Capacity for protein synthesis following heat stimulus of Drosophila associates with heat tolerance but does not underlie the latitudinal tolerance cline

Across populations of Drosophila melanogaster along the Australian eastern coastline latitudinal clines occur in both heat-knockdown tolerance and hardened heat-knockdown tolerance – low latitude tropical populations being more tolerant. A latitudinal cline also occurs for rates of total protein synthesis following a mild heat stress, with tropical populations having higher rates. Since the control of protein synthesis following heat stress is an important component of the cellular heat-shock response, we hypothesised that the higher rates of synthesis that follow a heat stimulus lead to higher knockdown tolerance and underpins the cline. However, levels of heat-stimulated total protein synthesis have been negatively related to heat-hardening capacity, a somewhat conflicting result. Here we examine the relationship between these physiological and adaptive traits in a set of 40 family lines derived from a hybrid laboratory population established by crossing populations from either end of the latitudinal transect. Among these lines high levels of heat-stimulated total protein synthesis were associated with both low basal and low heat-hardened adult knockdown time, confirming the importance of a negative relationship between protein synthesis and thermal tolerance. This result, when considered along with the directions of the latitudinal clines in protein synthesis and tolerance, suggests that variation in rates of heat-stimulated total protein synthesis is not a factor contributing to the latitudinal cline in heat tolerance. Given the robustness of this negative relationship we discuss possible explanations and future experiments to elucidate how the cellular heat stress response might facilitate increased knockdown tolerance.     

Geographical distribution and phenotypic differentiation in populations of Dactylis glomerata L. in Japan

Although the perennial grass Dactylis glomerata L. has established dominant populations in Japan since its introduction in the 1870s, there have been marked reductions in its abundance in southern and northeastern regions. In order to examine the effects of climatic factors on distribution and differentiation of the naturalized populations of D. glomerata, abundance of 26 populations over a distance of 1500 km along a latitudinal gradient was recorded at each site, and life-history traits of each population were measured in a common garden. It was found that the reduction in abundance was related to the mean summer temperature in southern regions and to the lowest temperature before snow cover in northeastern regions. Recent climatic records in Japan show an increase in the mean summer temperature but no apparent changes in the lowest temperature before snow cover. These data suggest that, assuming the recent trend in climatic changes continues, the population abundance will decrease in southern regions and will change little in the northeastern regions. Germination speed, leaf width and reproductive allocations showed clinal variation over a geographical range, and the southern populations had more rapid germination, narrower leaves and lower reproductive allocation than did the northern populations. On the other hand, seed size and germination date showed margin-center differentiation. Marginal populations in both distributional borders had smaller seeds and lower germination rates than did the central populations. This revised version was published online in June 2006 with corrections to the Cover Date.     

Cold activity and tolerance of the entomopathogenic fungus Tolypocladium spp. to UV-B irradiation and heat

Studies on the stress resistance of insect-pathogenic fungi are very important to better understand the survival of these organisms in the environment. In this study, we examined the cold activity (8 ± 1 °C for 7 days), UV-B tolerance (Quaite-weighted UV-B irradiance at 847.90 mW m⁻² for 1, 2, 3, and 4 h), and wet-heat tolerance (45 °C for 1, 2, 3, and 4 h) of two isolates of Tolypocladiumcylindrosporum (ARSEF 3392 and 5558), one isolate of Tolypocladium geodes (ARSEF 3275), and two isolates of Tolypocladium inflatum (ARSEF 4772 and 4877) based on their germination, compared with Metarhizium robertsii (ARSEF 2575). After 3 h of UV-B exposure, T. cylindrosporum germinated at a greater rate than the other Tolypocladium species and had similar viability to that of the M. robertsii. Most Tolypocladium isolates, however, were less UV-B tolerant than M. robertsii. The T.cylindrosporum isolates were also the most thermotolerant, with similar tolerance to the M. robertsii. The isolates of T. inflatum and T. geodes, which had similar heat tolerance, were the least heat tolerant compared with the isolates of T. cylindrosporum and M. robertsii. After 4 h of heat exposure, the germination of T. inflatum and T. geodes isolates was not significantly different. For cold activity, both T.cylindrosporum isolates germinated to ca. 100% in only 3 days. Approximately 50% of the two T. inflatum isolates germinated, and less than 5% of T. geodes germinated after 3 days. All fungal isolates, however, completely germinated by the seventh day, except M.robertsii. The isolates of T. cylindrosporum, therefore, were the most heat and UV-B tolerant, and had the highest cold activity compared to the other species. The tolerance of M. robertsii to UV-B radiation and heat was similar to that of T.cylindrosporum.     

