolated from the mutable alleleo2m(r) where it is located in the promoter region of theOpaque-2 (O2) gene.rbg is approximately 4.5 kb in length, has terminal inverted repeats identical to those of theBg element, and is also flanked by an 8 by direct duplication at the target site. LikeBg, rbg carries the 76 by direct repeats. Restriction enzyme analysis reveals that, compared toBg, the receptor element is distinguishable by small deletion and insertion events. Sequence data indicate that not more than 75% homology exists at the DNA level between therbg element and the autonomousBg element.     

小桐子JcCIPK2基因的克隆与表达分析

钙调磷酸酶B类似蛋白互作蛋白激酶(CBL-interacting protein kinase, CIPK)是一类植物中特有的丝氨酸/苏氨酸(Ser/Thr)蛋白激酶,参与多种生物和非生物胁迫响应过程。该实验通过RT-PCR克隆了小桐子(Jatropha curcas L.)JcCIPK2基因cDNA全长序列,采用荧光定量qRT-PCR分析JcCIPK2基因在不同组织及不同处理(12℃和1℃低温、42℃高温、30%PEG、250 mmol/L NaCl、150μmol/L ABA)下的表达模式。结果显示:(1)小桐子JcCIPK2基因开放阅读框全长1 398 bp,编码465个氨基酸,相对分子量为52.95 kD,等电点为8.89。(2)蛋白质结构分析表明,JcCIPK2的N端含有位于第11～265个氨基酸之间的丝氨酸/苏氨酸激酶_蔗糖非发酵-1型相关蛋白激酶3催化域STKc_SnRK3,在激酶结构域内还具有激活环(Activation Loop);C端含有位于第316～430个氨基酸之间的CIPK蛋白激酶调控域CIPK_C,其调控域中含有CIPK家族典型的能与CBL特异性结合的NAF结构域,位于第314～369个氨基酸之间。(3)系统进化分析显示,小桐子JcCIPK2蛋白与同属于大戟科的木薯(Manihot esculenta Crantz.)同源关系最近,序列一致性达87%。(4)qRT-PCR分析表明,JcCIPK2基因在小桐子根、茎、叶中均有表达,经12℃和1℃低温处理后,叶片中JcCIPK2基因的表达都呈现先上调后下调表达的趋势,且都在低温处理24 h的表达量最高,与对照相比分别上调了6.0倍和16.72倍;小桐子JcCIPK2基因在42℃高温、30%PEG、150μmol/L ABA、250 mmol/L NaCl处理下也受到不同程度的诱导表达。研究推测,JcCIPK2基因在小桐子对逆境的响应与适应中起重要作用。     

菜心组织培养技术初探

为建立菜心(Brassica campestris ssp.chinensis var.utilis)的快繁技术体系,以花药和子叶-子叶柄为外植体进行组织培养研究。结果表明,花药培养以选取未开放的花蕾为宜,且花柱略高于花瓣,此时小孢子多数处于单核靠边期。菜心花粉的萌发率不高,且秋冬季的花粉比夏季的萌发率高。菜心花药愈伤组织诱导培养基为:MS+1.0 mg L–1 KT+1.0 mg L–1 2,4-D+3%糖+6 g L–1琼脂+8%椰乳,不定芽诱导培养基为:MS+2.0 mg L–1 6-BA+0.5 mg L–1 NAA+1.0 g L–1活性炭+2%糖+6 g L–1琼脂或MS+2.0 mg L–1 ZT+0.5 mg L–1 IAA+0.5 g L–1 AgNO3+1.0 g L–1活性炭+2%糖+6 g L–1琼脂。花药培养的不定芽诱导率为36.7%,不定芽培养出现褐化现象,不能形成再生植株;而以子叶-子叶柄为外植体培养获得的植株再生率可达80%。     

淫羊藿属植物的数量分类学研究

通过观测淫羊藿属植物的30个质量性状和15个数量性状,利用聚类分析和主成分分析的方法研究淫羊藿属属下类群的分类关系。结果表明:(1)聚类分析结果将淫羊藿属中国种类划分为大花类群和小花类群,支持Stearn对Sect.Macroceras、Sect.Polyphyllon和Subg.Rhizophyllum的处理,但认为Sect.Epimedium的分类地位尚需进一步探讨。(2)主成分分析结果显示,性状的累积贡献率不是很高,前3个主成分累积贡献率为51.86%,这可能与本属植物演化过程中性状变异的多样化和复杂化相关,但由主成分分析的结果仍可以看出中国种类被划分为大花类群和小花类群。研究认为,花瓣与内萼片长度比、花瓣是否具距、萼片轮数等作为主成分反映的性状对淫羊藿属分类具有重要价值。     

呼伦贝尔黄花苜蓿MfMYB30基因的克隆及表达分析

该研究在呼伦贝尔黄花苜蓿(Medicago falcata L. cv. Hulunbuir)转录组测序基础上,通过RT PCR方法克隆获得了MfMYB30基因,并通过生物信息学和表达分析进行初步研究,为深入研究MfMYB30基因的功能和开发利用奠定基础。结果表明：(1)成功克隆获得呼伦贝尔黄花苜蓿MfMYB30基因,其ORF序列长为957 bp,编码318个氨基酸,相对分子质量为86.85 kD,理论等电点为5.11。MfMYB30蛋白为疏水性蛋白,无跨膜结构,无信号肽序列。(2)系统进化分析表明黄花苜蓿MfMYB30蛋白与紫花苜蓿MsMYB4、拟南芥AtMYB30、木豆CcMYB30、蒺藜苜蓿MtMYB30和大豆GmMYB60聚为一个类群,亲缘关系较近。(3)实时荧光定量PCR结果显示,MfMYB30在黄花苜蓿模拟刈割不同天数后的相对表达量呈先降低后升高的趋势,在刈割7 d后相对表达量达到峰值。(4)通过在拟南芥原生质体亚细胞定位分析发现,该蛋白定位于细胞核。研究推测,MfMYB30基因可能在黄花苜蓿刈割或放牧胁迫响应过程中发挥重要调控作用。     

Chemical compositions of Casuarina equisetifolia L., Eucalyptus toreliana L. and Ficus elastica Roxb. ex Hornem cultivated in Nigeria

Essential oils were obtained by separate hydrodistillation of three different plants cultivated in Nigeria and analysed comprehensively for their constituents by means of gas chromatography (GC) and gas chromatography-mass spectrometry (GC–MS). The leaf essential oil of Casuarina equisetifolia L. (Casuarinaceae) comprised mainly of pentadecanal (32.0%) and 1,8-cineole (13.1%), with significant amounts of apiole (7.2%), α-phellandrene (7.0%) and α-terpinene (6.9%), while the fruit oil was dominated by caryophyllene-oxide (11.7%), trans-linalool oxide (11.5%), 1,8-cineole (9.7%), α-terpineol (8.8%) and α-pinene (8.5%). On the other hand, 1,8-cineole (39.4%) and α-terpinyl acetate (10.7%) occurred in large quantities in the essential oils of the leaf of Eucalyptus toreliana L. (Myrtaceae). The oil also features high levels of sabinene (5.9%), caryophyllene-oxide (4.7%) and α-pinene (4.2%). The main compounds identified in the leaf oil of Ficus elastica Roxb. ex Hornem. (Moraceae) were 6,10,14-trimethyl-2-pentadecanone (25.9%), geranyl acetone (9.9%), heneicosene (8.4%) and 1,8-cineole (8.2%).     