Visual ecology of flies with particular reference to colour vision and colour preferences

The visual ecology of flies is outstanding among insects due to a combination of specific attributes. Flies’ compound eyes possess an open rhabdom and thus separate rhabdomeres in each ommatidium assigned to two visual pathways. The highly sensitive, monovariant neural superposition system is based on the excitation of the peripheral rhabdomeres of the retinula cells R1–6 and controls optomotor reactions. The two forms of central rhabdomeres of R7/8 retinula cells in each ommatidium build up a system with four photoreceptors sensitive in different wavelength ranges and thought to account for colour vision. Evidence from wavelength discrimination tests suggests that all colour stimuli are assigned to one of just four colour categories, but cooperation of the two pathways is also evident. Flies use colour cues for various behavioural reactions such as flower visitation, proboscis extension, host finding, and egg deposition. Direct evidence for colour vision, the ability to discriminate colours according to spectral shape but independent of intensity, has been demonstrated for few fly species only. Indirect evidence for colour vision provided from electrophysiological recordings of the spectral sensitivity of photoreceptors and opsin genes indicates similar requisites in various flies; the flies’ responses to coloured targets, however, are much more diverse.     

Thermal tolerance in a south-east African population of the tsetse fly Glossina pallidipes (Diptera, Glossinidae): implications for forecasting climate change impacts

For tsetse (Glossina spp.), the vectors of human and animal trypanosomiases, the physiological mechanisms linking variation in population dynamics with changing weather conditions have not been well established. Here, we investigate high- and low-temperature tolerance in terms of activity limits and survival in a natural population of adult Glossina pallidipes from eastern Zambia. Due to increased interest in chilling flies for handling and aerial dispersal in sterile insect technique control and eradication programmes, we also provide further detailed investigation of low-temperature responses. In wild-caught G. pallidipes, the probability of survival for 50% of the population at low-temperatures was at 3.7, 8.9 and 9.6 degrees C (95% CIs: +/-1.5 degrees C) for 1, 2 and 3 h treatments, respectively. At high temperatures, it was estimated that treatments at 37.9, 36.2 and 35.6 degrees C (95% CIs: +/-0.5 degrees C) would yield 50% population survival for 1, 2 and 3 h, respectively. Significant effects of time and temperature were detected at both temperature extremes (GLZ, p<0.05 in all cases) although a time-temperature interaction was only detected at high temperatures (p<0.0001). We synthesized data from four other Kenyan populations and found that upper critical thermal limits showed little variation among populations and laboratory treatments (range: 43.9-45.0 degrees C; 0.25 degrees C/min heating rate), although reduction to more ecologically relevant heating rates (0.06 degrees C/min) reduce these values significantly from approximately 44.4 to 40.6 degrees C, thereby providing a causal explanation for why tsetse distribution may be high-temperature limited. By contrast, low-temperature limits showed substantial variation among populations and acclimation treatments (range: 4.5-13.8 degrees C; 0.25 degrees C/min), indicating high levels of inter-population variability. Ecologically relevant cooling rates (0.06 degrees C/min) suggest tsetses are likely to experience chill coma temperatures under natural conditions (approximately 20-21 degrees C). The results from acute hardening experiments in the Zambian population demonstrate limited ability to improve low-temperature tolerance over short (hourly) timescales after non-lethal pre-treatments. In flies which survived chilling, recovery times were non-linear with plateaus between 2-6 and 8-12 degrees C. Survival times ranged between 4 and 36 h and did not vary between flies which had undergone chill coma by comparison with flies which had not, even after factoring body condition into the analyses (p>0.5 in all cases). However, flies with low chill coma values had the highest body water and fat content, indicating that when energy reserves are depleted, low-temperature tolerance may be compromised. Overall, these results suggest that physiological mechanisms may provide insight into tsetse population dynamics, hence distribution and abundance, and support a general prediction for reduced geographic distribution under future climate warming scenarios.     