StP5CS基因转化结球甘蓝的研究

为研究StP5CS基因在结球甘蓝中的耐盐作用,以结球甘蓝下胚轴为外植体,采用农杆菌介导法将耐盐基因StP5CS和抗除草剂Bar基因导入结球甘蓝基因组中,在双丙氨膦的筛选下扩繁、生根,共获得了36株抗性植株。PCR扩增和Southern印迹杂交检测表明:目的基因StP5CS和Bar基因已经成功导入结球甘蓝基因组中。RT-PCR检测表明:StP5CS基因在转录水平也有表达。转基因植株耐盐试验结果显示:高浓度盐处理(400mmol/L NaCl)下,对照植株整株枯死,而转基因植株仍能正常生长;转基因植株的SOD活性、脯氨酸含量和相对膜透性均随盐浓度的升高呈上升趋势,均在400mmol/L NaCl处理下达到最大。结果表明转基因植株对高盐环境有一定的耐受性。     

Presence of Rhizobium Etli bv . Phaseoli and Rhizobium Gallicum bv . Gallicum in Egyptian Soils

Seven bean rhizobial strains EBRI 2, 3, 21, 24, 26, 27 and 29 identified as Rhizobium etli, and EBRI 32 identified as Rhizobium gallicum, isolated from Egyptian soils and which nodulated Phaseolus vulgaris efficiently, were subjected to hybridization with a nifH probe in order to estimate the copy number of this gene. Seven strains (EBRI 2, 3, 21, 24, 26, 27 and 29) which were only able to nodulate Phaseolus vulgaris, contained three copies of the nifH gene, consistent with their identification as Rhizobium etli bv. phaseoli. Only one strain (EBRI 32) which nodulated both Phaseolus vulgaris and Leucaena leucocephala, had one copy of nifH gene. This confirmed the classification of this strain as Rhizobium gallicum bv. gallicum.     

青稞HvBAS1基因的克隆及表达分析

细胞色素P450单加氧酶(CYP450)是参与植物代谢的最大酶家族,其中CYP734A亚家族成员广泛参与植物激素油菜素类固醇(BRs)的失活。该研究以青稞农家品种‘肚里黄’幼苗为实验材料,通过人工合成激素24 表油菜素内酯(24 eBL)和BRs合成抑制剂油菜素唑(BRZ)处理,分析BRs对青藏高原特色作物青稞(Hordeum vulgare L. var. nudum Hook. f.)的影响;采用RT PCR技术从青稞中克隆HvBAS1基因,并运用实时定量PCR检测其表达特征,为深入分析HvBAS1基因的功能奠定研究基础。结果显示：(1)24 eBL能够显著促进青稞幼苗的生长,而BRZ处理后幼苗长势明显减缓。(2)从青稞中成功克隆到2个与拟南芥BRs失活基因BAS1高度同源的CYP734A亚家族基因,即HvBAS1 1和HvBAS1 2;HvBAS1 1开放阅读框全长1 629 bp,编码542个氨基酸;HvBAS1 2长1 689 bp,编码562个氨基酸,二者氨基酸序列相似性为76.42%;亚细胞定位预测结果显示二者均存在于内质网。(3)实时定量PCR检测发现,青稞HvBAS1 1与HvBAS1 2的表达模式完全不同,其中HvBAS1 1在青稞根中的表达量高于叶中,而HvBAS1 2在叶中的表达量高于根,且随着苗龄的增大,HvBAS1 1在根中的表达呈先升高后降低的趋势,HvBAS1 2在叶片中呈先降低后升高的变化趋势;BRZ处理青稞幼苗后,其HvBAS1 1与HvBAS1 2基因的表达较对照均显著下调,且HvBAS1 2下调更为明显。研究表明,HvBAS1 1和HvBAS1 2很可能都参与了青稞内源BRs的失活,但二者的功能存在差异。     

紫花苜蓿UV-B光受体基因MsUVR8的克隆及功能研究北大核心CSCD

该研究采用RACE扩增技术克隆了一个紫花苜蓿UV-B光受体基因(MsUVR8),在生物信息学分析基础上,采用农杆菌介导法获得了该基因过表达愈伤组织,并对UV-B辐射处理后MsUVR8过表达愈伤组织及其野生型中的类黄酮、黄酮醇、花青素、过氧化氢(H_(2)O_(2))、超氧阴离子(O_(2)^(-·))含量以及UV-B信号通路相关基因的表达进行检测分析,以探讨MsUVR8基因的生物学功能,为揭示植物响应UV-B胁迫的分子机制奠定理论基础。结果表明:(1)成功克隆获得紫花苜蓿MsUVR8基因CDS序列834 bp,且MsUVR8与蒺藜苜蓿MtUVR8基因序列相似度高达95%以上;MsUVR8蛋白形成了不完整的β-折叠结构,系统发育分析显示其与鹰嘴豆属于同一分支。(2)对MsUVR8过表达系检测发现,紫花苜蓿MsUVR8过表达愈伤组织(UVR8-OE)中类黄酮含量较野生型愈伤组织(WT)明显升高,而且经UV-B辐射后的UVR8-OE类黄酮物质含量较WT进一步显著升高。(3)DPBA荧光标记实验发现,UV-B辐射大大促进了细胞中黄酮醇的合成,且UV-B辐射后的UVR8-OE中黄酮醇含量最高。(4)DAB和NBT染色显示,UV-B处理后WT中活性氧(H_(2)O_(2)和O_(2)^(-·))的积累增加,而在UV-B辐射处理与未处理的UVR8-OE中H_(2)O_(2)和O_(2)^(-·)的积累无明显差异,表明MsUVR8可增强植物组织细胞的抗氧化性能,并可降低UV-B胁迫引起的氧化损伤。(5)UV-B辐照后,WT中PAL、CHS和FLS表达被激活而显著提高,UVR8-OE中的4种基因表达均达到最大,且较其他3个处理组均显著增强。研究认为,紫花苜蓿MsUVR8被UV-B激活后,促进了类黄酮合成相关基因的表达,并激活了类黄酮合成关键酶的活性,从而提高了类黄酮物质的合成效率,增强了UV-B胁迫条件下植物愈伤组织的抗氧化能力。     

油菜花粉发育相关基因RALFbn的克隆与表达分析

根据美国NCBI数据库中快速碱化因子(RALF)类基因序列的已知信息,克隆了油菜的快速碱化因子基因RALFbn,对其核酸序列及预测蛋白进行了生物信息学分析,并在油菜多种组织内观测其表达情况.结果表明:(1)经克隆获得油菜RALFbn基因的cDNA序列全长为510 bp,无内含子,编码79个氨基酸.(2)生物信息学分析发现,油菜RALFbn蛋白具有RALF类蛋白保守的“YIXY”区和4个保守的半胱氨酸残基,并且含有N豆蔻酰化位点、酪氨酸激酶Ⅱ磷酸化位点、蛋白激酶C磷酸化位点、和酪氨酸激酶磷酸化位点等多个生物活性位点,说明该蛋白在油菜中潜在的生理调节能力较为活跃.(3) RT-PCR检测RALFbn基因在油菜生殖器官中的表达结果发现,RALFbn主要在油菜雄蕊中表达,而在雌蕊、花瓣和萼片中没有表达.提示RALFbn基因极可能与油菜雄蕊中花粉的发育相关.     

线果芥CpNSP5基因的克隆及表达分析

以该实验室前期从线果芥(Conringia planisiliqua L.)中通过mRNA差异显示技术筛选到的414bp干旱响应相关核心片段为基础,采用RACE技术对该片段进行全长克隆,序列比对分析发现,该片段与拟南芥的AtNSP5基因相似性较高,命名为CpNSP5。CpNSP5基因全长1 228bp,开放阅读框966bp,编码321个氨基酸。推测蛋白分子量为35.034 5kD,等电点为5.41。蛋白二级结构预测包含26个β-折叠,具有典型的Kelch repeat结构。系统进化分析发现,CpNSP5与白菜亲缘关系最近。实时荧光定量PCR检测发现,CpNSP5基因在20%PEG-6000和200mmol·L~(-1) NaCl胁迫下均受到不同程度诱导,说明CpNSP5基因可能与线果芥响应逆境胁迫有关。该研究结果为作物的抗旱育种提供了候选基因。