Effects of clonal variation of the host plant,interspecific competition,and climate on the population size of a folivorous thrips

Summary The effects of clonal variation, interspecific competition, and climate upon the population size of Apterothrips secticornis was assessed by a series of observations and experimental manipulations. Three clones of the host plant, Erigeron glaucus, consistently supported different numbers of thrips during monthly censuses. When rosettes of the three clones were transplanted to a common garden, relative numbers of thrips on the clones remained the same as those observed where the clones grew in situ. The presence or absence of other hervivores had no effect on thrips numbers in the common garden. Plume moth caterpillars and thrips were observed to co-occur less often than expected in the field but this was caused by differences in habitat selection by these two species rather than being the result of interspecific competition. Populations of thrips were affected by climate, but analyses suggest that the host clone was a more important factor.     

Surface chemical studies of Thiobacillus ferrooxidans with reference to copper tolerance

A strain of Thiobacillus ferrooxidans was adapted to grow at higher concentrations of copper by single step culturing in the presence of 20 g/L (0.314 mol/L) cupric ions added to 9K medium. Exposure to copper results in change in the surface chemistry of the microorganism. The isoelectric point of the adapted strain (pI=4.7) was observed to be at a higher pH than that of the wild unadapted strain(pI=2.0). Compared to the wild strain, the copper adapted strain was found to be more hydrophobic and showed enhanced attachment efficiency to the pyrite mineral. The copper adsorption ability of the adapted strain was also found to be higher than that of the wild strain. Fourier transform infrared spectroscopy of adapted cells suggested that a proteinaceous new cell surface component is synthesized by the adapted strain. Treatment of adapted cells with proteinase-K, resulted in complete loss of tolerance to copper, reduction in copper adsorption and hydrophobicity of the adapted cells. These observations strongly suggest a role played by cell surface modifications of Thiobacillus ferrooxidans in imparting the copper tolerance to the cells and bioleaching of sulphide minerals.     

The response of two butterfly species to climatic variation at the edge of their range and the implications for poleward range shifts

To predict changes in species' distributions due to climate change we must understand populations at the poleward edge of species' ranges. Ecologists generally expect range shifts under climate change caused by the expansion of edge populations as peripheral conditions increasingly resemble the range core. We tested whether peripheral populations of two contrasting butterflies, a small-bodied specialist (Erynnis propertius) and a large-bodied generalist (Papilio zelicaon), respond favorably to warmer conditions. Performance of populations related to climate was evaluated in seven peripheral populations spanning 1.2 degrees latitude (160 km) using: (1) population density surveys, an indirect measure of site suitability; and (2) organismal fitness in translocation experiments. There was evidence that population density increased with temperature for P. zelicaon whose population density declined with latitude in 1 of 3 sample years. On the other hand, E. propertius showed a positive relationship of population density with latitude, apparently unrelated to climate or measured habitat variables. Translocation experiments showed increased larval production at increased temperatures for both species, and in P. zelicaon, larval production also increased under drier conditions. These findings suggest that both species may increase at their range edge with warming but the preference for core-like conditions may be stronger in P. zelicaon. Further, populations of E. propertius at the range boundary may be large enough to act as sources of colonists for range expansions, but range expansion in this species may be prevented by a lack of available host plants further north. In total, the species appear to respond differently to climate and other factors that vary latitudinally, factors that will likely affect poleward expansion.     

Growth and leaf physiology of monkeyflowers with different altitude ranges

Every species is limited both geographically and ecologically to a subset of available habitats, yet for many species the causes of distribution limits are unknown. Temperature is thought to be one of the primary determinants of species distributions along latitudinal and altitudinal gradients. This study examined leaf physiology and plant performance under contrasting temperature regimes of sister species of monkeyflower, Mimulus cardinalis and Mimulus lewisii (Phrymaceae), that differ in altitude distribution to test the hypothesis that temperature is the primary determinant of differences in fitness versus altitude. Each species attained greatest aboveground biomass, net photosynthetic rate, and effective quantum yield of photosystem II when grown under temperatures characteristic of the altitudinal range center. Although both species exhibited greater stem length, stomatal conductance, and intercellular CO₂ concentration in hot than in cold temperatures, these traits showed much greater reductions under cold temperature for M. cardinalis than for M. lewisii. Survival of M. lewisii was also sensitive to temperature, showing a striking decrease in hot temperatures. Within each temperature regime, the species native to that temperature displayed greatest growth and leaf physiological capacity. Populations from the altitude range center and range margin of each species were used to examine population differentiation, but central and marginal populations did not differ in most growth or leaf physiological responses to temperature. This study provides evidence that M. cardinalis and M. lewisii differ in survival, growth, and leaf physiology under temperature regimes characterizing their contrasting low and high altitude range centers, and suggests that the species’ altitude range limits may arise, in part, due to metabolic limitations on growth that ultimately decrease survival and limit reproduction.Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users.     

CLAP: A web-server for automatic classification of proteins with special reference to multi-domain proteins

Background

The function of a protein can be deciphered with higher accuracy from its structure than from its amino acid sequence. Due to the huge gap in the available protein sequence and structural space, tools that can generate functionally homogeneous clusters using only the sequence information, hold great importance. For this, traditional alignment-based tools work well in most cases and clustering is performed on the basis of sequence similarity. But, in the case of multi-domain proteins, the alignment quality might be poor due to varied lengths of the proteins, domain shuffling or circular permutations. Multi-domain proteins are ubiquitous in nature, hence alignment-free tools, which overcome the shortcomings of alignment-based protein comparison methods, are required. Further, existing tools classify proteins using only domain-level information and hence miss out on the information encoded in the tethered regions or accessory domains. Our method, on the other hand, takes into account the full-length sequence of a protein, consolidating the complete sequence information to understand a given protein better.

Results

Our web-server, CLAP (Classification of Proteins), is one such alignment-free software for automatic classification of protein sequences. It utilizes a pattern-matching algorithm that assigns local matching scores (LMS) to residues that are a part of the matched patterns between two sequences being compared. CLAP works on full-length sequences and does not require prior domain definitions.Pilot studies undertaken previously on protein kinases and immunoglobulins have shown that CLAP yields clusters, which have high functional and domain architectural similarity. Moreover, parsing at a statistically determined cut-off resulted in clusters that corroborated with the sub-family level classification of that particular domain family.

Conclusions

CLAP is a useful protein-clustering tool, independent of domain assignment, domain order, sequence length and domain diversity. Our method can be used for any set of protein sequences, yielding functionally relevant clusters with high domain architectural homogeneity. The CLAP web server is freely available for academic use at http://nslab.mbu.iisc.ernet.in/clap/.     

Flavonoids from Pinus sylvestris needles and their variation in trees of different origin grown for nearly a century at the same area

Flavonoids in needles of Scots pine planted in 1912–1914 in Poland from seeds originating from different parts of Europe, were isolated, chemically characterised and analysed by HPLC. It was shown that flavonoid profiles were similar in all tested populations and were different from those previously reported for Scots pine seedlings. They included taxifolin, taxifolin 3′-O-glucoside, quercetin as well as quercetin 3-O-glucoside and 3′-O-glucoside. The quercetin 3-O-glucoside could be found only in a trace amount in all samples and quercetin 3′-O-glucoside appeared in all samples regardless their origin. The relative concentration of taxifolin 3′-O-glucoside, quercetin, taxifolin and total flavonoids showed dependence on the origin of seeds; needles from high latitude populations contained smaller amounts of these compounds. Presented data clearly indicate that Scots pine contain glycosidases specific for glycosylation at C-3′ rather than at C-3. Besides, they indicate that long lasting influence of similar environmental factors is not able to change genetic regulatory systems responsible for flavonoid biosynthesis.     

5种杜鹃幼苗对高温胁迫的生理生化响应及耐热性综合评价

以杜鹃花属(Rhododendron L.)5个亚属5种杜鹃的4年生实生苗为材料,对模拟高温(30℃和38℃)条件下叶片丙二醛(MDA)、过氧化氢(H2O2)和脯氨酸(Pro)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性进行了测定分析,对各指标进行了相关性和主成分分析;在此基础上,采用隶属函数法对5种杜鹃的耐热性进行了综合评价,并通过建立最优回归方程对综合评价结果进行检验.测定结果表明:与对照( 22℃)相比,5种杜鹃幼苗的MDA、H2O2和Pro含量以及CAT和APX活性均随胁迫温度的升高而增加;SOD活性则表现为在30℃条件下小幅增加、38℃条件下下降的趋势.CAT活性与H2O2和Pro含量及APX活性均呈极显著正相关关系(P＜0.01),APX活性与Pro含量和SOD活性分别呈显著的正相关和负相关关系(P＜0.05).通过主成分分析可将测定的6个单项指标转换成3个相互独立的综合指标,累计贡献率达87.52％.根据综合评价值(D),可将5种杜鹃的耐热性分为4个等级:白花杜鹃[R.mucronatum (Blume)G.Don]的耐热性最强,毛棉杜鹃(R.moulmainense Hook.f.)和羊踯躅[R.molle (Blume)G.Don]耐热性较强,红滩杜鹃(R.chihsinanum Chun et Fang)耐热性较弱,红棕杜鹃(R.rubiginosum Franch.)的耐热性最弱.利用所建立的最优同归方程对供试种类进行耐热性预测,预测值与D值的次序完全一致,表明综合评价法可用于杜鹃苗期耐热性的评价.     

Concepts of aggregation and their quantification: A critical review with some new results and applications

Various indices of spatial patterns based on plot counts are reviewed for theoretical population models appropriate to ecological studies. It is seen that many of the indices proposed in the literature are essentially equivalent to either the index ω= σ²/μ or to the index γ= (σ²-μ)/μ², thus providing a variety of motiviations and interpretations of these two indices as measures of spatial patterns. A vector approach to measuring spatial patterns which suggests both a unifying relationship between these indices and an extension of them is proposed. This leads to an interpretation of the measures of spatial patterns in terms of the transition probabilities of a pure birth process.     

Thermometry and dosimetry of heat with specific reference to the liquid-crystal optical fiber temperature probe

Summary A temperature sensing probe was designed utilizing the unique optical properties of thermotropic liquid crystals. An electronic and fiberoptic system coupled to the sensor was used to monitor temperature-dependent interactions of light (660 nm) with the liquid crystal mixture. The probe is a nonmetallic, nonperturbing thermal sensor for continuous temperature measurements of biological specimens subjected to electromagnetic fields. The advantages and disadvantages of the Liquid Crystal Optical Fiber (LCOF) temperature probe are discussed along with some typical applications where accurate dosimetry and thermometry are important parameters in experimental studies.     

Genetic variability and large scale differentiation in two species of littorinid gastropods with planktotrophic development, Littorina littorea (L.) and Melarhaphe (Littorina) neritoides (L.) (Prosobranchia: Littorinacea), with notes on a mass occurrence of M. neritoides in Sweden

A planktotrophic larval development suggests a relatively high gene flow over long distances. Both Littorina littorea and Melarhaphe neritoides have an egg capsule and planktotrophic larvae which taken together are pelagic over a period of 4–8 weeks. In the absence of differential selection one would expect low levels of genetic differentiation over large distances in these two species. In this study of allozyme variation, low levels of differentiation were found over thousands of kilometres (Norway to Spain for L. littorea and Sweden to Greece for M. neritoides). This supports the hypothesis of two species with a high dispersal potential. A second expectation from neutral theory is that effective population size is positively correlated with average levels of genetic variation within species. In light of the generally high densities of local populations of these species and the high interpopulation migration rate, both L. littorea and M. neritoides may be considered as having high N_e s. Contrary to neutral expectation, L. littorea revealed very low levels of heterozygosity over its whole European range (mean H_exp= 0.015, 15 loci), while average heterozygosity of M. neritoides (H_exp= 0.084, 11 loci) was no more than in other littorinid species. This paper also reports the occurrence of M. neritoides on the Swedish west coast in 1988, 89 and 90, and two factors which may have promoted this unusually large invasion are discussed.     

Comparison of growth habits under various light conditions between two climax species, Castanopsis sieboldii and Castanopsis cuspidata, with special reference to their shade tolerance

Two experiments were conducted to examine the difference in shade tolerance between two climax species, Castanopsis sieboldii and Castanopsis cuspidata, in Aichi Prefecture in the warm-temperate climate of Japan. In the growth experiment, the seedlings of the two species were raised under five light conditions, 100%, 30%, 10%, 3% and 1% relative light intensity (RLI). The growth of C. sieboldii highly exceeded that of C. cuspidata, particularly under higher light conditions. In the heavy shading experiment, the seedlings of the two species were grown under three light conditions (0, 0.15 and 0.3% RLI). While all seedlings of C. sieboldii died within 2 years under 0.15 and 0.3% RLI, about half of the seedlings of C. cuspidata survived more than 2 years under 0.15% RLI, and about 70% survived more than 4 years under 0.3% RLI. It was concluded that C. cuspidata was more shade tolerant than C. sieboldii, and the possibility that C. sieboldii might be more tolerant of the drought stress than C. cuspidata was discussed.     

Nitrate uptake,nitrate reductase distribution and their relation to proton release in five nodulated grain legumes

Nitrate uptake, nitrate reductase activity (NRA) and net proton release were compared in five grain legumes grown at 0.2 and 2 mM nitrate in nutrient solution. Nitrate treatments, imposed on 22-d-old, fully nodulated plants, lasted for 21 d. Increasing nitrate supply did not significantly influence the growth of any of the species during the treatment, but yellow lupin (Lupinus luteus) had a higher growth rate than the other species examined. At 0.2 mM nitrate supply, nitrate uptake rates ranged from 0.6 to 1.5 mg N g(-1) d(-1) in the order: yellow lupin > field pea (Pisum sativum) > chickpea (Cicer arietinum) > narrow-leafed lupin (L angustifolius) > white lupin (L albus). At 2 mM nitrate supply, nitrate uptake ranged from 1.7 to 8.2 mg N g(-1) d(-1) in the order: field pea > chickpea > white lupin > yellow lupin > narrow-leafed lupin. Nitrate reductase activity increased with increased nitrate supply, with the majority of NRA being present in shoots. Field pea and chickpea had much higher shoot NRA than the three lupin species. When 0.2 mM nitrate was supplied, narrow-leafed lupinreleased the most H+ per unit root biomass per day, followed by yellow lupin, white lupin, field pea and chickpea. At 2 mM nitrate, narrow-leafed lupin and yellow lupin showed net proton release, whereas the other species, especially field pea, showed net OH- release. Irrespective of legume species and nitrate supply, proton release was negatively correlated with nitrate uptake and NRA in shoots, but not with NRA in roots.